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3. MAP6 interacts with Tctex1 and Cav 2.2/N-type calcium channels to regulate calcium signalling in neurons

Author

Brocard, Jacques, Dufour, Fabrice, Gory‐Fauré, Sylvie, Arnoult, Christophe, Bosc, Christophe, Denarier, Eric, Peris, Leticia, Saoudi, Yasmina, De Waard, Michel, Andrieux, Annie, Physiopathologie du Cytosquelette, [GIN] Grenoble Institut des Neurosciences (GIN), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), INSERM U836, équipe 1, Physiopathologie du cytosquelette, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Inserm, U1216, Grenoble, France., Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), Groupe Physiopathologie du Cytosquelette (GPC), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), unité de recherche de l'institut du thorax UMR1087 UMR6291 (ITX), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Unité de recherche de l'institut du thorax (ITX-lab), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), and Université de Nantes (UN)-Université de Nantes (UN)

Subjects
Mice, Knockout, Neurons, vesicles, Binding Sites, yeast two-hybrid, hippocampal neurons, [SDV]Life Sciences [q-bio], Dyneins, in vitro interactions, Molecular and Synaptic Mechanisms, plasma membrane, Hippocampus, microtubules, Mice, Calcium Channels, N-Type, Animals, Female, Calcium Signaling, yeast two‐hybrid, Microtubule-Associated Proteins, Cells, Cultured, Protein Binding
Abstract

International audience; MAP6 proteins were first described as microtubule-stabilizing agents, whose properties were thought to be essential for neuronal development and maintenance of complex neuronal networks. However, deletion of all MAP6 isoforms in MAP6 KO mice does not lead to dramatic morphological aberrations of the brain but rather to alterations in multiple neurotransmissions and severe behavioural impairments. A search for protein partners of MAP6 proteins identified Tctex1 - a dynein light chain with multiple non-microtubule-related functions. The involvement of Tctex1 in calcium signalling led to investigate it in MAP6 KO neurons. In this study, we show that functional Cav 2.2/N-type calcium channels are deficient in MAP6 KO neurons, due to improper location. We also show that MAP6 proteins interact directly with both Tctex1 and the C-terminus of Cav 2.2/N-type calcium channels. A balance of these two interactions seems to be crucial for MAP6 to modulate calcium signalling in neurons.

Published
2017
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4. Continuous Downstream Processing of Amino Acids in a Modular Miniplant.

Author

Hohmann, Lukas, Löbnitz, Lisa, Menke, Christina, Santhirakumaran, Bavatharani, Stier, Patrick, Stenger, Frank, Dufour, Fabrice, Wiese, Georg, zur Horst-Meyer, Santer zur, Kusserow, Burkhard, Zang, Werner, Nirschl, Hermann, and Kockmann, Norbert

Subjects
AMINO acids, CHEMICAL industry, CRYSTALLIZATION, BATCH processing, MODULAR design, EVAPORATORS
Abstract

Abstract: Global competition leads to a need for a fast time to market and increased resource efficiency. Continuous processing, module‐based plant design, and multipurpose equipment are recently discussed approaches for the fine‐chemical industry. As a representative, the downstream process of amino acid production is discussed herein. A conventional batch procedure was transferred to a continuous process and realized in a modular miniplant, which comprised of evaporation in a wiped‐film evaporator, seeded cooling crystallization in a coiled tubular crystallizer, and solid/liquid separation on a vacuum belt filter. The operations were realized on‐skid with individual automation systems and integrated sensors. L‐Alanine was successfully processed in steady‐state operation. [ABSTRACT FROM AUTHOR]

Published
2018
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5. MAP6 interacts with Tctex1 and Cav2.2/N-type calcium channels to regulate calcium signalling in neurons.

Author

Brocard, Jacques, Dufour, Fabrice, Gory‐Fauré, Sylvie, Arnoult, Christophe, Bosc, Christophe, Denarier, Eric, Peris, Leticia, Saoudi, Yasmina, De Waard, Michel, and Andrieux, Annie

Subjects
*HIPPOCAMPUS (Brain), *NEURONS, *MICROTUBULES, *CELL membranes, *SYNAPTIC vesicles
Abstract

MAP6 proteins were first described as microtubule-stabilizing agents, whose properties were thought to be essential for neuronal development and maintenance of complex neuronal networks. However, deletion of all MAP6 isoforms in MAP6 KO mice does not lead to dramatic morphological aberrations of the brain but rather to alterations in multiple neurotransmissions and severe behavioural impairments. A search for protein partners of MAP6 proteins identified Tctex1 - a dynein light chain with multiple non-microtubule-related functions. The involvement of Tctex1 in calcium signalling led to investigate it in MAP6 KO neurons. In this study, we show that functional Cav2.2/N-type calcium channels are deficient in MAP6 KO neurons, due to improper location. We also show that MAP6 proteins interact directly with both Tctex1 and the C-terminus of Cav2.2/N-type calcium channels. A balance of these two interactions seems to be crucial for MAP6 to modulate calcium signalling in neurons. [ABSTRACT FROM AUTHOR]

Published
2017
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6. MAP6 interacts with Tctex1 and Ca v 2.2/N-type calcium channels to regulate calcium signalling in neurons.

Author

Brocard J, Dufour F, Gory-Fauré S, Arnoult C, Bosc C, Denarier E, Peris L, Saoudi Y, De Waard M, and Andrieux A

Subjects
Animals, Binding Sites, Cells, Cultured, Female, Hippocampus cytology, Mice, Mice, Knockout, Microtubule-Associated Proteins genetics, Protein Binding, Calcium Channels, N-Type metabolism, Calcium Signaling physiology, Dyneins metabolism, Microtubule-Associated Proteins metabolism, Neurons metabolism
Abstract

MAP6 proteins were first described as microtubule-stabilizing agents, whose properties were thought to be essential for neuronal development and maintenance of complex neuronal networks. However, deletion of all MAP6 isoforms in MAP6 KO mice does not lead to dramatic morphological aberrations of the brain but rather to alterations in multiple neurotransmissions and severe behavioural impairments. A search for protein partners of MAP6 proteins identified Tctex1 - a dynein light chain with multiple non-microtubule-related functions. The involvement of Tctex1 in calcium signalling led to investigate it in MAP6 KO neurons. In this study, we show that functional Ca v 2.2/N-type calcium channels are deficient in MAP6 KO neurons, due to improper location. We also show that MAP6 proteins interact directly with both Tctex1 and the C-terminus of Ca v 2.2/N-type calcium channels. A balance of these two interactions seems to be crucial for MAP6 to modulate calcium signalling in neurons., (© 2017 The Authors. European Journal of Neuroscience published by Federation of European Neuroscience Societies and John Wiley & Sons Ltd.)

Published
2017
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