44 results on '"Huang, Hui-Chen"'
Search Results
2. Effect of Head-of-Bed Elevation on Nocturnal Reflux Symptoms of Esophageal Cancer Patients With Esophagectomy and Reconstruction
- Author
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Huang, Hui-Chen, Chang, Ying-Ju, Tseng, Yau-Lin, and Fang, Su-Ying
- Published
- 2021
- Full Text
- View/download PDF
3. Effect of Head-of-Bed Elevation on Nocturnal Reflux Symptoms of Esophageal Cancer Patients With Esophagectomy and Reconstruction
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Huang, Hui-Chen, Chang, Ying-Ju, Tseng, Yau-Lin, and Fang, Su-Ying
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- 2019
- Full Text
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4. Complication reports for robotic surgery using three arms by a single surgeon at a single institution
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Ching-Hui Chen, Huang-Hui Chen, and Wei-Min Liu
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Da Vinci surgical system ,perioperative complications ,robotic-assisted surgery ,Surgery ,RD1-811 ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background: The aim of this study is to evaluate perioperative complications related to robotic-assisted laparoscopic surgery for management of gynaecologic disorders. Materials and Methods: Eight hundred and fifty-one women who underwent robotic procedures between December 2011 and April 2015 were retrospectively included for analysis. Patient demographics, surgical outcomes and complications were evaluated. Results: The overall complication rate was 5.5%, whereas the rate of complications for oncologic cases was 8.4%. Intra-operative complications (n = 7, 0.8%) consisted of five cases of bowel lacerations, one case of ureter laceration and one case of bladder injury. Early and late post-operative complications were 4.0% (n = 34) and 0.8% (n = 6), respectively. Six patients (0.7%) experienced Grade III complications based on the Clavien-Dindo classification and required further surgical intervention. Conclusion: Robotic-assisted laparoscopic surgery is a feasible approach for management of gynaecologic disorders; the complication rates for this type of procedure are acceptable.
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- 2017
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5. Delayed postcoital vaginal cuff dehiscence with small bowel evisceration after robotic-assisted staging surgery
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Yen-Po Lan, Huang-Hui Chen, Wei-Min Liu, and Ching-Hui Chen
- Subjects
chemotherapy ,postcoital vaginal evisceration ,robotic surgery ,vaginal cuff dehiscence ,Gynecology and obstetrics ,RG1-991 - Abstract
Objective: We report a rare case of vaginal cuff dehiscence with small bowel evisceration at 7 months post robotic-staging surgery. Case Report: A 41-year-old woman was sent to the emergency room with sudden onset of abdominal pain, vaginal bleeding, and vaginal protruding mass after sexual activity. She had a history of synchronous uterine and ovarian cancer treated with robotic-staging surgery 7 months before. Then she received six courses of postoperative adjuvant chemotherapy, and the last chemotherapy ended 1 month ago. At the operation room, some small bowel loops were noted in the vaginal tip with cuff dehiscence and bleeding. After repositioning of the small bowel, a 2.5-cm vaginal cuff dehiscence was repaired transvaginally. The patient recovered well, and is free of disease and has normal sexual activity 2 months after repairs. Conclusion: Unusual delayed-type vaginal cuff dehiscence hints the possibility that a combination of robotic surgery and postoperative chemotherapy might result in delayed healing of the vaginal cuff.
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- 2017
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6. Pills-related severe adverse events: A case report in Taiwan
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Ching-Hui Chen, Hung-Yen Chin, Huang-Hui Chen, Heng-Yu Chang, and Wei-Min Liu
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abnormal uterine bleeding ,Diane-35 ,polycystic ovary syndrome ,pulmonary embolism ,thromboembolism ,Gynecology and obstetrics ,RG1-991 - Abstract
Objective: To review and evaluate the potential adverse effects of these oral contraceptives (OCP) to overweight women. Case Report: A 19-year-old college student, with a body mass index (BMI) of 35.2 kg/m2, who received 2 months of OCP containing cyproterone and ethinyl estradiol for polycystic ovary syndrome (PCOS)-related menstrual problems was complicated with a thromboembolism-related life-threatened disease. After intensive care, including the use of an extracorporeal membrane oxygenation system, thrombolytic treatment, anticoagulant, and inferior vena filter, she recovered well without significant sequelae. Conclusion: This case illustrates the risk of using OCPs, especially for those containing cyproterone and ethinyl estradiol components, as a treatment for menstrual problems in young women with PCOS and a high BMI.
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- 2016
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7. Elevated Urinary Hepcidin Level and Hypoferremia in Infants with Febrile Urinary Tract Infection: A Prospective Cohort Study.
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Hsu, Yu-Chen, Huang, Hsin-Chun, Tang, Kuo-Su, Su, Li-Ting, Huang, Ying-Hsien, Huang, Hui-Chen, and Chen, I-Lun
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ANTIBIOTICS ,BIOMARKERS ,KRUSKAL-Wallis Test ,STATISTICS ,FEVER ,NEONATAL anemia ,URINARY tract infections ,CHILDREN'S hospitals ,MULTIPLE regression analysis ,CASE-control method ,FISHER exact test ,DYNAMICS ,T-test (Statistics) ,ESCHERICHIA coli diseases ,CHI-squared test ,DESCRIPTIVE statistics ,RESEARCH funding ,IRON deficiency anemia ,IRON regulatory proteins ,URINALYSIS ,ODDS ratio ,LOGISTIC regression analysis ,RECEIVER operating characteristic curves ,DATA analysis ,DATA analysis software ,LONGITUDINAL method ,CREATININE ,CHILDREN - Abstract
To evaluate the kinetics of serum and urinary hepcidin levels along with anemia-related parameters during the infection course of infants with febrile urinary tract infection (UTI), we enrolled febrile infants aged one to four months in this prospective study. Febrile patients with UTI were allocated into Escherichia coli (E. coli) or non-E. coli groups according to urine culture results. Septic workup, blood hepcidin, iron profile, urinalysis, and urinary hepcidin–creatinine ratio were collected upon admission and 3 days after antibiotic treatment. In total, 118 infants were included. On admission, the febrile UTI group showed a significant reduction in serum iron level and a significant elevation of urinary hepcidin–creatinine ratio compared to the febrile control counterpart. Moreover, urinary hepcidin–creatinine ratio had the highest odds ratio, 2.01, in logistics regression analysis. After 3 days of antibiotic treatment, hemoglobin and the urinary hepcidin–creatinine ratio were significantly decreased. Patients with an E. coli UTI had a significantly decreased urinary hepcidin–creatinine ratio after 3 days of antibiotics treatment, whereas the non-E. coli group showed insignificant changes. Our study suggested that the urinary hepcidin–creatinine ratio elevated during acute febrile urinary tract infection and significantly decreased after 3 days of antibiotics treatment, especially in E. coli UTI. [ABSTRACT FROM AUTHOR]
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- 2023
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8. Adlay (薏苡 yì yĭ; 'soft-shelled job's tears'; the seeds of Coix lachryma-jobi L. var. ma-yuen Stapf) is a Potential Cancer Chemopreventive Agent toward Multistage Carcinogenesis Processes
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Ching-Chuan Kuo, Ph.D., Huang-Hui Chen, and Wenchang Chiang, Ph.D.
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Adlay ,Traditional Chinese medicine ,Cancer chemoprevention ,Blocking agent ,Suppressing agent ,Medicine - Abstract
Adlay (薏苡 yì yĭ; “soft-shelled job’s tears”, the seeds of Coix lachryma-jobi L. var. ma-yuen Stapf) is a grass crop that has long been used in traditional Chinese medicine (TCM) and as a nourishing food in China for the treatment of warts, chapped skin, rheumatism, neuralgia, inflammatory, and neoplastic diseases. In addition, adlay also has been said to have stomachic, diuretic, antipholgistic, anodynic, and antispasmodic effects. Carcinogenesis is a multistage process that begins with exposure of viruses or chemicals that are found in the environment. Chemoprevention refers to the use of natural or synthetic, non-toxic chemical substances to reverse, repress, or prevent carcinogenesis. In this review, we summarize recent research attempting to study the chemopreventive blocking and suppressing potential of adlay and its active components in scavenging electrophiles and reactive oxygen species, antimutagenicity, enhancing Nrf2-mediated detoxification and antioxidant effect, altering carcinogen metabolism, suppressing proliferation, decreasing inflammation, and enhancing antitumor immunity. In addition, several active components with diverse chemopreventive properties have been also mentioned in this review article.
