1. Cytostatic and cytotoxic properties of chimeric peptides containing cyclin-inhibiting fragments.
- Author
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Kulinich TM, Kharchenko VP, Filyasova EI, Shishkin AM, and Bozhenko VK
- Subjects
- Cells, Cultured, Cyclin-Dependent Kinase Inhibitor p16 genetics, E2F1 Transcription Factor genetics, Humans, Peptide Fragments genetics, Recombinant Fusion Proteins genetics, Cell Proliferation drug effects, Cyclin-Dependent Kinase Inhibitor p16 metabolism, Cyclins antagonists & inhibitors, Cytostatic Agents metabolism, E2F1 Transcription Factor metabolism, Peptide Fragments metabolism, Recombinant Fusion Proteins metabolism
- Abstract
Properties of chimeric peptides containing cell-penetrating sequences and pl6INK4a and E2F fragments were studied in vitro on immurtal cultures of human cells. Both sequences exhibit cytostatic activity. The peptide containing fragment p16INK4a inhibited proliferation during the G0/G1 stage of the cell cycle, while sequence E2F suppressed proliferation in S phase. Both sequences exhibit cytotoxic properties and can induce apoptosis.
- Published
- 2008
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