249 results on '"Guo-Yu Yang"'
Search Results
2. Pseudorabies virus hijacks the Rab6 protein to promote viral assembly and egress
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Dong-Ge Liang, Yu-Kun Guo, Shi-Bo Zhao, Guo-Yu Yang, Ying-Qian Han, Bei-Bei Chu, and Sheng-Li Ming
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PRV ,small GTPase ,Rab6 ,gB ,gE ,viral assembly and egress ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Pseudorabies virus (PRV) is recognized as the aetiological agent responsible for Aujeszky’s disease, or pseudorabies, in swine populations. Rab6, a member of the small GTPase family, is implicated in various membrane trafficking processes, particularly exocytosis regulation. Its involvement in PRV infection, however, has not been documented previously. In our study, we observed a significant increase in the Rab6 mRNA and protein levels in both PK-15 porcine kidney epithelial cells and porcine alveolar macrophages, as well as in the lungs and spleens of mice infected with PRV. The overexpression of wild-type Rab6 and its GTP-bound mutant facilitated PRV proliferation, whereas the GDP-bound mutant form of Rab6 had no effect on viral propagation. These findings indicated that the GTPase activity of Rab6 was crucial for the successful spread of PRV. Further investigations revealed that the reduction in Rab6 levels through knockdown significantly hampered PRV proliferation and disrupted virus assembly and egress. At the molecular level, Rab6 was found to interact with the PRV glycoproteins gB and gE, both of which are essential for viral assembly and egress. Our results collectively suggest that PRV exploits Rab6 to expedite its assembly and egress and identify Rab6 as a promising novel target for therapeutic treatment for PRV infection.
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- 2024
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3. Porcine reproductive and respiratory syndrome virus 2 hijacks CMA-mediated lipolysis through upregulation of small GTPase RAB18.
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Guo-Li Li, Ying-Qian Han, Bing-Qian Su, Hai-Shen Yu, Shuang Zhang, Guo-Yu Yang, Jiang Wang, Fang Liu, Sheng-Li Ming, and Bei-Bei Chu
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Immunologic diseases. Allergy ,RC581-607 ,Biology (General) ,QH301-705.5 - Abstract
RAB GTPases (RABs) control intracellular membrane trafficking with high precision. In the present study, we carried out a short hairpin RNA (shRNA) screen focused on a library of 62 RABs during infection with porcine reproductive and respiratory syndrome virus 2 (PRRSV-2), a member of the family Arteriviridae. We found that 13 RABs negatively affect the yield of PRRSV-2 progeny virus, whereas 29 RABs have a positive impact on the yield of PRRSV-2 progeny virus. Further analysis revealed that PRRSV-2 infection transcriptionally regulated RAB18 through RIG-I/MAVS-mediated canonical NF-κB activation. Disrupting RAB18 expression led to the accumulation of lipid droplets (LDs), impaired LDs catabolism, and flawed viral replication and assembly. We also discovered that PRRSV-2 co-opts chaperone-mediated autophagy (CMA) for lipolysis via RAB18, as indicated by the enhanced associations between RAB18 and perlipin 2 (PLIN2), CMA-specific lysosomal associated membrane protein 2A (LAMP2A), and heat shock protein family A (Hsp70) member 8 (HSPA8/HSC70) during PRRSV-2 infection. Knockdown of HSPA8 and LAMP2A impacted on the yield of PRRSV-2 progeny virus, implying that the virus utilizes RAB18 to promote CMA-mediated lipolysis. Importantly, we determined that the C-terminal domain (CTD) of HSPA8 could bind to the switch II domain of RAB18, and the CTD of PLIN2 was capable of associating with HSPA8, suggesting that HSPA8 facilitates the interaction between RAB18 and PLIN2 in the CMA process. In summary, our findings elucidate how PRRSV-2 hijacks CMA-mediated lipid metabolism through innate immune activation to enhance the yield of progeny virus, offering novel insights for the development of anti-PRRSV-2 treatments.
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- 2024
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4. RhoA suppresses pseudorabies virus replication in vitro
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Xin-Man Li, Shi-Ping Wang, Jin-Yuan Wang, Ting Tang, Bo Wan, Lei Zeng, Jiang Wang, Bei-Bei Chu, Guo-Yu Yang, and Jia-Jia Pan
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RhoA ,Actin cytoskeleton ,Viral replication ,Pseudorabies virus ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract The porcine pseudorabies virus (PRV) is one of the most devastating pathogens and brings great economic losses to the swine industry worldwide. Viruses are intracellular parasites that have evolved numerous strategies to subvert and utilize different host processes for their life cycle. Among the different systems of the host cell, the cytoskeleton is one of the most important which not only facilitate viral invasion and spread into neighboring cells, but also help viruses to evade the host immune system. RhoA is a key regulator of cytoskeleton system that may participate in virus infection. In this study, we characterized the function of RhoA in the PRV replication by chemical drugs treatment, gene knockdown and gene over-expression strategy. Inhibition of RhoA by specific inhibitor and gene knockdown promoted PRV proliferation. On the contrary, overexpression of RhoA or activation of RhoA by chemical drug inhibited PRV infection. Besides, our data demonstrated that PRV infection induced the disruption of actin stress fiber, which was consistent with previous report. In turn, the actin specific inhibitor cytochalasin D markedly disrupted the normal fibrous structure of intracellular actin cytoskeleton and decreased the PRV replication, suggesting that actin cytoskeleton polymerization contributed to PRV replication in vitro. In summary, our data displayed that RhoA was a host restriction factor that inhibited PRV replication, which may deepen our understanding the pathogenesis of PRV and provide further insight into the prevention of PRV infection and the development of anti-viral drugs.
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- 2023
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5. Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry.
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Bing-Qian Su, Guo-Yu Yang, Jiang Wang, Sheng-Li Ming, and Bei-Bei Chu
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Immunologic diseases. Allergy ,RC581-607 ,Biology (General) ,QH301-705.5 - Abstract
Viral infection is a significant risk factor for fertility issues. Here, we demonstrated that infection by neurotropic alphaherpesviruses, such as pseudorabies virus (PRV), could impair female fertility by disrupting the hypothalamus-pituitary-ovary axis (HPOA), reducing progesterone (P4) levels, and consequently lowering pregnancy rates. Our study revealed that PRV exploited the transient receptor potential mucolipin 1 (TRPML1) and its lipid activator, phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2), to facilitate viral entry through lysosomal cholesterol and Ca2+. P4 antagonized this process by inducing lysosomal storage disorders and promoting the proteasomal degradation of TRPML1 via murine double minute 2 (MDM2)-mediated polyubiquitination. Overall, the study identifies a novel mechanism by which PRV hijacks the lysosomal pathway to evade P4-mediated antiviral defense and impair female fertility. This mechanism may be common among alphaherpesviruses and could contribute significantly to their impact on female reproductive health, providing new insights for the development of antiviral therapies.
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- 2024
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6. The glycoprotein 5 of porcine reproductive and respiratory syndrome virus stimulates mitochondrial ROS to facilitate viral replication
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Shuang Zhang, Lei Zeng, Bing-Qian Su, Guo-Yu Yang, Jiang Wang, Sheng-Li Ming, and Bei-Bei Chu
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PRRSV ,ER-mitochondria contacts ,IP3R ,VDAC1 ,autophagy ,NLRP3 inflammasome ,Microbiology ,QR1-502 - Abstract
ABSTRACTViruses have evolved sophisticated mechanisms to manipulate host cell organelles to serve as niches for persistence and proliferation. In the present study, we aimed to investigate the role of cellular organelles in the replication of porcine reproductive and respiratory syndrome virus (PRRSV). We found that the morphology of mitochondria and the endoplasmic reticulum (ER) were both altered, and the contact between these two organelles was enhanced during PRRSV infection. By the overexpression of PRRSV-encoded open reading frames, we identified that only glycoprotein 5 (GP5) was essential for ER-mitochondria contact. Further investigation revealed that GP5 interacted with the ER inositol 1,4,5-triphosphate receptor (IP3R) and the mitochondrial voltage-dependent anion channel (VDAC1) to promote the Ca2+ efflux from ER into mitochondria. Excessive mitochondrial Ca2+ uptake resulted in mitochondrial dysfunction and substantial mitochondrial reactive oxygen species (mROS) production. Elevated mROS activated autophagy through the AMPK/mROR/ULK1 axis to facilitate PRRSV replication. GP5-induced mROS also triggered the NOD-like receptor family pyrin domain-containing protein 3 (NLRP3) inflammasome. Inhibition of autophagy augmented NLRP3 inflammasome activation and exhibited an anti-PRRSV effect, suggesting autophagy counteracted the NLRP3-mediated innate immune response. Overall, our findings highlighted the importance of cellular organelles in virus-host interactions and provided new insights into the complex interplay between virus replication and innate immune responses.IMPORTANCEPorcine reproductive and respiratory syndrome virus (PRRSV) presents a significant economic concern for the global swine industry due to its connection to serious production losses and increased mortality rates. There is currently no specific treatment for PRRSV. Previously, we had uncovered that PRRSV-activated lipophagy to facilitate viral replication. However, the precise mechanism that PRRSV used to trigger autophagy remained unclear. Here, we found that PRRSV GP5 enhanced mitochondrial Ca2+ uptake from ER by promoting ER-mitochondria contact, resulting in mROS release. Elevated mROS induced autophagy, which alleviated NLRP3 inflammasome activation for optimal viral replication. Our study shed light on a novel mechanism revealing how PRRSV exploits mROS to facilitate viral replication.
