36 results on '"Figliolini, F."'
Search Results
2. MICROVESICLES DERIVED FROM ENDOTHELIAL PROGENITOR CELLS PROTECT KIDNEYS AND PANCREATIC ISLETS FROM ISCHEMIA-REPERFUSION INJURY: O115 (F.12-5)
- Author
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Figliolini, F., Cantaluppi, V., Medica, D., Beltramo, S., Gatti, S., Bruno, S., De Lena, M., Tetta, C., and Camussi, G.
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- 2012
3. Isolation, Characterization and Pro-Angiogenic Role of Microvesicles Derived from Human Pancreatic Islets.: Abstract# 1533 Poster Board #-Session: P95-IV
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Cantaluppi, V., Figliolini, F., Beltramo, S., Delena, M., Biancone, L., Segoloni, G. P., and Camussi, G.
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- 2012
4. Microvesicles Derived from Endothelial Progenitor Cells Protect from Antibody- and Complement-Mediated Endothelial Injury through the Horizontal Transfer of Specific mRNAs and microRNAs.: Abstract# 78
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Cantaluppi, V., Delena, M., Medica, D., Figliolini, F., Beltramo, S., Tognarelli, G., Biancone, L., Segoloni, G. P., and Camussi, G.
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- 2012
5. Antiangiogenic and Immunomodulatory Effects of Rapamycin on Islet Endothelium: Relevance for Islet Transplantation
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Cantaluppi, V., Biancone, L., Romanazzi, G. Mauriello, Figliolini, F., Beltramo, S., Ninniri, M. S., Galimi, F., Romagnoli, R., Franchello, A., Salizzoni, M., Perin, P. Cavallo, Ricordi, C., Segoloni, G. P., and Camussi, G.
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- 2006
6. Inclusions of second quantization algebras
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Figliolini, F and Guido, D
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Settore MAT/05 - Analisi Matematica - Published
- 2000
7. On the type of second quantization factors
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Figliolini, F and Guido, D
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Settore MAT/05 - Analisi Matematica - Published
- 1994
8. The Tomita operator for the free scalar field
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Figliolini, F and Guido, D
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Settore MAT/05 - Analisi Matematica - Published
- 1989
9. Infrared representations of the free Bose fields
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Doplicher, S, Figliolini, F, and Guido, D
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Settore MAT/05 - Analisi Matematica - Published
- 1984
10. PROTECTIVE ROLE OF MACROPHAGE STIMULATING PROTEIN ON RENAL TUBULAR EPITHELIAL CELLS: RELEVANCE FOR REGENERATION AFTER DELAYED KIDNEY GRAFT FUNCTION.
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Cantaluppi, V, Figliolini, F, Beltramo, S, Romanazzi, G M., Medica, D, Migliori, M, Mannari, C, Biancone, L, Segoloni, G P., and Camussi, G
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- 2008
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11. THROMBOSPONDIN-1 (TSP-1) MEDIATES THE ANTI ANGIOGENIC AND IMMUNOMODULATORY EFFECTS OF RAPAMYCIN ON PANCREATIC ISLET ENDOTHELIAL CELLS: RELEVANCE FOR ISLET TRANSPLANTATION.
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Cantaluppi, V, Beltramo, S, Figliolini, F, Romanazzi, G M., Biancone, L, Segoloni, G P., and Camussi, G
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- 2008
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12. Plasma NGAL Is An Early Biomarker of Graft Function, Calcineurin Inhibitor Nephrotoxicity and Tubular Regeneration in Kidney Transplantation from Extended Criteria Donors.
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Cantaluppi, V., Tamagnone, M., Dellepiane, S., Medica, D., Manzione, A. M., Messina, M., Figliolini, F., Ranghino, A., Biancone, L., Camussi, G., and Segoloni, G. P.
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- 2012
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13. Microvesicles Derived from Endothelial Progenitor Cells (EPCs) Protect from Antibody- and Complement-Mediated Endothelial Injury by Horizontal Transfer of Specific mRNAs and MicroRNAs: Potential Role in Graft Accomodation.
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Cantaluppi, V., Delena, M., Beltramo, S., Figliolini, F., Medica, D., Randone, O., Gallo, E., Tognarelli, G., Biancone, L., Segoloni, G. P., and Camussi, G.
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- 2012
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14. Isolation, Characterization and Pro-Angiogenic Activity of Microvesicles (MVs) Derived from Human Pancreatic Islets.
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Cantaluppi, V., Figliolini, F., De Lena, M., Beltramo, S., Medica, D., Biancone, L., Segoloni, G. P., and Camussi, G.
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- 2012
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15. Extracellular Vesicles Derived from Endothelial Progenitor Cells Protect Human Glomerular Endothelial Cells and Podocytes from Complement- and Cytokine-Mediated Injury.
