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74 results on '"Ferchaud, Stéphane"'

1. Cell specification and functional interactions in the pig blastocyst inferred from single-cell transcriptomics and uterine fluids proteomics

Author: Dufour, Adrien, Kurylo, Cyril, Stöckl, Jan B., Laloë, Denis, Bailly, Yoann, Manceau, Patrick, Martins, Frédéric, Turhan, Ali G., Ferchaud, Stéphane, Pain, Bertrand, Fröhlich, Thomas, Foissac, Sylvain, Artus, Jérôme, and Acloque, Hervé
Published: 2024
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2. Dental microwear textures differ in pigs with overall similar diets but fed with different seeds

Author: Louail, Margot, Ferchaud, Stéphane, Souron, Antoine, Walker, Axelle E.C., and Merceron, Gildas
Published: 2021
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3. Seminal plasma differentially alters the resistance of dog, ram and boar spermatozoa to hypotonic stress

Author: Tsikis, Guillaume, Reynaud, Karine, Ferchaud, Stéphane, and Druart, Xavier
Published: 2018
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4. Improving piglet survival under organic condition

Author: Canario, Laurianne, Larzul, Catherine, Ferchaud, Stéphane, Moreau, Stéphane, Merlot, Elodie, Clouard, Caroline, Tallet, Céline, and Prunier, Armelle
Subjects: Breeding and genetics, Health and welfare
Abstract: This presentation describes the methodology and the first results of an experiment of genetic selection for lower piglet mortality. Young females (G1 generation) were chosen from sows (G0) reared in conventional herds. G1 females were raised from weaning in the organic pig farm from INRAE (Porganic). G0 sows were selected for high piglet survival, large number of weaned piglets, and indications from breeders about their ease of farrowing and good maternal behaviour. G0 and G1 females were inseminated with semen from boars selected for high breeding values. G1 females were conducted in batches of 12 females, half being crated around farrowing and half being loose around farrowing. Performance of G1 females after their 1st, 2nd and 3rd farrowing events are presented. These preliminary results suggest that piglet mortality is similar in both systems when calculated on the overall lactation but mortality seems to be slightly delayed in crated compared to loose-housed sows. First results also suggest that the piglet mortality is influenced by the behaviour of sows, even more in the situation of temporary crating around farrowing. These preliminary data were obtained within the Era-Net CORE Organic Cofund project (Power project: https://projects.au.dk/coreorganiccofund/core-organic-cofund-projects/power/). The experiment is continuing within the H2020 PPILOW project (https://www.ppilow.eu/).
Published: 2022

5. The PPILOW project: Innovations improving welfare in low input and organic pig and poultry farms

Author: Collin, Anne, Meloni, Giuditta, Bonnefous, Claire, Re, Martina, van Vooren, Laura, Niemi, Jarkko, Väre, Minna, Lähtinen, Katja, Tuyttens, Frank A.M., Graat, Evelien, Vanden Hole, Charlotte, Rodenburg, Tb (bas), Kliphuis, Saskia, Giersberg, Mona, Tavares, Olivia, Desaint, Brieuc, Steenfeldt, Sanna, Pedersen, Lene, Engberg, Ricarda M., Almadani Mohamad, Isam, Carelli, Riccardo, Sciarretta, Marlene, Guilloteau, Laurence, Réhault-Godbert, Sophie, Gautron, Joël, Le Bihan-Duval, Elisabeth, Mignon-Grasteau, Sandrine, Berri, Cécile, Guettier, Elodie, Baéza, Elisabeth, Chartrin, Pascal, Bordeau, Thierry, Raynaud, Emilie, Couroussé, Nathalie, Cailleau-Audouin, Estelle, Crochet, Sabine, Tourneur, Léa, Guichaoua, Adrien, van den Brand, Henry, Castellini, Cesare, Reverchon, Maxime, Sourdioux, Michel, Akakpo, Roland, Rangel Pedersen, Ninfa, Schepens, Rene, Almind, Maria, Grenier, Katia, Dubuc, David, Le Lann, Marie-Véronique, Ponzio, Raffaella, Mainardi, Marina, Accotto, Caterina, Coletta, Monica, Guesdon, Vanessa, Leruste, Hélène, Billiard, Bérangère, Ferreira, Vitor Hugo Bessa, Baldinger, Lisa, Pluschke, Helen, Delanoue, Elsa, Laura, Warin, Pertusa, Marion, Stomp, Mathilde, Travel, Angelique, Bouvarel, Isabelle, Germain, Karine, Ravon, Laure, Calandreau, Ludovic, Labas, Valérie, Prunier, Armelle, Merlot, Elodie, Tallet, Céline, Perruchot, Marie-Hélène, Louveau, Isabelle, van Milgen, Jaap, Dessauge, Frederic, Clouard, Caroline, Lebret, Bénédicte, Montagne, Lucile, Faure, Justine, Zuliani, Anna, Venezia, Pietro, Canario, Laurianne, Ferchaud, Stéphane, Cozma, Vasile, Spinu, Marina, Horia Bǎieş, Mihai, Courboulay, Valérie, Roguet, Christine, Gaudré, Didier, Chevillon, Patrick, Alibert, Laurent, Decruyenaere, Virginie, Wavreille, José, Vanggaard, Pia, Vanggaard, Jan, Micheloni, Cristina, Thobe, Petra, Lombard, Sarah, Caillaud, Laura, Bernardet, Nelly, Collet, Julie, Molenaar, Roos, Mattioli, Simona, Hill, Nigel, Cadudal, François, Quentin, Maxime, Leterrier, Christine, Teixeira-Gomes, Ana-Paula, Uzbekova, Svetlana, Maugrion, Emilie, Rocchi, Lucia, Biologie des Oiseaux et Aviculture (BOA), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Università degli Studi di Perugia = University of Perugia (UNIPG), Associazione Italiana per l'Agricoltura Biologica (AIAB), Scuela Santa Anna (SSSA), Scuola Universitaria Superiore Sant'Anna [Pisa] (SSSUP), BioForum Vlaanderen, Natural Resources Institute Finland (LUKE), Research Institute for Agricultural, Fisheries and Food (ILVO), Utrecht University [Utrecht], Institut Technique de l'Agriculture Biologique (ITAB), Aarhus University [Aarhus], Thuenen Institute of Farm Economics, European Association for Animal Production (EAAP), Les instituts techniques agricoles (Acta), Wageningen University and Research [Wageningen] (WUR), Syndicat des Sélectionneurs Avicoles et Aquacoles Français (SYSAAF), Fermentation Experts, Équipe Micro et nanosystèmes HyperFréquences Fluidiques (LAAS-MH2F), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Équipe DIagnostic, Supervision et COnduite (LAAS-DISCO), Slow Food Biodiversity, JUNIA (JUNIA), Université catholique de Lille (UCL), Thuenen-Institute of Organic Farming, ITAVI, Elevage Alternatif et Santé des Monogastriques (UE EASM), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), SIVtro VSF Italia, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Expérimentale Elevages Porcins Innovants (GenESI), USAMV Cluj-Napoca, Institut du Porc (IFIP), Centre Wallon de Recherches Agronomiques (CRA-W), Vanggaard Staldmontage, Harper Adams University, EAAP, PPILOW, and European Project: 816172,H2020-EU.3.2.1.1. - Increasing production efficiency and coping with climate change, while ensuring sustainability and resilience ,PPILOW (2019)
Subjects: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SCCO]Cognitive science, [SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SHS]Humanities and Social Sciences
Abstract: International audience; The PPILOW project aims to co-construct innovations to improve Poultry and Pig Welfare in Low-input outdoor and Organic farming systems through a multi-actor approach. PPILOW implements a participatory approach for proposing and studying welfare-improvement levers. It will provide a combination of practical solutions that can be applied at a pan-European level with specific adjustments depending on citizen’s expectations and the target market. The multi-actor approach consists in involving end-users including farmers, breeding companies, feed producers, consumer associations, retailers, advisers, processors, and scientists in National Practitioner Groups (NPG) in six participating countries. PPILOW partners facilitate the groups by connecting NPG at European level, transferring scientific information, interacting with partners engaged in animal experiments, and co-creating innovations rising from NPG-specific demands. They co-build with PPILOW partners welfare self-assessment tools (development of the PIGLOW app for pigs and refinement of the EBENE® app for poultry), and innovative breeding, feeding, and rearing strategies and techniques to improve the welfare of animals. They co-design protocols, test innovations on farm, and disseminate the results. In turn, they receive insights on methods and scientific results, and inputs from other NPG reinforcing the value of the expected outcomes. Approaches focus on avoiding physical damage and the elimination of layer male chicks, on reducing boar taint of intact male pigs, promoting positive behaviours, animal health, and robustness through field studies with pigs and poultry. Multicriteria analyses of the most effective levers of welfare improvement will be performed to evaluate their economic, social, and environmental impacts based on the ‘One Welfare’ concept; economic and business models will also be developed. To ensure the rapid uptake of the project results by end-users, the close involvement of PPILOW’s NPG throughout the EU will ensure disseminationactivities and the facilitation of change. The PPILOW project has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement N°816172. www.ppilow.eu.
Published: 2022

