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Abstract The genomes of human gut bacteria in the genus Bacteroides include numerous operons for biosynthesis of diverse capsular polysaccharides (CPSs). The first two genes of each CPS operon encode a locus-specific paralog of transcription elongation factor NusG (called UpxY), which enhances transcript elongation, and a UpxZ protein that inhibits noncognate UpxYs. This process, together with promoter inversions, ensures that a single CPS operon is transcribed in most cells. Here, we use in-vivo nascent-RNA sequencing and promoter-less in-vitro transcription (PIVoT) to show that UpxY recognizes a paused RNA polymerase via sequences in both the exposed non-template DNA and the upstream duplex DNA. UpxY association is aided by ‘pause-then-escape’ nascent RNA hairpins. UpxZ binds non-cognate UpxYs to directly inhibit UpxY association. This UpxY-UpxZ hierarchical regulatory program allows Bacteroides to generate subpopulations of cells producing diverse CPSs for optimal fitness.

Abstract Cholesterol-dependent cytolysins (CDCs) comprise a large family of pore-forming toxins produced by Gram-positive bacteria, which are used to attack eukaryotic cells. Here, we functionally characterize a family of 2-component CDC-like (CDCL) toxins produced by the Gram-negative Bacteroidota that form pores by a mechanism only described for the mammalian complement membrane attack complex (MAC). We further show that the Bacteroides CDCLs are not eukaryotic cell toxins like the CDCs, but instead bind to and are proteolytically activated on the surface of closely related species, resulting in pore formation and cell death. The CDCL-producing Bacteroides is protected from the effects of its own CDCL by the presence of a surface lipoprotein that blocks CDCL pore formation. These studies suggest a prevalent mode of bacterial antagonism by a family of two-component CDCLs that function like mammalian MAC and that are wide-spread in the gut microbiota of diverse human populations.

Introduction: For venous thrombosis patients, catheter-directed thrombolytic therapy is the standard-of-care to recanalize the occluded vessel. Limitations with thrombolytic drugs make the development of adjuvant treatments an active area of research. One potential adjuvant is histotripsy, a focused ultrasound therapy that lyses red blood cells within thrombus via the spontaneous generation of bubbles. Histotripsy has also been shown to improve the efficacy of thrombolytic drugs, though the precise mechanism of enhancement has not been elucidated. In this study, in silico calculations were performed to determine the contribution of histotripsy-induced changes in thrombus diffusivity to alter catheter-directed therapy.Methods: An established and validated Monte Carlo calculation was used to predict the extent of histotripsy bubble activity. The distribution of thrombolytic drug was computed with a finite-difference time domain (FDTD) solution of the perfusion-diffusion equation. The FDTD calculation included changes in thrombus diffusivity based on outcomes of the Monte Carlo calculation. Fibrin degradation was determined using the known reaction rate of thrombolytic drug.Results: In the absence of histotripsy, thrombolytic delivery was restricted in close proximity to the catheter. Thrombolytic perfused throughout the focal region for calculations that included the effects of histotripsy, resulting in an increased degree of fibrinolysis.Discussion: These results were consistent with the outcomes of in vitro studies, suggesting histotripsy-induced changes in the thrombus diffusivity are a primary mechanism for enhancement of thrombolytic drugs.

Objective During the first wave of the SARS-CoV-2 pandemic, management of anticoagulation therapy in hospitalized patients with atrial fibrillation (AF) was simplified to low-molecular-weight heparin (LMWH) followed by oral anticoagulation, mainly owing to the risk of drug–drug interactions. However, not all oral anticoagulants carry the same risk. Methods Observational, retrospective, and multicenter study that consecutively included hospitalized patients with AF anticoagulated with LMWH followed by oral anticoagulation or edoxaban concomitantly with empirical COVID-19 therapy. Time-to-event (mortality, total bleeds, and admissions to ICU) curves, using an unadjusted Kaplan-Meier method and Cox regression model adjusted for potential confounders were constructed. Results A total of 232 patients were included (80.3 ± 7.7 years, 50.0% men, CHA 2 DS 2 -VASc 4.1 ± 1.4; HAS-BLED 2.6 ± 1.0). During hospitalization, patients were taking azithromycin (98.7%), hydroxychloroquine (89.7%), and ritonavir/lopinavir (81.5%). The mean length of hospital stay was 14.6 ± 7.2 days, and total follow-up was 31.6 ± 13.4 days; 12.9% of patients required admission to ICU, 18.5% died, and 9.9% had a bleeding complication (34.8% major bleeding). Length of hospital stay was longer in patients taking LMWH (16.0 ± 7.7 vs 13.3 ± 6.5 days; P = .005), but mortality and total bleeds were similar in patients treated with edoxaban and those treated with LMWH followed by oral anticoagulation. Conclusions Mortality rates, arterial and venous thromboembolic complications, and bleeds did not significantly differ between AF patients receiving anticoagulation therapy with edoxaban or LMWH followed by oral anticoagulation. However, the duration of hospitalization was significantly lower with edoxaban. Edoxaban had a similar therapeutic profile to LMWH followed by oral anticoagulation and may provide additional benefits.

ABSTRACT Purpose: to measure the total time of mastication and oral phase of swallowing in healthy elderly people, considering different food consistency, the presence of natural teeth, and the type of prosthetic oral rehabilitation, as well to compare dental conditions and consistencies. Methods: a cross-sectional study with 57 healthy elderly people, aged between 60 and 82 years, divided into: dentate group (D) (n=15); and rehabilitated groups; PD: fixed or removable partial dentures in one or both arches (n=15); CRD: maxillary and mandibular complete removable dentures (n=15); ISCD: complete dentures - maxillary removable denture and mandibular implant-supported dentures (n=12). The duration time (seconds) of chewing and of the oral phase of swallowing was measured in the consistencies: solid (a roll slice), pudding (10 ml) and liquid (10 ml). Results: healthy dentate elderly subjects had the following intervals: liquid=1.07±0.35 s; pudding=3.48±1.76 s; and solid=27.88±7.11 s. Statistically significant differences were observed between the D and ISCD groups (p=0.0056) and between D and CRD (p=0.0056) for liquid. For all groups, there was a statistically significant increase between liquid and solid consistencies and between pasty and solid ones (p

