30 results on '"Huang, Qiaorong"'
Search Results
2. Elastically accelerating lookup on virtual SDN flow tables for software-defined cloud gateways
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Xiong, Bing, Wu, Jing, Huang, Qiaorong, Zhao, Jinyuan, Tang, Qiang, Zhang, Jin, Yang, Kun, and Li, Keqin
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- 2024
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3. Gene expression networks involved in multiple cellular programs coexist in individual hepatocellular cancer cells
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Zhao, Jin, Lu, Ran, Jin, Chen, Li, Siying, Chen, Yulin, Huang, Qiaorong, Li, Xue, Meng, Wentong, Wu, Hong, Wen, Tianfu, and Mo, Xianming
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- 2023
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4. Polymeric dual-modal imaging nanoprobe with two-photon aggregation-induced emission for fluorescence imaging and gadolinium-chelation for magnetic resonance imaging
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Xiao, Xueyang, Cai, Hao, Huang, Qiaorong, Wang, Bing, Wang, Xiaoming, Luo, Qiang, Li, Yinggang, Zhang, Hu, Gong, Qiyong, Ma, Xuelei, Gu, Zhongwei, and Luo, Kui
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- 2023
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5. Prognostic value and biological function of LRRN4 in colorectal cancer
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Xu, Cheng, Chen, Yulin, Long, Feiwu, Ye, Junman, Li, Xue, Huang, Qiaorong, Yao, Dejiao, Wang, Xiaoli, Zhao, Jin, Meng, Wentong, Mo, Xianming, Lu, Ran, Fan, Chuanwen, and Zhang, Tao
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- 2022
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6. An integrated map of fibroblastic populations in human colon mucosa and cancer tissues
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Li, Siying, Lu, Ran, Shu, Linjuan, Chen, Yulin, Zhao, Jin, Dai, Junlong, Huang, Qiaorong, Li, Xue, Meng, Wentong, Long, Feiwu, Li, Yuan, Fan, Chuanwen, Zhou, Zongguang, and Mo, Xianming
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- 2022
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7. Integration of Flow Cytometry and Computational Analysis to Dissect the Epidermal Cellular Subsets in Keloids that Correlate with Recurrence
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Zhang, Lidan, Luo, Huaxiu, Meng, Wentong, Cen, Ying, Huang, Qiaorong, Li, Huifang, Mo, Xianming, and Chen, Junjie
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- 2021
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8. Computational flow cytometric analysis to detect epidermal subpopulations in human skin
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Zhang, Lidan, Cen, Ying, Huang, Qiaorong, Li, Huifang, Mo, Xianming, Meng, Wentong, and Chen, Junjie
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- 2021
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9. Chloroquine Alleviates Atherosclerosis by Modulating Regulatory T Cells Through the ATM/AMPK/mTOR Signaling Pathway in ApoE−/− Mice.
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Liu, Dan, Zhang, Yonggang, Zhang, Yiyi, Huang, Qiaorong, Meng, Wentong, Gao, Jinhang, Mo, Xianming, Tian, Haoming, and Li, Sheyu
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REGULATORY T cells ,AMP-activated protein kinases ,CELLULAR signal transduction ,CHLOROQUINE ,ATAXIA telangiectasia - Abstract
Background Clinical observation suggests the atheroprotective effect of chloroquine and its derivatives, while its mechanism remains unclear. This study aimed to observe the protective effect of chloroquine against atherosclerosis and explore the underlying mechanism. Methods Ataxia telangiectasia mutated (ATM) wild-type or haploinsufficient apolipoprotein-E-knockout (ATM
+/+ ApoE−/− or ATM+/− ApoE−/− ) mice were treated with different dosages of chloroquine. Anti-CD25 antibody was used to deplete natural Tregs in ATM+/+ ApoE−/− mice. The atherosclerotic burden in different groups of mice was comprehensively evaluated by H&E staining and Masson staining. The effect of chloroquine on the regulatory T cells (Tregs) was assessed in vivo and in vitro by flow cytometry and immunohistochemical staining. The expression of related proteins was detected by real-time polymerase chain reaction and western blotting. Results In ATM+/+ ApoE−/− mice, chloroquine alleviated atherosclerotic lesions, stabilized the plaque, and increased Treg counts in the atherosclerotic lesions and spleens. However, in ATM haploinsufficient mice (ATM+/− ApoE−/− ), chloroquine no longer prevented atherosclerosis or impacted Treg counts. Abolishing Treg cells using an anti-CD25 antibody in vivo abrogated the atheroprotective effect of chloroquine. In vitro, chloroquine promoted the differentiation of Tregs from naïve T cells, which was accompanied by enhanced ATM/AMP-activated protein kinase (AMPK) activity and reduced downstream mammalian target of rapamycin (mTOR) activity. Discussion These findings suggest that chloroquine ameliorates atherosclerosis and stabilizes plaque by modulating Tregs differentiation through the regulation of the ATM/AMPK/mTOR pathway. [ABSTRACT FROM AUTHOR]- Published
- 2023
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10. Human circulating and tissue gastric cancer stem cells display distinct epithelial–mesenchymal features and behaviors
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Zhang, Shengliang, Shang, Yanna, Chen, Tie, Zhou, Xin, Meng, Wengtong, Fan, Chuanwen, Lu, Ran, Huang, Qiaorong, Li, Xue, Hong, Xu, Zhou, Zongguang, Hu, Jiankun, and Mo, Xianming
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- 2017
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11. Formaldehyde degradation of waterborne paints over S- and C-doped titanium dioxide under visible light.
