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The present investigation was undertaken with the objective to develop high yielding, fine grain rice varieties possessing broad spectrum durable resistance by transferring bacterial leaf blight (BLB) resistant genes viz., xa13 and Xa21 from Improved Pusa Basmati 1. A popular high yielding and fine grain rice variety, HUBR 2-1 (Malviya Dhan 2-1), susceptible to BLB and Improved Pusa Basmati 1 carrying resistant genes for BLB (xa13 & Xa21 genes) were selected as parents for crossing. Improved Pusa Basmati 1 was verified for the presence of target genes by using gene linked primers viz., xa13 promotor and pTA 248. The cross viz., HUBR 2-1 x Improved Pusa Basmati 1 was undertaken during Kharif 2011 and F1 progenies were confirmed during rabi 2011. The F1 plants confirmed as true hybrids for both the genes were advanced to the F2 generation and foreground selection was done using gene linked markers. Genetic analysis in the F2 population confirmed that the genes (xa13 & Xa21) governing BLB resistance followed Mendelian inheritance. The phenotypic data analysis revealed that the plants carrying two resistance gene combinations (xa13xa13 Xa21Xa21, xa13xa13Xa21xa21) showed BLB resistance (0-3 scale), while the gene combinations viz., Xa13Xa13Xa21Xa21, Xa13Xa13Xa21xa21 and Xa13xa13Xa21Xa21, Xa13xa13Xa21xa21 showed BLB resistance (5-30). [ABSTRACT FROM AUTHOR]

Hop is an important source of medicinally valuable secondary metabolites including bioactive prenylated chalcones. To gain in-depth knowledge of the regulatory mechanisms of hop flavonoids biosynthesis, full-length cDNA of Hl Myb8 transcription factor gene was isolated from lupulin glands. The deduced amino acid sequence of Hl Myb8 showed high similarity to a flavonol-specific regulator of phenylpropanoid biosynthesis At MYB12 from Arabidopsis thaliana . Transient expression studies and qRT-PCR analysis of transgenic hop plants overexpressing Hl Myb8 revealed that Hl MYB8 activates expression of chalcone synthase Hl CHS_H1 as well as other structural genes from the flavonoid pathway branch leading to the production of flavonols ( F3H , F’3H , FLS ) but not prenylflavonoids ( PT1 , OMT1 ) or bitter acids ( VPS , PT1 ). Hl Myb8 could cross-activate Arabidopsis flavonol-specific genes but to a much lesser extent than At Myb12. Reciprocally, At Myb12 could cross-activate hop flavonol-specific genes. Transcriptome sequence analysis of hop leaf tissue overexpressing Hl Myb8 confirmed the modulation of several other genes related to flavonoid biosynthesis pathways ( PAL , 4CL , ANR , DFR , LDOX ). Analysis of metabolites in hop female cones confirmed that overexpression of Hl Myb8 does not increase prenylflavonoid or bitter acids content in lupulin glands. It follows from our results that Hl MYB8 plays role in a competition between flavonol and prenylflavonoid or bitter acid pathways by diverting the flux of CHS_H1 gene product and thus, may influence the level of these metabolites in hop lupulin. [ABSTRACT FROM AUTHOR]

In 2018, Corynebacterium sanguinis strain Marseille-P8776 was isolated from the blood of a 64-year-old woman suffering from breast cancer who had undergone chemotherapy and radiotherapy. Following whole-genome sequencing, the chromosome of strain Marseille-P8776 was 2,613,836 bp long with a G+C content of 64.92%, 2,568 protein-coding genes, and 64 RNA genes.