1. Immunohistochemical expression of heparanase isoforms and syndecan-1 proteins in colorectal adenomas
- Author
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J. Waisberg, T.R. Theodoro, L.L. Matos, F.B. Orlandi, R.L. Serrano, G.T. Saba, and M.A.S. Pinhal
- Subjects
Syndecan-1 ,colorectal neoplasms ,immunohistochemistry ,heparan sulfate proteoglycans ,glucuronidase ,carcinogenesis. ,Biology (General) ,QH301-705.5 - Abstract
The proteoglycan syndecan-1 and the endoglucuronidases heparanase-1 and heparanase-2 are involved in molecular pathways that deregulate cell adhesion during carcinogenesis. Few studies have examined the expression of syndecan-1, heparanase-1 and mainly heparanase-2 proteins in non-neoplastic and neoplastic human colorectal adenoma tissues. The aim of this study was to analyze the correlation among the heparanase isoforms and the syndecan-1 proteins through immunohistochemical expression in the tissue of colorectal adenomas. Primary anti-human polyclonal anti-HPSE and anti-HPSE2 antibodies and primary anti-human monoclonal anti-SDC1 antibody were used in the immunohistochemical study. The expressions of heparanase-1 and heparanase-2 proteins were determined in tissue samples from 65 colorectal adenomas; the expression of syndecan-1 protein was obtained from 39 (60%) patients. The histological type of adenoma was tubular in 44 (67.7%) patients and tubular-villous in 21 (32.3%); there were no villous adenomas. The polyps were
- Published
- 2016
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