28 results on '"Kingry, Luke C."'
Search Results
2. Metagenomic Detection of Bacterial Zoonotic Pathogens among Febrile Patients, Tanzania, 2007-2009.
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Rolfe, Robert J., Sheldon, Sarah W., Kingry, Luke C., Petersen, Jeannine M., Maro, Venance P., Kinabo, Grace D., Saganda, Wilbrod, Maze, Michael J., Halliday, Jo E. B., Nicholson, William L., Galloway, Renee L., Rubach, Matthew P., and Crump, John A.
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METAGENOMICS ,COXIELLA burnetii ,PATHOGENIC microorganisms ,BARTONELLA ,RICKETTSIA - Abstract
Bacterial zoonoses are established causes of severe febrile illness in East Africa. Within a fever etiology study, we applied a high-throughput 16S rRNA metagenomic assay validated for detecting bacterial zoonotic pathogens. We enrolled febrile patients admitted to 2 referral hospitals in Moshi, Tanzania, during September 2007-April 2009. Among 788 participants, median age was 20 (interquartile range 2-38) years. We performed PCR amplification of V1-V2 variable region 16S rRNA on cell pellet DNA, then metagenomic deep-sequencing and pathogenic taxonomic identification. We detected bacterial zoonotic pathogens in 10 (1.3%) samples: 3 with Rickettsia typhi, 1 R. conorii, 2 Bartonella quintana, 2 pathogenic Leptospira spp., and 1 Coxiella burnetii. One other sample had reads matching a Neoerhlichia spp. previously identified in a patient from South Africa. Our findings indicate that targeted 16S metagenomics can identify bacterial zoonotic pathogens causing severe febrile illness in humans, including potential novel agents. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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3. Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium
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Replogle, Adam J., Sexton, Christopher, Young, John, Kingry, Luke C., Schriefer, Martin E., Dolan, Marc, Johnson, Tammi L., Connally, Neeta P., Padgett, Kerry A., and Petersen, Jeannine M.
- Published
- 2021
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4. Surveillance for and Discovery of Borrelia Species in US Patients Suspected of Tickborne Illness
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Kingry, Luke C., Anacker, Melissa, Pritt, Bobbi, Bjork, Jenna, Respicio-Kingry, Laurel, Liu, Gongping, Sheldon, Sarah, Boxrud, David, Strain, Anna, Oatman, Stephanie, Berry, Jon, Sloan, Lynne, Mead, Paul, Neitzel, David, Kugeler, Kiersten J., and Petersen, Jeannine M.
- Published
- 2018
5. Francisella tularensis Transmission by Solid Organ Transplantation, 2017
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Nelson, Christina A., Murua, Christian, Jones, Jefferson M., Mohler, Kelli, Zhang, Ying, Wiggins, Landon, Kwit, Natalie A., Respicio-Kingry, Laurel, Kingry, Luke C., Petersen, Jeannine M., Brown, Jennifer, Aslam, Saima, Krafft, Melissa, Asad, Shadaba, Dagher, Hikmat N., Ham, John, Medina-Garcia, Luis H., Burns, Kevin, Kelley, Walter E., Hinckley, Alison F., Annambhotla, Pallavi, Carifo, Karen, Gonzalez, Anthony, Helsel, Elizabeth, Iser, Joseph, Johnson, Michael, Fritz, Curtis L., and Basavaraju, Sridhar V.
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United States. Centers for Disease Control and Prevention ,Alcoholism -- Health aspects ,Blood tests -- Health aspects ,Metronidazole -- Health aspects ,Cefepime -- Health aspects ,Genomes -- Health aspects ,Organ transplantation -- Health aspects ,EDTA -- Health aspects ,Genomics -- Health aspects ,Meropenem -- Health aspects ,Surgery ,Tularemia ,Medical equipment ,Pneumonia ,Substance withdrawal syndrome ,Health - Abstract
Tularemia, also known as rabbit fever, is a zoonotic disease caused by the gram-negative bacterium Francisella tularensis. Natural transmission to humans occurs through a variety of routes, including tick and [...]
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- 2019
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6. Whole genome multilocus sequence typing as an epidemiologic tool for Yersinia pestis
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Kingry, Luke C., Rowe, Lori A., Respicio-Kingry, Laurel B., Beard, Charles B., Schriefer, Martin E., and Petersen, Jeannine M.