RESUMO Objetivo Apresentar Exame Clínico Miofuncional Orofacial pertencente ao Protocolo MMBGR - Lactentes e Pré-escolares, incluindo sua validação. Método Inicialmente foi realizada a validade do conteúdo do teste adaptado do Protocolo MBGR, para faixa etária entre 6 e 71 meses, fundamentada na bibliografia e experiência entre autores (originais e atuais). Para análise de conteúdo e aparência participaram 10 fonoaudiólogos especialistas em Motricidade Orofacial, que preencheram formulário eletrônico com questões dicóticas e escala de Likert, em dois momentos. Foi calculado Índice de Validade de Conteúdo e Teste Binomial Exato. Na sequência houve análise da validade baseada nos processos de resposta, seguida da análise da confiabilidade do Exame Clínico, com 155 participantes, por 7 fonoaudiólogos experientes e calibrados, sendo verificada a concordância entre e intra examinadores pelo Coeficiente de Correlação Intraclasse. Resultados Houve acréscimos, modificações e exclusão de itens conforme faixa etária, concluindo-se o Protocolo Exame Clínico Miofuncional Orofacial para lactentes e pré-escolares, que obteve 90,5% com concordância; e 100% dos escores adequados por pelo menos 90% dos especialistas. Quanto à confiabilidade, a maioria dos itens dos Exames Extraoral e Intraoral e Mastigação obtiveram concordância razoável a boa, ou, até mesmo, excelente. Conclusão O “Exame Clínico Miofuncional Orofacial” teve validação baseada no conteúdo do teste, nos processos de resposta e confiabilidade concluída, e junto ao “Instrutivo” e à “História Clínica” integra o “Protocolo MMBGR - Lactentes e Pré-escolares”, para atuação fonoaudiológica na faixa etária entre 6 e 71 meses de idade.

RESUMO Objetivo Apresentar Instrutivo e Protocolo de História Clínica Miofuncional Orofacial pertencentes ao Protocolo MMBGR - Lactentes e Pré-escolares, incluindo adaptação e validação do conteúdo e aparência destes. Método Estudo tipo validação, descritivo e transversal. Adaptação a partir do Protocolo MBGR, fundamentada em estudos teóricos e experiência dos autores. Considerados lactentes entre 6 e 23 meses de vida e pré-escolares entre 24 e 71 meses. Obtido consentimento e consenso da versão adaptada pelos autores (originais e atuais). Analisados aparência e conteúdo do novo instrumento por 10 fonoaudiólogos especialistas em Motricidade Orofacial. Realizadas duas rodadas de análise: Primeira com formulário eletrônico contendo questões dicóticas (sim/não), com espaço para justificar as respostas negativas; calculado Índice de Validade de Conteúdo e Teste Binomial Exato; e Segunda com escala Likert 5 posições. Resultados Produzido instrutivo inédito e adaptado o Protocolo de História Clínica, com manutenção de 23 itens pertinentes à faixa etária em questão. Excluídas informações em 7 itens e acrescidas em 8 itens. Inicialmente obteve-se concordâncias de 70% do instrutivo, por pelo menos 70% dos especialistas; e 64% dos itens da História Clínica, por ao menos 90% dos especialistas. Obteve-se na segunda rodada 100% de respostas “Concordo totalmente” dos especialistas. Conclusão “Instrutivo” e “História Clínica Miofuncional Orofacial” tiveram validade de conteúdo e aparência concluída, e junto ao “Exame Clínico” integram o “Protocolo MMBGR - Lactentes e Pré-escolares”, com potencial contribuição para atuação clínica e na pesquisa em Motricidade Orofacial na faixa etária de 6 meses a 5 anos e 11 meses de idade.

RESUMO Objetivo Investigar a autopercepção do ponto de constrição da língua na produção de [s] e [z] por jovens monolíngues com posicionamento de língua normal e alterado. Método Três fonoaudiólogas analisaram gravações de fala em vídeo de 49 mulheres, com idades entre 18 e 28 anos (média=20 anos e 7 meses), classificando-as em: Grupo 1, (G1, n=25), com ausência de alterações no posicionamento de língua na produção de [s] e [z] e Grupo 2 (G2, n=24), com alterações no posicionamento de língua nestes fones. A autopercepção do ponto de constrição da língua foi investigada questionando cada jovem sobre o local em que sua ponta da língua encostava (apical, laminal ou “outros ajustes”) ao produzir [s] e [z] na leitura de palavras e pseudopalavras. O teste de Friedman, com comparações par a par posteriores, foi utilizado para análise intragrupo. O teste de Mann-Whitney foi utilizado para as comparações entre grupos. Adotou-se nível de significância de 5%. Resultados Em G1 houve relatos de ponto de constrição apical e laminal enquanto em G2 houve estes relatos, e também de outros ajustes de língua. A presença de outros ajustes da língua diferenciou G1 e G2 (p=0,002). Em G1, houve diferença significativa entre [s] e [z], sendo que o ponto laminal ocorreu com mais frequência em [s] do que em [z]. Conclusão Jovens com posicionamento de língua normal e alterado relataram pontos de constrição da língua apical e laminal em fricativas alveolares. Porém, outros ajustes de língua foram percebidos diante de posicionamento de língua alterado.

RESUMO Objetivo Este estudo visou validar o Protocolo de Avaliação Miofuncional Orofacial MBGR para adultos com DTM. Método Participaram 30 adultos, sendo 15 com DTM (deslocamento de disco com redução segundo o Research Diagnostic Criteria for Temporomandibular Disorders) e 15 Controles. O processo de validação envolveu a validade de conteúdo, de critério e de construto, além da concordância inter e intra-avaliador, sensibilidade e especificidade. Considerou-se nível de significância de 5%. Resultados Foram confirmadas as validades de conteúdo, visto que o Protocolo MBGR abarca todas as questões funcionais presentes em indivíduos com DTM; de critério, com correlações significantes entre o MBGR e AMIOFE; e de construto, diferenciando indivíduos com e sem DMO quanto à dor à palpação e mobilidade mandibular, com correlação significante entre a avaliação clínica do MBGR e o uso do algômetro digital, bem como confirmação do exame instrumental para a classificação do modo respiratório. A força de concordância variou de pobre a muito boa para a análise interavaliador e de razoável a muito boa para intra-avaliador. Os valores de sensibilidade e especificidade foram elevados. Conclusão O Protocolo MBGR mostrou-se válido para aplicação em adultos com DTM com deslocamento de disco com redução e controles, contemplando todos os aspectos que possibilitam a análise das condições oromiofuncionais nesses indivíduos.

