81 results on '"De Lima S"'
Search Results
2. Cytokines and inflammatory mediators: Markers involved in interstitial damage to the pancreas in two dengue fever cases associated with acute pancreatitis
- Author
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Felipe de Andrade Vieira Alves, Lucca de Lima S. Oliveira, Natália Gedeão Salomão, David William Provance, Carlos Alberto Basilio-de-Oliveira, Rodrigo Basílio-de-Oliveira, Leandro Junqueira Moragas, Jorge José de Carvalho, Ronaldo Mohana-Borges, Kíssila Rabelo, and Marciano Viana Paes
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Male ,RNA viruses ,Viral Diseases ,Physiology ,viruses ,Apoptosis ,Pathology and Laboratory Medicine ,Biochemistry ,Dengue Fever ,Dengue ,White Blood Cells ,Medical Conditions ,Animal Cells ,Immune Physiology ,Medicine and Health Sciences ,Innate Immune System ,Multidisciplinary ,Middle Aged ,Infectious Diseases ,Medical Microbiology ,Viral Pathogens ,Viruses ,Cytokines ,Medicine ,Female ,Inflammation Mediators ,Pathogens ,Cellular Types ,Anatomy ,Research Article ,Neglected Tropical Diseases ,Adult ,Immune Cells ,Science ,Immunology ,Endocrine System ,Gastroenterology and Hepatology ,Microbiology ,Young Adult ,Exocrine Glands ,Humans ,Microbial Pathogens ,Pancreas ,Blood Cells ,Flaviviruses ,Macrophages ,Organisms ,Biology and Life Sciences ,Proteins ,Cell Biology ,Dengue Virus ,Molecular Development ,Tropical Diseases ,Fibrosis ,Pancreatitis ,Immune System ,Collagens ,Biomarkers ,Developmental Biology - Abstract
Dengue viral (DENV) infections can lead to acute pancreatitis and associated tissue damage. This study examined the pancreas from two fatal cases of DENV for histopathological changes as well as for the detection of cytokines, and other inflammatory mediators. Tissue sections were prepared for examination by ultrastructural and histopathological techniques. Sections from the pancreas of non-infected individuals were prepared in parallel as a control. The presence of viral replication in macrophages was detected by co-staining for the proteins NS3 and CD68 by immunofluorescence. Immunohistochemistry was used to detect cells that expressed cytokines and inflammatory mediators to characterize the inflammatory response. Edema, acinar necrosis and fibrosis areas associated with a mononuclear infiltrate were found in infected tissues. The major site of virus replication appeared to be macrophages based on their exclusive presentation of the viral protein NS3. Pancreatic tissues from the infected individuals also displayed increased levels of high mobility group box-1, caspase-3, gelatinase B and tumor necrosis factor alpha compared to controls. The presence of virus replicating macrophages in the pancreas was associated with multiple changes in tissue structure that included elevated levels of cytokines and inflammatory markers that may differentiate acute pancreatitis due to DENV infections from other causes.
- Published
- 2022
3. Nicotinamide as a biostimulant improves soybean growth and yield
- Author
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de Lima Sebastião Ferreira, Vendruscolo Eduardo Pradi, Alves Vitória Carolina Dantas, Arguelho Jeysielli Cristaldo, Pião Joice de Abreu, Seron Cássio de Castro, Martins Murilo Battistuzzi, Witt Travis Wilson, Serafim Gabriela Moura, and Contardi Lucymara Merquides
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vitamins ,niacin ,bioinputs ,glycine max ,Agriculture ,Agriculture (General) ,S1-972 - Abstract
Modern agriculture seeks, through technology, to increase agricultural productivity, aiming at greater sustainability during food production. The application of vitamins meets this objective, acting as a biostimulant for different cultivation conditions. In this sense, the aim of this research is to evaluate the effect of the foliar application of nicotinamide on the soybean crop in two growing environments. The treatments were composed of the nicotinamide concentrations of 0, 100, 200, 300, 400, 500, and 600 mg L−1 of water applied when the plants were at the R3 stage (beginning of pod formation), exogenously, by foliar spraying. The application of nicotinamide resulted in increases in the vegetative traits of plant height and the number of branches per plant by 9.7 and 7.8%, respectively. Regarding the productive characteristics, considering the average of the two cultivation sites, the application of the vitamin resulted in increments of about 15.62, 18.57, 20.53, 4.32, and 19.19% on the number of pods, number of grains, mass of grains per plant, mass of 1,000 grains, and yield, respectively. In this way, we conclude that nicotinamide, applied at concentrations ranging from 237.8 to 373.8 mg L−1, promotes benefits to soybean growth and yield in different environments, and can be considered as a biostimulant.
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- 2024
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4. Relationships of mineralized dermal layer of mountain endemic miniature frogs with climate.
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Mari, R. de B., Mori, G. M., Vannucchi, F. S., Ribeiro, L. F., Correa, C. N., de Lima, S. K. S., Teixeira, L., Sandretti‐Silva, G., Nadaline, J., and Bornschein, M. R.
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DEHYDRATION ,FROGS ,MOUNTAINS - Abstract
The mineralized dermal layer (MDL) is found in most terrestrial anurans. Its thickness represents on average up to 8% of that of the entire skin. It has been proposed that it may reduce body water loss, act on homeostasis, support skin structure, or conversely, it may be a currently functionless trait constrained by groups' evolutionary history. We described the MDL morphology of 11 Brachycephalus species, terrestrial, miniaturized and microendemic anurans, and tested for its relationship with climate of higher latitude regions of the Atlantic Rainforest of Brazil. All species presented MDL, described with two distinct morphological patterns: homogeneous or heterogeneous MDL, the latter distinguishable by MDL with dorsal or lateral expansion, sometimes up to the limit of the epidermis and comprising up to 50% of the thicknesses of the entire skin. Climate differed between locations by MDL morphological group, less rainy or seasonally less rainy where species with heterogeneous MDL occur. Our results indicate that the abundance of calcium in MDL and its heterogeneous condition suggest its adaptive function in reduce water loss. Such adaptations in anurans in very humid highlands reinforce the mountains' propensity for rapid loss of humidity, demystifying them as an extremely abundant source of water. This is the third study that tested the relationship between the MDL morphology and the environment where species occur and the first that correlated this structure with the climate of anurans of the same habit and distributed in a single habitat, the Atlantic Rainforest. [ABSTRACT FROM AUTHOR]
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- 2022
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5. Revising the M235T Polymorphism Position for the AGT Gene and Reporting a Modifying Variant in the Brazilian Population with Potential Cardiac and Neural Impact
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Lemos, R. R., de Lima, S. G., da Cunha, J. E. Gomes, Oliveira, D. F., de Souza, M. B. Rodrigues, Ayres, C. F. J., Albuquerque, M. F. P. M., and Oliveira, J. R. M.
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- 2012
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6. Kinetics of hepatitis A virus replication in vivo and in vitro using negative-strand quantitative PCR
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de Paula, V. S., Perse, A. S., Amado, L. A., de Morais, L. M., de Lima, S. M. B., Tourinho, R. S., Gaspar, A. M. C., and Pinto, M. A.
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- 2009
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7. La1−x Ca x NiO3 Perovskite Oxides: Characterization and Catalytic Reactivity in Dry Reforming of Methane
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de Lima, S. M., Peña, M. A., Fierro, J. L. G., and Assaf, J. M.
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- 2008
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8. Bismuth silicon oxide (Bi12SiO20-BSO) and bismuth titanium oxide (Bi12TiO20-BTO) obtained by mechanicalalloying
- Author
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Vasconcelos, I. F., De Figueiredo, R. S., Guedes De Lima, S. J., and Sombra, A. S. B.
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- 1999
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9. Composition and larvicidal activity of the essential oils of Lantana camara and Lantana montevidensis
- Author
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Costa, J. G. M., Rodrigues, F. F. G., Sousa, E. O., Junior, D. M. S., Campos, A. R., Coutinho, H. D. M., and de Lima, S. G.
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- 2010
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10. Cytokines and inflammatory mediators: Markers involved in interstitial damage to the pancreas in two dengue fever cases associated with acute pancreatitis.
- Author
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Alves, Felipe de Andrade Vieira, Oliveira, Lucca de Lima S., Salomão, Natália Gedeão, Provance Jr, David William, Basilio-de-Oliveira, Carlos Alberto, Basílio-de-Oliveira, Rodrigo, Moragas, Leandro Junqueira, de Carvalho, Jorge José, Mohana-Borges, Ronaldo, Rabelo, Kíssila, and Paes, Marciano Viana
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DENGUE , *INFLAMMATORY mediators , *TUMOR necrosis factors , *PANCREATITIS , *PANCREAS , *CYTOKINES , *VIRAL proteins - Abstract
Dengue viral (DENV) infections can lead to acute pancreatitis and associated tissue damage. This study examined the pancreas from two fatal cases of DENV for histopathological changes as well as for the detection of cytokines, and other inflammatory mediators. Tissue sections were prepared for examination by ultrastructural and histopathological techniques. Sections from the pancreas of non-infected individuals were prepared in parallel as a control. The presence of viral replication in macrophages was detected by co-staining for the proteins NS3 and CD68 by immunofluorescence. Immunohistochemistry was used to detect cells that expressed cytokines and inflammatory mediators to characterize the inflammatory response. Edema, acinar necrosis and fibrosis areas associated with a mononuclear infiltrate were found in infected tissues. The major site of virus replication appeared to be macrophages based on their exclusive presentation of the viral protein NS3. Pancreatic tissues from the infected individuals also displayed increased levels of high mobility group box-1, caspase-3, gelatinase B and tumor necrosis factor alpha compared to controls. The presence of virus replicating macrophages in the pancreas was associated with multiple changes in tissue structure that included elevated levels of cytokines and inflammatory markers that may differentiate acute pancreatitis due to DENV infections from other causes. [ABSTRACT FROM AUTHOR]
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- 2022
- Full Text
- View/download PDF
11. Rescuing axons from degeneration does not affect retinal ganglion cell death
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de Lima, S., Mietto, B.S., Paula, C., Muniz, T., Martinez, A.M.B., and Gardino, P.F.