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- 2016
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7. Immune Response to Mycobacterium tuberculosis and Identification of Molecular Markers of Disease
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Gonzalez-Juarrero, Mercedes, Kingry, Luke C., Ordway, Diane J., Henao-Tamayo, Marcela, Harton, Marisa, Basaraba, Randall J., Hanneman, William H., Orme, Ian M., and Slayden, Richard A.
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- 2009
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8. Borrelia mayonii – A cause of Lyme borreliosis that can be visualized by microscopy of thin blood films
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Pritt, Bobbi S., Fernholz, Emily C., Replogle, Adam J., Kingry, Luke C., Sciotto, Michael P., and Petersen, Jeannine M.
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- 2022
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9. Francisella tularensis Transmission by Solid Organ Transplantation, 20171.
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Nelson, Christina A., Murua, Christian, Jones, Jefferson M., Mohler, Kelli, Ying Zhang, Wiggins, Landon, Kwit, Natalie A., Respicio-Kingry, Laurel, Kingry, Luke C., Petersen, Jeannine M., Brown, Jennifer, Aslam, Saima, Krafft, Melissa, Asad, Shadaba, Dagher, Hikmat N., Ham, John, Medina-Garcia, Luis H., Burns, Kevin, Kelley, Walter E., and Hinckley, Alison F.
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TRANSPLANTATION of organs, tissues, etc. ,FRANCISELLA tularensis ,GRAM-negative bacteria ,HEART transplantation ,HISTORY ,KIDNEY transplantation ,SENTINEL health events ,SURVEYS ,TULAREMIA ,INFECTIOUS disease transmission - Abstract
In July 2017, fever and sepsis developed in 3 recipients of solid organs (1 heart and 2 kidneys) from a common donor in the United States; 1 of the kidney recipients died. Tularemia was suspected only after blood cultures from the surviving kidney recipient grew Francisella species. The organ donor, a middle-aged man from the southwestern United States, had been hospitalized for acute alcohol withdrawal syndrome, pneumonia, and multiorgan failure. F. tularensis subsp. tularensis (clade A2) was cultured from archived spleen tissue from the donor and blood from both kidney recipients. Whole-genome multilocus sequence typing indicated that the isolated strains were indistinguishable. The heart recipient remained seronegative with negative blood cultures but had been receiving antimicrobial drugs for a medical device infection before transplant. Two lagomorph carcasses collected near the donor's residence were positive by PCR for F. tularensis subsp. tularensis (clade A2). This investigation documents F. tularensis transmission by solid organ transplantation. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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10. Whole Genome Sequence and Comparative Genomics of the Novel Lyme Borreliosis Causing Pathogen, Borrelia mayonii.
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Kingry, Luke C., Batra, Dhwani, Replogle, Adam, Rowe, Lori A., Pritt, Bobbi S., and Petersen, Jeannine M.
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NUCLEOTIDE sequencing , *LYME disease , *BORRELIA , *PATHOGENIC bacteria , *COMPARATIVE genomics - Abstract
Borrelia mayonii, a Borrelia burgdorferi sensu lato (Bbsl) genospecies, was recently identified as a cause of Lyme borreliosis (LB) among patients from the upper midwestern United States. By microscopy and PCR, spirochete/genome loads in infected patients were estimated at 105 to 106 per milliliter of blood. Here, we present the full chromosome and plasmid sequences of two B. mayonii isolates, MN14-1420 and MN14-1539, cultured from blood of two of these patients. Whole genome sequencing and assembly was conducted using PacBio long read sequencing (Pacific Biosciences RSII instrument) followed by hierarchical genome-assembly process (HGAP). The B. mayonii genome is ~1.31 Mbp in size (26.9% average GC content) and is comprised of a linear chromosome, 8 linear and 7 circular plasmids. Consistent with its taxonomic designation as a new Bbsl genospecies, the B. mayonii linear chromosome shares only 93.83% average nucleotide identity with other genospecies. Both B. mayonii genomes contain plasmids similar to B. burgdorferi sensu stricto lp54, lp36, lp28-3, lp28-4, lp25, lp17, lp5, 5 cp32s, cp26, and cp9. The vls locus present on lp28-10 of B. mayonii MN14-1420 is remarkably long, being comprised of 24 silent vls cassettes. Genetic differences between the two B. mayonii genomes are limited and include 15 single nucleotide variations as well as 7 fewer silent vls cassettes and a lack of the lp5 plasmid in MN14-1539. Notably, 68 homologs to proteins present in B. burgdorferi sensu stricto appear to be lacking from the B. mayonii genomes. These include the complement inhibitor, CspZ (BB_H06), the fibronectin binding protein, BB_K32, as well as multiple lipoproteins and proteins of unknown function. This study shows the utility of long read sequencing for full genome assembly of Bbsl genomes, identifies putative genome regions of B. mayonii that may be linked to clinical manifestation or tissue tropism, and provides a valuable resource for pathogenicity, diagnostic and vaccine studies. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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11. Comparative review of Francisella tularensis and Francisella novicida.
