1. A dual-site fluorescent probe for the detection of γ-glutamyl transpeptidase activity and its application in garlic.
- Author
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Ding L, Wu X, Yang S, Tian H, and Sun B
- Subjects
- Humans, Spectrometry, Fluorescence methods, Enzyme Assays methods, Garlic chemistry, Garlic enzymology, gamma-Glutamyltransferase metabolism, gamma-Glutamyltransferase analysis, Fluorescent Dyes chemistry
- Abstract
Developing convenient γ-glutamyl transpeptidase (GGT) activity detection methods is of great significance for soaking Laba garlic and human diseases detection. A dual-site fluorescent probe (probe 1) was developed for detection the activity of GGT. Probe 1 could recognize GGT by the enzymatic hydrolysis of peptide bond by GGT. There has a linear relationship between the fluorescence intensity of probe 1 at 416 nm and the activity of GGT. And the color of the probe solution gradually changed from colorless to blue with the increase of GGT activity under 365 nm ultraviolet light. Importantly, it has a linear relationship between the activity of GGT and the blue (B) value of probe solution photo. Therefore, probes can serve as a convenient tool for detecting GGT activity. More importantly, the probe has been successfully applied to detect of GGT activity in garlic., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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