Abstract The tongue participates in the oral phase of swallowing by pushing the food bolus toward the oropharynx. This relationship between tongue function and swallowing is little addressed addressed in individuals with temporomandibular dysfunction (TMD). Objective: To analyze the association of functional tongue conditions on swallowing in individuals with TMD. Methodology: After approval by the Institutional Review Board, the study was conducted on 30 individuals of both sexes, aged 18 to 28 years, with TMD, and not treated for the disorder. Tongue function was assessed as to the mobility, pressure, and oral motor control. Swallowing was analyzed by clinical assessment during ingestion of solid (wafer biscuit) and liquid (water). Data regarding mobility and swallowing were collected using the orofacial myofunctional evaluation protocol. Tongue pressure was measured by the Iowa Oral Performance Instrument, during elevation, protrusion, swallowing, and resistance test. The oral motor control was assessed by the oral diadochokinesis (DDK) test by rapid and repeated emissions of syllables “ta” and “ka”. Data were statistically analyzed by the Spearman correlation coefficient, at a significance level of 5%. Results: Relationships were found between tongue function and swallowing for the following aspects: mobility (r=0.741), pressure in protrusion (r=-0.366), swallowing of saliva (r=-0.499), mean DDK rate in emissions “ta” (r=-0.424) and “ka” (r=-0.446), and mean DDK period in emissions “ta” (r=0.424) and “ka” (r=0.446). Thus, the greater the change in tongue mobility, the lower the tongue pressure in protrusion and swallowing of saliva, the lower the emissions per second, the longer the mean time between vocalizations, and the worse the swallowing of individuals with TMD. Conclusion: The functional conditions of the tongue regarding mobility, pressure, and oral DDK were associated with swallowing in individuals with TMD.

RESUMO Objetivo Realizar a ampliação, validação de conteúdo, critério e construto de um protocolo de avaliação miofuncional orofacial para indivíduos com fissura labiopalatina e definir parâmetros de avaliação para a utilização do instrumento. Método A ampliação do instrumento foi realizada com base no protocolo MBGR; os itens, subitens e possibilidades de respostas do instrumento ampliado foram analisados quanto à clareza por sete examinadores para a validação do conteúdo. Parâmetros de avaliação para a utilização do instrumento foram estabelecidos a fim de minimizar a subjetividade. Quatro examinadores compararam os aspectos do protocolo aos de outro instrumento para a validação de critério, e a validação de construto foi realizada comparando os resultados pré e pós-tratamento cirúrgico do avanço da maxila. Aplicou-se o Índice de Validação do Conteúdo e os testes Kappa, Correlação de Spearman e Wilcoxon para as validações. Resultados Foram acrescentados os aspectos da avaliação da mobilidade, tonicidade e sensibilidade ao protocolo; na validação do conteúdo, 72% dos itens foram classificados como muito claro e 28%, como claro; construiu-se um manual com parâmetros de avaliação para todos os itens e subitens do protocolo. A concordância interexaminadores apresentou-se moderada. Na comparação dos protocolos, para a validação de critério, foi observada boa relação entre eles. Para a validação do construto, o protocolo demonstrou ser capaz de identificar resultado de tratamento após a realização do tratamento. Conclusão O protocolo foi ampliado e considerado validado em sua totalidade: conteúdo, critério e construto, e estabelecidos parâmetros de avaliação para a sua utilização.

ABSTRACT Purpose: to identify the focus of scientific publications in the field of orofacial motricity in individuals with cleft lip and palate, as well as validated protocols used in speech and language evaluation. Methods: a study conducted using the following databases: Medline, SciELO, Lilacs and Google Scholar, through keywords including: cleft palate + cleft lip + evaluation + speech therapy + stomatognathic system + speech + phonation + chewing + swallowing + breathing + validation studies, in Portuguese and English. Results: a total of 572 articles that addressed the evaluation of the stomatognathic system was selected. The articles were published between 2012 and 2017, and examined for year of publication, subject area, focus, instrument used for evaluation, and type of study. Of these, 90 articles met the inclusion criteria, but only 5 used validated protocols. The greatest number of articles was published in 2014. The studies covered broad age groups, using subjective and objective methods, and instruments were often non-standardized. Conclusion: speech was the prevalent theme, while other orofacial functions were poorly investigated, demonstrating that studies with other approaches were lacking.

Abstract OBJECTIVE To assesses the quality of life and the impact of oral health conditions in the pre- and post-surgical correction of dentofacial deformity of individuals with cleft lip and palate. METHOD Quantitative and prospective study in a referral hospital in the period from June 2011 to May 2013. A total of 50 participants responded the questionnaires World Health Organization Quality of Life - Bref (WHOQOL-Bref) and Oral Health Impact Profile - 14 (OHIP-14), approximately 3 days before and 3 to 12 months after surgery. The "t" test for paired samples and Wilcoxon test were used for statistical analysis with a significance level of 5%. RESULTS After surgery, differences were found in the domains: Physical, Psychological, Environmental and General Questions of the WHOQOL-Bref and domains: Psychological Discomfort, Psychological Disability, Social Disability, Handicap and Overall Score of OHIP-14. CONCLUSION Surgical correction of dentofacial deformity improved quality of life and had positive impact of oral health condition.

OBJETIVO: devido à presença de importante disfunção mastigatória em casos com anquilose da articulação temporomandibular (ATM), este trabalho analisou a abertura bucal e a atividade eletromiográfica dos músculos mastigatórios para detectar mudanças nesses parâmetros após a cirurgia de liberação da anquilose mandibular. MÉTODO: em 7 indivíduos com anquilose temporomandibular, entre 7 e 30 anos (mediana=9 anos), foi mensurada a distância interincisal máxima ativa (DIMA) e registrada a atividade eletromiográfica (EMG) dos músculos masseteres e temporais durante a contração voluntária isométrica máxima (CVIM) e a mastigação habitual, comparando-se os dados pré e pós cirúrgicos pelo teste de Wilcoxon. RESULTADOS: maiores valores foram observados para a DIMA após a cirurgia (p=0,0277); o índice de assimetria, não mostrou diferença entre os dois momentos avaliados para ambos os músculos estudados; os valores da EMG durante a CVIM diminuíram após a cirurgia para os músculos masseter direito (p=0,0179) e esquerdo (p=0,0179), mas não para os temporais; não houve modificação nos valores EMG para os músculos estudados, durante a mastigação. CONCLUSÃO: a cirurgia para liberação da anquilose da ATM resultou em aumento da abertura bucal e diminuição da amplitude dos potenciais de ação gerados durante a contração voluntária isométrica máxima para o músculo masseter, em ambos os lados; não modificou o índice de assimetria dos músculos masseter e temporal, bem como a atividade eletromiográfica do músculo temporal, bilateralmente, durante a contração isométrica máxima e músculos masseter e temporal durante a mastigação habitual.PURPOSE: due to the presence of major masticatory dysfunction in patients with temporomandibular joint (TMJ) ankylosis, this study analyzed mouth opening and EMG activity of masticatory muscles in order to detect changes in these parameters after surgical release of mandible ankylosis. METHOD: in 7 patients with temporomandibular ankylosis, between 7 and 30 years (median = 9 years), the distance was measured as interincisal maximum active (DIMA) and we recorded the electromyographic activity (EMG) of masseter and temporal muscles during voluntary isometric contraction (VIC) and chewing, comparing the data before and after surgery using the Wilcoxon test. RESULTS: higher values were observed for DIMA after surgery (p=0.0277), the asymmetry index showed no difference between the two evaluated periods for both studied muscles, the values of the EMG during VIC decreased after surgery for the right (p=0.0179) and left (p=0.0179) masseter but not for the temporal muscle, there were no changes in EMG values for the studied muscles during mastication. CONCLUSION: the surgical release of TMJ ankylosis resulted in an increase of mouth opening and decreased amplitude of action potentials generated during maximum isometric voluntary contraction of the masseter muscle on both sides, this did not change the asymmetry index of the masseter and temporal as well as the electromyographic activity of the temporal muscle bilaterally during isometric contraction and masseter and temporal muscles during mastication.