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Medica D, Franzin R, Stasi A, Castellano G, Migliori M, Panichi V, Figliolini F, Gesualdo L, Camussi G, and Cantaluppi V
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- Apoptosis drug effects, Apoptosis genetics, Cell Movement drug effects, Cell Proliferation drug effects, Coculture Techniques, Endothelial Progenitor Cells cytology, Endothelial Progenitor Cells metabolism, Extracellular Vesicles chemistry, Gene Expression Regulation, Hepatocyte Growth Factor genetics, Hepatocyte Growth Factor metabolism, Humans, Intercellular Adhesion Molecule-1 genetics, Intercellular Adhesion Molecule-1 metabolism, L-Selectin genetics, L-Selectin metabolism, MicroRNAs genetics, MicroRNAs metabolism, Neovascularization, Physiologic drug effects, Neovascularization, Physiologic genetics, Paracrine Communication drug effects, Podocytes cytology, Podocytes metabolism, Primary Cell Culture, Reactive Oxygen Species antagonists & inhibitors, Reactive Oxygen Species metabolism, Vascular Cell Adhesion Molecule-1 genetics, Vascular Cell Adhesion Molecule-1 metabolism, Vascular Endothelial Growth Factor A genetics, Vascular Endothelial Growth Factor A metabolism, Complement C5a pharmacology, Endothelial Progenitor Cells drug effects, Extracellular Vesicles metabolism, Interleukin-6 pharmacology, Podocytes drug effects, Tumor Necrosis Factor-alpha pharmacology
- Abstract
Glomerulonephritis are renal inflammatory processes characterized by increased permeability of the Glomerular Filtration Barrier (GFB) with consequent hematuria and proteinuria. Glomerular endothelial cells (GEC) and podocytes are part of the GFB and contribute to the maintenance of its structural and functional integrity through the release of paracrine mediators. Activation of the complement cascade and pro-inflammatory cytokines (CK) such as Tumor Necrosis Factor α (TNF-α) and Interleukin-6 (IL-6) can alter GFB function, causing acute glomerular injury and progression toward chronic kidney disease. Endothelial Progenitor Cells (EPC) are bone-marrow-derived hematopoietic stem cells circulating in peripheral blood and able to induce angiogenesis and to repair injured endothelium by releasing paracrine mediators including Extracellular Vesicles (EVs), microparticles involved in intercellular communication by transferring proteins, lipids, and genetic material (mRNA, microRNA, lncRNA) to target cells. We have previously demonstrated that EPC-derived EVs activate an angiogenic program in quiescent endothelial cells and renoprotection in different experimental models. The aim of the present study was to evaluate in vitro the protective effect of EPC-derived EVs on GECs and podocytes cultured in detrimental conditions with CKs (TNF-α/IL-6) and the complement protein C5a. EVs were internalized in both GECs and podocytes mainly through a L-selectin-based mechanism. In GECs, EVs enhanced the formation of capillary-like structures and cell migration by modulating gene expression and inducing the release of growth factors such as VEGF-A and HGF. In the presence of CKs, and C5a, EPC-derived EVs protected GECs from apoptosis by decreasing oxidative stress and prevented leukocyte adhesion by inhibiting the expression of adhesion molecules (ICAM-1, VCAM-1, E-selectin). On podocytes, EVs inhibited apoptosis and prevented nephrin shedding induced by CKs and C5a. In a co-culture model of GECs/podocytes that mimicked GFB, EPC-derived EVs protected cell function and permeselectivity from inflammatory-mediated damage. Moreover, RNase pre-treatment of EVs abrogated their protective effects, suggesting the crucial role of RNA transfer from EVs to damaged glomerular cells. In conclusion, EPC-derived EVs preserved GFB integrity from complement- and cytokine-induced damage, suggesting their potential role as therapeutic agents for drug-resistant glomerulonephritis.
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- 2021
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16. Differential Therapeutic Effect of Extracellular Vesicles Derived by Bone Marrow and Adipose Mesenchymal Stem Cells on Wound Healing of Diabetic Ulcers and Correlation to Their Cargoes.
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Pomatto M, Gai C, Negro F, Cedrino M, Grange C, Ceccotti E, Togliatto G, Collino F, Tapparo M, Figliolini F, Lopatina T, Brizzi MF, and Camussi G
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- Adipose Tissue cytology, Animals, Bone Marrow Cells, Exosomes metabolism, Exosomes ultrastructure, Extracellular Vesicles ultrastructure, Flow Cytometry, Gene Expression Profiling, Immunohistochemistry, Mesenchymal Stem Cells cytology, Mice, Diabetes Complications therapy, Extracellular Vesicles metabolism, Mesenchymal Stem Cells metabolism, Ulcer etiology, Ulcer therapy, Wound Healing
- Abstract
Extracellular vesicles (EVs) derived from mesenchymal stem cells isolated from both bone marrow (BMSCs) and adipose tissue (ADSCs) show potential therapeutic effects. These vesicles often show a similar beneficial effect on tissue regeneration, but in some contexts, they exert different biological properties. To date, a comparison of their molecular cargo that could explain the different biological effect is not available. Here, we demonstrated that ADSC-EVs, and not BMSC-EVs, promote wound healing on a murine model of diabetic wounds. Besides a general similarity, the bioinformatic analysis of their protein and miRNA cargo highlighted important differences between these two types of EVs. Molecules present exclusively in ADSC-EVs were highly correlated to angiogenesis, whereas those expressed in BMSC-EVs were preferentially involved in cellular proliferation. Finally, in vitro analysis confirmed that both ADSC and BMSC-EVs exploited beneficial effect on cells involved in skin wound healing such as fibroblasts, keratinocytes and endothelial cells, but through different cellular processes. Consistent with the bioinformatic analyses, BMSC-EVs were shown to mainly promote proliferation, whereas ADSC-EVs demonstrated a major effect on angiogenesis. Taken together, these results provide deeper comparative information on the cargo of ADSC-EVs and BMSC-EVs and the impact on regenerative processes essential for diabetic wound healing.
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- 2021
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17. HLSC-Derived Extracellular Vesicles Attenuate Liver Fibrosis and Inflammation in a Murine Model of Non-alcoholic Steatohepatitis.
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Bruno S, Pasquino C, Herrera Sanchez MB, Tapparo M, Figliolini F, Grange C, Chiabotto G, Cedrino M, Deregibus MC, Tetta C, and Camussi G
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- Animals, Biomarkers, Disease Models, Animal, Gene Expression Profiling, Humans, Immunohistochemistry, Inflammation etiology, Inflammation metabolism, Inflammation pathology, Inflammation therapy, Liver Cirrhosis etiology, Liver Cirrhosis therapy, Mice, Non-alcoholic Fatty Liver Disease metabolism, Non-alcoholic Fatty Liver Disease pathology, Transcriptome, Extracellular Vesicles metabolism, Liver cytology, Liver metabolism, Liver Cirrhosis metabolism, Liver Cirrhosis pathology, Stem Cells cytology, Stem Cells metabolism
- Abstract
Extracellular vesicles (EVs) are membrane vesicles released virtually by all cell types. Several studies have shown that stem cell-derived EVs may mimic both in vitro and in vivo the biological effects of the cells. We recently demonstrated that non-alcoholic steatohepatitis (NASH) is inhibited by treatment with human liver stem cells (HLSCs). The aim of the present study was to evaluate whether EVs released by HLSCs influence the progression of NASH, induced by a diet deprived of methionine and choline, in immunocompromised mice. EV treatment was initiated after 2 weeks of diet with a biweekly administration of three different doses. Bio-distribution evaluated by optical imaging showed a preferential accumulation in normal and, in particular, in fibrotic liver. EV treatment significantly improved liver function and reduced signs of liver fibrosis and inflammation at both morphological and molecular levels. In particular, we observed that, out of 29 fibrosis-associated genes upregulated in NASH liver, 28 were significantly downregulated by EV treatment. In conclusion, HLSC-derived EVs display anti-fibrotic and anti-inflammatory effects in a model of chronic liver disease, leading to an improvement of liver function., (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2020
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18. miR-130a and Tgfβ Content in Extracellular Vesicles Derived from the Serum of Subjects at High Cardiovascular Risk Predicts their In-Vivo Angiogenic Potential.
