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Alessandra Buja,1 Stefania Elvini,2 Riccardo Caberlotto,3 Carlo Pinato,1 Simona Fortunata Mafrici,3 Giulia Grotto,3 Enrica Bicciato,3 Tatjana Baldovin,1 Giulia Zumerle,2 Pietro Gallina,4 Vincenzo Baldo1 1Department of Cardiologic, Vascular, and Thoracic Sciences, and Public Health, University of Padua, Padova, Italy; 2Controllo di Gestione, AULSS 6 Euganea, Regione Veneto, Padova, Italy; 3School of Specialization in Hygiene, Preventive Medicine and Public Health, University of Padua, Padova, Italy; 4Direzione Sanitaria, AULSS 6 Euganea, Regione Veneto, Padova, ItalyCorrespondence: Giulia GrottoHygiene and Public Health Unit, Department of Cardiologic, Vascular, and Thoracic Sciences and Public Health, University of Padua, Via Loredan, 18, Padova 35131, ItalyTel +39 3473087235Email giulia.grotto.1@studenti.unipd.itBackground: The worldwide prevalence of obstructive lung disease (OLD) is increasing, especially among people > 65 years old, and nearly three in four adults with OLD have two or more comorbid conditions. This study describes the impact of such comorbidities on the healthcare service usage and related costs in a country with universal health coverage, basing on a large cohort of elderly patients with OLD and employing real-world data.Methods: We carried out a retrospective cohort study on a large population of elderly (age > 64 years) patients with OLD served by a Local Health Unit in northern Italy. Their comorbidities were assessed using the clinical diagnoses assigned by the Adjusted Clinical Group (ACG) system to individual patients by combining different information flows. Correlations between number of comorbidities and total annual healthcare service usage and costs were examined with Spearman’s test. Regression models were applied to analyze the associations between the above-mentioned variables, adjusting for age and sex.Results: All types of healthcare service usage (access to emergency care; number of outpatient visits; number of hospital admissions) and pharmacy costs increased significantly with the number of comorbidities. Average total annual costs increased steadily with the number of comorbidities, ranging from € 1158.84 with no comorbidities up to € 9666.60 with 6 comorbidities or more. Poisson regression analyses showed an independent association between the number of comorbidities and the use of every type of healthcare service.Conclusion: These results based on real-world data provide evidence that the burden of care for OLD patients related to their comorbidities is independent of and in addition to the burden related to OLD alone and is strongly dependent on the number of comorbidities, suggesting a holistic approach to multimorbid patients with OLD is the most sound public health strategy.Keywords: comorbidities, obstructive lung disease, healthcare costs, healthcare service usage

The study aimed to test if Briganti’s 2012 nomogram could be associated with the risk of prostate cancer (PCa) progression in European Association of Urology (EAU) intermediate-risk patients treated with robotic surgery. From January 2013 to December 2021, 527 consecutive patients belonging to the EAU intermediate-risk class were selected. Briganti’s 2012 nomogram, which predicts the risk of pelvic lymph node invasion (PLNI), was assessed as a continuous and dichotomous variable that categorized up to the median of 3.0%. Disease progression defined as biochemical recurrence and/or metastatic progression was evaluated by Cox proportional hazards (univariate and multivariate analysis). After a median follow-up of 95.0 months (95% confidence interval [CI]: 78.5–111.4), PCa progression occurred in 108 (20.5%) patients who were more likely to present with an unfavorable nomogram risk score, independently by the occurrence of unfavorable pathology including tumor upgrading and upstaging as well as PLNI. Accordingly, as Briganti’s 2012 risk score increased, patients were more likely to experience disease progression (hazard ratio [HR] = 1.060; 95% CI: 1.021–1.100; P = 0.002); moreover, it also remained significant when dichotomized above a risk score of 3.0% (HR = 2.052; 95% CI: 1.298–3.243; P < 0.0001) after adjustment for clinical factors. In the studied risk population, PCa progression was independently predicted by Briganti’s 2012 nomogram. Specifically, we found that patients were more likely to experience disease progression as their risk score increased. Because of the significant association between risk score and tumor behavior, the nomogram can further stratify intermediate-risk PCa patients, who represent a heterogeneous risk category for which different treatment paradigms exist.

