Search

BackgroundMany previous studies explored the relationship between diabetes and insulin resistance (IR); however, addressing the research gap where no bibliometric analysis had been conducted to summarize and analyze these publications, we will undertake a comprehensive bibliometric analysis to investigate the current status and emerging trends in publications examining the association between diabetes and IR.MethodsWe retrieved publications related to the interaction between diabetes and IR from the Web of Science Core Collection (WoSCC). By utilizing software such as CiteSpace, VOSviewer, and Excel 2019, we analyzed and extracted relevant information from the literature to identify and delineate the research hotspots and directions in the study of diabetes and IR.ResultsFrom 1900 to 2024, a total of 2,698 publications were included in the bibliometric analysis, showing a steady annual increase in the number of publications. The USA led in this research field, with the Harvard University being a key research institution. The author Olefsky JM, published the most papers;Defronzo RA was the most cited author. DIABETES was the journal with the highest number of published papers and was also the most cited journal. The main discipline in the field of diabetes and IR research was Endocrinology and Metabolism. The most cited article was “Mechanisms linking obesity to insulin resistance and type 2 diabetes (2006)”;“The IDF Diabetes Atlas: Global estimates of diabetes prevalence for 2017 and projections for 2045(2018)” was the most cited reference. “insulin resistance” was the most frequently occurring keyword. The main research hotspots and frontier areas in diabetes and IR research were as follows: (1) The association between IR, diabetes, and obesity was a popular research topic; (2) Cardiovascular diseases secondary to diabetes and IR were another hot topic among researchers; (3) As a core pathological change in diabetes, IR was a major therapeutic target for improving diabetes.ConclusionThis study summarized the research trends and hotspots in the field of diabetes and IR, provided valuable information and insights for scholars who focused on diabetes and IR scientific research, and offered a reference for future research directions.

Background MicroRNA (miR)-99a-5p acts as a tumor suppressor in several tumors, including bladder cancer and breast cancer, but its biological function in oral squamous cell carcinoma (OSCC) is poorly understood. Methods miR-99a-5p expression was determined in OSCC tissues and cell lines using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Cell proliferation was assessed by the Cell Counting Kit-8 assay and colony formation assay. Wound healing and Transwell assays were used to analyze migration and invasion abilities, respectively, in OSCC cells. The luciferase reporter assay, RT-qPCR, and western blotting were used to determine the relationship between miR-99a-5p and isoprenylcysteine carboxylmethyltransferase (ICMT). Results miR-99a-5p expression in OSCC tissues and cell lines was significantly decreased compared with corresponding controls, and was significantly associated with clinical stage and lymph node metastasis in OSCC. Functional assays revealed that miR-99a-5p overexpression significantly inhibited the proliferation, migration, and invasion abilities of CAL-27 and TCA-8113 OSCC cells. miR-99a-5p was found to directly target ICMT, while ICMT restoration reversed the role of miR-99a-5p in OSCC cells. Conclusions Our results indicate that miR-99a-5p-mediates the down-regulation of ICMT, which could be used as a novel potential therapeutic target for OSCC treatment.

Retroviral assembly is a highly coordinated step in the replication cycle. The process is initiated when the newly synthesized Gag and Gag-Pol polyproteins are directed to the inner leaflet of the plasma membrane (PM), where they facilitate the budding and release of immature viral particles. Extensive research over the years has provided crucial insights into the molecular determinants of this assembly step. It is established that Gag targeting and binding to the PM is mediated by interactions of the matrix (MA) domain and acidic phospholipids such as phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). This binding event, along with binding to viral RNA, initiates oligomerization of Gag on the PM, a process mediated by the capsid (CA) domain. Much of the previous studies have focused on human immunodeficiency virus type 1 (HIV-1). Although the general steps of retroviral replication are consistent across different retroviruses, comparative studies revealed notable differences in the structure and function of viral components. In this review, we present recent findings on the assembly mechanisms of Human T-cell leukemia virus type 1 and highlight key differences from HIV-1, focusing particularly on the molecular determinants of Gag–PM interactions and CA assembly.

