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Based on big data, a new public space evaluation method is proposed. Using programming technology to collect visitor reviews from the travel website TripAdvisor to build a database, based on the data of 99,240 words in 1573 visitor reviews in 10 years, the connection between data and reality is established through systematic data classification and visualization. Following an assessment of the Kimbell Art Museum's functionality, architectural design, and landscape design, along with visitor feedback, a new evaluation methodology was formulated for application to public buildings with landscapes. By utilizing the unique advantages of big data, it provides convenient and efficient analysis methods for public spaces with similar data foundations and opens the way for the optimization of the built environment in the information age. [ABSTRACT FROM AUTHOR]

Background: Exosomes are vesicles secreted by all types of mammalian cells. They are characterized by a double‐layered lipid membrane structure. They serve as carriers for a plethora of signal molecules, including DNA, RNA, proteins, and lipids. Their unique capability of effortlessly crossing the blood–brain barrier underscores their critical role in the progression of various neurological disorders. This includes, but is not limited to, diseases such as Alzheimer's, Parkinson's, and ischemic stroke. Establishing stable and mature methods for isolating exosomes is a prerequisite for the study of exosomes and their biomedical significance. The extraction technologies of exosomes include differential centrifugation, density gradient centrifugation, size exclusion chromatography, ultrafiltration, polymer coprecipitation, immunoaffinity capture, microfluidic, and so forth. Each extraction technology has its own advantages and disadvantages, and the extraction standards of exosomes have not been unified internationally. Aims: This review aimed to showcase the recent advancements in exosome isolation techniques and thoroughly compare the advantages and disadvantages of different methods. Furthermore, the significant research progress made in using exosomes for diagnosing and treating central nervous system (CNS) diseases has been emphasized. Conclusion: The varying isolation methods result in differences in the concentration, purity, and size of exosomes. The efficient separation of exosomes facilitates their widespread application, particularly in the diagnosis and treatment of CNS diseases. [ABSTRACT FROM AUTHOR]

The accurate determination of Atmospheric Boundary Layer Height (ABLH) is crucial in various atmospheric studies and practical applications. In this study, we present a comprehensive comparative analysis of five distinct methods for estimating ABLH using Global Navigation Satellite System (GNSS) Radio Occultation (RO) data. These methods encompass the use of bending angle and refractivity profiles, namely Minimum Gradient methods of the Bending Angle (MGBA) and Refractivity (MGR) profiles, breaking point, Wavelet Covariance Transform (WCT), and Double-Parameter Model Function (DPMF). GNSS-RO data from COSMIC-2 and Spire are used. To establish robust validation, radiosonde data are employed as a reference, ensuring the reliability of our findings. The results reveal notable variations in the performances of these ABLH estimation methods. Specifically, the MGBA, MGR, breaking point, and DPMF methods exhibit strong correlations with the reference data. Conversely, the WCT method displays weaker correlations, higher biases, and elevated root-mean-square-errors, suggesting limitations in capturing the true ABLH. Furthermore, we remove outlier screening to facilitate a comparison of the differences among the five methods. The WCT and DPMF methods can detect strong variations in the profiles near the Earth's surface and consider them as ABLH. However, these variations are caused by errors. The MGBA method emerges as a reliable and stable option, while the WCT and DPMF methods should be used with caution due to the lower quality of the GNSS-RO profiles near the Earth's surface. [ABSTRACT FROM AUTHOR]

OBJECTIVES The purpose of this retrospective study was to summarize our experience in performing robot-assisted thoracoscopic surgery (RATS) for mediastinal tumours in children to investigate its safety and feasibility. METHODS This retrospective study involved 149 patients with mediastinal tumours who were hospitalized in the Department of Thoracic Surgery of Beijing Children's Hospital, Capital Medical University, and underwent RATS for tumour resection from March 2021 to November 2022. Information on patient age, weight, tumour size, surgical incision selection, operative time, intraoperative bleeding, intraoperative complications, length of hospital stay, rate of conversion to thoracotomy and follow-up conditions were summarized. RESULTS All 149 surgeries were successfully completed with no cases of mortality. There were 77 male and 72 female patients, with a mean age of 5.9 years (range: 6 months–16 years, 8 months) and a mean weight of 23.6 kg (8.0–72.0 kg). The mean maximum tumour diameter was 5.5 cm (2.0–12.0 cm), the mean operative time was 106.7 min (25.0–260.0 min), the mean intraoperative bleeding volume was 11.3 ml (1.0–400.0 ml) and the mean hospital stay was 7.2 days (4.0–14.0 days). All patients recovered well with no cases of tumour recurrence or mortality during the postoperative follow-up period (3–23 months). CONCLUSIONS RATS is safe and feasible to apply in children with mediastinal tumours who are >6 months of age and weigh more than 8 kg in terms of short-term outcomes, but longer-term follow-up is needed to fully evaluate the benefits. For cases that are associated with greater surgical difficulty and risk, a comprehensive surgical plan should be fully prepared in advance of surgery. [ABSTRACT FROM AUTHOR]