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- 2012
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9. Temperature-dependent ultra-thin polymer layer for low voltage organic thin-film transistors
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Huang, Ting-Hsiang, Huang, Hui-Chen, and Pei, Zingway
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- 2010
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10. Volatilization characteristics of organic compounds in the coagulation process
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Chao, Huan-Ping, Lee, Jiunn-Fwu, Lee, Chung-Kung, and Huang, Hui-Chen
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Polymers -- Environmental aspects ,Volatile organic compounds -- Research ,Environmental engineering -- Research ,Engineering and manufacturing industries ,Environmental issues - Abstract
The emissions of two types of organic solutes during the coagulation process were simulated using Jar Test equipment and two additives, coagulant and polymer, to evaluate the volatilization characteristics under various operating conditions. The solute volatilization rates were found to be a function of the liquid mixing intensity, the chemical properties of the additives, and the properties of solutes, including molecular weight (M), Henry's law constant (H), and water solubility (S). The volatilization rates of the high H solutes increased sharply as the mixing intensity increased. Moreover, the volatilization rates of selected compounds were only slightly dependent on the coagulant concentrations due to the inorganic property of the coagulant. On the other hand, the effective volatilization reduction of the high H solutes in the existence of organic flocculant was a result of the enhancement of solutes solubility in water solutions. However, the above inhibition effects decreased significantly when the mixing intensity increased. Finally, the emission rates of the low H solutes were weakly correlated with both the solution properties and the operating parameters, due to their high affinity with the solution and the major volatilization resistance existing in the gas phase. Two different approaches, i.e., the two-film theory and the modified Knudsen diffusion equation, were used to explain the solute volatilization characteristics in the simulation process. CE Database subject headings: Coagulation; Volatile organic chemicals; Volatilization; Polymers; Emissions; Organic compounds.
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- 2005
11. The structural and sorptive characteristics of high-surface-area carbonaceous material (HSACM) in soils
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Huang, Hui-Chen, Lee, Jiunn-Fwu, Lee, Chung-Kung, and Chen, Jiunn-Chen
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- 2006
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12. Organic compound distribution between nonionic surfactant solution and natural solids: Applicability of a solution property parameter
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Lee, Jiunn-Fwu, Chang, Yi-Tang, Chao, Huan-Ping, Huang, Hui-Chen, and Hsu, Ming-Hung
- Published
- 2006
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13. The influences of solid-phase organic constituents on the partition of aliphatic and aromatic organic contaminants
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Huang, Hui-Chen, Lee, Jiunn-Fwu, Chao, Huan-Ping, Yeh, Pei-Wen, Yang, Yi-Fan, and Liao, Wan-Li
- Published
- 2005
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14. Secreted Frizzled-Related Protein 1 Modulates Glucocorticoid Attenuation of Osteogenic Activities and Bone Mass
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Wang, Feng-Sheng, Lin, Chun-Liang, Chen, Yeung-Jen, Wang, Ching-Jen, Yang, Kuender D., Huang, Yu-Ting, Sun, Yi-Chih, and Huang, Hui-Chen
- Published
- 2005
15. Suppressed TGF-β1 Expression Is Correlated With Up-Regulation of Matrix Metalloproteinase-13 in Keloid Regression After Flashlamp Pulsed-Dye Laser Treatment
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Kuo, Yur-Ren, Wu, Wen-Shan, Jeng, Seng-Feng, Wang, Feng-Sheng, Huang, Hui-Chen, Lin, Cha-Zon, and Yang, Kuender D.
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- 2005
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16. Activation of ERK and p38 Kinase Mediated Keloid Fibroblast Apoptosis After Flashlamp Pulsed-Dye Laser Treatment
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Kuo, Yur-Ren, Wu, Wen-Sheng, Jeng, Seng-Feng, Huang, Hui-Chen, Yang, Kuender D., Sacks, Justin M., and Wang, Feng-Sheng
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- 2005
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17. The effect of surfactants on the distribution of organic compounds in the soil solid/water system
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Lee, Jiunn-Fwu, Hsu, Ming-Hung, Chao, Huan-Ping, Huang, Hui-Chen, and Wang, Shun-Ping
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- 2004
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18. Nitrosoglutathione modulation of platelet activation and nitric oxide synthase expression in promotion of flap survival after ischemia/reperfusion injury 1
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Kuo, Yur-Ren, Wang, Feng-Sheng, Jeng, Seng-Feng, Huang, Hui-Chen, Wei, Fu-Chan, and Yang, Kuender D
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- 2004
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19. c-MYC-directed NRF2 drives malignant progression of head and neck cancer via glucose-6-phosphate dehydrogenase and transketolase activation.
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Ya-Chu Tang, Jenn-Ren Hsiao, Shih-Sheng Jiang, Jang-Yang Chang, Pei-Yi Chu, Ko-Jiunn Liu, Hsun-Lang Fang, Li-Mei Lin, Huang-Hui Chen, Yen-Wen Huang, Yu-Tsen Chen, Fang-Yu Tsai, Su-Fang Lin, Yung-Jen Chuang, and Ching-Chuan Kuo
- Published
- 2021
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20. Nitric Oxide Donor Increases Osteoprotegerin Production and Osteoclastogenesis Inhibitory Activity in Bone Marrow Stromal Cells from Ovariectomized Rats
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Wang, Feng-Sheng, Wang, Ching-Jen, Chen, Yeung-Jen, Huang, Yu-Ting, Huang, Hui-Chen, Chang, Per-Rong, Sun, Yi-Chih, and Yang, Kuender D.
- Published
- 2004
21. Nitrosoglutathione improves blood perfusion and flap survival by suppressing iNOS but protecting eNOS expression in the flap vessels after ischemia/reperfusion injury
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Kuo, Yur-Ren, Wang, Feng-Sheng, Jeng, Seng-Feng, Lutz, Barbara S., Huang, Hui-Chen, and Yang, Kuender D.
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- 2004
22. Clinical Outcome of an All Arthroscopic ‘Whole Layer’ Rotator Cuff Repair Technique with Simultaneous Biceps Tenodesis.
