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The study investigates the fabrication and analysis of nickel microparticle-reinforced composites fabricated using the digital light processing (DLP) technique. A slurry is prepared by incorporating Ni-micro particles into a resin vat; it is thoroughly mixed to achieve homogeneity. Turbidity fluctuations are observed, initially peaking at 50% within the first two minutes of mixing and then stabilizing at 30% after 15–60 min. FTIR spectroscopy with varying Ni wt.% is performed to study the alterations in the composite material’s molecular structure and bonding environment. Spectrophotometric analysis revealed distinctive transmittance signatures at specific wavelengths, particularly within the visible light spectrum, with a notable peak at 532 nm. The effects of printing orientation in the X, Y, and Z axes were also studied. Mechanical properties were computed using tensile strength, surface roughness, and hardness. The results indicate substantial enhancements in the tensile properties, with notable increases of 75.5% in the ultimate tensile strength and 160% in the maximum strain. Minimal alterations in surface roughness and hardness suggest favorable printability. Microscopic examination revealed characteristic fracture patterns in the particulate composite at different values for the wt.% of nickel. The findings demonstrate the potential of DLP-fabricated Ni-reinforced composites for applications demanding enhanced mechanical performance while maintaining favorable printability, paving the way for further exploration in this domain.

Summary The design of two overlapping genes in a microbial genome is an emerging technique for adding more reliable control mechanisms in engineered organisms for increased stability. The design of functional overlapping gene pairs is a challenging procedure, and computational design tools are used to improve the efficiency to deploy successful designs in genetically engineered systems. GENTANGLE (Gene Tuples ArraNGed in overLapping Elements) is a high-performance containerized pipeline for the computational design of two overlapping genes translated in different reading frames of the genome. This new software package can be used to design and test gene entanglements for microbial engineering projects using arbitrary sets of user-specified gene pairs. Availability and implementation The GENTANGLE source code and its submodules are freely available on GitHub at https://github.com/BiosecSFA/gentangle. The DATANGLE (DATA for genTANGLE) repository contains related data and results and is freely available on GitHub at https://github.com/BiosecSFA/datangle. The GENTANGLE container is freely available on Singularity Cloud Library at https://cloud.sylabs.io/library/khyox/gentangle/gentangle.sif. The GENTANGLE repository wiki (https://github.com/BiosecSFA/gentangle/wiki), website (https://biosecsfa.github.io/gentangle/), and user manual contain detailed instructions on how to use the different components of software and data, including examples and reproducing the results. The code is licensed under the GNU Affero General Public License version 3 (https://www.gnu.org/licenses/agpl.html). [ABSTRACT FROM AUTHOR]

Research on natural-fiber-reinforced polymer composite is continuously developing. Natural fibers from flora have received considerable attention from researchers because their use in biobased composites is safe and sustainable for the environment. Natural fibers that mixed with Carbon Fiber and or Glass Fiber are low-cost, lightweight, and biodegradable and have lower environmental influences than metal-based materials. This study highlights and comprehensively reviews the natural fibers utilized as reinforcements in polyester composites, including jute, bamboo, sisal, kenaf, flax, and banana. The properties of composite materials consisting of natural and synthetic fibers, such as tensile strength, flexural strength, fatigue, and hardness, are investigated in this study. This paper aims to summarize, classify, and collect studies related to the latest composite hybrid science consisting of natural and synthetic fibers and their applications. Furthermore, this paper includes but is not limited to preparation, mechanism, characterization, and evaluation of hybrid composite laminates in different methods and modes. In general, natural fiber composites produce a larger volume of composite, but their strength is weaker than GFRP/CFRP even with the same number of layers. The use of synthetic fibers combined with natural fibers can provide better strength of hybrid composite.

Background: Sepsis is a dysregulated systemic inflammatory response triggered by infection, resulting in organ dysfunction. A major challenge in clinical pediatrics is to identify sepsis early and then quickly intervene to reduce morbidity and mortality. As blood biomarkers hold promise as early sepsis diagnostic tools, we aimed to measure a large number of blood inflammatory biomarkers from pediatric sepsis patients to determine their predictive ability, as well as their correlations with clinical variables and illness severity scores. Methods: Pediatric patients that met sepsis criteria were enrolled, and clinical data and blood samples were collected. Fifty-eight inflammatory plasma biomarker concentrations were determined using immunoassays. The data were analyzed with both conventional statistics and machine learning. Results: Twenty sepsis patients were enrolled (median age 13 years), with infectious pathogens identified in 75%. Vasopressors were administered to 85% of patients, while 55% received invasive ventilation and 20% were ventilated non-invasively. A total of 24 inflammatory biomarkers were significantly different between sepsis patients and age/sex-matched healthy controls. Nine biomarkers (IL-6, IL-8, MCP-1, M-CSF, IL-1RA, hyaluronan, HSP70, MMP3, and MMP10) yielded AUC parameters > 0.9 (95% CIs: 0.837-1.000; p < 0.001). Boruta feature reduction yielded 6 critical biomarkers with their relative importance: IL-8 (12.2%), MCP-1 (11.6%), HSP70 (11.6%), hyaluronan (11.5%), M-CSF (11.5%), and IL-6 (11.5%); combinations of 2 biomarkers yielded AUC values of 1.00 (95% CI: 1.00–1.00; p < 0.001). Specific biomarkers strongly correlated with illness severity scoring, as well as other clinical variables. IL-3 specifically distinguished bacterial versus viral infection (p < 0.005). Conclusions: Specific inflammatory biomarkers were identified as markers of pediatric sepsis and strongly correlated to both clinical variables and sepsis severity. [ABSTRACT FROM AUTHOR]