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- 2016
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12. Introdução pelo Próprio de Corpos Estranhos na Uretra e Bexiga - Relato de Três Casos Pediátricos
- Author
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Ferreira de Lima, S, Cebola, A, Pereira, SC, and Alves, R
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Urethra ,HDE CIR PED ,Urinary Bladder ,Foreign Bodies ,Child - Abstract
Self-inserted urethrovesical foreign bodies are rare in children.We present three cases and discuss the clinical presentation, diagnosis and management of such patients. In case 1, a 16-year-old boy introduced a wire into the urethra and partially into the bladder three days before. In case 2, a 4-year-old boy introduced a hairpin in the urethra in the same day. In case 3, a 11-year-old boy introduced a sewing needle in the urethra a few hours before. Cystourethroscopy and suprapubic cystotomy were used to remove the foreign bodies. The presentation of urethrovesical foreign bodies can vary widely, as can the type of object inserted. Foreign body retrieval is determined by its morphology and the patient’s conditions with the aim to minimise urothelial trauma and preserve erectile function. Definitive treatment is usually the endoscopic removal, however sometimes surgical intervention may be required. It is advocated follow-up with long duration, which is necessary to diagnose the long-term complications including urethral stricture. info:eu-repo/semantics/publishedVersion
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- 2019
13. Seasonality and Dynamics of Gastrointestinal Helminths in Grazing Horses
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de Lima Saes Isabela, Gonçalves Juliana Alencar, do Carmo Tábata Alves, Mena Mateus Oliveira, de Almeida Cipriano Isabela, de Favare Giordani Mascoli, Bello Hornblenda Joaquina Silva, Guelpa Gabriel Jabismar, and de Soutello Ricardo Velludo Gomes
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equines ,nematodes ,prevalence ,susceptibility ,Veterinary medicine ,SF600-1100 - Abstract
The prevalence of helminths in horses may vary, depending on climatic factors and host susceptibility. The objective of this study was to evaluate the seasonal dynamics of helminth infection in grazing horses, from October 2015 to September 2016. In total, 104 crossbred horses of different age groups, males and females in a single herd, participated in this study. Individual fecal samples were collected every 28 days for counting of eggs per gram of feces (EPG) and fecal cultures. The horses were evaluated individually for weight, body condition score (BCS) and clinical status. Climatic information relating to the farm was also collected. The categories most affected by helminths were foals and senior horses, with EPG averages of 1271.9 and 1186.5, respectively. The EPG averages for all animal categories together, in the four seasons were: 1042.1 spring; 1508.9 summer; 817.8 autumn; and 571.1 winter. Through the body condition evaluation, it was seen that senior animals presented the lowest BCS in all seasons. It was concluded that the season in which these animals were most affected by helminth infections was the summer and that the most susceptible categories were foals and senior horses.
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- 2022
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14. Physicochemical and spectroscopical investigation of Pequi (Caryocar coriaceum Wittm.) pulp oil
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Sena, Diniz M., Rodrigues, Fabíola F.G., Freire, Paulo T.C., de Lima, S. G., Coutinho, Henrique D.M., Carvajal, Juan C.L., and da Costa, José G.M.
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Vibrational analysis ,Análisis vibracional ,Pequi ,Caracterización fisicoquímica ,caryocar coriaceum ,Composición del aceite ,Nutrition. Foods and food supply ,Caryocar coriaceum ,lcsh:TX341-641 ,Oil composition ,vibrational analysis ,pequi ,physicochemical characterization ,oil composition ,TX341-641 ,Physicochemical characterization ,lcsh:Nutrition. Foods and food supply - Abstract
This work describes the physicochemical characterization of the fruit pulp oil of Caryocar coriaceum (Wittm), “pequi.” The chemical composition was assessed by GLC, which demonstrated the following fatty acid contents: oleic (55.79%), palmitic (34.18%), heptadecenoic (5.86%), linoleic (1.80%), stearic (1.73%), eicosenoic (0.37%) and palmitoleic (0.27%). The vibrational spectroscopy results were typical of an edible oil, and are in accordance with the high unsaturated fatty acid content. Physical properties such as water content, acidity, peroxide index, saponification index, relative density, viscosity and refraction index are reported for the first time for this species. Pequi fruits have a high nutritional value, and are rich in protein and vitamins. In addition, their composition includes essential fatty acids, which accounts for the popular use of the fruit oil for antioxidant and antiinflammatory activity. There is great interest in extending the use of this natural product by exploiting the combined social, economic and therapeutic values of its derivatives.El presente trabajo describe la caracterización físico-química del aceite de la pulpa de Caryocar coriaceum (Wittm), “pequi”. La composición química fue evaluada por GLC, que mostró el siguiente contenido de ácido graso: oleico (55,79%), palmítico (34,18%), heptadecenoico (5,86%), linoleico (1,80%), esteárico (1,73%), eicosenoico (0,37%) y palmitoleico (0,27%). Los resultados de la espectroscopia vibracional fueron típicos de un aceite comestible, y están en concordancia con el alto contenido de ácido graso insaturado. Las propiedades físicas tales como el contenido de agua, la acidez, índice de peróxido, índice de saponificación, densidad relativa, viscosidad e índice de refracción se presentan por primera vez para esta especie. Los frutos de pequi tienen un alto valor nutricional, y son ricos en proteínas y vitaminas; además su composición incluye ácidos grasos esenciales. El aceite de la fruta tiene un uso popular debido a su actividad antioxidante y antiinflamatoria. Hay un gran interés en extender el uso de este producto natural aprovechando los valores sociales, económicos y terapéuticos de sus derivados.
- Published
- 2010
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15. Pathoanatomical findings of a green turtle (Chelonia mydas) victim of collision with a motorboat
- Author
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De Oliveira Radan Elvis Matias, Attademo Fernanda Loffler Niemeyer, Freire Augusto Carlos Da Bôaviagem, De Lorena Pires Juliana Maia, De Farias Daniel Solon Dias, Bomfim Aline Da Costa, De Oliveira Rysónely Maclay, Gavilan Simone Almeida, De Lima Silva Flávio José, and De Oliveira Moacir Franco
- Subjects
cheloniidae ,propeller ,testudines ,trauma ,Veterinary medicine ,SF600-1100 - Abstract
In this paper we report the trauma suffered by a green turtle (Chelonia mydas), caused by a collision with a motorboat, and describe the case ante and post-mortem. An adult female green turtle was rescued alive on December 2, 2016 at Ponta Negra beach, municipality of Natal, Rio Grande do Norte, Brazil. The rescued animal was sent to the Marine Fauna Rehabilitation Center (PCCB-UERN), municipality of Areia Branca (RN), in Brazil. Physical examination revealed an excellent body score, noisy breathing, moderate dehydration, absence of hind limb and cloacal reflexes when stimulated by pressure, and two traumatic injuries characteristic of a collision with a motorboat. After three days of supportive treatment, the animal died and was immediately sent for necropsy. The animal had a complete fracture of the vertebral bodies (dorsal elements D9 and D10) and spinal cord section. The entire gastrointestinal tract had food content, in addition to sharp injuries in the colon caused by the bone fragments of the fractured carapace. The lungs were congested, hemorrhagic with frothy and bloody secretion, and interstitial bronchiole fibrosis. There was also present some fibrin and a large number of leukocyte cells, consisting mainly of macrophages. The liver was enlarged, with rounded edges and thickening of the capsule, multifocal areas of hepatocellular necrosis, and dissociation of the hepatocyte cords. The collision resulted in the exposure of the coelomic cavity and spinal cord, causing the animal intense pain, paralysis of the hind limbs and cloaca, septicemia, and consequently, death.
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- 2021
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16. Kinetics of hepatitis A virus replication in vivo and in vitro using negative-strand quantitative PCR.
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Paula, V. S., Perse, A. S., Amado, L. A., de Morais, L. M., de Lima, S. M. B., Tourinho, R. S., Gaspar, A. M. C., and Pinto, M. A.
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HEPATITIS A virus ,RNA ,POLYMERASE chain reaction ,CELL culture ,DNA polymerases - Abstract
The replication of hepatitis A virus (HAV) is via a complementary negative-strand RNA. Each negative strand may serve as a template for the synthesis of many positive strands. The aim of this study was to detect the intermediate replicative (negative strand) of HAV in order to monitor its replication in vitro and in vivo. Real-time polymerase chain reaction (PCR) was standardized to detect the intermediate replicative of HAV in cell culture and liver from non-human primates infected experimentally. HAV primers from the 5′ non-translated region and VP3 were used in the cDNA synthesis of negative-strand RNA. The negative strand was detected in the infected cell lines and liver by highly strand-specific rT th recombinant Thermus thermophilus DNA polymerase reverse transcription followed by quantitative PCR. The results indicate that the negative-strand HAV RNA can be detected in vivo and in vitro. This model is an approach for assessing the dynamic patterns of replication and should represent a valuable tool for the monitoring of HAV replications in cell cultures and for the evaluation of experimental infections in animal models. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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17. La1− x Ca x NiO3 Perovskite Oxides: Characterization and Catalytic Reactivity in Dry Reforming of Methane.
- Author
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de Lima, S. M., Peña, M. A., Fierro, J. L. G., and Assaf, J. M.