6. Meiotic pairing and gene expression disturbance in germ cells from an infertile boar with a balanced reciprocal autosome-autosome translocation

Author: Barasc, Harmonie, Congras, Annabelle, Mary, Nicolas, Trouilh, Lidwine, Marquet, Valentine, Ferchaud, Stéphane, Raymond-Letron, Isabelle, Calgaro, Anne, Loustau-Dudez, Anne-Marie, Mouney-Bonnet, Nathalie, Acloque, Hervé, Ducos, Alain, and Pinton, Alain
Published: 2016
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7. Conceptions et pratiques de gestion de la santé des animaux en productions animales sous cahier des charges de l'agriculture biologiquebiologiques

Author: Bareille, Nathalie, Duval, Julie, Experton, Catherine, Ferchaud, Stéphane, Hellec, Florence, Manoli, Claire, Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR), École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Territoires (Territoires), AgroParisTech-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Institut Technique de l'Agriculture Biologique (ITAB), Unité Expérimentale Elevages Porcins Innovants (GenESI), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme de géochimie isotopique ASTER-CEREGE, Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Recherche sur les Systèmes d'Elevage (URSE), and Ecole supérieure d'Agricultures d'Angers (ESA)
Subjects: [SDV]Life Sciences [q-bio]
Abstract: International audience; In order to contribute to the development of Organic Agriculture (OA) in France, this article provides elements for the understanding of its regulatory framework and particularities. First of all, the analysis of sociotechnical literature shows that organic farmers have a specific view of animal health which is based on a preventive and holistic approach to animal health, according to which health management is primarily based on levers related to the management of the farming system. Farmers surround themselves with a diversity of actors to accompany them in the management of health at the farm level, which goes beyond the classical advice of the veterinarian. Then, another particularity is that the health status of the herds seems to be better in OA than in conventional agriculture. Indeed, comparative studies show a lower frequency of treatment of clinically expressed diseases in AB, without it being clear whether this is due to a lower level of medication used by farmers. Finally, a focus is made on certain rearing practices, imposed or recommended by the specifications for reasons of health or biodiversity preservation. These practices have been widely adopted by organic farmers and have had positive impacts on animal health. These particularities have been addressed in the literature unequally depending on the animal production sector. Therefore, in this review, more detailed elements are given on ruminants, and some specific contributions are made concerning pig and poultry productions.; Afin de contribuer au développement continu de l’Agriculture Biologique (AB) en France, cet article apporte des éléments de compréhension de son cadre réglementaire et de ses particularités. Tout d’abord, l’analyse sociotechnique montre que les conceptions de la santé animale, portées par les éleveurs et les accompagnants techniques spécialisés, sont basés sur une approche préventive et holistique de la santé animale, selon laquelle la gestion de la santé passe avant tout par des leviers liés à la conduite du système d’élevage. Les éleveurs s’entourent d’une diversité d’acteurs pour les accompagner dans cette gestion globale de la santé, qui dépasse le conseil classique du vétérinaire. Ensuite, une autre particularité de la santé en élevage AB traitée ici est relative au statut épidémiologique des élevages : même si une forte variabilité existe entre élevages et pays, l’état sanitaire des troupeaux est en moyenne meilleur en AB qu’en agriculture conventionnelle. Enfin, un focus zootechnique est réalisé sur certaines pratiques d’élevage imposées ou recommandées par le cahier des charges pour des raisons de préservation de la santé ou de la biodiversité. Ces pratiques ont été largement adoptées par les éleveurs en AB et ont eu des impacts positifs sur la préservation de la santé des animaux. Ces particularités ont été traitées dans la littérature de façon inégale selon les filières. Des développements plus importants sont donc faits dans cette synthèse sur les espèces de Ruminants, et des apports plus ponctuels sur les productions porcines et avicoles.
Published: 2022
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8. Porganic, a research organic pig farm: overall presentation and two examples of research programs

Author: Prunier, Arrmelle, Ferchaud, Stéphane, Lebret, Bénédicte, Canario, Laurianne, and Merlot, Elodie
Subjects: Breeding and genetics, Feeding and growth, Health and welfare
Abstract: In a first part, this presentation describes the main characteristics of the Inrae organic pig farm (Porganic) located in Rouillé (86480, France). Secondly, it describes the protocol and results of an experiment performed in Porganic that compares three sources of iron (100 mg dextran iron injected at 4 days of age, soil from the farm distributed from 4 days of age, dry river peat distributed from 4 days of age). Measures concern essentially the haematologic status and suggest that the dry river peat is a very promising solution. This experiment was a part of the Era-Net CORE Organic Cofund project (Power project: https://projects.au.dk/coreorganiccofund/core-organic-cofund-projects/power/). In a third part, this presentation describes the aims and the main measures of two experiments that will be performed in 2022. The aims of these experiments are to develop strategies to prevent undesired behaviours (mounting, aggressions) in intact male pigs and to avoid boar taint (androstenone, skatole) in the end products. Two levels will be studies (genetic within the H2020 PPILOW project (https://www.ppilow.eu/; feeding with the French CASDAR project named Farinelli).
Published: 2021

9. Investigating fallback dietary habits among hominins: perspectives from controlled-feeding experiments on domestic pigs

Author: Louail, Margot, Thiery, Ghislain, Neaux, Dimitri, Ferchaud, Stéphane, Souron, Antoine, Walker, Axelle E.C., SURAULT, JÉRÔME, Merceron, Gildas, Laboratoire de paléontologie, évolution, paléoécosystèmes, paléoprimatologie (PALEVOPRIM ), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Archéozoologie, archéobotanique : sociétés, pratiques et environnements (AASPE), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), De la Préhistoire à l'Actuel : Culture, Environnement et Anthropologie (PACEA), and Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2021

10. Meiotic silencing in pigs: A case study in a translocated azoospermic boar

Author: Mary, Nicolas, Calgaro, Anne, Barasc, Harmonie, Bonnet, Nathalie, Ferchaud, Stéphane, Raymond-Letron, Isabelle, Ducos, Alain, Pinton, Alain, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Expérimentale Elevages Porcins Innovants (GenESI), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), STROMALab, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement Français du Sang-Centre National de la Recherche Scientifique (CNRS), Pinton, Alain, Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Etablissement Français du Sang-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Male, S, MSCI (meiotic sex chromosome inactivation) disturbance, Swine, [SDV]Life Sciences [q-bio], reciprocal translocation, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, QH426-470, MSUC, I, Translocation, Genetic, Article, Pinton, A, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Genetics, Animals, meiosis, Gene Silencing, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Azoospermia, Swine Diseases, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Bonnet, urogenital system, Barasc, Ferchaud, Calgaro, [SDV.BA.MVSA] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, H, N, Chromatin, Ducos, [SDV] Life Sciences [q-bio], Mary, Karyotyping, Raymond-Letron, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], infertility, A. Meiotic Silencing in Pigs: A Case Study in a Translocated infertility
Abstract: Carriers of balanced constitutional reciprocal translocations usually present a normal phenotype, but often show reproductive disorders. For the first time in pigs, we analyzed the meiotic process of an autosome–autosome translocation associated with azoospermia. Meiotic process analysis revealed the presence of unpaired autosomal segments with histone γH2AX accumulation sometimes associated with the XY body. Additionally, γH2AX signals were observed on apparently synapsed autosomes other than the SSC1 or SSC15, as previously observed in Ataxia with oculomotor apraxia type 2 patients or knock-out mice for the Senataxin gene. Gene expression showed a downregulation of genes selected on chromosomes 1 and 15, but no upregulation of SSCX genes. We hypothesized that the total meiotic arrest observed in this boar might be due to the silencing of crucial autosomal genes by the mechanism referred to as meiotic silencing of unsynapsed chromatin (MSUC)., Les porteurs de translocations réciproques constitutionnelles équilibrées présentent généralement un phénotype normal, mais présentent souvent des troubles de la reproduction. Pour la première fois chez le porc, nous avons analysé le processus méiotique d'une translocation autosome-autosome associée à une azoospermie. L'analyse du processus méiotique a révélé la présence de segments autosomiques non appariés avec une accumulation d'histone γH2AX parfois associée à la vésicule sexuelle. De plus, des signaux γH2AX ont été observés sur des autosomes apparemment appariés autres que le SSC1 ou le SSC15, comme cela avait été précédemment observé chez des patients atteints d'Ataxie avec apraxie oculomotrice de type 2 ou chez des souris knock-out pour le gène de la senataxine. L'expression génique a montré une régulation négative des gènes sélectionnés sur les chromosomes 1 et 15, mais aucune régulation positive des gènes du SSCX. Nous avons émis l'hypothèse que l'arrêt méiotique total observé chez ce verrat pourrait être dû à la répression de gènes autosomiques cruciaux par le mécanisme appelé " meiotic silencing of unsynapsed chromatin" (MSUC).
Published: 2021
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11. Investigating the relationship between diet and mandibular morphology: new insights from a controlled-feeding experiment on domestic pigs

Author: Louail, Margot, Neaux, Dimitri, Ferchaud, Stéphane, Merceron, Gildas, Laboratoire de paléontologie, évolution, paléoécosystèmes, paléoprimatologie (PALEVOPRIM ), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Archéozoologie, archéobotanique : sociétés, pratiques et environnements (AASPE), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2021

12. Experimental assessment of the relationship between diet and mandibular morphology using a pig model: New insights for paleodietary reconstructions.

