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Selective separation and enrichment of phosphoproteins are essential for understanding their important functions in almost all cellular processes. Here, taking advantage of the feature that cadmium ion (Cd2+) has an overwhelming preference for phosphates, we developed a robust and simple Cd2+ co‐precipitation strategy for the selective isolation of intact phosphoproteins. After evaluating the feasibility of Cd2+ in phosphoprotein precipitation, we compared the washing protocols for the removal of non‐specific binding proteins and then used the best‐performing protocol for the isolation of phosphoproteins from different complex samples. It was found that phosphoproteins can be specifically enriched from artificial protein mixtures containing α‐casein, β‐casein, and bovine serum albumin or plasma, in which bovine serum albumin or plasma were served as interferences with very high molar ratios. Applying this method to enrich phosphoproteins from complex cell lysates, a high specificity was confirmed by western blotting analysis with a phosphoprotein‐specific kit. Finally, we successfully applied this method to the purification of caseins from drinking milk, highlighting its potential application in the studies where purified phosphoproteins were required. In a word, this Cd2+ co‐precipitation method enables universal and effective capture, enrichment, and detection of intact phosphoproteins, making it a powerful tool for the comprehensive analysis of the phosphoproteome. [ABSTRACT FROM AUTHOR]

Fully polarimetric synthetic aperture radar (PolSAR) data are represented by four channels and the coherences between two arbitrary channels are important descriptors for the interpretation of polarimetric scattering mechanisms. However, there exists a scattering ambiguity between oriented urban and natural areas since they both contribute to the cross‐polarised (HV) scattering. A polarimetric correlation coefficient ratio is proposed to overcome this scattering ambiguity in PolSAR imagery. First, the polarimetric coherences of urban areas with various orientation angles were analysed. Subsequently, it was found that the proposed coherence parameter considers the reflection asymmetry of buildings and also the scattering divergence between co‐polarised and cross‐polarised channels, which is beneficial to the scattering analysis in urban areas. The effectiveness of the proposed parameter is demonstrated using the Phased Array type L‐band Synthetic Aperture Radar dataset. [ABSTRACT FROM AUTHOR]