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PEROVSKITE , *OXIDE minerals , *METHANE , *OXIDATION-reduction reaction , *NATURAL gas - Abstract
Perovskite-type oxides such as La1− x Ca x NiO3 ( x = 0.0, 0.05, 0.1, 0.3, 0.5 and 0.8) have been prepared from citrate precursors and characterized by XRD, TPR, TG-H2 and XPS. Catalytic experiments in the reforming of CH4 with CO2 have been carried out in a tubular reactor at 750 °C and atmospheric pressure. After the catalytic tests the catalysts were studied by TPO and SEM. Partial substitution of La by Ca was performed to stabilize Ni particles and to prevent carbon deposition. The XRD profiles showed that the perovskite structure is the only compound identified within the 0 ≤ x ≤ 0.05 range, whereas for x ≥ 0.1 compounds such as spinel-type La2NiO4, NiO and CaO were observed in addition to the perovskite oxide. On the other hand, segregation of NiO, even in the unsubstituted perovskite ( x = 0.0), was confirmed by TPR and XPS. The catalytic tests showed that the replacement of La by Ca, which has a lower ionic radius, favored a higher activity and stronger resistance to carbon deposition. However, this coking resistance depended on the Ca-loading and the catalysts with x = 0.05 and x = 0.8 were the most stable against deactivation. [ABSTRACT FROM AUTHOR]
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- 2008
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18. Some Key Factors in Emulsion Polymerization Process Development.
- Author
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Meuldijk, J., Kemmere, M., de Lima, S., Reynhout, X., Drinkenburg, A., and German, A.
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POLYMERIZATION ,CHEMICAL reactions ,CHEMICALS ,CHEMICAL processes ,EMULSIONS - Abstract
Emulsion polymerisations are mostly carried out as (semi-)batch processes in stirred tanks. In ab-initio emulsion polymerisation the product properties in terms of particle concentration and particle size distribution strongly depend on the course of the nucleation stage. The course of the nucleation stage is strongly related to the quality of emulsification and the temperature of the reaction mixture. The influence of temperature on the particle size distribution was investigated for completely isothermal as well as for non-isothermal operation. Reaction calorimetry has been chosen as a tool to define an operating window for control of the nucleation stage in emulsion polymerisation. It was demonstrated that reaction calorimetry is a very poweful tool to define operational details for complete control of the nucleation process in emulsion polymerisation in stirred tanks accurately and quickly. Colloidal stability of the latex is an important issue during the stage of particle growth by simultaneous polymerisation and monomer absorption from the monomer droplets. It was demonstrated that a proper recipe for the production of a colloidally stable latex can be developed in a short time with reaction calorimetry. [ABSTRACT FROM AUTHOR]
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- 2003
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19. Rheology of THF hydrate slurries at high pressure
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de Lima Silva Paulo H., Naccache Mônica F., de Souza Mendes Paulo R., Teixeira Adriana, and Valim Leandro S.
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Chemical technology ,TP1-1185 ,Energy industries. Energy policy. Fuel trade ,HD9502-9502.5 - Abstract
One of the main issues in the area of drilling and production in deep and ultra-deep water in the oil industry is the formation of natural gas hydrates. Hydrates are crystalline structures resembling ice, which are usually formed in conditions of high pressure and low temperature. Once these structures are formed, they can grow and agglomerate, forming plugs that can eventually completely or partially block the production lines, causing huge financial losses. To predict flow behavior of these fluids inside the production lines, it is necessary to understand their mechanical behavior. This work analyzes the rheological behavior of hydrates slurries formed by a mixture of water and Tetrahydrofuran (THF) under high pressure and low temperature conditions, close to the ones found in deep water oil exploration. The THF hydrates form similar structures as the hydrates originally formed in the water-in-oil emulsions in the presence of natural gas, at extreme conditions of high pressure and low temperature. The experiments revealed some important issues that need to be taken into account in the rheological measurements. The results obtained show that the hydrate slurry viscosity increases with pressure. Oscillatory tests showed that elasticity and yield stress also increase with pressure.
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- 2020
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20. Ascorbic acid for management of oral surgery pain not responding to conventional medication: case report
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Arabadzhiev Ivan Hristov, Maurer Peter, and de Lima Stevao Eber Luis
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ascorbic acid ,pain ,oral surgery ,Dentistry ,RK1-715 ,Surgery ,RD1-811 - Abstract
Introduction: A 20-year-old female who experienced severe pain during six weeks after dental treatment was followed by oral surgeries. This article focuses on the usage of high dose of oral L-ascorbic acid for pain alleviation which did not respond to conventional pain medication. Observation: A female patient complaining about severe pain in tooth number 31 which was measured at every visit she made to the office, using Numerical Rating Pain Scale (NRPS) as the model for registration. Comments: Implementation of different types of analgesics along with laser-therapy were not able to reduce patient's ache during forty four consecutive days. Pain relief was only obtained at the next day with L-ascorbic acid supplementation to patient's previous medication. A complete absence of pain was reported by the patient on the seventh day after the ascorbate intake. Conclusion: Given to its action of mediating a variety of essential biological and biochemical functions, benefits, low cost, and safety, L-ascorbic acid could be considered by general dentists and oral surgeons as possible pain modulator after oral/dental surgical procedures.
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- 2020
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21. The vernacular press and the emergence of modern Indonesian consciousness (1855-1913) A. B. Adam
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DE LIMA, S. D. G.
- Published
- 1987
22. SERF.
- Author
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Rundensteiner, E. A., Claypool, K., Li, M., Chen, L., Zhang, Z., Natarajan, C., Jin, J., De Lima, S., and Weiner, S.
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- 1999
- Full Text
- View/download PDF
23. Postmenopausal Osteoporosis reference genes for qPCR expression assays
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Sergio Crovella, Jaqueline de Azevêdo Silva, Will de Barros Pita, Alexandre Domingues Barbosa, Camilla Albertina Dantas de Lima, Suelen Cristina de Lima, Paula Sandrin-Garcia, de Lima, C. A. D., de Lima, S. C., Barbosa, A. D., Sandrin-Garcia, P., de Barros Pita, W., de Azevedo Silva, J., and Crovella, S.
- Subjects
lcsh:Medicine ,Computational biology ,Postmenopausal osteoporosis ,Biology ,Real-Time Polymerase Chain Reaction ,Article ,law.invention ,qPCR ,osteoporosis ,law ,Reference genes ,Gene expression ,Genetics ,Humans ,Genetic Predisposition to Disease ,lcsh:Science ,Gene ,Osteoporosis, Postmenopausal ,Polymerase chain reaction ,Aged ,Aged, 80 and over ,Regulation of gene expression ,Multidisciplinary ,Gene Expression Profiling ,lcsh:R ,Computational Biology ,Reproducibility of Results ,Gene expression profiling ,Real-time polymerase chain reaction ,Gene Expression Regulation ,Female ,lcsh:Q ,Biomarkers - Abstract
Osteoporosis (OP) is a multifactorial disease influenced by genetic factors in more than half of the cases. In spite of the efforts to clarify the relationship among genetic factors and susceptibility to develop OP, many genetic associations need to be further functionally validated. Besides, some limitations as the choice of stably expressed reference genes (RG) should be overcome to ensure the quality and reproducibility of gene expression assays. To our knowledge, a validation study for RG in OP is still missing. We compared the expression levels, using polymerase chain reaction quantitative real time (qPCR) of 10 RG (G6PD, B2M, GUSB, HSP90, EF1A, RPLP0, GAPDH, ACTB, 18 S and HPRT1) to assess their suitability in OP analysis by using GeNorm, Normfinder, BestKeeper and RefFinder programs. A minimal number of two RG was recommended by GeNorm to obtain a reliable normalization. RPLP0 and B2M were identified as the most stable genes in OP studies while ACTB, 18 S and HPRT1 were inadequate for normalization in our data set. Moreover, we showed the dramatic effects of suboptimal RG choice on the quantification of a target gene, highlighting the importance in the identification of the most appropriate reference gene to specific diseases. We suggest the use of RPLP0 and B2M as the most stable reference genes while we do not recommend the use of the least stable reference genes HPRT1, 18 S and ACTB in OP expression assays using PBMC as biological source. Additionally, we emphasize the importance of individualized and careful choice in software and reference genes selection.
- Published
- 2019
24. OPTICAL MICROSCOPY APPLIED TO THE DETERMINATION OF Cu–Al–Be SHAPE MEMORY ALLOYS PHASE TRANSFORMATION TEMPERATURES.
- Author
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de Azevedo Melo, T. A., Gomes, R. Medeiros, da Silva Júnior, M. Q., de Lima, S. J. Guedes, and Brito, I. C. Andrade
- Subjects
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ALLOY analysis , *POLYCRYSTALS , *SHAPE memory alloys , *PHASE transitions , *MICROSCOPY , *CALORIMETRY - Abstract
Polycrystalline specimens of Cu-Al-Be shape memory alloys were homogenized at 1123K during 12h and water-quenched at room temperature. The phase transformation temperatures were determined via optical microscopy technique, using a cooling/heating device attached to the microscope stage. Comparisons have been made with data obtained via differential scanning calorimetry. The data show that the former technique can be successfully applied to the determination of typical transition temperatures occurring in shape memory alloys. As far as sample related problems are concerned, the former technique can also represent an advantageous alternative over the latter given the fact that the analyses can be performed in more representative area. The Mf and As temperatures precise detection depend on the chosen resolution of the optical microscope. [ABSTRACT FROM AUTHOR]
- Published
- 2010
25. Hydroxychloroquine is associated with lower seroconversion upon 17DD-Yellow fever primovaccination in patients with primary Sjögren's syndrome.
