19 results on '"Ntafis, Vasileios"'
Search Results
2. PIK3C[delta] expression by fibroblasts promotes triple-negative breast cancer progression
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Gagliano, Teresa, Shah, Kalpit, Gargani, Sofia, Lao, Liyan, Alsaleem, Mansour, Chen, Jianing, Ntafis, Vasileios, Huang, Penghan, Ditsiou, Angeliki, Vella, Viviana, Yadav, Kritika, Bienkowska, Kamila, Bresciani, Giulia, Kang, Kai, Li, Leping, Carter, Philip, Benstead-Hume, Graeme, OHanlon, Timothy, Dean, Michael, Pearl, Frances M.G., Lee, Soo-Chin, Rakha, Emad A., Green, Andrew R., Kontoyiannis, Dimitris L., Song, Erwei, Stebbing, Justin, and Giamas, Georgios
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Genetic engineering -- Analysis ,Idelalisib -- Analysis ,Breast cancer -- Development and progression ,Cancer metastasis -- Development and progression ,RNA -- Analysis ,Health care industry - Abstract
As there is growing evidence for the tumor microenvironment's role in tumorigenesis, we investigated the role of fibroblast-expressed kinases in triple-negative breast cancer (TNBC). Using a high-throughput kinome screen combined with 3D invasion assays, we identified fibroblast-expressed PIK3C[delta] (f- PIK3C[delta]) as a key regulator of cancer progression. Although PIK3C[delta] was expressed in primary fibroblasts derived from TNBC patients, it was barely detectable in breast cancer (BC) cell lines. Genetic and pharmacological gain- and loss-of-function experiments verified the contribution of f-PIK3C[delta] in TNBC cell invasion. Integrated secretomics and transcriptomics analyses revealed a paracrine mechanism via which f-PIK3C[delta] confers its protumorigenic effects. Inhibition of f-PIK3C[delta] promoted the secretion of factors, including PLGF and BDNF, that led to upregulation of NR4A1 in TNBC cells, where it acts as a tumor suppressor. Inhibition of PIK3C[delta] in an orthotopic BC mouse model reduced tumor growth only after inoculation with fibroblasts, indicating a role of f-PIK3C[delta] in cancer progression. Similar results were observed in the MMTV-PyMT transgenic BC mouse model, along with a decrease in tumor metastasis, emphasizing the potential immune-independent effects of PIK3C[delta] inhibition. Finally, analysis of BC patient cohorts and TCGA data sets identified f-PIK3C[delta] (protein and mRNA levels) as an independent prognostic factor for overall and disease-free survival, highlighting it as a therapeutic target for TNBC., Introduction Triple-negative breast cancer (TNBC; [ER.sup.-], [PR.sup.-], [HER2.sup.-]) represents a molecularly diverse and highly heterogeneous subtype of breast cancer (BC) (15%-20%) with a poor prognosis and high rates of recurrence [...]
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- 2020
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3. Harmonisation of education, training and continuing professional development for laboratory animal caretakers, technicians and technologists: Report of the FELASA-EFAT Working Group.
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Abelson, Klas SP, Chambers, Charlie, De La Cueva, Teresa, Fisher, Glyn, Hawkins, Penny, Ntafis, Vasileios, Pohlig, Paul F, Rooymans, T Pim, and Santos, Ana I
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CAREER development ,ANIMAL development ,LABORATORY animals ,TECHNOLOGISTS ,ANIMAL welfare ,EMPLOYEE training facilities - Abstract
Copyright of Laboratory Animals is the property of Sage Publications, Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2023
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4. Validation of a Loop-Mediated Amplification/ISO 6579-Based Method for Analysing Soya Meal for the Presence of Salmonella enterica
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D’Agostino, Martin, Robles, Susana, Hansen, Flemming, Ntafis, Vasileios, Ikonomopoulos, John, Kokkinos, Petros, Alvarez-Ordonez, Avelino, Jordan, Kieran, Delibato, Elisabetta, Kukier, Elżbieta, Sieradzki, Zbigniew, Kwiatek, Krzysztof, Milanov, Dubravka, Petrović, Tamaš, Gonzalez-Garcia, Patricia, Lazaro, David Rodriguez, Jackson, Emily E., Forsythe, Stephen J., O’Brien, Lorna, and Cook, Nigel
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- 2016
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5. The RNA binding protein HuR is a gatekeeper of liver homeostasis