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Cavallari C, Figliolini F, Tapparo M, Cedrino M, Trevisan A, Positello L, Rispoli P, Solini A, Migliaretti G, Camussi G, and Brizzi MF
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- 3' Untranslated Regions, Adult, Aged, Cardiovascular Diseases genetics, Cardiovascular Diseases metabolism, Case-Control Studies, Diabetes Mellitus, Type 2 genetics, Diabetes Mellitus, Type 2 metabolism, Extracellular Vesicles genetics, Extracellular Vesicles metabolism, Female, Humans, Male, Middle Aged, Cardiovascular Diseases blood, Diabetes Mellitus, Type 2 blood, Homeodomain Proteins genetics, MicroRNAs genetics, Transforming Growth Factor beta metabolism
- Abstract
Serum-derived extracellular vesicles (sEV) from healthy donors display in-vivo pro-angiogenic properties. To identify patients that may benefit from autologous sEV administration for pro-angiogenic purposes, sEV angiogenic capability has been evaluated in type 2 diabetic (T2DM) subjects (D), in obese individuals with (OD) and without (O) T2DM, and in subjects with ischemic disease (IC) (9 patients/group). sEV display different angiogenic properties in such cluster of individuals. miRNomic profile and TGFβ content in sEV were evaluated. We found that miR-130a and TGFβ content correlates with sEV in-vitro and in-vivo angiogenic properties, particularly in T2DM patients. Ingenuity Pathway Analysis (IPA) identified a number of genes as among the most significant miR-130a interactors. Gain-of-function experiments recognized homeoboxA5 (HOXA5) as a miR-130a specific target. Finally, ROC curve analyses revealed that sEV ineffectiveness could be predicted (Likelihood Ratio+ (LH+) = 3.3 IC 95% from 2.6 to 3.9) by comparing miR-130a and TGFβ content 'in Series'. We demonstrate that sEV from high cardiovascular risk patients have different angiogenic properties and that miR-130a and TGFβ sEV content predicts 'true ineffective sEVs'. These results provide the rationale for the use of these assays to identify patients that may benefit from autologous sEV administration to boost the angiogenetic process.
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- 2020
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19. Extracellular Vesicles From Adipose Stem Cells Prevent Muscle Damage and Inflammation in a Mouse Model of Hind Limb Ischemia: Role of Neuregulin-1.
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Figliolini F, Ranghino A, Grange C, Cedrino M, Tapparo M, Cavallari C, Rossi A, Togliatto G, Femminò S, Gugliuzza MV, Camussi G, and Brizzi MF
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- Adipocytes metabolism, Animals, Blotting, Western, Cell Differentiation, Cells, Cultured, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Extracellular Vesicles ultrastructure, Ischemia metabolism, Male, Mice, Mice, Inbred C57BL, Microscopy, Electron, Transmission, Muscle, Skeletal metabolism, Proteomics, Stem Cells metabolism, Adipocytes cytology, Extracellular Vesicles metabolism, Hindlimb blood supply, Ischemia pathology, Muscle, Skeletal ultrastructure, Neuregulin-1 metabolism, Stem Cells ultrastructure
- Abstract
Objectives: Critical hindlimb ischemia is a severe consequence of peripheral artery disease. Surgical treatment does not prevent skeletal muscle impairment or improve long-term patient outcomes. The present study investigates the protective/regenerative potential and the mechanism of action of adipose stem cell-derived extracellular vesicles (ASC-EVs) in a mouse model of hindlimb ischemia. Approach and Results: We demonstrated that ASC-EVs exert a protective effect on muscle damage by acting both on tissue microvessels and muscle cells. The genes involved in muscle regeneration were up-regulated in the ischemic muscles of ASC-EV-treated animals. MyoD expression has also been confirmed in satellite cells. This was followed by a reduction in muscle function impairment in vivo. ASC-EVs drive myoblast proliferation and differentiation in the in vitro ischemia/reoxygenation model. Moreover, ASC-EVs have shown an anti-apoptotic effect both in vitro and in vivo. Transcriptomic analyses have revealed that ASC-EVs carry a variety of pro-angiogenic mRNAs, while proteomic analyses have demonstrated an enrichment of NRG1 (neuregulin 1). A NRG1 blocking antibody used in vivo demonstrated that NRG1 is relevant to ASC-EV-induced muscle protection, vascular growth, and recruitment of inflammatory cells. Finally, bioinformatic analyses on 18 molecules that were commonly detected in ASC-EVs, including mRNAs and proteins, confirmed the enrichment of pathways involved in vascular growth and muscle regeneration/protection., Conclusions: This study demonstrates that ASC-EVs display pro-angiogenic and skeletal muscle protective properties that are associated with their NRG1/mRNA cargo. We, therefore, propose that ASC-EVs are a useful tool for therapeutic angiogenesis and muscle protection.
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- 2020
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20. Perfluorocarbon solutions limit tubular epithelial cell injury and promote CD133+ kidney progenitor differentiation: potential use in renal assist devices for sepsis-associated acute kidney injury and multiple organ failure.
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Cantaluppi V, Medica D, Quercia AD, Dellepiane S, Figliolini F, Virzì GM, Brocca A, Quaglia M, Marengo M, Olivieri C, Senzolo M, Garzotto F, Della Corte F, Castellano G, Gesualdo L, Camussi G, and Ronco C
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- Acute Kidney Injury diagnosis, Acute Kidney Injury etiology, Aged, Aged, 80 and over, Animals, Cell Differentiation drug effects, Cell Proliferation drug effects, Cells, Cultured, Female, Humans, Kidney Tubules drug effects, Kidney Tubules metabolism, Kidney Tubules pathology, Male, Middle Aged, Multiple Organ Failure diagnosis, Multiple Organ Failure etiology, Sepsis pathology, Sepsis therapy, Stem Cells drug effects, Stem Cells metabolism, AC133 Antigen metabolism, Acute Kidney Injury therapy, Apoptosis drug effects, Fluorocarbons pharmacology, Multiple Organ Failure therapy, Sepsis complications, Stem Cells pathology
- Abstract
Background: The renal assist device (RAD) is a blood purification system containing viable renal tubular epithelial cells (TECs) that has been proposed for the treatment of acute kidney injury (AKI) and multiple organ failure. Perfluorocarbons (PFCs) are oxygen carriers used for organ preservation in transplantation. The aim of this study was to investigate the effect of PFCs on hypoxia- and sepsis-induced TEC injury and on renal CD133+ progenitor differentiation in a microenvironment similar to the RAD., Methods: TECs were seeded in a polysulphone hollow fibre under hypoxia or cultured with plasma from 10 patients with sepsis-associated AKI in the presence or absence of PFCs and were tested for cytotoxicity (XTT assay), apoptosis (terminal deoxynucleotidyl transferase dUTP nick end labeling assay, caspases, enzyme-linked immunosorbent assay, Fas/Fas Ligand pathway activation), mitochondrial activity, cell polarity [transepithelial electrical resistance (TEER)] and adenosine triphosphate production. The effect of PFCs on proliferation and differentiation of human CD133+ progenitors was also studied., Results: In the presence of PFCs, TECs seeded into the polysulphone hollow fibre showed increased viability and expression of insulin-like growth factor 1, hepatocyte growth factor and macrophage-stimulating protein. Plasma from septic patients induced TEC apoptosis, disruption of oxidative metabolism, alteration of cell polarity and albumin uptake, down-regulation of the tight junction protein ZO-1 and the endocytic receptor megalin on the TEC surface. These detrimental effects were significantly reduced by PFCs. Moreover, PFCs induced CD133+ renal progenitor cell proliferation and differentiation towards an epithelial/tubular-like phenotype., Conclusions: PFCs improved the viability and metabolic function of TECs seeded within a polysulphone hollow fibre and subjected to plasma from septic AKI patients. Additionally, PFCs promoted differentiation towards a tubular/epithelial phenotype of CD133+ renal progenitor cells.
