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The presence of multiple donor‐specific antibodies (DSAs) targeting HLA antigens poses a challenge to transplantation. Various techniques, including the use of recombinant cell lines and crossmatch cells have been developed to isolate DSAs. To simplify the extraction of HLA‐specific DSAs from complex sera, we introduced magnetic beads with single HLA specificity (MagSort). Sera were treated with MagSort, allowing HLA‐specific antibodies to bind to the beads, and these specific antibodies were subsequently eluted. MagSort beads, coated with 59 different HLA variants, underwent testing through 1329 adsorption/elution processes, demonstrating their effectiveness and specificity in adsorbing and eluting HLA‐specific antibodies. The MagSort method proves comparable to the cell method, showing similar isolated antibody binding patterns. The isolated antibody binding patterns from MagSort reveal both known eplets and unknown patterns, suggesting its utility for eplet discovery. Additionally, MagSort proved effective in extracting signals for flow cytometry cross‐matching, offering a means to assess the binding capability of isolated antibodies against specific donor cells. [ABSTRACT FROM AUTHOR]

This small qualitative study explored the dimensions and stages of development of the professional identity of school counsellors over the span of their careers. Study participants examined experiences that demonstrated their identity as professional school counsellors. Four dimensions were found to be essential to having a strong professional identity: being highly committed to working with students; fulfilling appropriate roles; increasing competence; and associating with other professional counsellors. Development of each dimension consists of knowing about it, valuing it and acting from that base. With more than 100 years of school counselling experience among them, the participants learned that, although their journeys were different, they experienced similar developmental stages. Highlights of their experiences illustrate the stages within each dimension. [ABSTRACT FROM AUTHOR]