Background and objective: More than 10% of patients with negative clinical metastatic status (cN0M0) on conventional imaging for prostate cancer (PCa) harbor lymph node involvement (pN+) at final pathology following radical prostatectomy (RP) and lymphadenectomy. Our aim was to assess outcomes of initial observation for cN0M0 pN+ PCa and identify prognostic factors that may help in clinical decision-making. Methods: We performed a retrospective multicenter study of patients with cN0M0 PCa on conventional imaging (computed tomography and/or magnetic resonance imaging, and a bone scan) who were found to have pN+ disease at RP between 2000 and 2021. Biochemical recurrence (BCR) and systemic progression/recurrence were the primary outcomes. Kaplan-Meier curves and Cox proportional hazards model were used for survival and multivariate analysis. Key findings and limitations: A total of 469 men were included in this retrospective multicenter trial. Median prostate-specific antigen (PSA) was 10.1 ng/ml (interquartile range [IQR] 6.6–18.0). Among these patients, 56% had grade group ≥4, 53.7% had stage ≥pT3b, 42.6% had positive margins, and 19.6% had PSA persistence. The median number of positive nodes and of nodes removed were 1 (IQR 1–3) and 20 (14–28), respectively. At median follow-up of 41 mo, 48.5% experienced BCR. The 5-yr BCR-free survival rate was 31.7% (95% confidence interval [CI] 26.33–37.1%). Salvage treatments were needed in 211 patients and included radiotherapy (RT; n = 53), RT + androgen deprivation therapy (ADT; n = 88), ADT alone (n = 68), and salvage lymphadenectomy (n = 2). The 5-yr estimated survival rates were 66.3% (95% CI 60.4–72.1) for metastasis-free survival, 97.7% (95% CI 95.5–99.8%) for cancer-specific survival, and 95.3% (95% CI 92.4–98.1%) for overall survival. On multivariable analysis, PSA persistence was an independent predictor of BCR (odds ratio [OR] 51.8, 95% CI 12.2–219.2), exit from observation (OR 8.5, 95% CI 4.4–16.5), and systemic progression (OR 3.0, 95% CI 1.771–4.971). Conclusions: Initial observation in the management of pN+ cN0M0 PCa is feasible and has excellent survival rates in the intermediate term. Patients with worse disease features, especially PSA persistence, have a higher likelihood of recurrence and progression and may be candidates for more aggressive upfront management. Patient summary: We investigated the value of initial observation for men with prostate cancer with negative scan findings for metastasis who were then found to have positive lymph nodes after surgery to remove the prostate. Our results show that initial observation is a good option for patients with less aggressive prostate cancer features.

Abstract Goal contagion, the tendency to adopt others' goals, significantly impacts cognitive processes, which gains particular importance in the emerging field of human–robot interactions. The present study explored how observing human versus robotic actions affects preference and memory. Series of objects undergoing either human or robotic grasping actions together with static (no action) objects were presented, while participants indicated their preference for each object. After a short delay, their memory for grasped, static and new (unstudied) stimuli was tested. Human actions enhanced preference and subsequent recollection of objects, more than robotic actions. In the context of human action, static objects were also perceived as more familiar at recognition. The goal contagion's influence on memory was found to be independent from its impact on preference. These findings highlight the critical role of human interaction in eliciting the impact of goal contagion on cognitive evaluations, memory engagement and the creation of detailed associative memories.

The ‘prison school’ chronotope assumes the dual meaning of ‘school as prison’ and ‘school in prison’. The latter is investigated in this essay, focusing on the narrative strategies, forms, motifs and topoi from which the reality of the Italian penitentiary school is represented, above all through the point of view of writers who have been inside the prison environment in the role of teachers and educators. It also traces the history of this prison school writings particular subgenre, exploring its connections with the political, legal and cultural context, from the Unification of Italy to the present time, integrating literary criticism and the sociology of prison, special education and the pedagogy of deviance, the history of penitentiary law, educational and critical prison studies. Starting from De Amicis and Andreani, among others the works of Invernizzi, Valeriani, Malvezzi, Grimaldi, Albinati, Balzano, Tagliani, Verdone, Trovato, Parrella are studied, finally confirming the prevalence of short and hybrid forms that increasingly characterizes this literary genre.