Author: Neaux, Dimitri, Louail, Margot, Ferchaud, Stéphane, Surault, Jérôme, and Merceron, Gildas
Published: 2022
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13. Steroidome And Metabolome Analysis In Saliva From Immature To Pubertal Gilts To Identify Potential Biomarkers Of Receptivity To Boar Effect

Author: Goudet, Ghylène, Liere, Philippe, Pianos, Antoine, Fernandez, Neïké, Cambourg, Annie, Savoie, Jonathan, Staub, Christophe, Venturi, Eric, Douet, Cécile, Ferchaud, Stéphane, Maupertuis, Florence, Roinsard, Antoine, Boulot, Sylviane, and Prunier, Armelle
Subjects: endocrine system, urogenital system, "Organics" in general
Abstract: Our objective was to develop alternatives to hormones for estrus synchronization in gilts. Gilts exhibit a pre-puberty period with high urinary estrone concentration during which boar exposure could induce the first ovulation. We searched for salivary biomarkers of this period. Urine and saliva were collected on six 140-day-old gilts until puberty for estrone assay, metabolome and steroidome analysis. We identified 23 metabolites and 28 steroids in saliva. The concentration of 8 of them showed significant variations at the pre-puberty period, they were candidate biomarkers. Saliva was collected from 30 gilts exposed to a boar and subjected to estrus detection from 150 to 175 days of age. Metabolome and steroidome analyses allowed the identification of 33 metabolites and 29 steroids in saliva. Their concentrations were not significantly different between receptive and non-receptive gilts. Thus, we could not identify salivary biomarkers of the period of receptivity to the boar effect.
Published: 2021

14. Studies of male and female meiosis in inv(4)(p1.4;q2.3) pig carriers

Author: Massip, Katia, Yerle, Martine, Billon, Yvon, Ferchaud, Stéphane, Bonnet, Nathalie, Calgaro, Anne, Mary, Nicolas, Dudez, Anne-Marie, Sentenac, Céline, Plard, Christophe, Ducos, Alain, and Pinton, Alain
Published: 2010
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15. Discriminating dental microwear textures of different seed-eaters: perspectives from a controlled feeding study with pigs

Author: Louail, Margot, Ferchaud, Stéphane, Souron, Antoine, Walker, Axelle E.C., Merceron, Gildas, Laboratoire de paléontologie, évolution, paléoécosystèmes, paléoprimatologie (PALEVOPRIM ), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), De la Préhistoire à l'Actuel : Culture, Environnement et Anthropologie (PACEA), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Louail, Margot, and Stratégies alimentaires et leurs changements enregistrés par les dents fossiles: considérer la séquence évolutive des homininés. - - DIET-Scratches2017 - ANR-17-CE27-0002 - AAPG2017 - VALID
Subjects: [SDV] Life Sciences [q-bio], [SDE.BE] Environmental Sciences/Biodiversity and Ecology, [SDV]Life Sciences [q-bio], [SDU.STU.PG] Sciences of the Universe [physics]/Earth Sciences/Paleontology, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2020

16. Erratum to: Meiotic pairing and gene expression disturbance in germ cells from an infertile boar with a balanced reciprocal autosome-autosome translocation

Author: Barasc, Harmonie, Congras, Annabelle, Mary, Nicolas, Trouilh, Lidwine, Marquet, Valentine, Ferchaud, Stéphane, Raymond-Letron, Isabelle, Calgaro, Anne, Loustau-Dudez, Anne-Marie, Mouney-Bonnet, Nathalie, Acloque, Hervé, Ducos, Alain, and Pinton, Alain
Published: 2016
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17. Steroidome and metabolome analysis in gilt saliva to identify biomarkers of boar effect receptivity

Author: Goudet, Ghylene, Liere, Philippe, Nadal-Desbarats, Lydie, Grivault, Doryan, Douet, Cécile, SAVOIE, Jonathan, Ferchaud, Stéphane, Maupertuis, Florence, ROINSARD, Antoine, BOULOT, Sylviane, Prunier, Armelle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Petites Molécules de neuroprotection, neurorégénération et remyélinisation, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Tours (UT), Génétique, Expérimentation et Système Innovants (GenESI), Institut National de la Recherche Agronomique (INRA), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Chambre Régionale d'Agriculture des Pays de la Loire, Institut Technique de l'Agriculture Biologique (ITAB), Institut du Porc (IFIP), Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Université de Tours
Subjects: [SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], steroid, biomarkers, identification, [INFO]Computer Science [cs]
Abstract: International audience; Our objective was to develop alternatives to hormonal treatments to synchronize oestrus of gilts. Before puberty gilts exhibit a pre-puberty period during which boar exposure could induce and synchronize first ovulation. To develop practical non-invasive tools to identify this period and improve detection of the gilts to stimulate, we searched for salivary biomarkers of the pre-puberty period. Saliva samples were collected from 30 Large-White x Landrace crossbred gilts from 140 to 175 days of age. Gilts were exposed to a boar twice a day and subjected to oestrus detection from 150 to 175 days of age. Among the 30 gilts, 10 were detected in oestrus 4 to 7 days after introduction of the boar and were considered receptive to the boar effect, 14 were detected in oestrus more than 8 days after boar introduction, 6 did not show oestrus and were considered non-receptive. Saliva samples from 6 receptive and 6 non-receptive gilts were analysed for steroidome using GC-MS/MS and for metabolome using 1H-NMR spectroscopy. Four saliva samples per gilt were analysed: 26 days and 11 days before boar introduction (BI-26 and BI-11), the day of boar introduction (BI), 3 days later for receptive gilts (BI+3) or 7 days later for non-receptive gilts (BI+7). Data were analysed using repeated measures one-way ANOVA and orthogonal partial least squares discriminant analysis. Thirty steroids and 35 metabolites were detected in gilt saliva. The concentrations of 6 steroids were higher (P < 0.05) in receptive gilts than in non-receptive gilts at BI-26, BI-11 and BI. The concentration of 2 metabolites were lower (P < 0.05) in receptive gilts than in non-receptive gilts at BI-11. These candidates could be potential salivary biomarkers to detect receptive gilts. However, their low and variable concentrations in saliva require expensive analysis and limit their use in pig farms.
Published: 2019

18. Chromosome rearrangements and meiosis in pig

Author: Pinton, Alain, Mary, Nicolas, Barasc, Harmonie, Bonnet, Nathalie, Calgaro, Anne, Berlib, Pauline, Ferchaud, Stéphane, Letron, Isabelle Raymond, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Génétique, Expérimentation et Système Innovants (GenESI), Institut National de la Recherche Agronomique (INRA), and Université Fédérale Toulouse Midi-Pyrénées
Subjects: pig, genomic, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], genetic
Abstract: Meeting Abstract: L17 (Invité); International audience; Constitutional chromosomal rearrangements are relatively frequent genetic abnormalities in humans and in animal species. They can lead to developmental disorders (malformations, mental retardations), and/ or altered reproduction. In domestic animal species, these rearrangements are potentially responsible for decreased reproductive abilities of the carriers and/or their mates and subsequent economic losses to the animal production industry. Chromosomal rearrangements can be responsible for meiotic division impairments, leading in males to total or partial spermatogenesis arrest (oligo-, azoospermia). Generally, however, heterozygous carriers (male and female) of these abnormalities produce genetically unbalanced gametes, leading to early embryonic mortality. In pigs, this can result in a considerable reduction of the mates’ litter size.
Published: 2019
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19. Testicular development, sex hormones and boar taint in pig lines divergent for residual feed intake

Author: Prunier, Armelle, Billon, Yvon, Ruesche, Julien, Ferchaud, Stéphane, Gilbert, Hélène, Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), UE 1372 Génétique, Expérimentation et Système Innovants, Institut National de la Recherche Agronomique (INRA)-Génétique animale (G.A.)-Physiologie Animale et Systèmes d'Elevage (PHASE), Institut National de la Recherche Agronomique (INRA)-Génétique, Expérimentation et Système Innovants (GenESI), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Génétique, Expérimentation et Système Innovants (GenESI), and Institut National de la Recherche Agronomique (INRA)
Subjects: boar taint, puberty, consommation alimentaire résiduelle, stéroïde, puberte, [SDV]Life Sciences [q-bio], feed efficiency, steroid, efficience alimentaire, porc mâle entier, lignée divergente
Abstract: Improving feed efficiency and rearing entire male pigs are relevant strategies to reduce feed cost and environmental waste in pig production. The major constraint for rearing entire male pigs being boar taint, an experiment was performed to determine the consequences of a divergent selection on residual feed intake (RFI: low RFI = LRFI; high RFI = HRFI) on pubertal development and boar taint. Purebred French Large White male pigs from two divergent lines for RFI (9th generation of selection, n = 45 or 43 pigs/line from 33 litters) were reared in two batches (n = 19 to 24 pigs/line/batch). Blood samples were drawn at 15 and 166±1 days of age (mean±SD) and pigs were weighed. After slaughter at 167±1 days of age, a backfat sample was collected in the neck and the genital tract was removed for testis and epididymis weighing after tissue trimming. Percentages of testis and epididymis weight relatively to liveweight were calculated for statistical analyses. All data were analyzed by ANOVA using R, including line and batch as fixed effects and litter as a random effect. When necessary, a log transformation was applied before analysis and adjusted means were backcalculated. Before slaughter, LRFI pigs were lighter than HRFI pigs (100±2 vs 106±2 kg liveweight, P0.1). However, plasma oestradiol-17 before slaughter (9.0 vs 17.5 pg/ml), fat androstenone (0.22 vs 0.34 µg/g pure fat), testis (3.5 vs 4.4‰) and epidydimis (1.18 vs 1.62‰) relative weights were lower in HRFI than LRFI pigs (P
Published: 2018