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Subramanian, Pallavi, Gargani, Sofia, Palladini, Alessandra, Chatzimike, Margarita, Grzybek, Michal, Peitzsch, Mirko, Papanastasiou, Anastasios D, Pyrina, Iryna, Ntafis, Vasileios, Gercken, Bettina, Lesche, Mathias, Petzold, Andreas, Sinha, Anupam, Nati, Marina, Thangapandi, Veera Raghavan, Kourtzelis, Ioannis, Andreadou, Margarita, Witt, Anke, Dahl, Andreas, Burkhardt, Ralph, Haase, Robert, Domingues, António Miguel de Jesus, Henry, Ian, Zamboni, Nicola, Mirtschink, Peter, Chung, Kyoung-Jin, Hampe, Jochen, Coskun, Ünal, Kontoyiannis, Dimitris L, and Chavakis, Triantafyllos
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Elavl1 ,Fxr ,Hur ,Nafld ,Nash ,Rna-binding Protein ,Bile Acid ,Hepatocellular Carcinoma ,Lipid Metabolism ,Liver ,Steatosis ,Triglycerides ,digestive system diseases - Abstract
BACKGROUND AND AIMS: Non-alcoholic fatty liver disease (NAFLD) is initiated by steatosis and can progress via fibrosis and cirrhosis to hepatocellular carcinoma (HCC). The RNA binding protein HuR controls RNAs at the posttranscriptional level; hepatocyte HuR has been implicated in the regulation of diet-induced hepatic steatosis. The present study aimed to understand the role of hepatocyte-HuR in NAFLD development and progression to fibrosis and HCC. APPROACH AND RESULTS: Hepatocyte-specific HuR-deficient mice and control HuR-sufficient mice were fed either a normal diet or a NAFLD-inducing diet. Hepatic lipid accumulation, inflammation, fibrosis and HCC development were studied by histology, flow cytometry, quantitative PCR and RNA sequencing. The liver lipidome was characterized by lipidomics analysis and the HuR-RNA interactions in the liver were mapped by RNA immunoprecipitation-sequencing. Hepatocyte-specific HuR-deficient mice displayed spontaneous hepatic steatosis and fibrosis predisposition, compared to control HuR-sufficient mice. On a NAFLD-inducing diet, hepatocyte-specific HuR-deficiency resulted in exacerbated inflammation, fibrosis and HCC-like tumor development. A multi-omic approach, including lipidomics, transcriptomics and RNA-immunoprecipitation sequencing revealed that HuR orchestrates a protective network of hepatic-metabolic and lipid homeostasis-maintaining pathways. Consistently, HuR-deficient livers accumulated, already at steady-state, a triglyceride signature resembling that of NAFLD livers. Moreover, upregulation of Spp1 and its product osteopontin mediated, at least partially, the fibrosis development in hepatocyte-specific HuR deficiency on a NAFLD-inducing diet, as shown by experiments utilizing antibody blockade of osteopontin. CONCLUSIONS: HuR is a gatekeeper of liver homeostasis preventing NAFLD-related fibrosis and HCC, suggesting that the HuR-dependent network could be exploited therapeutically.
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- 2022
6. A complete farm management system based on animal identification using RFID technology
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Voulodimos, Athanasios S., Patrikakis, Charalampos Z., Sideridis, Alexander B., Ntafis, Vasileios A., and Xylouri, Eftychia M.
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- 2010
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7. Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs
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Ntafis, Vasileios, Xylouri, Eftychia, Mari, Viviana, Papanastassopoulou, Maria, Papaioannou, Nikolaos, Thomas, Angelos, Buonavoglia, Canio, and Decaro, Nicola
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- 2012
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8. The RNA binding protein human antigen R is a gatekeeper of liver homeostasis.
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Subramanian, Pallavi, Gargani, Sofia, Palladini, Alessandra, Chatzimike, Margarita, Grzybek, Michal, Peitzsch, Mirko, Papanastasiou, Anastasios D., Pyrina, Iryna, Ntafis, Vasileios, Gercken, Bettina, Lesche, Mathias, Petzold, Andreas, Sinha, Anupam, Nati, Marina, Thangapandi, Veera Raghavan, Kourtzelis, Ioannis, Andreadou, Margarita, Witt, Anke, Dahl, Andreas, and Burkhardt, Ralph
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- 2022
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9. Inactivation of AUF1 in Myeloid Cells Protects From Allergic Airway and Tumor Infiltration and Impairs the Adenosine-Induced Polarization of Pro-Angiogenic Macrophages.