ABSTRACT Purpose We assessed the prognostic impact of the 2012 Briganti nomogram on prostate cancer (PCa) progression in intermediate-risk (IR) patients presenting with PSA

The article presents the latest results of studies of the events of the Mongol campaign against the Kingdom of Hungary in the Carpathian basin in 1241–42. Compared to the wealth of written sources, archaeology could not provide any serious evidence of the events of the Mongol campaign in Hungary for a long time. The only material evidence of the events of that time was the horizon of sporadic coin finds. However, for a long time there was no clear and reliable evidence of massacres and widespread destruction, which could be judged from the descriptions of contemporaries, often of an apocalyptic nature. Archaeology, studying the period of the Mongol campaigns, the youngest direction of Hungarian archaeology, which has become an independent discipline, and today is one of the most dynamically developing fields. However, it also has required a qualitative improvement in the applied approaches and methods. The full development of the archaeology of the Mongol conquest period is associated with the emergence of new sites as a result of recent targeted and planned studies.

Abstract The inflow of CSF into perivascular spaces (PVS) in the brain is crucial for clearing waste molecules. Inefficiency in PVS flow leads to neurodegeneration. Failure of PVS flushing is associated with CSF flow impairment in the intracranial hydrodynamic condition of CSF hypo-pulsatility. However, enlarged PVS (ePVS), a finding indicative of PVS flow dysfunction, is also present in patients with derangement of CSF dynamics characterized by CSF hyper-pulsatility, which increases CSF flow. Intriguingly, two opposite intracranial hydrodynamic conditions would lead to the same result of impairing the PVS flushing. To investigate this issue, we assessed the subsistence of a dysfunctional interplay between CSF and PVS flows and, if the case, the mechanisms preventing a hyper-pulsatile brain from providing an effective PVS flushing. We analyzed the association between phase contrast MRI aqueductal CSF stroke volume (aqSV), a proxy of CSF pulsatility, and the burden of ePVS in chronic adult hydrocephalus, a disease involving a broad spectrum of intracranial hydrodynamics disturbances. In the 147 (85 males, 62 females) patients, the age at diagnosis ranged between 28 and 88 years (median 73 years). Ninety-seven patients had tri-ventriculomegaly and 50 tetra-ventriculomegaly. According to the extent of ePVS, 113 patients had a high ePVS burden, while 34 had a low ePVS burden. aqSV, which ranged between 0 and 562 μL (median 86 μL), was increased with respect to healthy subjects. Patients presenting with less ePVS burden had higher aqSV (p

ABSTRACT: The sensory characteristics of poultry products are crucial in defining their quality and widely influence consumer choices. Even though the scientific literature clearly indicates that for muscle foods the sensory profile is relevant in purchase decisions and overall acceptability, sensory evaluation has often been underestimated and considered complementary to instrumental and/or chemical assessments. Sensory analysis includes different types of validated tests (discriminative, descriptive, and affective), applied depending on the purpose of the research study, requiring special attention in the sample preparation phase, in particular for nonhomogeneous products such as poultry meat, requiring reproducible cutting, cooking and presentation to the tasters. The aim of this paper is to review, critically assess and discuss sensory methods, standardized procedures and sample preparation tailored for chicken meat, through the literature from 2000 to 2023, with a section dedicated to ethical aspects that must be carefully considered when designing a sensory protocol. The target readers are both the research and the business communities, as the information can be widely applied for quality control, to develop new food products, to understand or drive preferences or, for example, to assess potential sensory differences among chickens fed with different diets. To the best of the authors' knowledge, this review represents a useful first guide for those approaching the sensory analysis of chicken meat.