- Author
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Libardi Lira Machado KL, da Costa-Rocha IA, Gonçalves Rodrigues Aguiar L, Ribeiro Moulaz I, Tatiyama Miyamoto S, Costa Martins P, Vieira Serrano E, Espíndula Gianordoli AP, da Penha Gomes Gouvea M, de Fatima Bissoli M, Maria Barbosa de Lima S, Dias Schwarcz W, de Souza Azevedo A, Fernandes Amorim da Silva J, Tourinho Santos R, Pedro Brito-de-Sousa J, Coelho-Dos-Reis JG, Campi-Azevedo AC, Teixeira-Carvalho A, Peruhype-Magalhães V, Fontana Sutile Tardetti Fantinato F, Maria Henrique da Mota L, Assis Martins-Filho O, and Valim V
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- Humans, Female, Middle Aged, Male, Adult, Yellow Fever Vaccine immunology, Aged, Viremia drug therapy, Viremia immunology, Yellow fever virus immunology, Cytokines blood, Biomarkers blood, Hydroxychloroquine therapeutic use, Sjogren's Syndrome drug therapy, Sjogren's Syndrome immunology, Seroconversion, Yellow Fever immunology, Yellow Fever prevention & control, Antibodies, Viral blood, Antibodies, Neutralizing blood
- Abstract
The present study aimed at investigating whether the hydroxychloroquine (HCQ) treatment would impact the neutralizing antibody production, viremia levels and the kinetics of serum soluble mediators upon planned 17DD-Yellow Fever (YF) primovaccination (Bio-Manguinhos-FIOCRUZ) of primary Sjögren's syndrome (pSS). A total of 34 pSS patients and 23 healthy controls (HC) were enrolled. The pSS group was further categorized according to the use of HCQ (HCQ and Non-HCQ). The YF-plaque reduction neutralization test (PRNT ≥1:50), YF viremia (RNAnemia) and serum biomarkers analyses were performed at baseline and subsequent time-points (Day0/Day3-4/Day5-6/Day7/Day14-D28). The pSS group showed PRNT titers and seropositivity rates similar to those observed for HC (GeoMean = 238 vs 440, p = .11; 82% vs 96%, p = .13). However, the HCQ subgroup exhibited lower seroconversion rates as compared to HC (GeoMean = 161 vs 440, p = .04; 69% vs 96%, p = .02) and Non-HQC (GeoMean = 161 vs 337, p = .582; 69% vs 94%, p = .049). No differences in YF viremia were observed amongst subgroups. Serum biomarkers analyses demonstrated that HCQ subgroup exhibited increased levels of CCL2, CXL10, IL-6, IFN-γ, IL1-Ra, IL-9, IL-10, and IL-2 at baseline and displayed a consistent increase of several biomarkers along the kinetics timeline up to D14-28. These results indicated that HCQ subgroup exhibited a deficiency in assembling YF-specific immune response elicited by 17DD-YF primovaccination as compared to Non-HCQ subgroup. Our findings suggested that hydroxychloroquine is associated with a decrease in the humoral immune response after 17DD-YF primovaccination.
- Published
- 2024
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26. Yellow fever neutralizing antibody seroprevalence proportion and titers in previously vaccinated adults with chronic kidney disease.
- Author
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Cristina Martini Rodrigues C, Caroline Ribeiro Sales A, Marli Christovam Sartori A, de Souza Azevedo A, Maria Barbosa de Lima S, de Melo Picone C, Keiko Sato P, Nazareth Lara A, Takesaki Miyaji K, Sérgio Azevedo L, Caldin B, Camera Pierrotti L, and Heloisa Lopes M
- Subjects
- Adult, Humans, Antibodies, Neutralizing, Cross-Sectional Studies, Seroepidemiologic Studies, Antibodies, Viral, Yellow fever virus, Vaccination, Yellow Fever prevention & control, Yellow Fever Vaccine, Renal Insufficiency, Chronic complications, Renal Insufficiency, Chronic therapy
- Abstract
Studies on yellow fever vaccine (YF) in chronic kidney disease (CKD) patients are scarce. This cross-sectional study aimed to evaluate YF neutralizing antibody seroprevalence and titers in previously vaccinated adults with CKD, on dialysis (D-CKD) or not (ND-CKD), compared to healthy persons. The micro Plaque Reduction Neutralization-Horseradish Peroxidase (μPRN-HP) test was used. Antibody titers were expressed as the reciprocal of the highest dilution that neutralized the challenge virus by 50 % (μPRN50). Seropositivity cut-off was set at ≥ 1:100. We included 153 participants: 46 ND-CKD, 50 D-CKD and 57 healthy adults. Median ages were 58.3, 55 and 52.2 years, respectively. Median time since YF vaccination was 22.3, 18.5 and 48.3 months respectively. There were no statistically significant differences in YF seroprevalence and neutralizing antibodies titers among groups: 100 % of ND-CKD; 96 % of D-CKD and 100 % of healthy participants were seropositive. Geometric mean titers (GMT) were 818.5, 683.0 and 665.5, respectively (p = 0.289)., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Marta H Lopes reports financial support was provided by State of Sao Paulo Research Foundation., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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27. Editorial: Promoting nervous system regeneration by treatments targeting neuron-glia interactions.
- Author
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De Lima S, Mietto BS, Ribas VT, Ribeiro-Resende VT, Oliveira ALR, and Park KK
- Abstract
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
- Published
- 2024
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28. Biostimulants in Corn Cultivation as a Means to Alleviate the Impacts of Irregular Water Regimes Induced by Climate Change.
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Luiz Piati G, Ferreira de Lima S, Lustosa Sobrinho R, Dos Santos OF, Vendruscolo EP, Jacinto de Oliveira J, do Nascimento de Araújo TA, Mubarak Alwutayd K, Finatto T, and AbdElgawad H
- Abstract
Climate change alters regular weather seasonality. Corn is one of the main crops affected by irregular water regimes. Due to complications in decision-making processes related to climate change, it is estimated that planting corn outside the optimal window results in around USD 340 million in losses per year in the United States' Corn Belt. In turn, exogenous plant growth regulators have been gaining prominence due to their potential to positively influence the morphology and physiology of plants under stress. This study was based on the hypothesis that the use of plant growth regulators can assist in mitigating the adverse effects of climate change on corn plants sown both inside and outside the recommended planting period. In this context, the effects of biostimulant application on gas exchange in corn plants sown within and outside the recommended period were evaluated. The experiment was carried out in randomized blocks in a 4 × 5 × 2 factorial scheme with four repetitions. These were four sowing times, the application of the biostimulants via seeds in five doses, and foliar applications (presence and absence). The biostimulant doses were 0.00, 6.25, 12.50, 18.75, and 25 mL kg
-1 . The foliar application used a dose of 500 mL ha-1 . Only in the period (2017/2) higher doses of biostimulants indicated a decrease in the water use efficiency of plants, suggesting the need to evaluate this variable carefully. In this regard, future studies may investigate the ideal doses and application timings of biostimulants for different edaphoclimatic conditions. In general, the combined use of biostimulants on seeds and as a foliar treatment boosted physiological activity and stimulated photosynthetic processes in corn plants. Based on these data, plant regulators can be a useful tool to mitigate the adverse effects of climate change on corn plants sown inside and outside the planting period.- Published
- 2023
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29. Introducing the Canadian Urology Student Interest Group (CUSIG) Initial experience from a national webinar.
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Bouhadana D, de Lima S, Nguyen DD, Bhojani N, Lee JY, Metcalfe P, MacLellan DL, and Domes T
- Published
- 2023
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30. Treatment of Unresectable Cutaneous Squamous Cell Carcinoma with Cemiplimab in a Patient on Dialysis.
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Grace de Lima S, Hyrcza MD, and Monzon JG
- Abstract
Program death-1 inhibitors, a class of immune-checkpoint inhibitors, are now the standard of care in a variety of cancer settings, including cutaneous malignancies, such as melanomas, Merkel cell, and cutaneous squamous cell carcinomas (cSCCs). The clinical trials that led to the approval of the programmed death-1 inhibitor cemiplimab-rwlc (Libtayo
® ) for use in advanced cSCC excluded patients with autoimmune disease and those that required systemic immunosuppressive treatments, or had undergone solid-organ transplantation. Also, to be eligible, patients had to have adequate organ function. Here, we present the first report of a patient that has been successfully treated with cemiplimab for locally advanced cSCC while simultaneously on dialysis for treatment of renal failure following renal transplant., Competing Interests: Dr. J. Monzon sits on advisory boards for BMS, Pfizer, Sanofi, and Taiho and has a grant funding from Merck., (© 2023 The Author(s). Published by S. Karger AG, Basel.)- Published
- 2023
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31. Lung Inflammasome Activation in SARS-CoV-2 Post-Mortem Biopsies.
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Baena Carstens L, Campos D'amico R, Fernandes de Moura K, Morais de Castro E, Centenaro F, Silva Barbosa G, Vieira Cavalcante da Silva G, Brenny I, Honório D'Agostini JC, Hlatchuk EC, Pissette de Lima S, Camargo Martins AP, De Castro Deus M, Konzen Klein C, Kubaski Benevides AP, Nagashima S, Machado-Souza C, Pinho RA, Pellegrino Baena C, and de Noronha L
- Subjects
- Humans, Inflammasomes metabolism, SARS-CoV-2, Interleukin-18, NF-kappa B metabolism, Angiotensin-Converting Enzyme 2, Autopsy, Caspase 1 metabolism, Lung metabolism, Cytokines metabolism, Biopsy, NLR Family, Pyrin Domain-Containing 3 Protein metabolism, COVID-19, Influenza A Virus, H1N1 Subtype metabolism
- Abstract
The inflammasome complex is a key part of chronic diseases and acute infections, being responsible for cytokine release and cell death mechanism regulation. The SARS-CoV-2 infection is characterized by a dysregulated cytokine release. In this context, the inflammasome complex analysis within SARS-CoV-2 infection may prove beneficial to understand the disease’s mechanisms. Post-mortem minimally invasive autopsies were performed in patients who died from COVID-19 (n = 24), and lung samples were compared to a patient control group (n = 11) and an Influenza A virus H1N1 subtype group from the 2009 pandemics (n = 10). Histological analysis was performed using hematoxylin-eosin staining. Immunohistochemical (IHC) staining was performed using monoclonal antibodies against targets: ACE2, TLR4, NF-κB, NLRP-3 (or NALP), IL-1β, IL-18, ASC, CASP1, CASP9, GSDMD, NOX4, TNF-α. Data obtained from digital analysis underwent appropriate statistical tests. IHC analysis showed biomarkers that indicate inflammasome activation (ACE2; NF-κB; NOX4; ASC) were significantly increased in the COVID-19 group (p < 0.05 for all) and biomarkers that indicate cell pyroptosis and inflammasome derived cytokines such as IL-18 (p < 0.005) and CASP1 were greatly increased (p < 0.0001) even when compared to the H1N1 group. We propose that the SARS-CoV-2 pathogenesis is connected to the inflammasome complex activation. Further studies are still warranted to elucidate the pathophysiology of the disease., Competing Interests: The authors declare no conflict of interest.