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Shih, Chiu‐Yu, Lin, Pei‐Ru, Huang, Hui‐Chen, and Hsieh, Cheng‐Pu
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TENODESIS , *ROTATOR cuff , *SUBACROMIAL impingement syndrome , *PATIENT satisfaction , *ARTHROSCOPY , *BODY mass index - Abstract
Objective Methods Results Conclusion To retrospectively evaluate clinical outcomes, including function and pain, of patients after our all arthroscopic “whole layer” rotator cuff repair technique with simultaneous biceps tenodesis procedure; factors influencing results were also evaluated. Given the frequent association of rotator cuff tear with long head of biceps lesion and the need for effective combined treatment strategies, this study aims to evaluate the efficacy of our technique and compare it with established methods. We hypothesized that our technique would significantly improve function and reduce pain in patients with rotator cuff tears and biceps pathology.This is a retrospective study that included patients older than 20 years who underwent all arthroscopic “whole layer” rotator cuff repair technique with simultaneous biceps tenodesis procedures for concomitant rotator cuff tear and long head biceps pathology, from 2016 to 2020. Patients were evaluated preoperatively and at a minimum of 2 years of follow‐up using the American Shoulder and Elbow Surgeons (ASES) and visual analogue scale (VAS) scores paired t‐tests were used for analysis and statistical significance was set at p < 0.05(two‐tailed). The satisfaction rate and complications were also evaluated.After an average follow‐up of 2.3 years, 118 patients demonstrated significant improvement in both the ASES score (from 36.13 to 95.01, p < 0.001) and VAS score (from 6.81 to 0.89, p < 0.001). Ninety‐four percent of the patients reported satisfaction with the surgical outcome. No complications related to Popeye deformity, biceps cramping pain, or ipsilateral shoulder reoperation were observed. Factors such as age, sex, body mass index (BMI), smoking status, alcohol consumption, hypertension, and diabetes did not influence the results. Patients showed significant improvement in both the ASES and VAS scores (p < 0.001).At a mean follow‐up time of 2.3 years, the all arthroscopic “whole layer” rotator cuff repair technique with simultaneous biceps tenodesis is a therapeutic and efficient procedure. The procedure revealed a satisfactory functional outcome, reduced pain, and minimal complications and reoperations. [ABSTRACT FROM AUTHOR]
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- 2024
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23. Delayed postcoital vaginal cuff dehiscence with small bowel evisceration after robotic-assisted staging surgery
- Author
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Ching Hui Chen, Wei Min Liu, Huang Hui Chen, and Yen Po Lan
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Adult ,medicine.medical_specialty ,Abdominal pain ,Time Factors ,Ovariectomy ,medicine.medical_treatment ,Dehiscence ,Hysterectomy ,chemotherapy ,lcsh:Gynecology and obstetrics ,Salpingectomy ,03 medical and health sciences ,0302 clinical medicine ,Robotic Surgical Procedures ,vaginal cuff dehiscence ,Surgical Wound Dehiscence ,robotic surgery ,medicine ,Humans ,Vaginal bleeding ,Robotic surgery ,postcoital vaginal evisceration ,Evisceration (ophthalmology) ,lcsh:RG1-991 ,Ovarian Neoplasms ,Chemotherapy ,030219 obstetrics & reproductive medicine ,business.industry ,Coitus ,Obstetrics and Gynecology ,medicine.disease ,Endometrial Neoplasms ,Surgery ,Intestinal Diseases ,030220 oncology & carcinogenesis ,Vagina ,Cuff ,Female ,medicine.symptom ,Ovarian cancer ,business - Abstract
Objective We report a rare case of vaginal cuff dehiscence with small bowel evisceration at 7 months post robotic-staging surgery. Case Report A 41-year-old woman was sent to the emergency room with sudden onset of abdominal pain, vaginal bleeding, and vaginal protruding mass after sexual activity. She had a history of synchronous uterine and ovarian cancer treated with robotic-staging surgery 7 months before. Then she received six courses of postoperative adjuvant chemotherapy, and the last chemotherapy ended 1 month ago. At the operation room, some small bowel loops were noted in the vaginal tip with cuff dehiscence and bleeding. After repositioning of the small bowel, a 2.5-cm vaginal cuff dehiscence was repaired transvaginally. The patient recovered well, and is free of disease and has normal sexual activity 2 months after repairs. Conclusion Unusual delayed-type vaginal cuff dehiscence hints the possibility that a combination of robotic surgery and postoperative chemotherapy might result in delayed healing of the vaginal cuff.
- Published
- 2017
24. Gate Dielectric Breakdown in A-InGaZnO Thin Film Transistors With Cu Electrodes.
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Tai, Mao-Chou, Wang, Yu-Xuan, Chang, Ting-Chang, Huang, Hui-Chen, Lin, Chih-Chih, Huang, Bo-Shen, Chang, Han-Yu, Huang, Jen-Wei, and Sze, Simon
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DIELECTRIC breakdown ,STRAY currents ,ELECTRODES ,THIN film transistors ,TRANSISTORS ,HOPPING conduction ,NEGATIVE electrode - Abstract
In this work, the impact of positive bias stress (PBS) on an all copper(Cu)-electrode a-InGaZnO TFT is discussed. Commonly, Cu diffusion from the source/drain electrode after negative bias stress (NBS) leads to degradations in the on-state current and subthreshold swing. However, this study finds that diffusion in the gate insulator layer from the gate electrode suffers from more serious degradation. Eventually, this phenomenon causes a serious breakdown of the gate insulator such that it loses the transistor function. Results indicate that after 2000s of PBS, accumulation of Cu ions forms a filament which dominates the leakage path. During the stress duration, the gate leakage current is monitored and current fittings are adopted to confirm the phenomena. Furthermore, temperature dependencies have verified the results of hopping and Ohmic conduction at different stages of deterioration. Compared to previous literature that focus on the impacts of source/drain electrodes, this work suggests that gate engineering for thin film transistors is more urgent for high-quality display applications. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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25. Platelet Glycoprotein IIb/IIIa Receptor Antagonist (Abciximab) Inhibited Platelet Activation and Promoted Skin Flap Survival after Ischemia/Reperfusion Injury
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Kuo, Yur-Ren, Jeng, Seng-Feng, Wang, Feng-Sheng, Huang, Hui-Chen, Wei, Fu-Chan, and Yang, Kuender D.
- Published
- 2002
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26. A thioredoxin NbTRXh2 from <italic>Nicotiana benthamiana</italic> negatively regulates the movement of <italic>Bamboo mosaic virus</italic>.
- Author
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Chen, I‐Hsuan, Chen, Hui‐Ting, Huang, Ying‐Ping, Shenkwen, Lin‐Ling, Hsu, Yau‐Heiu, Tsai, Ching‐Hsiu, and Huang, Hui‐Chen
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THIOREDOXIN ,NICOTIANA benthamiana ,BAMBOO mosaic virus ,ARABIDOPSIS ,PLANT gene silencing ,PLANT viruses ,PLASMODESMATA - Abstract
Summary: An up‐regulated gene derived from
