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10. Development and Validation of Markers for the Fertility Restorer Gene Rf1 in Sunflower

Author

Horn, Renate, Radanovic, Aleksandra, Fuhrmann, Lena, Sprycha, Yves, Hamrit, Sonia, Jockovic, Milan, Miladinovic, Dragana, and Jansen, Constantin

Subjects
Genetic Markers, P<%2Fu>olymerase+chain+reaction+A<%2Fu>mplification+of+M<%2Fu>ultiple+S<%2Fu>pecific+A<%2Fu>lleles+%28PAMSA%29%22">Polymerase chain reaction Amplification of Multiple Specific Alleles (PAMSA), Genetic Linkage, Genes, Plant, Polymorphism, Single Nucleotide, Article, Chromosomes, Plant, lcsh:Chemistry, sunflower (Helianthus annuus), fertility restoration, lcsh:QH301-705.5, amplicon targeted sequencing, Sequence Characterized Amplified Region (SCAR), Polymerase chain reaction Amplification of Multiple Specific Alleles (PAMSA), Fertility, pentatricopeptide repeat, lcsh:Biology (General), lcsh:QD1-999, breeding, Helianthus, marker assisted selection, candidate genes, Genome, Plant, restorer gene Rf1
Abstract

Hybrid breeding in sunflowers based on CMS PET1 requires development of restorer lines carrying, in most cases, the restorer gene Rf1. Markers for marker-assisted selection have been developed, but there is still need for closer, more versatile, and co-dominant markers linked to Rf1. Homology searches against the reference sunflower genome using sequences of cloned markers, as well as Bacterial Artificial Chromosome (BAC)-end sequences of clones hybridizing to them, allowed the identification of two genomic regions of 30 and 3.9 Mb, respectively, as possible physical locations of the restorer gene Rf1 on linkage group 13. Nine potential candidate genes, encoding six pentatricopeptide repeat proteins, one tetratricopeptide-like helical domain, a probable aldehyde dehydrogenase 22A1, and a probable poly(A) polymerase 3 (PAPS3), were identified in these two genomic regions. Amplicon targeted next generation sequencing of these nine candidate genes for Rf1 was performed in an association panel consisting of 27 maintainer and 32 restorer lines and revealed the presence of 210 Single Nucleotide Polymorphisms (SNPs) and 67 Insertions/Deletions (INDELs). Association studies showed significant associations of 10 SNPs with fertility restoration (p-value &lt, 10&minus, 4), narrowing Rf1 down to three candidate genes. Three new markers, one co-dominant marker 67N04_P and two dominant markers, PPR621.5R for restorer, and PPR621.5M for maintainer lines were developed and verified in the association panel of 59 sunflower lines. The versatility of the three newly developed markers, as well as of three existing markers for the restorer gene Rf1 (HRG01 and HRG02, Cleaved Amplified Polymorphic Sequence (CAPS)-marker H13), was analyzed in a large association panel consisting of 557 accessions.

Published
2019

12. Pathogenic comparison of highly virulent O. cumana affecting sunflower in Moldova, the South of Russian Federation, Serbia and Spain

Author

García-Carneros, Ana B., Dedic, Bosko, Miladinovic, Dragana, Molinero-Ruiz, Leire, Ministry of Education, Science and Technological Development (Serbia), and Ministerio de Educación y Ciencia (España)

Subjects
Europe, Virulence, Pathogenic characterization, Broomrape, Helianthus annuus L, Pathogenic race
Abstract