- Published
- 2022
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32. Rupture and chemical accumulation in contact lenses with dexamethasone eye drop administration after congenital cataract surgery.
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De Lima S, Erdal N, Adolfsson K, Hakkarainen M, and Kugelberg M
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- Child, Dexamethasone, Humans, Ophthalmic Solutions, Cataract, Contact Lenses, Lens, Crystalline
- Abstract
Purpose: To investigate whether contact lenses used after surgery for congenital cataracts act as a depot for dexamethasone, which would allow the prescribed amount of drops to be reduced, and to examine whether the preservative benzalkonium chloride accumulates in the contact lens matrix, which would suggest a need for more frequent replacements., Methods: Contact lenses (n = 10) worn by infants treated with dexamethasone eye drops after congenital cataract surgery were analysed with scanning electron microscopy, UV-vis,
1 H-NMR and LDI-MS for chemical deposits and for changes on the contact lens surface. Unused lenses (n = 5) and lenses (n = 4) from patients with no eye drop treatment were analysed as reference., Results: The treated contact lenses displayed ruptured surfaces in comparison with unused and reference lenses. Dexamethasone and BAK were not detected in any of the lenses. A polyethylene oxide component was found in the treated lenses, likely originating from the dexamethasone eye drops or the contact lens solution., Conclusion: Dexamethasone and BAK do not accumulate in the contact lenses, and a depot effect of any clinical significance is unlikely. Therefore, the number of drops given after surgery should remain the same regardless of whether the child has contact lenses. The ruptured surface may both decrease the child's comfort and increase the risk of microbial adhesion, and so it is recommended that contact lenses should be replaced once a month throughout the course of anti-inflammatory eye drop treatment after surgery for congenital cataract., (© 2021 The Authors. Acta Ophthalmologica published by John Wiley & Sons Ltd on behalf of Acta Ophthalmologica Scandinavica Foundation.)- Published
- 2022
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33. Kinematic Loggers-Development of Rugged Sensors and Recovery Systems for Field Measurements of Stone Rolling Dynamics and Impact Accelerations during Floods.
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Biggs H, Starr A, Smith B, de Lima S, Sykes J, Haddadchi A, Smart G, and Hicks M
- Subjects
- Acceleration, Biomechanical Phenomena, Software, Floods, Rivers
- Abstract
Discrete particle dynamics is one of the least understood aspects of river bedload transport, but in situ measurement of stone movement during floods poses a significant technical challenge. A promising approach to address this knowledge gap is to use sensors embedded within stones. Sensors must be waterproof and recoverable after being transported downstream and potentially buried by other sediment. To address this challenge rugged sensors (Kinematic Loggers) were developed for deployment inside stones (ranging in size from cobbles to boulders) during floods. The sensors feature a 9-axis inertial measurement unit, 3-axis high-g accelerometer, 128 MB flash memory, and a 433 MHz LoRa radio transmission module for sensor recovery. The sensors are enclosed in rugged waterproof housings for deployment in extreme conditions (i.e., bedload transport during floods). Novel relay units and drone-based recovery systems were also developed for finding the sensors after field deployments. Firmware to control the sensors and relay units was developed, as well as software for configuring the sensors and an android application for communicating with the sensors via the LoRa radio transmission module. This paper covers the technical development of the sensors, mounting them inside stones, and field recovery tests. Although designed for measurement of coarse bedload transport and particle dynamics during floods, the sensors are equally applicable for deployment in other harsh environments, such as to study landslide and rockfall dynamics.
- Published
- 2022
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34. Retinal Ganglion Cell Axon Regeneration Requires Complement and Myeloid Cell Activity within the Optic Nerve.
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Peterson SL, Li Y, Sun CJ, Wong KA, Leung KS, de Lima S, Hanovice NJ, Yuki K, Stevens B, and Benowitz LI
- Subjects
- Animals, Axons immunology, Complement C1q immunology, Complement C3 immunology, Female, Male, Mice, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Knockout, Myeloid Cells immunology, Nerve Regeneration physiology, Optic Nerve Injuries immunology, Optic Nerve Injuries pathology, Retinal Ganglion Cells immunology, Axons metabolism, Complement C1q metabolism, Complement C3 metabolism, Myeloid Cells metabolism, Optic Nerve Injuries metabolism, Retinal Ganglion Cells metabolism
- Abstract
Axon regenerative failure in the mature CNS contributes to functional deficits following many traumatic injuries, ischemic injuries, and neurodegenerative diseases. The complement cascade of the innate immune system responds to pathogen threat through inflammatory cell activation, pathogen opsonization, and pathogen lysis, and complement is also involved in CNS development, neuroplasticity, injury, and disease. Here, we investigated the involvement of the classical complement cascade and microglia/monocytes in CNS repair using the mouse optic nerve injury (ONI) model, in which axons arising from retinal ganglion cells (RGCs) are disrupted. We report that central complement C3 protein and mRNA, classical complement C1q protein and mRNA, and microglia/monocyte phagocytic complement receptor CR3 all increase in response to ONI, especially within the optic nerve itself. Importantly, genetic deletion of C1q , C3 , or CR3 attenuates RGC axon regeneration induced by several distinct methods, with minimal effects on RGC survival. Local injections of C1q function-blocking antibody revealed that complement acts primarily within the optic nerve, not retina, to support regeneration. Moreover, C1q opsonizes and CR3
+ microglia/monocytes phagocytose growth-inhibitory myelin debris after ONI, a likely mechanism through which complement and myeloid cells support axon regeneration. Collectively, these results indicate that local optic nerve complement-myeloid phagocytic signaling is required for CNS axon regrowth, emphasizing the axonal compartment and highlighting a beneficial neuroimmune role for complement and microglia/monocytes in CNS repair. SIGNIFICANCE STATEMENT Despite the importance of achieving axon regeneration after CNS injury and the inevitability of inflammation after such injury, the contributions of complement and microglia to CNS axon regeneration are largely unknown. Whereas inflammation is commonly thought to exacerbate the effects of CNS injury, we find that complement proteins C1q and C3 and microglia/monocyte phagocytic complement receptor CR3 are each required for retinal ganglion cell axon regeneration through the injured mouse optic nerve. Also, whereas studies of optic nerve regeneration generally focus on the retina, we show that the regeneration-relevant role of complement and microglia/monocytes likely involves myelin phagocytosis within the optic nerve. Thus, our results point to the importance of the innate immune response for CNS repair., (Copyright © 2021 the authors.)- Published
- 2021
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35. Apical hypertrophic cardiomyopathy: A case series at a Brazilian referral center with a maximal follow-up of 15 years.
- Author
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Chaves AVF, Soares Bezerra L, Oliveira Santos-Veloso MA, Calado EB, Markman M, Bezerra de Melo da Silveira Lordsleem A, de Lima SG, and Markman-Filho B
- Subjects
- Death, Sudden, Cardiac etiology, Death, Sudden, Cardiac prevention & control, Follow-Up Studies, Humans, Middle Aged, Referral and Consultation, Retrospective Studies, Risk Assessment, Risk Factors, Cardiomyopathy, Hypertrophic diagnosis, Cardiomyopathy, Hypertrophic therapy, Defibrillators, Implantable
- Abstract
Background: Apical hypertrophic cardiomyopathy (AHCM) is a rare cardiomyopathy, in which hypertrophy occurs predominantly in the ventricular apex, and in some cases with a high risk of sudden cardiac death., Objective: The aim of this paper is to present a case series of patients with AHCM and describe their main clinical, echocardiographic and electrocardiographic characteristics, the recommendation for an implantable cardioverter-defibrillator (ICD) and the frequency of sudden cardiac death (SCD)., Methods: A retrospective case series was conducted at the referral center of a federal teaching hospital, between the years 2005 to 2020, involving patients with an echocardiographic diagnosis of AHCM. The parameters of the American College of Cardiology and the European Society of Cardiology were used to assess the risk of SCD., Results: A total of 11 individuals were assessed with a mean age of 55.3 years, mean follow-up of 41.2 months, most of whom were symptomatic at diagnosis (72.7%). The most frequent symptom was dyspnea (27.3%). A family history of SCD was described in 45.5% of cases. Due to a high risk of SCD, four patients received ICDs. One patient presented sudden cardiac death after having refused the ICD., Conclusions: Symptoms and alterations in the imaging exams are significant factors in the clinical and prognostic assessment of patients with AHCM., (Copyright © 2021 Elsevier Masson SAS. All rights reserved.)
- Published
- 2021
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36. Swedish Parents' Experiences and Their Need for Support When Having a Child with Congenital Cataract: A Qualitative Study.