Bamboo mosaic virus (BaMV)‐infectedNicotiana benthamiana plants was cloned and characterized in this study. BaMV is a single‐stranded, positive‐sense RNA virus. This gene product, designated as NbTRXh2, was matched with sequences of thioredoxin h proteins, a group of small proteins with a conserved active‐site motif WCXPC conferring disulfide reductase activity. To examine how NbTRXh2 is involved in the infection cycle of BaMV, we used the virus‐induced gene silencing technique to knock down NbTRXh2 expression inN. benthamiana and inoculated the plants with BaMV. We observed that, compared with control plants, BaMV coat protein accumulation increased in knockdown plants at 5 days post‐inoculation (dpi). Furthermore, BaMV coat protein accumulation did not differ significantly between NbTRXh2‐knockdown and control protoplasts at 24 hpi. The BaMV infection foci in NbTRXh2‐knockdown plants were larger than those in control plants. In addition, BaMV coat protein accumulation decreased when NbTRXh2 was transiently expressed in plants. These results suggest that NbTRXh2 plays a role in restricting BaMV accumulation. Moreover, confocal microscopy results showed that NbTRXh2‐OFP (NbTRXh2 fused with orange fluorescent protein) localized at the plasma membrane, similar to AtTRXh9, a homologue in Arabidopsis. The expression of the mutant that did not target the substrates failed to reduce BaMV accumulation. Co‐immunoprecipitation experiments revealed that the viral movement protein TGBp2 could be the target of NbTRXh2. Overall, the functional role of NbTRXh2 in reducing the disulfide bonds of targeting factors, encoded either by the host or virus (TGBp2), is crucial in restricting BaMV movement. [ABSTRACT FROM AUTHOR]- Published
- 2018
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27. Survivin counteracts the therapeutic effect of microtubule de-stabilizers by stabilizing tubulin polymers
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Huang Hui Chen, Mohane Selvaraj Coumar, Chun Hei Antonio Cheung, Ching Chuan Kuo, Hsing Pang Hsieh, Chi Yen Chang, and Jang Yang Chang
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Cancer Research ,Indoles ,Survivin ,Down-Regulation ,Antineoplastic Agents ,Apoptosis ,lcsh:RC254-282 ,Microtubules ,Translocation, Genetic ,Inhibitor of Apoptosis Proteins ,Microtubule ,Tubulin ,Cell Line, Tumor ,Humans ,RNA, Small Interfering ,Mitosis ,Caspase ,Caspase 7 ,biology ,Cell growth ,Caspase 3 ,Research ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Cell biology ,Oncology ,Cancer cell ,Cancer research ,biology.protein ,Molecular Medicine ,Colchicine ,Microtubule-Associated Proteins - Abstract
Background Survivin is a dual function protein. It inhibits the apoptosis of cells by inhibiting caspases, and also promotes cell growth by stabilizing microtubules during mitosis. Over-expression of survivin has been demonstrated to induce drug-resistance to various chemo-therapeutic agents such as cisplatin (DNA damaging agent) and paclitaxel (microtubule stabilizer) in cancers. However, survivin-induced resistance to microtubule de-stabilizers such as Vinca alkaloids and Combretastatin A-4 (CA-4)-related compounds were seldom demonstrated in the past. Furthermore, the question remains as to whether survivin plays a dominant role in processing cytokinesis or inhibiting caspases activity in cells treated with anti-mitotic compounds. The purpose of this study is to evaluate the effect of survivin on the resistance and susceptibility of human cancer cells to microtubule de-stabilizer-induced cell death. Results BPR0L075 is a CA-4 analog that induces microtubule de-polymerization and subsequent caspase-dependent apoptosis. To study the relationship between the expression of survivin and the resistance to microtubule de-stabilizers, a KB-derived BPR0L075-resistant cancer cell line, KB-L30, was generated for this study. Here, we found that survivin was over-expressed in the KB-L30 cells. Down-regulation of survivin by siRNA induced hyper-sensitivity to BPR0L075 in KB cells and partially re-stored sensitivity to BPR0L075 in KB-L30 cells. Western blot analysis revealed that down-regulation of survivin induced microtubule de-stabilization in both KB and KB-L30 cells. However, the same treatment did not enhance the down-stream caspase-3/-7 activities in BPR0L075-treated KB cells. Translocation of a caspase-independent apoptosis-related molecule, apoptosis-inducing factor (AIF), from cytoplasm to the nucleus was observed in survivin-targeted KB cells under BPR0L075 treatment. Conclusion In this study, survivin plays an important role in the stability of microtubules, but not with caspases inhibition. Over-expression of survivin counteracts the therapeutic effect of microtubule de-stabilizer BPR0L075 probably by stabilizing tubulin polymers, instead of the inhibition of caspase activity in cancer cells. Besides microtubule-related caspase-dependent cell death, caspase-independent mitotic cell death could be initiated in survivin/BPR0L075 combination treatments. We suggest that combining microtubule de-stabilizers with a survivin inhibitor may attribute to a better clinical outcome than the use of anti-mitotic monotherapy in clinical situations.
- Published
- 2009
28. A Smart and Tangible AR Dress Fitting System.
- Author
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Chiang, Heien-Kun, Chang, Long-Chyr, Kuo, Feng-Lan, and Huang, Hui-Chen
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- 2012
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29. Targeting Hsp90 with small molecule inhibitors induces the over-expression of the anti-apoptotic molecule, survivin, in human A549, HONE-1 and HT-29 cancer cells.
- Author
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Chun Hei Antonio Cheung, Huang-Hui Chen, Li-Ting Cheng, Lyu, Kevin W., Kanwar, Jagat R., and Jang-Yang Chang
- Subjects
- *
CELL death , *PROTEINS , *CANCER cells , *CANCER cell growth , *MITOSIS , *MOLECULAR chaperones , *CELL lines - Abstract
Background: Survivin is a dual functioning protein. It inhibits the apoptosis of cancer cells by inhibiting caspases, and also promotes cancer cell growth by stabilizing microtubules during mitosis. Since the molecular chaperone Hsp90 binds and stabilizes survivin, it is widely believed that down-regulation of survivin is one of the important therapeutic functions of Hsp90 inhibitors such as the phase III clinically trialed compound 17-AAG. However, Hsp90 interferes with a number of molecules that up-regulate the intracellular level of survivin, raising the question that clinical use of Hsp90 inhibitors may indirectly induce survivin expression and subsequently enhance cancer anti-drug responses. The purpose of this study is to determine whether targeting Hsp90 can alter survivin expression differently in different cancer cell lines and to explore possible mechanisms that cause the alteration in survivin expression. Results: Here, we demonstrated that Hsp90 inhibitors, geldanamycin and 17-AAG, induced the over-expression of survivin in three different human cancer cell lines as shown by Western blotting. Increased survivin mRNA transcripts were observed in 17-AAG and geldanamycin-treated HT-29 and HONE-1 cancer cells. Interestingly, real-time PCR and translation inhibition studies revealed that survivin was over-expressed partially through the up-regulation of protein translation instead of gene transcription in A549 cancer cells. In addition, 17-AAG-treated A549, HONE-1 and HT-29 cells showed reduced proteasomal activity while inhibition of 26S proteasome activity further increased the amount of survivin protein in cells. At the functional level, down-regulation of survivin by siRNA further increased the drug sensitivity to 17-AAG in the tested cancer cell lines. Conclusions: We showed for the first time that down-regulation of survivin is not a definite therapeutic function of Hsp90 inhibitors. Instead, targeting Hsp90 with small molecule inhibitors will induce the over-expression of survivin in certain cancer cell lines and subsequently enhances the ability of cell survival in drug-treated situations. The current study suggests that dual inhibition of Hsp90 and survivin may be warranted. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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30. Profile of Urinary Cytokines in Kawasaki Disease: Non-Invasive Markers.
