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Biophenols (BPs) are one of the natural phytochemical products, found majorly in fruits, cereals, vegetables and beverages. BPs has gotten a lot of press in recent years due to their abundance in nature as well as biological activities. Furthermore, they are potential targets for food and pharmaceutical industries. Many extraction techniques are presented in this paper. Among all the techniques presented in this paper, pressing and solvent extraction methods are considered as traditional techniques while supercritical fluid extraction, solid phase microextraction, microwave assisted extraction, and Enzyme assisted extraction are considered as advanced techniques. The fundamentals on analysis of BPs employing different analytical instrumental techniques for qualitative and quantitative determination in environmental samples are also discussed. Only a few specific polyphenolic chemicals have had their safety and health claims formally sanctioned, therefore recent international regulatory laws are examined. In addition to the health claims and marketing of polyphenols as a functional food, the effects of food processing on polyphenol bioavailability are investigated. A number of investigational agents or polyphenol-rich content like HQC/QC and honey, from photochemistry are being also explored for recent antiviral treatment of COVID-19 and their treatment strategies. The day-by-day advancement in the analytical methodologies for BPs detection provides the platform to find out the health favorable components. This review focuses on the various extraction techniques, structure identification of plant extracts and quantification of BPs.