OBJETIVO: apresentar um protocolo com escores na área de motricidade orofacial intitulado Protocolo MBGR. MÉTODOS: a partir de protocolos utilizados na área da motricidade orofacial foi elaborado um novo protocolo contendo escores, o qual foi aplicado em sujeitos normais e em pacientes, em cinco fases distintas durante dois anos. A aplicação do protocolo, em cada fase, foi realizada por juízes independentes. Sempre ao final de cada fase o grupo que elaborou o protocolo fez os ajustes necessários para que o mesmo pudesse ser sensível aos problemas da área da motricidade orofacial. RESULTADOS: um novo protocolo na área de motricidade orofacial com escores foi elaborado e testado durante dois anos em cinco diferentes fases possibilitando obter parâmetros numéricos. CONCLUSÃO: foi apresentado o Protocolo MBGR com escores, o qual permite ao fonoaudiólogo avaliar, diagnosticar e estabelecer prognóstico em motricidade orofacial.PURPOSE: to present a protocol with scores on the Orofacial Myofunctional area. This protocol is called MBGR. METHODS: based on different protocols used in the Orofacial Myofunctional area, a new protocol with scores was elaborated. This protocol was applied in both, patients and non-patients during five distinct phases during the elaboration. After each phase, the results were analysed and the necessary corrections were performed in order to improve the protocol and, in this way, provide an accurate diagnosis and prognosis. It took two years to elaborate the protocol. During those two years, independent judges were responsible for testing the protocol in each phase. RESULTS: a new protocol with scores on the Orofacial Myofunctional area was elaborated and tested during two years in different phases. The new MBGR Protocol gives the possibility to obtain numeric parameters. CONCLUSION: the MBGR Protocol with scores was presented. This protocol helps the Speech-Language Pathologist to evaluate and give the diagnosis and the prognosis on the Orofacial Myology area.

Objetivo: elaborar um protocolo de avaliação clínica da função mastigatória, visando auxiliar o fonoaudiólogo a identificar e interpretar os sinais clínicos de alterações na dinâmica da mastigação, além de possibilitar comparações de acompanhamento de tratamento e entre diferentes avaliadores. MÉTODOS: este protocolo foi elaborado com base na prática clínica de fonoaudiólogos que responderam a um questionário enviado por e-mail; na literatura, segundo a identificação dos pontos comuns a todos os protocolos de avaliação da mastigação; além da experiência dos fonoaudiólogos do Hospital de Reabilitação de Anomalias Craniofaciais da Universidade de São Paulo. RESULTADOS: o protocolo de avaliação clínica da mastigação contempla aspectos relacionados aos aspectos morfológicos e à neurofisiologia da função mastigatória: CONCLUSÃO: este trabalho contribui com a Fonoaudiologia, uma vez que busca, de maneira mais completa possível, critérios para a avaliação da mastigação, norteando a atuação fonoaudiológica clínica baseada em evidências.PURPOSE: to elaborate a protocol for the clinical evaluation of the masticatory function, aiming at aiding the speech pathologist to identify and interpret the clinical signals of the masticatory disorders, in its dynamics, in addition to allowing for inter-judge comparison and treatment continuation. METHODS: this protocol was elaborated based on the clinical practice of the speech pathologists that answered to a questionnaire sent by email; in the literature, according to the identification of the common points to all chewing evaluation protocols; in addition to the experience of the speech pathologists of HRAC/USP. RESULTS: the protocol for the clinical chewing evaluation considers aspects related to morphologic aspects and neurophysiology of the masticatory function. Conclusion: this study contributes to Speech Pathology, once that it searches, in the most possible complete manner, criteria for evaluating the chewing, guiding the clinical speech-language performance based on evidences.

OBJETIVO: verificar a ocorrência de disfunção temporomandibular em crianças do município de Monte Negro/RO, correlacionando os achados à presença de hábitos orais deletérios. MÉTODOS: investigou-se a presença de quadros de disfunção temporomandibular, classificando-se em disfunção temporomandibular articular, muscular ou mista, bem como a presença de hábitos orais deletérios de sucção (mamadeira, chupeta e digital) e mastigação (onicofagia, morder objetos, morder a mucosa oral ou labial, bruxismo e apertamento dentário), em 79 crianças de seis a 11 anos (41 meninas e 38 meninos). RESULTADOS: dentre as 79 crianças investigadas, 27 (34,18%) apresentaram sinais de disfunção temporomandibular, sendo a disfunção articular a mais encontrada (48,15% n=13), seguida da mista (33,33%, n=9) e, com menor ocorrência, a muscular (18,52%, n=5 crianças). Hábitos orais deletérios foram encontrados em 69,62% (n=55) da amostra, sendo maior o número de crianças com hábitos de mastigação (39,91%, n=26) que de sucção (8,86%, n=7), observando-se casos em que os dois tipos de hábito estavam presentes (27,85%, n=22). Os resultados não demonstraram associação entre a presença de hábitos orais deletérios e disfunção temporomandibular (teste Qui-quadrado), independentemente do tipo de hábito. CONCLUSÃO: das crianças avaliadas, um terço (34%) apresentou sinais de disfunção temporomandibular, não havendo associação entre a disfunção temporomandibular com a presença de hábitos orais deletérios.PURPOSE: to check the occurrence of temporomandibular disorder in children from Monte Negro/RO, correlating the findings to the presence of harmful oral habits. METHODS: the study was conducted on 79 children aged form 6 to 11-year old (41 girls and 38 boys). The presence of temporomandibular disorder was investigated and classified into articular, muscular and mixed. The presence of harmful oral habits involving sucking (baby bottle, pacifier, finger/thumb sucking) and chewing (nail biting, biting objects, biting the oral or labial mucosa, bruxism and clenching) was also found. RESULTS: among the 79 investigated children, 27 (34.18%) showed symptoms of temporomandibular disorder, with highest periodicity of articular disorder (48.15%, n=13), followed by mixed (33.33%, n=9) and muscular disorder (18.52%, n=5). Harmful oral habits were found in 69.62% (n=55), mostly involving chewing habits (39.91%, n=26) followed by sucking (8.86%, n=7); some children showed both types of habits (27.85%, n=22). The results did not demonstrate any association among harmful oral habits and temporomandibular disorder according to the chi-square test, regardless of the type of habit (chewing or suction). CONCLUSION: one third of children evaluated (34%) presented symptoms of temporomandibular disorder, without any association between temporomandibular disorder and the presence of harmful oral habits.