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Gargani, Sofia, Lourou, Niki, Arapatzi, Christina, Tzanos, Dimitris, Saridaki, Marania, Dushku, Esmeralda, Chatzimike, Margarita, Sidiropoulos, Nikolaos D., Andreadou, Margarita, Ntafis, Vasileios, Hatzis, Pantelis, Kostourou, Vassiliki, and Kontoyiannis, Dimitris L.
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MYELOID cells ,MELANOMA ,MACROPHAGES ,RNA-binding proteins ,VASCULAR endothelial growth factors ,VASCULAR remodeling - Abstract
The four isoforms of the RNA-binding protein hnRNPD/AUF1 have been proposed to limit the use of inflammatory mRNAs in innate immune cells. Mice engineered to lack AUF1s in all tissues are sensitive to acute inflammatory assaults; however, they also manifest complex degenerations obscuring assessment of AUF1s' roles in innate immune cells. Here, we restricted a debilitating AUF1 mutation to the mouse myeloid lineage and performed disease-oriented phenotypic analyses to assess the requirement of AUF1s in variable contexts of innate immune reactivity. Contrary to the whole-body mutants, the myeloid mutants of AUF1s did not show differences in their susceptibility to cytokine storms occurring during endotoxemia; neither in type-I cell-mediated reactions driving intestinal inflammation by chemical irritants. Instead, they were resistant to allergic airway inflammation and displayed reductions in inflammatory infiltrates and an altered T-helper balance. The ex-vivo analysis of macrophages revealed that the loss of AUF1s had a minimal effect on their proinflammatory gene expression. Moreover, AUF1s were dispensable for the classical polarization of cultured macrophages by LPS & IFNγ correlating with the unchanged response of mutant mice to systemic and intestinal inflammation. Notably, AUF1s were also dispensable for the alternative polarization of macrophages by IL4, TGFβ and IL10, known to be engaged in allergic reactions. In contrast, they were required to switch proinflammatory macrophages towards a pro-angiogenic phenotype induced by adenosine receptor signals. Congruent to this, the myeloid mutants of AUF1 displayed lower levels of vascular remodeling factors in exudates from allergen exposed lungs; were unable to support the growth and inflammatory infiltration of transplanted melanoma tumors; and failed to vascularize inert grafts unless supplemented with angiogenic factors. Mechanistically, adenosine receptor signals enhanced the association of AUF1s with the Vegfa, Il12b, and Tnf mRNAs to differentially regulate and facilitate the pro-angiogenic switch. Our data collectively demonstrates that AUF1s do not act as general anti-inflammatory factors in innate immune cells but have more specialized roles in regulons allowing specific innate immune cell transitions to support tissue infiltration and remodeling processes. [ABSTRACT FROM AUTHOR]
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- 2022
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10. Phylogenetic and molecular characterization of equine H3N8 influenza viruses from Greece (2003 and 2007): Evidence for reassortment between evolutionary lineages
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Kanellos Theo, Ntafis Vasileios, Fragkiadaki Eirini, Bountouri Maria, and Xylouri Eftychia
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Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background For first time in Greece equine influenza virus infection was confirmed, by isolation and molecular analysis, as the cause of clinical respiratory disease among unvaccinated horses during 2003 and 2007 outbreaks. Methods Equine influenza virus (EIV) H3N8 was isolated in MDCK cells from 30 nasal swabs from horses with acute respiratory disease, which were tested positive by Directigen Flu A. Isolation was confirmed by haemagglutination assay and RT-PCR assay of the M, HA and NA gene. Results HA sequences of the Greek isolates appeared to be more closely related to viruses isolated in early 1990s in Europe. These results suggested that viruses with fewer changes than those on the main evolutionary lineage may continue to circulate. On the other hand, analysis of deduced NA amino acid sequences were more closely related to viruses isolated in outbreaks in Europe and Asia during 2003-2007. Phylogenetic analysis characterized the Greek isolates as a member of the Eurasian lineage by the haemagglutinin (HA) protein alignment, but appeared to be a member of the Florida sublineage clade 2 by the neuraminidase (NA) protein sequence suggesting that reassortment might be a possible explanation. Conclusion Our findings suggest that the Greek strains represent an example of "frozen evolution" and probably reassortment between genetically distinct co-circulated strains. Therefore expanding current equine influenza surveillance efforts is a necessity.
