Search

We propose a framework of Lagrangian Coherent Structures (LCSs) to enable passive open-loop control of tonal sound generated during thermoacoustic instability. Experiments were performed in a laboratory-scale bluff-body stabilized turbulent combustor in the state of thermoacoustic instability. We use dynamic mode decomposition on the flow-field to identify dynamical regions where the acoustic frequency is dominant. We find that the separating shear layer from the backward-facing step of the combustor envelops a cylindrical vortex in the outer recirculation zone, which eventually impinges on the top wall of the combustor during thermoacoustic instability. We track the saddle points in this shear layer emerging from the backward-facing step over several acoustic cycles. A passive control strategy is then developed by injecting a steady stream of secondary air targeting the identified optimal location where the saddle points spend a majority of their time in a statistical sense. After implementing the control action, the resultant flow-field is also analyzed using LCS to understand the key differences in flow dynamics. We find that the shear layer emerging from the dump plane is deflected in a direction almost parallel to the axis of the combustor after the control action. This deflection, in turn, prevents the shear layer from enveloping the vortex and impinging on the combustor walls, resulting in a drastic reduction in the amplitude of the sound produced. [ABSTRACT FROM AUTHOR]

Turbulent kinetic energy (TKE) is a quantity of primary importance in shock–turbulence interaction (STI). Linear interaction analysis (LIA) serves as a theoretical method to predict the amplification of TKE in STI. LIA is constrained by low-amplitude fluctuations and neglects nonlinear effects. In this paper, we explore the nonlinear amplification of the kinetic energy of fluctuations in the interaction between a vorticity wave and a shock wave that serves as a building block flow for STI. A weakly nonlinear framework (WNLF) is introduced to analyze nonlinear effects in fluctuation kinetic energy (FKE) and identify the dominant physical mechanisms driving its amplification. The theoretical framework is validated through high-accuracy numerical simulations of shock–vorticity wave interactions. The simulation results are compared with the predictions derived from the WNLF for a range of intensities and inclinations of shock-upstream vorticity fluctuations at different Mach numbers, and WNLF is found to successfully scale the numerical data for FKE, thus confirming the validity and applicability of the framework. According to WNLF findings, at lower supersonic Mach numbers, the intermodal interaction between vorticity–vorticity modes is important, whereas the interaction between vorticity and acoustic modes becomes dominant at higher Mach numbers. Using the intermodal interactions, a model based on WNLF is proposed to predict TKE amplification in STI. In comparison to direct numerical simulation, the WNLF based model predicts the TKE amplification for moderate turbulent Mach number and lower supersonic flow Mach numbers. This is a significant improvement over the LIA results available in the literature. [ABSTRACT FROM AUTHOR]

Interactions of shear fluctuations with shock waves are ubiquitous in high-speed flow applications from scramjet propulsion to cosmic events like supernova explosions. They also serve as fundamental building blocks for the study of shock–turbulence interaction. In this work, we study the nonlinear effects in pressure arising due to the interaction of a normal shock with a two-dimensional shear wave. It employs the weakly nonlinear framework (WNLF) developed recently for vorticity amplification by Thakare et al. ["A weakly nonlinear framework to study shock–vorticity interaction," J. Fluid Mech. 933, A48 (2022)]. The analysis includes the effect of intermodal interactions that is neglected in the widely used linear interaction analysis (LIA) of shock–turbulence interaction. It is found that the deformation of the shock wave and the fluctuation mass flux normal to the shock contribute to the dominant physical mechanisms responsible for the observed nonlinearities. Interestingly, the WNLF predicts a drop in mean pressure behind the shock due to a second-order intermodal interaction, which is consistent with the well-established results by Lele ["Shock-jump relations in a turbulent flow," Phys. Fluids A 4, 2900–2905 (1992)] at low Mach numbers and brings out additional effects of shock deformation that are important at higher Mach numbers. We extend the WNLF to three-dimensional interaction of homogeneous isotropic turbulence with a normal shock. Comparison with existing direct numerical simulation data shows good agreement for low turbulent Mach numbers, which is a significant improvement over the prediction capability of LIA. We also compute the dilatation fields from WNLF and use them to distinguish between the acoustic and non-acoustic components of the second-order pressure fluctuations generated by the shock wave. [ABSTRACT FROM AUTHOR]

