7 results on '"Milhim, Bruno H. G. A."'
Search Results
2. Influence of previous Zika virus infection on acute dengue episode.
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Estofolete, Cassia F., Versiani, Alice F., Dourado, Fernanda S., Milhim, Bruno H. G. A., Pacca, Carolina C., Silva, Gislaine C. D., Zini, Nathalia, Santos, Barbara F. dos, Gandolfi, Flora A., Mistrão, Natalia F. B., Garcia, Pedro H. C., Rocha, Rodrigo S., Gehrke, Lee, Bosch, Irene, Marques, Rafael E., Teixeira, Mauro M., da Fonseca, Flavio G., Vasilakis, Nikos, and Nogueira, Maurício L.
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DENGUE hemorrhagic fever ,ZIKA virus infections ,DENGUE ,ZIKA virus - Abstract
Background: The co-circulation of flaviviruses in tropical regions has led to the hypothesis that immunity generated by a previous dengue infection could promote severe disease outcomes in subsequent infections by heterologous serotypes. This study investigated the influence of antibodies generated by previous Zika infection on the clinical outcomes of dengue infection. Methodology/Principal findings: We enrolled 1,043 laboratory confirmed dengue patients and investigated their prior infection to Zika or dengue. Severe forms of dengue disease were more frequent in patients with previous Zika infection, but not in those previously exposed to dengue. Conclusions/Significance: Our findings suggest that previous Zika infection may represent a risk factor for subsequent severe dengue disease, but we did not find evidence of antibody-dependent enhancement (higher viral titer or pro-inflammatory cytokine overexpression) contributing to exacerbation of the subsequent dengue infection. Author summary: Although dengue is a disease known for years in the world and has been affecting several continents, some aspects remain unclear. One of them is about the possible factors that may influence the development of severe forms of the disease. Much has been discussed about the influence of a previous dengue episode, but the global spreading of other flaviviruses to areas where dengue was already circulating has aroused interest regarding a role like the other dengue serotypes. In this sense, the recent spreading of the Zika virus has become a factor of interest. In this study, the prior Zika virus infection was associated with a higher frequency of more severe forms in subsequent dengue. Preliminary findings did not suggest that the mechanism is the same one triggered in secondary dengue, known as antibody-dependent enhancement. These findings are a stimulus to develop further research that can understand the potential mechanisms involved in the pathogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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3. Performance of VIDAS ® Diagnostic Tests for the Automated Detection of Dengue Virus NS1 Antigen and of Anti-Dengue Virus IgM and IgG Antibodies: A Multicentre, International Study.
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Versiani, Alice F., Kaboré, Antoinette, Brossault, Ludovic, Dromenq, Loïc, dos Santos, Thayza M. I. L., Milhim, Bruno H. G. A., Estofolete, Cássia F., Cissé, Assana, Sorgho, Pegdwendé Abel, Senot, Florence, Tessonneau, Marie, Diagbouga, Serge, and Nogueira, Mauricio L.
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DENGUE hemorrhagic fever ,DENGUE viruses ,IMMUNOGLOBULIN G ,DIAGNOSIS methods ,IMMUNOGLOBULINS ,ANTIGENS - Abstract
Dengue is a serious mosquito-transmitted disease caused by the dengue virus (DENV). Rapid and reliable diagnosis of DENV infection is urgently needed in dengue-endemic regions. We describe here the performance evaluation of the CE-marked VIDAS
® dengue immunoassays developed for the automated detection of DENV NS1 antigen and anti-DENV IgM and IgG antibodies. A multicenter concordance study was conducted in 1296 patients from dengue-endemic regions in Asia, Latin America, and Africa. VIDAS® dengue results were compared to those of competitor enzyme-linked immunosorbent assays (ELISA). The VIDAS® dengue assays showed high precision (CV ≤ 10.7%) and limited cross-reactivity (≤15.4%) with other infections. VIDAS® DENGUE NS1 Ag showed high positive and negative percent agreement (92.8% PPA and 91.7% NPA) in acute patients within 0–5 days of symptom onset. VIDAS® Anti-DENGUE IgM and IgG showed a moderate-to-high concordance with ELISA (74.8% to 90.6%) in post-acute and recovery patients. PPA was further improved in combined VIDAS® NS1/IgM (96.4% in 0–5 days acute patients) and IgM/IgG (91.9% in post-acute patients) tests. Altogether, the VIDAS® dengue NS1, IgM, and IgG assays performed well, either alone or in combination, and should be suitable for the accurate diagnosis of DENV infection in dengue-endemic regions. [ABSTRACT FROM AUTHOR]- Published
- 2023
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4. Arboviral Infections in Neurological Disorders in Hospitalized Patients in São José do Rio Preto, São Paulo, Brazil.
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Milhim, Bruno H. G. A., da Rocha, Leonardo C., Terzian, Ana C. B., Mazaro, Carolina C. P., Augusto, Marcos T., Luchs, Adriana, Zini, Nathalia, Sacchetto, Livia, dos Santos, Barbara F., Garcia, Pedro H. C., Rocha, Rodrigo S., Liso, Elisabete, Brienze, Vânia M. S., da Silva, Gislaine C. D., Vasilakis, Nikos, Estofolete, Cássia F., and Nogueira, Maurício L.
