1. Effects of regular physical activity on skeletal muscle structural, energetic, and microvascular properties in carriers of sickle cell trait
- Author
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Patrice Thiriet, Alain Francina, Hervé Sanchez, Hervé Dubouchaud, Rachel Chapot, Christian Denis, Cyril Martin, Viviane Banimbek, André Geyssant, Macias Dohbobga, Samuel Oyono-Enguéllé, Laurent Messonnier, Léonard Féasson, Lucile Vincent, Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Unité de Recherche PPEH, Service de Physiologie Clinique et de l'Exercice, Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Centre de recherche en Biologie Cellulaire (CRBM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche et d'Innovation sur le Sport (EA647) (CRIS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Unité de Pathologie Moléculaire du Globule Rouge, Hospices Civils de Lyon (HCL)-Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département des Facteurs Humains, Service de Santé des Armées-Ministère de la Défense, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire de Physiologie de l'Exercice (LPE), Université Jean Monnet - Saint-Étienne (UJM), Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF), Inter-University Laboratory of Human Movement Biology, University of Lyon, Saint-Etienne, France, parent, Laboratory of Exercise Physiology, University of Savoie, Chambery, and University Jean Monnet of Saint-Etienne, Saint-Etienne, and Laboratory of Exercise Physiology
- Subjects
Male ,Time Factors ,Physiology ,Biopsy ,[SDV]Life Sciences [q-bio] ,Hemoglobin, Sickle ,Muscle Fibers, Skeletal ,030204 cardiovascular system & hematology ,0302 clinical medicine ,Skeletal pathology ,immune system diseases ,hemic and lymphatic diseases ,ComputingMilieux_MISCELLANEOUS ,Chemistry ,Hemoglobin A ,Anatomy ,Adaptation, Physiological ,surgical procedures, operative ,medicine.anatomical_structure ,medicine.symptom ,therapeutics ,Muscle contraction ,Muscle Contraction ,Adult ,medicine.medical_specialty ,Heterozygote ,Physical activity ,Citrate (si)-Synthase ,Microcirculation ,Sickle Cell Trait ,Electron Transport Complex IV ,03 medical and health sciences ,Young Adult ,Physiology (medical) ,Internal medicine ,medicine ,Humans ,Muscle Strength ,Muscle, Skeletal ,Exercise ,Sickle cell trait ,Electron Transport Complex I ,Skeletal muscle ,Heterozygote advantage ,030229 sport sciences ,medicine.disease ,Capillaries ,Endocrinology ,Regional Blood Flow ,Exercise Test ,Physical Endurance ,Hemoglobin ,Sedentary Behavior ,Energy Metabolism ,human activities - Abstract
To assess the effects of regular physical activity on muscle functional characteristics of carriers of sickle cell trait (SCT), 39 untrained (U) and trained (T) hemoglobin (Hb)AA (CON) and SCT subjects (U-CON, n = 12; U-SCT, n = 8; T-CON, n = 10; and T-SCT, n = 9) performed a graded exercise and a time to exhaustion (Tex) test, and were subjected to a muscle biopsy. Maximal power, total work performed during Tex, citrate synthase and cytochrome c oxidase (COX) activities, respiratory chain complexes I and IV content, and capillary density (CD), diameter (COD), and surface area (CSA) were upregulated by the same proportion in T-CON and T-SCT compared with their untrained counterparts. These proportionally similar differences imply that the observed discrepancies between U-SCT and U-CON remained in the trained subjects. Specifically, both CD and COX remained and tended to remain lower, and both COD and CSA remained and tended to remain higher in T-SCT than in T-CON. Besides, carriers of SCT displayed specific adaptations with regular physical activity: creatine kinase activity; complexes II, III, and V content; and type I fiber surface area and capillary tortuosity were lower or unchanged in T-SCT than in U-SCT. In summary, our results show that 1) carriers of SCT adapted almost similarly to CON to regular physical activity for most of the studied muscle characteristics, 2) oxidative potential remains altered in physically active carriers of SCT compared with HbAA counterparts, and 3) the specific remodeling of muscle microvascular network persists in the trained state.
- Published
- 2012
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