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Simple Summary: Defatting can be performed mechanically by cutting the frozen insect larvae and then pressing them to enable the leakage of intracellular fat, or chemically using petroleum ether extraction of the insect meal. This study aimed to investigate the effects of different defatting methods of black soldier fly (Hermetia illucens) larvae meal (BSFM) on the metabolic energy and nutrient digestibility in laying hens. The results show that both defatting methods of BSFM had no adverse effects on the metabolic energy and nutrient digestibility in young laying hens, but pressed black soldier fly meal (BSFMp) demonstrated better effects on the digestibility of metabolic energy and nutrients in the feed for young laying hens. This study aimed to investigate the effects of different defatting methods of black soldier fly (Hermetia illucens) larvae meal (BSFM) on the metabolic energy and nutrient digestibility in laying hens. Sixty young laying hens (Hy-Line W-36) aged 63 days were randomly divided into two groups (G1 and G2), each with five replicates of six hens housed in individual cages. Group G1 was fed 25% pressed black soldier fly meal (BSFMp) and 75% basal diet, and Group G2 was fed 25% extracted black soldier fly meal (BSFMe) and a 75% basal diet. Both diets included 5 g/kg chromium oxide as an external marker. A 7-day preliminary trial was followed by a 4-day experimental period. The results indicate that pressing and extracting significantly affected the digestibility of crude fat and total energy in BSFM, with BSFMp showing significantly higher crude fat digestibility than BSFMe. Similarly, total energy digestibility was also significantly higher in BSFMp. However, there were no significant differences in dry matter, organic matter, and crude protein digestibility between the two processing methods. The apparent metabolic energy values of BSFMp and BSFMe were 16.34 and 12.41 MJ/kg, respectively, showing a significant difference. The nitrogen-corrected metabolic energy values were 15.89 MJ/kg in BSFMp and 11.93 MJ/kg in BSFMe, indicating a highly significant difference. The digestibility of arginine and leucine in BSFMp was significantly higher than in BSFMe, while differences in lysine, cystine, threonine, tryptophan, and isoleucine were not significant. In conclusion, both defatting methods of BSFM had no adverse effects on the metabolic energy and nutrient digestibility in young laying hens, but BSFMp demonstrated better effects on the digestibility of metabolic energy and nutrients in the feed for young laying hens. [ABSTRACT FROM AUTHOR]

The black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), is an insect commonly used for the bioconversion of various organic wastes. Not only can the BSF convert organic waste into macromolecular organic substances, such as insect proteins, but it can also lessen the pollution associated with these waste products by reducing ammonia emissions, for example. In this study, we measured the effects of adding fruit fermentation broth (Fer) and commercial lactic acid bacteria fermentation broth (Em) to kitchen waste (KW), as deodorizing auxiliary substances, on the growth performance of black soldier fly larvae (BSFL), the intestinal flora structure of BSFL, the ammonia emission from the KW substrate, and the microbial community structure of the KW substrate. We found that the addition of Fer or Em increased the body weight of BSFL after 6 d of culture, increasing the growth rate by 9.96% and 7.96%, respectively. The addition of Fer not only reduced the pH of the KW substrate but also increased the relative abundance of probiotics, such as Lactobacillus, Lysinibacillus, and Vagococcus, which inhibited the growth of ammonifiers such as Bacillus, Oligella, Paenalcaligenes, Paenibacillus, Pseudogracilibacillus, and Pseudomonas, resulting in the reduction of ammonia emission in the KW substrate. Moreover, the addition of Fer or Em significantly increased the relative abundances of Bacteroides, Campylobacter, Dysgonomonas, Enterococcus, and Ignatzschineria in the gut of BSFL and increased the species diversity and richness in the KW substrate. Our findings provide a novel way to improve the conversion rate of organic waste and reduce the environmental pollution caused by BSF. [ABSTRACT FROM AUTHOR]

Mastering root distribution is essential for optimizing the root zone environment and for improving water use efficiency, especially for crops cultivated in greenhouses. Here, we set up two irrigation amount levels based on measurements of the cumulative 20 cm pan evaporation (Ep) (i.e., K0.9: 0.9 Ep; K0.5: 0.5 Ep), and three ventilation modes through opening the greenhouse vents at different locations (TR: open the roof vents only; TRS: open both the roof and south vents; TS: open the south vents only) to reveal the effects of the ventilation mode and irrigation amount on the root distribution of greenhouse tomato. Six treatments were designed in blocks with the ventilation mode as the main treatment and the irrigation amount as the vice treatment. On this basis, the normalized root length density (NRLD) model of six treatments was developed by considering air environment, soil water and temperature conditions, root length density (RLD) and yield. The results showed that air speed of the TRS was significantly higher than TR and TS (p < 0.01), and the air temperature and relative humidity under different ventilation showed the rule: TR > TS > TRS. There was a significant third-order polynomial function relationship between NRLD and soil depth, and the coefficient of the cubic term (R0) had a bivariate quadratic polynomial function relationship with irrigation amount and air speed (determination coefficient, R2 = 0.86). Root mean square errors of the simulated and measured value of NRLD under TR, TRS and TS were 0.20, 0.23 and 0.27 in 2020, and 0.31, 0.23 and 0.28 in 2021, respectively, normalized root mean squared errors were 15%, 17%, 20% in 2020, and 23%, 18% and 21% in 2021. The RLD distribution ratio from the ground surface to a one-quarter relative root depth was 74.1%, and 88.0% from the surface to a one-half relative root depth. The results of the yield showed that a better combination of ventilation and irrigation was recommended as TRS combined with K0.9. [ABSTRACT FROM AUTHOR]

