26 results on '"Maire, Justin"'
Search Results
2. A role for bacterial experimental evolution in coral bleaching mitigation?
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Maire, Justin and van Oppen, Madeleine J.H.
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- 2022
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3. Spatial and morphological reorganization of endosymbiosis during metamorphosis accommodates adult metabolic requirements in a weevil
- Author
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Maire, Justin, Parisot, Nicolas, Ferrarini, Mariana Galvao, Vallier, Agnès, Gillet, Benjamin, Hughes, Sandrine, Balmand, Séverine, Vincent-Monégat, Carole, Zaidman-Rémy, Anna, and Heddi, Abdelaziz
- Published
- 2020
4. Intracellular bacteria are common and taxonomically diverse in cultured and in hospite algal endosymbionts of coral reefs
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Maire, Justin, Girvan, Sam K., Barkla, Sophie E., Perez-Gonzalez, Alexis, Suggett, David J., Blackall, Linda L., and van Oppen, Madeleine J. H.
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- 2021
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5. Correction to: Intracellular bacteria are common and taxonomically diverse in cultured and in hospite algal endosymbionts of coral reefs
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Maire, Justin, Girvan, Sam K., Barkla, Sophie E., Perez-Gonzalez, Alexis, Suggett, David J., Blackall, Linda L., and van Oppen, Madeleine J. H.
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- 2021
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6. Microbiome characterization of defensive tissues in the model anemone Exaiptasia diaphana
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Maire, Justin, Blackall, Linda L., and van Oppen, Madeleine J. H.
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- 2021
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7. The transposable element-rich genome of the cereal pest Sitophilus oryzae
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Parisot, Nicolas, Vargas-Chávez, Carlos, Goubert, Clément, Baa-Puyoulet, Patrice, Balmand, Séverine, Beranger, Louis, Blanc, Caroline, Bonnamour, Aymeric, Boulesteix, Matthieu, Burlet, Nelly, Calevro, Federica, Callaerts, Patrick, Chancy, Théo, Charles, Hubert, Colella, Stefano, Da Silva Barbosa, André, Dell’Aglio, Elisa, Di Genova, Alex, Febvay, Gérard, Gabaldón, Toni, Galvão Ferrarini, Mariana, Gerber, Alexandra, Gillet, Benjamin, Hubley, Robert, Hughes, Sandrine, Jacquin-Joly, Emmanuelle, Maire, Justin, Marcet-Houben, Marina, Masson, Florent, Meslin, Camille, Montagné, Nicolas, Moya, Andrés, Ribeiro de Vasconcelos, Ana Tereza, Richard, Gautier, Rosen, Jeb, Sagot, Marie-France, Smit, Arian F. A., Storer, Jessica M., Vincent-Monegat, Carole, Vallier, Agnès, Vigneron, Aurélien, Zaidman-Rémy, Anna, Zamoum, Waël, Vieira, Cristina, Rebollo, Rita, Latorre, Amparo, and Heddi, Abdelaziz
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- 2021
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8. Weevil pgrp-lb prevents endosymbiont TCT dissemination and chronic host systemic immune activation
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Maire, Justin, Vincent-Monégat, Carole, Balmand, Séverine, Vallier, Agnès, Hervé, Mireille, Masson, Florent, Parisot, Nicolas, Vigneron, Aurélien, Anselme, Caroline, Perrin, Jackie, Orlans, Julien, Rahioui, Isabelle, Da Silva, Pedro, Fauvarque, Marie-Odile, Mengin-Lecreulx, Dominique, Zaidman-Rémy, Anna, and Heddi, Abdelaziz
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- 2019
9. Assessing the contribution of bacteria to the heat tolerance of experimentally evolved coral photosymbionts.
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Maire, Justin, Deore, Pranali, Jameson, Vanta J., Sakkas, Magdaline, Perez‐Gonzalez, Alexis, Blackall, Linda L., and van Oppen, Madeleine J. H.
