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714 results on '"Li, Yuzhen"'

2. Expert Consensus on Big Data Collection of Skin and Appendage Disease Phenotypes in Chinese

Author

Zhao, Shuang, Luo, Zhongling, Wang, Ying, Gao, Xinghua, Tao, Juan, Cui, Yong, Chen, Aijun, Cai, Daxing, Ding, Yan, Gu, Heng, Gu, Jianying, Ji, Chao, Kang, Xiaojing, Lu, Qianjin, Lv, Chengzhi, Li, Min, Li, Wei, Liu, Wei, Li, Xia, Li, Yuzhen, Man, Xiaoyong, Qiao, Jianjun, Sun, Liangdan, Shi, Yuling, Wu, Wenyu, Xia, Jianxin, Xiao, Rong, Yang, Bin, Kuang, Yehong, Chen, Zeyu, Fang, Jingyue, Kang, Jian, Yang, Minghui, Zhang, Mi, Su, Juan, Zhang, Xuejun, and Chen, Xiang

Published
2024
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12. Effect of Metal-Binding Antimicrobial Peptide SIF4 on Topoisomerases Activity and Intracellular Nucleic Acid Biosynthesis in Escherichia coli

Author

LI Yuzhen, XIAO Huaiqiu, ZHOU Huiheng, LI Lan, KUANG Yan, LIU Miao, ZHAO Mouming

Subjects
metal-binding antimicrobial peptide, escherichia coli, topoisomerase, nucleic acid, antimicrobial agent, Food processing and manufacture, TP368-456
Abstract

To systematically elucidate how metal-binding antimicrobial peptide SIF4 exerts its antimicrobial activity by targeting DNA topoisomerase without destroying the cytoplasmic membrane, Escherichia coli was used as a model strain to investigate the binding mode of SIF4 with its genomic DNA and the impact of SIF4 on DNA topoisomerase I and II activities and intracellular nucleic acid biosynthesis. Results showed that SIF4 could bind to genomic DNA in a manner similar to ethidium bromide (EB) intercalation with strong inhibitory effect on topoisomerase I but weak effect on topoisomerase II, and catalyzed RNA transcription to exert antimicrobial activity by interfering with the unwinding of negative DNA supercoils and RNA polymerase binding. It was also found that the biosynthesis of intracellular DNA and RNA was inhibited to different degrees after 12 h treatment with SIF4, which exhibited a good dose-effect relationship. There was no significant difference in the amounts of intracellular DNA and RNA between the 1/2 minimum inhibitory concentration (MIC) group and the control group (P > 0.05), but there was a significant difference between the MIC and 2 MIC groups and the control group (P < 0.05). Our results may provide theoretical support for the application of SIF4 in the biocontrol of foodborne E. coli.

Published
2024
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13. Metal-Binding Antimicrobial Peptide SIF4 Kills Escherichia coli by Targeting Cytoplasmic Biomacromolecules without Cytoplasmic Membrane Damage: A Mechanistic Study

Author

LI Yuzhen, XIAO Huaiqiu, LIU Miao, WANG Lin, ZENG Mengqi, ZHAO Mouming

Subjects
metal antimicrobial peptide, escherichia coli, intracellular biomacromolecules, non-cytoplasmic membrane damage, antimicrobial mechanism, Food processing and manufacture, TP368-456
Abstract

To explore how metal-binding antimicrobial peptide SIF4 kills foodborne Escherichia coli by targeting nucleic acid and protein in the cytoplasmic membrane without cytoplasmic membrane damage, the effect of SIF4 on intracellular nucleic acid biosynthesis was investigated, and fluorescence spectral analysis of the competition between SIF4 and ethidium bromide (EB) for binding to genomic DNA, ultraviolet (UV) spectral analysis of the interaction between SIF4 and genomic DNA, and the binding mode between SIF4 and genomic DNA were studied. Besides, the effect of SIF4 on intracellular protein biosynthesis was systematically evaluated. Results demonstrated that SIF4 could bind to E. coli genomic DNA through groove insertion and inhibit dose-dependently nucleic acid biosynthesis. Fluorescence spectral analysis showed that SIF4 could compete with EB for binding to genomic DNA through intercalation binding and electrostatic adsorption. UV spectroscopy showed that combination with SIF4 changed the molecular conformation of genomic DNA, but did not break its double strand structure. Circular dichroism (CD) spectroscopy showed that the base stacking force of genomic DNA was weakened, the double helix structure became loose, and the genomic DNA structure was changed from B to C configuration after combination with SIF4. In addition, SIF4 could significantly affect intracellular protein biosynthesis, and its inhibition effect was positively correlated with the treatment time and dose of SIF4. It is believed that SIF4 can enter the DNA groove through electrostatic adsorption or intercalation with genomic DNA, affecting DNA replication, RNA transcriptional biomass and protein translation to produce antimicrobial effect against Escherichia coli without cytoplasmic membrane damage. These results can provide support for the biocontrol of foodborne E. coli.

