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The family Anaplasmataceae includes tick-borne bacteria of major public and veterinary health interest, as best illustrated by members of the genera Anaplasma and Ehrlichia. Recent epidemiological surveys have also reported on the presence of a novel putative genus in the Anaplasmataceae, Candidatus Allocryptoplasma, previously described as Candidatus Cryptoplasma in the western black-legged tick, Ixodes pacificus. However, the genetic diversity of Ca. Allocryptoplasma and its phylogenetic relationship with other Anaplasmataceae remain unclear. In this study, we developed a multi-locus sequence typing approach, examining the DNA sequence variation at five genes of Ca. Allocryptoplasma found in ticks. Combining this multi-locus sequence typing and genetic data available on public databases, we found that substantial genetic diversity of Ca. Allocryptoplasma is present in Ixodes, Amblyomma and Haemaphysalis spp. ticks on most continents. Further analyses confirmed that the Ca. Allocryptoplasma of ticks, the Ca. Allocryptoplasma of lizards and some Anaplasma-like bacteria of wild mice cluster into a monophyletic genus, divergent from all other genera of the family Anaplasmataceae. Candidatus Allocryptoplasma appears as a sister genus of Anaplasma and, with the genera Ehrlichia and Neoehrlichia, they form a monophyletic subgroup of Anaplasmataceae associated with tick-borne diseases. The detection of genetically distinct Ca. Allocryptoplasma in ticks of significant medical or veterinary interest supports the hypothesis that it is an emergent genus of tick-borne pathogens of general concern.

Introduction: The extensive use of the insecticide chlorpyrifos (CPF) throughout the world has brought increased scrutiny on its environmental and health impact. CPF is a cholinergic neurotoxicant; however, exposure to low noncholinergic doses is associated with numerous neurodevelopmental effects in animal models. In this study, we aimed to assess CPF for its potential to disrupt thyroid hormone signalling and investigate the short- and long-term effects on neurodevelopment by using Xenopus laevis. Methods: The thyroid hormone (TH) disrupting potential of CPF was assessed using TH-sensitive transgenic Tg(thibz:eGFP) tadpoles. The consequences of early embryonic exposure were examined by exposing fertilized eggs for 72 h to environmentally relevant CPF concentrations (10−10 M and 10−8 M). Three endpoints were evaluated: (1) gene expression in whole embryonic brains immediately after exposure, (2) mobility and brain morphology 1 week after exposure, and (3) brain morphology and axon diameters at the end of metamorphosis (2 months after the exposure). Results: CPF disrupted TH signalling in Tg(thibz:eGFP) tadpoles. The expression of genes klf9, cntn4, oatp1c1, and tubb2b was downregulated in response to CPF. Tadpoles exposed to CPF exhibited increased mobility and altered brain morphology compared to control tadpoles. Early embryonic exposure of CPF affected myelinated axon diameter, with exposed animals exhibiting shifted frequency distributions of myelinated axons diameters towards smaller diameters in the hindbrain of froglets. Discussion/Conclusion: This study provides more evidence of the endocrine and neurodevelopment disrupting activity of CPF. Further experimental and epidemiological studies are warranted to determine the long-term consequences of early CPF exposure on brain development. [ABSTRACT FROM AUTHOR]

The expansion of agriculture in equatorial areas is fragmenting and reducing wildlife habitats. For primates, it also increases opportunities to consume crops as high-energy resources, exacerbates conflicts with farmers, and increases exposure to diseases and agrochemicals at the edge of protected areas. In species with sex differences in ranging behavior, individual exposure to such opportunities and threats may vary by sex. Chimpanzees show a great feeding flexibility and are territorial species with varied ranging patterns according to site, sex, or individuals. Within a community whose territory is crossed by a high-traffic road and partially bordered by maize gardens, we tested hypotheses of interindividual differences in access to crops based on age, sex, ranging behavior, and kinship. By analyzing the presence of Sebitoli chimpanzees (Pan troglodytes schweinfurthii , Kibale National Park, Uganda) on video clips from 16 camera traps, we estimated the individual dispersion range across the community's territory and the crop foraging frequency along maize gardens over 16 months. While all age and sex classes were represented at the forest–garden interface, large intrasex differences were observed: some mature males and females were not observed to participate. The crop foraging frequency of adult females in maize gardens was significantly correlated with the location of their ranging areas. Related individuals revealed similar range patterns within the forest territory without sharing crop foraging habits. However, social learning and energy and risks–benefits trade-offs as potential drivers of crop consumption are not excluded. [ABSTRACT FROM AUTHOR]