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CORAL bleaching , *CLIMATE change adaptation , *CORALS , *CORAL reefs & islands , *BACTERIAL communities , *HIGH temperatures - Abstract
Coral reefs are extremely vulnerable to ocean warming, which triggers coral bleaching—the loss of endosymbiotic microalgae (Symbiodiniaceae) from coral tissues, often leading to death. To enhance coral climate resilience, the symbiont, Cladocopium proliferum was experimentally evolved for >10 years under elevated temperatures resulting in increased heat tolerance. Bacterial 16S rRNA gene metabarcoding showed the composition of intra‐ and extracellular bacterial communities of heat‐evolved strains was significantly different from that of wild‐type strains, suggesting bacteria responded to elevated temperatures, and may even play a role in C. proliferum thermal tolerance. To assess whether microbiome transplantation could enhance heat tolerance of the sensitive wild‐type C. proliferum, we transplanted bacterial communities from heat‐evolved to the wild‐type strain and subjected it to acute heat stress. Microbiome transplantation resulted in the incorporation of only 30 low‐abundance strains into the microbiome of wild‐type cultures, while the relative abundance of 14 pre‐existing strains doubled in inoculated versus uninoculated samples. Inoculation with either wild‐type or heat‐evolved bacterial communities boosted C. proliferum growth, although no difference in heat tolerance was observed between the two inoculation treatments. This study provides evidence that Symbiodiniaceae‐associated bacterial communities respond to heat selection and may contribute to coral adaptation to climate change. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Functional potential and evolutionary response to long-term heat selection of bacterial associates of coral photosymbionts.
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Maire, Justin, Philip, Gayle K., Livingston, Jadzia, Judd, Louise M., Blackall, Linda L., and van Oppen, Madeleine J. H.
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- 2023
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11. Differential responses of Ceratitis capitata to infection by the entomopathogenic fungus Purpureocillium lilacinum.
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Djobbi, Wafa, Msaad Guerfali, Meriem, Vallier, Agnès, Charaabi, Kamel, Charles, Hubert, Maire, Justin, Parisot, Nicolas, Hamden, Haytham, Fadhl, Salma, Heddi, Abdelaziz, and Cherif, Ameur
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MEDITERRANEAN fruit-fly ,ENTOMOPATHOGENIC fungi ,PHYSIOLOGY ,INTEGRATED pest control ,GENE expression profiling ,BIOLOGICAL weed control - Abstract
The medfly Ceratitis capitata is one of the most damaging fruit pests with quarantine significance due to its extremely wide host range. The use of entomopathogenic fungi constitutes a promising approach with potential applications in integrated pest management. Furthermore, developing insect control methods can involve the use of fungal machinery to cause metabolic disruption, which may increase its effectiveness by impairing insect development. Insect species, including C. capitata, relies on reproduction potential, nutrient reserves, metabolic activities, and immune response for survival. Accordingly, the purpose of this study was to investigate the impacts of the entomopathogenic fungus Purpureocillium lilacinum on C. capitata pre-mortality. The medfly V8 strain was subjected to laboratory bioassays, which consisted on determining the virulence of P. lilacinum on the medfly. Purpureocillium lilacinum was applied on abdominal topical of 5-day-old males and females. Following the fungal inoculation, we have confirmed (i) a significant increase in tissue sugar content, (ii) a significant decrease in carbohydrase activities, digestive glycosyl hydrolase, and proteinase activities in whole midguts of treated flies, (iii) the antimicrobial peptides (AMPs) genes expression profile was significantly influenced by fly gender, fly status (virgin, mature, and mated), and time after infection, but infection itself had no discernible impact on the AMPs for the genes that were examined. This study provides the first insight into how P. lilacinum could affect C. capitata physiological mechanisms and provides the foundation for considering P. lilacinum as a novel, promising biocontrol agent. [ABSTRACT FROM AUTHOR]
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- 2023
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12. Colocalization and potential interactions of Endozoicomonas and chlamydiae in microbial aggregates of the coral Pocillopora acuta.
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Maire, Justin, Tandon, Kshitij, Collingro, Astrid, van de Meene, Allison, Damjanovic, Katarina, Gotze, Cecilie Ravn, Stephenson, Sophie, Philip, Gayle K., Horn, Matthias, Cantin, Neal E., Blackall, Linda L., and van Oppen, Madeleine J. H.
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MARINE biology , *CORALS , *HORIZONTAL gene transfer , *CORAL reef conservation , *NAIVE Bayes classification , *ACETATES - Abstract
The article discusses about the colocalization and potential interactions of Endozoicomonas and chlamydiae in microbial aggregates of the coral Pocillopora acuta. It further discusses about comprehensive characterization of cell-associated microbial aggregates (CAMAs) in the coral Pocillopora acuta; detailed insight into coral endosymbionts; and also mentions about imaging techniques, laser capture microdissection and amplicon and metagenome sequencing.