Background and Objectives: After failed epilepsy surgery, patients often revert to an antiseizure medication (ASM) ASM regimen, which can be adjusted or optimized in three ways: increasing the dose, alternative therapy, and combination therapy. It is unclear which type of antiseizure medication adjustment method can improve outcomes. Materials and Methods: Children who underwent failed epileptic resection surgery at the Department of Neurosurgery, Children's Hospital of Chongqing Medical University between January 2015 and December 2021 were included in this cohort, who were reviewed for whether they underwent adjustment of ASM with increased dose, alternative therapy, or combination therapy. The seizure outcome and quality of life (QoL) were assessed. Two-tailed Fisher exact test and Mann–Whitney U test were used for statistical analysis. Results: Sixty-three children with failed surgery were included for further analysis, with a median follow-up time of 53 months. The median seizure recurrence time was 4 months. At the last follow-up, 36.5% (n = 23) of patients achieved seizure freedom, 41.3% (n = 26) achieved seizure remission, and 61.9% (n = 39) had a good QoL. None of the three types of ASM adjustment improved children's outcomes, whether considered in terms of seizure-free rate, seizure remission rate, or QoL. Early recurrences were significantly associated with decreased probability of seizure freedom (p = 0.02), seizure remission (p = 0.02), and a good QoL (p = 0.01). Conclusions: Children who underwent failed epilepsy surgery remains some potential for late seizure remission from ASM. Yet adjusting ASM regimen does not increase the probability of seizure remission nor does it improve the QoL. Clinicians should complete evaluations and consider the need for other antiepileptic treatment as soon as possible after surgery failed, especially when dealing with children with an early recurrence. [ABSTRACT FROM AUTHOR]

Drought is complex and is also one of the main disasters affecting China. Exploring the response of agricultural drought and meteorological drought to climatic factors helps us to understand the causes of drought. In this paper, we evaluated the temporal and spatial characteristics of soil drought and meteorological drought (SMD) and explored their responses to climatic factors and latent heat fluxes (LHF), and then explained their variation from the perspective of atmospheric circulation. The following results were obtained. (1) Meteorological drought has gradually increased in the Liaohe River Basin, on the North China Plain, and on the Loess Plateau while average soil moisture has been maintained at only approximately 25%. The impacts of drought are very serious in these regions. (2) LHF response to short time-scale (3-month scale) drought performance is high in the dry season, and the regions with high correlation coefficients are spatially distributed and concentrated in the monsoon climate zone. The regions with high correlation coefficients between drought and LHFs on long time scales (12-month scale) are concentrated in the coastal basin of southeast China. (3) Short- and long-term SMDs showed highly responsive and significant relationships with PDO, showing variations in the southeast coastal basin, the Pearl River basin, the northwest inland basin and the eastern part of the Heilongjiang basin, with a maximum correlation coefficient of 0.21 (p < 0.01). The short-term SMD in the northwestern inland region was significantly negatively correlated with AMO (correlation coefficient of −0.19, p < 0.01). the Nino3.4 index is significantly positively correlated with the SMD in the southeast coastal region of China, with a maximum correlation coefficient of 0.23 (p < 0.01). The decrease in convective precipitation led to a stronger association between soil and meteorological drought and climatic factors. This study helps to reveal the changing patterns of SMDs and can also be used globally to identify the local development patterns of drought under climate change. [ABSTRACT FROM AUTHOR]

Simple Summary: Rosmarinic acid (RA) is a natural water-soluble phenolic compound and a major antioxidant occurring in rosemary. In this study, the effects of dietary supplementation with RA on the growth performance, serum biochemical indices, liver antioxidant activity, and muscle nutritional components of broiler chickens were examined. The results showed that supplementation with RA significantly improved the growth performance of broilers. Therefore, the addition of RA to broiler feed as a natural antioxidant has great prospects. The aim of the present study was to investigate the effects of dietary supplementation with different doses of rosmarinic acid (RA) on the growth performance, serum biochemical indices, liver antioxidant activity, and muscle nutritional component of broiler chickens during 1–20 days of age. A total of 1000 1-day-old Cobb 500 white-feathered broilers were divided into five groups. Each group included four replicates and 50 birds per replicate. The control group was the basic fodder group fed with a basal diet. The experimental groups included four levels of RA (100, 200, 300, and 400 mg/kg RA added to the basal diet, respectively). The results showed that a quadratic increase in final body weight (BW) and average daily gain (ADG) and a quadratic decrease in the feed/gain (F/G) ratio were observed with increasing dietary RA levels. At 200 mg/kg RA supplementation, growth performance was significantly improved. Moreover, under this RA treatment, the highest levels of total protein and globulin were detected in the serum, the activities of total superoxide dismutase and catalase in the liver of broilers were significantly increased, and the malondialdehyde content was significantly decreased, indicating a higher antioxidant capacity of the liver when 200 mg/kg RA was added to the basal diet. The flavor of the muscle meat was improved by dietary supplementation with 200 mg/kg RA. Therefore, adding 200 mg/kg of RA to the diet could significantly improve the growth performance of broilers, improve liver antioxidant capacity, and improve muscle quality, etc. The addition of RA to broiler feed as a natural antioxidant has great prospects. [ABSTRACT FROM AUTHOR]

Drought is a multifaceted natural disaster that can impact the ecological environment, crop yield, and social economy through the hydrological cycle process. Meteorological drought occurs first, which then propagates to other forms. This study presents the propagation characteristics of meteorological to hydrological drought in different river basins of China. The main drivers of drought propagation are also quantitatively analyzed in this study. The standardized precipitation index (SPI) and standardized runoff index (SRI) were used to describe meteorological and hydrological drought, respectively. The Songhua and Liaohe River Basin (SLRB), Haihe River Basin (HARB), Huaihe River Basin (HURB), Yellow River Basin (YRB), Yangtze River Basin (YARB), Pearl River Basin (PRB), Southeast Basin (SEB), Southwest Basin (SWB), and Continental Basin (CB) were analyzed in this study. The precipitation and runoff datasets were used to compute the SPI and SRI, respectively. The results showed that the drought propagation time was mainly 1–3 months in China. In general, drought propagation had a stronger relationship in the central and eastern river basins of China than in the western river basins (SWB and CB). Spring and winter had a weaker drought propagation relationship than autumn and winter. Drought propagation was driven by precipitation in the HURB, YARB, SEB, and PRB; soil moisture and precipitation were drivers in the HARB and YRB; moreover, soil moisture and potential evapotranspiration were drivers in the SLRB and CB. This study improves the understanding of the characteristics and drivers of drought propagation in droughts in river basins. Therefore, this study might provide a reference to reveal the mechanism of drought. [ABSTRACT FROM AUTHOR]