Introduction: The Coronavirus Disease 2019 (COVID-19) pandemic has imposed an unprecedented burden on our healthcare system. Serological testing for Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) antibodies serves as an useful marker for determining an infection by the virus in the recent past and the immune response. The immune response, including the humoral response to the infection is one of them and the knowledge in this area is still evolving. Virus specific antibodies are expected to help in eliminating the virus and to provide protective immunity against reinfection. Aim: To serially monitor the total antibody response to SARSCoV-2 in order to gain better insight into the duration of antibody persistence. Materials and Methods: This prospective observational study was conducted in 66 Healthcare Workers (HCW) with a history of Reverse Transcription-Polymerase Chain Reaction (RTPCR) proven COVID-19 infection. The study was conducted between May 2020 to April 2021 at the Suburban diagnostics Central Processing Laboratory, Mumbai, Maharashtra, India. Serum samples were serially examined for the presence of total antibodies against the Nucleocapsid (N) protein of SARS-CoV-2 upto 180 days postinfection. A further follow-up examination was done at 360 days. A qualitative Electrochemiluminescence Immunoassay (ECLIA) assay was used for assessment of the antibody response. The chi-square or Fisher-exact test was used to compare categorical variables and the Mann-Whitney U test, Kruskal Wallis test and student t-test were used to compare continuous variables across groups. For assessing relationship between variables, the Pearson test or linear regression were used as appropriate. Results: Out of 66 healthcare workers, 32 were male (48.5%) and 34 were females (51.5%) with the median age of 29.5 years. Out of 66 cases, 62 (94%) cases developed antibodies against SARS-CoV-2 at different time intervals, 48 cases during the 14-30 day interval, 10 cases during the 31-60 day interval, three cases during the 61-90 day interval and one case during the 90-120 days interval. Thirty one out of 35 (88%) cases that could be followed-up at 360 days showed persistence of antibodies. No patient reported symptoms which would warrant a repeat RT-PCR test. Conclusion: This study showed that the antibody response to SARS-CoV-2 virus was sustained for 12 months postinfection in most cases. The absence of fresh infection in these cases during the study period suggests that the antibodies might protect against reinfection with the virus. So, it may be safe to defer vaccination in postinfection cases by 6-9 months thereby saving precious resources. [ABSTRACT FROM AUTHOR]

Linear interaction analysis (LIA) is routinely used to study the shock-turbulence interaction in supersonic and hypersonic flows. It is based on the inviscid interaction of elementary Kovásznay modes with a shock discontinuity. LIA neglects nonlinear effects, and hence it is limited to small-amplitude disturbances. In this work, we extend the LIA framework to study the fundamental interaction of a two-dimensional vorticity wave with a normal shock. The predictions from a weakly nonlinear framework are compared with high-order accurate numerical simulations over a range of wave amplitudes (∊), incidence angles (α) and shock-upstream Mach numbers (M¹). It is found that the nonlinear generation of vorticity at the shock has a significant contribution from the intermodal interaction between vorticity and acoustic waves. Vorticity generation is also strongly influenced by the curvature of the normal shock wave, especially for high incidence angles. Further, the weakly nonlinear analysis is able to predict the correct scaling of the nonlinear effects observed in the numerical simulations. The analysis also predicts a Mach number dependent limit for the validity of LIA in terms of the maximum possible amplitude of the upstream vorticity wave. [ABSTRACT FROM AUTHOR]

The mechanism underpinning the generation of screech tones has remained an open question for many years. In this paper, direct experimental observations of the shock-leakage mechanism first proposed by Manning & Lele (AIAA Paper 1998, p. 282) are presented. Ultra-high-speed schlieren images are filtered to preserve only upstream-propagating components, with the upstream motion of the shock tip and subsequent emission of an acoustic wave visible for a number of operating conditions. The flow visualizations are supported by the ray-tracing model for shock leakage of Shariff & Manning (Phys. Fluids., vol. 25, issue 7, 2013, 076103), applied to velocity fields corresponding to a reconstructed screech cycle. The predictions of the model, when applied to real data, are in close agreement with the phenomena observed in the flow visualizations. It is demonstrated that shock leakage does not necessarily occur either at the point of maximum wave amplitude or maximum vorticity fluctuation. While the first point of shock leakage is shown to vary between cases, sound emission from multiple sources is observed for most cases considered. Finally, it is shown that variations in vortex strength captured in the velocity data are sufficient to explain variation in shock-leakage location observed in the flow visualization data. [ABSTRACT FROM AUTHOR]