- Author
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De Lima S, Kugelberg M, and Jirwe M
- Subjects
- Child, Female, Humans, Parents, Pregnancy, Qualitative Research, Sweden, Cataract diagnosis, Family
- Abstract
Purpose: To explore parents' experiences of living with a child with congenital cataract, with the intention of identifying how to improve the parental support., Design and Method: A qualitative descriptive design using semi-structured interviews. The parents were interviewed when the children were aged 12-24 months. All children were operated on for congenital cataract before three months of age. The interviews were transcribed and analysed using qualitative content analysis with an inductive approach. Findings were reported following the Standard for Reporting Qualitative Research (SRQR) checklist., Results: Three categories emerged from the data: trying to survive during a chaotic time; adapting to a different normal; being in need of support. When receiving the preliminary diagnosis, most of the parents were upset but managed well once the initial shock had subsided. However, some described feelings of despair, difficulties in accepting the situation and in connecting with the child. All parents stated that, to be valuable, a counsellor needed to have insights in how the eye works and the function of visual development., Conclusion: The parents' need for psychosocial support in the early post-diagnostic stage varied greatly. Early identification of those in need of specialized counselling is therefore of importance, preferably at the maternity ward or by the regional ophthalmologist when the referral is made., Clinical Implications: The study provides understanding of the importance to take the parents' well-being beyond the medical issues into consideration. This knowledge can be used to provide support at an earlier stage in the treatment programme than is currently the case., Competing Interests: Declaration of competing interest None of the authors has a proprietary or financial interest in any material, method, or product mentioned in this article., (Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Published
- 2021
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37. Applications of a UV optical nitrate sensor in a surface water/groundwater quality field study.
- Author
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Burbery L, Abraham P, Wood D, and de Lima S
- Subjects
- Agriculture, Environmental Monitoring, Nitrates analysis, Water, Groundwater, Water Pollutants, Chemical analysis
- Abstract
Examples of the utility of UV optical nitrate sensors are provided for two field applications, investigating nitrate pollution in a lowland, peri-urban catchment. In one application, rapid, in-stream longitudinal nitrate surveys were made in summer and winter, by fixing an optical nitrate sensor operating in continuous measurement mode to a kayak that was paddled along 10 km of the mainstem of the low-order stream in under 4 h. Nitrate concentrations ranged between 3.45 and 6.39 mg NO
3 -N/L. Nitrate hot-spots and cool-spots were mapped and found to relate to point discharges from spring-fed tributaries and land drains. Effective nitrate removal (dN/dx = - 0.08 mg N/L/km), inferred to be from assimilation reactions, was evident in the summer dataset, but not the winter nitrate dataset. In a second application, the optical sensor was configured with appropriate technology to establish an autonomous and fully automated nitrate monitoring station. The station makes daily nitrate measurements of surface water, and groundwater, sampled from a cluster of four multi-level wells. Quarterly maintenance of the nitrate sensor has proven sufficient to keep measurement errors under 5%. Most nitrate variation has been recorded at or near the water table where concentrations have ranged between 3.47 and 5.88 mg NO3 -N/L, and annual maxima have occurred in late winter/spring, which coincides with when most nitrate leaching occurs from agricultural land. Seasonal nitrate patterns are not evident in groundwater sampled from 8-m depth, or deeper. High-frequency monitoring has revealed that some infra-season, short-term variability also occurs in shallow groundwater nitrate, driven by storm events, and which on occasion results in a temporary inversion of the groundwater nitrate-depth profile.- Published
- 2021
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38. Six months under uncontrolled relative humidity and room temperature changes technological characteristics and maintains the physicochemical and functional properties of carioca beans (Phaseolus vulgaris L.).
- Author
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E G Alves N, J C Gomes M, M Vasconcelos C, C Lima A, L S de Lima S, S Brito E, Z Bassinello P, and S D Martino H
- Subjects
- Catechol Oxidase metabolism, Dietary Fiber analysis, Hardness, Humans, Humidity, Hydrogen-Ion Concentration, Kaempferols analysis, Kaempferols chemistry, Nutrients analysis, Peroxidases metabolism, Phaseolus metabolism, Phytic Acid analysis, Spectrophotometry, Temperature, Time Factors, Food Storage methods, Phaseolus chemistry
- Abstract
Carioca beans contribute to health maintenance around the world, and the evaluation of commercial postharvest storage (CPS) ensures their quality. This study aimed to evaluate the effect of CPS on technological, physicochemical and functional properties of carioca beans. Two genotypes (Pontal-PO and Madreperola-MP beans) were stored under CPS or controlled conditions and were evaluated after harvest and after three- and six-months storage. PO and MP hardened with time, but the cooking time did not differ. PO is darker than MP and both darkened over time. Storage time affected pH and acidity of the beans and MP presented better physicochemical properties than PO, with lower activity of peroxidase (p = 0.004) and polyphenoloxidase (p = 0.001) enzymes. Glycosylated kaempferol was suggested as a possible chemical marker to differentiate the aging of PO and MP beans. In conclusion, besides the technological differences, the storage was able to prevent physicochemical and functional alterations of beans., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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39. Efficacy of Multi-Layered Soft Silicone: A Randomized Clinical Trial.
- Author
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Mendonça Moraes C, Soares RSA, Eberhardt TD, Silveira LBTD, Alves PJP, and Beatriz Soares de Lima S
- Subjects
- Silicones
- Published
- 2020
- Full Text
- View/download PDF
40. Congenital cataract in newborns: A qualitative study on parents' experiences of the surgery and subsequent care.
- Author
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De Lima S, Kugelberg M, and Jirwe M
- Subjects
- Cataract diagnosis, Cataract urine, Cataract Extraction psychology, Child, Preschool, Female, Humans, Infant, Information Seeking Behavior, Male, Postoperative Period, Professional-Family Relations, Qualitative Research, Sweden, Cataract congenital, Parents psychology, Stress, Psychological psychology
- Abstract
Purpose: Having a child with congenital cataract that requires surgery, contact lens treatment, and frequent medication is a life-altering experience. The aim of this study was to provide more in-depth knowledge of parents' experiences of diagnosis, surgery, and subsequent care, in order to find the areas for improvement., Methods: Data were collected via semi-structured interviews with parents recruited from St. Erik Eye Hospital, Sweden. The children were operated for congenital cataract before 3 months of age and were aged 12-24 months at the time of the interviews. The interviews were analysed using qualitative content analysis with an inductive approach., Results: Three themes emerged from the data: living in a turbulent time before the diagnosis, coping during the time of initial treatment, and managing the responsibility of their child's visual development. All parents expressed confidence in the operating unit. However, there was a clear need for reliable information on the condition, the surgery, and likely the long-term outcome in the time between receiving the preliminary diagnosis at the maternity ward and getting it confirmed by a paediatric ophthalmologist., Conclusion: Much of the parents' concern in this turbulent time is linked to poor information before the visit to the eye hospital. We, therefore, recommend earlier contact with the eye hospital, preferably at the time of writing the referral. Moreover, the possibility of providing up-to-date information via alternative information channels such as smartphone apps should be investigated., (© 2020 The Authors. Acta Ophthalmologica published by John Wiley & Sons Ltd on behalf of Acta Ophthalmologica Scandinavica Foundation.)
- Published
- 2020
- Full Text
- View/download PDF
41. Survey on Non-Human Primates and Mosquitoes Does not Provide Evidences of Spillover/Spillback between the Urban and Sylvatic Cycles of Yellow Fever and Zika Viruses Following Severe Outbreaks in Southeast Brazil.
- Author
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Abreu FVS, Ferreira-de-Brito A, Azevedo AS, Linhares JHR, de Oliveira Santos V, Hime Miranda E, Neves MSAS, Yousfi L, Ribeiro IP, Santos AACD, Dos Santos E, Santos TPD, Teixeira DS, Gomes MQ, Fernandes CB, Silva AMVD, Lima MDRQ, Paupy C, Romano APM, Ano Bom APD, Oliveira-Pinto LM, Moutailler S, Motta MA, Castro MG, Bonaldo MC, Maria Barbosa de Lima S, and Lourenço-de-Oliveira R
- Subjects
- Animals, Brazil epidemiology, Genome, Viral, Genotype, Mosquito Vectors virology, Primates virology, Yellow Fever epidemiology, Yellow fever virus, Zika Virus, Zika Virus Infection epidemiology, Disease Outbreaks, Yellow Fever transmission, Yellow Fever virology, Zika Virus Infection transmission, Zika Virus Infection virology
- Abstract
In the last decade, Flaviviruses such as yellow fever (YFV) and Zika (ZIKV) have expanded their transmission areas. These viruses originated in Africa, where they exhibit both sylvatic and interhuman transmission cycles. In Brazil, the risk of YFV urbanization has grown, with the sylvatic transmission approaching the most densely populated metropolis, while concern about ZIKV spillback to a sylvatic cycle has risen. To investigate these health threats, we carried out extensive collections and arbovirus screening of 144 free-living, non-human primates (NHPs) and 5219 mosquitoes before, during, and after ZIKV and YFV outbreaks (2015-2018) in southeast Brazil. ZIKV infection was not detected in any NHP collected at any time. In contrast, current and previous YFV infections were detected in NHPs sampled between 2017 and 2018, but not before the onset of the YFV outbreak. Mosquito pools screened by high-throughput PCR were positive for YFV when captured in the wild and during the YFV outbreak, but were negative for 94 other arboviruses, including ZIKV, regardless of the time of collection. In conclusion, there was no evidence of YFV transmission in coastal southeast Brazil before the current outbreak, nor the spread or establishment of an independent sylvatic cycle of ZIKV or urban Aedes aegypti transmission of YFV in the region. In view of the region's receptivity and vulnerability to arbovirus transmission, surveillance of NHPs and mosquitoes should be strengthened and continuous.
