1. Modifying Graft-versus-Host Disease in a Humanized Mouse Model by Targeting Macrophages or B-Cells.
- Author
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Hogenes, Marieke C. H., van Dorp, Suzanne, van Kuik, Joyce, Monteiro, Filipa R. P., ter Hoeve, Natalie, Guedes, Liane, van Dijk, Marijke R., Martens, Anton C., and de Weger, Roel A.
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GRAFT versus host disease ,B cells ,MACROPHAGES ,MOUSE diseases ,THERAPEUTICS - Abstract
Humanized mouse models can well be modified to study specific aspects of Graft-versus-Host Disease (GvHD). This paper shows the results of both macrophage depletion and (early) B-cell depletion in a humanized mouse model using RAG2-/-γc-/- mice injected with HuPBMCs. Macrophage depletion showed a significant decrease in survival and also lead to a change in the histomorphology of the xenogeneic reaction. Higher levels of infiltrating B-cells were observed in various organs of mice depleted for macrophages. With (early) B-cell depletion using Rituximab, a clear improvement on clinical symptoms was observed, even when probably only inactivated B-cells were deleted. However, the histological examinations only showed a significant morphological effect on liver fibrosis. This may be related to a difference in the mRNA levels of TGF-β. Also, lower mRNA levels of Tregs in some organs were observed after Rituximab treatment, which contradicts that a higher number of Tregs would always be related to less severe GvHD. Our data show that both macrophage depletion and (early) B-cell depletion in a xenogeneic mouse model can influence the clinical, histological, and cytokine production of a GvHD response. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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