In this study, we investigate the performances of a commercial Global Navigation Satellite System (GNSS) Radio Occultation (RO) mission and a new-generation RO constellation, i.e., Spire and Constellation Observing System for Meteorology, Ionosphere, and Climate 2 (COSMIC-2), respectively. In the statistical comparison between Spire and COSMIC-2, the results indicate that although the average signal-to-noise ratio (SNR) of Spire is far weaker than that of COSMIC-2, the penetration of Spire is comparable to, and occasionally even better than, that of COSMIC-2. In our analysis, we find that the penetration depth is contingent upon various factors including SNR, GNSS, RO modes, topography, and latitude. With the reanalysis of the European Centre for Medium-Range Weather Forecasts and Radiosonde as the reference data, the identical error characteristics of Spire and COSMIC-2 reveal that overall, the accuracy of Spire's neutral-atmosphere data products was found to be comparable to that of COSMIC-2. [ABSTRACT FROM AUTHOR]

Background: Leucine-rich repeat-containing protein-25 (LRRC25) can degrade the ISG15 gene in virus-infected cells and prevent overactivation of the type Ⅰ IFN pathway. However, the role of LRRC25 in bacterial infection is still unclear. In this pursuit, the present study aimed to explore the regulatory role and mechanism of LRRC25 in microglia infected with Mycobacterium tuberculosis in a mouse model. Methods: Q-PCR, WB, and cell immunofluorescence were employed to observe the change in LRRC25 in BV2 cells infected by H37Rv. Additionally, siRNA was designed to target the LRRC25 to inhibit its expression in BV2 cells. Flow cytometry and laser confocal imaging were used to observe the infection of BV2 cells after LRRC25 silencing. Q-PCR and ELISA were used to determine the changes in IFN-γ and ISG15 in the culture supernatant of each group. Results: Following H37Rv infection, it was observed that the expression of LRRC25 was upregulated. Upon silencing LRRC25, the proportion of BV2 cells infected by H37Rv decreased significantly. ELISA analysis showed that IFN-γ and ISG15 levels in cell culture supernatant decreased after H37Rv infection, while they significantly increased after LRRC25 silencing. Conclusions: This study provides evidence that LRRC25 is the key negative regulator of microglial anti-Mtb immunity. It exerts its function by degrading free ISG15 and inhibiting the secretion of IFN-γ, thereby improving the anti-Mtb immunity of BV2 cells. [ABSTRACT FROM AUTHOR]

In modern cities, urban public spaces, such as parks, gardens, plazas, and streets, play a big role in people's social activities, physical activities, mental health, and overall well-being. However, the traditional post-occupancy evaluation (POE) process for public spaces such as large urban parks is extremely difficult, especially for long-term user experiences through observations, surveys, and interviews. On the other hand, social media has emerged as a major media outlet recording millions of user experiences to the public, which provides opportunities to inform how public space is used and perceived by users. Furthermore, unlike previous research that primarily presented descriptive characters of park programs, our study employs a network model to elucidate the interactive relationships and intensities among reported park elements, human activities, and experiences. This approach enables us to track the sources within the space that impact people's perceptions, such as weather conditions, food options, and notable landmarks. The utilization of this network model opens avenues for future research to comprehensively investigate the factors shaping people's perceptions in public open spaces. This study uses Bryant Park as an example and presents a new analytical framework, POSE (post-occupancy social media evaluation), to support long-term POE studies for large public spaces. Methods such as data automation, descriptive statistics, and social network analysis were used. The identification and quantification of meaningful park activities, scenes, and sentiments as well as their relationships will help optimize the design and management of park programs. [ABSTRACT FROM AUTHOR]

