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Plant growth-promoting rhizobacteria (PGPR) are beneficial bacteria that promote plant growth by adhering to the root surfaces in the rhizosphere region of plants. In addition to improving the physical properties of soils, these bacteria increase plant growth and yield by positively affecting nitrogen fixation, phosphorus solubility, water and nutrient uptake of plants. In this study, the effects of bacteria applications on the vegetative development and yield levels of Karaerik and Narince grape varieties, which are important table varieties of Erzincan and Tokat regions, grown in greenhouses in Erzurum central conditions were investigated. In the study, 4 different bacterial combinations (Pseudomonas chlororaphis + Paenibacillus pabuli + Bacillus simplex + Pseudomonas fluorescens) that promote plant growth were applied to the plant root zone as a solution. In the study, the effects of PGPR applications on the vegetative growth of vines, some pomological characteristics, yield levels, macronutrient contents of leaves and physical and chemical properties of greenhouse soils were determined. While aggregate stability and porosity values of PGPR treated soils increased, water permeability and bulk density values decreased. Bacterial applications in both grape varieties showed a positive effect on shoot length, shoot diameter, number of nodes, berry width, berry length, cluster width, cluster length, number of seeds, number of clusters, cluster weight, number of berries, berry weight, total yield and macronutrient content of leaves. According to the control group, PGPR applied soils; organic matter content increased by 76.2%, aggregate stability values increased by 49.5% and porosity by 5.5%, while water permeability decreased by 18.3% and bulk density by 3.9%. Depending on the application, it was determined that the yield increased by 42.8% in Karaerik grape variety and 35.7% in Narince grape variety. [ABSTRACT FROM AUTHOR]

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In this study, nutraceutical properties of fruit juice and seeds, which are important for human health, of green (Kabarcik, Cavus), red (red Globe) and black (Honusu, Yildiz, Yediveren and Helvani) skin colored grape cultivars grown in same ecological conditions were investigated. Harvest period, number of seeds, cluster form, cluster weight, berry weight, berry color and usage area were determined as morphological parameters. The Folin-Ciocalteu method was used for total phenol content analysis. The total antioxidant status of juices and seeds of grape cultivars have been determined by 2,2–diphenyl−1–picryl-hydrazyl–hydrate (DPPH), ferric reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC) assays. Seeds of all grape cultivars exhibited higher nutraceuticals than fruit juices. Total phenolic content of seed samples was found to be quite variable in range of 1.90 mg GAE/g (cv. Yildiz)–3.46 mg GAE/g (cv. Honusu) fresh weight base (FW), indicating 2 folds of differences between green and black grape cultivars. Green and black cultivars also showed the lowest and the highest total phenolic content in juices between 1.69 (cv. Yediveren) and 2.45 (cv. Honusu mg GAE/g FW). Seeds and fruit juices of all cultivars analyzed showed high antioxidant capacity and total phenol content. Of all different colored cultivars, black peel-colored cultivars had the highest values and combined better morphological and nutraceutical traits with an excellent berry qualitative profile for cv. Honusu and Helvani. [ABSTRACT FROM AUTHOR]

Grapes (Vitis L.), one of the most important and old fruit crops in the world, are grown in a wide range of environments from Australia to North America and from Japan to Chile. All grape growing countries use both international and local cultivars in production to obtain fresh and dried fruits or wine. In Turkey, each region has their own local grape cultivars. Seven local cultivars and one standard grape cultivar, grown in Coruh valley, were analyzed for morphological traits (bunch size, berry color, and berry shape), biochemical characteristics (sugars, organic acids, vitamin C, phenolic compounds, total carotenoids, and flavonoids content), and % inhibition level (antioxidant capacity). The grape cultivars differed from each other in the morphological and biochemical traits. The grape berries contain predominantly nine main phenolic compounds, five organic acids, and two sugars. Among phenolic compounds, chlorogenic acid, syringic acid, rutin, and quercetin were found to be dominant for most of the cultivars between 2.365–5.112 mg/L, 0.923–2.147 mg/L, 0.856–1.711 mg/L, and 0.621–1.347 mg/L, respectively. The local cultivar Kirmizi Istanbul had more chlorogenic acid than the other cultivars. The berries with darker skin color, specifically Kara Turfanda and Nanebur, had higher % inhibition level (antioxidant capacity) than the brighter ones, which correspond also to the results of flavonoid contents. Overall, the local genotypes were found promising due to favorable properties and could be recommended for farmers and consumers. [ABSTRACT FROM AUTHOR]