With the rapid advancement of computer vision, machine learning, and consumer electronics, eye tracking has emerged as a topic of increasing interest in recent years. It plays a key role across diverse domains including human–computer interaction, virtual reality, and clinical and healthcare applications. Near-eye tracking (NET) has recently been developed to possess encouraging features such as wearability, affordability, and interactivity. These features have drawn considerable attention in the health domain, as NET provides accessible solutions for long-term and continuous health monitoring and a comfortable and interactive user interface. Herein, this work offers an inaugural concise review of NET for health, encompassing approximately 70 related articles published over the past two decades and supplemented by an in-depth examination of 30 literatures from the preceding five years. This paper provides a concise analysis of health-related NET technologies from aspects of technical specifications, data processing workflows, and the practical advantages and limitations. In addition, the specific applications of NET are introduced and compared, revealing that NET is fairly influencing our lives and providing significant convenience in daily routines. Lastly, we summarize the current outcomes of NET and highlight the limitations.

ABSTRACTBalancing the potentially serious outcomes of asymptomatic brucellosis and “waiting” for treatment in clinical practice is an urgent issue. Therefore, we assessed the follow-up outcomes and epidemiological characteristics of asymptomatic brucellosis in the absence of treatment to provide evidence-based clinical clues. We searched eight databases in which 3610 studies from 1990 to 2021 were related to the follow-up outcomes of asymptomatic brucellosis. Thirteen studies, involving 107 cases, were finally included. Regarding the follow-up outcomes, we examined the presence or absence of symptoms and decreased serum agglutination test (SAT) titre. During the 0.5–18 months follow-up period, the pooled prevalence of appearing symptomatic was 15.4% (95% CI 2.1%–34.3%), cases that remained asymptomatic were 40.3% (95% CI 16.6%–65.8%), and decreased SAT titre was observed in 36.5% (95% CI 11.6%–66.1%). Subgroup analysis indicated that the pooled prevalence of appearing symptomatic with follow-up times of less than 6 months, 6–12 months, and 12–18 months was 11.5%, 26.4%, and 47.6%, respectively. The student subgroup had a higher prevalence of symptoms (46.6%) than the occupational and family populations. In conclusion, asymptomatic brucellosis has a high likelihood of appearing symptomatic and its severity may be underestimated. Active screening of occupational and family populations should be enhanced, and special attention should be paid to high-titre students for early intervention, if necessary. Additionally, future prospective, long-term, and large-sample follow-up studies are essential.

IntroductionGanmai Dazao Decoction is a traditional Chinese recipe, and is composed of licorice, floating wheat, and jujube.MethodsEffects of lactic acid bacteria fermentation on the physicochemical properties, antioxidant activity, and γ-aminobutyric acid of Ganmai Dazao Decoction were studied. The changes of small and medium molecules in Ganmai Dazao Decoction before and after fermentation were determined by LC–MS non-targeted metabolomics.ResultsThe results showed that the contents of lactic acid, citric acid, acetic acid, and total phenol content increased significantly, DPPH free radical clearance and hydroxyl free radical clearance were significantly increased. γ-aminobutyric acid content was 12.06% higher after fermentation than before fermentation. A total of 553 differential metabolites were detected and identified from the Ganmai Dazao Decoction before and after fermentation by partial least squares discrimination and VIP analysis.DiscussionAmong the top 30 differential metabolites with VIP values, the content of five functional substances increased significantly. Our results showed that lactic acid bacteria fermentation of Ganmai Dazao Decoction improves its antioxidant effects and that fermentation of Ganmai Dazao Decoction with lactic acid bacteria is an innovative approach that improves the health-promoting ingredients of Ganmai Dazao Decoction.

IntroductionThis study explored the effects of Qigong exercises on upper extremity muscle activity, balance function, and quality of life in stroke patients.MethodsA total of 30 stroke patients were randomly allocated to either control group or Qigong group. In the Qigong group, participants completed an intervention of Qigong Baduanjin over 8 weeks. Data on the electromyographic activities of the biceps brachii muscle, triceps brachii muscle, and muscle coordination were obtained using surface electromyography and the co-contraction ratio (CCR). Data on balance were obtained using the PK254P balance function detection system. Quality of life was measured using the brief version of the World Health Organization Quality of Life scale.ResultsThe results for the Qigong group showed a significant difference in CCR of the triceps brachii muscle (p

The ancient traditional Chinese drink Bian-Que Triple-Bean Soup made by fermentation (FTBS) of Lactococcus lactis subsp. lactis YM313 and Lacticaseibacillus casei YQ336 is a potential functional drink. The effect of fermentation on the flavor and biological activity of FTBS was evaluated by analyzing its chemical composition. Five volatile flavors were detected in modified FTBS. Fermentation decreased the proportion of nonanal (beany flavor substances) but significantly increased the total flavone contents, phenol contents and many bioactive small molecule substances in FTBS. The changes of these substances led to the significant improvement of FTBS sensory evaluation, antioxidant activity and prebiotic potential. This research provides a theoretical basis for the application of Lactic acid bacteria (LAB) in the fermentation of edible plant-based foods and transformation from traditional food to industrial production.

