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266 results on '"Matsubayashi, Makoto"'

8. Redescription, molecular characterisation and Wolbachia endosymbionts of Mansonella (Tupainema) dunni (Mullin & Orihel, 1972) (Spirurida: Onchocercidae) from the common treeshrew Tupaia glis Diard & Duvaucel (Mammalia: Scandentia) in Peninsular Malaysia

Author

Mat Udin, Ahmad Syihan, Uni, Shigehiko, Rodrigues, Jules, Martin, Coralie, Junker, Kerstin, Agatsuma, Takeshi, Low, Van Lun, Saijuntha, Weerachai, Omar, Hasmahzaiti, Zainuri, Nur Afiqah, Fukuda, Masako, Matsubayashi, Makoto, Kimura, Daisuke, Takaoka, Hiroyuki, and Ramli, Rosli

Published
2024
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12. Evaluation of the detection method by a flotation method using a wire loop for gastrointestinal parasites.

Author

Takano, Aruto, Morinaga, Daikichi, Teramoto, Isao, Hatabu, Toshimitsu, Kido, Yasutoshi, Kaneko, Akira, Hatta, Takeshi, Tsuji, Naotoshi, Uni, Shigehiko, Sasai, Kazumi, Katoh, Hiromitsu, and Matsubayashi, Makoto

Subjects
FLOTATION, OOCYSTS, CHICKENS, CENTRIFUGATION, EGGS
Abstract

Infections by gastrointestinal parasites are found in a variety of animals worldwide. For the diagnosis of such infections, the flotation method is commonly used to detect parasitic microorganisms, such as oocysts or eggs, in feces. Instead of adding a flotation solution after the final centrifugation step and using a cover slip to collect the parasites, the method using a wire loop for the recovery of the organisms has been reported as one of alternative methods. However, the recovery rates of microorganisms from the flotation method have not been analysed. In the present study, the utility of a flotation method with the use of a wire loop of 8 mm in diameter (the loop method) was evaluated using different numbers of E. tenella oocysts and Heterakis gallinarum eggs, and chicken fecal samples collected at the farms. Consequently, we found that the oocysts and eggs in tubes could be collected at a ratio of 2.00 to 3.08. Thus, our results indicate that the loop method is a simple and time saving method, implicating the application for the estimated OPG/ EPG (Oocysts/Eggs per gram) of the samples. [ABSTRACT FROM AUTHOR]

Published
2024
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14. Description and molecular characterisation of Pelecitus copsychi Uni, Mat Udin & Martin n. sp. (Nematoda: Onchocercidae) from the white-rumped shama Copsychus malabaricus (Scopoli) (Passeriformes: Muscicapidae) of Pahang, Malaysia

Author

Uni, Shigehiko, Mat Udin, Ahmad Syihan, Tan, Poai Ean, Rodrigues, Jules, Martin, Coralie, Junker, Kerstin, Agatsuma, Takeshi, Low, Van Lun, Lim, Yvonne Ai-Lian, Saijuntha, Weerachai, Omar, Hasmahzaiti, Zainuri, Nur Afiquah, Fukuda, Masako, Kimura, Daisuke, Matsubayashi, Makoto, Uga, Shoji, Takaoka, Hiroyuki, Azirun, Mohd Sofian, and Ramli, Rosli

Published
2022
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26. Improved molecular identification of Strongyloides myopotami in nutrias using fecal samples.

Author

MORI, Yuga, NAKA, Atsushi, KODA, Ryosuke, ISHIZUKA, Yuzuru, HINENOYA, Atsushi, SHIBAHARA, Tomoyuki, SASAI, Kazumi, and MATSUBAYASHI, Makoto

Subjects
INTESTINAL parasites, HELMINTHS, FECES, DNA, RIBOSOMAL RNA
Abstract

Strongyloides myopotami is an intestinal nematode parasite of nutrias. Identification of S. myopotami is conducted based on the morphological characteristics of adult worms or cultured larvae. To widely and effectively understand the infection in nutrias, it would be preferable to develop the molecular identification using a few grams of the feces. Here, we attempted to identify S. myopotami using DNA extracted from eggs obtained from fecal samples. Among previously reported primer pairs targeting the 18S rRNA gene of Strongyloides spp., most could not be successful. We newly designed primers that successfully amplified the partial sequences in S. myopotami, resulting in being sequenced. Our simple protocol can be useful in nationwide surveys for clarifying the risk of human infection. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Description, molecular characteristics and Wolbachia endosymbionts of Onchocerca borneensis Uni, Mat Udin & Takaoka n. sp. (Nematoda: Filarioidea) from the Bornean bearded pig Sus barbatus Müller (Cetartiodactyla: Suidae) of Sarawak, Malaysia