As an important part of the large-scale ecological restoration project of "Grain for Green", the planting area of jujube (Ziziphus jujuba) trees has increased significantly in the hilly region on the Loess Plateau of China, which aims to improve water and soil conservation and develop economic prospects of the region. Understanding the long-term effects of expanding orchards and the responses of soil water dynamics to drought are important for orchard management. Therefore, we use a space-for-time substitution to investigate the variations of plant-available water storage in returning cropland to orchards with different stand ages (2, 6, 10, and 15 years) in a normal year (NY2014, 442.1 mm rainfall) and the next year with low annual precipitation (DY2015, 388 mm rainfall). The results showed that the plant-available water storage in jujube orchards decreased with increasing stand age, and the trend was most obvious in the 60–180 cm layer. The mature stands (10 and 15 years) primarily absorbed soil water from the deep layer (180–300 cm) in DY2015, leading to negative values of plant-available water storage. The whole soil profiles were all subjected to severe water deficits in our study. The findings will help guide rain-fed orchard management in the loess hilly region of China and similar dryland regions. [ABSTRACT FROM AUTHOR]

The black soldier fly (Hermetia illucens) larvae meal (BSFM) has been widely applied in aquaculture production due to its rich nutritional value, easy availability, and renewability. However, to our knowledge, it has not been used in the diets for turtles. Here, we evaluated the acceptability of partial substitution of fish meal (FM) with defatted BSFM in the diets of juvenile Chinese soft-shelled turtles (Pelodiscus sinensis). A total of 225 juvenile turtles were randomly divided into D1, D2, D3, D4, and D5 groups, which were fed with BSFM replacing 0, 5%, 10%, 15%, and 20% FM, respectively, for 70 days. The results showed that growth performances of groups D2 and D3 were not significantly different (P > 0.05) from that of group D1, whereas the growth performance compromised (P < 0.05) in groups D4 and D5. The activity of serum alkaline phosphatase was higher (P < 0.05) in group D3 relative to other groups, while alanine aminotransferase and aspartate aminotransferase activities were higher (P < 0.05) in group D4 when compared with groups D1, D2, and D3. Liver lysozyme activity and malonaldehyde level were significantly higher (P < 0.05) in group D1 relative to other groups, whereas total antioxidant capacity activity showed the opposite trend (P < 0.05). The activities of hepatic total superoxide dismutase and glutathione peroxidase displayed a linear elevation (P = 0.011 and P < 0.001 , respectively) from groups D1 to D3. Intestinal amylase and protease activities linearly increased (P < 0.001) with increasing BSFM levels, while intestinal lipase activity showed a quadratic increase and then a decrease with increasing BSFM levels (P = 0.011). Muscle crude protein and fat contents increased (P < 0.05) in group D3 when compared with groups D1 and D2. Muscle phenylalanine, glutamic, tryptophan, and arginine levels increased in group D1 in comparison with the other groups (P < 0.05), whereas the opposite was true for isoleucine and proline levels. The broken-line analysis based on specific growth rate estimated that the optimal level for replacing dietary FM with BSFM is 5.0%, which could elicit benefits on both the growth performance and physiological health condition of the juvenile Chinese soft-shelled turtles. [ABSTRACT FROM AUTHOR]

A lipidomics approach based on liquid chromatography–tandem mass spectrometry (LC-MS) was applied to analyze the molecular-level mechanism of lipid deposition in Hermetia illucens (H. illucens) larvae fed food waste (FW) at different days of age. The H. illucens larvae reared on FW substrates generally became larger, heavier, and fatter at 5–15 d of age. A large amount of glycerolipids (GL) were deposited, while glycerophospholipids (GP), sphingolipids, and derivatized lipids became relatively less abundant during the growth stage of the larvae. Forty-three subclasses of 3,205 lipid molecules were identified in larvae, and 139 lipids (79 upregulated and 60 downregulated during larval growth and development) were identified as potential biomarkers (variable importance in projection > 1; P < 0.05). The differential lipids were mainly enriched in 19 metabolic pathways, of which 9 metabolic pathways related to lipids, including GL and GP metabolisms. The results demonstrate that the lipid composition and mechanisms changed during the growth and development stage of H. illucens larvae. To the best of our knowledge, this is the first work exploring the molecular-level mechanism of lipid deposition during the growth and development stage of H. illucens larvae. The findings provide novel information for determining and utilizing the nutritional value of H. illucens larvae. [ABSTRACT FROM AUTHOR]

Background: We investigated the proportion of pediatric patients with cerebral paragonimiasis and intracranial hemorrhage who have intracranial pseudoaneurysms.Methods: Images of 17 pediatric patients with cerebral paragonimiasis that first manifested as secondary intracranial hemorrhage were evaluated. All patients underwent computed tomographic angiography before surgery. A diagnosis of cerebral paragonimiasis was confirmed based on a positive Paragonimus-specific antibody test in serum samples from all 17 patients. Cerebral paragonimiasis in five of the 17 patients was further confirmed by histopathological examination of surgical specimens.Results: Computed tomographic angiographic images for six of the 17 patients (35.3%) showed the presence of intracranial pseudoaneurysms. Follow-up computed tomographic angiographic scans two years later showed that two of the six patients had persistent pseudoaneurysms and underwent aneurysmectomy. The diagnosis of pseudoaneurysm was confirmed by histopathological examination postsurgery. In another two of the six patients, the pseudoaneurysm lesions were absorbed and could no longer be seen on three- to six-month follow-up scans. The final two patients with pseudoaneurysms are still under follow-up. Intracranial pseudoaneurysms with various degrees of surrounding hemorrhage were frequently observed at first manifestation.Conclusions: The rupture of intracranial pseudoaneurysms is a common characteristic feature of secondary intracranial hemorrhage caused by cerebral paragonimiasis in pediatric patients. [ABSTRACT FROM AUTHOR]

