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Objective: to analyze the results of blood sera comprehensive study of the local population of the RR, conducted in 2021 to assess the state of natural and zoonotic focus of CHF, WNF, ITBB, tularemia and risk of possible spread of Haemorrhagic Fever with Renal Syndrome (HFRS). Materials and methods: 424 blood serums obtained in 2021 from healthy donors living in 10 administrative territories of the Russian Federation were tested by enzyme immunoassay. Results: in 2021 the detection rates of specific antibodies in the studied areas were: WNF — 9,9 %, HFRS — 4,5 %, ITBB — 2,6 %, CHF — 0,7 %, tularemia — 22,6 %. When comparing the results obtained in 2020 and 2021, the difference in the proportions of seropositive samples is not statistically significant. Conclusions: the study of the immune layer to NFI in selected population groups confirms the circulation of pathogens in RR territory. The monitoring results demonstrate the extensive, mostly combined nosoareals of NFI of viral and bacterial etiology and the contact of the local population with them.

Objective: to ground the choice of ships’ ballast water decontamination methods with disinfection substance under the title «Biopag-D».Materials and Methods: for study of methods of ballast water decontamination in preliminary experiment in vitro with selected concentration «Biopag-D», in which disinfectant has a the strong antibacterial effect against the test microorganisms.Results: based on the established concentrations the methods of ballast water decontamination are developed (irrigation of empty ballast tanks, adding the working concentration of the disinfectant in complete ballast tanks and the combined method).Conclusion: the offered methods of ship’s ballast water decontamination with disinfection substance are a new disinfection technology and will assist contribute to the implementation of the international quality standard of ballast water in the Russian Federation.