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- 2011
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11. Validation of a Loop-Mediated Amplification/ISO 6579-Based Method for Analysing Soya Meal for the Presence of Salmonella enterica.
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D'Agostino, Martin, Robles, Susana, Hansen, Flemming, Ntafis, Vasileios, Ikonomopoulos, John, Kokkinos, Petros, Alvarez-Ordonez, Avelino, Jordan, Kieran, Delibato, Elisabetta, Kukier, Elżbieta, Sieradzki, Zbigniew, Kwiatek, Krzysztof, Milanov, Dubravka, Petrović, Tamaš, Gonzalez-Garcia, Patricia, Lazaro, David, Jackson, Emily, Forsythe, Stephen, O'Brien, Lorna, and Cook, Nigel
- Abstract
An alternative method for detection of Salmonella spp. in animal feed, based on the use of loop-mediated amplification (LAMP) in conjunction with a standard culturing procedure, was compared with the standard ISO 6579 as reference method, using soya meal as the test matrix. In the method comparison study, the sensitivities for both the alternative and reference methods were 100 %. The relative level of detection was 1.000. Tested against 100 Salmonella and 30 non- Salmonella strains, the LAMP-based method was 99 % inclusive and 100 % exclusive. The interlaboratory trial involved ten laboratories from eight European countries, testing eight samples at three contamination levels: 0 cfu/100 g, 1-5 cfu/100 g and 14-68 cfu/100 g. The trial specificity, or percentage correct identification of uncontaminated samples, was 96.3 % for both the reference methods and the LAMP/ISO 6579 alternative method, thus demonstrating its suitability for adoption as a procedure for rapid identification of Salmonella uncontaminated soya meal. [ABSTRACT FROM AUTHOR]
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- 2016
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12. Canine coronavirus, Greece. Molecular analysis and genetic diversity characterization.
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Ntafis, Vasileios, Mari, Viviana, Decaro, Nicola, Papanastassopoulou, Maria, Pardali, Dimitra, Rallis, Timoleon S., Kanellos, Theophanis, Buonavoglia, Canio, and Xylouri, Eftychia
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CORONAVIRUS diseases , *CORONAVIRUS genetics , *ETIOLOGY of diseases , *HEALTH outcome assessment , *MOLECULAR probes , *GENETIC recombination , *POLYMERASE chain reaction , *DIAGNOSIS , *VIRUSES - Abstract
Abstract: Canine coronavirus (CCoV) is an etiologic agent of diarrhea in dogs and is known to have spread worldwide. Mild disease or asymptomatic carriage are probably in many cases common outcomes of infection. To date, two different genotypes of CCoV are known, CCoV type I (CCoV-I) and CCoV type II (CCoV-II). CCoV type II is divided in two subtypes, CCoV-IIa (classical strains) and CCoV-IIb, with CCoV-IIb emerging as a result of a putative recombination between CCoV-IIa and transmissible gastroenteritis virus (TGEV). The aim of the present study was to investigate the presence of CCoV in Greece and to genetically analyze the circulating strains. Between December 2007 and December 2009, 206 fecal samples were collected from dogs with diarrhea from kennels, pet shops and veterinary clinics of different country regions. RT-PCR and real time RT-PCR assays were used for CCoV detection and characterization. CCoV was identified in 65.1% of the dogs presenting diarrhea, being more frequently detected in animals younger than 3months old and in animals housed in groups. In 47% of the positive samples more than one CCoV genotype/subtype were detected, with triple CCoV-I/CCoV-IIa/CCoV-IIb infections being identified for the first time. Molecular and phylogenetic analysis revealed that CCoV-I Greek strains share low genetic relatedness to each other and to the prototype CCoV-I strains in the 5’ end of the S gene. Moreover, a divergent CCoV-IIa strain was identified. The circulation of highly variable CCoV-I and CCoV-IIb emerging strains, as well as the detection of the divergent strain, raise concerns on the importance of these new strains as primary pathogens of diarrhoeic syndromes diagnosed in dogs. [Copyright &y& Elsevier]
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- 2013
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13. Characterization of Canine parvovirus 2 variants circulating in Greece.