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Kingry, Luke C. and Petersen, Jeannine M.
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Francisella tularensis is the causative agent of the acute disease tularemia. Due to its extreme infectivity and ability to cause disease upon inhalation, F. tularensis has been classified as a biothreat agent. Two subspecies of F. tularensis, tularensis and holarctica, are responsible for tularemia in humans. In comparison, the closely related species F. novicida very rarely causes human illness and cases that do occur are associated with patients who are immune compromised or have other underlying health problems. Virulence between F. tularensis and F. novicida also differs in laboratory animals. Despite this varying capacity to cause disease, the two species share ~97% nucleotide identity, with F. novicida commonly used as a laboratory surrogate for F. tularensis. As the F. novicida U112 strain is exempt from U.S. select agent regulations, research studies can be carried out in non-registered laboratories lacking specialized containment facilities required for work with virulent F. tularensis strains. This review is designed to highlight phenotypic (clinical, ecological, virulence, and pathogenic) and genomic differences between F. tularensis and F. novicida that warrant maintaining F. novicida and F. tularensis as separate species. Standardized nomenclature for F. novicida is critical for accurate interpretation of experimental results, limiting clinical confusion between F. novicida and F. tularensis and ensuring treatment efficacy studies utilize virulent F. tularensis strains. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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12. The Ly49 gene family. A brief guide to the nomenclature, genetics, and role in intracellular infection.
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Rowe Schenkel, Alan, Kingry, Luke C., and Slayden, Richard A.
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BIOLOGICAL nomenclature ,GENETICS ,PSEUDOGENES ,T cells ,IMMUNOLOGY - Abstract
Understanding the Ly49 gene family can be challenging in terms of nomenclature and genetic organization. The Ly49 gene family has two major gene nomenclature systems, Ly49 and Killer Cell Lectin-like Receptor subfamily A (klra). Mice from different strains have varying numbers of these genes with strain specific allelic variants, duplications, deletions, and pseudogene sequences. Some members activate NK lymphocytes, invariant NKT (iNKT) lymphocytes and γδ T lymphocytes while others inhibit killing activity. One family member, Ly49Q, is expressed only on myeloid cells and is not found on NK, iNKT, or γδ T cells. There is growing evidence that these receptors may regulate not just the immune response to viruses, but other intracellular pathogens as well. Thus, this review's primary goal is to provide a guide for researchers first encountering the Ly49 gene family and a foundation for future studies on the role that these gene products play in the immune response, particularly the response to intracellular viral and bacterial pathogens. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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13. The Francisella tularensis FabI Enoyl-Acyl Carrier Protein Reductase Gene Is Essential to Bacterial Viability and Is Expressed during Infection.
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Kingry, Luke C., Cummings, Jason E., Brookman, Kerry W., Bommineni, Gopal R., Tonge, Peter J., and Slayden, Richard A.
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FRANCISELLA tularensis , *ENOYL-acyl carrier protein reductase (NADH) , *DNA microarrays , *ACYL carrier protein , *ENZYME inhibitors - Abstract
Francisella tularensis is classified as a category A priority pathogen and causes fatal disseminated disease in humans upon inhalation of less than 50 bacteria. Although drugs are available for treatment, they are not ideal because of toxicity and route of delivery, and in some cases patients relapse upon withdrawal. We have an ongoing program to develop novel FAS-II FabI enoyl-ACP reductase enzyme inhibitors for Francisella and other select agents. To establish F. tularensis FabI (FtFabI) as a clinically relevant drug target, we demonstrated that fatty acid biosynthesis and FabI activity are essential for growth even in the presence of exogenous long-chain lipids and that VtfabI is not transcriptionally altered in the presence of exogenous long-chain lipids. Inhibition of FtFabI or fatty acid synthesis results in loss of viability that is not rescued by exogenous long-chain lipid supplementation. Importantly, whole-genome transcriptional profiling of F. tularensis with DNA microarrays from infected tissues revealed that FtfabI and de novo fatty acid biosynthetic genes are transcriptionally active during infection. This is the first demonstration that the FabI enoyl-ACP-reductase enzyme encoded by F. tularensis is essential and not bypassed by exogenous fatty acids and that de novo fatty acid biosynthetic components encoded in F. tularensis are transcriptionally active during infection in the mouse model of tularemia. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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14. Genetic identification of unique immunological responses in mice infected with virulent and attenuated Francisella tularensis
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Kingry, Luke C., Troyer, Ryan M., Marlenee, Nicole L., Bielefeldt-Ohmann, Helle, Bowen, Richard A., Schenkel, Alan R., Dow, Steven W., and Slayden, Richard A.
