1. Transformation of human diploid cells by adenovirus type 4 irradiated with ultraviolet light.
- Author
-
Hozoc M, Nastac E, Suru M, Stoian M, Bercovici S, and Cajal N
- Subjects
- Adenoviruses, Human genetics, Animals, Antigens, Viral analysis, Cells, Cultured, Cricetinae, Diploidy, Fluorescent Antibody Technique, Humans, Ultraviolet Rays, Adenoviruses, Human radiation effects, Cell Transformation, Viral radiation effects
- Abstract
Inoculation of ultraviolet (UV)-irradiated adenovirus type 4 (Ad4) led to in vitro transformation of human diploid cells (HDC). Two transformed cell lines could be established: cell line H 1418, from HDC inoculated with the 10(-3) dilution of Ad4 UV-irradiated for 20 min at a distance of 20 cm, co-cultivated with uninfected HDC, and cell line H 1557, from HDC inoculated with the 10(-2) dilution of Ad4 irradiated at the same distance for 12 min. Both transformed cell lines were resistant to superinfection with homologous virus. Virus-specific antigen could be made evident by the indirect immunofluorescence technique in the nuclei of both H 1418 and H 1557 cells.
- Published
- 1983