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- 2023
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13. Comparing the Role of ROS and RNS in the Thermal Stress Response of Two Cnidarian Models, Exaiptasia diaphana and Galaxea fascicularis.
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Doering, Talisa, Maire, Justin, Chan, Wing Yan, Perez-Gonzalez, Alexis, Meyers, Luka, Sakamoto, Rumi, Buthgamuwa, Isini, Blackall, Linda L., and van Oppen, Madeleine J. H.
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THERMAL stresses ,CORAL bleaching ,NURSES ,NITRIC-oxide synthases ,CORAL reefs & islands ,CNIDARIA ,GREATER wax moth - Abstract
Coral reefs are threatened by climate change, because it causes increasingly frequent and severe summer heatwaves, resulting in mass coral bleaching and mortality. Coral bleaching is believed to be driven by an excess production of reactive oxygen (ROS) and nitrogen species (RNS), yet their relative roles during thermal stress remain understudied. Here, we measured ROS and RNS net production, as well as activities of key enzymes involved in ROS scavenging (superoxide dismutase and catalase) and RNS synthesis (nitric oxide synthase) and linked these metrics to physiological measurements of cnidarian holobiont health during thermal stress. We did this for both an established cnidarian model, the sea anemone Exaiptasia diaphana, and an emerging scleractinian model, the coral Galaxea fascicularis, both from the Great Barrier Reef (GBR). Increased ROS production was observed during thermal stress in both species, but it was more apparent in G. fascicularis, which also showed higher levels of physiological stress. RNS did not change in thermally stressed G. fascicularis and decreased in E. diaphana. Our findings in combination with variable ROS levels in previous studies on GBR-sourced E. diaphana suggest G. fascicularis is a more suitable model to study the cellular mechanisms of coral bleaching. [ABSTRACT FROM AUTHOR]
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- 2023
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14. Endosymbiont-containing germarium transcriptional survey in a cereal weevil depicts downregulation of immune effectors at the onset of sexual maturity.
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Galvão Ferrarini, Mariana, Vallier, Agnès, Dell’Aglio, Elisa, Balmand, Séverine, Vincent-Monégat, Carole, Debbache, Mériem, Maire, Justin, Parisot, Nicolas, Zaidman-Rémy, Anna, Heddi, Abdelaziz, and Rebollo, Rita
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CURCULIONIDAE ,YOUNG adults ,ESSENTIAL amino acids ,RICE weevil ,ANTIMICROBIAL peptides ,PYRICULARIA oryzae - Abstract
Insects often establish long-term relationships with intracellular symbiotic bacteria, i.e., endosymbionts, that provide them with essential nutrients such as amino acids and vitamins. Endosymbionts are typically confined within specialized host cells called bacteriocytes that may form an organ, the bacteriome. Compartmentalization within host cells is paramount for protecting the endosymbionts and also avoiding chronic activation of the host immune system. In the cereal weevil Sitophilus oryzae, bacteriomes are present as a single organ at the larval foregut-midgut junction, and in adults, at the apex of midgut mesenteric caeca and at the apex of the four ovarioles. While the adult midgut endosymbionts experience a drastic proliferation during early adulthood followed by complete elimination through apoptosis and autophagy, ovarian endosymbionts are maintained throughout the weevil lifetime by unknown mechanisms. Bacteria present in ovarian bacteriomes are thought to be involved in the maternal transmission of endosymbionts through infection of the female germline, but the exact mode of transmission is not fully understood. Here, we show that endosymbionts are able to colonize the germarium in oneweek-old females, pinpointing a potential infection route of oocytes. To identify potential immune regulators of ovarian endosymbionts, we have analyzed the transcriptomes of the ovarian bacteriomes through young adult development, from one-day-old adults to sexually mature ones. In contrast with midgut bacteriomes, immune effectors are downregulated in ovarian bacteriomes at the onset of sexual maturation. We hypothesize that relaxation of endosymbiont control by antimicrobial peptides might allow bacterial migration and potential oocyte infection, ensuring endosymbiont transmission. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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15. An IMD-like pathway mediates both endosymbiont control and host immunity in the cereal weevil Sitophilus spp.
- Author
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Maire, Justin, Vincent-Monégat, Carole, Masson, Florent, Zaidman-Rémy, Anna, and Heddi, Abdelaziz
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- 2018
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16. Effects of Ocean Warming on the Underexplored Members of the Coral Microbiome.