Objetivo: No projeto de extensão “Doe vida: conscientização e incentivo à doação de órgãos e medula óssea”, da Universidade Estadual de Maringá, o objetivo foi conscientizar a população sobre a importância do transplante de medula óssea, desmistificar conceitos equivocados e incentivar a adesão ao cadastro em eventos públicos. Metodologia: No ano de 2024 foram realizadas campanhas de conscientização em dois eventos públicos, sendo eles a “Exposição Feira Agropecuária, Industrial e Comercial de Maringá (Expoingá)” e “Arena Sustentável Maringá”. As atividades desempenhadas nas campanhas foram realizadas por discentes de graduação e pós-graduação da UEM, acompanhadas da coordenadora. Foram utilizadas as seguintes estratégias: apresentação de vídeos explicativos, distribuição de folders, exposição de peças anatômicas de ossos, realização de dinâmicas interativas com premiações. Foi realizado a atualização do cadastro no no Registro Brasileiro de Doadores Voluntários de Medula Óssea (REDOME). Resultados: A abordagem adotada mostrou-se eficaz, gerando grande interesse do público. Constatamos que grande parcela da população possui informações incorretas sobre o tema, porém demonstram empatia e desejo de se cadastrarem como doadores de medula óssea. A presença de doadores já cadastrados também foi significativa, reforçando a relevância de campanhas contínuas de conscientização. Discussão: Para atrair a população, utilizamos as peças anatômicas, a maior dificuldade encontrada na realização das campanhas é expor o assunto de forma simples, particularmente para o público infanto-juvenil. A maioria dos adultos apresentaram informações incorretas e/ou desatualizadas. Nos casos descritos acima, esclarecemos as dúvidas e fornecemos materiais. Quanto à atualização de cadastros no REDOME, diversos doadores comentaram que o processo é rápido, fácil e que não lembram de realizar o processo em casa. Conclusão: As campanhas em eventos sociais são uma estratégia eficaz para alcançar um público diversificado, incluindo pessoas com diferentes idades, níveis de escolaridade, etnias e classes sociais, promovendo uma maior captação de doadores voluntários de medula óssea, no entanto, reforça-se a necessidade de traçar estratégias atrativas, adequadas para cada grupo etário a fim de atingir o objetivo do projeto.

Resumo Fundamento: A fibrilação atrial (FA) é a arritmia cardíaca sustentada mais frequente, mas ainda é subdiagnosticada especialmente em pacientes assintomáticos. Objetivo: Avaliar uma estratégia simples para otimizar a identificação da FA. Métodos: Avaliados indivíduos assintomáticos com 65 anos ou mais, portadores de hipertensão arterial (HA) ou insuficiência cardíaca (IC). Os dados foram inseridos e armazenados em plataforma REDCap. Inicialmente foram realizadas análise de risco de FA com o algoritmo matemático Stroke Risk Analysis (SRA) aplicado em eletrocardiograma (ECG) de 1 hora. Todos os pacientes de alto risco de FA foram orientados a realizar o protocolo de ECG domiciliar por sete dias com o equipamento portátil Kardia 6L OMRON, AliveCor®. O teste de Kolmogorov-Smirnov foi usado para verificar a normalidade da distribuição das variáveis quantitativas; aquelas com distribuição normal foram expressas em média e desvio-padrão. Adotou-se como significativo o valor de p

ABSTRACT Purpose: To evaluate exogenous hyaluronic acid (HA) derived from bacterial fermentation through enteral and parenteral routes in ischemic skin flaps induced in rats, using clinical and histological exams; and interleukins (IL) as tissue inflammatory biomarkers. Methods: Sixty-four male adults Wistar rats with ischemic skin flaps on the dorsum were randomized into four groups, based on the treatment protocol: subcutaneous administration of saline solution (0.9%) (GI); oral administration of distilled water (GII); subcutaneous administration of HA (0.3%) (GIII); and oral administration of HA (1%) (GIV). Flaps of all groups were comparable regarding clinical and macroscopic evaluation, histological examination, pro-inflammatory cytokines (IL-1β, IL-6, and tumor necrosis factor-α) and anti-inflammatory cytokine IL-10. Results: A lower percentage of necrosis was identified in flaps treated with subcutaneous administration of HA (0.3%). The pro- and anti-inflammatory cytokines, epidermis thickness, blood vessels, and inflammatory cells showed statistically significant inter-group and intra-group differences (p < 0.05). Conclusions: High molecular HA (1,400 ~ 2,000 kDa) administrated by subcutaneous or oral route exhibited beneficial effects in ischemic skin flaps of rats. However, subcutaneous administration of HA (0.3%) showed better results in terms of the percentage of necrosis and epithelialization.