Glioblastomas are brain tumors with extensive vascularization that are associated with tumor malignancy. The phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway is activated in endothelial cell tumors, although its exact function in glioblastoma neovascularization is poorly characterized. The present study identified that endothelial cells derived from human glioblastomas exhibit increased permeability and motility compared with normal brain vascular endothelial cells. Furthermore, the phosphorylation of AKT was significantly induced in glioblastoma-derived endothelial cells and glioblastoma vessels. To the best of our knowledge, the present study demonstrated for the first time that the cell-cell adhesion junction protein Afadin is phosphorylated and re-localized in glioblastoma-derived endothelial cells, and the phosphorylation and re-localization of Afadin is PI3K/AKT pathway-dependent. AKT-mediated phosphorylation and re-localization of Afadin may be critically involved in the modulation of brain endothelial permeability and migration. Therapies targeting the PI3K/AKT/Afadin pathway may therefore be beneficial for reducing the angiogenic potential of glioblastoma. [ABSTRACT FROM AUTHOR]

Planting economic trees is one of the primary parts of the large-scale ecological rehabilitation project (known as 'Grain for Green' project) initiated in 1999 on the Loess Plateau of China. Knowledge of soil water dynamics and fine roots patterns is critical to water management for orchards in this region. Here we investigated the distribution of root-zone soil water variation and fine roots with a range of stand ages (2, 6, 10 and 15 years) in a rain-fed jujube ( Ziziphus jujuba) plantation on the Loess Plateau. Soil water in the 0- to 3-m depth was monitored using portable Time Domain Reflectometry, and root samples were taken with a hand auger ( Φ = 9 cm). The results showed soil water storage in the 0- to 0·6-m soil layers in all stands increased remarkably following apparent rainfall events. However, there was no clear change in soil water storage below 0·6 m throughout the growing season. Generally, fine root length density increased with the increase of stand age, which were unevenly distributed in the vertical and horizontal directions around the tree trunks in young stands (2 and 6 years), and the fine roots were uniformly distributed radically from the tree trunk in mature stands (10 and 15 years). Additionally, a high-density zone of fine roots was observed in the middle of the inter-row in mature stands, and the specific length of fine roots in young stands was higher than observed in mature stands. The results presented here would be helpful to water management in the rain-fed jujube plantations on Loess Plateau and potentially for other semi-arid orchards. Copyright © 2016 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Spinal cord injury (SCI) is a common disease worldwide that causes permanent neuronal dysfunction without an effective treatment. Long propriospinal neurons (LPSNs) that are spared from injury play a key role in spontaneous recovery after SCI. Traumatic injury of the central nervous system can activate autophagy, which could be a target in the development of a new therapeutic strategy to prevent neuronal loss. Our research focused on whether autophagy is involved in the loss of LPSNs after introducing spinal cord injury in adult rats. Different sacrifice time points were chosen to characterize autophagy and apoptosis. Autophagy and a blocked autophagy flux reached their peaks at 3 d after injury, while apoptosis reached its peak at 7 d after injury when the number of LPSNs significantly decreased. Both autophagy and apoptosis contributed to the loss of LPSNs, and apoptosis was the main cause of cell death. However, autophagy may prevent programmed LPSN cell death (apoptosis), which could promote cell survival. [ABSTRACT FROM AUTHOR]

Objective: The optimal management for middle fossa arachnoid cysts (MFAC) remains controversial, but cystocistern fenestration is effective. MFACs are divided into non-communicating arachnoid cysts (NCIAC) and communicating arachnoid cysts (CIAC). This retrospective observational study evaluated the role of phase-contrast cine magnetic resonance imaging (PC-MRI) in the diagnosis and postoperative evaluation of MFACs and assessed surgical outcomes. Methods: Twenty-eight children with MFAC that were diagnosed by conventional neuroradiology were enrolled. PC-MRI was performed preoperatively. The clinical manifestations, cyst volumes and PC-MRI data were analyzed, and the flow of cerebrospinal fluid (CSF) at the fistula was qualitatively evaluated. Results: No communication with the adjacent subarachnoid space was found on preoperative PC-MRI in 20 patients, and they were diagnosed with NCIAC. A statistically significant decrease in cyst volume was observed in all 20 patients postoperatively. Symptoms resolved or improved in the 14 patients who were symptomatic preoperatively, and a positive CSF flow was found in all 20 patients postoperatively. PC-MRI diagnosed eight patients with CIAC, and the cyst sizes did not increase during follow-up. Conclusions: Cystocistern fenestration is an effective treatment for children with lateral fissure IAC. PC-MRI can be used to determine the IAC type and to evaluate the effectiveness of cystocistern fenestration. [ABSTRACT FROM AUTHOR]