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Maire, Justin, Buerger, Patrick, Chan, Wing Yan, Deore, Pranali, Dungan, Ashley M, Nitschke, Matthew R, and van Oppen, Madeleine J H
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CORAL bleaching , *CORAL reef conservation , *MARINE heatwaves , *HEAT waves (Meteorology) , *OCEAN temperature , *CORALS , *CORAL reefs & islands - Abstract
The climate crisis is one of the most significant threats to marine ecosystems. It is leading to severe increases in sea surface temperatures and in the frequency and magnitude of marine heatwaves. These changing conditions are directly impacting coral reef ecosystems, which are among the most biodiverse ecosystems on Earth. Coral-associated symbionts are particularly affected because summer heatwaves cause coral bleaching—the loss of endosymbiotic microalgae (Symbiodiniaceae) from coral tissues, leading to coral starvation and death. Coral-associated Symbiodiniaceae and bacteria have been extensively studied in the context of climate change, especially in terms of community diversity and dynamics. However, data on other microorganisms and their response to climate change are scarce. Here, we review current knowledge on how increasing temperatures affect understudied coral-associated microorganisms such as archaea, fungi, viruses, and protists other than Symbiodiniaceae, as well as microbe-microbe interactions. We show that the coral-microbe symbiosis equilibrium is at risk under current and predicted future climate change and argue that coral reef conservation initiatives should include microbe-focused approaches. [ABSTRACT FROM AUTHOR]
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- 2022
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17. The genome of the cereal pest Sitophilus oryzae : a transposable element haven
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Parisot, Nicolas, Vargas-Chavez, Carlos, Goubert, Clément, Baa-Puyoulet, Patrice, Balmand, Séverine, Beranger, Louis, Blanc, Caroline, Bonnamour, Aymeric, Boulesteix, Matthieu, Burlet, Nelly, Calevro, Federica, Callaerts, Patrick, Chancy, Théo, Charles, Hubert, Colella, Stefano, Da Silva Barbosa, André, Dell’Aglio, Elisa, Di Genova, Alex, Febvay, Gérard, Gabaldon, Toni, Galvão Ferrarini, Mariana, Gerber, Alexandra, Gillet, Benjamin, Hubley, Robert, Hughes, Sandrine, Jacquin-Joly, Emmanuelle, Maire, Justin, Marcet-Houben, Marina, Masson, Florent, Meslin, Camille, Montagne, Nicolas, Moya, Andrés, Ribeiro de Vasconcelos, Ana Tereza, Richard, Gautier, Rosen, Jeb, Sagot, Marie-France, Smit, Arian F.A., Storer, Jessica, Vincent-Monegat, Carole, Vallier, Agnès, Vigneron, Aurélien, Zaidman-Remy, Anna, Zamoum, Waël, Vieira, Cristina, Rebollo, Rita, Latorre, Amparo, Heddi, Abdelaziz, Biologie Fonctionnelle, Insectes et Interactions (BF2I), Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre for Genomic Regulation [Barcelona] (CRG), Universitat Pompeu Fabra [Barcelona] (UPF)-Centro Nacional de Analisis Genomico [Barcelona] (CNAG), and Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
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[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,[SDV]Life Sciences [q-bio] ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,food and beverages - Abstract
Among beetles, the rice weevil Sitophilus oryzae is one of the most important pests causing extensive damage to cereal in fields and to stored grains. S. oryzae has an intracellular symbiotic relationship (endosymbiosis) with the Gram-negative bacterium Sodalis pierantonius and is a valuable model to decipher host-symbiont molecular interactions. We sequenced the Sitophilus oryzae genome using a combination of short and long reads to produce the best assembly for a Curculionidae species to date. We show that S. oryzae has undergone successive bursts of transposable element (TE) amplification, representing 72% of the genome. In addition, we show that many TE families are transcriptionally active, and changes in their expression are associated with insect endosymbiotic state. S. oryzae has undergone a high gene expansion rate, when compared to other beetles. Reconstruction of host-symbiont metabolic networks revealed that, despite its recent association with cereal weevils (30 Kyear), S. pierantonius relies on the host for several amino acids and nucleotides to survive and to produce vitamins and essential amino-acids required for insect development and cuticle biosynthesis. In addition to being an agricultural pest and a valuable endosymbiotic system, S. oryzae can be a remarkable model for studying TE evolution and regulation, along with the impact of TEs on eukaryotic genomes.
