1. A metagenome-wide association study of gut microbiome and visceral fat accumulation
- Author
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Xiaojing Ma, Haoyong Yu, Yuqian Bao, Xiaomin Nie, Gianni Panagiotou, Yun Shen, Jiarui Chen, and Yueqiong Ni
- Subjects
VFA, visceral fat area ,GPR43, G-protein coupled receptor 43 ,BMI, body mass index ,Cr, creatinine ,Physiology ,AST, aspartate aminotransferase ,Disease ,SCFAs, short chain fatty acids ,Biochemistry ,0302 clinical medicine ,WBC, white blood cell count ,WHR, waist-to-hip ratio ,Structural Biology ,Medicine ,Visceral fat ,0303 health sciences ,TG, triglyceride ,FMT, fecal microbiota transplantation ,SFA, subcutaneous fat area ,LSG, laparoscopic sleeve gastrectomy ,UA, uric acid ,Computer Science Applications ,030220 oncology & carcinogenesis ,TCA, tricarboxylic acid cycle ,Research Article ,Biotechnology ,MSG, monosodium glutamate ,Waist ,FDR, false discovery rate ,HDL, high-density lipoprotein cholesterol ,lcsh:Biotechnology ,2hCP, 2-hour C-peptide ,Biophysics ,DBP, diastolic blood pressure ,03 medical and health sciences ,2hPG, 2-hour plasma glucose ,ALT, alanine aminotransferase ,lcsh:TP248.13-248.65 ,Genetics ,Carbohydrate fermentation ,FPG, fasting plasma glucose ,Obesity ,Risk factor ,BCAAs, branched chain amino acids ,ComputingMethodologies_COMPUTERGRAPHICS ,030304 developmental biology ,Gut microbiome ,business.industry ,SBP, systolic blood pressure ,CoDA, Compositional Data Analysis ,LDL, low-density lipoprotein cholesterol ,medicine.disease ,TC, total cholesterol ,Laparoscopic sleeve gastrectomy ,FCp, fasting C-peptide ,Metagenomics ,LPS, lipopolysaccharides ,business ,MRI, magnetic resonance imaging ,Body mass index ,HbA1c, glycated hemoglobin A1c - Abstract
Graphical abstract, Purpose Visceral fat is an independent risk factor for metabolic and cardiovascular disease. The study aimed to investigate the associations between gut microbiome and visceral fat. Methods We recruited 32 obese adults and 30 healthy controls at baseline. Among the obese subjects, 14 subjects underwent laparoscopic sleeve gastrectomy (LSG) and were followed 6 months after surgery. Abdominal visceral fat area (VFA) and subcutaneous fat area (SFA) were measured by magnetic resonance imaging. Waist, hipline, waist-to-hip ratio (WHR) and body mass index (BMI) were included as simple obese parameters. Gut microbiome was analyzed by metagenomic sequencing. Results Among the obese parameters, VFA had the largest number of correlations with the species that were differentially enriched between obese and healthy subjects, following by waist, WHR, BMI, hipline, and SFA. Within the species negatively correlated with VFA, Eubacterium eligens had the strongest correlation, following by Clostridium citroniae, C. symbiosum, Bacteroides uniformis, E. ventriosum, Ruminococcaceae bacterium D16, C. hathewayi, etc. C. hathewayi and C. citroniae were increased after LSG. Functional analyses showed that among all the obese parameters, VFA had strongest correlation coefficients with the obesity-related microbial pathways. Microbial pathways involved in carbohydrate fermentation and biosynthesis of L-glutamate and L-glutamine might contribute to visceral fat accumulation. Conclusions Visceral fat was more closely correlated with gut microbiome compared with subcutaneous fat, suggesting an intrinsic connection between gut microbiome and metabolic cardiovascular diseases. Specific microbial species and pathways which were closely associated with visceral fat accumulation might contribute to new targeted therapies for metabolic disorders.
- Published
- 2020