This paper reports the first experimental observations of various novel unsteady flame propagation modes in a two-dimensional, high-aspect ratio rectangular quartz channel with a positive wall temperature gradient for rich premixed propane–air mixtures. Various flame propagation modes are observed on progressively increasing the mixture velocity, while keeping the equivalence ratio fixed: FREI (Flame with Repetitive Extinction and Ignition) mode, oscillating FREI mode, oscillating flame mode, and wavy flame mode. The FREI mode resembles the classical FREI flame propagation reported earlier in the literature. In the oscillating FREI mode, the flame front oscillates in the transverse direction between the upper and lower walls of the channel, while propagating upstream as in the classical FREI mode. A sudden peak in flame intensity is observed in this mode before its extinction at an upstream location. In the oscillating flame propagation mode, the flame front anchors itself at an axial location and exhibits periodic oscillations in the transverse direction without extinction. In the wavy flame mode the flame anchoring happens at a location close to the downstream end of the channel. The flame front exhibits visibly irregular fluctuations, while anchored at this axial location. A Fast Fourier transform analysis of the flame intensity data shows that FREI and oscillating FREI modes consist of a single dominant frequency of ∼100 Hz, whereas multiple dominant frequencies are present for oscillating and wavy flame modes. The appearance of these multiple oscillating and propagating flame modes is attributed to flame bifurcation behavior due to thermal-wall coupling in the channel. [ABSTRACT FROM AUTHOR]

Experiments were performed in a partially premixed bluff-body stabilized combustor in the regimes of combustion noise, intermittency, and thermoacoustic instability. Simultaneous measurements of unsteady pressure fluctuations and flow-field using time-resolved two-component particle image velocimetry reveal dominant dynamics at 141.9 Hz which is responsible for thermoacoustic instability. In the intermittent regime that presages thermoacoustic instability, there are two distinct frequencies: a low-frequency component at 30.7 Hz dominant in the velocity spectra (hydrodynamic mode) and a higher frequency component at 176.4 Hz dominant in the pressure spectra (acoustic mode). Examining the phase relationship between the two modes in the intermittent regime using a variant of the Dynamic Mode Decomposition (DMD) confirms that the appearance of bursts of periodic pressure oscillations coincide with the time instants when the hydrodynamic and the acoustic modes are phase synchronized. To identify the flow structure dynamics observed only during sound production, we compute ridges in the fields of backward-time finite time Lyapunov exponents. The roll up of shear layers from the dump plane and the leading edge of the bluff body and subsequent impingement on combustor walls are identified as the dominant features of the flow during thermoacoustic instability as well as during the bursting stage of intermittency. We show convincingly that these identified dynamics correspond to the acoustic mode using DMD filtered flow fields comprising only of the acoustic mode. [ABSTRACT FROM AUTHOR]

Recently, there has been a growing interest in understanding and characterising intermittent burst oscillations that presage the onset of combustion instability. We construct a deterministic model to capture this intermittency route to instability in a bluff-body stabilised combustor by coupling the equations governing vortex shedding and the acoustic wave propagation in a confinement. A feedback mechanism is developed wherein the sound generated due to unsteady combustion affects the vortex shedding. This feedback leads to a variation in the time of impingement of the vortices with the bluff body causing the system to exhibit chaos, intermittency, and limit cycle oscillations. Experimental validation of the model is provided using various precursor measures that quantify the observed intermittent states. [ABSTRACT FROM PUBLISHER]

In this study, we attempt to provide a repertoire of measures to forewarn the onset of impending flow-induced mechanical oscillations via online health monitoring. To illustrate the principles, the flow of air through a pipe terminated by a circular orifice plate is investigated at various flow velocities using a suitably placed pressure transducer. It is observed that the regimes corresponding to the production of a tone is presaged by operating conditions that display temporarily intermittent bursts of periodic pressure oscillations that emerge from a background of loweramplitude aperiodic fluctuations. The various model-free measures prescribed in this paper serve as efficient precursors by characterizing these intermittent states, which can potentially arise in aeroacoustic systems when the flow is highly unsteady or turbulent. [ABSTRACT FROM AUTHOR]