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- 2021
18. Long-Term Heat Selection of the Coral Endosymbiont Cladocopium C1 acro (Symbiodiniaceae) Stabilizes Associated Bacterial Communities.
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Buerger, Patrick, Vanstone, Ruby T., Maire, Justin, and van Oppen, Madeleine J. H.
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BACTERIAL communities ,CORALS ,HIGH temperatures ,GENETIC barcoding ,METAGENOMICS ,COMMUNITIES - Abstract
Heat-tolerant strains of the coral endosymbiont, Cladocopium C1
acro (Symbiodiniaceae), have previously been developed via experimental evolution. Here, we examine physiological responses and bacterial community composition (using 16S rRNA gene metabarcoding) in cultures of 10 heat-evolved (SS) and 9 wild-type (WT) strains, which had been exposed for 6 years to 31 °C and 27 °C, respectively. We also examine whether the associated bacterial communities were affected by a three-week reciprocal transplantation to both temperatures. The SS strains had bacterial communities with lower diversities that showed more stability and lower variability when exposed to elevated temperatures compared with the WT strains. Amplicon sequence variants (ASVs) of the bacterial genera Labrenzia, Algiphilus, Hyphobacterium and Roseitalea were significantly more associated with the SS strains compared with the WT strains. WT strains showed higher abundance of ASVs assigned to the genera Fabibacter and Tropicimonas. We hypothesize that these compositional differences in associated bacterial communities between SS and WT strains also contribute to the thermal tolerance of the microalgae. Future research should explore functional potential between bacterial communities using metagenomics to unravel specific genomic adaptations. [ABSTRACT FROM AUTHOR]- Published
- 2022
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19. Evaluating the essentiality of the primary endosymbiont of the rice weevil Sitophilus oryzae through genome analysis
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Vargas-Chavez, Carlos, Parisot, Nicolas, Goubert, C., Baa-Puyoulet, Patrice, Balmand, Severine, Boulesteix, M., Burlet, Nelly, Callaerts, Patrick, Charles, Hubert, Colella, Stefano, Febvay, Gérard, Gabaldon, Toni, Jacquin-Joly, Emmanuelle, Loska, Damian, Maire, Justin, MASSON, Florent, Meslin, Camille, montagné, N, Moya, Andres, Vincent-Monégat, Carole, Rebollo, Rita, Vallier, Agnès, Vigneron, Aurélien, Zaidman-Rémy, A., Calevro, Federica, Vieira, Cristina, Latorre, Amparo, Heddi, Abdelaziz, Charles, Hubert, Biologie Fonctionnelle, Insectes et Interactions (BF2I), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA), Eléments transposables, évolution, populations, Département génétique, interactions et évolution des génomes [LBBE] (GINSENG), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Unité de recherche Productions végétales (CRAG ANT PROD V), Institut National de la Recherche Agronomique (INRA), Barcelona Supercomputing Center - Centro Nacional de Supercomputacion (BSC - CNS), Institut d'écologie et des sciences de l'environnement de Paris (iEES), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Barcelona Institute of Science and Technology (BIST), Department ofComputationaland Systems Biology, University of Pittsburgh (PITT), Pennsylvania Commonwealth System of Higher Education (PCSHE)-Pennsylvania Commonwealth System of Higher Education (PCSHE), Institut Cavanilles de Biodiversitat i Biologia Evolutiva (ICBiBE), Universitat de València (UV), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut d'Ecologie et des Sciences de l'Environnement de Paris, Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Recherche pour le Développement (IRD [France-Ouest])-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Ecole Normale Supérieure Paris-Saclay (ENS Paris Saclay), Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
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[SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] ,fungi ,bacteria ,food and beverages ,biochemical phenomena, metabolism, and nutrition ,[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] ,Sitophilus oryzae L ,symbiosis - Abstract
International audience; The rice weevil Sitophilus oryzae is a pest insect responsible for great economic losses to the agriculture, particularly in developing countries where the damage can exceed 40% of the cereal production. This pest harbors an endosymbiont, “Candidatus Sodalis pierantonius” (cited Sodalis hereafter) that improves its fitness and invasive power. It has been demonstrated that Sodalis recently replaced the ancestral endosymbiont “Candidatus Nardonella” (cited Nardonella hereafter), which is still conserved in other members of the Dryophthoridae family. This recent acquisition raises several challenges for the host, given that the genome of Sodalis has not experienced a drastic genome size shrinkage when compared to other old-lasting insect’s endosymbionts. While the genomes of Sodalis and several strains of Nardonella are available, the insect’s genome is still needed to perform a full study of the system. To this end, we have sequenced and assembled the full genome of the weevil S. oryzae using a combination of Next Generation Sequencing data. Additionally, we have compared this genome with other available insect genomes. We found that the genome of S. oryzae encodes immune elements similar to those of other holometabolous insects, including those living without endosymbionts. Remarkably, an impressive amount of repeated elements was identified, similar to what was previously described in the Sodalis endosymbiont, suggesting the occurrence of gene rearrangement processes at this early phase of symbiogenesis. We concluded that while Sodalis is completely dependent on its host, the insect can survive without its endosymbiont.
