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Your search keyword '"Lukas Bokelmann"' showing total 5 results
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5 results on '"Lukas Bokelmann"'

1. Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP

Author

Lukas Bokelmann, Olaf Nickel, Tomislav Maricic, Svante Pääbo, Matthias Meyer, Stephan Borte, and Stephan Riesenberg

Subjects
Science
Abstract

Current SARS-CoV-2 diagnostic methods are sensitive yet poorly suited to testing whole communities on a regular basis. Here the authors present Cap-iLAMP that tests gargle lavage samples with an improved colorimetric RT-LAMP.

Published
2021
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2. A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2.

Author

Tomislav Maricic, Olaf Nickel, Ayinuer Aximu-Petri, Elena Essel, Marie Gansauge, Philipp Kanis, Dominik Macak, Julia Richter, Stephan Riesenberg, Lukas Bokelmann, Hugo Zeberg, Matthias Meyer, Stephan Borte, and Svante Pääbo

Subjects
Medicine, Science
Abstract

SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast screening method based on direct RT-qPCR to detect SARS-CoV-2 in single or pooled gargle lavages ("mouthwashes"). This method detects individuals with large viral loads (Ct≤29) and we use it to test all staff at a nursing home daily over a period of three weeks in order to reduce the risk that the infection penetrates the facility. This or similar approaches can be implemented to protect hospitals, nursing homes and other institutions in this and future viral epidemics.

Published
2020
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3. The complete mitochondrial genome of the hymenopteran hunting robber fly Dasypogon diadema

Author

Stephan Holger Drukewitz, Robin-Tobias Jauss, Lukas Bokelmann, and Björn Marcus von Reumont

Subjects
diptera, asilidae, venom, mitochondrial genome, Genetics, QH426-470
Abstract

The complete mitochondrial genome of Dasypogon diadema (Insecta: Diptera) was sequenced using the Illumina MiSeq` platform. Its mt-genome spans over 16,947 bp with a GC content of 26.6% containing 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes. The phylogenetic relationship of Dasypogon diadema and 11 other dipteran species was reconstructed and the phylogenetic position confirmed.

Published
2019
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4. A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2

Author

Svante Pääbo, Julia Richter, Tomislav Maricic, Matthias Meyer, Olaf Nickel, Dominik Macak, Stephan Borte, Stephan Riesenberg, Philipp Kanis, Lukas Bokelmann, Marie Theres Gansauge, Elena Essel, Hugo Zeberg, and Ayinuer Aximu-Petri

Subjects
0301 basic medicine, RNA viruses, Viral Diseases, Coronaviruses, Epidemiology, 0302 clinical medicine, Medical Conditions, Screening method, Medicine and Health Sciences, Mass Screening, 030212 general & internal medicine, Pathology and laboratory medicine, Virus Testing, Multidisciplinary, Transmission (medicine), Medical microbiology, Viral Load, Test (assessment), Infectious Diseases, COVID-19 Nucleic Acid Testing, Viruses, Medicine, medicine.symptom, SARS CoV 2, Pathogens, Viral load, Research Article, medicine.medical_specialty, SARS coronavirus, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Science, Real-Time Polymerase Chain Reaction, Asymptomatic, Microbiology, 03 medical and health sciences, Extraction techniques, Diagnostic Medicine, Virology, medicine, Humans, Mass screening, Biology and life sciences, business.industry, SARS-CoV-2, Organisms, Viral pathogens, COVID-19, Covid 19, RNA extraction, Microbial pathogens, Nursing Homes, Research and analysis methods, Health Care, 030104 developmental biology, Health Care Facilities, Medical Risk Factors, Emergency medicine, Health Statistics, Morbidity, Nursing homes, business, Viral Transmission and Infection
Abstract

SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast screening method based on direct RT-qPCR to detect SARS-CoV-2 in single or pooled gargle lavages (“mouthwashes”). This method detects individuals with large viral loads (Ct≤29) and we use it to test all staff at a nursing home daily over a period of three weeks in order to reduce the risk that the infection penetrates the facility. This or similar approaches can be implemented to protect hospitals, nursing homes and other institutions in this and future viral epidemics.

Published
2020

5. The complete mitochondrial genome of the hymenopteran hunting robber fly Dasypogon diadema

Author

Robin-Tobias Jauss, Stephan Holger Drukewitz, Björn M. von Reumont, and Lukas Bokelmann

Subjects
0106 biological sciences, 0301 basic medicine, Mitochondrial DNA, biology, Venom, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, 03 medical and health sciences, 030104 developmental biology, Dasypogon, Evolutionary biology, Asilidae, Genetics, Diadema, Molecular Biology, GC-content
Abstract

The complete mitochondrial genome of Dasypogon diadema (Insecta: Diptera) was sequenced using the Illumina MiSeq` platform. Its mt-genome spans over 16,947 bp with a GC content of 26.6% containing 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes. The phylogenetic relationship of Dasypogon diadema and 11 other dipteran species was reconstructed and the phylogenetic position confirmed.

Published
2019

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