Grape production has a long history in the Elazig province and surrounding vicinity and produced grapes have been used for table consumption and also processed into traditional beverages, ¸Sıra (special non-alcoholic grape juice) and wine. In the Elazig province, the main grape cultivars are ‘A˘gın Beyazı’, ‘Öküzgözü’, ‘Bo˘gazkere’, ‘¸Silfoni’, ‘Tahannebi’, and ‘Köhnü’. Among them, ‘Köhnü’ cultivar is highly preferred by consumers due to its black color and perfect berry characteristics. The cultivar has grown for centuries in different parts of Elazig and shows a great variability for most of its morphological and biochemical characteristics. In the present study, we aimed to determine morphological and biochemical traits in six ‘Köhnü’ clones sampled from Elazı ˘ g. The cluster weight of six clones was found between 334–394 g. The highest total phenolic content was observed in seeds followed by peel and pulp samples. The seed extract of Clone 2 had the highest total phenolic content at 254 mg gallic acid equivalent/100 g fresh weight. The results also showed that peel, pulp, and seed samples of ‘Köhnü’ grape clones had considerable amounts of antioxidant components determined by DPPH (1,1-Diphenyl-2-picryl-hydrazyl), FRAP (ferric reducing antioxidant power), and TEAC assays and might be rich sources of natural antioxidants. Among the six ‘Köhnü’ clones, Clone 3, and Clone 6 differed from the others in respect to the highest cluster weight, the highest concentrations of total phenolic content, and antioxidant activity. The results also implied that all clones could be used potentially as a readily accessible source of natural antioxidants and as a possible pharmaceutical supplement. [ABSTRACT FROM AUTHOR]

Progress in grape breeding requires the exploitation of genetic variation among market classes, races and gene pools. Wild grapevines (Vitis vinifera ssp. sylvestris) are being endangered in their natural habitats and high priorities should be given to the wild germplasm. Turkey is one of the richest sources of wild grapevine and they mostly grown on forest trees on river basin. The present study was carried out to determine the amount of genetic variation and the degree of relatedness among 23 wild grape genotypes using 17 simple-sequence-repeat markers (SSR). Two international grape cultivars, Cabernet Sauvignon and Merlot are also included study. Number of alleles per locus of the 17 Simple Sequence Repeat (SSR) markers ranged from 3.0 to 14.0 and a total of 162 alleles with an average of 9.53 alleles per locus. The average expected and observed heterozygosity values were 0.773 and 0.781, respectively, which exhibited high level of genetic diversity in the wild grape germplasm. The unweighted pair group method with arithmetic mean analysis revealed three main genetic clusters that partially separated wild grape genotypes each other and. The international cultivars formed a out group. The high genetic diversity among native wild grapes from Coruh valley is suggesting that this area could be one of the centre of diversity of the specie. The results indicate a substantial genetic diversity in V. vinifera ssp. sylvestris and the need of exploring a wider area to increase the chance of finding a particular genotype. [ABSTRACT FROM AUTHOR]