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Yao, Kang, Meng, Weijun, Huang, Qiaorong, Wang, Wukang, Che, Xihong, Fang, Hong, and Duan, Ran
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- 2023
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12. Spectrum Analysis of Albinism Genes in a Large Cohort of Chinese Index Patients
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Wei, Aihua, Zhang, Tianjiao, Yuan, Yefeng, Qi, Zhan, Bai, Dayong, Zhang, Yingzi, Zhang, Yunlan, Liu, Teng, Huang, Qiaorong, Yang, Xiumin, and Li, Wei
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- 2022
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13. Dual stimuli-responsive dendronized prodrug derived from poly(oligo-(ethylene glycol) methacrylate)-based copolymers for enhanced anti-cancer therapeutic effect.
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Luo, Qiang, Lin, Ling, Huang, Qiaorong, Duan, Zhenyu, Gu, Lei, Zhang, Hu, Gu, Zhongwei, Gong, Qiyong, and Luo, Kui
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COPOLYMERS ,ANTINEOPLASTIC agents ,METHACRYLATES ,TREATMENT effectiveness ,POLYETHYLENE glycol ,POLYESTERS - Abstract
In this study, we developed an enzyme- and pH-responsive dendronized poly(oligo-(ethylene glycol) methacrylate) (pOEGMA)-doxorubicin (DOX) polymeric prodrug, which combined the pOEGMA structure with a degradable peptide dendron. The introduction of the dendron in the prodrug hindered the entanglement of brush oligo-(ethylene glycol) (OEG) chains, allowed the prodrug to possess dual stimuli-responsiveness, and mediated self-assembly of the polymeric prodrug to form stable nanoparticles (NPs). Brush conformation of polyethylene glycol (PEG) side chains endowed the NPs with long-term circulation with a half-life of 16.0 h. The dual-responsive dendritic structure enhanced cellular uptake of NPs and facilitated drug release in response to overexpressed cathepsin B and an acidic pH in the tumor microenvironment, resulting in an enhanced therapeutic effect with a tumor inhibition rate of 72.9% for 4T1 tumor-bearing mice. The NPs were demonstrated to possess great hemocompatibility and biosafety. Therefore, this strategy could provide great insight for the design of poly(oligo-(ethylene glycol) methacrylate)-based copolymers as drug delivery carriers. We propose a dual-stimuli-responsive dendronized strategy for improving the cancer therapeutic effect of the poly(oligo-(ethylene glycol) methacrylate) (pOEGMA)-based drug conjugates. The introduction of the functional dendron promotes self-assembly of the polymeric prodrug into nanoparticles, hindering the entanglement of brush oligo-(ethylene glycol) (OEG) chains in the conjugated drugs. The obtained poly OEGMA-GFLG-Dendron-NH-N=DOX nanoparticles maintains long circulation, while addresses the drug release issue due to the presence of high-density PEG. The drug delivery system exhibits a high therapeutic potentcy with negligible side effects. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2022
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14. Circulating Tissue Factor-Positive Procoagulant Microparticles in Patients with Type 1 Diabetes
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Zhang, Chenghui, Ou, Qing, Gu, Yan, Cheng, Gaiping, Du, Rong, Yuan, Li, Cordiner, Ruth LM, Kang, Deying, Zhang, Jiaying, Huang, Qiaorong, Yu, Chuan, Kang, Li, Wang, Xuan, Sun, Xin, Mo, Xianming, Tian, Haoming, Pearson, Ewan R, Meng, Wentong, and Li, Sheyu
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microparticles ,congenital, hereditary, and neonatal diseases and abnormalities ,diabetic retinopathy ,Endokrinologi och diabetes ,nutritional and metabolic diseases ,Endocrinology and Diabetes ,skin and connective tissue diseases ,tissue factor ,Targets and Therapy [Diabetes, Metabolic Syndrome and Obesity] ,type 1 diabetes mellitus ,Original Research - Abstract
Chenghui Zhang,1,2,* Qing Ou,1,* Yan Gu,1,* Gaiping Cheng,3 Rong Du,1,2 Li Yuan,1 Ruth LM Cordiner,4 Deying Kang,5 Jiaying Zhang,6 Qiaorong Huang,7 Chuan Yu,8 Li Kang,9 Xuan Wang,4,10 Xin Sun,5 Xianming Mo,7 Haoming Tian,1 Ewan R Pearson,4 Wentong Meng,7 Sheyu Li1,4 1Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China; 2Department of Endocrinology and Metabolism, Hospital of Chengdu Office of People’s Government of Tibetan Autonomous Region, Chengdu 610041, People’s Republic of China; 3Department of Clinical Nutrition, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China; 4Division of Population Health and Genomics, Ninewells Hospital and School of Medicine, University of Dundee, Dundee DD1 9SY, Scotland, UK; 5Chinese Evidence-Based Medicine Center, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China; 6Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China; 7Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, People’s Republic of China; 8Department of Health-Related Social and Behavioral Science, West China School of Public Health, Sichuan University, Chengdu 610041, People’s Republic of China; 9Division of Systems Medicine, Ninewells Hospital and School of Medicine, University of Dundee, Dundee DD1 9SY, Scotland, UK; 10Science for Life Laboratory, Department of Medical Cell Biology, Uppsala University, Uppsala 75123, Sweden*These authors contributed equally to this workCorrespondence: Sheyu LiDepartment of Endocrinology and Metabolism, West China Hospital, Sichuan University, 37# Guoxue Road, Chengdu 610041, ChinaTel +86-13194874843Fax +86-28-85422982Email lisheyu@gmail.comWentong MengLaboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 37# Guoxue Road, Chengdu, Sichuan 610041, People’s Republic of ChinaTel +86-18980601256Fax +86-28-85422982Email mwentong@sina.comAim: To investigate the count of circulating tissue factor-positive (TF+) procoagulant microparticles (MPs) in patients with type 1 diabetes mellitus (T1DM).Methods: This case-control study included patients with T1DM and age and sex-matched healthy volunteers. The counts of phosphatidylserine-positive (PS+) MPs and TF+PS+MPs and the subgroups derived from different cell types were measured in the peripheral blood sample of the two groups using multicolor flow cytometric assay. We compared the counts of each MP between groups as well as the ratio of the TF+PS+MPs and PS+MPs (TF+PS+MPs/PS+MPs).Results: We recruited 36 patients with T1DM and 36 matched healthy controls. Compared with healthy volunteers, PS+MPs, TF+PS+MPs and TF+PS+MPs/PS+MPs were elevated in patients with T1DM (PS+MPs: 1078.5 ± 158.08 vs 686.84 ± 122.04/μL, P