The IUCN now recognizes the savannah Loxodonta africana and forest Loxodonta cyclotis elephants to be separate species. Despite ecological, behavioural and morphological differences, and different habitat ranges, genetic studies confirm that the two species and hybrids coexist in forest–savannah ecotones. However, the hybrid phenotypes have not yet been described. In this survey we examined whether the phenotypes of the two species and of hybrids can be distinguished. In the first step, we used a machine learning algorithm (K -nearest neighbours) to compare 296 reference images of African elephants from five forest areas and six savannah areas where hybrids have not been recorded, confirming that six morphological criteria can be used to distinguish the species with more than 90% confidence. In the second step, we analysed 1,408 videos of elephants from 14 camera traps in Sebitoli, in Kibale National Park, Uganda, part of the main hybridization area. We used a multiple correspondence analysis and a species assignment key, highlighting the presence of three categories of phenotypes. Compared to the savannah and forest phenotypes (36.8 and 12.1%, respectively), the intermediate phenotypes, which could include hybrids, were more frequent (51.1%). Further studies combining morphology and genetics of the same individuals will be necessary to refine this species assignment key to characterize phenotypes confidently. This non-invasive, fast and inexpensive phenotypical-based method could be a valuable tool for conservation programmes. [ABSTRACT FROM AUTHOR]

Simple Summary: Wild meat is a primary source of protein for many rural populations and its illegal harvest can threaten worldwide wildlife conservation efforts. Near tropical forests, wild meat can be an alternative to domestic meat consumption for different reasons (economy, access, culture, etc.). We aimed to understand drivers of illegal activities affecting wildlife in a hotspot of biodiversity (Sebitoli, Kibale National Park, Uganda) hosting threatened species (including chimpanzees) and bordered by agricultural landscapes with high human densities. Illegal activities were mapped, and individual interviews were conducted. We highlighted that being a young man coming from districts contiguous to Kibale National Park particularly raises the odds of consuming wild meat. This study might help to identify and recommend sustainable social and environmental alternatives to manage biodiversity. The African tropical forests host an inestimable number of resources, including food, medicine, vegetal and animal species. Among them, chimpanzees are threatened with extinction by human activities affecting their habitats, such as forest product harvesting, and/or more directly, snaring and trafficking. We aimed to better understand the spatial distribution of these illegal activities, and the reasons for setting snares and consuming wild meat in an agricultural landscape (subsistence farming and cash crops) densely populated near a protected area (Sebitoli, Northern part of Kibale National Park, Uganda). To carry out this study, we combined GPS records of illegal activities collected with group counts (in total, n = 339 tea workers, 678 villagers, and 1885 children) and individual interviews (n = 74 tea workers, 42 villagers, and 35 children). A quarter of illegal activities collected (n = 1661) targeted animal resources and about 60% were recorded in specific areas (southwest and northeast) of the Sebitoli chimpanzee home range. Wild meat consumption, which is illegal in Uganda, is a relatively common practice among participants (17.1% to 54.1% of respondents depending on actor types and census methods). However, consumers declared that they eat wild meat unfrequently (0.6 to 2.8 times per year). Being a young man coming from districts contiguous to Kibale National Park particularly raises the odds of consuming wild meat. Such an analysis contributes to the understanding of wild meat hunting among traditional rural and agricultural societies from East Africa. [ABSTRACT FROM AUTHOR]

Introduction In a recent study, Hardy et al. (2012) examined ten samples of dental calculus from five Neanderthal individuals from El Sidron in northern Spain (occupation dates between 47 300 [...]

Some animal species have been presumed to be purely diurnal. Yet, they show flexibility in their activity rhythm, and can occasionally be active at night. Recently, it has been suggested that chimpanzees may rarely engage in nocturnal activities in savannah forests, in contrast to the frequent nocturnal feeding of crops observed at Sebitoli, Kibale National Park, Uganda. Here we thus aimed to explore the factors that might trigger such intense nocturnal activity (e.g. harsher weather conditions during daytime, low wild food availability or higher diurnal foraging risk) in this area. We used camera-traps set over 18 km2 operating for 15 months. We report activities and group composition from records obtained either within the forest or at the forest interface with maize fields, the unique crop consumed. Maize is an attractive and accessible food source, although actively guarded by farmers, particularly during daytime. Out of the 19 156 clips collected, 1808 recorded chimpanzees. Of these, night recordings accounted for 3.3% of forest location clips, compared to 41.8% in the maize fields. Most nocturnal clips were obtained after hot days, and most often during maize season for field clips. At night within the forest, chimpanzees were travelling around twilight hours, while when at the border of the fields they were foraging on crops mostly after twilight and in smaller parties. These results suggest that chimpanzees change their activity rhythm to access cultivated resources when human presence and surveillance is lower. This survey provides evidence of behavioral plasticity in chimpanzees in response to neighboring human farming activities, and emphasizes the urgent need to work with local communities to mitigate human-wildlife conflict related to crop-feeding. [ABSTRACT FROM AUTHOR]