Coastal communities face escalating risks from rising sea levels and the increasing growth of vulnerable, aging populations in high-risk zones. These threats are expected to intensify as population growth and aging trends continue. In response to these challenges, this study represents a novel investigation into the synergistic impacts of demographic shifts and climate change in shaping the vulnerability of coastal communities, particularly focusing on elderly populations. This study's primary objectives are to assess the potential impacts of these threats on vulnerable older adults and to explore effective adaptation strategies. To achieve these objectives, we used census tract data from Hawaii and the Hamilton–Perry cohort-component method to project the elderly population trends in each census tract for Honolulu in 2050. The vulnerabilities of older adults were estimated under different sea level rise level conditions and mapped according to three planning scenarios: (1) maintaining the status quo; (2) relocating or redeveloping vulnerable elderly residents to safer, low-density neighborhoods; (3) relocating or redeveloping vulnerable elderly residents to secure, high-density areas with amenities for older adults. We further evaluated transportation accessibility to emergency services in these scenarios. The findings reveal that with a projected sea level rise of 1.1 feet, the number of elderly individuals without timely access (within 8 min) to emergency and healthcare services would double by 2050. This is primarily attributed to reduced transportation access and increased aging in high-risk areas. Compared to the status quo, both relocation (or redevelopment) strategies significantly improve the vulnerable elderly population's access to emergency and healthcare services, even without enhancements in transportation and infrastructure. Given that many developments and aging trends are yet to fully unfold, we propose that existing adaptation strategies should prioritize land use development, along with housing and transportation solutions that align with development scenarios 2 and 3, to support age-friendly activities and lifestyles. By directing population growth towards less vulnerable zones in the coming decades, we can achieve protective effects equivalent to those of future relocation efforts, but without incurring substantial protection or relocation costs. [ABSTRACT FROM AUTHOR]

The aim of the study was to analyze population-based prostate cancer (PCa) screening and the incidence of PCa among males ≥50 years of age residing in the Luqiao district of Taizhou, China. From October to December 2020, male residents ≥50 years of age were screened for serum total prostate-specific antigen (total-PSA). If t-PSA re-test levels persisted above 4 μg/L, subjects underwent further noninvasive examinations, including digital rectal examination or multiparameter magnetic resonance imaging (mpMRI) of the prostate. Subjects underwent prostate biopsy of pathological tissue based on t-PSA and mpMRI results. A total of 3524 (49.1%) residents participated in this PCa screening study. In total, 285 (8.1%) subjects exhibited t-PSA levels ≥4.0 μg/L and 112 (3.2%) underwent noninvasive examinations. Forty-two (1.2%) residents underwent prostate biopsy, of which 16 (0.45%) were diagnosed with PCa. Of those diagnosed with PCa, three (19%) had localized PCa (cT1-cT2N0M0), six (37%) had locally advanced PCa (cT3a- cT4N0-1M0), and seven (44%) had advanced metastatic PCa (M1). Unfortunately, 3477 (48.5%) residents did not participate in the study, mainly due to lack of awareness of PCa based on feedback from local health centers. Age and t-PSA were used as primary screening indicators and, when further combined with mpMRI and prostate biopsy, confirmed the diagnosis of PCa among participating residents. Although this was a relatively economical and convenient screening method, education and knowledge should be further enhanced to increase the participation rate in PCa screening programs. [ABSTRACT FROM AUTHOR]