Temporomandibular dysfunction (TMD) is a complex disturbance that involves the masticatory muscles and/or temporomandibular joint, causing damage to the masticatory function. This study evaluated the electromyographic activity of the masseter muscle during habitual mastication of bread, apple, banana, cashew nut and paraffin film (Parafilm M) in 25 adult subjects, of both gender, with TMD. The results were compared to those of a control group, composed of 15 adult subjects, of both sexes, free of signs and/or symptoms of TMD. The MYO-TRONICS Inc., K6-I computer software was used for electromyographic processing and analyzed the following parameters: duration of the act, duration of the masticatory cycle and number of cycles. No significant differences were found between subjects in the control group and individuals with TMD as to duration of the masticatory act and of the masticatory cycle, considering all materials used for mastication. The duration of the masticatory act and cycle was longer during mastication of paraffin film in both groups. The number of masticatory cycles was higher for mastication of apple in comparison to mastication of banana, in both groups. It can be concluded that the consistency of foods influences the duration parameters of the act, duration of the cycle and the number of masticatory cycles, and the behavior of the masticatory muscles in individuals with TMD during habitual mastication is similar to that verified in individuals without TMD.A disfunção temporomandibular (DTM) representa um quadro complexo que envolve os músculos mastigatórios e/ou a articulação temporomandibular, causando prejuízos à função mastigatória. Este estudo avaliou a atividade eletromiográfica do músculo masseter durante a mastigação habitual de pão, maçã, banana, castanha de caju e folha de parafilme (Parafilm M) em 25 indivíduos adultos, de ambos os gêneros, com DTM. Os resultados foram comparados com os obtidos para o grupo controle, composto por 15 indivíduos adultos, de ambos os gêneros, livres de sinais e/ou sintomas de DTM. Foi utilizado o programa computadorizado MYO-TRONICS Inc., K6-I no modo de processamento eletromiográfico, tendo sido analisados os seguintes parâmetros: duração do ato, duração do ciclo mastigatório e número de ciclos mastigatórios. Não foram encontradas diferenças estatisticamente significantes entre os indivíduos do grupo controle e com DTM no que diz respeito à duração do ato e do ciclo mastigatório, bem como o número de ciclos, considerando todos os materiais utlizados para a mastigação. A duração do ato e do ciclo mastigatório foi maior durante a mastigação de parafilme em ambos os grupos. O número de ciclos mastigatórios foi maior para a mastigação de maçã, em comparação à banana, nos diferentes grupos. Pode ser concluído que a consistência dos alimentos influencia a duração dos parâmetros duração do ato, duração do ciclo e número de ciclos mastigatórios e o comportamento dos músculos mastigatórios em indivíduos com DTM durante a mastigação habitual é semelhante à verificada em indivíduos sem disfunção.

The main purpose of this study was to investigate the impact of maxillary advancement on speech resonance in subjects with cleft lip and palate. The study sample was composed of 42 subjects aged 16 to 41 years old with operated cleft palate ± lip submitted to maxillary advancement. Resonance was evaluated before and 3 to 12 months after surgery by perceptual analysis and graduated from absent to severe. It was observed that 47.5% of the subjects presented impairment of resonance after orthognathic surgery, with a confidence interval (at 95%) from 31.5% to 63.9%. These results suggest that orthognathic surgery in individuals with cleft palate may interfere in resonance, causing, or increasing the degree of hypernasality. Therefore, this highlights the importance of the orientation about the risks and benefits of maxillary advancement surgery and follow-up of these patients.O propósito deste estudo foi verificar o impacto do avanço de maxila na ressonância de fala em sujeitos com fissura labiopalatina. A amostra do presente estudo foi composta por 42 sujeitos, entre 16 e 41 anos de idade, com fissura de palato associada ou não à de lábio submetidos ao avanço da maxila. A ressonância foi avaliada entre 3 e 12 meses após a cirurgia por análise e perceptual e graduada de ausente a severa. Observou-se que 47.5% dos sujeitos apresentaram prejuízo da ressonância após a cirurgia ortognática, com um intervalo de confiança (a 95%) de 31.5% a 63.9%. Esses resultados sugerem que a cirurgia ortognática, em sujeitos com fissura palatina, pode interferir na ressonância, causando ou aumentando o grau de hipernasalidade. Portanto, isso elucida a importância da orientação sobre os riscos e benefícios da cirurgia de avanço da maxila e acompanhamento desses pacientes.