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- 2023
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7. New {Co9} cluster-added banana-shaped polyoxometalate modified by B atom
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Hai-Lou Li and Guo-Yu Yang
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polyoxometalate (pom) ,co-added pom ,banana-shaped structure ,hydrothermal synthesis ,Inorganic chemistry ,QD146-197 - Abstract
In this study, a new {Co9} cluster-added polyoxometalate (POM) Cs3K4Na5H6[BO(OH)2Co9O(OH)(H2O)3(GeW6O26)(B-α-GeW9O34)2]·22H2O (1) was successfully synthesized in the presence of inorganic boron sources under hydrothermal conditions. Its structural characterizaiton is realized using single-crystal X-ray diffraction, infrared spectroscopy, thermogravimetric analysis, and powder X-ray diffraction. Compound 1 represents the first Co-added POM bonded by a B atom, and its polyoxoanion is constructed by fusing together with one hexalacunary GeW6O26 and two trilacunary B-α-GeW9O34 fragments in an unprecedented V-shaped {Co9} cluster. Additionally, compound 1 is an efficient catalyst for accelerating the Knoevenagel condensation of various aldehydes with malonitrile.
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- 2023
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8. Synthesis, structure, and characteristics of a hexa-Ti-oxo-cluster-added Keggin-type polyoxometalate
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Yu Wang, Hai-Lou Li, and Guo-Yu Yang
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ti-oxo cluster ,addition reaction ,hydrothermal synthesis ,nonlinear optics ,Inorganic chemistry ,QD146-197 - Abstract
An acentric hexa-Ti-oxo-cluster-added trimeric phosphotungstate, [H2N(CH3)2]3H12[Ti6O6(A-α-1,2-PW10O37)3]∙12 H2O (1), was synthesized by reacting trivacant polyoxometalate (POM) fragments with Ti4+ ions under hydrothermal conditions and characterized via single-crystal/powder X-ray diffraction, solid UV–vis and IR spectroscopies, and thermogravimetric analysis. The polyoxoanion of 1 is constituted by three Ti2O3(A-α-1,2-PW10O37) subunits linked through Ti–O–Ti bonds, forming a ring-shaped configuration. Furthermore, 1 exhibits a second-harmonic generation response about 0.6 times that of KH2PO4, which paves the way toward the development of Ti-added POMs in the field of nonlinear optical materials.
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- 2023
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9. Two New Aluminoborates with 3D Porous-Layered Frameworks
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Chen Wang, Juan Chen, Chong-An Chen, Zhen-Wen Wang, and Guo-Yu Yang
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aluminoborates ,oxoboron cluster ,3D porous layers ,solvothermal syntheses ,Organic chemistry ,QD241-441 - Abstract
Two new aluminoborates, NaKCs[AlB7O13(OH)]·H2O (1) and K4Na5[AlB7O13(OH)]3·5H2O (2), have been hydro(solvo)thermally made with mixed alkali metal cationic templates. Both 1 and 2 crystallize in the monoclinic space group P21/n and contain similar units of [B7O13(OH)]6− cluster and AlO4 tetrahedra. The [B7O13(OH)]6− cluster is composed of three classical B3O3 rings by vertex sharing, of which two of them connect with AlO4 tetrahedra to constitute monolayers, and one provides an O atom as a bridging unit to link two oppositely orientated monolayers by Al-O bonds to form 3D porous-layered frameworks with 8-MR channels. UV-Vis diffuse reflectance spectra indicate that both 1 and 2 exhibit short deep-UV cutoff edges below 190 nm, revealing that they have potential applications in deep-UV regions.
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- 2023
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10. CRISPR/Cas9-based generation of a recombinant double-reporter pseudorabies virus and its characterization in vitro and in vivo
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Peng-Fei Fu, Xuan Cheng, Bing-Qian Su, Li-Fang Duan, Cong-Rong Wang, Xin-Rui Niu, Jiang Wang, Guo-Yu Yang, and Bei-Bei Chu
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Pseudorabies virus ,Firefly luciferase ,EGFP ,CRISPR/Cas9 ,Imaging in vivo ,25-hydroxycholesterol ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Pseudorabies, caused by pseudorabies virus (PRV) variants, has broken out among commercial PRV vaccine-immunized swine herds and resulted in major economic losses to the pig industry in China since late 2011. However, the mechanism of virulence enhancement of variant PRV is currently unclear. Here, a recombinant PRV (rPRV HN1201-EGFP-Luc) with stable expression of enhanced green fluorescent protein (EGFP) and firefly luciferase as a double reporter virus was constructed on the basis of the PRV variant HN1201 through CRISPR/Cas9 gene-editing technology coupled with two sgRNAs. The biological characteristics of the recombinant virus and its lethality to mice were similar to those of the parental strain and displayed a stable viral titre and luciferase activity through 20 passages. Moreover, bioluminescence signals were detected in mice at 12 h after rPRV HN1201-EGFP-Luc infection. Using the double reporter PRV, we also found that 25-hydroxycholesterol had a significant inhibitory effect on PRV both in vivo and in vitro. These results suggested that the double reporter PRV based on PRV variant HN1201 should be an excellent tool for basic virology studies and evaluating antiviral agents.
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- 2021
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11. EGCG Restricts PRRSV Proliferation by Disturbing Lipid Metabolism
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Peng-Wei Yu, Peng-Fei Fu, Lei Zeng, Yan-Li Qi, Xiu-Qing Li, Qi Wang, Guo-Yu Yang, Hua-Wei Li, Jiang Wang, Bei-Bei Chu, and Meng-Di Wang
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autophagy ,epigallocatechin-3-gallate ,lipid synthesis ,porcine reproductive and respiratory syndrome virus ,Microbiology ,QR1-502 - Abstract
ABSTRACT Porcine reproductive and respiratory syndrome virus (PRRSV) infection leads to late-term reproductive failure and respiratory illness that affect the global swine industry. Epigallocatechin gallate (EGCG) is a polyphenolic compound from green tea that exerts antiviral activity against diverse viruses. This study aimed to report an uncharacterized mechanism of how EGCG restricted PRRSV proliferation. EGCG showed no significant effects on cell viability, cell cycle progression, and apoptosis in porcine alveolar macrophages and MARC-145 cells. The treatment of cells with EGCG attenuated the replication of both highly pathogenic and less pathogenic PRRSV in vitro. The viral life cycle analysis demonstrated that EGCG affected PRRSV replication and assembly, but not viral attachment, entry, or release. Interestingly, EGCG treatment abrogated the increased lipid droplets formation and lipid content induced by PRRSV infection. We further demonstrated that EGCG blocked PRRSV-stimulated expression of the key enzymes in lipid synthesis. In addition, EGCG attenuated PRRSV-induced autophagy that is critical for PRRSV proliferation. The supplementation of oleic acid restored PRRSV replication and assembly under EGCG treatment. Together, our results support that EGCG inhibits PRRSV proliferation through disturbing lipid metabolism. IMPORTANCE Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped single-positive-stranded RNA virus that causes acute respiratory distress in piglets and reproductive failure in sows, resulting in huge economic losses to the global swine industry. Several lines of evidence have suggested the crucial roles of lipids in PRRSV proliferation. Our previous report demonstrated that PRRSV activated lipophagy to facilitate viral replication through downregulating the expression of N-Myc downstream-regulated gene 1. The manipulation of lipid metabolism may be a new perspective to prevent PRRSV spread. In the present study, we reported that epigallocatechin-3-gallate (EGCG), the major component of green tea catechins, significantly attenuated PRRSV infection through inhibiting lipid synthesis and autophagy. Given that natural products derived from plants have helped in the prevention and treatment of various infectious diseases, EGCG has a great potential to serve as a safe and environmentally friendly natural compound to treat PRRSV infection.