Some ampelographic and biochemical characteristics of eight local grape accessions grown in Coruh valley in Northeastern Turkey were determined. The standard Turkish grape cultivar Cavus was also included the experiment to make better comparison with local ones. Ampelographic characteristics include bunch size, berry color, berry shape and usage. The biochemical parameters were the phenolic compounds, organic acids, vitamin C and specific sugars. The results showed that there was big diversity among local grape accessions in terms of most of the ampelographic and biochemical characteristics. Bunch size ranged from low to medium. Most of the accessions had round berry shape but elliptical, ellipsoidal and oval shape were also determined. The majority of accessions had purple black berry color. Among phenolic compounds compounds chlorogenic acid, syringic acid and rutin were dominant and varied between 1.394 to 4.858 mg/L; 0.731 to 1.934 mg/ L and 0.986 to 1.068 mg/L, respectively. For all accessions, tartaric acid was the dominant (ranged from 2.636 to 5.376 g/L) and followed by malic acid (ranged from 1.079 to 2.646 g/L). Among the sugars, glucose was found to be the major sugar. Overall the accessions evaluated both ampelographic and biochemical characteristics, it is suggested that the majority of accessions had promising characteristics to include them future breeding activities. [ABSTRACT FROM AUTHOR]

It is well known, that grapevine seeds are rich in significant antioxidants. However, the issue of dealing with the analysis and comparison of antioxidant components in the seeds of Vitis vinifera L. in individual cultivars has not yet been sufficiently studied. The experiment was performed with extracts of three varieties (Blaufränkish, Italian Riesling and Cabernet Moravia) and three interspecific cultivars (Nativa, Marlen and Kofranka). Contents of nine major flavonoids (apigenin, astragalin, hyperoside, isorhamnetin, kaempferol, myricetin, quercetin, quercitrin and rutin) and two procyanidins (procyanidin A2 and procyanidin B1) were assessed by the HPLC/MS method. The highest contents of antioxidants were found out in interspecific cultivars Marlen and Nativa while the lowest one was assessed in the cultivar Cabernet Moravia. The most represented flavonoid was hyperoside (cultivar Marlen - 15.66 mg·l-1), least represented was kaempferol (cultivar Cabernet Moravia - 1.81 µg·l-1). [ABSTRACT FROM AUTHOR]

Currant successfully grown in a wide area in Turkey due to its environmental plasticity. The aim of this study is to determine variations in phytochemical contents and antioxidant capacity from certain currant cultivars and genotypes commercially grown in Turkey. Fruit samples taken from two red currant cultivars (‘Red Lake’, ‘Rovada’) and four black (‘S. Nigrum’, ‘Tokat 2’, ‘Tokat 3’ and ‘Tokat 4’) and the genotype 1310 (red currant) were subjected to analysis for phenolic compounds (protocatechuic, vanillic acid, ellagic acid, rutin, quercetin, gallic acid, catechin, chlorogenic acid, caffeic acid, syringic acid, p-coumaric acid, o-coumaric acid, phloridzin and ferulic acid), organic acids (citric acid, tartaric acid, malic acid, succinic acid, and fumaric acid), vitamin C, antioxidant capacity (Trolox equivalent antioxidant capacity [TEAC] assay) and sugars (glucose, fructose and sucrose). Results showed that phytochemical contents and antioxidant capacities statistically varied among currant cultivars and genotype (p<0.05). Caffeic acid was determined only in the genotype 1301. Ellagic acid (1.680 mg/100 g), gallic acid (2.022 mg/100 g), rutin (4.649 mg/100 g), catechin (8.005 mg/100 g) and chlorogenic acid (2.721 mg/100 g) were found the highest values in ‘Tokat 3’, ‘Red Lake’, ‘Tokat 3’, 1310 and ‘S. Nigrum’, respectively. Citric acid, fumaric acid, and malic acid were dominant among organic acids for all cultivars and the genotype 1310. Contents of glucose and fructose among sugars were measured to be higher than content of sucrose for all cultivars and the genotype. The highest antioxidant capacity was detected in cultivar of ‘Rovada’ and the genotype 1310. [ABSTRACT FROM AUTHOR]