Youth (aged 15 to 29 years) account for one quarter of new HIV cases in Canada. Of those, men-who-have-sex-with-men make up one third to one half of new cases in that age range. Moreover, Indigenous youth are over-represented in the proportion of new cases. The use of emtricitabine/tenofovir disoproxil fumarate as pre-exposure prophylaxis (PrEP) significantly reduces the risk of HIV acquisition in adults. Its use was expanded to include youth over 35 kg by the U.S. Food and Drug Administration in 2018. However, PrEP uptake remains low among adolescents. Prescriber-identified barriers include lack of experience, concerns about safety, unfamiliarity with follow-up guidelines, and costs. This article provides an overview of PrEP for youth in Canada, and its associated safety and side effect profiles. Hypothetical case vignettes highlight some of the many demographics of youth who could benefit from PrEP. We present a novel flow diagram that explains the baseline workup, prescribing guidelines, and follow-up recommendations in the Canadian context. Additional counselling points highlight some of the key discussions that should be elicited when prescribing PrEP. [ABSTRACT FROM AUTHOR]

Examines the use of advanced placement programs (AP) in Broward County, Florida, as an intervention strategy to narrow the achievement gap among student groups. Found that AP coursework can be extended to a wider range of students than previously thought, including minority students. (Contains 16 references, 7 tables, and 4 figures.) (Author/MLF)

Social corbiculate bees are major pollinators. They have characteristic bacterial microbiomes associated with their hives and their guts. In honeybees and bumblebees, worker guts contain a microbiome composed of distinctive bacterial taxa shown to benefit hosts. These benefits include stimulating immune and metabolic pathways, digesting or detoxifying food, and defending against pathogens and parasites. Stressors including toxins and poor nutrition disrupt the microbiome and increase susceptibility to opportunistic pathogens. Administering probiotic bacterial strains may improve the health of individual bees and of hives, and several commercial probiotics are available for bees. However, evidence for probiotic benefits is lacking or mixed. Most bacterial species used in commercial probiotics are not native to bee guts. We present new experimental results showing that cultured strains of native bee gut bacteria colonize robustly while bacteria in a commercial probiotic do not establish in bee guts. A defined community of native bee gut bacteria resembles unperturbed native gut communities in its activation of genes for immunity and metabolism in worker bees. Although many questions remain unanswered, the development of natural probiotics for honeybees, or for commercially managed bumblebees, is a promising direction for protecting the health of managed bee colonies. This article is part of the theme issue 'Natural processes influencing pollinator health: from chemistry to landscapes'. [ABSTRACT FROM AUTHOR]

Host-associated microbiomes, particularly gut microbiomes, often harbor related but distinct microbial lineages, but how this diversity arises and is maintained is not well understood. A prerequisite for lineage diversification is reproductive isolation imposed by barriers to gene flow. In host-associated microbes, genetic recombination can be disrupted by confinement to different hosts, for example following host speciation, or by niche partitioning within the same host. Taking advantage of the simple gut microbiome of social bees, we explore the diversification of two groups of gut-associated bacteria, Gilliamella and Snodgrassella, which have evolved for 80 million y with honey bees and bumble bees. Our analyses of sequenced genomes show that these lineages have diversified into discrete populations with limited gene flow. Divergence has occurred between symbionts of different host species and, in some cases, between symbiont lineages within a single host individual. Populations have acquired genes to adapt to specific hosts and ecological niches; for example, Gilliamella lineages differ markedly in abilities to degrade dietary polysaccharides and to use the resulting sugar components. Using engineered fluorescent bacteria in vivo, we show that Gilliamella lineages localize to different hindgut regions, corresponding to differences in their abilities to use spatially concentrated nitrogenous wastes of hosts. Our findings show that bee gut bacteria can diversify due to isolation in different host species and also due to spatial niche partitioning within individual hosts, leading to barriers to gene flow. [ABSTRACT FROM AUTHOR]