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- 2018
20. Endosymbiosis morphological reorganization during metamorphosis diverges in weevils.
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Maire, Justin, Chouaia, Bessem, Zaidman-Rémy, Anna, and Heddi, Abdelaziz
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CURCULIONIDAE , *ENDOSYMBIOSIS , *FLUORESCENCE in situ hybridization , *METAMORPHOSIS , *RICE weevil - Abstract
Virtually all animals associate with beneficial symbiotic bacteria. Whether and how these associations are modulated across a host's lifecycle is an important question in disentangling animal-bacteria interactions. We recently reported a case of complete morphological reorganization of symbiosis during metamorphosis of the cereal weevil, Sitophilus oryzae. In this model, the bacteriome, a specialized organ that houses the intracellular bacterium Sodalis pierantonius, undergoes a two-phase remodeling program synchronously driven by host and endosymbiont, resulting in a localization shift and the formation of multiple new bacteriomes. Here, we provide comparative data in a closely-related coleopteran, the red palm weevil Rhynchophorus ferrugineus, which is associated with the ancestral endosymbiont Nardonella. Using cell imaging experiments, we show that the red pal weevil bacteriome remains unchanged during metamorphosis, hence contrasting with what we reported in the cereal weevil S. oryzae. These findings highlight the complexity and divergence of host-symbiont interactions and their intertwining with host development, even in closely-related species. Abbreviations: DAPI: 4′,6-diamidino-2-phenylindole; FISH: Fluorescence in situ hybridization; T3SS: Type III secretion system. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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21. Effects of symbiotic status on cellular immunity dynamics in Sitophilus oryzae.
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López-Madrigal, Sergio, Maire, Justin, Balmand, Séverine, Zaidman-Rémy, Anna, and Heddi, Abdelaziz
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SYMBIOSIS , *CELLULAR immunity , *RICE weevil , *BLOOD cells , *PROTEOBACTERIA - Abstract
Many insects maintain intracellular symbiosis with mutualistic bacteria that improve their adaptive capabilities in nutritionally poor habitats. Adaptation of insect immune systems to such associations has been shown in several symbiotic consortia, including that of the rice weevil Sitophilus oryzae with the gammaproteobacterium Sodalis pierantonius . Although authors have mostly focused on the role of humoral immunity in host-symbiont interactions, recent studies suggest that symbiotic bacteria may also interfere with the cellular, hemocyte-based, immunity. Here, we have explored hemocyte dynamics in S. oryzae in the presence or absence of S. pierantonius, and in response to bacterial challenges. We have identified five morphotypes within larval hemocytes, whose abundance and morphometry drastically change along insect development. We show that hemocytes make part of the weevil immune system by responding to pathogenic infections. In contrast with previous results on other insect species, however, our analyses did not reveal any symbiotic-dependent modulation of the hemocyte global population. [ABSTRACT FROM AUTHOR]
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- 2017
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22. Intracellular Bacterial Symbionts in Corals: Challenges and Future Directions.
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Maire, Justin, Blackall, Linda L., and van Oppen, Madeleine J. H.