20. Divergent selection on adrenocortical activity in Large White pigs: study of responses to breeding challenges

Author: Mormède, Pierre, Terenina, Elena, Billon, Yvon, Ferchaud, Stéphane, Gress, Laure, Rémignon, Hervé, Manse, Hélène, Larzul, Catherine, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Génétique, Expérimentation et Système Innovants (GenESI), and Institut National de la Recherche Agronomique (INRA)
Subjects: pig, stress, [SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, [SDV]Life Sciences [q-bio], selection, [INFO]Computer Science [cs], genetic
Abstract: International audience; This experiment had the objective to analyze responses to various breeding challenges in the third generation of selection of two lines of pigs genetically selected on the basis of plasma cortisol levels measured one hour after injection of ACTH. This test allows to objectify the activity level of the hypothalamic-pituitary-adrenal (HPA) axis, a major neuroendocrine system of the stress response. The results presented here show that the post-weaning growth rate was significantly lower in the high line, although the two lines had the same overall growth rate throughout their productive lives. The effects of a prolonged exposure to high temperature (30°C) and of group mixing stress prior to slaughter did not differ between the lines. These results show that the responses to different challenges are not affected in the same way by the HPA axis activity. These results, together with production data, will allow to evaluate the relevance of this criterion of selection to increase the robustness of animals. With the financial support of the Agence Nationale de la Recherche, program ANR BIOADAPT, project SUSoSTRESS (ANR-12-ADAP-0008). Keywords: genetic selection, cortisol, stress, robustness, heat stress, weaning stress, mixing stress, pig.
Published: 2018

21. A conceptual framework to promote integrated health management in monogastrics

Author: Lamothe, Laurence, Combes, Sylvie, Balmisse, Elodie, Collin, Anne, Ferchaud, Stéphane, Germain, Karine, Pinard - Van Der Laan, Marie-Helene, Schouler, Catherine, Le Floc'h, Nathalie, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], UE 1322 Pôle d'Expérimentation Cunicole TOULousain, Institut National de la Recherche Agronomique (INRA)-Génétique animale (G.A.)-Physiologie Animale et Systèmes d'Elevage (PHASE), Institut National de la Recherche Agronomique (INRA)-Pôle d'Expérimentation Cunicole TOULousain (PECTOUL), Recherches Avicoles (SRA), Institut National de la Recherche Agronomique (INRA), UE 1372 Génétique, Expérimentation et Système Innovants, Institut National de la Recherche Agronomique (INRA)-Génétique, Expérimentation et Système Innovants (GenESI), Elevage Alternatif et Santé des Monogastriques (UE EASM), Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Pôle d'Expérimentation Cunicole TOULousain (PECTOUL ), Unité de Recherches Avicoles (URA), Génétique, Expérimentation et Système Innovants (GenESI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Université de Tours, AGROCAMPUS OUEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)
Subjects: animal health, santé animale, [SDV]Life Sciences [q-bio], integrated management, système d'élevage, gestion intégrée, approche systémique, monogastrique, traitement antimicrobien, pratique d'élevage
Abstract: Rimel; Reduce the use of antimicrobial compounds in livestock production is necessary to limit antimicrobial resistance development and to protect the health of animals, humans and ecosystems. This complex task may only be accomplished via the implementation of a systemic approach, which takes into account the management of animal health as a whole, avoiding simplistic strategies. To that purpose, 15 scientists working on different domestic monogastric species (pig, poultry, rabbit) formed the consortium ‘RIMEL’. We propose an interdisciplinary conceptual framework for the evaluation and management of animal health. This conceptual framework listed 11 fundaments of animal health organized in two main dimensions. The first one, physical health, refers to the association of barriers (skin, mucosae and microbiota) and defenses (innate and acquired immunity, and nervous and endocrine systems). The second one considers the psycho-social health (mother-young interactions or amongst relatives, the expression of the innate behavioral repertoire, and the respect of circadian rhythm). We identified critical periods for health in each species related to the maturation dynamics of each fundament. Then, we associate these fundaments with various indicators of health status (zootechnical performance, behavior, metabolic or hormonal markers, etc.). Finally, the framework takes into account more than 30 practices applied in the long- or in the short-term to drive health in livestock systems (genetics, reproduction strategy and renewal management, feeding, hygiene and prophylaxis, living environment, etc.). We organized all the elements to describe the biological relays, to distinguish levers and risks, and to build a representation that took into account the lag between actions and results on a targetedpopulation. This framework is a tool to (1) help and structure research on integrated management of animal health (2) characterize and analyze existing systems, (3) design and evaluate innovative practices and systems. Our ultimate goal is to support the transition of animal production towards systems with low antimicrobial inputs.
Published: 2017

22. Evaluation of salivary metabolome and steroidome for the identification of biomarkers of the pubertal stage of maturity in gilts

Author: Goudet-Guitton, Ghylène, Nadal-Desbarats, Lydie, Lière, Philippe, Douet, Cécile, SAVOIE, Jonathan, STAUB, Christophe, Venturi, Eric, Ferchaud, Stéphane, Maupertuis, Florence, ROINSARD, Antoine, BOULOT, Sylviane, Prunier, Armelle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Imagerie et cerveau, Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), U 1195, Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Sud (Paris 11), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (Unité Expérimentale de Physiologie Animale de l‘Orfrasiére - UE PAO), Institut National de la Recherche Agronomique (INRA), UE 1372 Génétique, Expérimentation et Système Innovants, Institut National de la Recherche Agronomique (INRA)-Génétique animale (G.A.)-Physiologie Animale et Systèmes d'Elevage (PHASE), Institut National de la Recherche Agronomique (INRA)-Génétique, Expérimentation et Système Innovants (GenESI), Chambre d'agriculture de la Loire Atlantique (CDA), Institut Technique de l'Agriculture Biologique (ITAB), Institut du Porc, Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), Crédit Incitatif PHASE, Agribio4, CIAB, INRA, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Imagerie et cerveau (iBrain - Inserm U1253 - UNIV Tours ), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Génétique, Expérimentation et Système Innovants (GenESI), Institut du Porc (IFIP), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université François Rabelais (Tours). Tours, FRA. Centre National de la Recherche Scientifique (CNRS), FRA., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: pig, saliva, puberty, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, porcine, steroidome, sexual maturity, femelle, maturité sexuelle, biomarker, metabolome, porcin, biomarqueur
Abstract: Evaluation of salivary metabolome and steroidome for the identification of biomarkers of the pubertal stage of maturity in gilts. 2. Journées du GdR 3606 Repro
Published: 2017

23. Sperm resistance to hypotonic stress: comparative analysis among three species of mammals

Author: Tsikis, Guillaume, Ferchaud, Stéphane, Druart, Xavier, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Génétique, Expérimentation et Système Innovants (GenESI), Institut National de la Recherche Agronomique (INRA), International Organisation on Animal Reproduction. FRA., ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract: National audience
Published: 2016

24. Artificial insemination without antibiotics in swine

Author: Ferchaud, Stéphane, BOULOT, Sylviane, Chevrier, Ludivine, Gonzales, Andrés, Schmitt, Eric, Génétique, Expérimentation et Système Innovants (GenESI), Institut National de la Recherche Agronomique (INRA), Institut du Porc (IFIP), and IMV Technologies
Subjects: endocrine system, urogenital system, Insemination artificielle, sang, antibiotique, [SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology, porc, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction
Abstract: National audience; The addition of antibiotics, either alone or in cocktail mixtures, represents today the only way to control bacterial growth during swine semen production and conservation. In general, raw ejaculates are handed at 30-34 °C while delivering and storage of AI doses occur at temperatures around 16-17 °C. However, the risk of inducing bacterial resistance represents a great challenge to overcome. During this study, we set to develop an antibiotic free semen processing technique that at the same time allows for maintenance of sperm quality and does not affect the fertility performance of swine herds. Ejaculates collected from boars of proven fertility were split in two portions and diluted in two different semen extenders. One portion was diluted in a commercial semen extender supplemented with a classical antibiotic mixture and packed in standard semen bags. The second portion was diluted in a new semen extender with no antibiotic addition and packed in bacteriostatic semen bags. Doses were cooled down up to 4°C after packing and kept in a regigerator until inseminations were performed. Computed assisted semen analysis and flow cytometry analysis were carried for evaluating motility, viability and bacterial charge. Further, sixty gilts (n=60) of the cross breed Large-White x Landrace were selected and aleatory grouped. For heat synchronization, eighteen doses of Regumate (20mg) were given daily to the experimental animals. Only gilts found in heat after the synchronization were included in the study. A total of 30 gilts were inseminated with semen doses supplemented with antibiotics and the other 30 with semen doses without antibiotics. Twenty two days after insemination, pregnancy was checked by transabdominal echography, and pregnant gilts were slaughtered for controlling embryo development and number of corpora lutea present in the ovaries at day 30 post insemination. Neither number of embryos, nor ovulation rates (controlled by the number of corpora lutea formed after ovulation) were affected by the antibiotic free AI doses. These results show that antibiotics are no longer necessary for preservation of boar ejaculates as long as minimal hygienic conditions are kept during semen processing.
Published: 2016

