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Highlights: [•] Saliva adsorption onto different materials was studied by means of QCM-D. [•] Film thickness was lowest for hydroxyapatite surfaces. [•] Viscoelastic properties depend on the substrate and saliva type. [•] SDS elution suggests different interaction mechanisms depending on the surface. [Copyright &y& Elsevier]

Abstract: Mucoadhesive buccal drug delivery systems can enhance rapid drug absorption by providing an increased retention time at the site of absorption and a steep concentration gradient. An understanding of the mechanisms behind mucoadhesion of polymers, e.g. chitosan, is necessary for improving the mucoadhesiveness of buccal formulations. The interaction between chitosan of different chain lengths and porcine gastric mucin (PGM) was studied using a complex coacervation model (CCM), isothermal titration calorimetry (ITC) and a tensile detachment model (TDM). The effect of pH was assessed in all three models and the approach to add a buffer to chitosan based drug delivery systems is a means to optimize and enhance buccal drug absorption. The CCM demonstrated optimal interactions between chitosan and PGM at pH 5.2. The ITC experiments showed a significantly increase in affinity between chitosan and PGM at pH 5.2 compared to pH 6.3 and that the interactions were entropy driven. The TDM showed a significantly increase in strength of adhesion between chitosan discs and an artificial mucosal surface at pH 5.2 compared to pH 6.8, addition of PGM increased the total work of adhesion by a factor of 10 as compared to the wetted surface without PGM. These findings suggest that chitosan and PGM are able to interact by electrostatic interactions and by improving the conditions for electrostatic interactions, the adhesion between chitosan and PGM becomes stronger. Also, the three complementary methods were utilized to conclude the pH dependency on mucoadhesiveness. [Copyright &y& Elsevier]

Highlights: [•] Tea polyphenols interaction with pre-adsorbed salivary proteins at the air–water interface is studied. [•] Surface dilation vicoelasticity of diluted saliva before and after exposure to tea polyphenol solutions is presented. [•] Results at the water air interface are discussed within a general mixed kinetic model framework. [•] The model correctly reproduces the experimental data except for complex galloylated theaflavins. [•] This work could help elucidate the mechanism behind tea astringency perception when coupled with a dedicated sensory study. [Copyright &y& Elsevier]

Highlights: [•] Interfacial properties of human whole saliva (HWS) are studied at the air–water interface. [•] Diluted HWS films build an elastic character with time due to adsorption/reorganisation of many salivary proteins. [•] As adsorption progresses the film nature changes from a soluble surfactant-like to an insoluble protein-like behaviour. [•] The surface dilation response to frequency is interpreted using a general mixed kinetic model. [Copyright &y& Elsevier]