Background: Casein-free, gluten-free diets have been reported to mitigate some of the inflammatory gastrointestinal and behavioral traits associated with autism, but the mechanism for this palliative effect has not been elucidated. We recently showed that the opioid peptide beta-casomorphin-7, derived from bovine (bBCM7) milk, decreases cysteine uptake, lowers levels of the antioxidant glutathione (GSH) and decreases the methyl donor S-adenosylmethionine (SAM) in both Caco-2 human GI epithelial cells and SH-SY5Y human neuroblastoma cells. While human breast milk can also release a similar peptide (hBCM-7), the bBCM7 and hBCM-7 vary greatly in potency; as the bBCM-7 is highly potent and similar to morphine in it's effects. Since SAM is required for DNA methylation, we wanted to further investigate the epigenetic effects of these food-derived opioid peptides. In the current study the main objective was to characterize functional pathways and key genes responding to DNA methylation effects of food-derived opioid peptides. Methods: SH-SY5Y neuroblastoma cells were treated with 1 µM hBCM7 and bBCM7 and RNA and DNA were isolated after 4 h with or without treatment. Transcriptional changes were assessed using a microarray approach and CpG methylation status was analyzed at 450,000 CpG sites. Functional implications from both endpoints were evaluated via Ingenuity Pathway Analysis 4.0 and KEGG pathway analysis was performed to identify biological interactions between transcripts that were significantly altered at DNA methylation or transcriptional levels (p < 0.05, FDR <0.1). Results: Here we show that hBCM7 and bBCM7, as well as morphine, cause epigenetic changes affecting gene pathways related to gastrointestinal disease and inflammation. These epigenetic consequences exhibited the same potency order as opiate inhibition of cysteine uptake insofar as hBCM7 was less potent than bBCM7, which was less potent than morphine. Conclusion: Our findings indicate that epigenetic effects of milk-derived opiate peptides may contribute to GI dysfunction and inflammation in sensitive individuals. While the current study was performed using SH-SY5Y neuronal cellular models, similar actions on other cells types might combine to cause symptoms of intolerance. These actions may provide a potential contributing mechanism for the beneficial effects of a casein-free diet in alleviating gastrointestinal symptoms in neurological conditions including autism and other conditions. Lastly, our study also contributes to the evolving awareness of a "gut-brain connection". [ABSTRACT FROM AUTHOR]

The dynamic transitions preceding lean blowout were investigated experimentally in a laboratory scale turbulent combustor by systematically varying the flow Reynolds number. Previous studies on combustor dynamics have shown that the onset of large-amplitude, combustion-driven oscillations is, at times, presaged by intermittent bursts of high-amplitude periodic pressure pulsations. These intermittent bursts appear in a near random fashion amidst regions of aperiodic low-amplitude fluctuations, provided the underlying flow-field is turbulent. In the present study, we show that intermittent burst oscillations are also observed in combustors close to the lean blowout limit. We show that such intermittent oscillations emerge through the establishment of homoclinic orbits in the phase space of pressure oscillations. The formation of such orbits points to the complex nature of the interaction between the hydrodynamics and acoustic subsystems, which operate over a range of different time scales. High-speed flame images reveal that the intermittent states observed prior to lean blowout correspond to aperiodic detachment of the flame from the bluff-body lip. These findings are consistent with other reports of possibly intermittent states in the literature. [ABSTRACT FROM AUTHOR]

In combustors, the transition from low-amplitude, aperiodic fluctuations termed combustion noise to large-amplitude, periodic oscillations termed combustion instability is presaged by an intermediate regime in flow conditions characterized by bursts of intermittent, high-amplitude, periodic oscillations that appear in a near-random fashion amidst aperiodic fluctuations. In this study, we show that, a reduced-order model from first principles that incorporates the hydrodynamic–acoustic coupling can capture these intermittent burst oscillations and the subsequent flow-acoustic lock-in observed in combustors. The physical mechanism that leads to intermittency in pressure fluctuations is described using the model. The paper concludes by illustrating through the model, ideas that use intermittency in the signal as an early warning signal—a precursor—to an impending combustion instability. [ABSTRACT FROM AUTHOR]

Germ Cell Tumours (GCTs) in children are uncommon, constituting approximately only 3% of all malignancies in children younger than 15 years of age. Primary extragonadal GCTs constitute only 1-5% of all GCTs and a retroperitoneal site is seen only in 4% of all extragonadal GCTs. Extragonadal GCTs arise from local transformation of primordial germ cells which have been misplaced during the migration of these cells through the midline dorsal mesentery in the fourth-sixth week of embryogenesis. GCTs in children show remarkable variability in age, site, presentation and histology. This is the case of a three-year-old male child who presented with a history of an abdominal swelling which was rapidly progressive in nature. Radiology showed a large retroperitoneal mass and lesions in the liver. Histopathology, immunohistochemistry and serum Alpha-fetoprotein (AFP) values confirmed a diagnosis of pure primary extragonadal yolk sac tumour. [ABSTRACT FROM AUTHOR]

