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Studies were conducted on the incidence of major pests viz. serpentine leaf miner, spider mite, leafhopper, and tobacco cutworm of pole bean in relations to the crop spacing and fertilizer application, during rabi 2019-20. Spacing between crops and nutrient nourishment distinctively governed the activity of insect pests in pole bean crop (Phaseolus vulgaris L.), grown under the net house. It was revealed from the study that when the crop was raised at a wider spacing of 45×60 cm with fertigation of 125% RDF (Recommended dose of fertilizer) (78.12:125:93.75 NPK kg per ha) resulted in the lowest population density of the infesting insect whereas the highest at the interaction of 45×30 cm spacing and 75% RDF (46.87:75:56.25 NPK kg per ha). This suggested that a wider spacing and increased dose of recommended fertilizer could lessen the pest population and vice-versa conditions could increase it. Hence it can be a non-chemical implication for the management of insect-pest under the net house. [ABSTRACT FROM AUTHOR]

Field experiments were conducted during 2011-12, at Agricultural Research Station, UAS, Dharwad, Karnataka, South India to know the efficacy of new molecules and botanicals against chilli pests. viz., green peach aphid (Myzys persicae Sulzer, Aphis gossypi Glover), thrips (Scirtothrips dorsalis Hood), yellow mite (Polyphagotarsonemus latus Banks) and fruit borer (Helicoverpa armigera Hubner). The results revealed that thiamethoxam 25WG @ 1 g/l, abamectin 1.8EC @ 0.5ml/l, diafenthiuron 50 WP @ 0.75g/l were highly effective for the management of chilli sucking pests. Against H. armigera, novaluron 10 EC @ 0.75ml/l, emamectin benzoate 5SG @ 0.4g/l and spinosad45SC @ 0.3ml/l were found quite promising. The pesticides from biological origin and neem based formulations might be relatively less harmful to the natural enemies than insecticide like imidacloprid and cypermethrin. Significantly increased yield (4.65 q/ha) was recorded in diafenthiuron 50 WP @ 0.75g/l with higher net returns (Rs 22,661/ ha). [ABSTRACT FROM AUTHOR]

Field experiments were conducted during kharif 2010-11 and 2011-12 at Agricultural Research Station, UAS, Dharwad, Karnataka, South India to assess the effect of border and barrier cropping for the management of chilli pests. Chilli as border and barrier crop with South African tall maize and sweet sorghum was found to be superior over sole crop by recording the lowest population of sucking pests, leaf curl index, larval population of H. armigera, fruit damage and significantly higher growth parameters as well as dry chili yield (3.95 and 3.89q/ha) with a net return of Rs 19,528/- and C:B ratio 1: 2.56. Activity of predators such as coccinellids and chrysopids were found to be greatly distributed in plots having border and barrier crops. [ABSTRACT FROM AUTHOR]

Background: Cancer stem cells exhibit close resemblance to normal stem cells in phenotype as well as function. Hence, studying normal stem cell behavior is important in understanding cancer pathogenesis. It has recently been shown that human breast stem cells can be enriched in suspension cultures as mammospheres. However, little is known about the behavior of these cells in long-term cultures. Since extensive self-renewal potential is the hallmark of stem cells, we undertook a detailed phenotypic and functional characterization of human mammospheres over long-term passages., Methodology: Single cell suspensions derived from human breast 'organoids' were seeded in ultra low attachment plates in serum free media. Resulting primary mammospheres after a week (termed T1 mammospheres) were subjected to passaging every 7th day leading to the generation of T2, T3, and T4 mammospheres., Principal Findings: We show that primary mammospheres contain a distinct side-population (SP) that displays a CD24(low)/CD44(low) phenotype, but fails to generate mammospheres. Instead, the mammosphere-initiating potential rests within the CD44(high)/CD24(low) cells, in keeping with the phenotype of breast cancer-initiating cells. In serial sphere formation assays we find that even though primary (T1) mammospheres show telomerase activity and fourth passage T4 spheres contain label-retaining cells, they fail to initiate new mammospheres beyond T5. With increasing passages, mammospheres showed an increase in smaller sized spheres, reduction in proliferation potential and sphere forming efficiency, and increased differentiation towards the myoepithelial lineage. Significantly, staining for senescence-associated beta-galactosidase activity revealed a dramatic increase in the number of senescent cells with passage, which might in part explain the inability to continuously generate mammospheres in culture., Conclusions: Thus, the self-renewal potential of human breast stem cells is exhausted within five in vitro passages of mammospheres, suggesting the need for further improvisation in culture conditions for their long-term maintenance.