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- 2020
15. The first Hermansky–Pudlak syndrome type 9 patient with two novel variants in Chinese population.
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Liu, Teng, Yuan, Yefeng, Bai, Dayong, Yao, Xingfeng, Zhang, Tianjiao, Huang, Qiaorong, Qi, Zhan, Yang, Lin, Yang, Xiumin, Li, Wei, and Wei, Aihua
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Hermansky‐Pudlak syndrome 9 (HPS‐9) is a recessive disorder caused by BLOC1S6 gene. There are only four variants identified from four HPS‐9 patients so far. Here, we reported the first HPS‐9 patient in a Chinese population. He had brownish‐yellow hair, white skin, brown irises with visual acuity, photophobia and nystagmus. Two novel variants, c.148G>T (p.Glu50*) and c.351dupT (p.Ile118Tyrfs*10) in BLOC1S6 gene were identified by whole‐exome sequencing (WES). Absence of platelet dense granules was found by whole‐mount platelet electron microscopy and Western blotting assays showed the destabilized BLOC‐1 subunits. He had recurrent bruising and was found to have abnormal brain waves by electroencephalogram, but did not develop thrombopenia, immunodeficiency or other symptoms reported in other HPS‐9 patients. This is the first case report of BLOC‐1 mutation in a Chinese population and our findings expand the mutational spectrum of HPS genes. [ABSTRACT FROM AUTHOR]
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- 2021
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16. Prognostic value of CD133+ CD54+ CD44+ circulating tumor cells in colorectal cancer with liver metastasis.
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Fang, Chao, Fan, Chuanwen, Wang, Cun, Huang, Qiaorong, Meng, Wentong, Yu, Yongyang, Yang, Lie, Hu, Jiankun, Li, Yuan, Mo, Xianming, and Zhou, Zongguang
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LIVER metastasis ,COLON cancer ,FLOW cytometry ,METASTASIS ,CANCER treatment ,PATIENTS - Abstract
In the previous study, we had showed the expression of CD133
+ CD54+ CD44+ cellular subpopulation of circulating tumor cells ( CTCs) was significantly associated with liver metastasis of colorectal cancer ( CRC). This study aimed to explore whether this subpopulation of CTCs have a prognostic value in CRC patients. Flow cytometry was used to detect the expression of cellular subpopulations of CTCs with CD133, CD54, and CD44 in 152 CRC patients, between December 2013 and October 2014. The impact of clinicopathological factors and the expression of cellular subpopulations of CTCs on overall survival were then analyzed. CRC patients with liver metastases who underwent resection of the primary tumor accompanied by surgical treatment for metastasis had a better survival than other patients ( P < 0.001). The liver metastatic CRC patients with high expression of CD133+ CD54+ ( P < 0.001), CD133− CD54+ ( P = 0.004), and CD133+ CD44+ CD54+ ( P = 0.003) cellular subpopulations of CTCs had a worse survival than those patients with low expression. Multivariable survival analyses identified carcinoembryonic antigen levels (hazard ratio [ HR] = 3.056; 95% confidence interval [ CI] = 1.354-6.897; P = 0.007), treatment strategy ( HR = 0.212; 95% CI = 0.056-0.808; P = 0.023), and CD133+ CD44+ CD54+ cellular subpopulation of CTCs ( HR = 6.459; 95% CI = 1.461-28.558; P = 0.014) as independent prognostic factors for CRC patients with liver metastasis. CD133+ CD44+ CD54+ cellular subpopulation of CTCs has a prognostic value in CRC patients with liver metastasis, especially in the survival of CRC patients with liver metastasis who did not undergo surgical treatment for metastasis. [ABSTRACT FROM AUTHOR]- Published
- 2017
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17. Endothelium originated from colorectal cancer stem cells constitute cancer blood vessels.