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IMMUNE response , *FRANCISELLA tularensis , *MICROBIAL virulence , *LABORATORY mice , *PATHOLOGY , *GENE expression , *APOPTOSIS , *ANTIGENS , *DNA microarrays - Abstract
Abstract: Francisella tularensis is a category A select agent based on its infectivity and virulence but disease mechanisms in infection remain poorly understood. Murine pulmonary models of infection were therefore employed to assess and compare dissemination and pathology and to elucidate the host immune response to infection with the highly virulent Type A F. tularensis strain Schu4 versus the less virulent Type B live vaccine strain (LVS). We found that dissemination and pathology in the spleen was significantly greater in mice infected with F. tularensis Schu4 compared to mice infected with F. tularensis LVS. Using gene expression profiling to compare the response to infection with the two F. tularensis strains, we found that there were significant differences in the expression of genes involved in the apoptosis pathway, antigen processing and presentation pathways, and inflammatory response pathways in mice infected with Schu4 when compared to LVS. These transcriptional differences coincided with marked differences in dissemination and severity of organ lesions in mice infected with the Schu4 and LVS strains. Therefore, these findings indicate that altered apoptosis, antigen presentation and production of inflammatory mediators explain the differences in pathogenicity of F. tularensis Schu4 and LVS. [Copyright &y& Elsevier]
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- 2011
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15. Waveguide biosensor with integrated detector array for tuberculosis testing.
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Yan, Rongjin, Lynn, N. Scott, Kingry, Luke C., Yi, Zhangjing, Slayden, Richard A., Dandy, David S., and Lear, Kevin L.
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BIOSENSORS ,WAVEGUIDES ,TUBERCULOSIS ,IMMUNOASSAY ,COMPLEMENTARY metal oxide semiconductors ,PROTEINS ,ANTIGENS ,THICK films - Abstract
A label-free immunoassay using a local evanescent array coupled (LEAC) biosensor is reported. Complementary metal oxide semiconductor chips with integrated photoconductor arrays are used to detect an antibody to a M. tuberculosis protein antigen, HspX. The metrology limits of the LEAC sensor using dc and ac measurement systems correspond to average film thicknesses of 28 and 14 pm, respectively. Limits of detection are 87 and 108 pm, respectively, for mouse immunoglobulin G antibody patterning and antigen detection. [ABSTRACT FROM AUTHOR]
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- 2011
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16. Detection of virus-like nanoparticles via scattering using a chip-scale optical biosensor.
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Yan, Rongjin, Scott Lynn, N., Kingry, Luke C., Yi, Zhangjing, Erickson, Tim, Slayden, Richard A., Dandy, David S., and Lear, Kevin L.
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NANOPARTICLES ,MIE scattering ,POLYSTYRENE ,SCATTERING (Physics) ,BIOSENSORS ,REFRACTIVE index - Abstract
A local evanescent array coupled biosensor is used to detect spherical polystyrene nanoparticles with diameters of 40 nm and 200 nm, whose sizes and refractive index are similar to virus particles. The sensitivity is ∼1%/particle for 200 nm particles and 0.04%/particle for 40 nm particles. Mie scattering in an evanescent field theory is used to model the scattered light intensity for both sizes of nanoparticles. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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17. Francisella tularensis Transmission by Solid Organ Transplantation, 20171.
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Nelson, Christina A., Murua, Christian, Jones, Jefferson M., Mohler, Kelli, Ying Zhang, Wiggins, Landon, Kwit, Natalie A., Respicio-Kingry, Laurel, Kingry, Luke C., Petersen, Jeannine M., Brown, Jennifer, Aslam, Saima, Krafft, Melissa, Asad, Shadaba, Dagher, Hikmat N., Ham, John, Medina-Garcia, Luis H., Burns, Kevin, Kelley, Walter E., and Hinckley, Alison F.