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- 2022
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12. A {Co9}-Added Polyoxometalate for Efficient Visible-Light-Driven Hydrogen Evolution
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Zhen-Wen Wang and Guo-Yu Yang
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lacunary directing strategy ,polyoxometalates ,hydrothermal syntheses ,visible-light-driven hydrogen evolution ,Organic chemistry ,QD241-441 - Abstract
A polyanion cluster H6Na8Cs3[Co9(μ3-OH)3(H2O)6(HPO4)2(B-α-PW9O34)3]Cl·40H2O (1) was made with the guidance of the lacunary directing strategy under hydrothermal conditions. Compound 1 was characterized by single-crystal X-ray diffraction, powder X-ray diffraction, and thermogravimetric analysis, respectively. Single-crystal X-ray diffraction analyses showed that 1 consists of three anions [B-α-PW9O34]9− and a cyclic cationic [Co9(μ3-OH)3(H2O)6]15+ and two anions HPO42−. Variable-magnetic properties indicate antiferromagnetic interactions in 1. Visible-light-driven hydrogen evolution tests demonstrated that 1 was an efficient water reduction catalyst with an H2 evolution rate of 1217.6 μmol h−1 g−1.
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- 2023
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13. Two New Nonaborates with {B9} Cluster Open-Frameworks and Short Cutoff Edges
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Chong-An Chen and Guo-Yu Yang
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metal borates ,oxoboron cluster ,solvothermal syntheses ,short cutoff edges ,Organic chemistry ,QD241-441 - Abstract
Two new nonaborates, Na2Ba0.5[B9O15]·H2O (1) and Na4Ca1.5[B9O16(OH)2] (2), have been solvothermally made with mixed alkali and alkaline-earth metal cationic templates. 1 and 2 are constructed by two different types of nonaborate clusters. In 1, the [B9O19]11− cluster is composed of three corner-sharing [B3O7]5− clusters, of which two of them interconnect to the 1D B3O7-based chains and are further bridged to the 3D framework with 7 types of 10-MR channels by another [B3O7]5− bridging cluster. The [B9O18(OH)2]11− cluster in 2 is made of four BO4-sharing [B3O8]7− clusters. As a 4-connected node, the interconnections of [B9O18(OH)2]11− construct the unpreceded 2D layer with large 14-MR windows, which are further joined by H-bonds to the 3D supramolecular framework. UV–Vis absorption spectra reveal that both 1 and 2 have short cutoff edges below 190 nm, exhibiting bandgaps of 6.31 and 6.39 eV, respectively, indicating their potential applications in deep UV (DUV) regions.
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- 2022
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14. Designed Syntheses of Three {Ni6PW9}-Based Polyoxometalates, from Isolated Cluster to Cluster-Organic Helical Chain
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Chong-An Chen, Yan Liu, and Guo-Yu Yang
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polyoxometalates ,hydrothermal syntheses ,cluster-organic frameworks ,helical chain ,Organic chemistry ,QD241-441 - Abstract
Three new hexa-Ni-substituted Keggin-type polyoxometalates (POMs), [Ni6(OH)3- (DACH)3(H2O)6(PW9O34)]·31H2O (1), [Ni(DACH)2][Ni6(OH)3(DACH)3(HMIP)2(H2O)2(PW9O34)]·56 H2O (2), and [Ni(DACH)2][Ni6(OH)3(DACH)2(AP)(H2O)5(PW9O34)]·2H2O (3) (DACH = 1,2-Diami- nocyclohexane, MIP = 5-Methylisophthalate, AP = Adipate) were successfully made in the presence of DACH under hydrothermal conditions. 1 is an isolated hexa-Ni-substituted Keggin unit decorated by DACH. In order to further construct POM cluster-organic frameworks (POMCOFs) on the basis of 1, by analyzing the steric hindrances and orientations of the POM units, the rigid HMIP and flexible AP ligands were successively incorporated, and another anionic monomeric POM 2 and the new 1D POM cluster organic chain (POMCOC) 3 were obtained. HMIP ligand still acts as a decorating group on the Ni6 core of 2 but results in the different spatial arrangement of the {Ni6PW9} units. AP ligands in 3 successfully bridge adjacent isolated POM cluster units to 1D POMCOC with left-hand helices. The AP in 3 is the longest aliphatic carboxylic acid ligand in POMs, and the 1D POM cluster-AP helical chain represents the first 1D POMCOC with a helical feature.
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- 2022
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15. Myostatin knockout induces apoptosis in human cervical cancer cells via elevated reactive oxygen species generation
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Ying-Qian Han, Sheng-Li Ming, Hong-Tao Wu, Lei Zeng, Gen Ba, Jian Li, Wei-Fei Lu, Jie Han, Qia-Jun Du, Miao-Miao Sun, Guo-Yu Yang, Jiang Wang, and Bei-Bei Chu
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Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Myostatin (Mstn) is postulated to be a key determinant of muscle loss and cachexia in cancer. However, no experimental evidence supports a role for Mstn in cancer, particularly in regulating the survival and growth of cancer cells. In this study, we showed that the expression of Mstn was significantly increased in different tumor tissues and human cancer cells. Mstn knockdown inhibited the proliferation of cancer cells. A knockout (KO) of Mstn created by clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) 9 (CRISPR/Cas9) induced mitochondria-dependent apoptosis in HeLa cells. Furthermore, KO of Mstn reduced the lipid content. Molecular analyses demonstrated that the expression levels of fatty acid oxidation-related genes were upregulated and then increased rate of fatty acid oxidation. Mstn deficiency-induced apoptosis took place along with generation of reactive oxygen species (ROS) and elevated fatty acid oxidation, which may play a role in triggering mitochondrial membrane depolarization, the release of cytochrome c (Cyt-c), and caspase activation. Importantly, apoptosis induced by Mstn KO was partially rescued by antioxidants and etomoxir, thereby suggesting that the increased level of ROS was functionally involved in mediating apoptosis. Overall, our findings demonstrate a novel function of Mstn in regulating mitochondrial metabolism and apoptosis within cancer cells. Hence, inhibiting the production and function of Mstn may be an effective therapeutic intervention during cancer progression and muscle loss in cachexia. Keywords: Myostatin, CRISPR/Cas9, Apoptosis, Reactive oxygen species
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- 2018
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16. Pseudorabies Virus Inhibits Expression of Liver X Receptors to Assist Viral Infection
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Yi Wang, Guo-Li Li, Yan-Li Qi, Li-Yun Li, Lu-Fang Wang, Cong-Rong Wang, Xin-Rui Niu, Tao-Xue Liu, Jiang Wang, Guo-Yu Yang, Lei Zeng, and Bei-Bei Chu
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pseudorabies virus ,Liver X receptors ,clathrin-coated pits ,viral entry ,Microbiology ,QR1-502 - Abstract
Pseudorabies virus (PRV) is a contagious herpesvirus that causes Aujeszky’s disease and economic losses worldwide. Liver X receptors (LXRs) belong to the nuclear receptor superfamily and are critical for the control of lipid homeostasis. However, the role of LXR in PRV infection has not been fully established. In this study, we found that PRV infection downregulated the mRNA and protein levels of LXRα and LXRβ in vitro and in vivo. Furthermore, we discovered that LXR activation suppressed PRV proliferation, while LXR inhibition promoted PRV proliferation. We demonstrated that LXR activation-mediated reduction of cellular cholesterol was critical for the dynamics of PRV entry-dependent clathrin-coated pits. Replenishment of cholesterol restored the dynamics of clathrin-coated pits and PRV entry under LXR activation conditions. Interestingly, T0901317, an LXR agonist, prevented PRV infection in mice. Our results support a model that PRV modulates LXR-regulated cholesterol metabolism to facilitate viral proliferation.