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Shangguan, Wenqi, Fan, Chuanwen, Chen, Xiaolong, Lu, Ran, Liu, Yuan, Li, Yu, Shang, Yanna, Yin, Dongqin, Zhang, Shengliang, Huang, Qiaorong, Li, Xue, Meng, Wentong, Xu, Hong, Zhou, Zongguang, Hu, Jiankun, and Mo, Xianming
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Tumor growth depends on the formation of blood vessels that provide the supply of nutrients and oxygen. Previous data have shown that glioblastoma stem cells are able to give rise to vascular cells to constitute the functional vessels in tumor tissues. However, which kinds of vascular cells are generated from glioblastoma stem cells is largely debated. In addition, there is little evidence showing that the stem cells from other kinds of tumors can produce vascular cells to constitute the functional blood vessels in tumor tissues. Here we show that cancer stem cells of human colorectal carcinomas (Co CSC) can give rise to vascular endothelial cells and compose the vasculatures in cancer tissues. The human-cell-specific nuclear antigen Nu MA
+ vascular endothelial cells were detected in the blood vessels in xenografts derived from Co CSC. Nu MA+ endothelial cells incorporated into functional blood vessels. Our data indicate that the cancer stem cells derived from human colorectal carcinomas have the capacity to generate functional blood vessels and provide a new mechanism for tumor vasculogenesis in carcinoma. [ABSTRACT FROM AUTHOR]- Published
- 2017
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18. Suppression of Epidermal Growth Factor Receptor (EGFR) Expression by Small Hairpin RNA Inhibits the Growth of Human Nonsmall Cell Lung Cancers Bearing Wild-type and Mutant EGFR.
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Zhang, Shuang, Tian, Hongwei, Jiang, Qingyuan, Li, Li, Ding, Yu, Dai, Lei, Xiang, Yu, Shen, Guobo, Cheng, Lin, Huang, Qiaorong, Liu, Yalin, Zhang, Xiaomei, Ma, Yongping, Zhang, Na, Liu, Shengyong, Wang, Wei, Dai, Lixia, Li, Yiming, Zhao, Xia, and Wei, Yuquan
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EPIDERMAL growth factor ,GENE expression ,SMALL cell lung cancer ,PLASMIDS ,LUNG tumors ,SMALL interfering RNA ,XENOGRAFTS ,DRUG resistance in cancer cells - Abstract
In the present study, we have used plasmid-based RNA interference (RNAi) strategy to downregulate the expression of epidermal growth factor receptor (EGFR) in EGFR wild-type (H292) and mutant (H1975) lung tumor models. The targeted knockdown of EGFR by small hairpin RNA not only inhibited growth of H292 xenograft but also inhibited H1975 lung cancer cell and xenograft, which bore L858R/T790M EGFR and was resistant to EGFR tyrosine kinase inhibitors. These data demonstrated that small hairpin RNA was an effective therapy against mutant EGFR-expressing cancer cells and thus considered to be a promising strategy in the treatment of lung cancers. [ABSTRACT FROM AUTHOR]
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- 2011
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19. Correction: Colorectal cancer cells secreting DKK4 transform fibroblasts to promote tumour metastasis.
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Li X, Chen Y, Lu R, Hu M, Gu L, Huang Q, Meng W, Zhu H, Fan C, Zhou Z, and Mo X
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- 2024
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20. Genetic insights into across pancreatitis types: the causal influence of immunoglobulin G N-glycosylation variants on disease risk.
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Chen Y, Li X, Lu R, Lv Y, Ye J, Huang Q, Meng W, Long F, Burman J, Mo X, and Fan C
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- Humans, Acute Disease, Ethanol, Genome-Wide Association Study, Glycosylation, Mendelian Randomization Analysis, Immunoglobulin G, Pancreatitis, Chronic genetics
- Abstract
Background: While a few case-control studies indicated a possible correlation of IgG N-glycosylation patterns with pancreatitis, their restricted sample sizes and methodologies prevented conclusive insights into causality or distinguishing traits across pancreatitis types., Method: We conducted a two-sample Mendelian Randomization (MR) analysis to investigate the causal relationship between 77 IgG N-glycosylation traits and various types of pancreatitis, including acute pancreatitis (AP), chronic pancreatitis (CP), alcohol acute pancreatitis (AAP), and alcohol chronic pancreatitis (ACP). This analysis utilized summary-level data from genome-wide association studies (GWAS), employing methods such as IVW, MR-Egger, and weighted median. To ensure the robustness of our findings, several sensitivity analyses, including Cochran's Q statistic, leave-one-out, MR-Egger intercept, and MR-PRESSO global test were conducted., Result: Our study uncovered the causal relationship between specific IgG N-glycosylation traits and various types of pancreatitis. Notably, an increase in genetically predicted IGP7 levels was associated with a decreased risk of developing AP. For CP, our data suggested a protective effect associated with higher levels of both IGP7 and IGP31, contrasting with increased levels of IGP27 and IGP65, which were linked to a heightened risk. Moreover, in the case of AAP, elevated IGP31 levels were causatively associated with a lower incidence, while higher IGP26 levels correlated with an increased risk for ACP., Conclusion: This study establishes causal relationship between specific IgG N-glycosylation patterns and varying risks of different pancreatitis forms, underscoring their potential as predictive biomarkers. These findings necessitate further exploration into the underlying mechanisms, promising to inform more personalized diagnostic and therapeutic strategies in pancreatitis management., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Chen, Li, Lu, Lv, Ye, Huang, Meng, Long, Burman, Mo and Fan.)