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TRANSPLANTATION of organs, tissues, etc. , *FRANCISELLA tularensis , *GRAM-negative bacteria , *HEART transplantation , *HISTORY , *KIDNEY transplantation , *SENTINEL health events , *SURVEYS , *TULAREMIA , *INFECTIOUS disease transmission - Abstract
In July 2017, fever and sepsis developed in 3 recipients of solid organs (1 heart and 2 kidneys) from a common donor in the United States; 1 of the kidney recipients died. Tularemia was suspected only after blood cultures from the surviving kidney recipient grew Francisella species. The organ donor, a middle-aged man from the southwestern United States, had been hospitalized for acute alcohol withdrawal syndrome, pneumonia, and multiorgan failure. F. tularensis subsp. tularensis (clade A2) was cultured from archived spleen tissue from the donor and blood from both kidney recipients. Whole-genome multilocus sequence typing indicated that the isolated strains were indistinguishable. The heart recipient remained seronegative with negative blood cultures but had been receiving antimicrobial drugs for a medical device infection before transplant. Two lagomorph carcasses collected near the donor's residence were positive by PCR for F. tularensis subsp. tularensis (clade A2). This investigation documents F. tularensis transmission by solid organ transplantation. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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18. Identification of a novel pathogenic Borrelia species causing Lyme borreliosis with unusually high spirochaetaemia: a descriptive study.
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Pritt, Bobbi S, Mead, Paul S, Johnson, Diep K Hoang, Neitzel, David F, Respicio-Kingry, Laurel B, Davis, Jeffrey P, Schiffman, Elizabeth, Sloan, Lynne M, Schriefer, Martin E, Replogle, Adam J, Paskewitz, Susan M, Ray, Julie A, Bjork, Jenna, Steward, Christopher R, Deedon, Alecia, Lee, Xia, Kingry, Luke C, Miller, Tracy K, Feist, Michelle A, and Theel, Elitza S
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LYME disease , *LYME disease treatment , *LYME disease prevention , *SPIROCHAETACEAE , *MICROSCOPY , *PATIENTS , *LYME disease diagnosis , *DNA , *GRAM-negative bacteria , *POLYMERASE chain reaction , *RESEARCH funding , *SPIROCHAETOSIS - Abstract
Background: Lyme borreliosis is the most common tick-borne disease in the northern hemisphere. It is a multisystem disease caused by Borrelia burgdorferi sensu lato genospecies and characterised by tissue localisation and low spirochaetaemia. In this study we aimed to describe a novel Borrelia species causing Lyme borreliosis in the USA.Methods: At the Mayo clinic, from 2003 to 2014, we tested routine clinical diagnostic specimens from patients in the USA with PCR targeting the oppA1 gene of B burgdorferi sensu lato. We identified positive specimens with an atypical PCR result (melting temperature outside of the expected range) by sequencing, microscopy, or culture. We collected Ixodes scapularis ticks from regions of suspected patient tick exposure and tested them by oppA1 PCR.Findings: 100 545 specimens were submitted by physicians for routine PCR from Jan 1, 2003 to Sept 30, 2014. From these samples, six clinical specimens (five blood, one synovial fluid) yielded an atypical oppA1 PCR product, but no atypical results were detected before 2012. Five of the six patients with atypical PCR results had presented with fever, four had diffuse or focal rash, three had symptoms suggestive of neurological inclusion, and two were admitted to hospital. The sixth patient presented with knee pain and swelling. Motile spirochaetes were seen in blood samples from one patient and cultured from blood samples from two patients. Among the five blood specimens, the median oppA1 copy number was 180 times higher than that in 13 specimens that tested positive for B burgdorferi sensu stricto during the same time period. Multigene sequencing identified the spirochaete as a novel B burgdorferi sensu lato genospecies. This same genospecies was detected in ticks collected at a probable patient exposure site.Interpretation: We describe a new pathogenic Borrelia burgdorferi sensu lato genospecies (candidatus Borrelia mayonii) in the upper midwestern USA, which causes Lyme borreliosis with unusually high spirochaetaemia. Clinicians should be aware of this new B burgdorferi sensu lato genospecies, its distinct clinical features, and the usefulness of oppA1 PCR for diagnosis.Funding: US Centers for Disease Control and Prevention Epidemiology and Laboratory Capacity for Infectious Diseases (ELC) Cooperative Agreement and Mayo Clinic Small Grant programme. [ABSTRACT FROM AUTHOR]- Published
- 2016
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19. Detection of Tick-Borne Bacteria from Whole Blood Using 16S Ribosomal RNA Gene PCR Followed by Next-Generation Sequencing.
