1. Ionic liquids based microwave-assisted extraction of lichen compounds with quantitative spectrophotodensitometry analysis
- Author
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Sophie Tomasi, Ludovic Paquin, Daniel Carrié, Joël Boustie, Sarah Bonny, Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Lichens ,MESH: Densitometry ,Liquid-Liquid Extraction ,Ionic Liquids ,MESH: Microwaves ,01 natural sciences ,Biochemistry ,MESH: Ionic Liquids ,Analytical Chemistry ,chemistry.chemical_compound ,Pertusaria ,Environmental Chemistry ,Norstictic acid ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Microwaves ,MESH: Chromatography, High Pressure Liquid ,Chromatography, High Pressure Liquid ,Spectroscopy ,Tetrahydrofuran ,Alkyl ,chemistry.chemical_classification ,Chromatography ,MESH: Liquid-Liquid Extraction ,MESH: Spectrophotometry ,biology ,010405 organic chemistry ,Depsidone ,010401 analytical chemistry ,Extraction (chemistry) ,MESH: Lichens ,biology.organism_classification ,0104 chemical sciences ,chemistry ,Spectrophotometry ,Polyphenol ,Ionic liquid ,Densitometry - Abstract
International audience; Ionic liquids based extraction method has been applied to the effective extraction of norstictic acid, a common depsidone isolated from Pertusaria pseudocorallina, a crustose lichen. Five 1-alkyl-3-methylimidazolium ionic liquids (ILs) differing in composition of alkyl chain and anion were investigated for extraction efficiency. The extraction amount of norstictic acid was determined after recovery on HPTLC with a spectrophotodensitometer. The proposed approaches (IL-MAE and IL-heat extraction (IL-HE)) have been evaluated in comparison with usual solvents such as tetrahydrofuran in heat-reflux extraction and microwave-assisted extraction (MAE). The results indicated that both the characteristics of the alkyl chain and anion influenced the extraction of polyphenolic compounds. The sulfate-based ILs [C(1)mim][MSO(4)] and [C(2)mim][ESO(4)] presented the best extraction efficiency of norstictic acid. The reduction of the extraction times between HE and MAE (2 h-5 min) and a non-negligible ratio of norstictic acid in total extract (28%) supports the suitability of the proposed method. This approach was successfully applied to obtain additional compounds from other crustose lichens (Pertusaria amara and Ochrolechia parella).
- Published
- 2011