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Ntafis, Vasileios, Xylouri, Eftychia, Kalli, Iris, Desario, Costantina, Mari, Viviana, Decaro, Nicola, and Buonavoglia, Canio
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CANINE parvovirus ,ENTERITIS ,DOG diseases ,VIRAL variation ,POLYMERASE chain reaction ,RADIOLIGAND assay - Abstract
The article discusses a study seeking to characterize Canine parvovirus 2 (CPV-2) in Greece. CPV-2 is an agent that causes hemorrhagic enteritis in dogs. Standard polymerase chain reaction (PCR) was used to detect CPV-2 while minor groove binder prove assays were used to identify genetic variants. The study found that CPV-2a strains are the most common variant in the country.
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- 2010
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14. An outbreak of Canine coronavirus in puppies in a Greek kennel.
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Ntafis, Vasileios, Mari, Viviana, Danika, Stefania, Fragkiadaki, Eftychia, and Buonavoglia, Canio
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CORONAVIRUSES ,CORONAVIRUS diseases ,KENNELS ,DOG diseases ,DISEASES ,PUPPIES ,GASTROINTESTINAL diseases - Abstract
The article discusses research on the outbreak of coronavirus in a Greek dog's puppies. It references a study by Stefania Danika and colleagues, published in the 2010 issue of the "Journal of the Veterinary Diagnostic Investigation." The researchers examined the association of the virus with the development of gastroenteritis in dogs. They found that the other effects of the virus include dehydration, anorexia and diarrhea.
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- 2010
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15. Faecal shedding of Mycobacterium avium subspecies paratuberculosis reduces before parturition in sheep?
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Mataragka, Antonia, Leousi, Elisavet, Liandris, Emmanouil, Ntafis, Vasileios, Leontides, Leonidas, Aggelidou, Elisavet, Bossis, Ioannis, Triantaphyllopoulos, Kostas A., Theodoropoulou, Ioanna, and Ikonomopoulos, John
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PARATUBERCULOSIS , *MYCOBACTERIUM avium , *PARTURITION , *SHEEP , *POLYMERASE chain reaction , *ENZYME-linked immunosorbent assay - Abstract
Paratuberculosis is a disease affecting mainly ruminants, and it is caused by Mycobacterium avium subspecies paratuberculosis (MAP). Control of the disease relies primarily on test-and-removal that is not easy to apply effectively for reasons associated with its pathogenesis. Therefore the aim of this preliminary study was to investigate whether positivity of sheep to MAP-specific antibody and DNA, detected by ELISA and faecal real time polymerase chain reaction (PCR) respectively, varies between specific stages of breeding i.e. before and after parturition, and before the mating season. Rectal faeces (n = 120) and whole blood (n = 120) samples were collected from a flock of sheep with no record of clinical paratuberculosis. The sheep under study are maintained throughout the year, in isolated confinement with no access to pasture or other animals, and a continuously monitored diet. The proportion of positive animals to real time PCR before parturition (13.9%) was found to be significantly lower (χ 2 = 10.67, P = 0.0015 ) than the proportion of positive animals after parturition (59.5%), and before the mating season (47.6%). The proportion of positive animals after parturition and before the mating season did not differ significantly. PCR analysis of one sample collected from each animal after parturition, allowed detection of more than 71% of the reactors. In conclusion, faecal shedding of MAP detected by real time PCR seems to be decreased before parturition, which probably makes the specific period less suitable for detection of shedders in sheep with no clinical signs of paratuberculosis. [ABSTRACT FROM AUTHOR]
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- 2017
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16. Lung tumor MHCII immunity depends on in situ antigen presentation by fibroblasts.