OBJETIVO: devido à presença de importante disfunção mastigatória em casos com anquilose da articulação temporomandibular (ATM), este trabalho analisou a abertura bucal e a atividade eletromiográfica dos músculos mastigatórios para detectar mudanças nesses parâmetros após a cirurgia de liberação da anquilose mandibular. MÉTODO: em 7 indivíduos com anquilose temporomandibular, entre 7 e 30 anos (mediana=9 anos), foi mensurada a distância interincisal máxima ativa (DIMA) e registrada a atividade eletromiográfica (EMG) dos músculos masseteres e temporais durante a contração voluntária isométrica máxima (CVIM) e a mastigação habitual, comparando-se os dados pré e pós cirúrgicos pelo teste de Wilcoxon. RESULTADOS: maiores valores foram observados para a DIMA após a cirurgia (p=0,0277); o índice de assimetria, não mostrou diferença entre os dois momentos avaliados para ambos os músculos estudados; os valores da EMG durante a CVIM diminuíram após a cirurgia para os músculos masseter direito (p=0,0179) e esquerdo (p=0,0179), mas não para os temporais; não houve modificação nos valores EMG para os músculos estudados, durante a mastigação. CONCLUSÃO: a cirurgia para liberação da anquilose da ATM resultou em aumento da abertura bucal e diminuição da amplitude dos potenciais de ação gerados durante a contração voluntária isométrica máxima para o músculo masseter, em ambos os lados; não modificou o índice de assimetria dos músculos masseter e temporal, bem como a atividade eletromiográfica do músculo temporal, bilateralmente, durante a contração isométrica máxima e músculos masseter e temporal durante a mastigação habitual.PURPOSE: due to the presence of major masticatory dysfunction in patients with temporomandibular joint (TMJ) ankylosis, this study analyzed mouth opening and EMG activity of masticatory muscles in order to detect changes in these parameters after surgical release of mandible ankylosis. METHOD: in 7 patients with temporomandibular ankylosis, between 7 and 30 years (median = 9 years), the distance was measured as interincisal maximum active (DIMA) and we recorded the electromyographic activity (EMG) of masseter and temporal muscles during voluntary isometric contraction (VIC) and chewing, comparing the data before and after surgery using the Wilcoxon test. RESULTS: higher values were observed for DIMA after surgery (p=0.0277), the asymmetry index showed no difference between the two evaluated periods for both studied muscles, the values of the EMG during VIC decreased after surgery for the right (p=0.0179) and left (p=0.0179) masseter but not for the temporal muscle, there were no changes in EMG values for the studied muscles during mastication. CONCLUSION: the surgical release of TMJ ankylosis resulted in an increase of mouth opening and decreased amplitude of action potentials generated during maximum isometric voluntary contraction of the masseter muscle on both sides, this did not change the asymmetry index of the masseter and temporal as well as the electromyographic activity of the temporal muscle bilaterally during isometric contraction and masseter and temporal muscles during mastication.

OBJETIVO: Verificar a influência da idade sobre as dimensões nasofaríngeas e os sintomas respiratórios em pacientes submetidos à cirurgia para a correção da insuficiência velofaríngea pela técnica de retalho faríngeo. MÉTODOS: Amostra formada por 103 indivíduos distribuídos em três grupos: crianças (6 a 12 anos), adultos jovens (18 a 30 anos) e adultos de meia-idade (≥40 anos). Trata-se de estudo retrospectivo que analisou os valores da área nasofaríngea (técnica fluxo-pressão, valor de referência 0,570 cm²) e os sintomas de respiração oral, ronco e dificuldade respiratória durante sono, obtidos, em média, 12 meses após a cirurgia. A área nasofaríngea foi comparada entre os grupos por análise de variância e os sintomas respiratórios pelo teste Qui-quadrado (p

Purpose: To create and validate the content of an orofacial myofunctional assessment protocol for individuals with cleft lip and palate.Methods:The first version of an orofacial myofunctional assessment protocol for individuals with cleft lip and palate was created by two speech-language pathologists, who contemplated the structural and functional aspects of the stomatognathic system. This version was analyzed by other two speech-language pathologists experienced in cleft lip and palate assessment, who suggested changes that led to the second version of the protocol. Dynamic and static images necessary for performing the orofacial myofunctional examination were recorded from three individuals with cleft lip and palate, who represented three life stages: childhood, adolescence, and adulthood. Five examiners evaluated the images, applied the proposed protocol, and judged each item regarding its clarity to validate the content, from Content Validity Index.Results:The assessment protocol was finalized with 13 items, ten related to structural aspects and three related to functional aspects, with their corresponding sub-items. The general agreement in the validation of its content was 100%, so that only one stage was required.Conclusion:A protocol to evaluate the orofacial myofunctional aspects of individuals with cleft lip and palate was created with 13 items, as well as their corresponding sub-items, and its content was validated.

RESUMO Objetivo: Apresentar valores para a pressão da língua em adultos jovens brasileiros, considerando provas específicas e possíveis diferenças quanto ao gênero e tipo de prova. Método: Foram avaliados 51 voluntários de 18 a 28 anos, ambos os gêneros, com boa relação dento-oclusal e sem alterações de fala. Utilizou-se o Iowa Oral Performance Instrument na avaliação da pressão da língua (Kilopascal) durante as provas de elevação, protrusão, deglutição e lateralização, além do teste de resistência (segundos). Na análise dos resultados foi utilizado o ANOVA, seguido do teste de comparações múltiplas Tukey, adotando o nível de significância de 5%. Resultados: Os valores obtidos, respectivamente para homens e mulheres, na prova de elevação foram 63,94±12,92 e 50,27±15,29, na protrusão 60,22±13,62 e 44,30±12,95, na deglutição 33,94±12,06 e 34,27±13,25, na lateralização à direita 44,15±10,47 e 31,85±8,46, na lateralização à esquerda 43,15±10,22 e 29,55±8,91, e no teste de resistência 24,85±10,95 e 17,35±6,71. Os homens apresentaram valor maior nas provas de protrusão e de lateralização; os valores das provas de protrusão e elevação não diferiram entre si para o gênero masculino, mas foram maiores que a lateralização e a deglutição em ambos os gêneros; não houve diferença na prova de lateralização em relação ao lado em ambos os gêneros. Conclusão: Valores de pressão da língua em adultos jovens brasileiros foram determinados para provas específicas; o gênero influenciou nos valores das provas de protrusão e lateralização; os valores obtidos nas provas de elevação e protrusão foram maiores que na lateralização e deglutição.

Objetivo Correlacionar o desempenho motor oral nas tarefas de diadococinesia, de acordo com o tipo de fissura e com a presença de ceceio na fala, em crianças com fissura labiopalatina, operadas. Métodos Foram estudadas 30 crianças com fissura completa unilateral de lábio e palato e 30 com fissura isolada de palato, operadas, com idade entre 9 e 12 anos. Dois fonoaudiólogos analisaram a repetição de frases com os fones [s] e [z] para identificar a presença de ceceio e a análise da diadococinesia ocorreu a partir do registro da repetição das sílabas “ta” e “ca” e da sequência “pataca”. Na análise, o teste Kappa verificou a concordância entre os avaliadores, o teste Qui-quadrado a frequência de ceceio entre os grupos e o teste t os valores da diadococinesia, segundo o tipo de fissura e a presença de ceceio. Resultados O número de emissões por segundo e os tempos médios entre as emissões não demonstraram diferença significativa entre os grupos. Houve boa concordância entre os avaliadores quanto à presença de ceceio, com maior frequência nos casos com fissura completa unilateral de lábio e palato. A comparação entre os valores da diadococinesia e a presença de ceceio não evidenciou diferença significativa. Conclusão O desempenho nas tarefas da diadococinesia não sofreu alteração segundo o tipo de fissura labiopalatina. A presença de ceceio na fala foi maior no grupo com fissura completa unilateral de lábio e palato, sem modificação, porém, no desempenho das tarefas da diadococinesia.