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- 2022
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17. The Human-Specific STING Agonist G10 Activates Type I Interferon and the NLRP3 Inflammasome in Porcine Cells
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Sheng-Li Ming, Lei Zeng, Yu-Kun Guo, Shuang Zhang, Guo-Li Li, Ying-Xian Ma, Yun-Yun Zhai, Wen-Ru Chang, Le Yang, Jiang Wang, Guo-Yu Yang, and Bei-Bei Chu
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STING agonist ,NLRP3 inflammasome ,interferon ,negative regulation ,innate immunity ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Pigs have anatomical and physiological characteristics comparable to those in humans and, therefore, are a favorable model for immune function research. Interferons (IFNs) and inflammasomes have essential roles in the innate immune system. Here, we report that G10, a human-specific agonist of stimulator of interferon genes (STING), activates both type I IFN and the canonical NLRP3 inflammasome in a STING-dependent manner in porcine cells. Without a priming signal, G10 alone transcriptionally stimulated Sp1-dependent p65 expression, thus triggering activation of the nuclear factor-κB (NF-κB) signaling pathway and thereby priming inflammasome activation. G10 was also found to induce potassium efflux- and NLRP3/ASC/Caspase-1-dependent secretion of IL-1β and IL-18. Pharmacological and genetic inhibition of NLRP3 inflammasomes increased G10-induced type I IFN expression, thereby preventing virus infection, suggesting negative regulation of the NLRP3 inflammasome in the IFN response in the context of STING-mediated innate immune activation. Overall, our findings reveal a new mechanism through which G10 activates the NLRP3 inflammasome in porcine cells and provide new insights into STING-mediated innate immunity in pigs compared with humans.
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- 2020
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18. An effective inactivant based on singlet oxygen-mediated lipid oxidation implicates a new paradigm for broad-spectrum antivirals
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Lei Zeng, Meng-Di Wang, Sheng-Li Ming, Guo-Li Li, Peng-Wei Yu, Yan-Li Qi, Da-Wei Jiang, Guo-Yu Yang, Jiang Wang, and Bei-Bei Chu
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Singlet oxygen ,Lipid oxidation ,Membrane fusion ,Inactivated virus vaccine ,Immunization ,Broad-spectrum antivirals ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Emerging viral pathogens cause substantial morbidity and pose a severe threat to health worldwide. However, a universal antiviral strategy for producing safe and immunogenic inactivated vaccines is lacking. Here, we report an antiviral strategy using the novel singlet oxygen (1O2)-generating agent LJ002 to inactivate enveloped viruses and provide effective protection against viral infection. Our results demonstrated that LJ002 efficiently generated 1O2 in solution and living cells. Nevertheless, LJ002 exhibited no signs of acute toxicity in vitro or in vivo. The 1O2 produced by LJ002 oxidized lipids in the viral envelope and consequently destroyed the viral membrane structure, thus inhibiting the viral and cell membrane fusion necessary for infection. Moreover, the 1O2-based inactivated pseudorabies virus (PRV) vaccine had no effect on the content of the viral surface proteins. Immunization of mice with LJ002-inactiviated PRV vaccine harboring comparable antigen induced more neutralizing antibody responses and efficient protection against PRV infection than conventional formalin-inactivated vaccine. Additionally, LJ002 inactivated a broad spectrum of enveloped viruses. Together, our results may provide a new paradigm of using broad-spectrum, highly effective inactivants functioning through 1O2-mediated lipid oxidation for developing antivirals that target the viral membrane fusion process.
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- 2020
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19. BRD4 inhibition exerts anti-viral activity through DNA damage-dependent innate immune responses.
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Jiang Wang, Guo-Li Li, Sheng-Li Ming, Chun-Feng Wang, Li-Juan Shi, Bing-Qian Su, Hong-Tao Wu, Lei Zeng, Ying-Qian Han, Zhong-Hu Liu, Da-Wei Jiang, Yong-Kun Du, Xiang-Dong Li, Gai-Ping Zhang, Guo-Yu Yang, and Bei-Bei Chu
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Immunologic diseases. Allergy ,RC581-607 ,Biology (General) ,QH301-705.5 - Abstract
Chromatin dynamics regulated by epigenetic modification is crucial in genome stability and gene expression. Various epigenetic mechanisms have been identified in the pathogenesis of human diseases. Here, we examined the effects of ten epigenetic agents on pseudorabies virus (PRV) infection by using GFP-reporter assays. Inhibitors of bromodomain protein 4 (BRD4), which receives much more attention in cancer than viral infection, was found to exhibit substantial anti-viral activity against PRV as well as a range of DNA and RNA viruses. We further demonstrated that BRD4 inhibition boosted a robust innate immune response. BRD4 inhibition also de-compacted chromatin structure and induced the DNA damage response, thereby triggering the activation of cGAS-mediated innate immunity and increasing host resistance to viral infection both in vitro and in vivo. Mechanistically, the inhibitory effect of BRD4 inhibition on viral infection was mainly attributed to the attenuation of viral attachment. Our findings reveal a unique mechanism through which BRD4 inhibition restrains viral infection and points to its potent therapeutic value for viral infectious diseases.
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- 2020
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20. Novel 6a,12b-Dihydro-6H,7H-chromeno[3,4-c] chromen-6-ones: Synthesis, Structure and Antifungal Activity
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Jin-ping Bao, Cui-lian Xu, Guo-yu Yang, Cai-xia Wang, Xin Zheng, and Xin-xin Yuan
- Subjects
dihydrocoumarins ,synthesis ,3-trifluoroacetyl coumarins ,phenols ,antifungal activities ,Organic chemistry ,QD241-441 - Abstract
A new series of coumarin derivatives, 7-hydroxy-7-(trifluoromethyl)-6a,12b-dihydro-6H,7H-chromeno[3,4-c]chromen-6-ones 3a−p, were synthesized via Michael addition, transesterification and nucleophilic addition from the reaction of 3-trifluoroacetyl coumarins and phenols in the presence of an organic base. The products were characterized by infrared spectroscopy (IR), hydrogen nuclear magnetic resonance spectroscopy (1H-NMR), carbon nuclear magnetic resonance spectroscopy (13C-NMR) and high-resolution mass spectrometer (HRMS). Single crystal X-ray analysis of compounds 3a and 3n clearly confirmed their assigned chemical structures and their twisted conformations. Compound 3a crystallized in the orthorhombic system, Pbca, in which a = 8.6244(2) Å, b = 17.4245(4) Å, c = 22.5188(6) Å, α = 90°, β = 90°, γ = 90°, v = 3384.02(14) Å3, and z = 8. In addition, the mycelial growth rate method was used to examine the in vitro antifungal activities of the title compounds 3a−p against Fusarium graminearum and Fusarium monitiforme at 500 µg/mL. The results showed that compound 3l exhibited significant anti-Fusarium monitiforme activity with inhibitory index of 84.6%.
- Published
- 2019
- Full Text
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21. Cinnamyl 2-oxo-2H-chromene-3-carboxylate
- Author
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Cao-Yuan Niu, Su-Fang Fan, Guo-Yu Yang, Nan Yang, and Cui-Lian Xu
- Subjects
Crystallography ,QD901-999 - Abstract
The title compound, C19H14O4, was prepared by the reaction of 2-oxo-2H-chromene-3-acyl chloride with cinnamic alcohol. The whole molecule is not planar, the dihedral angle between the planes of coumarin and benzene rings being 13.94 (4)°, but the plane of the coumarin ring and that of the ester group are almost coplanar, making a dihedral angle of 2.9 (1)°. In the crystal structure, weak intermolecular C—H...O hydrogen bonds link two molecules into dimers, and π–π stacking interactions between inversion-related rings of the coumarin groups [centroid–centroid distance 3.8380 (15) Å with a slippage of 1.535 Å], which connect the dimers into columns extending along [010].
- Published
- 2009
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22. Menthyl 2-oxo-2H-chromene-3-carboxylate
- Author
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Cui-Lian Xu, Shan-Yu Liu, Gang Chen, Guo-Yu Yang, and Ming-Qin Zhao
- Subjects
Crystallography ,QD901-999 - Abstract
The title compound, C20H24O4, was synthesized from the reaction of 2-oxo-2H-chromene-3-acyl chloride and menthol. The mean plane of the ester group and that of the four essentially planar (maximum deviation 0.0112 Å) C atoms of the chair-form cyclohexyl ring form dihedral angles of 43.8 (3) ° and 81.8 (1)°, respectively, with the mean plane of the coumarin ring system. In the crystal structure, weak intermolecular C—H...O hydrogen bonds connect the molecules into a two-dimensional network.