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- 2018
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21. Serum-derived extracellular vesicles (EVs) impact on vascular remodeling and prevent muscle damage in acute hind limb ischemia.
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Cavallari C, Ranghino A, Tapparo M, Cedrino M, Figliolini F, Grange C, Giannachi V, Garneri P, Deregibus MC, Collino F, Rispoli P, Camussi G, and Brizzi MF
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- Animals, Biomarkers, Cell Proliferation, Disease Models, Animal, Endothelial Cells metabolism, Extracellular Vesicles ultrastructure, Gene Expression Profiling, Gene Expression Regulation, Hindlimb, Immunohistochemistry, Mice, Muscle, Skeletal blood supply, Neovascularization, Physiologic, Promoter Regions, Genetic, Proteomics methods, Reperfusion Injury metabolism, Reperfusion Injury pathology, Transcriptome, Extracellular Vesicles metabolism, Ischemia blood, Ischemia pathology, Muscle, Skeletal metabolism, Muscle, Skeletal pathology, Vascular Remodeling
- Abstract
Serum is an abundant and accessible source of circulating extracellular vesicles (EVs). Serum-EV (sEV) pro-angiogenic capability and mechanisms are herein analyzed using an in vitro assay which predicts sEV angiogenic potential in vivo. Effective sEVs (e-sEVs) also improved vascular remodeling and prevented muscle damage in a mouse model of acute hind limb ischemia. e-sEV angiogenic proteomic and transcriptomic analyses show a positive correlation with matrix-metalloproteinase activation and extracellular matrix organization, cytokine and chemokine signaling pathways, Insulin-like Growth Factor and platelet pathways, and Vascular Endothelial Growth Factor signaling. A discrete gene signature, which highlights differences in e-sEV and ineffective-EV biological activity, was identified using gene ontology (GO) functional analysis. An enrichment of genes associated with the Transforming Growth Factor beta 1 (TGFβ1) signaling cascade is associated with e-sEV administration but not with ineffective-EVs. Chromatin immunoprecipitation analysis on the inhibitor of DNA binding I (ID1) promoter region, and the knock-down of small mother against decapentaplegic (SMAD)1-5 proteins confirmed GO functional analyses. This study demonstrates sEV pro-angiogenic activity, validates a simple, sEV pro-angiogenic assay which predicts their biological activity in vivo, and identifies the TGFβ1 cascade as a relevant mediator. We propose serum as a readily available source of EVs for therapeutic purposes.
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- 2017
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22. Charge-based precipitation of extracellular vesicles.
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Deregibus MC, Figliolini F, D'Antico S, Manzini PM, Pasquino C, De Lena M, Tetta C, Brizzi MF, and Camussi G
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- Adult, Apolipoproteins metabolism, Humans, Liver cytology, Nanoparticles chemistry, RNA metabolism, Saliva chemistry, Serum metabolism, Stem Cells metabolism, Ultracentrifugation, Chemical Precipitation, Extracellular Vesicles chemistry, Static Electricity
- Abstract
Vesicular-mediated communication between cells appears critical in many biological processes. Extracellular vesicles (EVs) released from healthy and diseased cells are involved in a network of exchange of biologically active molecules. Since EVs present in biological fluids carry the signature of the cell of origin, they are potential biomarkers for ongoing physiological or pathological processes. Despite the knowledge on EV biology accrued in recent years, techniques of EV purification remain a challenge and all the described methods have some advantages and disadvantages. In the present study, we described a method based on charge precipitation of EVs from biological fluids and from cell supernatants in comparison with the differential ultracentrifugation, which is considered the gold standard for EV purification. The analysis of ζ‑potential revealed that EVs have a negative charge that allows the interaction with a positively charged molecule, such as protamine. Protamine was shown to induce EV precipitation from serum and saliva and from cell culture media without the need for ultracentrifugation. EV resuspension was facilitated when protamine (P) precipitation was performed in the presence of PEG 35,000 Da (P/PEG precipitation). The recovery of precipitated EVs evaluated by NanoSight analysis was more efficient than that obtained by ultracentrifugation. By electron microscopy the size of EVs was similar after both methods were used, and the expression of CD63, CD9 and CD81 exosomal markers in the P/PEG‑precipitated EVs indicated an enrichment in exosomes. The RNA recovery of P/PEG‑precipitated EVs was similar to that of EVs isolated by ultracentrifugation. In addition, P/PEG‑precipitated EVs retained the biological activity in vitro as observed by the induction of wound closure by keratinocytes and of proliferation of tubular epithelial cells. In conclusion, charge-based precipitation of EVs has the merit of simplicity and avoids the requirement of expensive equipments and may be used for the efficient isolation of EVs from small biological samples.
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- 2016
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23. Neutrophil Gelatinase Associated Lipocalin Is an Early and Accurate Biomarker of Graft Function and Tissue Regeneration in Kidney Transplantation from Extended Criteria Donors.