Climate change impacts food systems, causing nutritional deficiencies and increasing cardiovascular diseases (CVD). Regulatory frameworks like the European Farm-to-Fork Strategy aim to mitigate these effects, but current EU food safety regulations inadequately address health risks from poor diet quality and contaminants.Climate change adversely affects food quality, such as nutrient depletion in crops due to higher CO2 levels, leading to diets that promote chronic diseases, including CVD. Women, because of their roles in food production and their unique physiological responses to nutrients, face distinct vulnerabilities. This review explores the interplay between climate change, diet, and cardiovascular health in women. The review highlights that sustainable diets, particularly the Mediterranean diet, offer health benefits and lower environmental impacts but are threatened by climate change-induced disruptions. Women's adherence to the Mediterranean diet is linked to significant reductions in CVD risk, though sex-specific responses need further research.Resilient agricultural practices, efficient water management, and climate-smart farming are essential to mitigate climate change's negative impacts on food security. Socio-cultural factors influencing women's dietary habits, such as traditional roles and societal pressures, further complicate the picture.Effective interventions must be tailored to women, emphasizing education, community support, policy changes, and media campaigns promoting healthy eating. Collaborative approaches involving policymakers, health professionals, and the agricultural sector are crucial for developing solutions that protect public health and promote sustainability.Addressing the multifaceted challenges posed by climate change to food quality and cardiovascular health in women underscores the need for integrated strategies that ensure food security, enhance diet quality, and mitigate environmental impacts.

In implantology, among the key choices, to obtain predictable results, it is essential to establish, using cone beam computed tomography (CBCT), the bone site and where to insert the implants; during the surgical phase, these sites must be identified on the oral mucosa. Surgical templates are a valid aid, especially in complex cases which require the insertion of more than three or four implants. In cases of a single implant, on the other hand, surgical guides are rarely used, and the implant is often inserted freehand; therefore, the identification of the implant site on the oral mucosa (after choosing the location on the CBCT) is more difficult. For this reason, the clinician uses the teeth in the arch as a reference. This study evaluates the ability of the human eye to identify, on the oral mucosa, where the implant collars will be positioned, the position of which has previously been chosen on the CBCT, in cases where the hands-free surgical technique (without surgical guides) is used. The verification of this precision is carried out using particular thermo-printed templates which contain radiopaque metal spheres. The results show that, in the freehand technique, it is difficult to precisely identify the implant sites (chosen via X-ray) on the mucosa, especially when they are far from natural teeth adjacent to the edentulous area. In case of monoedentulism, the freehand implant technique seems to be applicable by expert implantologists with a reduced risk of error; in fact, clinical experience helps to find the correct correspondence between the implant site chosen on the CBCT and its identification on the mucosa. The level of experience is fundamental in the clinician’s decision about whether or not to use surgical guides; in fact, doctors with little experience should use surgical guides even in the simplest cases to reduce the risk of error.

Abstract Hematopoietic stem cell gene therapy (GT) using a γ-retroviral vector (γ-RV) is an effective treatment for Severe Combined Immunodeficiency due to Adenosine Deaminase deficiency. Here, we describe a case of GT-related T-cell acute lymphoblastic leukemia (T-ALL) that developed 4.7 years after treatment. The patient underwent chemotherapy and haploidentical transplantation and is currently in remission. Blast cells contain a single vector insertion activating the LIM-only protein 2 (LMO2) proto-oncogene, confirmed by physical interaction, and low Adenosine Deaminase (ADA) activity resulting from methylation of viral promoter. The insertion is detected years before T-ALL in multiple lineages, suggesting that further hits occurred in a thymic progenitor. Blast cells contain known and novel somatic mutations as well as germline mutations which may have contributed to transformation. Before T-ALL onset, the insertion profile is similar to those of other ADA-deficient patients. The limited incidence of vector-related adverse events in ADA-deficiency compared to other γ-RV GT trials could be explained by differences in transgenes, background disease and patient’s specific factors.

Abstract Background The occurrence of higher winter temperatures in Brazilian areas with tropical and highland climates may result in a fifth peak of tick populations during winter in addition to the four generations previously described. Therefore, a strategic control protocol was developed with treatments in two seasons with the objective of controlling the generations of ticks that occur in spring/summer and those that occur in autumn/winter. Methods The study was conducted in Mato Grosso do Sul, Brazil, from the beginning of the rainy season, November 2020, to October 2021. In a randomized block design, 36 calves were distributed into three groups: (i) negative control; (ii) traditional strategic control in one season (SC1S), at the beginning of the rainy season; and (iii) strategic control in two seasons (SC2S), at the beginning and end of the rainy season. The SC1S strategic control group was treated on day 0, November 2020, and twice more with intervals of 42 days. The SC2S group received three more treatments beginning on day 182, May 2021, with intervals of 42 days. All treatments consisted of 5% fluralaner (Exzolt® 5%) delivered via a pour-on dose of 1 mL/20 kg body weight. Counts of semi-engorged female ticks were performed on day 3 and every 14 days thereafter, and the animals were weighed at the same time. Results Fluralaner showed a mean efficacy of more than 95% up to day 294. The two treated groups showed a decrease (P 0.05) from those of the control group from day 231 onward. The final mean weight gain of each group was 76.40 kg, 98.63 kg, and 115.38 kg for the control, SC1S, and SC2S groups, respectively, differing (P