Author

Uni, Shigehiko, Mat Udin, Ahmad Syihan, Agatsuma, Takeshi, Junker, Kerstin, Saijuntha, Weerachai, Bunchom, Naruemon, Fukuda, Masako, Martin, Coralie, Lefoulon, Emilie, Labat, Amandine, Khan, Faisal Ali Anwarali, Low, Van Lun, Cheah, Phaik Leng, Lim, Yvonne Ai-Lian, Ramli, Rosli, Belabut, Daicus Martin, Zainuri, Nur Afiqah, Matsubayashi, Makoto, Omar, Hasmahzaiti, Bhassu, Subha, Uga, Shoji, Hashim, Rosli, Takaoka, Hiroyuki, and Azirun, Mohd Sofian

Published
2020
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39. Phylogenetic characterization of Isospora jaracimrmani oocysts from a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) reared at a zoo in Ishikawa, Japan

Author

EKAWASTI, Fitrine, KITAGAWA, Kazuya, DOMAE, Hiroshi, WARDHANA, April Hari, NAGASAWA, Junki, SHIBAHARA, Tomoyuki, TOKORO, Masaharu, SASAI, Kazumi, and MATSUBAYASHI, Makoto

Subjects
Feces, Isospora, Japan, parasitic diseases, Oocysts, Animals, Wildlife Science, Lizards, Note, oocyst, veiled chameleon, Phylogeny
Abstract

Oocysts of Isospora sp. were detected in the feces of a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) kept at a zoo in Ishikawa, Japan. Phylogenetic analysis placed the sequence in the cluster of Isospora spp. isolated from reptiles. Based on a comparison of morphological data of ten previously reported Isospora species from the Chamaeleonidae family, this isolate was morphologically similar to I. jaracimrmani, which has been considered to be a virulent species. This case study suggests the possibility that species of Isospora might not always cause disease because the animal that shed these oocysts showed no symptoms for more than two months.

Published
2021

40. RNA activation in ticks.

Author

Kwofie, Kofi Dadzie, Hernandez, Emmanuel Pacia, Anisuzzaman, Kawada, Hayato, Koike, Yuki, Sasaki, Sana, Inoue, Takahiro, Jimbo, Kei, Mikami, Fusako, Ladzekpo, Danielle, Umemiya-Shirafuji, Rika, Yamaji, Kayoko, Tanaka, Tetsuya, Matsubayashi, Makoto, Alim, Md Abdul, Dadzie, Samuel Kweku, Iwanaga, Shiroh, Tsuji, Naotoshi, and Hatta, Takeshi

Subjects
TICKS, GENETIC overexpression, GENETIC regulation, DOUBLE-stranded RNA, ARGONAUTE proteins, EGGS
Abstract

RNA activation (RNAa) is a burgeoning area of research in which double-stranded RNAs (dsRNAs) or small activating RNAs mediate the upregulation of specific genes by targeting the promoter sequence and/or AU-rich elements in the 3′- untranslated region (3'-UTR) of mRNA molecules. So far, studies on the phenomenon have been limited to mammals, plants, bacteria, Caenorhabditis elegans, and recently, Aedes aegypti. However, it is yet to be applied in other arthropods, including ticks, despite the ubiquitous presence of argonaute 2 protein, which is an indispensable requirement for the formation of RNA-induced transcriptional activation complex to enable a dsRNA-mediated gene activation. In this study, we demonstrated for the first time the possible presence of RNAa phenomenon in the tick vector, Haemaphysalis longicornis (Asian longhorned tick). We targeted the 3ʹ-UTR of a novel endochitinase-like gene (HlemCHT) identified previously in H. longicornis eggs for dsRNA-mediated gene activation. Our results showed an increased gene expression in eggs of H. longicornis endochitinase-dsRNA-injected (dsHlemCHT) ticks on day-13 post-oviposition. Furthermore, we observed that eggs of dsHlemCHT ticks exhibited relatively early egg development and hatching, suggesting a dsRNA-mediated activation of the HlemCHT gene in the eggs. This is the first attempt to provide evidence of RNAa in ticks. Although further studies are required to elucidate the detailed mechanism by which RNAa occurs in ticks, the outcome of this study provides new opportunities for the use of RNAa as a gene overexpression tool in future studies on tick biology, to reduce the global burden of ticks and tick-borne diseases. [ABSTRACT FROM AUTHOR]

