1. M. tuberculosis infection and antigen specific cytokine response in healthcare workers frequently exposed to tuberculosis
- Author
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Howard Takiff, Brigitte Gicquel, Marielle Leboueny, Patrice Hemery Dapnet Tadatsin, Anicet Christel Maloupazoa Siawaya, Oriane Cordelia Aboumegone Biyogo, Ofilia Mvoundza Ndjindji, Dimitri Ulrich Essamazokou, Amel Kévin Alame-Emane, Guy-Stéphane Padzys, Amandine Mveang Nzoghe, Selidji T Agnandji, Joel Fleury Djoba Siawaya, and Paulin N. Essone
- Subjects
0301 basic medicine ,Adult ,Male ,Tuberculosis ,medicine.medical_treatment ,Health Personnel ,lcsh:Medicine ,Article ,Proinflammatory cytokine ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Antigen ,Immunity ,Latent Tuberculosis ,Occupational Exposure ,medicine ,Odds Ratio ,Humans ,Antigens ,lcsh:Science ,Whole blood ,Inflammation ,Antigens, Bacterial ,Multidisciplinary ,Anthropometry ,business.industry ,lcsh:R ,Mycobacterium tuberculosis ,Middle Aged ,medicine.disease ,Publisher Correction ,Immunity, Innate ,Occupational Diseases ,030104 developmental biology ,Cytokine ,Cross-Sectional Studies ,Immunology ,Cytokines ,Tumor necrosis factor alpha ,Female ,lcsh:Q ,business ,030217 neurology & neurosurgery ,Biomarkers - Abstract
Tuberculosis (TB) is the leading cause of death due to an infectious agent, but only a small fraction of those infected develop the disease. Cytokines are involved in the mediation and regulation of immunity, and their secretion patterns may reflect the infection status. To increase our understanding of immune response to M. tuberculosis infection, we conducted a cross-sectional study investigating M. tuberculosis infection status and comparing the release profiles of cytokines GM-CSF, IFN-γ, IL-1β, IL-10, IL-12 (p70), IL-2, IL-4, IL-5, IL-6, IL-8, TNF-α, in community controls (CCs) and healthy healthcare workers (HCWs) highly exposed to TB. Among HCWs and CCs, the probability of latent M. tuberculosis (LTB+) infection was respectively 5.4 (p = 0.002) and 3.4 (p = 0.006) times higher in men than women. The odds ratio of LTB infection was 4 times higher among HCWs in direct contact with active TB patients than other HCW (p = 0.01). Whole blood supernatant cytokine responses to M. tuberculosis antigens showed differential pro-inflammatory responses between HCWs and CCs. CCsLTB− had higher IL-1β responses than HCWsLTB− (p = 0.002). HCWsLTB+ had significantly higher IL-8 responses to M. tuberculosis antigens than HCWsLTB− (p = 0.003) and CCsLTB− (p = 0.015). HCWsLTB+/− showed weak but positive TNF-α responses to M. tuberculosis antigen stimulation compared to CCsLTB+/− (p ≤ 0.015). Looking at T-helper (1 and 2) responses, HCWsLTB+ and CCsLTB+ had significantly higher IFN-γ and IL-2 responses compared to HCWsLTB− and CCsLTB− (p LTB+ and CCsLTB+ than in non-infected CCsLTB− (p M. tuberculosis antigen specific responses in HCWsLTB+ varied based on active TB exposure gradient. HCWsLTB+ who were highly exposed to active TB (≥3 hours per day) had significantly higher IFN-γ and IL-8 responses (p ≤ 0.02) than HCWs LTB+ not in direct contact with active TB patients. HCWsLTB+ working with active TB patients for 5 to 31 years had a significantly enhanced secretion of proinflammatory cytokines (GM-CSF, IFN-γ, IL-1β, IL-2, IL-6, IL-8, IL-12p70, TNF-α) compared to HCWsLTB− (p LTB+ than HCWsLTB−. In conclusion, LTBI individuals controlling the M. tuberculosis infection have an enhanced TB specific Th1-cytokines/proinflammatory response combined with selected Th2 type/anti-inflammatory cytokines induction.
- Published
- 2019