Solid rocket motors (SRMs) have been popularly used in the current aerospace industry. Performance indicators, such as pressure and thrust, are of great importance for rocket monitoring and design. However, the measurement of such signals requires high economic and time costs. In many practical situations, the thrust measurement error is large and requires manual correction. In order to address this challenging problem, a lightweight RepVGG-based cross-modality data prediction method is proposed for SRMs. An end-to-end data prediction framework is established by transforming data across different modalities. A novel RepVGG deep neural network architecture is built, which is able to automatically learn features from raw data and predict new time-series data of different modalities. The effectiveness of the proposed method is extensively validated with the field SRM data. The accurate prediction of the thrust data can be achieved by exploring the pressure data. After calculation, the percentage error between the predicted data and the actual data is less than 5%. The proposed method offers a promising tool for cross-modality data prediction in real aerospace industries for SRMs.

BackgroundBrucellosis is a critical zoonotic disease in the world, it is the non-specific arthralgia that make brucellosis patients easily misdiagnosed as rheumatoid arthritis (RA) in endemic regions. Elevated rheumatoid factor (RF) is an essential indicator of RA, and the RF in brucellosis patients is significantly higher than healthy people. Therefore, this study further explored the distribution of RF and the relevant factors of the RF positivity in brucellosis patients with arthralgia, in order to strengthen the recognition of physicians for brucellosis patients with RF positivity, especially in brucellosis-endemic areas, so as to avoid misdiagnosis and untimely treatment that may lead to malignant outcomes.Methodology and principal findingsThe medical records of all 572 brucellosis inpatients were collected in the Sixth People's Hospital of Shenyang, China from 2015 to 2016. After excluding 106 patients without arthralgia, 5 patients who unwilling to perform RF testing and 16 patients with diseases that may affect RF, 445 brucellosis inpatients with arthralgia were involved in this retrospective cross-sectional study. 143 (32.1%) patients with RF >10 IU/ml were classified into the RF positive group, with an average level of 16.5[12.2, 34.7] IU/ml, of which 45 (10.1%) patients were high-positive with RF >30 IU/ml. Multivariate logistic regression model was used to further analyze the relevant factors of the RF positivity and found that age, wrist joint pain and elevated C-reactive protein (CRP) were positively associated with RF positivity, with OR of 1.02 (P = 0.024), 8.94 (P = 0.008) and 1.79 (P = 0.019), respectively.ConclusionThe prevalence of positive RF in brucellosis patients with arthralgia was critical, nearly one-third of patients had RF positive. Elderly men brucellosis patients with arthralgia, wrist joint pain and elevated CRP were at high risk of positive RF. It is reminded that physicians should focus on differential diagnosis during clinical diagnosis and treatment, especially in brucellosis-endemic regions.

Abstract Background To investigate the risk factors for brucellosis in suspected cases of the disease. Methods A self-designed questionnaire was developed to collect data from 3557 people whose initial visit site was the Songyuan Center for Disease Control and Prevention (CDC) from January 1st, 2009 to December 31st, 2012. After collecting blood samples, a plate agglutination test (PAT) and serum agglutination test (SAT) were used to distinguish the patients with brucellosis from the suspected cases. Results Sex, occupation (farmers and herdsmen), contact with abortion products, and contact with feces were the main risk factors for brucellosis in the suspected cases (all P

Two non-covalently linked copies of the retrovirus genome are specifically recruited to the site of virus particle assembly and packaged into released particles. Retroviral RNA packaging requires RNA export of the unspliced genomic RNA from the nucleus, translocation of the genome to virus assembly sites, and specific interaction with Gag, the main viral structural protein. While some aspects of the RNA packaging process are understood, many others remain poorly understood. In this review, we provide an update on recent advancements in understanding the mechanism of RNA packaging for retroviruses that cause disease in humans, i.e., HIV-1, HIV-2, and HTLV-1, as well as advances in the understanding of the details of genomic RNA nuclear export, genome translocation to virus assembly sites, and genomic RNA dimerization.