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Rodino KG, Wolf MJ, Sheldon S, Kingry LC, Petersen JM, Patel R, and Pritt BS
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- Animals, Bacteria genetics, DNA, Bacterial genetics, Genes, rRNA, High-Throughput Nucleotide Sequencing, Polymerase Chain Reaction, RNA, Ribosomal, 16S genetics, Tick-Borne Diseases diagnosis, Ticks
- Abstract
Reported cases of tick-borne diseases have steadily increased for more than a decade. In the United States, a majority of tick-borne infections are caused by bacteria. Clinical diagnosis may be challenging, as tick-borne diseases can present with similar symptoms. Laboratory diagnosis has historically relied on serologic methods, which have limited utility during the acute phase of disease. Pathogen-specific molecular methods have improved early diagnosis, but can be expensive when bundled together and may miss unexpected or novel pathogens. To address these shortcomings, we developed a 16S rRNA gene PCR with a next-generation sequencing (NGS) approach to detect tick-borne bacteria in whole blood. A workflow was optimized by comparing combinations of two extraction platforms and two primer sets, ultimately pursuing DNA extraction from blood with the MagNA Pure 96 and PCR amplification using dual-priming oligonucleotide primers specific to the V1-V3 region of the 16S rRNA gene. The amplified product underwent modified Illumina 16S metagenomics sequencing library preparation and sequencing on a MiSeq V2 Nano flow cell, with data analysis using Pathogenomix RipSeq NGS software. Results with the developed method were compared to those from a V1-V2 16S rRNA gene primer set described by the Centers for Disease Control and Prevention (CDC). The V1-V3 assay demonstrated equivalent performance to the CDC assay, with each method showing concordance with targeted PCR results in 31 of 32 samples, and detecting 22 of 23 expected organisms. These data demonstrate the potential for using a broad-range bacterial detection approach for diagnosis of tick-borne bacterial infection from blood., (Copyright © 2021 American Society for Microbiology.)
- Published
- 2021
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20. Francisella opportunistica sp. nov., isolated from human blood and cerebrospinal fluid.
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Dietrich EA, Kingry LC, Kugeler KJ, Levy C, Yaglom H, Young JW, Mead PS, and Petersen JM
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- Bacterial Typing Techniques, Base Composition, DNA, Bacterial genetics, Francisella isolation & purification, Genes, Bacterial, Humans, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, United States, Blood microbiology, Cerebrospinal Fluid microbiology, Francisella classification, Phylogeny
- Abstract
Two isolates of a Gram-negative, non-spore-forming coccobacillus cultured from the blood and cerebrospinal fluid of immunocompromised patients in the United States were described previously. Biochemical and phylogenetic analyses revealed that they belong to a novel species within the Francisella genus. Here we describe a third isolate of this species, recovered from blood of a febrile patient with renal failure, and formally name the Francisella species. Whole genome comparisons indicated the three isolates display greater than 99.9 % average nucleotide identity (ANI) to each other and are most closely related to the tick endosymbiont F. persica , with only 88.6-88.8 % ANI to the type strain of F. persica . Based on biochemical, metabolic and genomic comparisons, we propose that these three isolates should be recognized as Francisella opportunistica sp. nov, with the type strain of the species, PA05-1188
T , available through the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSM 107100) and the American Type Culture Collection (ATCC BAA-2974).- Published
- 2020
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21. Chromosome and Large Linear Plasmid Sequences of a Borrelia miyamotoi Strain Isolated from Ixodes pacificus Ticks from California.
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Kingry LC, Replogle A, Dolan M, Sexton C, Padgett KA, and Schriefer ME
- Abstract
Borrelia miyamotoi, a relapsing fever group spirochete, is an emerging tick-borne pathogen. It has been identified in ixodid ticks across the Northern Hemisphere, including the West Coast of the United States. We describe the chromosome and large linear plasmid sequence of a B. miyamotoi isolate cultured from a California field-collected Ixodes pacificus tick.
- Published
- 2017
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22. Isolation of the Lyme Disease Spirochete Borrelia mayonii From Naturally Infected Rodents in Minnesota.
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Johnson TL, Graham CB, Hojgaard A, Breuner NE, Maes SE, Boegler KA, Replogle AJ, Kingry LC, Petersen JM, Eisen L, and Eisen RJ
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- Animals, Ixodes microbiology, Ixodes physiology, Lyme Disease microbiology, Minnesota, Borrelia burgdorferi Group isolation & purification, Peromyscus microbiology, Sciuridae microbiology
- Abstract
Borrelia mayonii is a newly described member of the Borrelia burgdorferi sensu lato complex that is vectored by the black-legged tick (Ixodes scapularis Say) and a cause of Lyme disease in Minnesota and Wisconsin. Vertebrate reservoir hosts involved in the enzootic maintenance of B. mayonii have not yet been identified. Here, we describe the first isolation of B. mayonii from naturally infected white-footed mice (Peromyscus leucopus Rafinesque) and an American red squirrel (Tamiasciurus hudsonicus Erxleben) from Minnesota, thus implicating these species as potential reservoir hosts for this newly described spirochete., (Published by Oxford University Press on behalf of Entomological Society of America 2017. This work is written by US Government employees and is in the public domain in the US.)