Importance: Pulmonary inflammatory myofibroblastic tumors (PIMTs) are primary lung tumors in children. Misdiagnosis easily occurs because of the nonspecific clinical manifestations, laboratory examination results, and imaging findings in affected patients. Objective: To summarize the clinical characteristics, diagnosis, and prognosis of children with PIMTs. Methods: This retrospective analysis included 23 children with PIMTs who received treatment in our hospital from January 2008 to January 2019. The clinical manifestations, laboratory examination results, and computed tomography findings were retrospectively analyzed. Results: The population included 13 boys and 10 girls, with a median age at onset of 78 months (range, 10–126 months). Fourteen patients had PIMT lesions in the right lung and nine patients had PIMT lesions in the left lung. The surgical procedures included pulmonary wedge resection, pulmonary lobectomy, and total pneumonectomy. The median operation time was 115 min (range, 45–235 min); the median intraoperative blood loss volume was 30 mL (range, 3–500 mL). During the operation, one patient each had pulmonary hemorrhage, vena cava hemorrhage, and thoracic duct injury. Postoperative complications included pulmonary embolism in one patient and tumor recurrence in two patients; neither of these complications recurred after reoperation. The median follow‐up period was 49 months (range, 2–127 months). Interpretation: Children with PIMTs exhibited good prognoses and primarily underwent surgical resection as treatment. Complete preoperative evaluation should be performed in affected patients. [ABSTRACT FROM AUTHOR]

Background: A retrospective study was carried out to determine the feasibility and safety of an extracorporeal-assisted intracorporeal sliding knot-tying technique in minimally invasive surgery in children. Materials and Methods: From June 2009 to December 2017, a total of 333 cases of pediatric minimally invasive surgery were performed using the extracorporeal-assisted intracorporeal sliding knot-tying technique. Polyester, polyglactin, and polydioxanone sutures were used for suturing and knotting. The average time used for knotting was recorded during the surgery. The patients were followed up for unraveled knots and recurrence of the diseases. Results: All 333 surgical procedures were performed successfully, including 152 cases of thoracoscopic diaphragmatic hernia repair, 151 cases of thoracoscopic diaphragmatic plication, 7 cases of thoracoscopic esophageal anastomosis, and 23 cases of laparoscopic esophageal hiatal hernia fundoplication. No serious complications or mortalities were observed. Twelve-month to 9-year follow-up showed that all pediatric patients recovered well and no recurrence or unraveled knots were found. Conclusions: This new knot-tying technique is safe and feasible for various minimally invasive endoscopic surgeries, especially for suturing tissues under tension, such as thoracoscopic diaphragmatic hernia repair, laparoscopic esophageal hiatal hernia repair, and fundoplication. All types of sutures can be used for this knot-tying technique. It is easy to learn and can be quickly mastered by doctors with endoscopic surgery experience. [ABSTRACT FROM AUTHOR]

ΔNp63αplays key roles in cell survival and proliferation. So its expression is always tightly controlled in cells. We previously reported that DNA damage down-regulates transcription of ΔNp63αin FaDu and HaCat cells, which contributes to cell apoptosis. In the present study, we found that DNA damage induces down-regulation of ΔNp63αvia facilitating its proteasomal degradation in cell lines such as MDA-MB-231 and MCF10A. Further investigation revealed that transcription of WWP1 is stimulated by DNA damage in these cells. Knock-down of WWP1 abrogates DNA damage-induced down-regulation of ΔNp63αand partially rescues cell apoptosis. Interestingly, DNA damage may stimulate WWP1 through different mechanisms in different cell types: it up-regulates transcription of WWP1 in a p53-dependent manner in MCF10A and HEK293 cells, while miR-452 may be involved in DNA damage-induced up-regulation of WWP1 in MDA-MB-231 cells. Our study demonstrates a novel pathway which regulates ΔNp63αupon cellular response to chemotherapeutic agents. [ABSTRACT FROM AUTHOR]

TAp63γ is a homologue of tumor suppressor p53 and functions as a transcriptional factor playing key roles in cell cycle and cell apoptosis. In the present work, we find that JNK1 can physically interact with N-terminal transactivation domain (TAD) of TAp63. Overexpression of JNK1 inhibits TAp63γ-mediated transcription, while knockdown or inhibition of endogenous JNK1 increases transactivity of TAp63γ. Further study reveals that Ser12 site in TAD is critical for JNK1-mediated inhibition of TAp63γ. This JNK1-mediated inhibition can impair pro-apoptotic activity of TAp63γ. Together, we report a novel regulation of TAp63γ transactivity and pro-apoptotic activity mediated by JNK1. [ABSTRACT FROM AUTHOR]