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Huang, Hsin-Chun, Kuo, Ho-Chang, Yu, Hong-Ren, Huang, Hui-Chen, Chang, Jen-Chieh, Lin, I-Chun, and Chen, I-Lun
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MUCOCUTANEOUS lymph node syndrome ,TUMOR necrosis factors ,VASCULAR endothelial growth factors ,URINARY tract infections ,EPIDERMAL growth factor ,BLOOD proteins - Abstract
This cohort study aimed to investigate urinary cytokines expression to help identify a less invasive method of cytokine detection for Kawasaki disease (KD). Patients with confirmed KD were recruited. Patients with fever or urinary tract infection (UTI) were enrolled as control groups. Urinary samples were collected before and 3 days after intravenous immunoglobulin (IVIG) treatment. The levels of cytokines were detected by MILLPLEX
® MAP human multiplex assay. All cytokines, i.e., epidermal growth factor (EGF), interferon (IFN)-γ, interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-13, IL-17A, IL-33, interferon-gamma-induced protein (IP)-10, macrophage inflammatory protein (MIP)-1β, tumor necrosis factor (TNF)-α, and vascular endothelial growth factor (VEGF) except monocyte chemoattractant protein (MCP)-1 were significantly higher in the KD group, compared with the fever-control (FC) group, whereas the expressions of IFN-γ, IL-1β, IL-6, IL-8, IL-17A, IL-33, MCP-1, MIP-1β, and TNF-α were significantly lower in the urine of KD patients, as compared with the UTI group. The expressions of EGF, IFN-γ, IL-8, IL-13, and IL-17A were higher in the urine of KD patients than in the FC group, whereas the level of IL-1β was lower in KD than in the UTI group after age adjustment by logistic regression. Levels of IL-6, IL-8, IL-13, IP-10, and MCP-1 were significantly higher in the pre-IVIG urine of KD patients than in the post-IVIG treatment group. Additionally, urine IL-4 and blood C-reactive protein were higher in the KD group with coronary artery lesion (CAL) than in the non-CAL group. Results of this study provide a new view of urinary cytokine expression in the disease progress of KD, which may help clinicians to predict and prevent morbidity early and non-invasively. [ABSTRACT FROM AUTHOR]- Published
- 2021
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31. Extracorporeal shock waves promote healing of collagenase-induced Achilles tendinitis and increase TGF-β1 and IGF-I expression
- Author
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Chen, Yeung-Jen, Wang, Ching-Jen, Yang, Kuender D., Kuo, Yur-Ren, Huang, Hui-Chen, Huang, Yu-Ting, Sun, Yi-Chih, and Wang, Feng-Sheng
- Subjects
SHOCK waves ,COLLAGENASES ,TENDINITIS ,GENE expression ,CELL proliferation ,ACHILLES tendinitis - Abstract
Extracorporeal shock waves (ESW) have recently been used in resolving tendinitis. However, mechanisms by which ESW promote tendon repair is not fully understood. In this study, we reported that an optimal ESW treatment promoted healing of Achilles tendintis by inducing TGF-β1 and IGF-I. Rats with the collagenease-induced Achilles tendinitis were given a single ESW treatment (0.16 mJ/mm
2 energy flux density) with 0, 200, 500 and 1000 impulses. Achilles tendons were subjected to biomechanical (load to failure and stiffness), biochemical properties (DNA, glycosaminoglycan and hydroxyproline content) and histological assessment. ESW with 200 impulses restored biomechanical and biochemical characteristics of healing tendons 12 weeks after treatment. However, ESW treatments with 500 and 1000 impulses elicited inhibitory effects on tendinitis repair. Histological observation demonstrated that ESW treatment resolved edema, swelling, and inflammatory cell infiltration in injured tendons. Lesion site underwent intensive tenocyte proliferation, neovascularization and progressive tendon tissue regeneration. Tenocytes at the hypertrophied cellular tissue and newly developed tendon tissue expressed strong proliferating cell nuclear antigen (PCNA) after ESW treatment, suggesting that physical ESW could increase the mitogenic responses of tendons. Moreover, the proliferation of tenocytes adjunct to hypertrophied cell aggregate and newly formed tendon tissue coincided with intensive TGF-β1 and IGF-I expression. Increasing TGF-β1 expression was noted in the early stage of tendon repair, and elevated IGF-I expression was persisted throughout the healing period. Together, low-energy shock wave effectively promoted tendon healing. TGF-β1 and IGF-I played important roles in mediating ESW-stimulated cell proliferation and tissue regeneration of tendon. [Copyright &y& Elsevier]- Published
- 2004
- Full Text
- View/download PDF
32. Pertussis toxin-sensitive Gαi protein and ERK-dependent pathways mediate ultrasound promotion of osteogenic transcription in human osteoblasts1<FN ID="FN1"><NO>1</NO>No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subjects of this article.</FN>
- Author
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Chen, Yeung-Jen, Wang, Ching-Jen, Yang, Kuender D., Chang, Per-Rong, Huang, Hui-Chen, Huang, Yu-Ting, Sun, Yi-Chih, and Wang, Feng-Sheng
- Subjects
BONE cells ,MECHANORECEPTORS ,BIOCHEMICAL genetics - Abstract
Bone cells respond to mechanical stimulation via mechanoreceptors and convert biophysical stimulation into biochemical signals that alter gene expression and cellular adaptation. Pulsed acoustic energy treatment raises membrane potential and induces osteogenic activity. How membrane-bound osteoblast mechanoreceptors convert physical ultrasound (US) stimuli into osteogenic responses is not fully understood. We demonstrated that low-intensity pulsed US treatment (200-μs pulse, 1 kHz, 30 mW/cm
2 ) elevated Cbfa1/Runx2 mRNA expression and progressively promoted osteocalcin mRNA expression in human osteoblasts. Pretreatment with pertussis toxin (PTX), but not with cholera toxin, suppressed US-augmented osteogenic transcription. This indicated that Gi proteins, but not Gs proteins, were involved in US promotion of osteogenic transcription. Further studies demonstrated US treatment could rapidly increase PTX-sensitive Gαi protein levels and subsequently enhanced phosphorylation of extracellular signal-regulated kinase (ERK). PTX pretreatment significantly reduced US promotion of ERK activation. Moreover, inhibition of ERK activity by PD98059 suppressed US augmentation of Cbfa1/Runx2 and osteocalcin mRNA expression. Membranous Gαi proteins and cytosolic ERK pathways acted as potent mechanosensitive signals in the response of osteoblasts to pulsed US stimulation. [Copyright &y& Elsevier]- Published
- 2003
- Full Text
- View/download PDF
33. Pertussis toxin-sensitive Gαi protein and ERK-dependent pathways mediate ultrasound promotion of osteogenic transcription in human osteoblasts11No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subjects of this article
- Author
-
Chen, Yeung-Jen, Wang, Ching-Jen, Yang, Kuender D, Chang, Per-Rong, Huang, Hui-Chen, Huang, Yu-Ting, Sun, Yi-Chih, and Wang, Feng-Sheng
- Subjects
Cbfa1/Runx2 ,Gαi protein ,Pertussis toxin ,Osteoblast ,Low-intensity pulsed ultrasound ,Extracellular signal-regulated kinase - Abstract
Bone cells respond to mechanical stimulation via mechanoreceptors and convert biophysical stimulation into biochemical signals that alter gene expression and cellular adaptation. Pulsed acoustic energy treatment raises membrane potential and induces osteogenic activity. How membrane-bound osteoblast mechanoreceptors convert physical ultrasound (US) stimuli into osteogenic responses is not fully understood. We demonstrated that low-intensity pulsed US treatment (200-μs pulse, 1 kHz, 30 mW/cm2) elevated Cbfa1/Runx2 mRNA expression and progressively promoted osteocalcin mRNA expression in human osteoblasts. Pretreatment with pertussis toxin (PTX), but not with cholera toxin, suppressed US-augmented osteogenic transcription. This indicated that Gi proteins, but not Gs proteins, were involved in US promotion of osteogenic transcription. Further studies demonstrated US treatment could rapidly increase PTX-sensitive Gαi protein levels and subsequently enhanced phosphorylation of extracellular signal-regulated kinase (ERK). PTX pretreatment significantly reduced US promotion of ERK activation. Moreover, inhibition of ERK activity by PD98059 suppressed US augmentation of Cbfa1/Runx2 and osteocalcin mRNA expression. Membranous Gαi proteins and cytosolic ERK pathways acted as potent mechanosensitive signals in the response of osteoblasts to pulsed US stimulation.
- Full Text
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34. A novel NRF2/ARE inhibitor gossypol induces cytotoxicity and sensitizes chemotherapy responses in chemo-refractory cancer cells.