- Published
- 2009
- Full Text
- View/download PDF
23. Covalent Organic Frameworks Meet Titanium Oxide.
- Author
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Rui Xue, Yinsheng Liu, Xueyan Wu, Yan Lv, Jixi Guo, and Guo-Yu Yang
- Published
- 2024
- Full Text
- View/download PDF
24. Pseudorabies virus manipulates mitochondrial tryptophanyl-tRNA synthetase 2 for viral replication.
- Author
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Xiu-Qing Li, Meng-Pan Cai, Ming-Yang Wang, Bo-Wen Shi, Guo-Yu Yang, Jiang Wang, Bei-Bei Chu, and Sheng-Li Ming
- Subjects
AUJESZKY'S disease virus ,RNA interference ,VIRAL replication ,TYPE I interferons ,GENE expression - Abstract
The pseudorabies virus (PRV) is identified as a double-helical DNA virus responsible for causing Aujeszky's disease, which results in considerable economic impacts globally. The enzyme tryptophanyl-tRNA synthetase 2 (WARS2), a mitochondrial protein involved in protein synthesis, is recognized for its broad expression and vital role in the translation process. The findings of our study showed an increase in both mRNA and protein levels of WARS2 following PRV infection in both cell cultures and animal models. Suppressing WARS2 expression via RNA interference in PK-15 cells led to a reduction in PRV infection rates, whereas enhancing WARS2 expression resulted in increased infection rates. Furthermore, the activation of WARS2 in response to PRV was found to be reliant on the cGAS/STING/TBK1/IRF3 signaling pathway and the interferon-alpha receptor-1, highlighting its regulation via the type I interferon signaling pathway. Further analysis revealed that reducing WARS2 levels hindered PRV's ability to promote protein and lipid synthesis. Our research provides novel evidence that WARS2 facilitates PRV infection through its management of protein and lipid levels, presenting new avenues for developing preventative and therapeutic measures against PRV infections. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
25. A novel strategy for amplified probing versatile biomolecules through a photoswitchable biocatalytic cascade
- Author
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Li, Ping, Cao, Gen-Xia, Liu, Qingyun, Guo, Yu-Yang, Dong, Yuming, Li, Zaijun, and Wang, Guang-Li
- Published
- 2018
- Full Text
- View/download PDF
26. Construction of a rAAV-SaCas9 system expressing eGFP and its application to improve muscle mass
- Author
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Shaoting Weng, Guo-Yu Yang, Jiang Wang, Yitian Zhao, Xiaofan Wang, Kunpeng Zhang, Xuehan Xiao, Changhong Yu, and Liqiang Han
- Subjects
Male ,viruses ,Genetic Vectors ,Green Fluorescent Proteins ,Bioengineering ,Myostatin ,Applied Microbiology and Biotechnology ,Green fluorescent protein ,Mice ,Genome editing ,In vivo ,medicine ,Animals ,Guide RNA ,Skeletal muscle proliferation ,Muscle, Skeletal ,Gene ,Gene Editing ,biology ,Promoter ,General Medicine ,Dependovirus ,Muscle atrophy ,Cell biology ,Original Research Paper ,eGFP protein ,Mice, Inbred C57BL ,rAAV-SaCas9 system ,biology.protein ,medicine.symptom ,CRISPR-Cas Systems ,Biotechnology - Abstract
An ideal rAAV gene editing system not only effectively edits genes at specific site, but also prevents the spread of the virus from occurring off-target or carcinogenic risks. This is important for gene editing research at specific site in vivo. We report a single rAAV containing SaCas9 and guide RNAs under the control of subtle EF1a and tRNA promoters. The capacity of rAAV was compressed, and the editing efficiency was similar to that of the classical Cas9 system in vitro and in vivo. And we inserted the sequence of the green fluorescent protein eGFP into rAAV. The number of cells infected with the rAAV and the region in which the rAAV spreads were known by the fluorescent expression of eGFP in cells. In addition, we demonstrated that myostatin gene in the thigh muscles of C57BL/10 mice was knocked out by the rAAV9-SaCas9 system to make muscle mass increased obviously. The protein eGFP into rAAV has significant implications for our indirect analysis of the editing efficiency of SaCas9 in the genome of the target tissue and reduces the harm caused by off-target editing and prevents other tissue mutations. The rAAV system has substantial potential in improving muscle mass and preventing muscle atrophy.
- Published
- 2021
27. Improvement of muscular atrophy by AAV–SaCas9-mediated myostatin gene editing in aged mice
- Author
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Xingyu Li, Bei-Bei Chu, Jiang Wang, Feng Gao, Shaoting Weng, Guo-Yu Yang, and Juan Wang
- Subjects
0301 basic medicine ,Cancer Research ,Aging ,Myostatin ,Biology ,MyoD ,Article ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Atrophy ,medicine ,Myocyte ,Animals ,Humans ,Molecular Biology ,Gene Editing ,Skeletal muscle ,medicine.disease ,musculoskeletal system ,Cell biology ,Gene regulation ,Muscular Atrophy ,030104 developmental biology ,medicine.anatomical_structure ,Sarcopenia ,Genetic engineering ,biology.protein ,Molecular Medicine ,PAX7 ,Signal transduction ,030217 neurology & neurosurgery - Abstract
Muscle mass and area usually decrease with age, and this phenomenon is known as sarcopenia. This age-related atrophy correlates with insufficient levels of muscle cells differentiate and proliferate regulated by the TGF-β signaling pathway and the expression of E3s ubiquitin-protein ligase by the aged. Sarcopenia makes a huge impact on the aging society, because it has the characteristic of high incidence, extensive adverse effects and disease aggravation gradually. Guided by a single-guide RNA (sgRNA), Cas9 nuclease has been widely used in genome editing, opening up a new pathway for sarcopenia treatment. Here, we present two rAAV9 systems, pX601-AAV-CMV:SaCas9-U6:sgRNA and pX601-AAV-EF1α:SaCas9-tRNAGLN: sgRNA, which edited myostatin efficiently. By delivering the two rAAV–SaCas9 targets to myostatin via intramuscular injection of aged mice, an increase in body weight and an increase in the number and area of myofibers were observed. Knockout of myostatin led to TGF-β signaling pathway changes, and increased MyoD, Pax7 and MyoG protein levels and increased the number of satellite cells to improve muscle cells differentiation. Moreover, knockout of myostatin prevented the atrophy of muscle cells through reduced Murf1 and MAFbx protein levels. We found that both rAAV–SaCas9 systems had gene editing efficiency, reducing the expression of myostatin by affecting the relevant signaling pathways, thereby altering the physiological status. We showed that myostatin has an important role in activating skeletal muscle proliferation and inhibiting muscular atrophy during aging. Thus, we propose that knockout of myostatin using the rAAV9–SaCas9 system has significant therapeutic potential in sarcopenia.
- Published
- 2020
28. Extracellular production of recombinant sus scrofa trefoil factor 3 by Brevibacillus choshinensis
- Author
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Guo-Yu Yang, Li Heping, Xiang‑Yang Jin, An‑Qi Li, Bing‑Yan Wen, Chun‑Mei Xu, Yun‑Ze Zhao, Kai Zhong, Lu‑Ping Feng, Ye‑Dong Cao, Guang‑Ming Zha, and Yue‑Ying Wang
- Subjects
0301 basic medicine ,Cancer Research ,Brevibacillus choshinensis ,medicine.disease_cause ,Microbiology ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Immunology and Microbiology (miscellaneous) ,Shuttle vector ,Downregulation and upregulation ,law ,Extracellular ,medicine ,Escherichia coli ,protein expression ,Brevibacillus ,biology ,Trefoil factor 3 ,trefoil factor 3 ,General Medicine ,Articles ,biology.organism_classification ,Blot ,030104 developmental biology ,pNCMO2 ,030220 oncology & carcinogenesis ,Recombinant DNA - Abstract
Trefoil factor 3 (TFF3) is involved in cell adhesion, motility and apoptosis, regulates mucosal immunity and maintains the functional integrity of intestinal epithelia. The upregulation of TFF3 expression in the weaning rat intestine attracted our interest. The present study hypothesized that TFF3 may serve a role in preventing diarrhea in weaning piglets, which is an important consideration in the pig farming industry. Previous recombinant TFF3 protein expression yields obtained from Escherichia coli were too low and the bioactivity of the protein was poor. Hence, this expression system was unsuitable for industrial applications. The present study explored the production of recombinant sus scrofa TFF3 in a Brevibacillus choshinensis (B. choshinensis) expression system, aiming to enhance the expression level of bioactive protein. To achieve this, the sus scrofa TFF3-encoding gene fragment was fused into an E. coli-Brevibacillus shuttle vector pNCMO2. High levels of TFF3 (30 mg/l) were produced and secreted into the B. choshinensis culture medium in soluble form with a molecular mass of 13.6 kDa and high immunoreactivity in western blotting. Thus, Brevibacillus may be used to produce useful mucosal factors for biochemical analyses and mucosal protection, and in industrial applications to produce novel inhibitors of diarrhea.