Wild edible fruits have been gaining much interest more recently because of their better biochemical content and widely use in ethno medicine treating common disease such as cold, fever and other medicinal claims are now supported with sound scientific evidences. In this study, diversity of some important physicochemical characteristics (plant growth habit, fruit shape, fruit color, fruit weight, pulp ratio, soluble solid content, total phenolics, total anthocyanin, antioxidant activity, sugars, organic acids) of fruits from fourteen promising barberry (Berberis vulgaris L.) selections grown in Erzurum province in Turkey were investigated. Significant differences were observed between the studied genotypes for most of the physicochemical parameters. Fruit weight and pulp ratio were found between 0.102 (25ERZ5) and 0.342 g (25ERZ7) and 60.81% (25ERZ2) and 75.41% (25ERZ11). Total phenolic and anthocyanin content ranged from 2281 (25ERZ5) to 3462 (25ERZ7) as mg GAE per liter fruit juice and 360 to 874 mg as cyanidin-3-glucoside per liter fruit juice. Glucose and fructose were found to be dominant sugars in all barberry accessions analyzed. [ABSTRACT FROM AUTHOR]

Some important physicochemical and bioactive characteristics of disease free 10 wild grown bilberry (Vaccinium myrtillus) accessions were evaluated. External and internal fruit quality was assessed by standard parameters (fruit weight, fruit color, fruit firmness, soluble solids, pH and total acidity) and bioactive contents (total phenolics, total anthocyanins, total antioxidant capacity and, vitamin C) in fruit were also determined. The commercial grown northern higbush blueberry, Vaccinium corymbosum cv. Bluecrop also included in the study to make comparision with bilberry samples. The highbush blueberry cv. Bluecrop had distinctive external fruit characteristics, such as bigger and more attractive fruits. However, the wild grown bilberry accessions showed interesting characters in mesocarp, such as high total phenolic content, total anthocyanin and total antioxidant capacity. Total phenolic and total anthocyanin content was 327 mg gallic acid equivalent and 142 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruit in cv. Bluecrop while it was between 576–624 mg gallic acid equaivalent and 296–324 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruits of bilberry accessions. Moreover, wild accessions approximately had 2 folds higher antioxidant capacity than cv. Bluecrop. Results suggested that bilberry accessions may serve as a source of desirable genes to develop improved varieties that respond to the new needs of the market. [ABSTRACT FROM AUTHOR]

Indigenous grape varieties represent a significant potential for viticultural diversification. Due to fertilization problems, certain varieties from this group require suitable pollenizers for successful fertilization and in order to achieve high-quality grapes. The study was conducted during the years 2016 and 2017 in the vineyard in Herzegovina (southern part of Bosnia and Herzegovina). The aim of this research is to define a suitable pollenizer for the 'Blatina' variety, which has a functionally female flower. Manual pollination was performed with five different pollenizers during the flowering period by applying pollen to the 'Blatina' variety inflorescence during the full bloom stage in the early morning hours. Pollinated inflorescences were isolated, marked, and monitored until the end of the vegetation, while open-pollinated clusters were the control group. The most important characteristics of grape clusters and seeds were analyzed. The best results during the research were obtained by open pollination. The significant effect of the pollenizers was registered in parameters: cluster mass, mass of grape berries in the cluster, number of grape berries per cluster, and average seed mass. Varieties 'Žilavka' and 'Vranac' had better characteristics compared to other pollenizer varieties. The results show that the Blatina variety production with a greater number of pollenizers ensures stable yields. [ABSTRACT FROM AUTHOR]