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- 2024
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21. Chloroquine Alleviates Atherosclerosis by Modulating Regulatory T Cells Through the ATM/AMPK/mTOR Signaling Pathway in ApoE -/- Mice.
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Liu D, Zhang Y, Zhang Y, Huang Q, Meng W, Gao J, Mo X, Tian H, and Li S
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- Mice, Animals, T-Lymphocytes, Regulatory metabolism, Chloroquine pharmacology, Chloroquine metabolism, Chloroquine therapeutic use, AMP-Activated Protein Kinases metabolism, Mice, Knockout, ApoE, Signal Transduction, TOR Serine-Threonine Kinases metabolism, Apolipoproteins E metabolism, Apolipoproteins E pharmacology, Apolipoproteins E therapeutic use, Mice, Inbred C57BL, Mammals metabolism, Ataxia Telangiectasia drug therapy, Ataxia Telangiectasia metabolism, Ataxia Telangiectasia pathology, Atherosclerosis drug therapy, Atherosclerosis prevention & control, Plaque, Atherosclerotic drug therapy, Plaque, Atherosclerotic metabolism, Plaque, Atherosclerotic pathology
- Abstract
Background: Clinical observation suggests the atheroprotective effect of chloroquine and its derivatives, while its mechanism remains unclear. This study aimed to observe the protective effect of chloroquine against atherosclerosis and explore the underlying mechanism., Methods: Ataxia telangiectasia mutated (ATM) wild-type or haploinsufficient apolipoprotein-E-knockout (ATM
+/+ ApoE-/- or ATM+/- ApoE-/- ) mice were treated with different dosages of chloroquine. Anti-CD25 antibody was used to deplete natural Tregs in ATM+/+ ApoE-/- mice. The atherosclerotic burden in different groups of mice was comprehensively evaluated by H&E staining and Masson staining. The effect of chloroquine on the regulatory T cells (Tregs) was assessed in vivo and in vitro by flow cytometry and immunohistochemical staining. The expression of related proteins was detected by real-time polymerase chain reaction and western blotting., Results: In ATM+/+ ApoE-/- mice, chloroquine alleviated atherosclerotic lesions, stabilized the plaque, and increased Treg counts in the atherosclerotic lesions and spleens. However, in ATM haploinsufficient mice (ATM+/- ApoE-/- ), chloroquine no longer prevented atherosclerosis or impacted Treg counts. Abolishing Treg cells using an anti-CD25 antibody in vivo abrogated the atheroprotective effect of chloroquine. In vitro, chloroquine promoted the differentiation of Tregs from naïve T cells, which was accompanied by enhanced ATM/AMP-activated protein kinase (AMPK) activity and reduced downstream mammalian target of rapamycin (mTOR) activity., Discussion: These findings suggest that chloroquine ameliorates atherosclerosis and stabilizes plaque by modulating Tregs differentiation through the regulation of the ATM/AMPK/mTOR pathway., Competing Interests: The authors declare no conflicts of interest in this work., (Thieme. All rights reserved.)- Published
- 2023
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22. Worldwide burden attributable to diet high in red meat from 1990 to 2019.
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Liu D, Shi Q, Cheng G, Huang Q, and Li S
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Introduction: Red meat overconsumption is an unhealthy behavior, while its attributed burden and epidemiological pattern remain unclear. This study aimed to describe the status and trend of how the diet high in red meat burdens the world., Material and Methods: We accessed the data of summary exposure values (SEVs), deaths, and disability-adjusted life years (DALYs) with their age-standardized rates in each country from the Global Burden of Disease (GBD) Collaborative Network from 1990 to 2019. We calculated estimated annual percentage changes (EAPCs) to evaluate the trends of the disease burden., Results: The age-standardized SEV rates increased in most of the 21 GBD regions, mainly in the low-middle and middle socio-demographic index (SDI) quantiles from 1990 to 2019, while East Asia increased the most rapidly. In 2019, a diet high in red meat was responsible for 0.9 million (95% uncertainty interval (UI) 0.5 to 1.3 million) deaths and 23.9 million (95% UI 15.6 to 32.0 million) DALYs worldwide. From 1990 to 2019, the total deaths and DALYs attributable to a diet high in red meat increased by over 50%. However, the age-standardized death and DALY rates decreased by 30.3% and 23.5%, respectively, during the study period. The age-standardized death and DALY rates in the middle SDI regions surpassed those in the high SDI regions from 2002. Ischemic heart disease, diabetes mellitus, and colorectal cancer were the main causes of diet high in red meat-related deaths and DALYs., Conclusions: Increasing consumption of red meat remains a global challenge, especially in the low-middle and middle SDI countries., Competing Interests: The authors declare no conflict of interest., (Copyright: © 2022 Termedia & Banach.)
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- 2022
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23. Polymeric dual-modal imaging nanoprobe with two-photon aggregation-induced emission for fluorescence imaging and gadolinium-chelation for magnetic resonance imaging.