25. Search for salivary biomarkers for optimal application of male effect in prepubertal gilts

Author: Goudet, Ghylène, Douet, Cécile, Nadal-Desbarats, Lydie, Staub, Christophe, Venturi, Eric, SAVOIE, Jonathan, Ferchaud, Stéphane, BOULOT, Sylviane, Prunier, Armelle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Institut National de la Recherche Agronomique (INRA), Génétique, Expérimentation et Système Innovants (GenESI), Institut du Porc (IFIP), Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), International Organisation on Animal Reproduction. FRA., and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract: National audience
Published: 2016

26. Adaptation to heat in pig production: the genetic pathway

Author: Gourdine, Jean-Luc, Renaudeau, David, Riquet, Juliette, Billon, Yvon, Ferchaud, Stéphane, Anais, Caroline, Giorgi, Mario, Gilbert, Hélène, and ProdInra, Archive Ouverte
Subjects: [SDV] Life Sciences [q-bio]
Abstract: Heat stressed pigs reduce their feed intake which impair their growth or reproduction performances. Managementsolutions are available to attenuate the effect of heat stress on pigs, such as environmental solutions (water orfeeding management). However, these solutions are technically and economically difficult to implement. Thegenetic selection for improving environmental adaptation in pig production is the most promising long termoption.The PigHeaT project aims 1) at identifying QTLs for heat adaptation, by examining direct responses to find genesinvolved in metabolic ways, indirect responses to find genes affecting growth or robustness to environmentalvariations, 2) at better understanding the physiological mechanisms underlying heat adaptation. The PigHeaTproject (http://www6.inra.fr/pigheat_eng/) is based on original biological resources and original experimentalfacilities. The studied population will be a backcross between Large White pigs, productive but poorlythermotolerant breed, and Creole pigs, low productive but highly thermotolerant breed. The progeny issued fromthis backcross will express all possible levels of thermal tolerance and production performances when submitted toheat stress, depending on the alleles received from their parents. High throughput phenotyping, metabolomics onall the progeny, and transcriptomics on a subset of extreme pigs selected on thermal tolerance response, will beapplied. The design benefits from the unique combination of experimental facilities available at INRA: the firstpart of the project will rely on the backcross population raised in the experimental facilities located in the WestIndies (Guadeloupe, tropical environment). The concomitant production of the same population in theexperimental facilities available in temperate France (Charente Maritime) will allow the detection of genetic byenvironment (GxE) effects for the QTL detected in Guadeloupe.The first phenotypic results confirm the effect of environment on zootechnical and thermoregulatory pigperformance. A significant effect of the production environment has been observed on pig’s growth rate, with adifference of about 100 g / d for the temperate environment (820 vs 720 g / d in the tropics) and lower bodytemperatures (39.4 and 34.8 ° C for rectal and skin temperatures vs. 39.5 and 35.9 ° C in the tropics).
Published: 2015

27. Meiotic recombination analysis for individual chromosomes in male pigs

Author: Mary, Nicolas, Barasc, Harmonie, Ferchaud, Stéphane, Billon, Yvon, Meslier, Frederic, Robelin, David, Calgaro, Anne, Dudez, Anne-Marie, Bonnet, Nathalie, Yerle, Martine, Ducos, Alain, Pinton, Alain, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Génétique, Expérimentation et Système Innovants (GenESI), Institut National de la Recherche Agronomique (INRA), and Centre National de la Recherche Scientifique (CNRS)
Subjects: pig, [SDV]Life Sciences [q-bio], meiotic, [INFO]Computer Science [cs], genetic, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2014
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28. Analysis of male infertility: a case study in pigs

Author: Barasc, Harmonie, Mary, Nicolas, Ducos, Alain, Ferchaud, Stéphane, Raymond Letron, Isabelle, Yerle, Martine, ACLOQUE, HERVE, Pinton, Alain, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Génétique, Expérimentation et Système Innovants (GenESI), Institut National de la Recherche Agronomique (INRA), and Ecole Nationale Vétérinaire de Toulouse (ENVT)
Subjects: pig, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], genetic, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2014
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29. Sperm DNA Methylation Analysis in Swine Reveals Conserved and Species-Specific Methylation Patterns and Highlights an Altered Methylation at the GNAS Locus in Infertile Boars

Author: CONGRAS, Annabelle, Yerle-Bouissou, Martine, Pinton, Alain, Vignoles, Florence, Liaubet, Laurence, Ferchaud, Stéphane, ACLOQUE, HERVE, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Génétique, Expérimentation et Système Innovants (GenESI), and Institut National de la Recherche Agronomique (INRA)
Subjects: gnas, pig, endocrine system, urogenital system, [SDV]Life Sciences [q-bio], imprinted gene, dna methylation, swine, [INFO]Computer Science [cs], infertility, sperm
Abstract: "Chantier qualité spécifique "Auteurs Externes" département de Génétique animale : uniquement liaison auteur au référentiel HR-Access "; International audience; Male infertility is an increasing health issue in today's society for both human and livestock populations. In livestock, male infertility slows the improvement of animal selection programs and agricultural productivity. There is increasing evidence that epigenetic marks play an important role in the production of good-quality sperm. We therefore screened for specific or common epigenetic signatures of livestock infertility. To do so, we compared DNA methylation level in sperm DNA from fertile and infertile boars. We evaluated first the global level of sperm DNA methylation and found no difference between the two groups of boars. We then selected 42 loci of interest, most of them known to be imprinted in human or mice, and assessed the imprinting status of five of them not previously described in swine tissues: WT1, CNTN3, IMPACT, QPCT, and GRB10. DNA methylation level was then quantified in fertile and infertile boars at these 42 loci. Results from fertile boars indicated that the methylation level of the selected loci is highly conserved between pig, human, and mice, with a few exceptions, including the POU5F1 (OCT4) promoter and RTL1. Comparison between fertile and infertile boars revealed that one imprinted region, the GNAS locus, shows an increase in sperm DNA methylation in three out of eight infertile boars with low semen quality. This increase in DNA methylation is associated with an altered expression of the genes belonging to the GNAS locus, suggesting a new role for GNAS in the proper formation of functional gametes.
Published: 2014
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30. Intraindividual Variation of Meiotic Recombination Parameters in Pig Spermatocytes: A Preliminary Study.

Author: Mary, Nicolas, Ferchaud, Stéphane, Barasc, Harmonie, Calgaro, anne, Bonnet, Nathalie, Ducos, alain, and Pinton, alain
Subjects: *SYNAPTONEMAL complexes, *CYTOLOGICAL techniques, *MEIOSIS, *SPERMATOZOA, *GENETIC recombination
Abstract: Meiotic recombination parameters like crossover (CO) rate or synaptonemal complex (SC) length are known to vary strongly between individuals and between cells from the same individual. The origins of this variability remain elusive, and little is known about the variations that might occur between different samples and/or over time within the same individual. To document this question, pachytene cells from 3 boars of the Large White breed were analyzed twice, at a 1-year interval, using immunocytological techniques. CO rate, SC length, and MLH1 inter-foci distances varied significantly between the 3 individuals. CO rate and SC length differed significantly between the 2 sampling periods for 1 individual. However, no significant differences were observed between the 2 samples for CO distribution and inter-foci distances in the 3 boars studied. [ABSTRACT FROM AUTHOR]
Published: 2018
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31. A 5 year retrospective analysis of the rate of mount refusals among AI-boars

Author: Ferchaud, Stéphane, Furstoss, Vincent, Boutin, Jany, Boulot, S., Insémination Caprine et Porcine (ICP), Institut National de la Recherche Agronomique (INRA), Institut du Porc (IFIP), and European Society for Domestic Animal Reproduction (ESDAR). INT.
Subjects: libido, race porcine pietrain, race porcine landrace, [SDV]Life Sciences [q-bio], race porcine large white, insémination artificielle, lignée génétique, collecte de sperme, âge, modèle linéaire, [INFO]Computer Science [cs], verrat, accouplement, ComputingMilieux_MISCELLANEOUS, porc
Abstract: International audience
Published: 2012
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32. Seminal plasma-spermatozoa interactions in the boar