ObjectivesThe present study proposes a model for the relationships among competitive martial arts players’ positive emotions, hope (path and emotive force ideas), sense of control, self-handicapping, and precompetition state anxiety (PCSA). The model seeks to advance our understanding around the causal and mediating effects between these variables and, thus, has important implications for theory and practice in the field of sports and exercise psychology.MethodA total of 327 participants (male: 255, female: 72; age: 21.45 ± 2.78 years; athletic training: 6.27 ± 2.54 years) in the China University Wushu Sanda Championship 2019 were surveyed. Within 2 h before the match, PCSA, sports hope, sense of control, anxiety, and self-handicapping were measured.ResultsThe findings of the present study showed that (1) positive emotions have a significant positive correlation with path and emotive force ideas, anxiety orientation, ability to cope, goal attainment, and sense of control; (2) path and emotive force ideas were significantly positively correlated with anxiety orientation, ability to cope, goal attainment, and sense of control and significantly negatively correlated with anxiety intensity and self-handicapping; and (3) the path idea acted as a partial negative mediator between positive emotion and anxiety intensity, and the sense of control played a partial negative mediator between the emotive force idea and self-handicapping.Conclusion(1) Players’ positive emotions can predict the sense of hope. It also tends to interpret the anxiety intensity as a positive challenge rather than a negative sense of control. (2) Players with a higher path idea also tend to have lower negative emotion, anxiety intensity, and self-handicapping; (3) martial arts players’ path idea has a significantly higher predictive power for the sense of control than the emotive force idea. Still, both path and emotive force ideas can indirectly affect the intensity of anxiety orientation and self-handicapping through the multiple mediators of sense of control. Finally, recommendations for coaches related to training and preparation for competition are discussed.

Mammalian coat color is determined by heritable variations such as disease, nutrition, and hormone levels. Variation in animal coat color is also considered an environmental indicator and provides clues for the study of population genetics and biogeography. Records of abnormal coloration in the wild are rare, not only because it is often selected against, but also because of the difficulties in detection of the phenomenon. We used long-term camera-trapping data to first report abnormal coat coloration in yellow-throated marten (Martes flavigula) in China. Six types of abnormal coloration were found only in the Northeast Tiger and Leopard National Park, Northeast China, which were not reported in other regions in China. A total of 268 videos of Martes flavigula contained normal coloration, 455 videos of individuals of the species contained abnormal coloration, 437 contained the ‘gloving’ type (martens with de-pigmented front toes, paws or lower forelimbs), while the remaining other 18 videos contained five types (different degrees of white-spotting and dilution). The higher relative abundance index (0.428, ‘gloving’ to 0.329, normal) and wide distribution area of the ‘gloving’ type indicated that this abnormal coat coloration type is usual in Northeast China, which may reflect genetic variability in the local population. These records will contribute to further research on animal coat color and its corresponding adaptive strategy.

Previously, we found that the genotype of 42 out of 198 mouse microsatellite loci, which are distributed among all chromosomes except the Y chromosome, changed from monomorphism to polymorphism (CMP) in a genetically modified inbred mouse strain. In this study, we further examined whether CMP also relates to the homologous recombination in gene knockout (KO) mouse strains. The same 42 microsatellite loci were analyzed by polymerase chain reaction (PCR) in 29 KO inbred mouse strains via short tandem sequence repeat (STR) scanning and direct sequence cloning to justify microsatellite polymorphisms. The C57BL/6J and 129 mouse strains, from which these 29 KO mice were derived, were chosen as the background controls. The results indicated that 10 out of 42 (23.8%) loci showed CMP in some of these mouse strains. Except for the trinucleotide repeat locus of D3Mit22, which had microsatellite CMP in strain number 9, the core sequences of the remaining 41 loci were dinucleotide repeats, and 9 out of 41 (21.95%) showed CMPs among detected mouse strains. However, 11 out of 29 (37.9%) KO mice strains were recognized as having CMPs. The popular dinucleotide motifs in CMP were (TG)(n) (50%, 2/4), followed by (GT)(n) (27.27%, 3/11) and (CA)(n) (23.08%, 3/13). The microsatellite CMP in (CT)(n) and (AG)(n) repeats were 20% (1/5). According to cloning sequencing results, 6 KO mouse strains showed insertions of nucleotides whereas 1 showed a deletion. Furthermore, 2 loci (D13Mit3 and D14Mit102) revealed CMP in 2 strains, and mouse strain number 9 showed CMPs in two loci (D3Mit22 and D13Mit3) simultaneously. Collectively, these results indicated that microsatellite polymorphisms were present in the examined inbred KO mice.