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Cantaluppi V, Dellepiane S, Tamagnone M, Medica D, Figliolini F, Messina M, Manzione AM, Gai M, Tognarelli G, Ranghino A, Dolla C, Ferrario S, Tetta C, Segoloni GP, Camussi G, and Biancone L
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- Acute-Phase Proteins genetics, Aged, Apoptosis drug effects, Biomarkers blood, Cell Hypoxia, Cells, Cultured, Cohort Studies, Delayed Graft Function blood, Delayed Graft Function etiology, Donor Selection, Female, Gene Expression, Humans, Immunosuppressive Agents adverse effects, Kidney drug effects, Kidney physiopathology, Kidney Tubules drug effects, Kidney Tubules pathology, Kidney Tubules physiopathology, Lipocalin-2, Lipocalins genetics, Male, Middle Aged, Prospective Studies, Proto-Oncogene Proteins genetics, Regeneration, Tacrolimus adverse effects, Kidney Transplantation adverse effects, Lipocalins blood, Proto-Oncogene Proteins blood, Tissue Donors
- Abstract
Background: Delayed graft function (DGF) is an early complication of kidney transplantation (KT) associated with increased risk of early loss of graft function. DGF increases using kidneys from extended criteria donors (ECD). NGAL is a 25KDa protein proposed as biomarker of acute kidney injury. The aim of this study was to investigate the role of NGAL as an early and accurate indicator of DGF and Tacrolimus (Tac) toxicity and as a mediator of tissue regeneration in KT from ECD., Methods: We evaluated plasma levels of NGAL in 50 KT patients from ECD in the first 4 days after surgery or after Tac introduction., Results: Plasma levels of NGAL at day 1 were significantly higher in DGF group. In the non DGF group, NGAL discriminated between slow or immediate graft function and decreased more rapidly than serum creatinine. NGAL increased after Tac introduction, suggesting a role as marker of drug toxicity. In vitro, hypoxia and Tac induced NGAL release from tubular epithelial cells (TEC) favoring an autocrine loop that sustains proliferation and inhibits apoptosis (decrease of caspases and Bax/Bcl-2 ratio)., Conclusions: NGAL is an early and accurate biomarker of graft function in KT from ECD favoring TEC regeneration after ischemic and nephrotoxic injury.
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- 2015
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24. Recellularization of rat liver scaffolds by human liver stem cells.
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Navarro-Tableros V, Herrera Sanchez MB, Figliolini F, Romagnoli R, Tetta C, and Camussi G
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- Albumins biosynthesis, Albumins genetics, Animals, Apoptosis, Cell Adhesion, Cell Differentiation, Cell Division, Culture Media, Conditioned chemistry, Cytochrome P-450 Enzyme System biosynthesis, Cytochrome P-450 Enzyme System genetics, Fetal Proteins biosynthesis, Fetal Proteins genetics, Gene Expression Profiling, Humans, Intermediate Filament Proteins biosynthesis, Intermediate Filament Proteins genetics, L-Lactate Dehydrogenase biosynthesis, L-Lactate Dehydrogenase genetics, Male, Nitrogen analysis, Platelet Endothelial Cell Adhesion Molecule-1 biosynthesis, Platelet Endothelial Cell Adhesion Molecule-1 genetics, Rats, Rats, Wistar, Urea metabolism, Adult Stem Cells cytology, Extracellular Matrix, Hepatocytes cytology, Liver ultrastructure, Tissue Scaffolds
- Abstract
In the present study, rat liver acellular scaffolds were used as biological support to guide the differentiation of human liver stem-like cells (HLSC) to hepatocytes. Once recellularized, the scaffolds were maintained for 21 days in different culture conditions to evaluate hepatocyte differentiation. HLSC lost the embryonic markers (alpha-fetoprotein, nestin, nanog, sox2, Musashi1, Oct 3/4, and pax2), increased the expression of albumin, and acquired the expression of lactate dehydrogenase and three subtypes of cytochrome P450. The presence of urea nitrogen in the culture medium confirmed their metabolic activity. In addition, cells attached to tubular remnant matrix structures expressed cytokeratin 19, CD31, and vimentin. The rat extracellular matrix (ECM) provides not only a favorable environment for differentiation of HLSC in functional hepatocytes (hepatocyte like) but also promoted the generation of some epithelial-like and endothelial-like cells. When fibroblast growth factor-epidermal growth factor or HLSC-derived conditioned medium was added to the perfusate, an improvement of survival rate was observed. The conditioned medium from HLSC potentiated also the metabolic activity of hepatocyte-like cells repopulating the acellular liver. In conclusion, HLSC have the potential, in association with the natural ECM, to generate in vitro a functional "humanized liver-like tissue."
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- 2015
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25. Endothelial progenitor cell-derived extracellular vesicles protect from complement-mediated mesangial injury in experimental anti-Thy1.1 glomerulonephritis.
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Cantaluppi V, Medica D, Mannari C, Stiaccini G, Figliolini F, Dellepiane S, Quercia AD, Migliori M, Panichi V, Giovannini L, Bruno S, Tetta C, Biancone L, and Camussi G
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- Animals, Apoptosis, Cells, Cultured, Female, Fluorescent Antibody Technique, Glomerular Mesangium injuries, Glomerular Mesangium pathology, Glomerulonephritis pathology, Humans, RNA, Messenger genetics, Rats, Rats, Wistar, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Complement Membrane Attack Complex immunology, Endothelial Progenitor Cells immunology, Extracellular Vesicles immunology, Glomerular Mesangium immunology, Glomerulonephritis immunology, Isoantibodies immunology, Proteinuria immunology
- Abstract
Background: Endothelial progenitor cells (EPCs) are known to induce tissue repair by paracrine mechanisms including the release of growth factors and extracellular vesicles (EVs), nanoparticles able to carry proteins and genetic information to target cells. The aim of this study was to evaluate whether EVs derived from EPCs may protect from complement-mediated mesangial injury in experimental anti-Thy1.1 glomerulonephritis., Methods: EVs were isolated by serial ultracentrifugation from supernatants of cultured human EPCs and characterized for their protein and RNA content. In vivo, EVs were injected i.v. in the experimental rat model of mesangiolytic anti-Thy1.1 glomerulonephritis evaluating renal function, proteinuria, complement activity and histological lesions. In vitro, the biological effects of EPC-derived EVs were studied in cultured rat mesangial cells incubated with anti-Thy1.1 antibody and rat or human serum as complement source., Results: After i.v. injection in Thy1.1-treated rats, EVs localized within injured glomeruli and inhibited mesangial cell activation, leucocyte infiltration and apoptosis, decreased proteinuria, increased serum complement haemolytic activity (CH50) and ameliorated renal function. EV treatment decreased intraglomerular deposition of the membrane attack complex (MAC or C5b-9) and expression of smooth muscle cell actin and preserved the endothelial antigen RECA-1 and the podocyte marker synaptopodin. The protective effect of EVs was significantly reduced by pre-treatment with a high dose of RNase (1 U/mL), suggesting a key role for EV-carried RNAs in these mechanisms. Indeed, EPC-derived EVs contained different mRNAs coding for several anti-apoptotic molecules and for the complement inhibitors Factor H, CD55 and CD59 and the related proteins. The in vitro experiments aimed to investigate the mechanisms of EV protection indicated that EVs transferred to mesangial cell mRNAs coding for Factor H, CD55 and CD59 and inhibited anti-Thy1.1 antibody/complement-induced apoptosis and C5b-9/C3 mesangial cell deposition., Conclusions: EVs derived from EPCs exert a protective effect in Thy1.1 glomerulonephritis by inhibition of antibody- and complement-mediated injury of mesangial cells., (© The Author 2014. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.)