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SYMBIODINIUM ,CORALS ,CORAL reefs & islands ,CORAL bleaching ,SYMBIOSIS ,EUKARYOTES ,ARCHAEBACTERIA - Abstract
Corals are the main primary producers of coral reefs and build the three-dimensional reef structure that provides habitat to more than 25% of all marine eukaryotes. They harbor a complex consortium of microorganisms, including bacteria, archaea, fungi, viruses, and protists, which they rely on for their survival. The symbiosis between corals and bacteria is poorly studied, and their symbiotic relationships with intracellular bacteria are only just beginning to be acknowledged. In this review, we emphasize the importance of characterizing intracellular bacteria associated with corals and explore how successful approaches used to study such microorganisms in other systems could be adapted for research on corals. We propose a framework for the description, identification, and functional characterization of coral-associated intracellular bacterial symbionts. Finally, we highlight the possible value of intracellular bacteria in microbiome manipulation and mitigating coral bleaching. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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23. Chlamydiae in corals: shared functional potential despite broad taxonomic diversity.
- Author
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Maire J, Collingro A, Horn M, and van Oppen MJH
- Abstract
Cnidarians, such as corals and sea anemones, associate with a wide range of bacteria that have essential functions, including nutrient cycling and the production of antimicrobial compounds. Within cnidarians, bacteria can colonize all microhabitats including the tissues. Among them are obligate intracellular bacteria of the phylum Chlamydiota (chlamydiae) whose impact on cnidarian hosts and holobionts, especially corals, remain unknown. Here, we conducted a meta-analysis of previously published 16S rRNA gene metabarcoding data from cnidarians (e.g. coral, jellyfish, and anemones), eight metagenome-assembled genomes (MAGs) of coral-associated chlamydiae, and one MAG of jellyfish-associated chlamydiae to decipher their diversity and functional potential. While the metabarcoding dataset showed an enormous diversity of cnidarian-associated chlamydiae, six out of nine MAGs were affiliated with the Simkaniaceae family. The other three MAGs were assigned to the Parasimkaniaceae, Rhabdochlamydiaceae, and Anoxychlamydiaceae, respectively. All MAGs lacked the genes necessary for an independent existence, lacking any nucleotide or vitamin and most amino acid biosynthesis pathways. Hallmark chlamydial genes, such as a type III secretion system, nucleotide transporters, and genes for host interaction, were encoded in all MAGs. Together these observations suggest an obligate intracellular lifestyle of coral-associated chlamydiae. No unique genes were found in coral-associated chlamydiae, suggesting a lack of host specificity. Additional studies are needed to understand how chlamydiae interact with their coral host, and other microbes in coral holobionts. This first study of the diversity and functional potential of coral-associated chlamydiae improves our understanding of both the coral microbiome and the chlamydial lifestyle and host range., Competing Interests: The authors declare they have no competing interests., (© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.)
- Published
- 2024
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24. Chlamydiae as symbionts of photosynthetic dinoflagellates.
- Author
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Maire J, Collingro A, Tandon K, Jameson VJ, Judd LM, Horn M, Blackall LL, and van Oppen MJH
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- Animals, Chlamydiales genetics, Chlamydiales classification, Chlamydiales physiology, Chlamydiales isolation & purification, Genome, Bacterial, Anthozoa microbiology, Metagenome, In Situ Hybridization, Fluorescence, Dinoflagellida microbiology, Dinoflagellida genetics, Dinoflagellida physiology, Symbiosis, Phylogeny, Photosynthesis
- Abstract
Chlamydiae are ubiquitous intracellular bacteria and infect a wide diversity of eukaryotes, including mammals. However, chlamydiae have never been reported to infect photosynthetic organisms. Here, we describe a novel chlamydial genus and species, Candidatus Algichlamydia australiensis, capable of infecting the photosynthetic dinoflagellate Cladocopium sp. (originally isolated from a scleractinian coral). Algichlamydia australiensis was confirmed to be intracellular by fluorescence in situ hybridization and confocal laser scanning microscopy and temporally stable at the population level by monitoring its relative abundance across four weeks of host growth. Using a combination of short- and long-read sequencing, we recovered a high-quality (completeness 91.73% and contamination 0.27%) metagenome-assembled genome of A. australiensis. Phylogenetic analyses show that this chlamydial taxon represents a new genus and species within the Simkaniaceae family. Algichlamydia australiensis possesses all the hallmark genes for chlamydiae-host interactions, including a complete type III secretion system. In addition, a type IV secretion system is encoded on a plasmid and has previously been observed for only three other chlamydial species. Twenty orthologous groups of genes are unique to A. australiensis, one of which is structurally similar to a protein known from Cyanobacteria and Archaeplastida involved in thylakoid biogenesis and maintenance, hinting at potential chlamydiae interactions with the chloroplasts of Cladocopium cells. Our study shows that chlamydiae infect dinoflagellate symbionts of cnidarians, the first photosynthetic organism reported to harbor chlamydiae, thereby expanding the breadth of chlamydial hosts and providing a new contribution to the discussion around the role of chlamydiae in the establishment of the primary plastid., (© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.)