Author: Gérard, Nadine, Gaudin, Marie, Labas, Valérie, Teixeira-Gomes, Ana-Paula, Tsikis, Guillaume, Ferchaud, Stéphane, Druart, Xavier, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Insémination Caprine et Porcine (ICP), Institut National de la Recherche Agronomique (INRA), European Society for Domestic Animals. Dublin, IRL., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)
Subjects: [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2012
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33. Twenty-year trends in AI-boar longevity and reasons for early culling

Author: Furstoss, Vincent, Ferchaud, Stéphane, Boulot, S., Renaud, Guy, Guillouet, Philippe, Insémination Caprine et Porcine (ICP), Institut National de la Recherche Agronomique (INRA), Institut du Porc (IFIP), and European Societu for Domestic Animal Reproduction (ESDAR).
Subjects: [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2012
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34. Meiotic analysis of a teratospermic boar

Author: Barasc, Harmonie, Mary, Nicolas, Ferchaud, Stéphane, Letron, Raymond, Calgaro, Anne, Dudez, Anne-Marie, Bonnet, Nathalie, Bouissou-Matet Yerle, Martine, Ducos, Alain, Pinton, Alain, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Insémination Caprine et Porcine (ICP), Institut National de la Recherche Agronomique (INRA), and Ecole Nationale Vétérinaire de Toulouse (ENVT)
Subjects: [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2012

35. Adaptation to heat in pig production : the genetic pathway

Author: Gourdine, Jean-Luc, Gilbert, Hélène, Riquet, Juliette, Renaudeau, David, Bidanel, Jean Pierre, San Cristobal, Magali, Liaubet, Laurence, Labrune, Yann, Gress, Laure, Feve, Katia, Villa-Vialaneix, Nathalie, Fleury, Jérôme, Anais, Caroline, Giorgi, Mario, Silou, Félix, Bructer, Mélain, Bocage, Bruno, Bénony, Katia, Beramice, David, Billon, Yvon, Bailly, Jean, Gerbe, Philippe, Meslier, Frederic, Epagneaud, Philippe, Le Bourhis, Christophe, Ferchaud, Stéphane, Staub, Christophe, Marie-Magdeleine, Carine, Philibert, Lucien, Unité de Recherches Zootechniques (URZ), Institut National de la Recherche Agronomique (INRA), Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Université Paris 1 Panthéon-Sorbonne (UP1), Plateforme Tropicale d'Expérimentation sur l'Animal (PTEA), Génétique Expérimentale en Productions Animales (GEPA), Insémination Caprine et Porcine (ICP), Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), INRA, ANR-12-ADAP-0015,Pig Heat Tolerance PigHeaT,Adaptation des porcs à la chaleur: la voie génétique(2012), Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Panthéon-Sorbonne (UP1), UE 1297 Unité Expérimentale de Physiologie Animale de l'Orfrasière, Institut National de la Recherche Agronomique (INRA)-Physiologie Animale et Systèmes d'Elevage (PHASE), Institut National de la Recherche Agronomique (INRA)-Unité Expérimentale de Physiologie Animale de l'Orfrasière (UE PAO), and ANR
Subjects: pig, heat stress, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, genetic by environment interaction, climate change, transcriptomic, genetic, QTLs, integrated analysis, metabolomic
Abstract: "Chantier qualité spécifique "Auteurs Externes" département de Génétique animale : uniquement liaison auteur au référentiel HR-Access "; il s'agit d'un type de produit dont les métadonnées ne correspondent pas aux métadonnées attendues dans les autres types de produit : REPORT; The climate is changing and according to the recent estimates from the IPCC, the likelihood of heatwave events is expected to increase both in number and in intensity. Temperature is projected toincrease from 1.8 to 4.0°C from 1980-1999 to 2090-2099. Hence, heat stress-related costs in pigproduction will be amplified in the future, both in temperate areas (summer heat waves) and tropicalareas (hot and humid environment). Meanwhile, world pig production is moving rapidly to tropicaland subtropical regions reaching now more than 50% of the total production. The world developmentof pig production has been achieved through improvement of animal genetics and management intemperate countries. However, selection performed in optimally controlled conditions has increasedthe sensitivity of animals to high ambient temperature. Heat stressed pigs reduce their feed intakewhich impair their growth or reproduction performances. Management solutions are available toattenuate the effect of heat stress on pigs, such as environmental solutions (water or feedingmanagement). However, these solutions are technically and economically difficult to implement. Thegenetic selection for improving environmental adaptation in pig production is the most promising longterm option. The PigHeaT project aims 1) at identifying QTLs for heat adaptation, by examining directresponses to find genes involved in metabolic ways, indirect responses to find genes affecting growthor robustness to environmental variations, 2) at better understanding the physiological mechanismsunderlying heat adaptation. It will provide tools for improving breeding strategies to face theupcoming global warming, and knowledge to better comprehend the physiological reactions ofanimals submitted to short and long term heat stress. The PigHeaT project is based on originalbiological resources and original experimental facilities. The studied population will be a backcrossbetween Large White pigs, productive but poorly thermotolerant breed, and Creole pigs, lowproductive but highly thermotolerant breed. The progeny issued from this backcross will express allpossible levels of thermal tolerance and production performances when submitted to heat stress,depending on the alleles received from their parents. High throughput phenotyping, metabolomics onall the progeny, and transcriptomics on a subset of extreme pigs selected on thermal toleranceresponse, will be applied. It will allow to refine the phenotypes and to achieve a high level of accuracyin QTL detection in the frame of the PigHeaT project. Additionally, the design will benefit from theunique combination of experimental facilities available at INRA: the first part of the project will relyon the backcross population raised in the experimental facilities located in the West Indies(Guadeloupe, tropical environment). The concomitant production of the same population in theexperimental facilities available in temperate France (Charente Maritime) will allow the detection ofgenetic by environment (GxE) effects for the QTL detected in Guadeloupe. Moreover, a heat wavephenomenon will be systematically simulated in the temperate environment at the end of the growingperiod. As a result, chromosomal regions robust or susceptible to GxE interactions will be identified,GxE being either tropical vs temperate, or tropical vs heat wave. Finally, an integrated analysis of the(fine) phenotypes and QTL will be proposed to better understand the metabolic pathways involved inheat stress responses. The respective use of the QTL and biological knowledge in further breedingstrategies will finally be considered.
Published: 2012

36. DNA methylation analysis in sperm from infertile/subfertile boars

Author: ACLOQUE, HERVE, Pinton, Alain, CONGRAS, Annabelle, Vignoles, Florence, Delcros, Chantal, Ferchaud, Stéphane, Riquet, Juliette, Bouissou Matet Yerle, Martine, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Insémination Caprine et Porcine (ICP), and Institut National de la Recherche Agronomique (INRA)
Subjects: [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], methylation, dna, infertility, epigenetic, sperm, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2012

37. Post-epididymal maturation of boar spermatozoa

Author: Dewaele, Laure, Tsikis, Guillaume, Ferchaud, Stéphane, Labas, Valérie, Spina, Lucie, Teixeira-Gomes, Ana-Paula, Druart, Xavier, Gérard, Nadine, ProdInra, Migration, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Insémination Caprine et Porcine (ICP), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDV.SA] Life Sciences [q-bio]/Agricultural sciences, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2011

38. Fertility and prolificacy following ovulation induction (by PG600 or hCG) and a single fixed time AI

Author: Swarts, H.M.J.M., Driancourt, Marc Antoine, Ferchaud, Stéphane, Guillouet, Philippe, Pere, K., Intervet/Schering-Plough Animal Health, Insémination Caprine et Porcine (ICP), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration
Subjects: [SDV] Life Sciences [q-bio], INSEMINATION ARTICIELLE, ARTIFICIAL INSEMINATION, OVULATION INDUCTION, PROLIFICITY, [SDV]Life Sciences [q-bio], FERTILITY, [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2010

39. Seminal plasma and spermatozoa quality in the boar: identification of novel biomarkers

Author: Dewaele, Laure, Tsikis, Guillaume, Ferchaud, Stéphane, Labas, Valérie, Spina, Lucie, Teixeira, Ana Paula, Druart, Xavier, Gérard, Nadine, ProdInra, Migration, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Insémination Caprine et Porcine (ICP), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], CYTOMETRIE EN FLUX, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2010