The link between dietary antioxidants and oxidative status has been studied extensively in humans. Surprisingly, comparative data are not available from closely related species, such as chimpanzees, which evolved in environments characterized by strong fluctuations in the availability and quality of vegetable food sources. We tested the hypothesis that an abrupt decrease in dietary antioxidants would increase oxidative damages in captive chimpanzees (Pan troglodytes), while a rapid increase in antioxidant intake would decrease oxidative damages accrued while on the low-antioxidant diet. An abrupt decline of dietary antioxidants increased urinary levels of lipid peroxides and of oxidative DNA damage but not of 8-isoprostanes. In contrast, an increased intake of dietary antioxidants did not affect the oxidative status. Chimpanzees that were both older and with a higher dominance rank had lower urinary levels of lipid peroxides and of DNA damage as compared with younger chimpanzees. Neither individual sex nor proportion of time being groomed explained any variation in all three markers of oxidative status. Finally, we found significant within-individual repeatability of all markers of oxidative status over the course of the experiment, suggesting a significant contribution of individual history to molding oxidative status. Our results show that antioxidant intake plays a nonnegligible role in the regulation of oxidative status homeostasis in our closest relatives, the chimpanzees. Our work also suggests that rapid short-term increases in antioxidant intake might not have the desired immediate impact on oxidative status, such as in the case of clinical interventions or training programs. [ABSTRACT FROM AUTHOR]

Abstract Background Malaria parasites (Plasmodium sp.), including new species, have recently been discovered as low grade mixed infections in three wild chimpanzees (Pan troglodytes schweinfurthii) sampled randomly in Kibale National Park, Uganda. This suggested a high prevalence of malaria infection in this community. The clinical course of malaria in chimpanzees and the species of the vectors that transmit their parasites are not known. The fact that these apes display a specific behaviour in which they consume plant parts of low nutritional value but that contain compounds with anti-malarial properties suggests that the apes health might be affected by the parasite. The avoidance of the night-biting anopheline mosquitoes is another potential behavioural adaptation that would lead to a decrease in the number of infectious bites and consequently malaria. Methods Mosquitoes were collected over two years using suction-light traps and yeast-generated CO2 traps at the nesting and the feeding sites of two chimpanzee communities in Kibale National Park. The species of the female Anopheles caught were then determined and the presence of Plasmodium was sought in these insects by PCR amplification. Results The mosquito catches yielded a total of 309 female Anopheles specimens, the only known vectors of malaria parasites of mammalians. These specimens belonged to 10 species, of which Anopheles implexus, Anopheles vinckei and Anopheles demeilloni dominated. Sensitive DNA amplification techniques failed to detect any Plasmodium-positive Anopheles specimens. Humidity and trap height influenced the Anopheles capture success, and there was a negative correlation between nest numbers and mosquito abundance. The anopheline mosquitoes were also less diverse and numerous in sites where chimpanzees were nesting as compared to those where they were feeding. Conclusions These observations suggest that the sites where chimpanzees build their nests every night might be selected, at least in part, in order to minimize contact with anopheline mosquitoes, which might lead to a reduced risk in acquiring malaria infections.

The biological activities of a selected sample of 24 species eaten by wild chimpanzees in the Kibale National Park, western Uganda to assess their potential chemotherapeutic values were measured. It showed that the chimpanzee diet includes compounds that likely offer medicinal benefits, such as reducing the level of pathogens and keeping chimpanzee health problems to a sub clinical level.

Pesticides are used worldwide with potential harmful effects on both fauna and flora. The Kibale National Park in Uganda, a site renowned for its biodiversity is surrounded by tea, banana and eucalyptus plantations as well as maize fields and small farms. We previously showed presence of pesticides with potential endocrine disruptive effects in the vicinity. To further investigate the water pollution linked to agricultural pressure in this protected area, we implemented a complementary monitoring strategy based on: analytical chemistry, effects based methods and the deployment of Polar Organic Chemical Integrative Samplers (POCIS). Chemical analysis of the POCIS extracts revealed the presence of 13 pesticides: carbofuran, DEET, 2.4-D amine, carbaryl, ametryn, isoproturon, metolachlor, terbutryn, dimethoate, imidacloprid, picaridin, thiamethoxam, carbendazim, with the first three being present in the largest quantities. Water samples collected at the POCIS sampling sites exhibited thyroid and estrogen axis disrupting activities in vivo , in addition to developmental and behaviour effects on Xenopus laevis tadpoles model. Based on our observations, for the health of local human and wildlife populations, further monitoring as well as actions to reduce agrochemical use should be considered in the Kibale National Park and in regions exposed to similar conditions. Image 1 • The Kibale national park (KNP) in Uganda is a hotspot of biodiversity. • Wildlife in the northern part of KNP is threatened by the presence of phytochemicals. • Chemical analysis of local river water revealed the presence of 13 pesticides. • Local river water exhibited thyroid and estrogen disrupting activities in vivo. • Further monitoring and pesticide usage reduction should be considered in KNP. Water collected in Kibale national park in Uganda contains 13 pesticides and exhibits thyroid and estrogen axis disruption in aquatic larvae. [ABSTRACT FROM AUTHOR]