Combustion noise has been traditionally thought of as stochastic fluctuations present in the background of the dynamics in combustors amongst the flow, heat release and the chamber acoustics. Through a series of determinism tests, we show that these aperiodic fluctuations are in fact chaotic of moderately high dimensions (d0 ≈ 8-10). These chaotic fluctuations then transition to high amplitude combustion instability when the operating conditions are varied towards leaner equivalence ratios. Precursors to such a transition from chaos to dynamics dominated by periodic oscillations are of interest to designers and operators of combustors in estimating the boundaries of operability. We introduce a test for chaos, known as 0-1 test for chaos in the literature, as a measure of the proximity of the combustor to an impending instability. The measure is robust and shows a smooth transition for variation in flow conditions towards instability enabling thresholds to be set for operational boundaries. [ABSTRACT FROM AUTHOR]

In this paper, we show how the phenomenon of intermittency observed in systems with turbulent flow-sound interaction is related to the formation of homoclinic orbits in the phase space. Such orbits that emerge via the intersection of the stable and unstable manifold of an equilibrium configuration result from interactions that happen at multiple spatial/temporal scales associated with turbulent convection and wave propagation. Through a quantification of the time spent by the dynamics in the aperiodic states using recurrence plots, we show how the presence of homoclinic orbits in the dynamics may be convincingly demonstrated, which is often not possible through a visual inspection of the phase space of the attractor. [ABSTRACT FROM AUTHOR]

Experiments are performed in a partially premixed bluff body-stabilized turbulent combustor by varying the mean flow velocity. Simultaneous measurements obtained for unsteady pressure, velocity, and heat release rate are used to investigate the dynamic regimes of intermittency (10.1 m/s) and thermoacoustic instability (12.3 m/s). Using wavelet analysis, we show that during intermittency, modulation of heat release rate occurring at the acoustic frequency fa by the heat release rate occurring at the hydrodynamic frequency fh results in epochs of heat release rate fluctuations where the heat release rate is phase locked with the acoustic pressure. We also show that the flame position during intermittency and thermoacoustic instability are essentially dictated by saddle point dynamics in the dump plane and the leading edge of the bluff body. [ABSTRACT FROM AUTHOR]

To evaluate the safety and toxicity profile of a chitosan (CS) and poly(lactic-co-glycolic) acid (PLGA)-based sustained release methotrexate (MTX) intravitreal micro-implant in normal rabbit eyes using non-invasive testing that included electroretinography (ERG), ultrasound biomicroscopy (US), slit-lamp biomicroscopy (SLB), funduscopy, and intraocular pressure (IOP). PLGA-coated CS-based micro-implants containing 400 μg of MTX and placebo (without drug) micro-implants were surgically-implanted in the vitreous of the right and the left eyes, respectively, in each of the thirty New Zealand rabbits. ERG, US, SLB, funduscopy, and IOP were assessed in both eyes at pre-determined time points (days: 1, 3, 7, 14, 28 and 56). The safety of micro-implants was assessed by analyzing the ERG data using different statistical models, to quantify and compare the functional integrity of the retina. Further, US, funduscopy, SLB and IOP determined the condition of the retina, the micro-implant and associated intraocular features. Statistical analyses of the ERG data showed unchanged functional integrity of retina between eyes with the PLGA-coated CS-based MTX micro-implant and the placebo micro-implant. US analysis showed that micro-implants were stationary throughout the study. SLB, funduscopy and IOP further confirmed that there were no abnormalities in the intraocular physiology. The findings from ERG, US, SLB, funduscopy, and IOP showed no detectable adverse effects caused by our biodegradable micro-implants. These non-invasive techniques appeared to show lack of significant ocular toxicity over time in spite of degradation and changes in morphology of the micro-implants following intraocular implantation. • Sustained release biodegradable PLGA-coated Chitosan-Methotrexate intravitreal micro-implant. • Longitudinal assessment of functional ocular toxicity using non-invasive techniques. • Statistical analysis of functional integrity of retinal response. [ABSTRACT FROM AUTHOR]