The Anatolian peninsula has long been linked with the origins of viticulture and winemaking. Erzincan province in Anatolia hosted many civilizations in the past, and each civilization used grapes for different purposes. From past to present, viticulture carried out with the famous 'Karaerik' grape (Vitis vinifera L.) on old traditional Baran training system to avoid cold damage occurred in winter months. During the old civilizations, the cultivar was used only for wine production, but after the first period of the 1900s, this situation changed, and the cultivar was used for table consumption because wine is banned by Islam. The archaeological findings in Erzincan province revealed the cultivar has existed in the province for centuries, and in each historical period, the cultivar was used sustainably, added value to the region, and brought cultural heritage from generation to generation. Grape production in Erzincan province has been a symbol of abundance, fertility and productivity since mythological times. The historical facts indicated that viticulture and winemaking had been a dispensable part of the Erzincan economy and rural development. The vineyards apply the same sustainable management practices from which they receive their grapes. The traditional Baran training system is used for all vineyards. The viticulture in the province has been strongly committed to improving environmental and social sustainability throughout history. [ABSTRACT FROM AUTHOR]

The Erzincan plain is one of the richest regions in Turkey in terms of plant biodiversity. In this region, the famous grape cultivar 'Karaerik' has always dominated grape production due to its berry characteristics. The cultivar shows great morphological variation at clonal level. In this study, the total phenolic content and antioxidant activity of peel, pulp and seed extracts of nine 'Karaerik' clones sampled from same location were investigated. The Folin–Ciocalteu method was used to determine the total phenolic content of peel, pulp and seed extracts of nine clones. To determine antioxidant activity, three well known assays such as DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate), FRAP (Ferric Reducing Antioxidant Power) and TEAC (Trolox Equivalent Antioxidant Capacity) were used. In addition, the correlation between total phenol content and DPPH, FRAP and TEAC was determined. Results showed that among the tissues, seed samples in berries of all clones had the highest total phenol content and antioxidant activity determined by three assays. Seed samples were followed by peel and pulp for total phenolic content and antioxidant activity. Among the nine 'Karaerik' clones, Clone 8 had the highest total phenolic content (149 mg GAE/100 g FW) while Clone 3 had the lowest (111 mg GAE/100 g FW). Peel, pulp and seed samples of nine 'Karaerik' clones showed strong antioxidant activity in DPPH, FRAP and TEAC assays. In particular, grape seeds were found rich for better in phenolic compounds including gallic acid, quercetin, catechin, chlorogenic acid, caffeic acid and p-coumaric acid. Clones such as 7, 8 and 9 higher antioxidant activity may present great potential for grape breeders and the food industry as well as health-conscious consumers. [ABSTRACT FROM AUTHOR]

Erzincan plain is one of the most fascinating regions in Turkey for plant biodiversity. The area is very rich in terms of gene, species and ecosystem diversity. Having a number of natural habitats, mountains, etc., the region is one of the richest regions in Turkey for plant endemism as well. In northern parts of the region, in particular in Üzümlü, Bayırbağ and Pişkidağ districts, grape production dominates agriculture production and the famous 'Karaerik' grape cultivar has been cultivated for a long time on the very special traditional 'Baran' training system to avoid cold damage that occurs in winter months. The cultivar is harvested between 1 September and 1 October according to altitude in the region. The cultivar is well known in Turkey and there is a great demand for this cultivar in Turkey due to its perfect berry characteristics. In this study, yield, marketable product, cluster weight, cluster form, organic acids, specific sugars and sensory characteristics of the 'Karaerik' grape cultivar grown in three altitudes (1200 m a.s.l., 1400 m a.s.l. and 1600 m a.s.l., respectively) in Üzümlü district were investigated. For each altitude, grape clusters were sampled from ten vineyards and an average sample was formed. Marketable product, cluster weight, cluster form, organic acids and specific sugars were determined on those samples. Yield was determined as per decare. Sensory characteristics of samples were determined by five expert panelists. Results showed that the cluster weight was the highest in lower altitude and increasing altitude formed a more conical cluster form compared to winged cylindrical clusters at lower altitudes. The highest yield (740 kg per decare) was obtained in 1200 m a.s.l. and was followed by 1400 m a.s.l. (682 kg per decare) and 1600 m a.s.l. (724 kg per decare), respectively. Altitude strongly affected sugar and organic acid composition and ratio in berries of the 'Karaerik' grape. Fructose and tartaric acid were the main sugar and organic acid at all altitudes and were found between 10.04–14.02 g/100 g and 2.17–3.66 g/100 g, respectively. Sensory scores were also the highest at lower altitudes and decreased parallel to altitude increase. [ABSTRACT FROM AUTHOR]