Diffusion tensor imaging (DTI) parameters can detect changes in the brain microstructure in mild traumatic brain injury (mTBI). Whether these parameter changes can predict neural functional recovery after mTBI is still relatively unknown. The present study aimed to investigate the radiological-prognostic correlation between these radiological parameters and learning and memory deficits using an in-house constructed rat model of mTBI. We established a rat model of diffuse axonal injury (DAI) at different injury levels, followed by magnetic resonance imaging at 6, 24, and 72 h, and 1, 2 weeks post injury, and randomly selected the rats for analysis of histopathology and learning and memory deficits. DTI parameters and β-amyloid precursor protein (β-APP) levels were obtained to estimate the extent of brain injury and the correlation with the times of crossing the safety platform as measured using a water maze test. The results revealed that fractional anisotropy (FA) was sensitive to axonal integrity. FA values of the corpus callosum in the injury groups decreased at all time points post injury, except in the mild injury group, which recovered to normal levels at 1 and 2 weeks post-injury. The neural function of the mild injury group recovered to normal compared with the normal control group. FA value, β-APP of corpus callosum in different groups at 24 h post injury showed obvious correlation with learning and memory deficits at the recovery stage (r=0.881, r=-0.931). In conclusion, DTI can reflect varying injury states of DAI over time with direct comparison to histopathology and could be used to predict the neural functional recovery at the early stage post-injury in a rat model. [ABSTRACT FROM AUTHOR]

Soil water is the most critical factor influencing the growth and productivity of crops on the Loess Plateau of China. The popular clean-cultivation soil management practice in rainfed jujube orchards in this region causes a variety of environmental problems, including serious soil erosion and soil quality degradation. Agroforestry is a promising avenue to cope with this problem but its effect on soil water in the root zone is yet to be elucidated. In this study, two different agroforestry systems were established in jujube orchards on the hillslopes of the Loess Plateau, to test the effects on root-zone soil water. The Hemerocallis fulva and fodder Brassica napus were planted between jujube rows. The results showed that both agroforestry systems clearly improved soil water at depths of 0–20 cm and 20–60 cm under jujube trees, however, they apparently reduced the inter-row soil water at depths of 60–120 cm and 120–180 cm in different years compared to the control. A simple method, based on the difference of soil water content between inter-row crops and jujube trees (SWDR), was introduced to judge the possibility of water competition happening. The analyses based on this method showed that in the jujube- H. fulva intercropping system, H. fulva frequently competed soil water with jujube trees in the 0–120 cm depth primarily in the relatively dry period (May to July). However, there was almost no water competition occurred in the jujube- B . napus treatment. Overall, fodder B. napus may be more appropriate than Hemerocallis fulva for intercropping with jujube trees. The results here can provide insights into ground management practice of sloping orchards in water-limited areas. [ABSTRACT FROM AUTHOR]

Spinal cord injury (SCI) often causes a disturbance in the microenvironment in the lesion site resulting in sudden loss of sensory and motor function. Transplantation of stem cells provides a promising strategy in the treatment of SCI. But limited growth and immunological incompatibility of the stem cells with the host limits the application of this strategy. In order to get better survival and integration with the host, we employed a hyaluronic acid (HA) based scaffold covalently modified by poly- l -Lysine (PLL) as a vehicle to deliver the human bone marrow stromal cells (BMSCs) to the injured spinal cord of rats. The BMSCs were chosen as an ideal candidate for its advantage of low expression of major histocompatibility complex II. The data unexpectedly showed that the hetero-transplanted cells survived well in the lesion site even at 8 weeks post injury. Both the immunofluorescent and the electrophysiological assay indicated better survival of the transplanted cells and improved axonal growth in SCI rats transplanted with BMSCs in HA-PLL in contrast to the groups without either BMSCs or the HA scaffold transplantation. These promotions may account for the functional recovery assessed by Basso–Beattie–Bresnahan (BBB) locomotor rating scale in the HA-PLL seeded with BMSCs group. These data suggests that hetero-transplantation of human BMSCs delivered by HA scaffold demonstrates a significant role in the functional recovery in the injured spinal cord of rats. [ABSTRACT FROM AUTHOR]

The aim of this study was to analyze the feasibility of partial replacement of fish meal (FM) with defatted black soldier fly larvae meal (BSFLM) in the diet of Chinese soft-shelled turtles by determining the growth performance, serum biochemical indices, antioxidant capacity, proximate composition, and amino acid profile. The turtles were divided into five groups (G1, G2, G3, G4, and G5) and fed with diets in which 0, 5%, 10%, 15%, and 20% FM was replaced with BSFLM, respectively. The growth performances of the turtles in the G2 and G3 groups were the same as those in the G1 group (P > 0.05), whereas the feed conversion ratio of the G3 group was significantly decreased (P < 0.05). The activity of alkaline phosphatase was significantly higher in the G3 group than that in the G1, G2, and G4 groups (P < 0.05), and the activity of alanine aminotransferase was significantly lower in the G2 and G3 groups than that in the G5 group (P < 0.05). Glutathione peroxidase activity in the serum and total antioxidant capacity in the liver significantly increased in the G3, G4, and G5 groups when compared with the G1 group (P < 0.05), and malonaldehyde levels in the serum and liver showed a linear decrease (P = 0.015 and P = 0.040, respectively) with increasing BSFLM levels in the diets (P < 0.05). The levels of crude protein in the muscle were significantly higher in the G3 and G4 groups than that in the G1 and G2 groups (P < 0.05). The levels of crude fat in the muscle and calipash showed a linear increase (P = 0.000 and P = 0.000, respectively) with increasing BSFLM levels (P < 0.05), whereas the levels of ash showed the opposite trend. Phenylalanine, tryptophan, glutamic, and arginine levels in the muscle and calipash were significantly lower in the other groups than that in the G1 group (P < 0.05), whereas isoleucine level showed the opposite trend. The results showed that the replacement of 10% dietary FM with BSFLM is feasible for Chinese soft-shelled turtles, and it has a positive influence on growth performance, antioxidant capacity, and nutritional value. • BSFLM can replace 10% dietary FM without adverse effects on turtle growth. • 10% and 15% BSFLM diets yielded improved antioxidant capacity and nutritional value. • Replacement of 10% dietary FM with BSFLM is feasible for Chinese soft-shelled turtle. [ABSTRACT FROM AUTHOR]