- Published
- 2015
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26. Isolation, characterization and potential role in beta cell-endothelium cross-talk of extracellular vesicles released from human pancreatic islets.
- Author
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Figliolini F, Cantaluppi V, De Lena M, Beltramo S, Romagnoli R, Salizzoni M, Melzi R, Nano R, Piemonti L, Tetta C, Biancone L, and Camussi G
- Subjects
- Blotting, Western, Endothelial Cells cytology, Flow Cytometry, Humans, MicroRNAs metabolism, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Angiogenesis Inducing Agents metabolism, Cell Communication physiology, Endothelial Cells physiology, Insulin-Secreting Cells physiology, Receptor Cross-Talk physiology, Transport Vesicles metabolism
- Abstract
The cross-talk between beta cells and endothelium plays a key role in islet physiopathology and in the revascularization process after islet transplantation. However, the molecular mechanisms involved in this cross-talk are not fully elucidated. Extracellular vesicles (EVs) are secreted membrane nanoparticles involved in inter-cellular communication through the transfer of proteins and nucleic acids. The aims of this study were: 1) isolation and characterization of EVs from human islets; 2) evaluation of the pro-angiogenic effect of islet-derived EVs on human islet endothelial cells (IECs). EVs were isolated by ultracentrifugation from conditioned medium of human islets and characterized by nanotrack analysis (Nanosight), FACS, western blot, bioanalyzer, mRNA/microRNA RT-PCR array. On IECs, we evaluated EV-induced insulin mRNA transfer, proliferation, resistance to apoptosis, in vitro angiogenesis, migration, gene and protein profiling. EVs sized 236±54 nm, expressed different surface molecules and islet-specific proteins (insulin, C-peptide, GLP1R) and carried several mRNAs (VEGFa, eNOS) and microRNAs (miR-27b, miR-126, miR-130 and miR-296) involved in beta cell function, insulin secretion and angiogenesis. Purified EVs were internalized into IECs inducing insulin mRNA expression, protection from apoptosis and enhancement of angiogenesis. Human islets release biologically active EVs able to shuttle specific mRNAs and microRNAs (miRNAs) into target endothelial cells. These results suggest a putative role for islet-derived EVs in beta cell-endothelium cross-talk and in the neoangiogenesis process which is critical for engraftment of transplanted islets.
- Published
- 2014
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27. Microvesicles derived from endothelial progenitor cells protect the kidney from ischemia-reperfusion injury by microRNA-dependent reprogramming of resident renal cells.
- Author
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Cantaluppi V, Gatti S, Medica D, Figliolini F, Bruno S, Deregibus MC, Sordi A, Biancone L, Tetta C, and Camussi G
- Subjects
- Acute Kidney Injury genetics, Acute Kidney Injury metabolism, Acute Kidney Injury pathology, Animals, Apoptosis, Capillaries metabolism, Capillaries pathology, Cell Hypoxia, Cell Proliferation, Cell-Derived Microparticles metabolism, Cell-Derived Microparticles pathology, Cells, Cultured, Chemotaxis, Leukocyte, Disease Models, Animal, Endothelial Cells metabolism, Endothelial Cells pathology, Epithelial Cells metabolism, Epithelial Cells pathology, Fibrosis, Gene Expression Regulation, Kidney blood supply, Kidney pathology, Kidney Tubules metabolism, Kidney Tubules pathology, Male, Oligonucleotides metabolism, RNA Interference, Rats, Rats, Wistar, Regeneration, Reperfusion Injury genetics, Reperfusion Injury metabolism, Reperfusion Injury pathology, Ribonuclease III genetics, Ribonuclease III metabolism, Time Factors, Transfection, Acute Kidney Injury prevention & control, Cell-Derived Microparticles transplantation, Endothelial Cells transplantation, Kidney metabolism, MicroRNAs metabolism, Reperfusion Injury prevention & control, Stem Cell Transplantation, Stem Cells metabolism, Stem Cells pathology
- Abstract
Endothelial progenitor cells are known to reverse acute kidney injury by paracrine mechanisms. We previously found that microvesicles released from these progenitor cells activate an angiogenic program in endothelial cells by horizontal mRNA transfer. Here, we tested whether these microvesicles prevent acute kidney injury in a rat model of ischemia-reperfusion injury. The RNA content of microvesicles was enriched in microRNAs (miRNAs) that modulate proliferation, angiogenesis, and apoptosis. After intravenous injection following ischemia-reperfusion, the microvesicles were localized within peritubular capillaries and tubular cells. This conferred functional and morphologic protection from acute kidney injury by enhanced tubular cell proliferation, reduced apoptosis, and leukocyte infiltration. Microvesicles also protected against progression of chronic kidney damage by inhibiting capillary rarefaction, glomerulosclerosis, and tubulointerstitial fibrosis. The renoprotective effect of microvesicles was lost after treatment with RNase, nonspecific miRNA depletion of microvesicles by Dicer knock-down in the progenitor cells, or depletion of pro-angiogenic miR-126 and miR-296 by transfection with specific miR-antagomirs. Thus, microvesicles derived from endothelial progenitor cells protect the kidney from ischemic acute injury by delivering their RNA content, the miRNA cargo of which contributes to reprogramming hypoxic resident renal cells to a regenerative program.
- Published
- 2012
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28. Microvesicles derived from endothelial progenitor cells enhance neoangiogenesis of human pancreatic islets.