- Author
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Ya-Chu Tang, Hsin-Huei Chang, Huang-Hui Chen, Jau-Ying Yao, Yu-Tsen Chen, Yung-Jen Chuang, Jang-Yang Chang, and Ching-Chuan Kuo
- Subjects
- *
REVERSE transcriptase polymerase chain reaction , *WESTERN immunoblotting , *GENE expression , *CELLULAR signal transduction , *CELL survival , *TRANSCRIPTION factors , *PLANT extracts , *CELL lines , *POLYMERASE chain reaction , *MOLECULAR structure , *DRUG resistance in cancer cells , *CHEMICAL inhibitors - Abstract
NRF2/ARE signaling pathway is a principal regulator of cellular redox homoeostasis. The stress-induced transcription factor, NRF2, can shield cells from the oxidative damages via binding to the consensus antioxidant-responsive element (ARE) and driving several cyto-protective genes expression. Increasing evidence indicated that aberrant activation of NRF2 in malignant cells may support their survival through various pathways to detoxify chemotherapy drugs, attenuate drug-induced oxidative stress, or induce drug efflux, all of which are crucial in developing drug resistance. Accordingly, NRF2 is a potential drug target for improving the effectiveness of chemotherapy and to reverse drug resistance in cancer cells. A stable ARE-driven reporter human head and neck squamous cell carcinoma (HNSCC) cell line, HSC3-ARE9, was established and utilized to screen novel NRF2 inhibitors from a compound library. The cotton plant derived phenolic aldehyde-gossypol was selected for further analyses. The effects of gossypol in cancer cells were determined by western blotting, RT-qPCR, clonogenic assay, and cell viability assays. The gossypol-responsive gene expression levels were assessed in the Oncomine database. The effects of gossypol on conferring chemo-sensitization were evaluated in eto- poside-resistant and cisplatin-resistant cancer cells. Our study is the first to identify that gossypol is effective to reduce both basal and NRF2 activator tert-butylhydroquinone (t-BHQ)-induced ARE-luciferase activity. Gossypol diminishes NRF2 protein stability and thereby leads to the suppression of NRF2/ARE pathway, which resulted in decreasing the expression levels of NRF2 downstream genes in both time- and dose-dependent manners. Inhibition of NRF2 by gossypol significantly decreases cell viabilities in human cancer cells. In addition, we find that gossypol re-sensitizes topoisomerase II poison treatment in etoposide-resistant cancer cells via suppression of NRF2/ABCC1 axis. Moreover, gossypol suppresses NRF2-mediated G6PD expression thereby leads to induce synthetic lethality with cisplatin not only in parental cancer cells but also in cisplatin-resistant cancer cells. These findings suggest that gossypol is a novel NRF2/ARE inhibitor, and can be a potential adjuvant chemotherapeutic agent for treatment of chemo-refractory tumor. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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- View/download PDF
35. An alternative method for predicting organic solute volatilization rates under gas and liquid turbulence
- Author
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Chao, Huan-Ping, Lee, Jiunn-Fwu, Lee, Chung-Kung, and Huang, Hui-Chen
- Subjects
- *
ORGANIC compounds , *CARBON compounds , *MASS transfer , *THERMODYNAMICS , *TURBULENCE - Abstract
Abstract: A method for predicting organic compound volatilization rates under turbulent liquid and gas conditions is developed. The reference compounds are classified according to their physico-chemical properties. The mass transfer coefficient (K OL) ratios for organic solutes to the reference compounds are constant for a wide range of environmental conditions, including liquid or air turbulence, or both at once. The obtained results indicate that when the environmental conditions are the same the determination of the volatilization rates is strongly dependent on the solute properties and the chemical structure. The presented method can more effectively estimate the volatilization rates of the solutes than the traditional one under various environmental conditions especially for low volatility solutes. The advantages and disadvantages of the traditional method are also discussed. [Copyright &y& Elsevier]
- Published
- 2005
- Full Text
- View/download PDF
36. Activation of extracellular signal-regulated kinase (ERK) and p38 kinase in shock wave-promoted bone formation of segmental defect in rats
- Author
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Chen, Yeung-Jen, Kuo, Yur-Ren, Yang, Kuender D., Wang, Ching-Jen, Sheen Chen, Shyr-Ming, Huang, Hui-Chen, Yang, Ya-Ju, Yi-Chih, Sun, and Wang, Feng-Sheng
- Subjects
- *
BONE regeneration , *GROWTH factors , *BONE morphogenetic proteins , *EXTRACORPOREAL shock wave therapy , *STEM cells - Abstract
Extracorporeal shock waves (ESW) have recently been used in bone repair. Extracellular signal-regulated kinase (ERK) and p38 kinase are found to act as important mediators for osteogenic factor and mechanical-stimulated proliferation and differentiation of bone-forming cells. A previous study reported that ESW promoted healing of segmental defects in rats by inducing bone morophogenetic proteins (Bone 32 (2003) 387–396) and stimulating osteogenic differentiation of mesenchymal stem cells. In this study, we found that ERK and p38 activation was involved in ESW-augmented bone regeneration of segmental defects. ESW treatment (0.16 mJ/mm2, 1 Hz, 500 impulses) rapidly promoted [3H]-thymidine uptake in 1 day and progressively increased alkaline phosphatase activity, collagen I, II, and osteocalcin synthesis in callus organ culture within 14 days after treatment. Results of [γ-32P]-phosphotransferase activity assay showed that ERK and p38 in calluses were rapidly activated 1 day and 7 days after ESW treatment, respectively. Histological observation showed that segmental defects subjected to ESW treatment underwent typical bone formation (mesenchymal cell aggregation, hypertrophic cartilage, and endochondral/intramembrane ossification). Intensive bone formation coincided with evident expression of phosphorylated ERK and p38. Moreover, expression of phosphorylated ERK persisted in mesenchymal, chondral, and osteoblastic cells at newly developed bone and cartilage, and the expression of activated p38 was evident on chondral cells located at hypertrophic cartilage. Our findings suggest that mitogen-activated protein kinases (MAPK) regulate the stimulation of biophysical ESW, triggering mitogenic and osteogenic responses in the defects. ERK phosphorylation is active throughout the period of ESW-induced bone regeneration. p38 activation most likely plays an important role in signaling cartilage formation in callus. [Copyright &y& Elsevier]
- Published
- 2004
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- View/download PDF
37. A thioredoxin NbTRXh2 from Nicotiana benthamiana negatively regulates the movement of Bamboo mosaic virus.
- Author
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Chen IH, Chen HT, Huang YP, Huang HC, Shenkwen LL, Hsu YH, and Tsai CH
- Subjects
- Gene Silencing physiology, Plant Proteins genetics, Thioredoxins genetics, Nicotiana genetics, Plant Proteins metabolism, Potexvirus pathogenicity, Thioredoxins metabolism, Nicotiana metabolism
- Abstract
An up-regulated gene derived from Bamboo mosaic virus (BaMV)-infected Nicotiana benthamiana plants was cloned and characterized in this study. BaMV is a single-stranded, positive-sense RNA virus. This gene product, designated as NbTRXh2, was matched with sequences of thioredoxin h proteins, a group of small proteins with a conserved active-site motif WCXPC conferring disulfide reductase activity. To examine how NbTRXh2 is involved in the infection cycle of BaMV, we used the virus-induced gene silencing technique to knock down NbTRXh2 expression in N. benthamiana and inoculated the plants with BaMV. We observed that, compared with control plants, BaMV coat protein accumulation increased in knockdown plants at 5 days post-inoculation (dpi). Furthermore, BaMV coat protein accumulation did not differ significantly between NbTRXh2-knockdown and control protoplasts at 24 hpi. The BaMV infection foci in NbTRXh2-knockdown plants were larger than those in control plants. In addition, BaMV coat protein accumulation decreased when NbTRXh2 was transiently expressed in plants. These results suggest that NbTRXh2 plays a role in restricting BaMV accumulation. Moreover, confocal microscopy results showed that NbTRXh2-OFP (NbTRXh2 fused with orange fluorescent protein) localized at the plasma membrane, similar to AtTRXh9, a homologue in Arabidopsis. The expression of the mutant that did not target the substrates failed to reduce BaMV accumulation. Co-immunoprecipitation experiments revealed that the viral movement protein TGBp2 could be the target of NbTRXh2. Overall, the functional role of NbTRXh2 in reducing the disulfide bonds of targeting factors, encoded either by the host or virus (TGBp2), is crucial in restricting BaMV movement., (© 2017 BSPP AND JOHN WILEY & SONS LTD.)
- Published
- 2018
- Full Text
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38. MicroRNA-142-3p and let-7g Negatively Regulates Augmented IL-6 Production in Neonatal Polymorphonuclear Leukocytes.