- Published
- 2020
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42. Zika Virus Surveillance at the Human-Animal Interface in West-Central Brazil, 2017-2018.
- Author
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Pauvolid-Corrêa A, Gonçalves Dias H, Marina Siqueira Maia L, Porfírio G, Oliveira Morgado T, Sabino-Santos G, Helena Santa Rita P, Teixeira Gomes Barreto W, Carvalho de Macedo G, Marinho Torres J, Arruda Gimenes Nantes W, Martins Santos F, Oliveira de Assis W, Castro Rucco A, Mamoru Dos Santos Yui R, Bosco Vilela Campos J, Rodrigues Leandro E Silva R, da Silva Ferreira R, Aparecido da Silva Neves N, Charlles de Souza Costa M, Ramos Martins L, Marques de Souza E, Dos Santos Carvalho M, Gonçalves Lima M, de Cássia Gonçalves Alves F, Humberto Guimarães Riquelme-Junior L, Luiz Batista Figueiró L, Fernandes Gomes de Santana M, Gustavo Rodrigues Oliveira Santos L, Serra Medeiros S, Lopes Seino L, Hime Miranda E, Henrique Rezende Linhares J, de Oliveira Santos V, Almeida da Silva S, Araújo Lúcio K, Silva Gomes V, de Araújo Oliveira A, Dos Santos Silva J, de Almeida Marques W, Schafer Marques M, Junior França de Barros J, Campos L, Couto-Lima D, Coutinho Netto C, Strüssmann C, Panella N, Hannon E, Cristina de Macedo B, Ramos de Almeida J, Ramos Ribeiro K, Carolina Barros de Castro M, Pratta Campos L, Paula Rosa Dos Santos A, Marino de Souza I, de Assis Bianchini M, Helena Ramiro Correa S, Ordones Baptista Luz R, Dos Santos Vieira A, Maria de Oliveira Pinto L, Azeredo E, Tadeu Moraes Figueiredo L, Augusto Fonseca Alencar J, Maria Barbosa de Lima S, Miraglia Herrera H, Dezengrini Shlessarenko R, Barreto Dos Santos F, Maria Bispo de Filippis A, Salyer S, Montgomery J, and Komar N
- Subjects
- Animals, Brazil epidemiology, Culicidae, Geography, Medical, Humans, Mosquito Vectors, Neutralization Tests, Public Health Surveillance, Seroepidemiologic Studies, Zika Virus Infection transmission, Zoonoses, Zika Virus, Zika Virus Infection epidemiology, Zika Virus Infection virology
- Abstract
Zika virus (ZIKV) was first discovered in 1947 in Uganda but was not considered a public health threat until 2007 when it found to be the source of epidemic activity in Asia. Epidemic activity spread to Brazil in 2014 and continued to spread throughout the tropical and subtropical regions of the Americas. Despite ZIKV being zoonotic in origin, information about transmission, or even exposure of non-human vertebrates and mosquitoes to ZIKV in the Americas, is lacking. Accordingly, from February 2017 to March 2018, we sought evidence of sylvatic ZIKV transmission by sampling whole blood from approximately 2000 domestic and wild vertebrates of over 100 species in West-Central Brazil within the active human ZIKV transmission area. In addition, we collected over 24,300 mosquitoes of at least 17 genera and 62 species. We screened whole blood samples and mosquito pools for ZIKV RNA using pan-flavivirus primers in a real-time reverse-transcription polymerase chain reaction (RT-PCR) in a SYBR Green platform. Positives were confirmed using ZIKV-specific envelope gene real-time RT-PCR and nucleotide sequencing. Of the 2068 vertebrates tested, none were ZIKV positive. Of the 23,315 non-engorged mosquitoes consolidated into 1503 pools tested, 22 (1.5%) with full data available showed some degree of homology to insect-specific flaviviruses. To identify previous exposure to ZIKV, 1498 plasma samples representing 62 species of domestic and sylvatic vertebrates were tested for ZIKV-neutralizing antibodies by plaque reduction neutralization test (PRNT
90 ). From these, 23 (1.5%) of seven species were seropositive for ZIKV and negative for dengue virus serotype 2, yellow fever virus, and West Nile virus, suggesting potential monotypic reaction for ZIKV. Results presented here suggest no active transmission of ZIKV in non-human vertebrate populations or in alternative vector candidates, but suggest that vertebrates around human populations have indeed been exposed to ZIKV in West-Central Brazil.- Published
- 2019
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43. Duration of post-vaccination humoral immunity against yellow fever in children.
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de Noronha TG, de Lourdes de Sousa Maia M, Geraldo Leite Ribeiro J, Campos Lemos JA, Maria Barbosa de Lima S, Martins-Filho OA, Campi-Azevedo AC, da Silva Freire M, de Menezes Martins R, and Bastos Camacho LA
- Subjects
- Antibodies, Neutralizing immunology, Antibodies, Viral immunology, Child, Female, Humans, Immunogenicity, Vaccine, Male, Time Factors, Vaccination legislation & jurisprudence, Vaccination methods, Vaccine Potency, Immunity, Humoral, Yellow Fever immunology, Yellow Fever prevention & control, Yellow Fever Vaccine immunology
- Abstract
Introduction: Vaccination is the most important measure for prevention and control of yellow fever. It is recommended by the World Health Organization (WHO) for residents of endemic areas and travelers to risk areas. In 2013, the WHO discontinued the recommendation of booster doses every 10 years, indicating a single dose as sufficient for lifelong protection., Objective: Considering the lower immune response to YF vaccine in children compared to adults, this study was set out to assess the duration of immunity to YF in children vaccinated in the first two years of life., Methods: This cross-sectional study involved children aged 9 months to 12 years with accessible vaccination records recruited in primary care units from a metropolitan area in Southeast Brazil. The serologic status (negative, indeterminate and positive), and geometric mean titers (GMT, inverse dilution) of neutralizing antibodies against YF obtained by Plaque Reduction Neutralization Test was assessed across categories of time after YF vaccination. The strength of association of seropositivity with time was assessed by the odds ratio (OR) taking recent vaccination (1-6 months) as reference., Results: A total of 824 children recruited from August 2010 to July 2011were tested. The proportion of seropositivity (95% C.I.) and GMT (95% C.I.) dropped markedly across time periods: from 86.7% (80.5-91.4%), GMT 47.9 (38.3-59.9) in newly vaccinated to 59.0% (49.7-67.8%), GMT 14.8 (11.6-19.1) and 42.2% (33.8-51.0), GMT 8.6 (7.1-12.1), respectively in the subgroups vaccinated 31-72 months and 73-100 months before., Conclusions: Analogous to previous findings in adults, these data support the need for revaccination of children living in areas with yellow fever virus circulation in humans or in other primates. The data also supported the change of a booster dose to 4 years of age for those primarily vaccinated for yellow fever in the first two years of life., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2019
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44. Optic nerve regeneration: A long view.
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Yin Y, De Lima S, Gilbert HY, Hanovice NJ, Peterson SL, Sand RM, Sergeeva EG, Wong KA, Xie L, and Benowitz LI
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- Animals, Axons metabolism, Axons ultrastructure, Humans, Inflammation Mediators metabolism, Optic Nerve ultrastructure, Optic Nerve Injuries pathology, Retinal Ganglion Cells ultrastructure, Nerve Regeneration physiology, Optic Nerve physiology, Optic Nerve Injuries metabolism, Retinal Ganglion Cells metabolism
- Abstract
The optic nerve conveys information about the outside world from the retina to multiple subcortical relay centers. Until recently, the optic nerve was widely believed to be incapable of re-growing if injured, with dire consequences for victims of traumatic, ischemic, or neurodegenerative diseases of this pathway. Over the past 10-20 years, research from our lab and others has made considerable progress in defining factors that normally suppress axon regeneration and the ability of retinal ganglion cells, the projection neurons of the retina, to survive after nerve injury. Here we describe research from our lab on the role of inflammation-derived growth factors, suppression of inter-cellular signals among diverse retinal cell types, and combinatorial therapies, along with related studies from other labs, that enable animals with optic nerve injury to regenerate damaged retinal axons back to the brain. These studies raise the possibility that vision might one day be restored to people with optic nerve damage.
- Published
- 2019
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45. Injection of bone marrow mesenchymal stem cells by intravenous or intraperitoneal routes is a viable alternative to spinal cord injury treatment in mice.
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Ramalho BDS, Almeida FM, Sales CM, de Lima S, and Martinez AMB
- Abstract
In spite of advances in surgical care and rehabilitation, the consequences of spinal cord injury (SCI) are still challenging. Several experimental therapeutic strategies have been studied in the SCI field, and recent advances have led to the development of therapies that may act on the inhibitory microenvironment. Assorted lineages of stem cells are considered a good treatment for SCI. This study investigated the effect of systemic transplantation of mesenchymal stem cells (MSCs) in a compressive SCI model. Here we present results of the intraperitoneal route, which has not been used previously for MSC administration after compressive SCI. We used adult female C57BL/6 mice that underwent laminectomy at the T
9 level, followed by spinal cord compression for 1 minute with a 30-g vascular clip. The animals were divided into five groups: sham (anesthesia and laminectomy but without compression injury induction), MSC i.p. (intraperitoneal injection of 8 × 105 MSCs in 500 µL of DMEM at 7 days after SCI), MSC i.v. (intravenous injection of 8 × 105 MSCs in 500 µL of DMEM at 7 days after SCI), DMEM i.p. (intraperitoneal injection of 500 µL of DMEM at 7 days after SCI), DMEM i.v. (intravenous injection of 500 µL of DMEM at 7 days after SCI). The effects of MSCs transplantation in white matter sparing were analyzed by luxol fast blue staining. The number of preserved fibers was counted in semithin sections stained with toluidine blue and the presence of trophic factors was analyzed by immunohistochemistry. In addition, we analyzed the locomotor performance with Basso Mouse Scale and Global Mobility Test. Our results showed white matter preservation and a larger number of preserved fibers in the MSC groups than in the DMEM groups. Furthermore, the MSC groups had higher levels of trophic factors (brain-derived neurotrophic factor, nerve growth factor, neurotrophin-3 and neurotrophin-4) in the spinal cord and improved locomotor performance. Our results indicate that injection of MSCs by either intraperitoneal or intravenous routes results in beneficial outcomes and can be elected as a choice for SCI treatment., Competing Interests: The authors declare no conflicts of interest- Published
- 2018
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46. In vitro and in vivo effects of ophthalmic solutions on silicone hydrogel bandage lens material Senofilcon A.