As the most common pediatric malignancy, B-cell acute lymphoblastic leukemia (B-ALL) has multiple distinct subtypes characterized by recurrent and sporadic somatic and germline genetic alterations. Identification of B-ALL subtypes can facilitate risk stratification and enable tailored therapeutic approaches. Existing methods for B-ALL subtyping primarily depend on immunophenotypic, cytogenetic and genomic analyses, which would be costly, complicated, and laborious in clinical practice applications. To overcome these challenges, we present RanBALL (an Ensemble Ran dom Projection-Based Model for Identifying B -Cell A cute L ymphoblastic L eukemia Subtypes), an accurate and cost-effective model for B-ALL subtype identification based on transcriptomic profiling only. RanBALL leverages random projection (RP) to construct an ensemble of dimension-reduced multi-class support vector machine (SVM) classifiers for B-ALL subtyping. Results based on 100 times 5-fold cross validation tests for >1700 B-ALL patients demonstrated that the proposed model achieved an accuracy of 93.35%, indicating promising prediction capabilities of RanBALL for B-ALL subtyping. The high accuracies of RanBALL suggested that our model could effectively capture underlying patterns of transcriptomic profiling for accurate B-ALL subtype identification. We believe RanBALL will facilitate the discovery of B-ALL subtype-specific marker genes and therapeutic targets, and eventually have consequential positive impacts on downstream risk stratification and tailored treatment design.

This study aimed to investigate the effects of different defatting methods of black soldier fly ( Hermetia illucens ) larvae meal (BSFM) on the metabolic energy and nutrient digestibility in laying hens. Sixty young laying hens (Hy-Line W-36) aged 63 days were randomly divided into two groups (G1 and G2), each with five replicates of six hens housed in individual cages. Group G1 was fed 25% pressed black soldier fly meal (BSFMp) and 75% basal diet, and Group G2 was fed 25% extracted black soldier fly meal (BSFMe) and a 75% basal diet. Both diets included 5 g/kg chromium oxide as an external marker. A 7-day preliminary trial was followed by a 4-day experimental period. The results indicate that pressing and extracting significantly affected the digestibility of crude fat and total energy in BSFM, with BSFMp showing significantly higher crude fat digestibility than BSFMe. Similarly, total energy digestibility was also significantly higher in BSFMp. However, there were no significant differences in dry matter, organic matter, and crude protein digestibility between the two processing methods. The apparent metabolic energy values of BSFMp and BSFMe were 16.34 and 12.41 MJ/kg, respectively, showing a significant difference. The nitrogen-corrected metabolic energy values were 15.89 MJ/kg in BSFMp and 11.93 MJ/kg in BSFMe, indicating a highly significant difference. The digestibility of arginine and leucine in BSFMp was significantly higher than in BSFMe, while differences in lysine, cystine, threonine, tryptophan, and isoleucine were not significant. In conclusion, both defatting methods of BSFM had no adverse effects on the metabolic energy and nutrient digestibility in young laying hens, but BSFMp demonstrated better effects on the digestibility of metabolic energy and nutrients in the feed for young laying hens.

Background: Intracranial pial arteriovenous fistulas (PAVFs) are uncommon neurovascular anomalies that primarily affect the pediatric population. PAVFs are often linked to hereditary hemorrhagic telangiectasia, yet the specific genetic mutations remain unidentified. While endovascular embolization is the preferred treatment for PAVFs, complications like hydrocephalus and sinus thrombosis pose challenges in management., Observations: The authors present a rare case of PAVF in a 6-month-old male neonate with a hereditary GDF2 mutation, where the fistula was supplied by the posterior inferior cerebellar artery and drained directly into the sigmoid sinus. The PAVF was effectively treated with endovascular embolization using coils and Onyx. Furthermore, the authors describe the successful use of rivaroxaban in managing subsequent sinus thrombosis after the embolization of PAVFs. Additionally, the authors review treatment strategies and complications following fistula disconnection., Lessons: Endovascular embolization is the primary treatment choice for the majority of pediatric PAVFs, while a hereditary GDF2 mutation is considered a potential contributing factor to the formation of these malformations in children. Rivaroxaban has shown promise as an effective therapeutic option for pediatric sinus thrombosis, supported by its established safety profile. https://thejns.org/doi/10.3171/CASE24182.

Introduction: Molecular subgroups influence the vascular architecture within medulloblastomas, particularly the wingless (WNT) subgroup, which contributes to its propensity for primary tumor hemorrhage. Whether this mechanism affects intraoperative blood loss remains unknown. This study aimed to assess the association between WNT medulloblastoma and the predisposition for blood loss., Methods: This was a retrospective observational study using data from a neuro-oncology center comprising molecular data on patients treated between December 31, 2014, and April 30, 2023. Differences between WNT and other subgroups in the risk of primary outcome-intraoperative blood loss were assessed using multivariable-adjusted linear regression., Results: Of the 148 patients included in the analysis, 18 patients (12.2%) had WNT, 42 (28.4%) had sonic hedgehog (SHH) TP53 -wildtype, 7 (4.7%) had SHH TP53 -mutant, and 81 (54.7%) were non-WNT/ non-SHH. The WNT subgroup more frequently underwent primary intratumoral hemorrhage (22% vs. 3.8%; p = 0.011). The median intraoperative blood loss was 400.00 (interquartile range [IQR] 250, 500) mL for WNT and 300.00 [200, 400] mL for the other subgroups (p = 0.136), with an adjusted β of 135.264 (95% confidence intervals [CI], 11.701-258.827; p = 0.032). Similar results were observed in both midline and noninfiltrative margin medulloblastoma., Discussion: WNT medulloblastoma is typically associated with primary intratumoral hemorrhage and intraoperative blood loss. The validity of determining the surgical approach based on predicted molecular subtypes from imaging data is questionable. However, attempting to engage in risk communication with patients in a molecular-specific way is worthwhile to validate., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zhang, Wu, Zhao, Ji, Li, Zhai, Liang, Cheng and Zhou.)