- Author
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Huang HC, Yu HR, Hsu TY, Chen IL, Huang HC, Chang JC, and Yang KD
- Subjects
- Adult, Cells, Cultured, Female, Gene Expression Profiling, Gene Expression Regulation, Humans, Immunohistochemistry, Interleukin-6 genetics, Male, MicroRNAs genetics, Reverse Transcriptase Polymerase Chain Reaction, Interleukin-6 metabolism, MicroRNAs metabolism, Neutrophils metabolism
- Abstract
Neonatal PMN are qualitatively impaired in functions, yet they frequently reveal augmented inflammatory reactions during sepsis. Here, we hypothesized that PMN from newborns produce more IL-6 than those from adults under LPS stimulation, in which transcriptional or posttranscriptional regulation is involved in the altered expression. We found that neonatal PMN produced significantly higher IL-6 mRNA and protein than adult PMN. The higher IL-6 expression was not related to transcriptional but posttranscriptional regulation as the IL-6 expression was affected by the addition of cycloheximide but not actinomycin. To examine whether miRNA was involved in the IL-6 regulation of neonatal PMN, we surveyed differential displays of miRNAs that could potentially regulate IL-6 expression before and after LPS stimulation. Four miRNAs: hsa-miR-26a, hsa-miR-26b, hsa-miR-142-3p and hsa-let 7g decreased or increased after LPS treatment for 4 h. Further validation by qRT-PCR identified miR-26b, miR-142-3p and let-7g significantly changed in neonatal PMN after LPS stimulation. The functional verification by transfection of miR-142-3p and let-7g precursors into neonatal PMN significantly repressed the IL-6 mRNA and protein expression, suggesting that miR-142-3p and let-7g negatively regulate IL-6 expression in neonatal PMN. Modulation of miRNA expression may be used to regulate IL-6 production in newborns with altered inflammatory reactions., Competing Interests: Competing Interests: The authors have declared that no competing interest exists.
- Published
- 2017
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39. miRNA-125b regulates TNF-α production in CD14+ neonatal monocytes via post-transcriptional regulation.
- Author
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Huang HC, Yu HR, Huang LT, Huang HC, Chen RF, Lin IC, Ou CY, Hsu TY, and Yang KD
- Subjects
- Adult, Biomarkers metabolism, Blotting, Western, Cells, Cultured, Endotoxins pharmacology, Enzyme-Linked Immunosorbent Assay, Female, Fetal Blood, Flow Cytometry, Gene Expression Profiling, Humans, Infant, Newborn, Lipopolysaccharides pharmacology, Monocytes cytology, Monocytes drug effects, NF-kappa B genetics, NF-kappa B metabolism, Oligonucleotide Array Sequence Analysis, RNA, Messenger genetics, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Lipopolysaccharide Receptors metabolism, MicroRNAs genetics, Monocytes metabolism, RNA Processing, Post-Transcriptional, Tumor Necrosis Factor-alpha metabolism
- Abstract
Neonates, although deficient in cell immunity, frequently reveal sepsis with augmented proinflammatory reactions. Here, we found that neonatal monocytes produced significantly higher TNF-α mRNA and protein than adult monocytes. Assessment of the transcriptional factor found no significant difference of NF-κB p65 level between neonatal and adult monocytes. Addition of Act D to access the half-life of TNF-α mRNA revealed no significant difference of the LPS-induced TNF-α mRNA half-life between them, whereas CHX increased neonatal TNF-α mRNA significantly. This suggests that a post-transcriptional mechanism involves the augmentation of TNF-α production by neonatal monocytes. To examine whether miRNA was involved in the post-transcriptional regulation, differential displays of miRNA array between neonatal and adult MNCs were performed, along with the discovery of hsa-miR-103, hsa-miR-125b, hsa-miR-130a, hsa-miR-454-3p, and hsa-miR-542-3p, which were greater than a twofold decrease or increase after LPS treatment for 4 h. The functional validation identified that miR-125b decreased significantly in association with higher TNF-α expression by neonatal monocytes after LPS stimulation. Transfection of the miR-125b precursor into neonatal monocytes significantly repressed the TNF-α mRNA and protein expression, suggesting that miR-125b negatively regulates TNF-α expression in neonatal monocytes. Modulation of miRNA expression may be used to regulate TNF-α production in newborns with altered proinflammatory reactions.
- Published
- 2012
- Full Text
- View/download PDF
40. Suppressed TGF-beta1 expression is correlated with up-regulation of matrix metalloproteinase-13 in keloid regression after flashlamp pulsed-dye laser treatment.
- Author
-
Kuo YR, Wu WS, Jeng SF, Wang FS, Huang HC, Lin CZ, and Yang KD
- Subjects
- Adolescent, Adult, Child, Collagenases radiation effects, Down-Regulation radiation effects, Female, Humans, Keloid pathology, Male, Matrix Metalloproteinase 1 radiation effects, Matrix Metalloproteinase 13, Middle Aged, Transforming Growth Factor beta radiation effects, Transforming Growth Factor beta1, Up-Regulation radiation effects, Collagenases metabolism, Keloid metabolism, Keloid radiotherapy, Laser Therapy, Matrix Metalloproteinase 1 metabolism, Transforming Growth Factor beta metabolism
- Abstract
Background and Objectives: Flashlamp pulsed-dye lasers (PDLs) has shown effectiveness in the treatment of keloids. In this study, we investigated whether PDL treatments decreased transforming growth factor-beta1 (TGF-beta1)-induction and up-regulation of matrix metalloproteinase (MMP) expression in keloid regression., Study Design/materials and Methods: Keloid tissues obtained from 10 patients with intra-lesional or punch biopsies before and 7 days after PDL treatments [fluence per pulse was 10-18 J/cm2 (mean 14.0 J/cm2)]. Immunohistochemical (IHC) staining of TGF-beta1 and MMP-1 and MMP-13 expressions in keloid tissue was performed. Western blot analysis of MMP-1 and MMP-13 expressions in extracellular matrix was evaluated., Results: IHC staining indicated that expression of TGF-beta1 was significantly reduced in keloid tissues after PDL irradiation. MMP-13 but not MMP-1 expression on IHC staining significantly increased in extracellular matrix of keloid tissues after PDL treatment. Western blot analysis also showed MMP-13 but not MMP-1 significant increased in keloid tissues after PDL treatment., Conclusions: Regression of keloids regressed after PDL treatments are associated with down-regulation of TGF-beta1 expression and up-regulation of MMP-13 activity.
- Published
- 2005
- Full Text
- View/download PDF
41. Nitrosoglutathione promotes flap survival via suppression of reperfusion injury-induced superoxide and inducible nitric oxide synthase induction.
- Author
-
Kuo YR, Wang FS, Jeng SF, Lutz BS, Huang HC, and Yang KD
- Subjects
- Animals, Antioxidants administration & dosage, Antioxidants metabolism, Free Radical Scavengers, Immunohistochemistry, Male, Models, Animal, Nitric Oxide Donors metabolism, Nitric Oxide Synthase antagonists & inhibitors, Nitric Oxide Synthase metabolism, Rats, Rats, Inbred Lew, S-Nitrosoglutathione metabolism, Superoxides antagonists & inhibitors, Superoxides metabolism, Tissue Survival, Nitric Oxide Donors administration & dosage, Reperfusion Injury prevention & control, S-Nitrosoglutathione administration & dosage, Surgical Flaps blood supply
- Abstract
Background: Evidence suggests that failure of flap reconstruction is related to ischemia/reperfusion (I/R)-mediated endothelial damage. Using a rat inferior epigastric artery flap as an I/R injury model, we investigated whether administration of nitrosoglutathione (GSNO), an exogenous nitric oxide (NO) donor, can scavenge superoxide and promote flap survival., Methods: Thirty minutes before flap reperfusion, normal saline, N-acetylcysteine (75 and 150 mg/kg), or GSNO (0.2 and 0.6 mg/kg) was randomly injected into 10 rats. Superoxide, nuclear factor-kappa B (NF-kappa B) activation, NO synthase (NOS) isoforms, and 3-nitrotyrosine expression in the pedicle vessels as well as survival areas of the flaps were evaluated., Results: I/R injury induced superoxide production, NF-kappa B activation, and inducible NOS (iNOS) expression in the pedicle vessels. GSNO significantly inhibited superoxide production and suppressed NF-kappa B activation, iNOS induction, and 3-nitrotyrosine expression, but up-regulated endothelial NOS expression in the flap vessels. Optimal doses of both GSNO (0.6 mg/kg) and N-acetylcysteine (150 mg/kg) effectively promoted flap survival area (p < 0.001), although there was no significant difference between both groups., Conclusion: Exogenous NO donation by GSNO can scavenge superoxide and suppress iNOS induction, resulting in better flap survival after prolonged ischemia.