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Erdal NB, Adolfsson KH, De Lima S, and Hakkarainen M
- Subjects
- Humans, Microscopy, Electron, Scanning methods, Pilot Projects, Reproducibility of Results, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Bandages, Hydrocolloid, Contact Lenses, Hydrophilic, Hydrogels chemistry, Materials Testing methods, Ophthalmic Solutions pharmacology, Silicones chemistry
- Abstract
Background: Acuvue Oasys silicone hydrogel contact lenses (Senofilcon A) are used as bandage lenses and often combined with ophthalmic solutions in the treatment of ocular diseases. Concerns have been raised regarding the compatibility and effect of eye-drop solutions on the bandage lenses, which have led to frequent replacement of lenses causing clinical problems. Some patients experience pain or discomfort during treatments and the accumulation of drugs and preservatives in lenses has been suggested as a possible reason. The aim with this study was to investigate the effect of ophthalmic solutions on silicone hydrogel bandage lens material Senofilcon A in vitro and in vivo., Methods: The effect of three common ophthalmic solutions Isopto-Maxidex, Timosan and Oftaquix on Acuvue Oasys (Senofilcon A) bandage lenses was evaluated. An in vitro model method was developed where drug and preservative uptake by Acuvue Oasys was monitored with ultraviolet-visible spectroscopy and laser desorption ionisation mass spectrometry. Surface morphology changes of the lenses were evaluated using scanning electron microscopy. The method was then implemented for the in vivo pilot study evaluating lenses worn by patients., Results: In vitro model study monitoring the drug and preservatives uptake showed that the active ingredients from all the eye drops together with preservatives were taken up by the lenses in significant amounts. For the in vivo study no traces of active ingredients or preservatives could be found on the worn and treated lenses regardless of time being worn or dosage profiles. The surface morphology changes in the in vivo study were also minor in contrast to the changes observed in the in vitro scanning electron microscopy images., Conclusion: The in vivo results demonstrate minor effects of the ophthalmic solutions on the worn lenses. These results do not support the building up of preservatives and drugs on the contact lenses as the cause of pain or discomfort experienced by some patients, which is encouraging for the use of bandage lenses in combination with ophthalmic solutions., (© 2018 Optometry Australia.)
- Published
- 2018
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47. Study of the tumor microenvironment during breast cancer progression.
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Eftekhari R, Esmaeili R, Mirzaei R, Bidad K, de Lima S, Ajami M, Shirzad H, Hadjati J, and Majidzadeh-A K
- Abstract
Background: Different cells and mediators in the tumor microenvironment play important roles in the progression of breast cancer. The aim of this study was to determine the composition of the microenvironment during tumor progression in order to discover new related biomarkers and potentials for targeted therapy., Methods: In this study, breast cancer biopsies from four different stages, and control breast biopsies were collected. Then, the mRNA expression of several markers related to different CD4
+ T cell subsets including regulatory T cells (Treg), T helper (Th) type 1, 2 and 17 were determined. In addition, we investigated the expression of two inflammatory cytokines (TNF-α and IL-6) and inflammatory mediators including FASL, IDO, SOCS1, VEGF, and CCR7., Results: The results showed that the expression of Th1 and Th17 genes was decreased in tumor tissues compared to control tissues. In addition, we found that the gene expression related to these two cell subsets decreased during cancer progression. Moreover, the expression level of TNF-α increased with tumor progression., Conclusion: We conclude that the expression of genes related to immune response and inflammation is different between tumor tissues and control tissues. In addition, this difference was perpetuated through the different stages of cancer.- Published
- 2017
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48. N-myc regulates growth and fiber cell differentiation in lens development.
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Cavalheiro GR, Matos-Rodrigues GE, Zhao Y, Gomes AL, Anand D, Predes D, de Lima S, Abreu JG, Zheng D, Lachke SA, Cvekl A, and Martins RAP
- Subjects
- Animals, Cell Nucleus metabolism, Cell Proliferation genetics, Cell Survival genetics, Embryonic Development genetics, Gene Expression Regulation, Developmental, Lens, Crystalline metabolism, Mice, Proto-Oncogene Proteins c-myc metabolism, Transcription, Genetic, Transcriptome genetics, Cell Differentiation genetics, Lens, Crystalline cytology, Lens, Crystalline growth & development, N-Myc Proto-Oncogene Protein metabolism
- Abstract
Myc proto-oncogenes regulate diverse cellular processes during development, but their roles during morphogenesis of specific tissues are not fully understood. We found that c-myc regulates cell proliferation in mouse lens development and previous genome-wide studies suggested functional roles for N-myc in developing lens. Here, we examined the role of N-myc in mouse lens development. Genetic inactivation of N-myc in the surface ectoderm or lens vesicle impaired eye and lens growth, while "late" inactivation in lens fibers had no effect. Unexpectedly, defective growth of N-myc-deficient lenses was not associated with alterations in lens progenitor cell proliferation or survival. Notably, N-myc-deficient lens exhibited a delay in degradation of DNA in terminally differentiating lens fiber cells. RNA-sequencing analysis of N-myc-deficient lenses identified a cohort of down-regulated genes associated with fiber cell differentiation that included DNaseIIβ. Further, an integrated analysis of differentially expressed genes in N-myc-deficient lens using normal lens expression patterns of iSyTE, N-myc-binding motif analysis and molecular interaction data from the String database led to the derivation of an N-myc-based gene regulatory network in the lens. Finally, analysis of N-myc and c-myc double-deficient lens demonstrated that these Myc genes cooperate to drive lens growth prior to lens vesicle stage. Together, these findings provide evidence for exclusive and cooperative functions of Myc transcription factors in mouse lens development and identify novel mechanisms by which N-myc regulates cell differentiation during eye morphogenesis., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2017
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49. Shared decision making in Brazil: Concrete efforts to empower the patients' voice.
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Mendes de Abreu M, Simao de Mello JP, Ferreira F Ribeiro L, Andrade Mussi L, L Borges ML, Petroli M, da Costa Tavares N, da Cunha Cancela R, and Fausto de Lima S
- Subjects
- Brazil, Health Policy, Humans, Personal Autonomy, Physician-Patient Relations, Decision Making, Patient Participation
- Abstract
Patient involvement in healthcare decisions has grown in Brazil at three different levels: 1) the macro level, which includes the patient actively influencing legislation and regulation of medical care as well as political changes in the process of care itself; 2) the meso level, which includes institutions that aim to improve information, empowerment and counseling to patients, and 3) the micro level, which focuses on the actual decision-making process that takes place within patient-physician encounter. In Brazil, the macro and meso levels are stronger than the micro one. In this paper, the practical efforts to engage patients in the center of their own care are presented. In order to do that, an overview on the National Humanization Policy and the Brazilian patient's movement is provided., (Copyright © 2017. Published by Elsevier GmbH.)
- Published
- 2017
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50. Mobile zinc increases rapidly in the retina after optic nerve injury and regulates ganglion cell survival and optic nerve regeneration.
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Li Y, Andereggen L, Yuki K, Omura K, Yin Y, Gilbert HY, Erdogan B, Asdourian MS, Shrock C, de Lima S, Apfel UP, Zhuo Y, Hershfinkel M, Lippard SJ, Rosenberg PA, and Benowitz L
- Subjects
- Animals, Carrier Proteins genetics, Carrier Proteins physiology, Cation Transport Proteins, Chelating Agents pharmacology, Ethylamines pharmacology, Male, Membrane Proteins genetics, Membrane Proteins physiology, Membrane Transport Proteins, Mice, Inbred C57BL, Mice, Knockout, Pyridines pharmacology, Sulfanilic Acids pharmacology, Nerve Regeneration, Optic Nerve physiology, Optic Nerve Injuries metabolism, Retina physiology, Zinc metabolism
- Abstract
Retinal ganglion cells (RGCs), the projection neurons of the eye, cannot regenerate their axons once the optic nerve has been injured and soon begin to die. Whereas RGC death and regenerative failure are widely viewed as being cell-autonomous or influenced by various types of glia, we report here that the dysregulation of mobile zinc (Zn
2+ ) in retinal interneurons is a primary factor. Within an hour after the optic nerve is injured, Zn2+ increases several-fold in retinal amacrine cell processes and continues to rise over the first day, then transfers slowly to RGCs via vesicular release. Zn2+ accumulation in amacrine cell processes involves the Zn2+ transporter protein ZnT-3, and deletion of slc30a3, the gene encoding ZnT-3, promotes RGC survival and axon regeneration. Intravitreal injection of Zn2+ chelators enables many RGCs to survive for months after nerve injury and regenerate axons, and enhances the prosurvival and regenerative effects of deleting the gene for phosphatase and tensin homolog (pten). Importantly, the therapeutic window for Zn2+ chelation extends for several days after nerve injury. These results show that retinal Zn2+ dysregulation is a major factor limiting the survival and regenerative capacity of injured RGCs, and point to Zn2+ chelation as a strategy to promote long-term RGC protection and enhance axon regeneration., Competing Interests: The authors declare no conflict of interest.- Published
- 2017
- Full Text
- View/download PDF
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