Published
2023
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46. Swine multifocal ulcerative colitis and crypt abscesses associated with Entamoeba polecki subtype 3 and Salmonella Typhimurium

Author

ITO, Hirotaka, HOSOKAWA, Kumiko, KAWAMURA, Midori, ITO, Naomi, ABETO, Yusuke, MATSUBAYASHI, Makoto, SASAI, Kazumi, and SHIBAHARA, Tomoyuki

Subjects
Diarrhea, Salmonella typhimurium, Swine Diseases, Salmonella Infections, Animal, Entamoebiasis, Coinfection, Swine, animal diseases, Salmonella enterica serovar Typhimurium, multifocal ulcerative colitis, bacterial infections and mycoses, Note, post-weaning pig, digestive system diseases, Abscess, Entamoeba, fluids and secretions, Entamoeba polecki subtype 3, Japan, Pathology, Animals, Colitis, Ulcerative
Abstract

Piglets aged approximately 50 days exhibited diarrhea and wasting. Multiple white foci were detected in the colon of a dead piglet; histopathological findings revealed multifocal ulcers and crypt abscesses with Entamoeba trophozoites and gram-negative bacilli in the piglet. These pathogens were identified as Entamoeba polecki subtype 3 and Salmonella enterica serovar Typhimurium, respectively. Numerous E. polecki subtype 3 trophozoites were located on the edge of the ulcerative and necrotic lesions in the lamina propria. Crypt abscesses were associated with S. Typhimurium. These results suggest that E. polecki subtype 3 caused multifocal ulcerative colitis accompanied by crypt abscesses with S. Typhimurium in the piglet. This study is the first report of colitis with E. polecki subtype 3 and S. Typhimurium coinfection.

Published
2020

49. Longistatin in tick saliva blocks advanced glycation end-product receptor activation

Author

Hatta, Takeshi, Miyoshi, Takeharu, Matsubayashi, Makoto, Islam, M. Khyrul, Alim, M. Abdul, Anas, M. Abu, Hasan, M. Mehedi, Matsumoto, Yasunobu, Yamamoto, Yasuhiko, Yamamoto, Hiroshi, Fujisaki, Kozo, and Tsuji, Naotoshi

Subjects
Advanced glycation end products -- Properties, Salivary glands -- secretions, Immune response -- Observations, Ticks -- Physiological aspects, Host-parasite relationships -- Physiological aspects, Saliva -- Properties, Health care industry
Abstract

Ticks are notorious hematophagous ectoparasites and vectors of many deadly pathogens. As an effective vector, ticks must break the strong barrier provided by the skin of their host during feeding, and their saliva contains a complex mixture of bioactive molecules that paralyze host defenses. The receptor for advanced glycation end products (RAGE) mediates immune cell activation at inflammatory sites and is constitutively and highly expressed in skin. Here, we demonstrate that longistatin secreted with saliva of the tick Haemaphysalislongicornis binds RAGE and modulates the host immune response. Similar to other RAGE ligands, longistatin specifically bound the RAGE V domain, and stimulated cultured HUVECs adhered to a longistatin-coated surface; this binding was dramatically inhibited by soluble RAGE or RAGE siRNA. Treatment of HUVECs with longistatin prior to stimulation substantially attenuated cellular oxidative stress and prevented NF-κB translocation, thereby reducing adhesion molecule and cytokine production. Recombinant longistatin inhibited RAGE-mediated migration of mouse peritoneal resident cells (mPRCs) and ameliorated inflammation in mouse footpad edema and pneumonia models. Importantly, tick bite upregulated RAGE ligands in skin, and endogenous longistatin attenuated RAGE-mediated inflammation during tick feeding. Our results suggest that longistatin is a RAGE antagonist that suppresses tick bite-associated inflammation, allowing successful blood-meal acquisition from hosts., Introduction Ticks are notorious blood-sucking ectoparasites, and about 1,000 tick species are distributed throughout the world. They have immense vector potential and serve as a unique vector of various deadly [...]

Published
2014
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