Objective: The study aimed to explore the association between the site of interictal epileptic discharges (IEDs) on postoperative electroencephalogram (EEG) and seizure recurrence after antiepileptic drug (AED) withdrawal. The study hypothesizes that the concordance of IED sites with surgical sites indicates incomplete resection of epileptic focus, while non-concordance of IED sites with surgical sites indicates postoperative changes or cortical stimulation. The former has a higher risk of seizure recurrence., Methods: We retrospectively analyzed the postoperative EEG pattern of 182 consecutive children who underwent resection surgery. To identify the risk factors for seizure recurrence, we compared the attributes of seizure recurred and seizure-free groups by univariate and multivariate analyses. AED tapering was standardized, involving a 25% reduction in the dose of a single type of AED every 2 weeks, independent of the presurgical AED load., Results: We attempted AED withdrawal in 116 (63.7%) children. Twenty-eight (24.1%) children experienced seizure recurrence during or after AED withdrawal. A greater number of AEDs used at the time of surgery ( p =0.005), incomplete resection ( p =0.001), and presence of IED on postoperative EEG ( p =0.011) are predictors of seizure recurrence. The completeness of resection and seizure recurrence after AED withdrawal were related to the presence of IED on the EEG, but not to the concordance of IED with surgical sites., Conclusion: For children with abnormal EEG, the decision to discontinue AED should be made more cautiously, regardless of the relative location of the discharge site and the surgical site., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY. Published by BMJ.)

With significant advancements of next generation sequencing technologies, large amounts of multi-omics data, including genomics, epigenomics, transcriptomics, proteomics, and metabolomics, have been accumulated, offering an unprecedented opportunity to explore the heterogeneity and complexity of cancer across various molecular levels and scales. One of the promising aspects of multi-omics lies in its capacity to offer a holistic view of the biological networks and pathways underpinning cancer, facilitating a deeper understanding of its development, progression, and response to treatment. However, the exponential growth of data generated by multi-omics studies present significant analytical challenges. Processing, analyzing, integrating, and interpreting these multi-omics datasets to extract meaningful insights is an ambitious task that stands at the forefront of current cancer research. The application of artificial intelligence (AI) has emerged as a powerful solution to these challenges, demonstrating exceptional capabilities in deciphering complex patterns and extracting valuable information from large-scale, intricate omics datasets. This review delves into the synergy of AI and multi-omics, highlighting its revolutionary impact on oncology. We dissect how this confluence is reshaping the landscape of cancer research and clinical practice, particularly in the realms of early detection, diagnosis, prognosis, treatment and pathology. Additionally, we elaborate the latest AI methods for multi-omics integration to provide a comprehensive insight of the complex biological mechanisms and inherent heterogeneity of cancer. Finally, we discuss the current challenges of data harmonization, algorithm interpretability, and ethical considerations. Addressing these challenges necessitates a multidisciplinary collaboration, paving the promising way for more precise, personalized, and effective treatments for cancer patients., (Copyright © 2024. Published by Elsevier Inc.)

Purpose: To construct a machine learning model based on radiomics of multiparametric magnetic resonance imaging (MRI) combined with clinical parameters for predicting Sonic Hedgehog (SHH) and Group 4 (G4) molecular subtypes of pediatric medulloblastoma (MB)., Methods: The preoperative MRI images and clinical data of 95 patients with MB were retrospectively analyzed, including 47 cases of SHH subtype and 48 cases of G4 subtype. Radiomic features were extracted from T1-weighted imaging (T1), contrast-enhanced T1 weighted imaging (T1c), T2-weighted imaging (T2), T2 fluid-attenuated inversion recovery imaging (T2FLAIR), and apparent diffusion coefficient (ADC) maps, using variance thresholding, SelectKBest, and Least Absolute Shrinkage and Selection Operator (LASSO) regression algorithms. The optimal features were filtered using LASSO regression, and a logistic regression (LR) algorithm was used to build a machine learning model. The receiver operator characteristic (ROC) curve was plotted to evaluate the prediction accuracy, and verified by its calibration, decision and nomogram. The Delong test was used to compare the differences between different models., Results: A total of 17 optimal features, with non-redundancy and high correlation, were selected from 7,045 radiomics features, and used to build an LR model. The model showed a classification accuracy with an under the curve (AUC) of 0.960 (95% CI: 0.871-1.000) in the training cohort and 0.751 (95% CI: 0.587-0.915) in the testing cohort, respectively. The location of the tumor, pathological type, and hydrocephalus status of the two subtypes of patients differed significantly ( p < 0.05). When combining radiomics features and clinical parameters to construct the combined prediction model, the AUC improved to 0.965 (95% CI: 0.898-1.000) in the training cohort and 0.849 (95% CI: 0.695-1.000) in the testing cohort, respectively. There was a significant difference in the prediction accuracy, as measured by AUC, between the testing cohorts of the two prediction models, which was confirmed by Delong's test ( p = 0.0144). Decision curves and nomogram further validate that the combined model can achieve net benefits in clinical work., Conclusion: The combined prediction model, constructed based on radiomics of multiparametric MRI and clinical parameters can potentially provide a non-invasive clinical approach to predict SHH and G4 molecular subtypes of MB preoperatively., Competing Interests: YJ was employed by Huiying Medical Technology Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Wang, Wang, Qin, Hu, Xiao, Tong, Li, Jing, Li and Zhang.)