Background: Primate populations are in decline, mainly affected by agriculture leading to habitat loss, fragmentation but also chemical pollution. Kibale National Park (Uganda), Sebitoli forest, surrounded by tea and crop fields, is the home range of chimpanzees presenting congenital facial dysplasia. This study aimed to identify to what extent the same phenotypical features are observed in baboons (Papio anubis) of this area. Methods: A total of 25 390 clips recorded by 14 camera traps between January 2017 and April 2018 were analyzed. Results: We identified 30 immature and adult baboons of both sexes with nose and lip deformities. They were more frequently observed in the northwestern part of the area. Conclusions: A possible effect of pesticides used in crops at the border of their habitat is suspected to alter the embryonic development. This study emphasizes the importance of non‐invasive methods to detect health problems in wild primates that can act as sentinels for human health. [ABSTRACT FROM AUTHOR]

International guidelines recommend the integration of local communities within protected areas management as a means to improve conservation efforts. However, local management plans rarely consider communities knowledge about wildlife and their traditions to promote biodiversity conservation. In the Sebitoli area of Kibale National Park, Uganda, the contact of local communities with wildlife has been strictly limited at least since the establishment of the park in 1993. The park has not develop programs, outside of touristic sites, to promote local traditions, knowledge, and beliefs in order to link neighboring community members to nature. To investigate such links, we used a combination of semidirected interviews and participative observations (N= 31) with three communities. While human and wildlife territories are legally disjointed, results show that traditional wildlife and spiritual related knowledge trespasses them and the contact with nature is maintained though practice, culture, and imagination. More than 66% of the people we interviewed have wild animals as totems, and continue to use plants to medicate, cook, or build. Five spirits structure humanwildlife relationships at specific sacred sites. However, this knowledge varies as a function of the location of local communities and the sacred sites. A better integration of local wildlifefriendly knowledge into management plans may revive communities' connectedness to nature, motivate conservation behaviors, and promote biodiversity conservation. [ABSTRACT FROM AUTHOR]

Prenatal exposure to environmental endocrine disruptors can affect development and induce irreversible abnormalities in both humans and wildlife. The northern part of Kibale National Park, a mid-altitude rainforest in western Uganda, is largely surrounded by industrial tea plantations and wildlife using this area (Sebitoli) must cope with proximity to human populations and their activities. The chimpanzees and baboons in this area raid crops (primarily maize) in neighboring gardens. Sixteen young individuals of the 66 chimpanzees monitored (25%) exhibit abnormalities including reduced nostrils, cleft lip, limb deformities, reproductive problems and hypopigmentation. Each pathology could have a congenital component, potentially exacerbated by environmental factors. In addition, at least six of 35 photographed baboons from a Sebitoli troop (17%) have similar severe nasal deformities. Our inquiries in villages and tea factories near Sebitoli revealed use of eight pesticides (glyphosate, cypermethrin, profenofos, mancozeb, metalaxyl, dimethoate, chlorpyrifos and 2,4-D amine). Chemical analysis of samples collected from 2014 to 2016 showed that mean levels of pesticides in fresh maize stems and seeds, soils, and river sediments in the vicinity of the chimpanzee territory exceed recommended limits. Notably, excess levels were found for total DDT and its metabolite pp ′-DDE and for chlorpyrifos in fresh maize seeds and in fish from Sebitoli. Imidacloprid was detected in coated maize seeds planted at the edge the forest and in fish samples from the Sebitoli area, while no pesticides were detected in fish from central park areas. Since some of these pesticides are thyroid hormone disruptors, we postulate that excessive pesticide use in the Sebitoli area may contribute to facial dysplasia in chimpanzees and baboons through this endocrine pathway. Chimpanzees are considered as endangered by IUCN and besides their intrinsic value and status as closely related to humans, they have major economic value in Uganda via ecotourism. Identifying and limiting potential threats to their survival such be a conservation priority. [ABSTRACT FROM AUTHOR]