- Published
- 2017
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23. Toward a Complete North American Borrelia miyamotoi Genome.
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Kingry LC, Replogle A, Batra D, Rowe LA, Sexton C, Dolan M, Connally N, Petersen JM, and Schriefer ME
- Abstract
Borrelia miyamotoi, of the relapsing-fever spirochete group, is an emerging tick-borne pathogen causing human illness in the northern hemisphere. Here, we present the chromosome, eight extrachromosomal linear plasmids, and a draft sequence for five circular and one linear plasmid of a Borrelia miyamotoi strain isolated from an Ixodes sp. tick from Connecticut, USA., (Copyright © 2017 Kingry et al.)
- Published
- 2017
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24. Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States.
- Author
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Pritt BS, Respicio-Kingry LB, Sloan LM, Schriefer ME, Replogle AJ, Bjork J, Liu G, Kingry LC, Mead PS, Neitzel DF, Schiffman E, Hoang Johnson DK, Davis JP, Paskewitz SM, Boxrud D, Deedon A, Lee X, Miller TK, Feist MA, Steward CR, Theel ES, Patel R, Irish CL, and Petersen JM
- Subjects
- Animals, Bacterial Typing Techniques, Borrelia burgdorferi Group genetics, Borrelia burgdorferi Group isolation & purification, DNA, Bacterial genetics, Female, Genes, Bacterial, Humans, Lyme Disease, Midwestern United States, Minnesota, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Wisconsin, Borrelia burgdorferi Group classification, Ixodes microbiology, Phylogeny
- Abstract
Lyme borreliosis (LB) is a multisystem disease caused by spirochetes in the Borrelia burgdorferisensu lato (Bbsl) genospecies complex. We previously described a novel Bbsl genospecies (type strain MN14-1420T) that causes LB among patients with exposures to ticks in the upper midwestern USA. Patients infected with the novel Bbsl genospecies demonstrated higher levels of spirochetemia and somewhat differing clinical symptoms as compared with those infected with other Bbsl genospecies. The organism was detected from human specimens using PCR, microscopy, serology and culture. The taxonomic status was determined using an eight-housekeeping-gene (uvrA, rplB, recG, pyrG, pepX, clpX, clpA and nifS) multi-locus sequence analysis (MLSA) and comparison of 16S rRNA gene, flaB, rrf-rrl, ospC and oppA2 nucleotide sequences. Using a system threshold of 98.3 % similarity for delineation of Bbsl genospecies by MLSA, we demonstrated that the novel species is a member of the Bbsl genospecies complex, most closely related to B. burgdorferisensu stricto (94.7-94.9 % similarity). This same species was identified in Ixodes scapularis ticks collected in Minnesota and Wisconsin. This novel species, Borrelia mayonii sp. nov, is formally described here. The type strain, MN14-1420, is available through the Deutsche Sammlung von Mikroorganismen und Zelkulturen GmbH (DSM 102811) and the American Type Culture Collection (ATCC BAA-2743).
- Published
- 2016
- Full Text
- View/download PDF
25. Chromosome and Linear Plasmid Sequences of a 2015 Human Isolate of the Tick-Borne Relapsing Fever Spirochete, Borrelia turicatae.
- Author
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Kingry LC, Batra D, Replogle A, Sexton C, Rowe L, Stermole BM, Christensen AM, and Schriefer ME
- Abstract
The sequences of the complete linear chromosome and 7 linear plasmids of the relapsing fever spirochete Borrelia turicatae are presented in this report. The 925,547 bp of chromosome and 380,211 bp of plasmid sequence were predicted to contain a total of 1,131 open reading frames, with an average G+C content of 29.7%., (Copyright © 2016 Kingry et al.)
- Published
- 2016
- Full Text
- View/download PDF
26. The Burkholderia pseudomallei enoyl-acyl carrier protein reductase FabI1 is essential for in vivo growth and is the target of a novel chemotherapeutic with efficacy.