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- 2024
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25. Tissue-associated and vertically transmitted bacterial symbiont in the coral Pocillopora acuta.
- Author
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Maire J, Tsang Min Ching SJ, Damjanovic K, Epstein HE, Judd LM, Blackall LL, and van Oppen MJH
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- Animals, Phylogeny, RNA, Ribosomal, 16S genetics, Bacteria genetics, Metagenome, Coral Reefs, Symbiosis, Anthozoa microbiology, Gammaproteobacteria genetics
- Abstract
Coral microhabitats are colonized by a myriad of microorganisms, including diverse bacteria which are essential for host functioning and survival. However, the location, transmission, and functions of individual bacterial species living inside the coral tissues remain poorly studied. Here, we show that a previously undescribed bacterial symbiont of the coral Pocillopora acuta forms cell-associated microbial aggregates (CAMAs) within the mesenterial filaments. CAMAs were found in both adults and larval offspring, suggesting vertical transmission. In situ laser capture microdissection of CAMAs followed by 16S rRNA gene amplicon sequencing and shotgun metagenomics produced a near complete metagenome-assembled genome. We subsequently cultured the CAMA bacteria from Pocillopora acuta colonies, and sequenced and assembled their genomes. Phylogenetic analyses showed that the CAMA bacteria belong to an undescribed Endozoicomonadaceae genus and species, which we propose to name Candidatus Sororendozoicomonas aggregata gen. nov sp. nov. Metabolic pathway reconstruction from its genome sequence suggests this species can synthesize most amino acids, several B vitamins, and antioxidants, and participate in carbon cycling and prey digestion, which may be beneficial to its coral hosts. This study provides detailed insights into a new member of the widespread Endozoicomonadaceae family, thereby improving our understanding of coral holobiont functioning. Vertically transmitted, tissue-associated bacteria, such as Sororendozoicomonas aggregata may be key candidates for the development of microbiome manipulation approaches with long-term positive effects on the coral host., (© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.)
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- 2024
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26. Endosymbiont-containing germarium transcriptional survey in a cereal weevil depicts downregulation of immune effectors at the onset of sexual maturity.
- Author
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Ferrarini MG, Vallier A, Dell'Aglio E, Balmand S, Vincent-Monégat C, Debbache M, Maire J, Parisot N, Zaidman-Rémy A, Heddi A, and Rebollo R
- Abstract
Insects often establish long-term relationships with intracellular symbiotic bacteria, i.e., endosymbionts, that provide them with essential nutrients such as amino acids and vitamins. Endosymbionts are typically confined within specialized host cells called bacteriocytes that may form an organ, the bacteriome. Compartmentalization within host cells is paramount for protecting the endosymbionts and also avoiding chronic activation of the host immune system. In the cereal weevil Sitophilus oryzae, bacteriomes are present as a single organ at the larval foregut-midgut junction, and in adults, at the apex of midgut mesenteric caeca and at the apex of the four ovarioles. While the adult midgut endosymbionts experience a drastic proliferation during early adulthood followed by complete elimination through apoptosis and autophagy, ovarian endosymbionts are maintained throughout the weevil lifetime by unknown mechanisms. Bacteria present in ovarian bacteriomes are thought to be involved in the maternal transmission of endosymbionts through infection of the female germline, but the exact mode of transmission is not fully understood. Here, we show that endosymbionts are able to colonize the germarium in one-week-old females, pinpointing a potential infection route of oocytes. To identify potential immune regulators of ovarian endosymbionts, we have analyzed the transcriptomes of the ovarian bacteriomes through young adult development, from one-day-old adults to sexually mature ones. In contrast with midgut bacteriomes, immune effectors are downregulated in ovarian bacteriomes at the onset of sexual maturation. We hypothesize that relaxation of endosymbiont control by antimicrobial peptides might allow bacterial migration and potential oocyte infection, ensuring endosymbiont transmission., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Ferrarini, Vallier, Dell’Aglio, Balmand, Vincent-Monégat, Debbache, Maire, Parisot, Zaidman-Rémy, Heddi and Rebollo.)
- Published
- 2023
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