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Cantaluppi V, Biancone L, Figliolini F, Beltramo S, Medica D, Deregibus MC, Galimi F, Romagnoli R, Salizzoni M, Tetta C, Segoloni GP, and Camussi G
- Subjects
- Angiogenesis Inhibitors pharmacology, Animals, Apoptosis, CD40 Antigens metabolism, Cell Proliferation, DEAD-box RNA Helicases antagonists & inhibitors, DEAD-box RNA Helicases genetics, DEAD-box RNA Helicases metabolism, Humans, Insulin metabolism, Insulin Secretion, Insulin-Secreting Cells metabolism, Islets of Langerhans metabolism, Islets of Langerhans Transplantation, L-Selectin metabolism, Leukocytes cytology, Leukocytes immunology, Mice, Mice, SCID, MicroRNAs metabolism, Nitric Oxide Synthase Type III metabolism, Phosphatidylinositol 3-Kinases metabolism, Proto-Oncogene Proteins c-akt metabolism, Ribonuclease III antagonists & inhibitors, Ribonuclease III genetics, Ribonuclease III metabolism, Ribonucleases metabolism, Signal Transduction, Sirolimus pharmacology, Transplantation, Heterologous, Endothelial Cells cytology, Islets of Langerhans blood supply, Neovascularization, Physiologic, Stem Cells cytology
- Abstract
The efficacy of islet transplantation is limited by poor graft vascularization. We herein demonstrated that microvesicles (MVs) released from endothelial progenitor cells (EPCs) enhanced human islet vascularization. After incorporation into islet endothelium and β-cells, EPC-derived MVs favored insulin secretion, survival, and revascularization of islets transplanted in SCID mice. MVs induced in vitro islet endothelial cell proliferation, migration, resistance to apoptosis, and organization in vessel-like structures. Moreover, MVs partially overcame the antiangiogenic effect of rapamycin and inhibited endothelial-leukocyte interaction via L-selectin and CD40. MVs were previously shown to contain defined patterns of mRNAs. Here we demonstrated that MVs carried the proangiogenic miR-126 and miR-296 microRNAs (miRNAs). MVs pretreated with RNase or derived from Dicer knocked-down EPCs showed a reduced angiogenic effect. In addition, MVs overcame the antiangiogenic effect of the specific antagomiRs of miR-126 and miR-296, suggesting a relevant contribution of miRNAs delivered by MVs to islet endothelium. Microarray analysis of MV-stimulated islet endothelium indicated the upregulation of mRNAs coding for factors involved in endothelial proliferation, differentiation, and angiogenesis. In addition, MVs induced the activation of the PI3K-Akt and eNOS signaling pathways in islet endothelium. These results suggest that MVs activate an angiogenic program in islet endothelium that may sustain revascularization and β-cell function.
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- 2012
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29. Protective effect of resin adsorption on septic plasma-induced tubular injury.
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Cantaluppi V, Weber V, Lauritano C, Figliolini F, Beltramo S, Biancone L, De Cal M, Cruz D, Ronco C, Segoloni GP, Tetta C, and Camussi G
- Subjects
- Adsorption, Aged, Cells, Cultured, Cytokines blood, Female, Humans, In Vitro Techniques, Inflammation Mediators blood, Kidney Diseases etiology, Kidney Diseases pathology, Male, Middle Aged, Sepsis complications, Cytokines isolation & purification, Inflammation Mediators isolation & purification, Kidney Diseases prevention & control, Kidney Tubules, Proximal pathology, Polymers pharmacology, Sepsis blood
- Abstract
Introduction: A pro-apoptotic effect of circulating mediators on renal tubular epithelial cells has been involved in the pathogenesis of sepsis-associated acute kidney injury (AKI). Adsorption techniques have been showed to efficiently remove inflammatory cytokines from plasma. The aim of this study was to evaluate the efficiency of the hydrophobic resin Amberchrom CG161 M to adsorb from septic plasma soluble mediators involved in tubular injury., Methods: We enrolled in the study 10 critically ill patients with sepsis-associated AKI and we evaluated the effects of their plasma on granulocyte adhesion, apoptosis and functional alterations of cultured human kidney tubular epithelial cells. We established an in vitro model of plasma adsorption and we studied the protective effect of unselective removal of soluble mediators by the Amberchrom CG161 M resin on septic plasma-induced tubular cell injury., Results: Plasma from septic patients induced granulocyte adhesion, apoptosis and altered polarity in tubular cells. Plasma adsorption significantly decreased these effects and abated the concentrations of several soluble mediators. The inhibition of granulocyte adhesion to tubular cells was associated with the down-regulation of ICAM-1 and CD40. Resin adsorption inhibited tubular cell apoptosis induced by septic plasma by down-regulating the activation of caspase-3, 8, 9 and of Fas/death receptor-mediated signalling pathways. The alteration of cell polarity, morphogenesis, protein reabsorption and the down-regulation of the tight junction molecule ZO-1, of the sodium transporter NHE3, of the glucose transporter GLUT-2 and of the endocytic receptor megalin all induced by septic plasma were significantly reduced by resin adsorption., Conclusions: Septic plasma induced a direct injury of tubular cells by favouring granulocyte adhesion, by inducing cell apoptosis and by altering cell polarity and function. All these biological effects are related to the presence of circulating inflammatory mediators that can be efficiently removed by resin adsorption with a consequent limitation of tubular cell injury.
- Published
- 2010
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30. Macrophage stimulating protein may promote tubular regeneration after acute injury.
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Cantaluppi V, Biancone L, Romanazzi GM, Figliolini F, Beltramo S, Galimi F, Camboni MG, Deriu E, Conaldi P, Bottelli A, Orlandi V, Herrera MB, Pacitti A, Segoloni GP, and Camussi G
- Subjects
- Aged, Animals, Case-Control Studies, Cell Culture Techniques, Cell Survival, Critical Illness, Humans, Mice, Mice, Inbred C57BL, Middle Aged, Acute Kidney Injury blood, Hepatocyte Growth Factor blood, Kidney Transplantation, Kidney Tubules physiology, Proto-Oncogene Proteins blood, Receptor Protein-Tyrosine Kinases blood, Regeneration physiology
- Abstract
Macrophage-stimulating protein (MSP) exerts proliferative and antiapoptotic effects, suggesting that it may play a role in tubular regeneration after acute kidney injury. In this study, elevated plasma levels of MSP were found both in critically ill patients with acute renal failure and in recipients of renal allografts during the first week after transplantation. In addition, MSP and its receptor, RON, were markedly upregulated in the regenerative phase after glycerol-induced tubular injury in mice. In vitro, MSP stimulated tubular epithelial cell proliferation and conferred resistance to cisplatin-induced apoptosis by inhibiting caspase activation and modulating Fas, mitochondrial proteins, Akt, and extracellular signal-regulated kinase. MSP also enhanced migration, scattering, branching morphogenesis, tubulogenesis, and mesenchymal de-differentiation of surviving tubular cells. In addition, MSP induced an embryonic phenotype characterized by Pax-2 expression. In conclusion, MSP is upregulated during the regeneration of injured tubular cells, and it exerts multiple biologic effects that may aid recovery from acute kidney injury.