Abstract Background In Rio Grande do Sul, Brazil, a region with a subtropical climate, Rhipicephalus microplus is present in taurine cattle raised for beef and milk. In addition, ticks resistant to multiple acaricides are present in this region. Recently, fluralaner (isoxazoline) was launched on the market. Thus, there is a need to evaluate the effects of fluralaner for the control of R. microplus on taurine cattle. In addition, occurrence of myiasis by Cochliomyia hominivorax larvae after tick parasitism and weight gain of cattle during the experimental period were evaluated. Methods Thirty naturally infested cattle were divided into two experimental groups: T01, treated with fluralaner (2.5 mg/kg) pour-on; T02, control. T01 received fluralaner on Days 0 (early summer in January), 42 and 84 (early autumn), whereas T02, a control group, received palliative treatment with a spray formulation when the group mean was ≥ 30 ticks. Counts of R. microplus females and calculation of the efficacy of fluralaner were performed on Days 3, 7, 14, 28, 35, 42, 56, 70, 84, 98, 112 and 126. The occurrence of myiasis was assessed throughout the study period. In addition, the weight, weight gain and daily weight gain of the animals were evaluated. Results In the 12 evaluations performed, the parasitic load of T01 was near zero. Fluralaner showed 99.5% efficacy on the 3rd day after the first treatment and 100% efficacy from Day 7 to Day 126. Cochliomyia hominivorax larvae (n = 6; p = 0.0251) were found only in the control group (T02). At the end of the study, the animals subjected to treatments with fluralaner gained 32.8 kg more than the animals in the control group. Conclusions Application of fluralaner in summer and autumn, with 42-day intervals between treatments, was effective to control R. microplus on taurine cattle, which also gained more weight than control cattle. Additionally, no cases of myasis were documented in animals treated with fluralaner. Graphical Abstract

Abstract Cyanobacterial phycocyanin and phycoerythrin are gaining commercial interest due to their nutrition and healthcare values. This research analyzed the biomass accumulation and pigment production of two strains of Leptolyngbya under different combinations of light colors and intensities. The results showed that while Leptolyngbya sp.4 B1 (B1) produced all phycobiliproteins, Leptolyngbya sp.5 F2 (F2) only had phycocyanin and allophycocyanin. Both the color of the light and its light intensity affect the biomass accumulation and phycoerythrin concentration in strain B1. Although white light at medium intensity (50 μmol m-2 s-1) causes greater biomass accumulation (1.66 ± 0.13 gDW L-1), low-intensity (25 μmol m-2 s-1) green light induces lower biomass accumulation with twice the pigment content (87.70 ± 2.46 mg gDW -1), culminating in 71% greater productivity. In contrast, for the F2 strain, light intensity positively influenced biomass and pigment accumulation, being observed 2.25 ± 0.10 gDW L-1 under white light at 100 μmol m-2 s-1 and higher phycocyanin concentration (138.38 ± 3.46 mg gDW -1) under red light at 100 μmol m-2 s-1. These findings provide insights into optimizing the growth conditions by altering the intensity and wavelength of light for future production of phycocyanin and phycoerythrin from local cyanobacteria.

Teaching gender and sex differences is fundamental in medical classes because it has a strong impact in reducing disparity in treatment, in defining effective and personalized therapies that respect the different physiology and pathophysiology of women. Furthermore, it is the prerequisite for the pharmacoequity.

