1. From field sampling to pneumatic bioreactor mycelia production of the ectomycorrhizal mushroom Laccaria trichodermophora.
- Author
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Ángeles-Argáiz RE, Carmona-Reyes IA, Quintero-Corrales CA, Medina-Macías FJ, Blancas-Cabrera A, Valdez-Cruz NA, Ulloa M, Trujillo-Roldán MA, and Garibay-Orijel R
- Subjects
- Agaricales, Biomass, Conservation of Natural Resources, Culture Media chemistry, Forests, Oxygen supply & distribution, Seedlings microbiology, Trees microbiology, Bioreactors microbiology, Laccaria growth & development, Laccaria isolation & purification, Mycelium growth & development, Mycorrhizae growth & development, Mycorrhizae isolation & purification
- Abstract
In order to increase survival rates of greenhouse seedlings destined for restoration and conservation programs, successful mycorrhization of the seedlings is necessary. To reforest forest ecosystems, host trees must be inoculated with ectomycorrhizal fungi and, in order to guarantee a sufficient supply of ectomycorrhizal inoculum, it is necessary to develop technologies for the mass production of ectomycorrhizal fungi mycelia. We selected the ectomycorrhizal fungus Laccaria trichodermophora, due to its ecological traits and feasible mycelia production in asymbiotic conditions. Here, we report the field sampling of genetic resources, as well as the highly productive nutritional media and cultivation parameters in solid cultures. Furthermore, in order to achieve high mycelial production, we used strain screening and evaluated pH, carbon source concentration, and culture conditions of submerged cultures in normal and baffled shake flasks. The higher productivity culture conditions in shake flasks were selected for evaluation in a pneumatic bioreactor, using modified BAF media with a 10 g/L glucose, pH 5.5, 25 °C, and a volumetric oxygen transfer coefficient (K
L a) of 36 h-1 . Under those conditions less biomass (12-37 %) was produced in the pneumatic bioreactor compared with the baffled shake flasks. This approach shows that L. trichodermophora can generate a large biomass concentration and constitute the biotechnological foundation of its mycelia mass production., Competing Interests: Declaration of Competing Interest On behalf of all the authors, the corresponding author states that there is no conflict of interest., (Copyright © 2020 British Mycological Society. Published by Elsevier Ltd. All rights reserved.)- Published
- 2020
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