Published
2023
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14. Serum dsDNA is environmentally contingent

Author

Zhen, Qi, Chen, Weiwei, Han, Yang, Wang, Yirui, Li, Zhuo, Qu, Guangbo, Ge, Huiyao, Li, Bao, Mao, Yiwen, Yu, Yafen, Bai, Bingxue, Lv, Chengzhi, Zhang, Jin, Hu, Huaqing, Jiang, Qijun, Kang, Xiaojing, Xu, Yuanhong, Lu, Yan, Zhao, Juan, Wu, Song, Li, Shijie, Chen, Xia, Qi, Ruiqun, Lin, Xiaohong, Han, Jianwen, Lu, Yonghong, Shi, Jihai, Qiu, Ying, Fan, Yiming, Li, Shanshan, Li, Fuqiu, Li, Yuzhen, Gao, XingHua, and Sun, Liangdan

Published
2024
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23. Inhibitory Mechanism of Metal-Binding Antimicrobial Peptide SIF4 on Respiratory and Energy Metabolism of Escherichia coli

Author

LI Yuzhen, XIAO Huaiqiu, LIU Miao, WANG Lin, ZENG Mengqi, ZHAO Mouming

Subjects
escherichia coli, metal-binding antimicrobial peptide, respiratory metabolic pathway, energy metabolism, antimicrobial mechanism, Food processing and manufacture, TP368-456
Abstract

This study aimed to systematically elaborate on the inhibitory mechanism of metal-binding antimicrobial peptide SIF4 on the respiratory and energy metabolism of Escherichia coli. By analyzing changes in the metabolic activity, individual and synergistic respiratory inhibition rate, cytoplasmic membrane ion channel ATPase and intracellular ATP level of E. coli after being treated with SIF4, the effects of SIF4 on the cell metabolic activity, respiratory metabolic pathway, cytoplasmic membrane ion channel ATPase and intracellular ATP biosynthesis were studied. Results showed that the metabolic activity of E. coli decreased significantly with increasing dose of SIF4 (P < 0.05), and decreased by 70.41% in the 2 × minimal inhibitory concentration (MIC) group compared with the control group. SIF4 had good inhibitory effect on the respiration of Escherichia coli, with an inhibition rate of (19.387 ± 0.168)% and (25.222 ± 0.326)% at MIC and 2 × MIC, respectively. The synergistic respiratory inhibition rate of SIF4 combined with iodoacetic acid was the lowest ((19.982 ± 0.133)%), indicating that SIF4 could exhibit high antimicrobial activity mainly by inhibiting the glycolysis pathway of E. coli. The activities of cytoplasmic membrane ion channel Na+K+-ATPase and Ca2+Mg2+-ATPase decreased after treatment with SIF4, and this effect was positively correlated with SIF4 dose and treatment time, but weaker than that of the positive control Triton X-100. As SIF4 dose and treatment time increased, the intracellular ATP concentration decreased significantly, and after 12 h, the intracellular ATP concentration in the 2 × MIC group was significantly lower than that in the control group but higher than that in the positive control group (P < 0.05). All results confirmed that SIF4 could exhibit high antimicrobial activity against E. coli by interfering with respiratory metabolism, weakening cytoplasmic membrane ion channel ATPase activity and inhibiting the biosynthesis of intracellular ATP, which can provide theoretical support for the biocontrol of foodborne E. coli.

Published
2023
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42. Hemoporfin-mediated photodynamic therapy for the treatment of port-wine stain: A multicenter, retrospective study

Author

Zhang, Xiaofeng, Yuan, Chen, Xiao, Xuemin, Yin, Rui, Lei, Hongzhao, Li, Yan, Zheng, Shumao, Wen, Sijian, Li, Dongsheng, Wang, Xuejun, Lu, Zhong, Zhang, Yunfeng, Zeng, Weihui, He, Sijin, Li, Yuzhen, Jian, Dan, Yang, Jun, Zhong, Hua, Han, Dawei, Chen, Xiaoying, Zhou, Junfeng, Cai, Yantao, Peng, Xi, Li, Zhiming, Liu, Xueying, Lin, Tong, Zhang, Ruzhi, Li, Guang, Zhuang, Yin, Liu, Ling, Yan, Yan, and Wang, Baoxi

Published
2023
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48. GWAS of Chronic Spontaneous Urticaria Reveals Genetic Overlap with Autoimmune Diseases, Not Atopic Diseases

Author

Zhang, Liming, Qiu, Li, Wu, Jian, Qi, Yumeng, Gao, Xinghua, He, Chundi, Qi, Ruiqun, Wang, Hexiao, Yao, Xu, Zhu, Hong, Li, Yuzhen, Hao, Siyu, Lu, Qianjin, Long, Hai, Lian, Shi, Zhu, Wei, Zhang, Haiping, Lai, Wei, Su, Xiangyang, Lu, Rongbiao, Guo, Zaipei, Li, Jingyi, Li, Mengmeng, Liu, Quanzhong, Wang, Huiping, He, Li, Nong, Xiang, Li, Fuqiu, Li, Yang, Yao, Chunli, Xu, Jinhua, Tang, Hui, Wang, Duoqin, Li, Zhenlu, Yu, Huiqian, Xiao, Shengxiang, An, Jingang, Pu, Xiongming, Yu, Shirong, Zhang, Jianzhong, Chen, Xue, Wang, Haifeng, Huang, Wei, Chen, Hongduo, and Xiao, Ting

Published
2023
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