- Author
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Cummings JE, Kingry LC, Rholl DA, Schweizer HP, Tonge PJ, and Slayden RA
- Subjects
- Animals, Anti-Bacterial Agents chemistry, Bacterial Proteins antagonists & inhibitors, Bacterial Proteins metabolism, Burkholderia pseudomallei drug effects, Burkholderia pseudomallei enzymology, Burkholderia pseudomallei pathogenicity, Enoyl-(Acyl-Carrier Protein) Reductase (NADPH, B-Specific) antagonists & inhibitors, Enoyl-(Acyl-Carrier Protein) Reductase (NADPH, B-Specific) metabolism, Enzyme Inhibitors chemistry, Female, Gene Knockout Techniques, Isoenzymes antagonists & inhibitors, Isoenzymes genetics, Isoenzymes metabolism, Melioidosis microbiology, Melioidosis mortality, Mice, Mutation, Survival Analysis, Treatment Outcome, Anti-Bacterial Agents pharmacology, Bacterial Proteins genetics, Burkholderia pseudomallei genetics, Enoyl-(Acyl-Carrier Protein) Reductase (NADPH, B-Specific) genetics, Enzyme Inhibitors pharmacology, Melioidosis drug therapy
- Abstract
The bacterial fatty acid biosynthesis pathway is a validated target for the development of novel chemotherapeutics. However, since Burkholderia pseudomallei carries genes that encode both FabI and FabV enoyl-acyl carrier protein (ACP) reductase homologues, the enoyl-ACP reductase that is essential for in vivo growth needs to be defined so that the correct drug target can be chosen for development. Accordingly, ΔfabI1, ΔfabI2, and ΔfabV knockout strains were constructed and tested in a mouse model of infection. Mice infected with a ΔfabI1 strain did not show signs of morbidity, mortality, or dissemination after 30 days of infection compared to the wild-type and ΔfabI2 and ΔfabV mutant strains that had times to mortality of 60 to 84 h. Although signs of morbidity and mortality of ΔfabI2 and ΔfabV strains were not significantly different from those of the wild-type strain, a slight delay was observed. A FabI1-specific inhibitor was used to confirm that inhibition of FabI1 results in reduced bacterial burden and efficacy in an acute B. pseudomallei murine model of infection. This work establishes that FabI1 is required for growth of Burkholderia pseudomallei in vivo and is a potential molecular target for drug development.
- Published
- 2014
- Full Text
- View/download PDF
27. The ly49 gene family. A brief guide to the nomenclature, genetics, and role in intracellular infection.
- Author
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Schenkel AR, Kingry LC, and Slayden RA
- Abstract
Understanding the Ly49 gene family can be challenging in terms of nomenclature and genetic organization. The Ly49 gene family has two major gene nomenclature systems, Ly49 and Killer Cell Lectin-like Receptor subfamily A (klra). Mice from different strains have varying numbers of these genes with strain specific allelic variants, duplications, deletions, and pseudogene sequences. Some members activate NK lymphocytes, invariant NKT (iNKT) lymphocytes and γδ T lymphocytes while others inhibit killing activity. One family member, Ly49Q, is expressed only on myeloid cells and is not found on NK, iNKT, or γδ T cells. There is growing evidence that these receptors may regulate not just the immune response to viruses, but other intracellular pathogens as well. Thus, this review's primary goal is to provide a guide for researchers first encountering the Ly49 gene family and a foundation for future studies on the role that these gene products play in the immune response, particularly the response to intracellular viral and bacterial pathogens.
- Published
- 2013
- Full Text
- View/download PDF
28. An optical waveguide array biosensor for serology - biomed 2010.
- Author
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Yan R, Lynn NS, Kingry LC, Slayden RA, Dandy DS, and Lear KL
- Abstract
A label-free optical waveguide immunosensor was designed, fabricated and tested. Different from other popular resonance-based biosensors, such as surface-plasmon-resonance (SPR) or ring/disk resonance biosensors, the local evanescent array coupled (LEAC) biosensor relies on a local evanescent field shift mechanism and can be readily manufactured using trailing-edge integrated-circuit technology with chip-scale microfluidics technology to provide very low cost. The anticipated final form of the sensor technology will require no external equipment enabling disposable use for point-of-care disease detection in non-traditional health-care settings. The local detection ability enables the LEAC biosensor the capability to detect and identify multiple analytes on the same waveguide. On-chip detection is accomplished by integrating buried polysilicon detector arrays under a silicon nitride waveguide. Observations of antibody-antigen interactions using the LEAC biosensor are reported.
- Published
- 2010
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