- Published
- 2020
29. The Human-Specific STING Agonist G10 Activates Type I Interferon and the NLRP3 Inflammasome in Porcine Cells
- Author
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Ying-Xian Ma, Lei Zeng, Bei-Bei Chu, Jiang Wang, Yun-Yun Zhai, Le Yang, Guo-Li Li, Shuang Zhang, Guo Yukun, Wen-Ru Chang, Sheng-Li Ming, and Guo-Yu Yang
- Subjects
0301 basic medicine ,lcsh:Immunologic diseases. Allergy ,Inflammasomes ,Sp1 Transcription Factor ,THP-1 Cells ,Sus scrofa ,Immunology ,Thiazines ,Priming (immunology) ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Interferon ,Chlorocebus aethiops ,NLR Family, Pyrin Domain-Containing 3 Protein ,STING agonist ,medicine ,Animals ,Humans ,Immunology and Allergy ,Secretion ,negative regulation ,Vero Cells ,innate immunity ,Original Research ,Innate immune system ,Caspase 1 ,Transcription Factor RelA ,Membrane Proteins ,Inflammasome ,interferon ,Immunity, Innate ,NLRP3 inflammasome ,Cell biology ,CARD Signaling Adaptor Proteins ,HEK293 Cells ,030104 developmental biology ,Stimulator of interferon genes ,Interferon Type I ,Signal transduction ,lcsh:RC581-607 ,Signal Transduction ,030215 immunology ,medicine.drug - Abstract
Pigs have anatomical and physiological characteristics comparable to those in humans and, therefore, are a favorable model for immune function research. Interferons (IFNs) and inflammasomes have essential roles in the innate immune system. Here, we report that G10, a human-specific agonist of stimulator of interferon genes (STING), activates both type I IFN and the canonical NLRP3 inflammasome in a STING-dependent manner in porcine cells. Without a priming signal, G10 alone transcriptionally stimulated Sp1-dependent p65 expression, thus triggering activation of the nuclear factor-κB (NF-κB) signaling pathway and thereby priming inflammasome activation. G10 was also found to induce potassium efflux- and NLRP3/ASC/Caspase-1-dependent secretion of IL-1β and IL-18. Pharmacological and genetic inhibition of NLRP3 inflammasomes increased G10-induced type I IFN expression, thereby preventing virus infection, suggesting negative regulation of the NLRP3 inflammasome in the IFN response in the context of STING-mediated innate immune activation. Overall, our findings reveal a new mechanism through which G10 activates the NLRP3 inflammasome in porcine cells and provide new insights into STING-mediated innate immunity in pigs compared with humans.
- Published
- 2020
30. An effective inactivant based on singlet oxygen-mediated lipid oxidation implicates a new paradigm for broad-spectrum antivirals
- Author
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Da-Wei Jiang, Guo-Yu Yang, Bei-Bei Chu, Jiang Wang, Peng-Wei Yu, Meng-Di Wang, Guo-Li Li, Sheng-Li Ming, Lei Zeng, and Yan-Li Qi
- Subjects
0301 basic medicine ,viruses ,Clinical Biochemistry ,Pseudorabies ,Membrane fusion ,Inactivated virus vaccine ,Biochemistry ,Antiviral Agents ,Virus ,Article ,Broad-spectrum antivirals ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Viral envelope ,Antigen ,Lipid oxidation ,Animals ,Neutralizing antibody ,lcsh:QH301-705.5 ,lcsh:R5-920 ,biology ,Chemistry ,Singlet oxygen ,Organic Chemistry ,Lipid bilayer fusion ,Viral membrane ,Virus Internalization ,biology.organism_classification ,Virology ,030104 developmental biology ,Vaccines, Inactivated ,lcsh:Biology (General) ,Virus Diseases ,biology.protein ,Immunization ,lcsh:Medicine (General) ,030217 neurology & neurosurgery - Abstract
Emerging viral pathogens cause substantial morbidity and pose a severe threat to health worldwide. However, a universal antiviral strategy for producing safe and immunogenic inactivated vaccines is lacking. Here, we report an antiviral strategy using the novel singlet oxygen (1O2)-generating agent LJ002 to inactivate enveloped viruses and provide effective protection against viral infection. Our results demonstrated that LJ002 efficiently generated 1O2 in solution and living cells. Nevertheless, LJ002 exhibited no signs of acute toxicity in vitro or in vivo. The 1O2 produced by LJ002 oxidized lipids in the viral envelope and consequently destroyed the viral membrane structure, thus inhibiting the viral and cell membrane fusion necessary for infection. Moreover, the 1O2-based inactivated pseudorabies virus (PRV) vaccine had no effect on the content of the viral surface proteins. Immunization of mice with LJ002-inactiviated PRV vaccine harboring comparable antigen induced more neutralizing antibody responses and efficient protection against PRV infection than conventional formalin-inactivated vaccine. Additionally, LJ002 inactivated a broad spectrum of enveloped viruses. Together, our results may provide a new paradigm of using broad-spectrum, highly effective inactivants functioning through 1O2-mediated lipid oxidation for developing antivirals that target the viral membrane fusion process., Highlights • LJ002 efficiently generates 1O2 in solution and living cells. • LJ002 oxidizes lipids in the viral envelope, thus inhibiting fusion between the virus and cell membrane. • LJ002-inactivated PRV vaccine has no effect on the content of antigens on the viral surface. • LJ002-inactivated PRV vaccine elicits a strong neutralizing antibody response. • LJ002 can inactivate a broad spectrum of enveloped viruses.
- Published
- 2020
31. BRD4 inhibition exerts anti-viral activity through DNA damage-dependent innate immune responses
- Author
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Hong-Tao Wu, Jiang Wang, Li-Juan Shi, Bing-Qian Su, Guo-Yu Yang, Gaiping Zhang, Sheng-Li Ming, Bei-Bei Chu, Ying-Qian Han, Chun-Feng Wang, Guo-Li Li, Dawei Jiang, Lei Zeng, Xiang-Dong Li, Zhong-Hu Liu, and Yong-Kun Du
- Subjects
Swine ,viruses ,Gene Expression ,Apoptosis ,Cell Cycle Proteins ,Biochemistry ,Madin Darby Canine Kidney Cells ,Histones ,Mice ,Spectrum Analysis Techniques ,RNA Virus Infections ,Gene expression ,Chlorocebus aethiops ,Medicine and Health Sciences ,Biology (General) ,0303 health sciences ,Innate Immune System ,Mice, Inbred BALB C ,Chromatin ,Viral transmission and infection ,Immunoblotting ,Flow cytometry ,Viral attachment ,Antiviral therapy ,Cell Death ,Chromosome Biology ,030302 biochemistry & molecular biology ,Nuclear Proteins ,Flow Cytometry ,DNA Virus Infections ,Cell biology ,Histone ,Cell Processes ,Spectrophotometry ,Epigenetics ,Female ,Cytophotometry ,Research Article ,DNA damage ,QH301-705.5 ,Immunology ,Molecular Probe Techniques ,Biology ,Research and Analysis Methods ,Microbiology ,Viral Attachment ,Virus ,03 medical and health sciences ,Dogs ,Immunity ,Virology ,DNA-binding proteins ,Genetics ,Animals ,Humans ,RNA Viruses ,Molecular Biology Techniques ,Molecular Biology ,Vero Cells ,030304 developmental biology ,Innate immune system ,DNA Viruses ,Biology and Life Sciences ,Proteins ,Cell Biology ,RC581-607 ,Immunity, Innate ,HEK293 Cells ,RAW 264.7 Cells ,A549 Cells ,Immune System ,biology.protein ,NIH 3T3 Cells ,Parasitology ,Immunologic diseases. Allergy ,Viral Transmission and Infection ,DNA Damage ,HeLa Cells ,Transcription Factors - Abstract
Chromatin dynamics regulated by epigenetic modification is crucial in genome stability and gene expression. Various epigenetic mechanisms have been identified in the pathogenesis of human diseases. Here, we examined the effects of ten epigenetic agents on pseudorabies virus (PRV) infection by using GFP-reporter assays. Inhibitors of bromodomain protein 4 (BRD4), which receives much more attention in cancer than viral infection, was found to exhibit substantial anti-viral activity against PRV as well as a range of DNA and RNA viruses. We further demonstrated that BRD4 inhibition boosted a robust innate immune response. BRD4 inhibition also de-compacted chromatin structure and induced the DNA damage response, thereby triggering the activation of cGAS-mediated innate immunity and increasing host resistance to viral infection both in vitro and in vivo. Mechanistically, the inhibitory effect of BRD4 inhibition on viral infection was mainly attributed to the attenuation of viral attachment. Our findings reveal a unique mechanism through which BRD4 inhibition restrains viral infection and points to its potent therapeutic value for viral infectious diseases., Author summary BRD4 has been well investigated in tumorigenesis for its contribution to chromatin remodeling and gene transcription. BRD4 inhibitors are used as promising chemotherapeutic drugs for cancer therapy. Here, we show a unique mechanism through which BRD4 inhibition broadly inhibits attachment of DNA and RNA viruses through DNA damage-dependent antiviral innate immune activation via the cGAS-STING pathway, in both cell culture and an animal model. STING-associated innate immune signaling has been considered to be a new possibility for cancer therapy, and STING agonists have been tested in early clinical trials. Our data identify BRD4 inhibitors as a potent therapy not only for viral infection but also for cancer immunotherapy.
