1. Sodium Selenite Enhanced the Anti-proliferative Effect of MEK-ERK Inhibitor in Thyroid Cancer Cells
- Author
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Woosung Lim, Byung In Moon, Hyungju Kwon, Jong Bin Kim, Eun Yeol Yang, and Joohyun Woo
- Subjects
MAPK/ERK pathway ,Cancer Research ,endocrine system diseases ,MAP Kinase Signaling System ,Erk signaling ,MAP Kinase Kinase 1 ,chemistry.chemical_element ,Apoptosis ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Sodium Selenite ,Western blot ,Nitriles ,medicine ,Butadienes ,Tumor Cells, Cultured ,Humans ,Viability assay ,Thyroid Neoplasms ,Enzyme Inhibitors ,Thyroid cancer ,Cell Proliferation ,Pharmacology ,Mitogen-Activated Protein Kinase 1 ,Mitogen-Activated Protein Kinase 3 ,medicine.diagnostic_test ,Chemistry ,Drug Synergism ,Anti proliferative ,medicine.disease ,Trace Elements ,030220 oncology & carcinogenesis ,Toxicity ,Cancer research ,Drug Therapy, Combination ,sense organs ,Selenium ,Research Article - Abstract
Background/Aim: MEK-ERK pathway plays major roles in the progression of thyroid cancer, while the use of MEK-ERK inhibitors has been limited by its toxicity. We investigated the effect of sodium selenite as an adjunct for MEK-ERK inhibitors to avoid the toxicity of ERK inhibitors. Materials and Methods: TPC1, 8505C and HTori-3 cells were treated with U0126 (MEK-ERK inhibitor) and cell viability was counted in the Neubauer chamber. The synergistic effects of sodium selenite and U0126 were also measured. The expression of ERK, p-ERK, and p90(RSK) was determined by western blot. Results: Treatment with U0126 inhibited proliferation of TPC1 and 8505C cells in a dose-dependent manner. When 5 μM sodium selenite was added to 1 μM U0126, relative cell survival further decreased. Decreased expression of p90(RSK )indicated that sodium selenite down-regulated ERK signaling in thyroid cancer cells. Conclusion: The combination of U0126 and sodium selenite inhibited proliferation of thyroid cancer cells through ERK inhibition.
- Published
- 2020