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- 2008
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31. Magnetic resonance imaging of gadolinium-labeled pancreatic islets for experimental transplantation.
- Author
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Biancone L, Crich SG, Cantaluppi V, Romanazzi GM, Russo S, Scalabrino E, Esposito G, Figliolini F, Beltramo S, Perin PC, Segoloni GP, Aime S, and Camussi G
- Subjects
- Animals, Contrast Media pharmacology, Gadolinium, Gene Expression Profiling, Gene Expression Regulation drug effects, Heterocyclic Compounds, Humans, Islets of Langerhans drug effects, Islets of Langerhans metabolism, Kidney, Liver, Mass Spectrometry, Mice, Mice, SCID, Oligonucleotide Array Sequence Analysis, Organometallic Compounds pharmacology, Portal Vein, Transplantation, Heterologous, Transplantation, Isogeneic, Contrast Media analysis, Islets of Langerhans cytology, Islets of Langerhans Transplantation, Magnetic Resonance Imaging, Organometallic Compounds analysis, Transplantation, Heterotopic
- Abstract
New imaging techniques that couple anatomical resolution to sensitivity may greatly contribute to improving islet transplantation. In the present work, a report is given of the direct detection of islets by magnetic resonance imaging (MRI) after ex vivo cell labeling with the MRI T(1) contrast agent GdHPDO3A. Experiments on mouse and human islets demonstrated well-tolerated uptake of GdHPDO3A, based on morphology, viability, glucose-dependent insulin response and apoptosis/toxicity gene array profile. GdHPDO3A loading was sufficient for in vitro MRI cell detection. In vivo isotransplanted mouse islets into the kidney capsule and xenotransplanted human islets within the mouse liver were detected. Imaging specificity was supported by the absence of signal in unlabeled islet transplants, its persistence upon using fat-suppression MRI protocols and the colocalization with the transplanted islets. In conclusion, direct islet imaging with high spatial and contrast resolution after labeling with GdHPDO3A is demonstrated, allowing visualization of kidney subcapsular mouse islet grafts and intrahepatic human islet xenografts.
- Published
- 2007
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32. Platelet-activating factor synthesis and response on pancreatic islet endothelial cells: relevance for islet transplantation.
- Author
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Biancone L, Cantaluppi V, Romanazzi GM, Russo S, Figliolini F, Beltramo S, Scalabrino E, Deregibus MC, Romagnoli R, Franchello A, Salizzoni M, Perin PC, Ricordi C, Segoloni GP, and Camussi G
- Subjects
- Animals, Antigens, Polyomavirus Transforming genetics, Cell Line, Cell Movement, Cells, Cultured, Humans, Islets of Langerhans blood supply, Mice, Oligonucleotide Array Sequence Analysis, Platelet Activating Factor genetics, Transfection, Endothelium, Vascular physiology, Islets of Langerhans physiology, Islets of Langerhans Transplantation physiology, Platelet Activating Factor biosynthesis
- Abstract
Background: Recent data suggest that donor intraislet endothelial cells may survive islet transplantation and participate to the events that influence islet engraftment. However, the mechanisms that regulate islet endothelial behavior in this setting are poorly known., Methods: We obtained immortalized human (hIECs) and mouse (mIECs) islet endothelial cells by transfection with SV40-T-large antigen and studied the synthesis and response to Platelet-activating factor (PAF), a multipotent phospholipid that acts as endothelial mediator of both inflammation and angiogenesis., Results: HIECs showed typical endothelial markers such as expression of vWF, CD31, and CD105, uptake of acetylated-LDL and binding to ULE-A lectin. Moreover, they expressed nestin, the PAF-receptor and possess surface fenestrations and in vitro angiogenic ability of forming tubular structures on Matrigel. Likewise, mIECs showed expression of vWF, CD31, nestin, PAF-receptor and CD105, and uptake of acetylated-LDL. HIECs and mIECs rapidly produced PAF under stimulation with thrombin in a dose-dependent way. Exogenous PAF or thrombin-induced PAF synthesis increased leukocyte adhesion to hIECS and mIECs and cell motility of both endothelial cell lines. Moreover, PAF or thrombin-induced PAF synthesis accelerated in vitro formation of vessel-like tubular structures when hIECs are seeded on Matrigel. Notably, gene-microarray analysis detected up-regulation of beta3 integrin gene on hIECs stimulated with PAF, that was confirmed at the protein level., Conclusions: Based on the novel development of immortalized islet endothelium, these results suggest that PAF may have a dual role that links inflammation to angiogenesis in the early events of islet transplantation.
- Published
- 2006
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33. A new method of colorectal anastomosis in abdominoperineal resection.
- Author
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CUTAIT DE and FIGLIOLINI FJ
- Subjects
- Humans, Anastomosis, Surgical, Colon surgery, Colon, Sigmoid, Digestive System Surgical Procedures, Rectum surgery
- Published
- 1961
- Full Text
- View/download PDF
34. Rectosigmoidal hemangioma: report of two cases.
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FIGLIOLINI FJ, CUTAIT DE, de OLIVEIRA M, and da BASTOS ES
- Subjects
- Humans, Colon, Sigmoid, Hemangioma, Intestine, Large, Medical Records, Rectal Neoplasms, Rectum, Sigmoid Neoplasms
- Published
- 1961
- Full Text
- View/download PDF
35. Giant follicular lymphoblastoma of the rectum (Brill-Symmers disease).
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Figliolini FJ, Cutait DE, de Oliveira MR, and Bastos Eda S
- Subjects
- Child, Colon diagnostic imaging, Contrast Media, Diagnosis, Differential, Female, Humans, Lymphoma, Follicular etiology, Polyps surgery, Prognosis, Radiography, Rectal Neoplasms surgery, Lymphoma, Follicular diagnosis
- Published
- 1967
- Full Text
- View/download PDF
36. SUBMUCOUS SUBSEROUS LIPOMA OF THE COLON: REPORT OF A CASE.
- Author
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FIGLIOLINI FJ, CUTAIT DE, DE OLIVEIRA MR, and BASTOSEDA S
- Subjects
- Colonic Neoplasms, Lipoma, Pathology, Radiography, Surgical Procedures, Operative
- Published
- 1964
- Full Text
- View/download PDF
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