40. Meiotic Recombination Analyses in Pigs Carrying Different Balanced Structural Chromosomal Rearrangements.

Author: Mary, Nicolas, Barasc, Harmonie, Ferchaud, Stéphane, Priet, Aurélia, Calgaro, Anne, Loustau-Dudez, Anne-Marie, Bonnet, Nathalie, Yerle, Martine, Ducos, Alain, and Pinton, Alain
Subjects: MEIOSIS, CHROMOSOMAL rearrangement, GENETIC recombination, GENETIC carriers, CHROMOSOMAL translocation, LABORATORY swine
Abstract: Correct pairing, synapsis and recombination between homologous chromosomes are essential for normal meiosis. All these events are strongly regulated, and our knowledge of the mechanisms involved in this regulation is increasing rapidly. Chromosomal rearrangements are known to disturb these processes. In the present paper, synapsis and recombination (number and distribution of MLH1 foci) were studied in three boars (Sus scrofa domestica) carrying different chromosomal rearrangements. One (T34he) was heterozygote for the t(3;4)(p1.3;q1.5) reciprocal translocation, one (T34ho) was homozygote for that translocation, while the third (T34Inv) was heterozygote for both the translocation and a pericentric inversion inv(4)(p1.4;q2.3). All three boars were normal for synapsis and sperm production. This particular situation allowed us to rigorously study the impact of rearrangements on recombination. Overall, the rearrangements induced only minor modifications of the number of MLH1 foci (per spermatocyte or per chromosome) and of the length of synaptonemal complexes for chromosomes 3 and 4. The distribution of MLH1 foci in T34he was comparable to that of the controls. Conversely, the distributions of MLH1 foci on chromosome 4 were strongly modified in boar T34Inv (lack of crossover in the heterosynaptic region of the quadrivalent, and crossover displaced to the chromosome extremities), and also in boar T34ho (two recombination peaks on the q-arms compared with one of higher magnitude in the controls). Analyses of boars T34he and T34Inv showed that the interference was propagated through the breakpoints. A different result was obtained for boar T34ho, in which the breakpoints (transition between SSC3 and SSC4 chromatin on the bivalents) seemed to alter the transmission of the interference signal. Our results suggest that the number of crossovers and crossover interference could be regulated by partially different mechanisms. [ABSTRACT FROM AUTHOR]
Published: 2016
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41. Meiotic Recombination Analyses of Individual Chromosomes in Male Domestic Pigs (Sus scrofa domestica).

Author: Mary, Nicolas, Barasc, Harmonie, Ferchaud, Stéphane, Billon, Yvon, Meslier, Frédéric, Robelin, David, Calgaro, Anne, Loustau-Dudez, Anne-Marie, Bonnet, Nathalie, Yerle, Martine, Acloque, Hervé, Ducos, Alain, and Pinton, Alain
Subjects: MEIOSIS, RECOMBINANT DNA, TELOMERES, SEX chromosomes, HOMOLOGOUS chromosomes, CELL cycle, SYNAPTONEMAL complexes
Abstract: For the first time in the domestic pig, meiotic recombination along the 18 porcine autosomes was directly studied by immunolocalization of MLH1 protein. In total, 7,848 synaptonemal complexes from 436 spermatocytes were analyzed, and 13,969 recombination sites were mapped. Individual chromosomes for 113 of the 436 cells (representing 2,034 synaptonemal complexes) were identified by immunostaining and fluorescence in situ hybridization (FISH). The average total length of autosomal synaptonemal complexes per cell was 190.3 µm, with 32.0 recombination sites (crossovers), on average, per cell. The number of crossovers and the lengths of the autosomal synaptonemal complexes showed significant intra- (i.e. between cells) and inter-individual variations. The distributions of recombination sites within each chromosomal category were similar: crossovers in metacentric and submetacentric chromosomes were concentrated in the telomeric regions of the p- and q-arms, whereas two hotspots were located near the centromere and in the telomeric region of acrocentrics. Lack of MLH1 foci was mainly observed in the smaller chromosomes, particularly chromosome 18 (SSC18) and the sex chromosomes. All autosomes displayed positive interference, with a large variability between the chromosomes. [ABSTRACT FROM AUTHOR]
Published: 2014
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42. Corrigendum: Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells.

Author: Congras, Annabelle, Barasc, Harmonie, Canale-Tabet, Kamila, Plisson-Petit, Florence, Delcros, Chantal, Feraud, Olivier, Oudrhiri, Noufissa, Hadadi, Eva, Griscelli, Franck, Bennaceur-Griscelli, Annelise, Turhan, Ali, Afanassieff, Marielle, Ferchaud, Stéphane, Pinton, Alain, Yerle-Bouissou, Martine, and Acloque, Hervé
Abstract: This corrects the article DOI: 10.1038/srep27059 [ABSTRACT FROM AUTHOR]
Published: 2018
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43. Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells.

Author: Congras, Annabelle, Barasc, Harmonie, Canale-Tabet, Kamila, Plisson-Petit, Florence, Delcros, Chantal, Feraud, Olivier, Oudrhiri, Noufissa, Hadadi, Eva, Griscelli, Franck, Bennaceur-Griscelli, Annelise, Turhan, Ali, Afanassieff, Marielle, Ferchaud, Stéphane, Pinton, Alain, Yerle-Bouissou, Martine, and Acloque, Hervé
Published: 2016
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44. Cytogenetic analysis of somatic and germinal cells from 38,XX/38,XY phenotypically normal boars.

Author: Barasc, Harmonie, Ferchaud, Stéphane, Mary, Nicolas, Cucchi, Marie Adélaïde, Lucena, Amalia Naranjo, Raymond Letron, Isabelle, Calgaro, Anne, Bonnet, Nathalie, Dudez, Anne Marie, Yerle, Martine, Ducos, Alain, and Pinton, Alain
Subjects: *CYTOGENETICS, *SOMATIC cells, *GERM cells, *BOARS, *PHENOTYPES, *CHROMOSOME abnormalities, *REPRODUCTION
Abstract: Abstract: Many chromosomal abnormalities have been reported to date in pigs. Most of them have been balanced structural rearrangements, especially reciprocal translocations. A few cases of XY/XX chimerism have also been diagnosed within the national systematic chromosomal control program of young purebred boars carried out in France. Until now, this kind of chromosomal abnormality has been mainly reported in intersex individuals. We investigated 38,XY/38,XX boars presenting apparently normal phenotypes to evaluate the potential effects of this particular chromosomal constitution on their reproductive performance. To do this, we analyzed (1) the chromosomal constitution of cells from different organs in one boar; (2) the aneuploidy rates for chromosomes X, Y, and 13 in sperm nuclei sampled from seven XY/XX boars. 2n = 38,XX cells were identified in different nonhematopoietic tissues including testis (frequency, <8%). Similar aneuploidy rates were observed in the sperm nuclei of XY/XX and normal individuals (controls). Altogether, these results suggest that the presence of XX cells had no or only a very limited effect on the reproduction abilities of the analyzed boars. [Copyright &y& Elsevier]
Published: 2014
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45. Meiotic Recombination Analyses of Individual Chromosomes in Male Domestic Pigs (Sus scrofa domestica).

Author: Mary, Nicolas, Barasc, Harmonie, Ferchaud, Stéphane, Billon, Yvon, Meslier, Frédéric, Robelin, David, Calgaro, Anne, Loustau-Dudez, Anne-Marie, Bonnet, Nathalie, Yerle, Martine, Acloque, Hervé, Ducos, Alain, and Pinton, Alain
Subjects: *MEIOSIS, *RECOMBINANT DNA, *TELOMERES, *SEX chromosomes, *HOMOLOGOUS chromosomes, *CELL cycle, *SYNAPTONEMAL complexes
Abstract: For the first time in the domestic pig, meiotic recombination along the 18 porcine autosomes was directly studied by immunolocalization of MLH1 protein. In total, 7,848 synaptonemal complexes from 436 spermatocytes were analyzed, and 13,969 recombination sites were mapped. Individual chromosomes for 113 of the 436 cells (representing 2,034 synaptonemal complexes) were identified by immunostaining and fluorescence in situ hybridization (FISH). The average total length of autosomal synaptonemal complexes per cell was 190.3 µm, with 32.0 recombination sites (crossovers), on average, per cell. The number of crossovers and the lengths of the autosomal synaptonemal complexes showed significant intra- (i.e. between cells) and inter-individual variations. The distributions of recombination sites within each chromosomal category were similar: crossovers in metacentric and submetacentric chromosomes were concentrated in the telomeric regions of the p- and q-arms, whereas two hotspots were located near the centromere and in the telomeric region of acrocentrics. Lack of MLH1 foci was mainly observed in the smaller chromosomes, particularly chromosome 18 (SSC18) and the sex chromosomes. All autosomes displayed positive interference, with a large variability between the chromosomes. [ABSTRACT FROM AUTHOR]
Published: 2014
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46. Evolution of steroid concentrations in saliva from immature to pubertal gilts for the identification of biomarkers of gilts receptivity to boar effect.