Aim: To construct a prediction nomogram model for the postoperative seizure outcomes in children with focal cortical dysplasia (FCD)., Material and Methods: We retrospectively reviewed the clinical data of 97 children with epilepsy secondary to FCD who had undergone resection surgery at Children's Hospital of Chongqing Medical University from June 2013 to September 2019. Univariate and multivariate Cox proportional hazards regression were used to explore the predictors of postoperative persistent seizure, and a nomogram prediction model for postoperative seizure outcome was developed. The C-index was chosen to evaluate the discriminability of the nomogram with internal validation. Calibration curves and decision curve analysis were used to evaluate consistency and clinical efficacy, respectively., Results: The complete resection of epileptogenic focus and the pathological type of FCD were independent predictors of persistent seizure in children with epilepsy secondary to FCD after surgery. Based on multivariate Cox proportional hazard regression, a predictive nomogram for epilepsy outcome was established and validated via the bootstrap method with 1000 resamples. The nomogram showed superior prediction accuracy (C-index = 0.883); by drawing and reviewing the calibration curve and decision curve, the nomogram presented good consistency and clinical efficacy., Conclusion: A nomogram prediction model of postsurgery seizure outcome in children with epilepsy secondary to FCD was constructed based on four variables, providing a reliable and convenient tool for individual seizure outcome prediction.

The aim of the present study was to investigate the effects of dietary supplementation with different doses of rosmarinic acid (RA) on the growth performance, serum biochemical indices, liver antioxidant activity, and muscle nutritional component of broiler chickens during 1-20 days of age. A total of 1000 1-day-old Cobb 500 white-feathered broilers were divided into five groups. Each group included four replicates and 50 birds per replicate. The control group was the basic fodder group fed with a basal diet. The experimental groups included four levels of RA (100, 200, 300, and 400 mg/kg RA added to the basal diet, respectively). The results showed that a quadratic increase in final body weight (BW) and average daily gain (ADG) and a quadratic decrease in the feed/gain (F/G) ratio were observed with increasing dietary RA levels. At 200 mg/kg RA supplementation, growth performance was significantly improved. Moreover, under this RA treatment, the highest levels of total protein and globulin were detected in the serum, the activities of total superoxide dismutase and catalase in the liver of broilers were significantly increased, and the malondialdehyde content was significantly decreased, indicating a higher antioxidant capacity of the liver when 200 mg/kg RA was added to the basal diet. The flavor of the muscle meat was improved by dietary supplementation with 200 mg/kg RA. Therefore, adding 200 mg/kg of RA to the diet could significantly improve the growth performance of broilers, improve liver antioxidant capacity, and improve muscle quality, etc. The addition of RA to broiler feed as a natural antioxidant has great prospects.

Substitution of feed protein source with defatted black soldier fly larvae meal (BSFM) has been evaluated intensively in poultry, but information about full-fatted BSFM is still very limited. The aim of the present study was to investigate the effect of dietary low inclusion of full-fatted BSFM on the growth performance, plasma antioxidant ability, nutrient digestibility, and gut health of layer chickens during 1-42 days of age. A total of 480 female 1-day-old Hy-Line Brown chickens were divided into four dietary treatments, with the inclusion of 0, 3, 6, and 9% of full-fatted BSFM. Each treatment included six replicates and 20 birds per replicate. As dietary full-fatted BSFM inclusion levels increased, there was a quadratic increase in final weight and average daily gain and a quadratic decrease in feed/gain ratio. Dietary full-fatted BSFM inclusion levels increased the digestibility of crude protein and ether extract quadratically as well as ileum mucosal sIgA concentration linearly, but these had no effect on intestinal morphology. Additionally, an increase in dietary full-fatted BSFM inclusion levels resulted in a linear increase in glutathione peroxidase and total superoxide dismutase activities and a linear decrease in malondialdehyde content in plasma. The encouraging results of the improvement of growth performance, nutrient digestibility, antioxidant ability, and gut health parameters suggested that partially full-fatted BSFM inclusion can be suitable protein ingredients for layer chickens' diets at the starter period., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2020 Chu, Li, Wang, Wang, Shang, Wang and Li.)

We herein report two cases of post-treatment maturation of medulloblastoma (MB). We also conducted a literature review to summarize the clinical and pathologic features of MB maturation. From January 1992 to February 2017, 52 patients with MB underwent surgical resection followed by radiotherapy and chemotherapy. Tumor cell maturation was identified in two patients who underwent a second surgery. We conducted a systematic search of PubMed and identified six such cases. In both of our patients, the pathologic type was MB with extensive nodularity (MBEN). Both patients underwent radiotherapy and chemotherapy. The tumor differentiated to gangliocytoma in both patients. In the overall analysis that also included the six cases identified in the literature, the pathologic types were classic MB (n=1), desmoplastic/nodular MB (n=2), MBEN (n=3), and unclassified MB (n=2). MB differentiated into the following types: gangliocytoma (n=2), ganglioglioma (n=1), melanocyte (n=1), neuronal differentiation (n=2), and classic MB (n=1). Desmoplastic/nodular MB and MBEN can differentiate into less malignant cells types after radiotherapy and chemotherapy. Maturation of MB may be affected radiotherapy and chemotherapy.

Aim: To identify differentially expressed microRNAs in medulloblastoma, and to investigate their biological function., Material and Methods: Differentially expressed microRNAs were identified using gene chips, and significantly different microRNAs were selected for verification using real time quantitative PCR. Potential target genes and their biological pathways were predicted by bioinformatics software., Results: Our analysis identified two microRNAs, hsa-miR-208a-3p and hsa-miR-1207-5p, which were significantly downregulated in medulloblastoma. Bioinformatics analysis identified potential target genes in the Wnt and MAPK signaling pathways, including NLK, RAPGEF2, CACNA2D1, DUSP3, MAPK8IP3., Conclusion: Downregulation of hsa-miR-208a-3p and hsa-miR-1207-5p may be involved in the occurrence of medulloblastoma, through modulations of the Wnt and MAPK signaling pathways.