Abstract Background The present study aimed to evaluate the effects of different treatment strategies using a new commercial formulation containing pour-on fluralaner on Rhipicephalus microplus infestation in cattle and in pastures in a tropical climate region where up to five generations of this tick species can occur per year. Methods Forty-five naturally infested cattle were divided into three experimental groups: T01, treated with fluralaner (2.5 mg/kg) pour-on every 42 days; T02, the cattle received the first treatment with fluralaner on Day 0 but the next treatment involved a weekly visual evaluation; T03, control, received palliative treatment with a spray formulation when the group mean was ≥ 30 ticks. Counts of female R. microplus were performed weekly until day 343, and larval counts on pasture were performed on Days 0, 30, and 60 and every 30 days until Day 330. Results Using fluralaner, six applications were performed in Group T01, and four were performed in Group T02. In the control group (T03), it was necessary to perform eight palliative acaricide treatments with the spray formulation. The animals in T01 and T02 showed lower mean tick counts (p ≤ 0.05) than the control group (T03) on 28 and 27 of the 49 evaluated dates, respectively. In the paddock where the animals were kept as controls, the R. microplus larvae counts increased to 1458. In the paddocks where the animals were treated with fluralaner, the number was ≤ 19 per paddock during the study. Conclusions The different strategic treatment protocols performed with pour-on fluralaner (2.5 mg/kg) over a year in taurine cattle in a tropical region with a history of up to five annual generations of cattle ticks were effective, maintaining levels of R. microplus infestations in animals and in pastures close to zero in most of the study. Depending on the retreatment criterion adopted, the number of applications per year may be lower, resulting in a reduction in the mean cost of acaricide treatment per year and lower exposure of R. microplus populations to the active ingredient, resulting in lower resistance and selection pressure. Graphical Abstract

Abstract Professorships in Italy are assigned following public competitions. However, favouritism affects faculty hiring. Researchers lacking clientelistic support remain excluded from academia and are obliged to seek employment abroad or at non-university institutions, or to abandon their career. Do non-recruited researchers have better or worse scientific capacity than those who have attained professorships in Italy? Files regarding the competitions in bibliometric disciplines won by 186 professors in Florence were analysed. An equal number of professors recruited at other Italian universities and scientists who never attained professorship in Italy were randomly drawn from the pool of individuals having national scientific qualification (the prerequisite for professorship) in the same disciplines as each Florentine professor. H-indexes of the year of qualification (T1), of the Florence call (T2), and in July 2021 (T3) were obtained from Scopus. Non-recruited individuals were more likely (Chi-square test) to show a higher H-index than both Florentine (T1 p = 0.0005, T2 p = 0.0015, T3 p = 0.0095) and non-Florentine professors (T1 p = 0.0078, T2 p = 0.0245, T3 p = 0.0500). Fifty-four non-recruited scientists serve in foreign universities, 100 at national/international research centres. The remaining scientists (25 who continue producing despite precarious employment, and seven who have stopped publishing) were as likely as Florentine (T3 p = 0.69) and non-Florentine (T3 p = 0.14) professors to show a higher H-index. Italian faculty hiring disregards merit. A more challenging qualification would limit the access of researchers with lower scientific capacity, and favour those with greater proficiency. As it stands, competition is useless. Once professors obtain permanent employment, they seem less motivated to publish.

Professorships in Italy are assigned following public competitions. However, favouritism affects faculty hiring. Researchers lacking clientelistic support remain excluded from academia and are obliged to seek employment abroad or at non-university institutions, or to abandon their career. Do non-recruited researchers have better or worse scientific capacity than those who have attained professorships in Italy? Files regarding the competitions in bibliometric disciplines won by 186 professors in Florence were analysed. An equal number of professors recruited at other Italian universities and scientists who never attained professorship in Italy were randomly drawn from the pool of individuals having national scientific qualification (the prerequisite for professorship) in the same disciplines as each Florentine professor. H-indexes of the year of qualification (T1), of the Florence call (T2), and in July 2021 (T3) were obtained from Scopus. Non-recruited individuals were more likely (Chi-square test) to show a higher H-index than both Florentine (T1 p = 0.0005, T2 p = 0.0015, T3 p = 0.0095) and non-Florentine professors (T1 p = 0.0078, T2 p = 0.0245, T3 p = 0.0500). Fifty-four non-recruited scientists serve in foreign universities, 100 at national/international research centres. The remaining scientists (25 who continue producing despite precarious employment, and seven who have stopped publishing) were as likely as Florentine (T3 p = 0.69) and non-Florentine (T3 p = 0.14) professors to show a higher H-index. Italian faculty hiring disregards merit. A more challenging qualification would limit the access of researchers with lower scientific capacity, and favour those with greater proficiency. As it stands, competition is useless. Once professors obtain permanent employment, they seem less motivated to publish.