Trabajo presentado en el Third Internacional Symposium on broomrape (Orobanche spp.) in Sunflower, celebrado en Córdoba (España) del 3 al 6 de junio de 2014., The most effective method for controlling the parasitic weed Orobanche cumana (sunflower broomrape) is the incorporation of genetic resistance into cultivated sunflower. According to different genes of resistance that are overcome, several races of the parasite are pathogenically identified. Race G is the most virulent one because it infects the universal resistant inbred line P96. Besides, populations of O. cumana are molecularly clustered according to geographical origin, and pathogenic traits are secondary criteria for genetic differentiation. In this work highly virulent parasite accessions collected in Moldova, the South of Russian Federation, Serbia and Spain were pathogenically compared. Six accessions were inoculated onto differentials of highly virulent races of the parasite: NR5, L86 and P96. Plants were grown under shadehouse and glasshouse for ten weeks following a factorial on a completely randomised design. At the end of the experiment none of the accessions infected P96. According to the reactions of the inbred lines NR5 and L86, accessions from Moldova and Serbia were identified as race E, and accessions from Spain were identified as race F. Accessions from the Russian Federation showed intermediate pathogenic behaviour onto NR5 and L86. Since a highly virulent biotype of O. cumana is present in Rostov oblast and seed used in this work comes from individual broomrape plants, the presence of race F in this area might be confirmed through the study of genetically heterogeneous parasite accessions from the area. Our results show that, in order to efficiently manage the genetic resistance into cultivated sunflower, there is a need for a worldwide study of highly virulent populations of O. cumana., Financial support from the Spanish Ministry of Education and Science (research project AGL2010-17909). Broomrape collection in Serbia is a part of project TR31025 supported by Ministry of Education, Science and Technological Development of Republic of Serbia.

Published
2014

14. Identification and validation of breeder-friendly DNA markers for Pl gene in sunflower.

Author

Imerovski, Ivana, Dimitrijevic, Aleksandra, Miladinovic, Dragana, Jocic, Sinisa, Dedic, Bosko, Cvejic, Sandra, and Surlan-Momirovic, Gordana

Subjects
PLANT breeding, BIOMARKERS, PLANT DNA, SUNFLOWER genetics, DOWNY mildew diseases, FUNGAL diseases of plants, CROP yields, SUNFLOWERS
Abstract

Downy mildew is a fungal disease of sunflower that can lead to severe yield losses. The damage caused by the pathogen can be controlled by growing resistant sunflower varieties. Gene Pl was introgressed into cultivated sunflower from the wild species Helianthus argophyllus and provides resistance against all known downy mildew races. In this study, we used a mapping population from the cross-RHA 419/RHA-N-49. We identified a new co-segregating simple sequence repeat marker ORS675 and confirmed the co-segregation of markers ORS716 and ORS662 with Pl gene. The markers were validated on two registered resistant inbred lines RHA 443 and RHA 464, as well as on twenty inbred lines RH 1-20 obtained through methods of classical breeding. Molecular marker ORS716 was assessed for usefulness in selecting resistant progeny in 12 BC populations. Markers were found to be valuable for molecular breeding in diverse genetic backgrounds and enabled transfer of the resistance gene in different sunflower genotypes. [ABSTRACT FROM AUTHOR]

Published
2014
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15. Identification of PCR markers linked to different Or genes in sunflower.

Author

Imerovski, Ivana, Dimitrijevic, Aleksandra, Miladinovic, Dragana, Dedic, Bosko, Jocic, Sinisa, Kovacevic, Branislav, Obreht, Dragana, and Friedt, W.

Subjects
POLYMERASE chain reaction, SUNFLOWERS, PARASITIC plants, PLANT breeding, BROOMRAPES, COMMON sunflower, PLANT genetics, GENETIC polymorphisms in plants
Abstract

Broomrape is a parasitic plant that significantly decreases yield of sunflower. Breeding for resistance has proved to be the most efficient method for suppressing broomrape infestation in the field; however, new races of parasite constantly emerge, and new resistance genes need to be discovered and introduced into cultivated sunflower lines. The aim of this work was to test SSR markers from linkage group 3 ( LG3) to investigate whether they could be used for identification of a particular Or gene. Twenty sunflower inbred lines were used, and polymorphism between the lines with various resistance genes and genetic background was investigated. The used markers revealed DNA polymorphism between the investigated lines. Strong association of markers from LG3 with Or 6, as well as Or 4 and Or 2 genes, was found. Identified markers could be used for introduction of these resistance genes into commercial sunflower lines and for establishment and identification of differential lines. [ABSTRACT FROM AUTHOR]

Published
2013
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16. Sclerotinia Wilt Occurrence on Sunflower in Vojvodina, Serbia.