Background: To identify the value of unilateral pedal lymphangiography (LPG) plus computed tomography angiography (CTA) in accurate depiction of persistent idiopathic chyluria undetectable by ordinary contrast CT.Methods: Eighteen patients 44-63 years of age with persistent idiopathic chyluria who failed conservative management were included. Ordinary CT had not revealed a chyle leak. Cystoscopy, unilateral LPG, and post-LPG CT angiography (CTA) were sequentially performed. Ligation and stripping of the perirenal lymphatics were subsequently performed guided by lymphangiography and CTA.Results: LPG and post-LPG CTA detected 17 unilateral and one bilateral chyle leaks in the 18 patients, with clear images of the communication of lymphatic vessels and the renal collecting or vascular system. The success rate was significantly better than cystoscopy (100% vs 50.0%, P = 0.005) or LPG alone (100% vs. 72.2%, P = 0.016). Chyluria resolved after surgery in all patients; no relapses were found.Conclusions: LPG plus post-LPG CTA accurately characterized perirenal lymphangiectasia that was not demonstrated by routine contrast-enhanced CT or not suitable for magnetic resonance imaging. Despite of its invasiveness, this method is a good diagnostic alternative to LPG in patients with persistent chyluria requiring surgery. [ABSTRACT FROM AUTHOR]

In urology endoscopic procedures, the Ureteral Orifice (UO) finding is crucial but may be challenging for inexperienced doctors. Generally, it is difficult to identify UOs intraoperatively due to the presence of a large median lobe, obstructing tumor, previous surgery, etc. To automatically identify various types of UOs in the video, we propose a real-time deep learning system in UO identification and localization in urinary endoscopy videos, and it can be applied to different types of urinary endoscopes. Our UO detection system is mainly based on Single Shot MultiBox Detector (SSD), which is one of the state-of-the-art deep-learning based detection networks in natural image domain. For the preprocessing, we apply both general and specific data augmentation strategies which have significantly improved all evaluation metrics. For the training steps, we only utilize rescetoscopy images which have more complex background information, and then, we use ureteroscopy images for testing. Simultaneously, we demonstrate that the model trained with rescetoscopy images can be successfully applied in the other type of urinary endoscopy images with four evaluation metrics (precision, recall, F1 and F2 scores) greater than 0.8. We further evaluate our model based on four independent video datasets which comprise both rescectoscopy videos and ureteroscopy videos. Extensive experiments on the four video datasets demonstrate that our deep-learning based UO detection system can identify and locate UOs of two different urinary endoscopes in real time with average processing time equal to 25 ms per frame and simultaneously achieve satisfactory recall and specificity.

In China, the incidence of nasopharyngeal carcinoma (NPC) and tuberculosis remains high. Additionally, there has been a marked increase in the prevalence of gout. In recent years, there has been an increase in the number of co-existing diseases. To the best of our knowledge, there have been no previous cases reported in the literature with regard to patients suffering from NPC complicated with pulmonary tuberculosis and gout. The present study describes the case of a 59-year-old male with this condition. The patient received a combination of anti-tumor, anti-tuberculosis and anti-gout therapies, and experienced no severe adverse reactions during treatment. At present, the patient's Eastern Cooperative Oncology Group performance status is good, there has been no local recurrence or distant metastasis of the NPC, and the pulmonary tuberculosis and gout are well controlled. The aim of this study was to provide insight into the treatment of patients suffering from co-existing conditions.

The combined effects of RAD and Neoral were tested in a rat orthotopic small-bowel transplantation model. Seven groups (n = 6) were involved in this study, and each one was included in three rejection models for the evaluation of host-vs.-graft disease (HVG) (LBN-F1 to LEW), graft-vs.-host disease (GVH) (LEW to LBN-F1), and combined HVG and GVH immune responses (BN to LEW). Both drugs were administered orally throughout the study. Low doses of RAD (1.0-2.5 mg/kg/day) combined with Neoral (2.0-5.0 mg/kg/day) produced strong synergistic effects in the prolongation of small-bowel graft survival in HVG (combination index, CI = 0.095, 0.1212), GVH (CI = 0.027, 0.020), and combined HVG and GVH immune responses (CI = 0.070, 0.301). The combination therapy of RAD and Neoral produces a strong synergistic effect toward the inhibition of HVG, GVH, and combined HVG and GVH immune responses in a rat small-bowel transplantation model., (Copyright 2003 Wiley-Liss, Inc.)