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Xiao X, Cai H, Huang Q, Wang B, Wang X, Luo Q, Li Y, Zhang H, Gong Q, Ma X, Gu Z, and Luo K
- Abstract
Nanoprobes that offer both fluorescence imaging (FI) and magnetic resonance imaging (MRI) can provide supplementary information and hold synergistic advantages. However, synthesis of such dual-modality imaging probes that simultaneously exhibit tunability of functional groups, high stability, great biocompatibility and desired dual-modality imaging results remains challenging. In this study, we used an amphiphilic block polymer from (ethylene glycol) methyl ether methacrylate (OEGMA) and N -(2-hydroxypropyl) methacrylamide (HPMA) derivatives as a carrier to conjugate a MR contrast agent, Gd-DOTA, and a two-photon fluorophore with an aggregation-induced emission (AIE) effect, TPBP, to construct a MR/two-photon fluorescence dual-modality contrast agent, Gd-DOTA-TPBP. Incorporation of gadolinium in the hydrophilic chain segment of the OEGMA-based carrier resulted in a high r
1 value for Gd-DOTA-TPBP, revealing a great MR imaging resolution. The contrast agent specifically accumulated in the tumor region, allowing a long enhancement duration for vascular and tumor contrast-enhanced MR imaging. Meanwhile, coupling TPBP with AIE properties to the hydrophobic chain segment of the carrier not only improved its water solubility and reduced its cytotoxicity, but also significantly enhanced its imaging performance in an aqueous phase. Gd-DOTA-TPBP was also demonstrated to act as an excellent fluorescence probe for two-photon-excited bioimaging with higher resolution and greater sensitivity than MRI. Since high-resolution, complementary MRI/FI dual-modal images were acquired at both cellular and tissue levels in tumor-bearing mice after application of Gd-DOTA-TPBP, it has great potential in the early phase of disease diagnosis., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 The Authors.)- Published
- 2022
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24. Identification of a Novel MLPH Missense Mutation in a Chinese Griscelli Syndrome 3 Patient.
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Huang Q, Yuan Y, Gong J, Zhang T, Qi Z, Yang X, Li W, and Wei A
- Abstract
Melanophilin (MLPH) functions as a linker between RAB27A and myosin Va (MYO5A) in regulating skin pigmentation during the melanosome transport process. The MYO5A-MLPH-RAB27A ternary protein complex is required for anchoring mature melanosomes in the peripheral actin filaments of melanocytes for subsequent transfer to adjacent keratinocytes. Griscelli syndrome type 3 (GS3) is caused by mutations in the MLPH gene. So far, only five variants of MLPH associated with GS3 have been reported. Here, we reported the first patient with GS3 in a Chinese population. The proband carried a novel homozygous missense mutation (c.73G>C; p.D25H), residing in the conserved Slp homology domain of MLPH, and presented with hypopigmentation of the hair, eyebrows, and eyelashes. Light microscopy revealed the presence of abnormal pigment clumping in his hair shaft. In silico tools predicted this MLPH variant to be likely pathogenic. Using immunoblotting and immunofluorescence analysis, we demonstrated that the MLPH (D25H) variant had an inhibitory effect on melanosome transport by exhibiting perinuclear melanosome aggregation in melanocytes, and greatly reduced its binding to RAB27A, although the protein level of MLPH in the patient was not changed. Our findings suggest that MLPH (D25H) is a pathogenic variant that expands the genetic spectrum of the MLPH gene., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Huang, Yuan, Gong, Zhang, Qi, Yang, Li and Wei.)
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- 2022
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25. Imbalanced Innate Lymphoid Cells are Associated With Disease Activity and Arthritis Involvement in Patients With Systemic Lupus Erythematosus.
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Jiang Y, Zhao Y, Liu Y, Huang Q, Meng W, Xu H, and Mo X
- Abstract
Objectives: This study aims to evaluate the frequency and absolute number of circulating innate lymphoid cell (ILC) subsets and their associations with clinical and serological features in systemic lupus erythematosus (SLE)., Patients and Methods: We recruited 28 SLE patients (6 males, 22 females; mean age 37.57 years; range, 18 to 56 years) and 13 healthy controls (4 males, 9 females; mean age 32.08 years; range, 19 to 48 years). Circulating ILC subsets were identified by flow cytometry. Associations between all detected cells and SLE disease activity, clinical manifestations, and serum autoantibodies were analyzed., Results: In this study, significantly higher frequencies of ILC2s and ILC3s, lower frequencies of ILC1s, and higher ILC1/ILC3 and ILC1/ILC2 ratios were observed in SLE patients than in healthy controls. The frequencies and number of ILC3s were positively associated with SLE disease activity index 2000 score and anti-double stranded deoxyribonucleic acid titers in patients with SLE. Decreased ILC1 frequencies, increased ILC3 frequencies, and decreased ILC1/ILC3 and ILC2/ILC3 ratios were observed in patients with arthritis compared to those without arthritis., Conclusion: Our results indicated biased altered distributions of circulating ILC subsets in SLE. ILC3s were associated with SLE disease activity, and ILC1s, ILC3s, and ILC1/ILC3 and ILC2/ILC3 ratios were associated with SLE accompanied with arthritis. Taken together, these results suggest that ILCs may serve as cellular biomarkers for disease activity and arthritis involvement in SLE., Competing Interests: Conflict of Interest: The authors declared no conflicts of interest with respect to the authorship and/or publication of this article., (Copyright © 2020, Turkish League Against Rheumatism.)
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- 2020
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26. Circulating Tissue Factor-Positive Procoagulant Microparticles in Patients with Type 1 Diabetes.