- Published
- 2004
- Full Text
- View/download PDF
42. Extracorporeal shock waves promote healing of collagenase-induced Achilles tendinitis and increase TGF-beta1 and IGF-I expression.
- Author
-
Chen YJ, Wang CJ, Yang KD, Kuo YR, Huang HC, Huang YT, Sun YC, and Wang FS
- Subjects
- Achilles Tendon pathology, Achilles Tendon physiopathology, Animals, Biomechanical Phenomena, Collagenases adverse effects, DNA analysis, Disease Models, Animal, Dose-Response Relationship, Radiation, Glycosaminoglycans metabolism, Hydroxyproline metabolism, Rats, Rats, Sprague-Dawley, Tendinopathy chemically induced, Tendinopathy pathology, Tendon Injuries chemically induced, Transforming Growth Factor beta1, Achilles Tendon metabolism, High-Energy Shock Waves therapeutic use, Insulin-Like Growth Factor I biosynthesis, Tendinopathy therapy, Transforming Growth Factor beta biosynthesis, Wound Healing radiation effects
- Abstract
Extracorporeal shock waves (ESW) have recently been used in resolving tendinitis. However, mechanisms by which ESW promote tendon repair is not fully understood. In this study, we reported that an optimal ESW treatment promoted healing of Achilles tendintis by inducing TGF-beta1 and IGF-I. Rats with the collagenease-induced Achilles tendinitis were given a single ESW treatment (0.16 mJ/mm(2) energy flux density) with 0, 200, 500 and 1000 impulses. Achilles tendons were subjected to biomechanical (load to failure and stiffness), biochemical properties (DNA, glycosaminoglycan and hydroxyproline content) and histological assessment. ESW with 200 impulses restored biomechanical and biochemical characteristics of healing tendons 12 weeks after treatment. However, ESW treatments with 500 and 1000 impulses elicited inhibitory effects on tendinitis repair. Histological observation demonstrated that ESW treatment resolved edema, swelling, and inflammatory cell infiltration in injured tendons. Lesion site underwent intensive tenocyte proliferation, neovascularization and progressive tendon tissue regeneration. Tenocytes at the hypertrophied cellular tissue and newly developed tendon tissue expressed strong proliferating cell nuclear antigen (PCNA) after ESW treatment, suggesting that physical ESW could increase the mitogenic responses of tendons. Moreover, the proliferation of tenocytes adjunct to hypertrophied cell aggregate and newly formed tendon tissue coincided with intensive TGF-beta1 and IGF-I expression. Increasing TGF-beta1 expression was noted in the early stage of tendon repair, and elevated IGF-I expression was persisted throughout the healing period. Together, low-energy shock wave effectively promoted tendon healing. TGF-beta1 and IGF-I played important roles in mediating ESW-stimulated cell proliferation and tissue regeneration of tendon.
- Published
- 2004
- Full Text
- View/download PDF
43. Flashlamp pulsed dye laser (PDL) suppression of keloid proliferation through down-regulation of TGF-beta1 expression and extracellular matrix expression.
- Author
-
Kuo YR, Jeng SF, Wang FS, Chen TH, Huang HC, Chang PR, and Yang KD
- Subjects
- Adolescent, Adult, Child, Collagen Type III metabolism, Down-Regulation, Female, Fibroblasts metabolism, Humans, Keloid metabolism, Male, Middle Aged, Proliferating Cell Nuclear Antigen metabolism, Treatment Outcome, Extracellular Matrix metabolism, Keloid radiotherapy, Laser Therapy, Transforming Growth Factor beta metabolism
- Abstract
Background and Objectives: Keloids have been treated with flashlamp pulsed dye lasers (PDLs) with good results. We investigated whether PDL treatments induced keloid regression by decreasing growth factor-beta(1) (TGF-beta(1)) induction, thereby reducing fibroblast proliferation and collagen deposition., Study Design/materials and Methods: Clinical evaluation and photography documented keloid height/texture, erythema, and pliability before and after PDL treatments scheduled at 2-month intervals in 30 patients. Fluence per pulse was 10-18 J/cm(2) (mean 14.0 J/cm(2)). Immunohistochemical (IHC) staining of TGF-beta(1), proliferating cell nuclear antigen (PCNA), and collagen (types I and III) in extra-cellular matrix was performed on 10 intra-lesional or punch biopsies obtained before and 7 days after PDL treatments., Results: Twelve months after final PDL treatments, keloid regression ( >/= 50%) had occurred in 26/30 patients in whom erythema and surface irregularities had been reduced and pliability had been increased. In 4/30 patients, no changes in keloids had occurred after 12 months. Multiple treatments ( > 6) yielded better results than fewer treatments: 79% versus 50%, respectively. Marked keloid regression ( >/= 90%) occurred in two patients who had received more than 10 treatments. IHC staining indicated that expression of TGF-beta(1), PCNA and collagen type III, but not type I, was significantly reduced in keloid fibroblasts after PDL irradiation., Conclusions: Keloids regressed following PDL-induced reduction in TGF-beta(1) expression, fibroblast proliferation, and collagen type III deposition. More than six PDL treatments at 2-month intervals provided the best results., (Copyright 2004 Wiley-Liss, Inc.)
- Published
- 2004
- Full Text
- View/download PDF
44. Pertussis toxin-sensitive Galphai protein and ERK-dependent pathways mediate ultrasound promotion of osteogenic transcription in human osteoblasts.
- Author
-
Chen YJ, Wang CJ, Yang KD, Chang PR, Huang HC, Huang YT, Sun YC, and Wang FS
- Subjects
- Cell Line, Core Binding Factor Alpha 1 Subunit, Humans, Lipopolysaccharides pharmacology, Osteoblasts radiation effects, Osteocalcin genetics, Osteogenesis genetics, Osteogenesis radiation effects, RNA, Messenger biosynthesis, Signal Transduction, Transcription Factors genetics, Transcription, Genetic drug effects, Virulence Factors, Bordetella pharmacology, GTP-Binding Protein alpha Subunits, Gi-Go metabolism, Mitogen-Activated Protein Kinases metabolism, Neoplasm Proteins, Osteoblasts metabolism, Transcription, Genetic radiation effects, Ultrasonics
- Abstract
Bone cells respond to mechanical stimulation via mechanoreceptors and convert biophysical stimulation into biochemical signals that alter gene expression and cellular adaptation. Pulsed acoustic energy treatment raises membrane potential and induces osteogenic activity. How membrane-bound osteoblast mechanoreceptors convert physical ultrasound (US) stimuli into osteogenic responses is not fully understood. We demonstrated that low-intensity pulsed US treatment (200-micros pulse, 1 kHz, 30 mW/cm2) elevated Cbfa1/Runx2 mRNA expression and progressively promoted osteocalcin mRNA expression in human osteoblasts. Pretreatment with pertussis toxin (PTX), but not with cholera toxin, suppressed US-augmented osteogenic transcription. This indicated that Gi proteins, but not Gs proteins, were involved in US promotion of osteogenic transcription. Further studies demonstrated US treatment could rapidly increase PTX-sensitive Galphai protein levels and subsequently enhanced phosphorylation of extracellular signal-regulated kinase (ERK). PTX pretreatment significantly reduced US promotion of ERK activation. Moreover, inhibition of ERK activity by PD98059 suppressed US augmentation of Cbfa1/Runx2 and osteocalcin mRNA expression. Membranous Galphai proteins and cytosolic ERK pathways acted as potent mechanosensitive signals in the response of osteoblasts to pulsed US stimulation.
- Published
- 2003
- Full Text
- View/download PDF
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