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In this paper, we report the green synthesis of gold nanoparticles (AuNPs) using Wright tinctoria seed extract. The biomolecules present in the seed extract is responsible for the formation of AuNPs and they found to play dual role of both reducing as well as capping agents. Appearance of violet colour confirms the AuNPs formation. The surface nature of the synthesised nanoparticle is studied using SEM and TEM analysis. The high crystallinity of AuNPs is observed from the diffraction peaks in XRD profile. FTIR spectrum confirms the presence of different functional groups of biomolecules participated in encapsulating AuNPs. In this work, a possible mechanism of AuNPs formation was also proposed. The synthesised biogenic nano-scale gold particles by natural tribes are tested for its inborn antimicrobial activity. Antimicrobial activity of AuNPs showed better inhibitory activity towards certain pathogens in comparison to other test pathogens. [ABSTRACT FROM AUTHOR]