- Published
- 2020
32. Bioinspired wood-like coaxial fibers based on MXene@graphene oxide with superior mechanical and electrical properties
- Author
-
Guo-Yu Yang, Xing Chen, Yujun Li, Jian-Jun Jiang, and Chuan-Bing Li
- Subjects
Materials science ,Graphene ,Oxide ,Nanotechnology ,Conductivity ,law.invention ,chemistry.chemical_compound ,chemistry ,law ,Ultimate tensile strength ,General Materials Science ,Fiber ,MXenes ,Nanoscopic scale ,Layer (electronics) - Abstract
MXenes, a new class of two-dimensional materials with excellent performance, are promising materials for wearable energy storage devices. However, the lack of sufficient interaction between various MXene particles makes it difficult to translate the exceptional performance from the nanoscale to macroscale. Additionally, the intrinsic characteristic of easy oxidation limits their practical applications. Herein, inspired by the structure of wood, a biomimetic core-shell MXene@graphene oxide (MX@GO) fiber was fabricated using GO as a mechanical layer to wrap MXenes. The GO layer could easily assemble MXene particles into macroscale materials and protect them from oxidation. Therefore, the as-fabricated core-shell MX@GO fiber showed a high tensile strength (290 MPa) and excellent electrical conductivity (2400 S m-1). Notably, the conductivity of the biomimetic fiber only decreased to 1800 S m-1 with a reduction of about 30% at 100 °C. This work paves the way to fabricate MXene-based fibers with freely designed functionalities and morphologies, which are suitable for various high-value fabric-based applications.
- Published
- 2020
33. Two organically templated niobium and zinconiobium fluorophosphates: Low temperature hydrothermal syntheses of NbOF[(P[O.sub.4]).sub.2][([C.sub.2][H.sub.10][N.sub.2]).sub.2] and [Zn.sub.3](NbOF)[(P[O.sub.4]).sub.4][([C.sub.2][H.sub.10][N.sub.2]).sub.2]
- Author
-
Guang-Zhen Liu, Shou-Tian Zheng, and Guo-Yu Yang
- Subjects
Niobium -- Chemical properties ,Phosphates -- Chemical properties ,Zinc compounds -- Chemical properties ,Fluorine compounds -- Chemical properties ,Chemistry - Abstract
The preparation of two new niobium and zinconiobium fluorophosphates, NbOF[(P[O.sub.4]).sub.2][([C.sub.2][H.sub.10][N.sub.2]).sub.2] and [Zn.sub.3](NbOF)(P[O.sub.4]).sub.4][([C.sub.2][H.sub.10][N.sub.2]).sub.2] (2) was carried out under hydrothermal conditions using ethylenediamine as a template. The topolocal relationships between the 4-connected network of (2) and several reported (3,4)-connected networks were discussed.
- Published
- 2007
34. {Ti6}/{Ti10} Wheel Cluster Substituted Silicotungstate Aggregates.
- Author
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Hai-Lou Li, Chen Lian, and Guo-Yu Yang
- Published
- 2021
- Full Text
- View/download PDF
35. LiB9O15·H2dap·H2O: A Cotemplated Acentric Layer-Pillared Borate Built by Mixed Oxoboron Clusters.
- Author
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Xu-Yan Li and Guo-Yu Yang
- Published
- 2021
- Full Text
- View/download PDF
36. A {Ti6W4}-Cluster-Substituted Polyoxotungstate: Synthesis, Structure, and Catalytic Oxidation Properties.
- Author
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Hai-Lou Li, Chen Lian, and Guo-Yu Yang
- Published
- 2021
- Full Text
- View/download PDF
37. Synthesis of Two New Ni12-Cluster Substituted Sandwiched Phosphotungstates Organic-Cluster and their Magnetic Property.
- Author
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Zhao-Min Su, Qingqing An, Xiaomei Xu, Niu Zhang, Lixia Bao, Zhiyu Jia, and Guo-Yu Yang
- Subjects
MAGNETIC properties ,X-ray crystallography ,POLYOXOMETALATES ,HYDROTHERMAL synthesis ,NAPHTHALENE - Abstract
Two new Ni12-substituted sandwiched phosphotungstates organic-cluster, H
2 [Ni6 (OH)3(H2 O)9 (PW9 O34 )(1,4-NDC)1/2 (H2 O)1/2 ]2 ·14 H2 O (1), Ni(H2 O)2(en)2[Ni6 (OH)3(H2 O)6(en)2(PW9 O34 )(2,6-NDC)1/2 ]2 ·11H2 O (2) (en=1,2-ethylenediamine; 1,4-NDC=Naphthalene-1,4-dicarboxylic acid; 2,6-NDC=Naphthalene-2,6-dicarboxylic acid) which are composed of {Ni6 PW9 } as second building units (SBU) and organic molecular as the bridging molecule have been synthesized by a hydrothermal method under mild condition. From single-crystal X-ray crystallography, we could observe two different configurations between 1 and 2. Two {Ni6 PW9 } SBUs were connected through 1,4-H2 NDC molecular and 2,6-H2 NDC molecular, respectively. Thought the connection of organic molecular, we successfully increase the nuclear number of transition-metal-substituted polyoxometalates. Meanwhile, both of two compounds have ferromagnetic interactions, which guides us to synthesize novel phosphotungstates with ferromagnetic property. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
38. Hydrothermal synthesis and characterization of (enH 2) 3.5[As 8V 14O 42(PO 4)]·2H 2O
- Author
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Cui, Xiao-Bing, Xu, Ji-Qing, Li, Yong, Sun, Ying-Hua, Ye, Ling, and Guo-Yu, Yang
- Published
- 2003
- Full Text
- View/download PDF
39. Photoluminescent Metallaprisms with (p-Cymene)Ru-Corners and Bis(β-diketone) Pillars.
- Author
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Ming-Jie Yan, Sheng-Li Huang, and Guo-Yu Yang
- Subjects
PHOTOLUMINESCENCE ,ETHYLENE ,ALKENES ,SOLVENTS ,LUMINESCENCE ,RUTHENIUM compounds ,PHOTOLUMINESCENT polymers - Abstract
Four metallaprisms were synthesized by the selective combination of (p-cymene)Ru-corners, bis(β-diketone) pillars, and multifunctional pyridyl linkers. They have large cavities and exhibit photoluminescence in CH
2 Cl2 solvent, particularly the employment of AIE-active ligand tetra-(4-pyridylphenyl)ethylene (TPPE) endows one of them with AIE properties. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
40. Combination of lacunary polyoxometalates and high-nuclear transition-metal clusters under hydrothermal conditions. 5. A novel tetrameric cluster of [[{[Fe.sup.II][Fe.sub.12.sup.II][([[mu].sub.3]-OH).sub.12][([[mu].sub.4]P[O.sub.4]).sub.4]}[(B-[alpha]-P[W.sub.9][O.sub.34]).sub.4]].sup.22-]
- Author
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Jun-Wei Zhao, Jie Zhang, Shou-Tian Zheng, and Guo-Yu Yang
- Subjects
Iron compounds -- Research ,Iron compounds -- Chemical properties ,Coordination compounds -- Research ,Coordination compounds -- Chemical properties ,Chemistry - Abstract
A novel tertrameric small lacunary polyoxometalate (POM) cluster is synthesized by a combination of mild hydrothermal conditions with trivacant Keggin POM precursors. The synthesis has provided a possible method for constructing the high-nuclear poly(POM) clusters.