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NEUROLOGICAL disorders , *PERIPHERAL nervous system , *HOSPITAL patients , *HEALTH planning , *MEDICAL case management , *MENINGITIS , *ARBOVIRUS diseases - Abstract
Arbovirus infections are increasingly important causes of disease, whose spectrum of neurological manifestations are not fully known. This study sought to retrospectively assess the incidence of arboviruses in cerebrospinal fluid samples of patients with neurological symptoms to inform diagnosis of central and peripheral nervous system disorders. A total of 255 cerebrospinal fluid (CSF) samples collected from January 2016 to December 2017 were tested for dengue virus (DENV 1-4), Zika virus (ZIKV), and Chikungunya virus (CHIKV) in addition to other neurotropic arboviruses of interest, using genetic and serologic assays. Of the 255 CSF samples analyzed, 3.53% (09/255) were positive for arboviruses presenting mainly as meningitis, encephalitis, and cerebrovascular events, of which ZIKV was detected in 2.74% (7/255), DENV in 0.78% (2/255), in addition to an identified ILHV infection that was described previously. All the cases were detected in adults aged 18 to 74 years old. Our findings highlight the scientific and clinical importance of neurological syndromes associated with arboviruses and demonstrate the relevance of specific laboratory methods to achieve accurate diagnoses as well as highlight the true dimension of these diseases to ultimately improve public health planning and medical case management. [ABSTRACT FROM AUTHOR]
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- 2022
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5. Fatal Outcome of Ilheus Virus in the Cerebrospinal Fluid of a Patient Diagnosed with Encephalitis.
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Milhim, Bruno H. G. A., Estofolete, Cássia F., Rocha, Leonardo C. da, Liso, Elisabete, Brienze, Vânia M. S., Vasilakis, Nikos, Terzian, Ana C. B., and Nogueira, Maurício L.
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CEREBROSPINAL fluid , *CENTRAL nervous system , *ENCEPHALITIS , *CEREBROSPINAL fluid examination , *SYMPTOMS , *VIRUSES - Abstract
Ilheus virus is an arbovirus with the potential for central nervous system involvement. Accurate diagnosis is a challenge due to similar clinical symptoms and serologic cross-reactivity with other flaviviruses. Here, we describe the first documented case of a fatal outcome following the identification of Ilheus virus in the cerebrospinal fluid (CSF) of a patient with cerebral encephalitis in Brazil. [ABSTRACT FROM AUTHOR]
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- 2020
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6. Flavivirus Infection Associated with Cerebrovascular Events.
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Estofolete, Cássia F., Milhim, Bruno H. G. A., Zini, Nathalia, Scamardi, Samuel N., Selvante, Joana D'Arc, Vasilakis, Nikos, and Nogueira, Maurício L.
- Abstract
Arthropod-borne viruses (arboviruses) of the genus Flavivirus are distributed globally and cause significant human disease and mortality annually. Flavivirus infections present a spectrum of clinical manifestations, ranging from asymptomatic to severe manifestations, including hemorrhage, encephalitis and death. Herein, we describe 3 case reports of cerebrovascular involvement in patients infected by dengue and Zika viruses in Sao Jose do Rio Preto, São Paulo State, Brazil, a hyperendemic area for arbovirus circulation, including dengue, yellow fever, chikungunya and Saint Louis encephalitis viruses. Our findings highlight the potential threat that unusual clinical manifestations may pose to arbovirus disease management and recovery. [ABSTRACT FROM AUTHOR]
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- 2020
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7. Diabetes as risk factor to severity of dengue in naïve patients.
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Dos Santos BF, Gandolfi FA, Milhim BHGA, Dourado FS, Silva GCD, Zini N, Gratão VHR, Mariani MP, Abbas TN, Garcia PHC, Rocha RS, Vasilakis N, Nogueira ML, and Estofolete CF
- Abstract
Background: Dengue cases can progress to severe ant life-threating forms particularly in subsequent heterologous infections. However, recent studies had explored additional risk factors, including underlying health conditions, even in individuals without prior exposure to dengue, notably, in patients with endothelial dysfunction and chronic inflammation. This study examines the link between diabetes and the development of severe dengue disease in dengue-naive patients during the 2019 dengue outbreak in São Jose do Rio Preto, Brazil., Methodology: We enrolled 529 laboratory-confirmed dengue cases, identified through DENV RT-PCR or NS1 antigen assays in a hospital cohort of acute febrile illness. Subsequently, we investigated the presence of anti-dengue and anti-Zika IgG antibodies. Samples testing positive for Zika were excluded from the analyses. Two groups were analyzed: naïve (DV-), and dengue history (DV+)., Results: Initially, presence of diabetes and kidney disease, as well as being dengue-naive, were associated with a higher frequency of severe and potentially severe clinical outcomes. Multivariate analysis identified diabetes as a risk factor, while the presence of anti-dengue antibodies was considered protective. Analysis of dengue naïve samples, highlighted diabetes as an independent risk factor to severe forms of dengue disease. In DV+ patients, no condition was highlighted as a risk factor by univariate analysis or multivariate analysis., Conclusions: We investigated and confirmed diabetes as a risk factor for severe dengue disease in individuals without prior dengue or Zika exposure. Our conclusions raise significant concerns given diabetes' ever increasing global prevalence and its potential impact on patients with or previous dengue exposure., Competing Interests: Competing interests The authors declare no competing interests.
- Published
- 2024
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