Author

Tančić, Sonja, Dedić, Boško, Jocić, Siniša, Balalić, Igor, Lacok, Nada, Miladinovic, Dragana, and Miklic, Vladimir

Subjects
SCLEROTINIA, PLANT species, PLANT growth, SUNFLOWERS, PLANT diseases
Abstract

Copyright of Field & Vegetable Crops Research / Ratarstvo i povrtarstvo is the property of Institute of Field & Vegetable Crops and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2011
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17. Contributors

Author

Aguirrezábal, L.A.N., Alberio, C., Balalic, Igor, Block, Charles C., Bootello, Miguel A., Dijkstra, Albert J., Feoli, Carlos E., Fernández, Juan, Fernández-Martínez, José M., Garcés, Rafael, González-Pérez, Sergio, Gulya, Thomas J., Hanzel, James J., Harveson, Robert M., Ingaramo, Jorge, Inturrisi, Lucky, Izquierdo, N.G., Jocić, Siniša, Kaya, Yalcin, Kemper, Timothy, Kleingartner, Larry, Clef, Etienne Le, León, Alberto, Linz, George M., Markell, Samuel G., Miladinović, Dragana, Milic, Vladimir, Pedroche, Justo, Pérez-Vich, Begoña, Prasifka, Jarrad R., Ruiz-Méndez, M. Victoria, Troncoso-Ponce, Manuel A., Velasco, Leonardo, Venegas-Calerón, Monica, and Zambelli, Andrés

Published
2015
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18. [Untitled]

Author

Ricroch, Agnès, Eriksson, Dennis, Miladinović, Dragana, Sweet, Jeremy, Van Laere, Katrijn, and Woźniak-Gientka, Ewa

Abstract

This open access book is an update of genome editing techniques applied to a range of plants. We discuss the latest techniques and applications to cereals, roots and tubers, oilseed crops, fruit and forest trees, vegetables, legumes and algae including resistance to biotic and abiotic stresses, improved quality, drug production, yield and adaptation to climate change. The regulations in different countries worldwide, the patentibility and the perception by society of the applications of new genomic techniques are examined. This book is written by a multidisciplinary and multisectoral collective of high-profile scientists and other experts belonging to the COST Action network PlantEd, which is mainly European but with contributions from American, Australian, Canadian, Chinese, Indian, Iranian, Pakistani and Peruvian scientists. The book is aimed at a wide audience consisting of students, academics, private and public breeders, other actors in the food and bioeconomy value chains and policy and law makers. This is an open access book.

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19. [Untitled]

Author

Ricroch, Agnès, Eriksson, Dennis, Miladinović, Dragana, Sweet, Jeremy, Van Laere, Katrijn, and Woźniak-Gientka, Ewa

Abstract

This open access book is an update of genome editing techniques applied to a range of plants. We discuss the latest techniques and applications to cereals, roots and tubers, oilseed crops, fruit and forest trees, vegetables, legumes and algae including resistance to biotic and abiotic stresses, improved quality, drug production, yield and adaptation to climate change. The regulations in different countries worldwide, the patentibility and the perception by society of the applications of new genomic techniques are examined. This book is written by a multidisciplinary and multisectoral collective of high-profile scientists and other experts belonging to the COST Action network PlantEd, which is mainly European but with contributions from American, Australian, Canadian, Chinese, Indian, Iranian, Pakistani and Peruvian scientists. The book is aimed at a wide audience consisting of students, academics, private and public breeders, other actors in the food and bioeconomy valuechains and policy and law makers. This is an open access book.

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