Author: Goudet, Ghylène, Liere, Philippe, Pianos, Antoine, Fernandez, Neïké, Cambourg, Annie, Savoie, Jonathan, Staub, Christophe, Venturi, Eric, Douet, Cécile, Ferchaud, Stéphane, Maupertuis, Florence, Roinsard, Antoine, Boulot, Sylviane, and Prunier, Armelle
Subjects: *SALIVA, *SOWS, *TANDEM mass spectrometry, *BOARS, *SWINE, *ANIMAL welfare, *HYDROCORTISONE
Abstract: • Steroidome analysis allowed quantifying 28 steroids in gilts saliva. • Steroids concentrations in gilts saliva varied from immature to pubertal stage. • We identified steroids that could be biomarkers of the receptivity to boar effect. • We contributed to the development of non-hormonal tools for estrous synchronization. • Non-invasive saliva sampling is in agreement with improvement of animal welfare. Estrus synchronization is necessary for management of gilt reproduction in pig farms. It is usually achieved by using synthetic progestagens, but there is increasing demand for non-hormonal alternative tools with the prospect of sustainability of livestock production. Moreover, in organic farms, synthetic hormones are not allowed. Before reaching puberty, gilts exhibit a "waiting period" during which external stimulations, such as boar exposure, could trigger and synchronize the first ovulation. However, practical non-invasive tools for detection of the "waiting period" in pig farms are lacking. During this period, estrone levels in urine are high, but urine sampling is difficult in group-housed females. Our objective was to identify among steroids potential biomarkers of this "waiting period" through saliva monitoring from immature to pubertal gilts using gas chromatography coupled to tandem mass spectrometry. Starting between 144 and 147 days of age, six Large White gilts were submitted to ultrasound puberty diagnosis 3 times a week until first ovulation. Urine and saliva samples were collected to analyze weekly estrone and steroidome respectively, until puberty. Urinary estrone concentration significantly increased 2 weeks before first ovulation occurring between 182 and 192 days of age. The period with increasing estrone levels was considered as the "waiting period". Steroidome analysis allowed identifying and quantifying 28 steroids in 500 µl of gilts saliva. Significant decrease of dehydroepiandrosterone and significant increase of 5α-dihydroprogesterone and 17β-estradiol were detected 2 weeks before puberty, suggesting that these steroids could be potential biomarkers of the "waiting period". These results show that painless sampling of saliva could be a non-invasive welfare-friendly tool for the identification of the physiological hormonal status of the gilts and possibly the optimal time for application of the boar effect, a solution to synchronize puberty without exogenous hormones. [ABSTRACT FROM AUTHOR]
Published: 2019
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47. Experimental assessment of the relationship between diet and mandibular morphology using a pig model: New insights for paleodietary reconstructions.

Author: Neaux D, Louail M, Ferchaud S, Surault J, and Merceron G
Subjects: Animals, Edible Grain chemistry, Mammals, Mandible anatomy & histology, Mastication, Swine, Diet, Hominidae
Abstract: Dietary habits exert significant selective pressures on anatomical structures in animals, leading to substantial morphological adaptations. Yet, the relationships between the mandible and diet are still unclear, raising issues for paleodietary reconstructions notably. To assess the impact of food hardness and size on morphological structures, we used an experimental baseline using a model based on the domestic pig, an omnivorous mammal with bunodont, thick-enameled dentition, and chewing movements similar to hominids. We hypothesized that the consumption of different types of seeds would result in substantial differences in the morphology of the mandible despite similar overall diets. The experiment was conducted on four groups of juvenile pigs fed with mixed cereal and soy flours. The control group received only flours. We supplemented the four others with either 10 hazelnuts, 30 hazelnuts, 30% barley seeds, or 20% corn kernels per day. We investigated the shape differences between the controlled-fed groups using three-dimensional geometric morphometrics. Our results provide strong evidence that the supplemental consumption of a significant amount of seeds for a short period (95 days) substantially modify the mandibular morphology of pigs. Our analyses suggest that this shape differentiation is due to the size of the seeds, requiring high and repeated bite force, rather than their hardness. These results provide new perspectives for the use of mandibular morphology as a proxy in paleodietary reconstructions complementing dental microwear textures analyses., (© 2022 American Association for Anatomy.)
Published: 2022
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48. Meiotic Silencing in Pigs: A Case Study in a Translocated Azoospermic Boar.

Author: Mary N, Calgaro A, Barasc H, Bonnet N, Ferchaud S, Raymond-Letron I, Ducos A, and Pinton A
Subjects: Animals, Azoospermia genetics, Chromatin genetics, Karyotyping, Male, Azoospermia veterinary, Gene Silencing, Meiosis genetics, Swine genetics, Swine Diseases genetics, Translocation, Genetic
Abstract: Carriers of balanced constitutional reciprocal translocations usually present a normal phenotype, but often show reproductive disorders. For the first time in pigs, we analyzed the meiotic process of an autosome-autosome translocation associated with azoospermia. Meiotic process analysis revealed the presence of unpaired autosomal segments with histone γH2AX accumulation sometimes associated with the XY body. Additionally, γH2AX signals were observed on apparently synapsed autosomes other than the SSC1 or SSC15, as previously observed in Ataxia with oculomotor apraxia type 2 patients or knock-out mice for the Senataxin gene. Gene expression showed a downregulation of genes selected on chromosomes 1 and 15, but no upregulation of SSCX genes. We hypothesized that the total meiotic arrest observed in this boar might be due to the silencing of crucial autosomal genes by the mechanism referred to as meiotic silencing of unsynapsed chromatin (MSUC).
Published: 2021
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49. Lactation and gestation controls on calcium isotopic compositions in a mammalian model.

Author: Hassler A, Martin JE, Ferchaud S, Grivault D, Le Goff S, Albalat E, Hernandez JA, Tacail T, and Balter V
Subjects: Animals, Animals, Newborn, Body Fluids chemistry, Female, Pregnancy, Swine, Animal Feed analysis, Body Fluids metabolism, Bone and Bones chemistry, Calcium metabolism, Calcium Isotopes analysis, Lactation, Models, Biological
Abstract: Lactation and gestation are among the physiological events that trigger the most intense changes in body calcium (Ca) fluxes. Along with the composition of the animal 2021 diet, these events are suspected to impact the Ca isotopic composition of Ca body reservoirs but their dynamics are poorly understood. In this study, we monitored a group of domestic sows across a full reproduction cycle. We collected tissues and fluids (blood, urine, milk, colostrum, umbilical blood, adult and piglet bones) at different steps of gestation and lactation, and analyzed their Ca isotopic compositions (i.e. δ44/42Ca) by means of multi-collector inductively coupled plasma mass spectrometry. Among other results, we report the first observations of Ca isotopic fractionation between maternal and umbilical blood (Δ44/42Caumbilical blood-sow blood = -0.18 ± 0.11‰, n = 3). Our data also highlight that gestation and lactation periods are characterized by small diet-bone Ca isotopic offsets (Δ44/42Cabone-diet = -0.28 ± 0.11‰, n = 3), with 44Ca-enriched blood compositions during nursing (Δ44/42Canursing blood-gestation blood = $+ 0.42{\rm{\,\,}}_{ - 0.12}^{ + 0.11}$‰, n = 3). Under the light of an up-to-date mammalian box model, we explored different scenarios of gestation and lactation Ca fluxes experienced by a sow-like animal. These simulations suggest that gestation changes on body δ44/42Ca values may result from the intensification of Ca absorption by the animal, whereas the production of 44Ca-depleted milk is the main driver for the 44Ca enrichment in blood during lactation. In addition, our results also support that bone mineralization could be associated with a more restricted Ca isotopic fractionation than previously envisioned. Together, these results refine the framework of Ca isotope applications, notably regarding the monitoring of human bone balance and the study of species and ecosystems from the present and the past., (© The Author(s) 2021. Published by Oxford University Press.)
Published: 2021
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50. Sperm DNA methylation analysis in swine reveals conserved and species-specific methylation patterns and highlights an altered methylation at the GNAS locus in infertile boars.

Author: Congras A, Yerle-Bouissou M, Pinton A, Vignoles F, Liaubet L, Ferchaud S, and Acloque H
Subjects: Animals, Conserved Sequence, Epigenesis, Genetic, Female, Genetic Loci, Genomic Imprinting, Infertility, Male metabolism, Male, Pregnancy, Semen Analysis, Species Specificity, DNA Methylation, GTP-Binding Protein alpha Subunits, Gs genetics, Gene Expression Profiling, Infertility, Male genetics, Spermatozoa metabolism, Swine genetics
Abstract: Male infertility is an increasing health issue in today's society for both human and livestock populations. In livestock, male infertility slows the improvement of animal selection programs and agricultural productivity. There is increasing evidence that epigenetic marks play an important role in the production of good-quality sperm. We therefore screened for specific or common epigenetic signatures of livestock infertility. To do so, we compared DNA methylation level in sperm DNA from fertile and infertile boars. We evaluated first the global level of sperm DNA methylation and found no difference between the two groups of boars. We then selected 42 loci of interest, most of them known to be imprinted in human or mice, and assessed the imprinting status of five of them not previously described in swine tissues: WT1, CNTN3, IMPACT, QPCT, and GRB10. DNA methylation level was then quantified in fertile and infertile boars at these 42 loci. Results from fertile boars indicated that the methylation level of the selected loci is highly conserved between pig, human, and mice, with a few exceptions, including the POU5F1 (OCT4) promoter and RTL1. Comparison between fertile and infertile boars revealed that one imprinted region, the GNAS locus, shows an increase in sperm DNA methylation in three out of eight infertile boars with low semen quality. This increase in DNA methylation is associated with an altered expression of the genes belonging to the GNAS locus, suggesting a new role for GNAS in the proper formation of functional gametes., (© 2014 by the Society for the Study of Reproduction, Inc.)
Published: 2014
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