- Author
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Zhang C, Ou Q, Gu Y, Cheng G, Du R, Yuan L, Cordiner RL, Kang D, Zhang J, Huang Q, Yu C, Kang L, Wang X, Sun X, Mo X, Tian H, Pearson ER, Meng W, and Li S
- Abstract
Aim: To investigate the count of circulating tissue factor-positive (TF
+ ) procoagulant microparticles (MPs) in patients with type 1 diabetes mellitus (T1DM)., Methods: This case-control study included patients with T1DM and age and sex-matched healthy volunteers. The counts of phosphatidylserine-positive (PS+ ) MPs and TF+ PS+ MPs and the subgroups derived from different cell types were measured in the peripheral blood sample of the two groups using multicolor flow cytometric assay. We compared the counts of each MP between groups as well as the ratio of the TF+ PS+ MPs and PS+ MPs (TF+ PS+ MPs/PS+ MPs)., Results: We recruited 36 patients with T1DM and 36 matched healthy controls. Compared with healthy volunteers, PS+ MPs, TF+ PS+ MPs and TF+ PS+ MPs/PS+ MPs were elevated in patients with T1DM (PS+ MPs: 1078.5 ± 158.08 vs 686.84 ± 122.04/μL, P <0.001; TF+ PS+ MPs: 202.10 ± 47.47 vs 108.33 ± 29.42/μL, P <0.001; and TF+ PS+ MPs/PS+ MPs: 0.16 ± 0.04 vs 0.19 ± 0.05, P = 0.004), mostly derived from platelet, lymphocytes and endothelial cells. In the subgroup analysis, the counts of total and platelet TF+ PS+ MPs were increased in patients with diabetic retinopathy (DR) and with higher HbA1c, respectively., Conclusion: Circulating TF+ PS+ MPs and those derived from platelet, lymphocytes and endothelial cells were elevated in patients with T1DM., Competing Interests: Professor Ruth LM Cordiner reports Rising Star Educational Award from International Diabetes Center, Co-Sponsored by Sanofi. The authors report no other conflicts of interest in this work., (© 2019 Zhang et al.)- Published
- 2019
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27. Prognostic value of CD133 + CD54 + CD44 + circulating tumor cells in colorectal cancer with liver metastasis.
- Author
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Fang C, Fan C, Wang C, Huang Q, Meng W, Yu Y, Yang L, Hu J, Li Y, Mo X, and Zhou Z
- Subjects
- Adult, Aged, Catheter Ablation, Chemoembolization, Therapeutic, Chi-Square Distribution, Colectomy, Colorectal Neoplasms blood, Colorectal Neoplasms mortality, Colorectal Neoplasms surgery, Disease Progression, Disease-Free Survival, Female, Flow Cytometry, Hepatectomy, Humans, Immunophenotyping methods, Kaplan-Meier Estimate, Liver Neoplasms blood, Liver Neoplasms mortality, Liver Neoplasms therapy, Male, Middle Aged, Multivariate Analysis, Neoplasm Recurrence, Local, Neoplastic Cells, Circulating immunology, Proportional Hazards Models, Prospective Studies, Risk Factors, Time Factors, Treatment Outcome, AC133 Antigen blood, Biomarkers, Tumor blood, Colorectal Neoplasms pathology, Hyaluronan Receptors blood, Intercellular Adhesion Molecule-1 blood, Liver Neoplasms secondary, Neoplastic Cells, Circulating pathology
- Abstract
In the previous study, we had showed the expression of CD133
+ CD54+ CD44+ cellular subpopulation of circulating tumor cells (CTCs) was significantly associated with liver metastasis of colorectal cancer (CRC). This study aimed to explore whether this subpopulation of CTCs have a prognostic value in CRC patients. Flow cytometry was used to detect the expression of cellular subpopulations of CTCs with CD133, CD54, and CD44 in 152 CRC patients, between December 2013 and October 2014. The impact of clinicopathological factors and the expression of cellular subpopulations of CTCs on overall survival were then analyzed. CRC patients with liver metastases who underwent resection of the primary tumor accompanied by surgical treatment for metastasis had a better survival than other patients (P < 0.001). The liver metastatic CRC patients with high expression of CD133+ CD54+ (P < 0.001), CD133- CD54+ (P = 0.004), and CD133+ CD44+ CD54+ (P = 0.003) cellular subpopulations of CTCs had a worse survival than those patients with low expression. Multivariable survival analyses identified carcinoembryonic antigen levels (hazard ratio [HR] = 3.056; 95% confidence interval [CI] = 1.354-6.897; P = 0.007), treatment strategy (HR = 0.212; 95% CI = 0.056-0.808; P = 0.023), and CD133+ CD44+ CD54+ cellular subpopulation of CTCs (HR = 6.459; 95% CI = 1.461-28.558; P = 0.014) as independent prognostic factors for CRC patients with liver metastasis. CD133+ CD44+ CD54+ cellular subpopulation of CTCs has a prognostic value in CRC patients with liver metastasis, especially in the survival of CRC patients with liver metastasis who did not undergo surgical treatment for metastasis., (© 2017 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.)- Published
- 2017
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28. Neurons generated from carcinoma stem cells support cancer progression.
