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Effect of tea pruning litter biochar (TPLBC) on arsenic (As), cadmium (Cd) and chromium (Cr) content in made tea and successive tea infusions were investigated in a greenhouse experiment with two tea cultivars (TV23 and S.3A/3). Made tea prepared from TV23 and S.3A/3 clone, a decrease in the concentration of As, Cd, and Cr by 36.73%, 16.22%, 13.96%, and 36.63%, 27.78%, 10.54%, respectively over control on the application of the highest dose of TPLBC (500 kg TPLBC ha -1 ). Irrespective of treatments, studied element concentrations were significantly higher (p ≤ 0.05) in the first infusion and lower in the third. Considering Ten g made tea consumption per person per day, the maximum average daily intakes of As, Cd and Cr in a higher dose of TPLBC were far below the tolerable weekly intake prescribed by the World Health Organization. As hazard quotient values of selected elements were ≪ 1, no significant adverse health consequences are expected for tea consumers., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 The Author(s).)