- Published
- 2007
41. K2[Ge(B4)9)].2H2O: A unique 3D alternating linkage mode of a B4O9 cluster and GeO4 unit in Borogermanate with two pairs of interweaving double helical channels
- Author
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Hong-Xia Zhang, Jie Zhang, Shou-Tian Zheng, Guo-Ming Wang, and Guo-Yu Yang
- Subjects
Germanium -- Chemical properties ,Potassium compounds -- Chemical properties ,Chemistry - Abstract
A novel borogermanate of K2Ge(B4O9).2H2O with 3D structure constructed from B4O9 cluster and GeO4 unit was synthesized under solvothermal conditions. The structures possess two pairs of interweaving double helical channels with 10-membered rings and exhibits distinct NLO properties because it lacks a center of symmetry.
- Published
- 2004
42. Structural variability in Neptunium(V) oxalate compounds: Synthesis and structural characterization of Na2NpO2(C2)4)OH.H2O
- Author
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Amanda C. Bean, Jie Zhang, Shou-Tian Zheng, Guo-Ming Wang, and Guo-Yu Yang
- Subjects
Oxalates -- Structure ,Hydrothermal deposits -- Analysis ,Neptunium -- Chemical properties ,Chemistry - Abstract
The preparation of single crystals of Na2NpO2(C2)4))H.H2O by hydrothermal synthesis is detailed. This compound is the first one, which contains hydroxide in the Np(V)-oxalate system, demonstrating the structural variation that Np(V) can adopt.
- Published
- 2004
43. Metal-Organogermanate frameworks built by two kinds of infinite Ge-O chains with high thermostability and luminescent properties
- Author
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Gao-Juan Cao, Shou-Tian Zheng, Na Zhao, Jian-Ke Sun, and Guo-Yu Yang
- Subjects
Luminescence -- Analysis ,Germanium -- Electric properties ,Oxides -- Electric properties ,Chemistry - Published
- 2010
44. Cubic polyoxometalate-organic molecular cage
- Author
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Shou-Tian Zheng, Jie Zhang, Xin-Xiong Li, Wei-Hui Fang, and Guo-Yu Yang
- Subjects
Benzene -- Structure ,Benzene -- Chemical properties ,Methane -- Chemical properties ,Organometallic compounds -- Structure ,Organometallic compounds -- Chemical properties ,Organometallic compounds -- Thermal properties ,Substitution reactions -- Analysis ,Tungsten -- Chemical properties ,Chemistry - Abstract
Tris(hydroxymethyl)aminomethane (Tris) is grafted onto the surface of a [Ni.sub.6]-substituted polyoxotungstate formed in situ to generate a three-connected polyoxometalate building block. The cooperative assembly of Tris functionalized three-connected building blocks and rigid 1,3,5-benzenetricarboxylate has provided an unprecedented cubic polyoxometalate-organic molecular cage with high thermal and hydrothermal stability.
- Published
- 2010
45. Immobilization of Polyoxometalate in the Metal-Organic Framework rht-MOF-1: Towards a Highly Effective Heterogeneous Catalyst and Dye Scavenger
- Author
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Jing-Quan Sha, Pengfei Yan, Guang-Ming Li, Jing-Wen Sun, Guo-Yu Yang, and Guanghui An
- Subjects
Multidisciplinary ,Aqueous solution ,Chemistry ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Heterogeneous catalysis ,01 natural sciences ,Article ,0104 chemical sciences ,Catalysis ,Polyoxometalate ,Polymer chemistry ,Metal-organic framework ,Reactivity (chemistry) ,Copper chloride ,0210 nano-technology ,Selectivity - Abstract
A series of three remarkable complexes [PMo12O40]@[Cu6O(TZI)3(H2O)9]4·OH·31H2O (H3TZI = 5-tetrazolylisophthalic acid; denoted as HLJU-1, HLJU = Heilongjiang University), [SiMo12O40]@[Cu6O(TZI)3(H2O)9]4·32H2O (denoted as HLJU-2) and [PW12O40]@[Cu6O(TZI)3(H2O)6]4·OH·31H2O (denoted as HLJU-3) have been isolated by using simple one-step solvothermal reaction of copper chloride, 5-tetrazolylisophthalic acid (H3TZI) and various Keggin-type polyoxometalates (POMs), respectively. Crystal analysis of HLJU 1−3 reveals that Keggin-type polyoxoanions have been fitted snuggly in the cages of rht-MOF-1 (MOF: metal−organic framework) with large cell volume in a range of 87968−88800 Å3 and large pore volume of about 68%. HLJU 1–3 exhibit unique catalytic selectivity and reactivity in the oxidation of alkylbenzene with environmental benign oxidant under mild condition in aqueous phase as well as the uptake capacity towards organic pollutants in aqueous solution.
- Published
- 2016
- Full Text
- View/download PDF
46. Lanthanide germanate cluster organic frameworks constructed from {[Ln.sub.8][Ge.sub.12]} or {[Ln.sub.11][Ge.sub.12]} cage cluster building blocks
- Author
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Huan He, Gao-Juan Cao, Shou-Tian Zheng, and Guo-Yu Yang
- Subjects
Germanium -- Chemical properties ,Germanium -- Thermal properties ,Neodymium -- Chemical properties ,Organometallic compounds -- Structure ,Organometallic compounds -- Chemical properties ,Organometallic compounds -- Thermal properties ,Rare earth metals -- Chemical properties ,Rare earth metals -- Thermal properties ,Chemistry - Abstract
Bis(carboxyethylgermanium) sesquioxide is used as the germanium source for introducing lanthanide into germanate systems under hydrothermal conditions, resulting in a novel lanthanide germanate cluster organic framework with twofold-interpenetrating nets built from [Nd.sub.8][Ge.sub.12] cluster units. The interpenetration is avoided by introducing a second ligand, 2-picolinic acid, into the above system, resulting in the formation of a series of noninterpenetrating networks constructed from [Ln.sub.11][Ge.sub.12] cluster units.
- Published
- 2009
47. Combination chemistry of hexa-copper-substituted polyoxometalates driven by the [Cu.sup.II]-polyhedra distortion: from tetramer, 1D chain to 3D framework
- Author
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Bing Li, Jun-Wei Zhao, Shou-Tian Zheng, and Guo-Yu Yang
- Subjects
Antiferromagnetism -- Analysis ,Copper compounds -- Chemical properties ,Copper compounds -- Structure ,Ethylene -- Chemical properties ,Ethylene -- Structure ,Polyhedra -- Chemical properties ,Polyhedra -- Structure ,Chemistry - Published
- 2009
48. Aluminoborates with open frameworks: syntheses, structures, and properties
- Author
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Cheng Rong, Zhiwu Yu, Qiang Wang, Shou-Tian Zheng, Chun-Yang Pan, Feng Deng, and Guo-Yu Yang
- Subjects
Aluminum compounds -- Chemical properties ,Aluminum compounds -- Structure ,Fluorescence spectroscopy -- Usage ,Nuclear magnetic resonance -- Usage ,Tetrahedra -- Chemical properties ,Tetrahedra -- Structure ,X-rays -- Diffraction ,X-rays -- Usage ,Chemistry - Published
- 2009
49. Incorporating distinct metal clusters to construct diversity of 3D pillared-layer lanthanide-transition-metal frameworks
- Author
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Jian-Wen Cheng, Shou-Tian Zheng, and Guo-Yu Yang
- Subjects
Infrared spectroscopy -- Usage ,Chemistry - Abstract
Different metal clusters as building blocks under hydrothermal conditions were used to obtain three novel three-dimensional pillared-layer heterometallic lanthanide-transition-metal (hetero-Ln-TM) compounds. The compounds represent good examples of using metal clusters to construct fascinating three-dimensional hetero-Ln-TM frameworks.
- Published
- 2008
50. {[[Ln. sup.III][mu5-[K.sup.2],[K.sup.1],[K.sup.1],[K.sup.1],[K.sup.1],-1,2-(C[O.sub.2]).sub.2][C.sub.6][H.sub.4]][isonicotine][[H.sub.2]O]].sub.2}[Cu.sup1].X (Ln = Eu, Sm, Nd; X = CI[O.sub.4.sup-], [Cl.sup.-]: A new pillared-layer approach to heterobimetallic 3d-4f 3D-network solids
- Author
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Jian-Wen Cheng, Shou-Tian Zheng, En Ma, and Guo-Yu Yang
- Subjects
Infrared spectroscopy -- Usage ,Thermal analysis -- Usage ,Copper compounds -- Structure ,Copper compounds -- Thermal properties ,Copper compounds -- Optical properties ,Chemistry - Abstract
The pillared-layer open three dimensional 4f framework of the hydrothermally synthesized heterobimetallic lanthanide-copper coordination polymers is studied. The optical and luminescent properties of these complexes are investigated using IR spectroscopy.
- Published
- 2007
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