RESUMO Objetivo: analisar as características estéticas da face em indivíduos com deformidades dentofaciais (DDF) e os possíveis aspectos que se relacionam com aspectos da pele quanto às rugas e sulcos. Métodos: avaliou-se 36 adultos jovens (média de 27,22 anos), em três grupos, DDF padrão II, III e grupo controle (GC). Realizou-se análise facial utilizando o Protocolo de Avaliação Miofuncional Orofacial MBGR. Elaborou-se um protocolo de avaliação estético facial a partir das análises validadas para a classificação de rugas, linhas de marionete e sulcos na região orbicular dos olhos. Aplicaram-se testes estatísticos pertinentes. Resultados: indivíduos com DDF e 50% do GC apresentaram alteração quanto à assimetria facial e ângulo nasolabial, com diferença estatisticamente significante entre DDF-II, DDF-III e GC. Indivíduos com DDF mostraram maior frequência de alteração para os sulcos verticais na região dos lábios e linhas de marionete, sem diferenças entre os grupos. Houve correlação positiva entre sulco na região do prócero e os sulcos verticais nos lábios, sulco na região do orbicular dos olhos com o sulco nasogeniano e com as linhas de marionete. O ângulo nasolabial correlacionou-se com sulco na região da testa. A idade correlacionou-se com sulco nasogeniano, sulco na região do prócero, linhas de marionete e sulcos na região do orbicular dos lábios. Conclusão: indivíduos com DDF apresentaram alteração de simetria facial, ângulo nasolabial e da pele quanto às rugas. Aspectos estéticos da face correlacionaram-se entre si, bem como ângulo nasolabial com sulcos na testa. A idade também demonstrou relação com a estética facial.

RESUMO: Objetivo: verificar a ocorrência de alterações na sensibilidade orofacial e o tempo de recuperação desta após a realização da cirurgia ortognática, em indivíduos com fissura labiopalatina. Métodos: após aprovação do Comitê de Ética em Pesquisa, iniciou-se o estudo o qual analisou os relatórios de exames miofuncionais orofaciais, realizados antes e após a cirurgia ortognática no período de 2012 a 2014. Foram selecionados 53 prontuários de pacientes que realizaram a prova de sensibilidade, com idade entre 18 e 40 anos, de ambos os gêneros (57% masculino e 43% feminino). A prova de sensibilidade foi aplicada nos lábios, na língua, na papila incisiva e mento, a partir do estesiômetro (Semmes-Weintein), antes da cirurgia (2 a 3 dias) e após a cirurgia (3 a 6 meses/9 a 12 meses). Os resultados foram analisados por meio de estatística descritiva para verificar a frequência de alteração da sensibilidade, e as comparações foram realizadas pelos testes de Wilcoxon e Qui-Quadrado (p

Purpose: To evaluate the effect of oral rehabilitation with immediately loaded fixed implant-supported mandibular prostheses on chewing and swallowing in elderly individuals. Materials and Methods: Fifteen completely edentulous patients aged more than 60 years (10 women and five men), wearing removable dentures in both arches, had a mandibular denture replaced by an implant-supported prosthesis. All individuals were evaluated before surgery and again 3, 6, and 18 months later with regard to mastication and swallowing conditions. Examinations entailed an interview, evaluation of tactile sensitivity of the face, and observation of food intake, masticatory type, formations of bolus, and pain during mastication. The swallowing evaluation comprised observation of clinical signs related to the oral and pharyngeal stages of swallowing, as well as the presence of oral residue. The findings of different evaluations before and 3, 6, and 18 months after the surgical-prosthetic procedure were statistically compared by analysis of variance for repeated measurements at a significance level of 5%. Results: The questionnaire revealed a reduction in complaints of masticatory and swallowing disturbances, a decreased need for liquid ingestion, and reduced choking and coughing. Clinical evaluations showed improved oral function and bolus propulsion for both solid and paste-consistency foods; pain during mastication was also resolved. Conclusion: Treatment with mandibular implant-supported dentures had positive effects on the clinical aspects of mastication and swallowing in elderly individuals. [ABSTRACT FROM AUTHOR]

Abstract The pursuit for quality of life urged a better understanding of aspects involved in ageing to minimize its consequences. Although many studies investigated older adults’ voice, aspects affecting this population voice-related quality of life have not yet been explored. Objective To investigate how aerodynamics and vocal aspects are associated with voice-related quality of life in older adults. Methodology fifty-six older adults aged 60 years or above – 39 women and 17 men – were evaluated. The following procedures were performed: application of the Voice-Related Quality of Life (V-RQOL) protocol; vocal assessment, including auditory-perceptual and acoustic analysis, from which we obtained fundamental frequency (F 0 ), standard deviation of fundamental frequency (SDF 0 ), shimmer, amplitude perturbation quotient (APQ), jitter, pitch period perturbation quotient (PPQ), and harmonics to noise ratio (HNR); aerodynamic assessment using a spirometer; and maximum phonation time (MPT) for /a/, /s/, /z/ and number counting. Results older adults tend to present high V-RQOL scores. Among women, roughness, APQ, and HNR parameters were negatively correlated with V-RQOL, whereas F 0 was positively. We found no correlation between spirometry measurements and V-RQOL. MPT for /a/, /z/, and number counting was positively correlated with V-RQOL solely among men. Conclusion Vocal roughness and acoustic parameters have a negative impact on the quality of life of older women. Respiratory aspects related to the available air support for speaking affected the most the voice-related quality of life of older men.