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Lu R, Fan C, Shangguan W, Liu Y, Li Y, Shang Y, Yin D, Zhang S, Huang Q, Li X, Meng W, Xu H, Zhou Z, Hu J, Li W, Liu L, and Mo X
- Abstract
Recent evidences show that nervous system acts as a crucial part of cancer microenvironment. Infiltration of nerve fibers into cancer microenvironment has an important active role in cancer progression. The stimulations of both cancer growth and metastasis by members of nervous system such as neurons and glial cells have been demonstrated. However, how the nervous system is built in cancer is largely unknown. Here we show that a fraction of cancer stem cells (CSCs) derived from patients with gastric carcinoma and colorectal carcinoma are capable of producing neurons that are involved in tumor neurogenesis and tumor growth. Cancer stem cell monoclone derived from a single cancer stem cell was able to generate neurons including sympathetic and parasympathetic neurons to take part in the nervous system in cancer tissues. Knocking down the neural cell generating capability of the human CSCs inhibited the growth of xenograft tumors in mouse model. Our data demonstrate that human CSCs are able to produce one of most important components in the cancer microenvironment that are required for cancer development and progression., Competing Interests: The authors declare no conflict of interest.
- Published
- 2017
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29. CD133+CD54+CD44+ circulating tumor cells as a biomarker of treatment selection and liver metastasis in patients with colorectal cancer.
- Author
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Fang C, Fan C, Wang C, Huang Q, Meng W, Yu Y, Yang L, Peng Z, Hu J, Li Y, Mo X, and Zhou Z
- Subjects
- Aged, Area Under Curve, Biomarkers, Colorectal Neoplasms diagnostic imaging, Colorectal Neoplasms therapy, Female, Flow Cytometry, Humans, Liver Neoplasms diagnosis, Liver Neoplasms therapy, Male, Middle Aged, Multimodal Imaging methods, Neoplasm Metastasis, Neoplasm Staging, ROC Curve, AC133 Antigen metabolism, Colorectal Neoplasms metabolism, Colorectal Neoplasms pathology, Hyaluronan Receptors metabolism, Intercellular Adhesion Molecule-1 metabolism, Liver Neoplasms secondary, Neoplastic Cells, Circulating metabolism
- Abstract
Introduction: Liver is the most common site of distant metastasis in colorectal cancer (CRC). Early diagnosis and appropriate treatment selection decides overall prognosis of patients. However, current diagnostic measures were basically imaging but not functional. Circulating tumor cells (CTCs) known as hold the key to understand the biology of metastatic mechanism provide a novel and auxiliary diagnostic strategy for CRC with liver metastasis (CRC-LM)., Results: The expression of CD133+ and CD133+CD54+CD44+ cellular subpopulations were higher in the peripheral blood of CRC-LM patients when compared with those without metastasis (P<0.001). Multivariate analysis proved the association between the expression of CD133+CD44+CD54+ cellular subpopulation and the existence of CRC-LM (P<0.001). The combination of abdominal CT/MRI, CEA and the CD133+CD44+CD54+ cellular subpopulation showed increased detection and discrimination rate for liver metastasis, with a sensitivity of 88.2% and a specificity of 92.4%. Meanwhile, it also show accurate predictive value for liver metastasis (OR=2.898, 95% C.I.1.374-6.110)., Materials and Methods: Flow cytometry and multivariate analysis was performed to detect the expression of cancer initiating cells the correlation between cellular subpopulations and liver metastasis in patients with CRC. The receiver operating characteristic curves combined with the area under the curve were generated to compare the predictive ability of the cellular subpopulation for liver metastasis with current CT and MRI images., Conclusions: The identification, expression and application of CTC subpopulations will provide an ideal cellular predictive marker for CRC liver metastasis and a potential marker for further investigation.
- Published
- 2016
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30. TLR7-expressing cells comprise an interfollicular epidermal stem cell population in murine epidermis.
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Yin C, Zhang T, Qiao L, Du J, Li S, Zhao H, Wang F, Huang Q, Meng W, Zhu H, Bu H, Li H, Xu H, and Mo X
- Subjects
- Aminoquinolines pharmacology, Animals, Cell Culture Techniques, Cell Proliferation drug effects, Cells, Cultured, Epidermis metabolism, Epidermis pathology, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Hair Follicle metabolism, Imiquimod, Keratinocytes cytology, Keratinocytes drug effects, Keratinocytes transplantation, Mice, Mice, Inbred C57BL, Mice, Transgenic, Regeneration, Skin metabolism, Skin pathology, Stem Cell Transplantation, Stem Cells metabolism, Tissue Engineering, Toll-Like Receptor 7 agonists, Toll-Like Receptor 7 genetics, Epidermal Cells, Stem Cells cytology, Toll-Like Receptor 7 metabolism
- Abstract
Normal interfollicular epidermis (IFE) homeostasis is maintained throughout the entire life by its own stem cells that self-renew and generate progeny that undergo terminal differentiation. However, the fine markers of the stem cells in interfollicular epidermis are not well defined yet. Here we found that TLR7 identified the existence of progenitors and interfollicular epidermal stem cells in murine skin. In vitro, TLR7-expressing cells comprised of two subpopulations that were competent to proliferate and exhibited distinct differentiation potentials. Three-dimensional (3D) organotypic culture and skin reconstitution assays showed that TLR7-expressing cells were able to reconstruct the interfollicular epidermis. Finally, TLR7-expressing cells maintained the intact interfollicular epidermal structures revealed in serial transplantation assays in vivo in mice. Taken together, our results suggest that TLR7-expressing cells comprise an interfollicular epidermal stem cell population.
- Published
- 2014
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