In this study, berry dimensions and shape traits, which are important for the design of the grape processing system and the classification of 10 different grape varieties grown in same ecological conditions ('Ata Sarısı', 'Barış', 'Dımışkı', 'Hatun Parmağı', 'Helvani', 'Horoz Karası', 'Hönüsü', 'İtalia', 'Mevlana Sarısı', and 'Red Globe') were determined; differences between the varieties were identified with the use of discriminant analysis. The largest grape varieties were identified as 'Ata Sarısı' and 'Red Globe'. The 'Red Globe' and 'Helvani' varieties had geometrically sphere-like shape. The 'Barış' variety had the lowest size averages. According to elliptic Fourier analysis, the primary source of shape variation was ellipse and sphere-looking varieties. However, shape variation was seen due to the existence of a small number of drop-like varieties. According to discriminant analysis, shape differences of the varieties were defined by two discriminant functions. Based on these discriminant functions, the greatest classification performance was achieved for 'Mevlana Sarısı' and 'Dımışkı'. In scatter plots, three shape definitions (sphere, ellipse, and drop) were made for grape varieties. Cluster analysis revealed 4 sub-groups. The first sub-group included the 'Mevlana Sarısı' variety; the second sub-group included the 'Hönüsü', 'Hatun Parmağı', 'Dımışkı', and 'Horoz Karası' varieties; the third sub-group included the 'Ata Sarısı' variety; the fourth sub-group included the 'Barış', 'Helvani', 'İtalia', and 'Red Globe' varieties. The variety in the first group had a geometrically ellipse-like shape, the largest length, and the smallest width. The size data were the smallest for the second sub-group. The third sub-group, with the ellipse-like shape, had the large size data. The grape varieties the closest to the sphere were classified in the fourth group, and these varieties had the large sizes. [ABSTRACT FROM AUTHOR]

Morphology is the most visible and distinct character of plant organs and is accepted as one of the most important tools for plant biologists, plant breeders and growers. A number of methods based on plant morphology are applied to discriminate in particular close cultivars. In this study, image processing analysis was used on 20 grape cultivars ("Amasya beyazı", "Antep karası", "Bahçeli karası", "Çavuş", "Cevşen", "Crimson", "Dimrit", "Erenköy beyazı", "Hafızali", "Karaşabi", "Kırmızı", "İzabella (Isabella) ", "Morşabi", "Müşgüle", "Nuniya", "Royal", "Sultani çekirdeksiz (Sultanina)", "Yalova incisi", "Yerli beyazv", "Yuvarlak çekirdeksiz") to classify them. According to image processing analysis, the longest and the greatest projected area values were observed in "Antep karası" cultivar. The "Sultani çekirdeksiz" cultivar had the least geometric mean diameter. The greatest sphericity ratios were observed in "Yerli beyaz", "Erenköy beyazı" and "Amasya beyazı" cultivars. According to principal component analysis, dimensional attributes were identified as the most significant source of variation discriminant grape cultivars from each other. Morphological differences between the cultivars were explained by sphericity and elongation variables. According to elliptic Fourier analysis (EFA) results, grape morphology largely looks like ellipse and sphere. However, there are some cultivars that look similar to a water drop. The cultivars with similar morphology were identified by a pair-wise comparison test conducted with the use of linear discriminant analysis, and they were presented in a scatter plot. According to cluster analysis, present grape cultivars were classified into seven sub-groups, which indicated great diversity. [ABSTRACT FROM AUTHOR]