Aim: To establish reference values concerning the results of oral and vocal fold diadochokinesis (DDK) assessment, as well as to analyze the differences among gender and age. Methods: One hundred and fifty Brazilian-Portuguese-speaking children from 8 to 10 years old were assessed. The DDK was assessed through the repetition of 'pa', 'ta', 'ka', 'pataka', 'a' and 'i'. Results: As age increased there was a rise in the average DDK rate, a rise in the coefficient of variation of period for syllable 'ka' and a rise in the coefficient of variation of the peak intensity for the syllable 'ta'. The number of utterances per second of 'ta' was higher for girls than for boys. In trisyllables, 8-year-old girls presented a lower number of utterances than the other subgroups. As age increased, there was a rise in the average DDK rate of the vowel 'i' for girls; there was also a smaller standard deviation and perturbations of the period for this same vowel. Conclusion: Oral and vocal fold DDK reference values were established and differences as to gender and age were observed. Copyright © 2010 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Objective: Histotripsy is a focused ultrasound therapy that ablates tissue via the action of bubble clouds. It is under investigation to treat a number of ailments, including renal tumors. Ultrasound imaging is used to monitor histotripsy, though there remains a lack of definitive imaging metrics to confirm successful treatment outcomes. In this study, a convolutional neural network (CNN) was developed to segment ablation on ultrasound images., Methods: A transfer learning approach was used to replace classification layers of the residual network ResNet-18. Inputs to the classification layers were based on ultrasound images of ablated red blood cell phantoms. Digital photographs served as the ground truth. The efficacy of the CNN was compared to subtraction imaging, and manual segmentation of images by two board-certified radiologists., Results: The CNN had a similar performance to manual segmentation, though was improved relative to segmentation with subtraction imaging. Predictions of the network improved over the course of treatment, with the Dice similarity coefficient less than 20% for fewer than 500 applied pulses, but 85% for more than 750 applied pulses. The network was also applied to ultrasound images of ex vivo kidney exposed to histotripsy, which indicated a morphological shift in the treatment profile relative to the phantoms. These findings were consistent with histology that confirmed ablation of the targeted tissue., Conclusion: Overall, the CNN showed promise as a rapid means to assess outcomes of histotripsy and automate treatment., Significance: Data collected in this study indicate integration of CNN image segmentation to gauge outcomes for histotripsy ablation holds promise for automating treatment procedures.

Objective: The objective of the study was to analyze if individuals with velocardiofacial syndrome (VCFS) present the same characteristics of speech and velopharyngeal function (VPF) compared with patients with nonsyndromic submucous cleft palate, as well as to compare the effectiveness of palate surgery on the speech function and VPF between groups., Methods: This was a prospective study performed at the Speech Therapy Sector and Physiology Laboratory, Hospital for Rehabilitation of Craniofacial Anomalies/University of São Paulo.The procedure performed was primary palatoplasty associated or not to superiorly based pharyngeal flap surgery.There were 50 patients with velopharyngeal insufficiency: 25 with signals of VCFS (VCFS group) and 25 without syndrome with submucous cleft palate (SMCP group).The hypernasality was scored by 3 examiners; nasalance was evaluated by nasometry, and VPF was assessed by the size of the velopharyngeal gap on the nasoendoscopy. The evaluations were conducted before and, in average, 18 months after surgery., Results: Before surgery, the VCFS and SMCP groups presented similar speech function and VPF characteristics in all parameters, with no statistically significant differences. After surgery, there was reduction in the hypernasality, nasalance, and VPF in, respectively, 20%, 31%, and 36% of patients in the VCFS group and in 24%, 30%, and 30% in the SMCP group. Elimination/normalization of variables was obtained in 28%, 19%, and 8% of patients in the VCFS group and 20%, 40%, and 25% in the SMCP group, respectively, for hypernasality, nasalance, and VPF. There was no statistically significant difference between groups., Conclusions: Patients with VCFS presented similar speech function and VPF characteristics as patients with nonsyndromic SMCP. The surgery for velopharyngeal insufficiency correction was equally effective for the improvement and resolution of speech symptoms and VPF in patients with VCFS compared with the SMCP group.

Background: speech production in different modalities of oral prosthetic rehabilitation., Aim: to study the speech production of subjects submitted to different oral rehabilitation modalities., Method: 36 elderly individuals (average = 68 years) of both gender were evaluated. Participants were divided in three groups: 13 subjects with natural teeth (A); 13 edentate using maxillary and mandibular conventional dentures (B); and 10 edentate using maxillary conventional dentures and mandibular implant-supported prosthesis (C). Prosthesis stability was evaluated by a dentist and speech samples were analyzed by five speech-language pathologists. In order to determine the frequency of speech sound alterations, the Percentage of Correct Consonants (PCC) was used., Results: few individuals presented speech alterations. Group C presented a higher occurrence of speech alterations (23.08%). Locked articulation was present in all groups; the reduction in lip movement was observed for groups A and B; exaggerated articulation and absence of saliva control was observed for group C. A smaller Percentage of Correct Consonants value was observed for the linguodental phonemes, in groups B and C, followed by the alveolar phonemes. Group A presented the majority of individuals with no speech alterations. On the other hand, for groups B and C, lips and tongue projection was frequently observed. No statistical difference was found between the groups. Most of the individuals in group B presented unsatisfactory prosthesis stability, but no association was identified between speech alterations and prosthesis stability., Conclusion: despite the small sample, the results of the study suggest that individuals using dentures present alterations in linguodental and alveolar phonemes. The type of prosthesis and its stability do not seem to interfere in speech production.

Temporomandibular dysfunction (TMD) is a complex disturbance that involves the masticatory muscles and/or temporomandibular joint, causing damage to the masticatory function. This study evaluated the electromyographic activity of the masseter muscle during habitual mastication of bread, apple, banana, cashew nut and paraffin film (Parafilm M) in 25 adult subjects, of both gender, with TMD. The results were compared to those of a control group, composed of 15 adult subjects, of both sexes, free of signs and/or symptoms of TMD. The MYO-TRONICS Inc., K6-I computer software was used for electromyographic processing and analyzed the following parameters: duration of the act, duration of the masticatory cycle and number of cycles. No significant differences were found between subjects in the control group and individuals with TMD as to duration of the masticatory act and of the masticatory cycle, considering all materials used for mastication. The duration of the masticatory act and cycle was longer during mastication of paraffin film in both groups. The number of masticatory cycles was higher for mastication of apple in comparison to mastication of banana, in both groups. It can be concluded that the consistency of foods influences the duration parameters of the act, duration of the cycle and the number of masticatory cycles, and the behavior of the masticatory muscles in individuals with TMD during habitual mastication is similar to that verified in individuals without TMD.

The main purpose of this study was to investigate the impact of maxillary advancement on speech resonance in subjects with cleft lip and palate. The study sample was composed of 42 subjects aged 16 to 41 years old with operated cleft palate ± lip submitted to maxillary advancement. Resonance was evaluated before and 3 to 12 months after surgery by perceptual analysis and graduated from absent to severe. It was observed that 47.5% of the subjects presented impairment of resonance after orthognathic surgery, with a confidence interval (at 95%) from 31.5% to 63.9%. These results suggest that orthognathic surgery in individuals with cleft palate may interfere in resonance, causing, or increasing the degree of hypernasality. Therefore, this highlights the importance of the orientation about the risks and benefits of maxillary advancement surgery and follow-up of these patients.