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Kerdidani D, Aerakis E, Verrou KM, Angelidis I, Douka K, Maniou MA, Stamoulis P, Goudevenou K, Prados A, Tzaferis C, Ntafis V, Vamvakaris I, Kaniaris E, Vachlas K, Sepsas E, Koutsopoulos A, Potaris K, and Tsoumakidou M
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- Animals, Apoptosis, Cancer-Associated Fibroblasts metabolism, Cancer-Associated Fibroblasts pathology, Carrier Proteins metabolism, Disease Models, Animal, Humans, Interferon-gamma metabolism, Lung Neoplasms metabolism, Lung Neoplasms pathology, Lymphocyte Activation, Lymphocyte Count, Lymphocytes, Tumor-Infiltrating immunology, Lymphocytes, Tumor-Infiltrating metabolism, Lymphocytes, Tumor-Infiltrating pathology, Mice, Mitochondrial Proteins metabolism, Single-Cell Analysis, T-Lymphocyte Subsets immunology, T-Lymphocyte Subsets metabolism, Transcriptome, Tumor Microenvironment immunology, Antigen Presentation immunology, Antigens, Neoplasm immunology, Cancer-Associated Fibroblasts immunology, Histocompatibility Antigens Class II immunology, Lung Neoplasms immunology
- Abstract
A key unknown of the functional space in tumor immunity is whether CD4 T cells depend on intratumoral MHCII cancer antigen recognition. MHCII-expressing, antigen-presenting cancer-associated fibroblasts (apCAFs) have been found in breast and pancreatic tumors and are considered to be immunosuppressive. This analysis shows that antigen-presenting fibroblasts are frequent in human lung non-small cell carcinomas, where they seem to actively promote rather than suppress MHCII immunity. Lung apCAFs directly activated the TCRs of effector CD4 T cells and at the same time produced C1q, which acted on T cell C1qbp to rescue them from apoptosis. Fibroblast-specific MHCII or C1q deletion impaired CD4 T cell immunity and accelerated tumor growth, while inducing C1qbp in adoptively transferred CD4 T cells expanded their numbers and reduced tumors. Collectively, we have characterized in the lungs a subset of antigen-presenting fibroblasts with tumor-suppressive properties and propose that cancer immunotherapies might be strongly dependent on in situ MHCII antigen presentation., Competing Interests: Disclosures: The authors declare no competing interests exist., (© 2022 Kerdidani et al.)
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- 2022
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17. European surveillance for pantropic canine coronavirus.
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Decaro N, Cordonnier N, Demeter Z, Egberink H, Elia G, Grellet A, Le Poder S, Mari V, Martella V, Ntafis V, von Reitzenstein M, Rottier PJ, Rusvai M, Shields S, Xylouri E, Xu Z, and Buonavoglia C
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- Animal Structures virology, Animals, Cluster Analysis, Coronavirus Infections epidemiology, Coronavirus Infections virology, Dogs, Europe epidemiology, Genetic Variation, Membrane Glycoproteins genetics, Molecular Sequence Data, Phylogeny, Prevalence, Sequence Analysis, DNA, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins genetics, Coronavirus Infections veterinary, Coronavirus, Canine isolation & purification, Dog Diseases epidemiology, Dog Diseases virology
- Abstract
Highly virulent pantropic canine coronavirus (CCoV) strains belonging to subtype IIa were recently identified in dogs. To assess the distribution of such strains in Europe, tissue samples were collected from 354 dogs that had died after displaying systemic disease in France (n = 92), Hungary (n = 75), Italy (n = 69), Greece (n = 87), The Netherlands (n = 27), Belgium (n = 4), and Bulgaria (n = 1). A total of 124 animals tested positive for CCoV, with 33 of them displaying the virus in extraintestinal tissues. Twenty-four CCoV strains (19.35% of the CCoV-positive dogs) detected in internal organs were characterized as subtype IIa and consequently assumed to be pantropic CCoVs. Sequence and phylogenetic analyses of the 5' end of the spike protein gene showed that pantropic CCoV strains are closely related to each other, with the exception of two divergent French viruses that clustered with enteric strains.
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- 2013
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18. Phylogenetic and molecular characterization of equine H3N8 influenza viruses from Greece (2003 and 2007): evidence for reassortment between evolutionary lineages.
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Bountouri M, Fragkiadaki E, Ntafis V, Kanellos T, and Xylouri E
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- Animals, Cell Line, Cluster Analysis, Dogs, Greece, Hemagglutination Inhibition Tests, Influenza A Virus, H3N8 Subtype genetics, Molecular Sequence Data, Nasal Mucosa virology, Phylogeny, RNA, Viral genetics, Reassortant Viruses genetics, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Virus Cultivation, Horse Diseases virology, Horses virology, Influenza A Virus, H3N8 Subtype isolation & purification, Orthomyxoviridae Infections virology, Reassortant Viruses isolation & purification
- Abstract
Background: For first time in Greece equine influenza virus infection was confirmed, by isolation and molecular analysis, as the cause of clinical respiratory disease among unvaccinated horses during 2003 and 2007 outbreaks., Methods: Equine influenza virus (EIV) H3N8 was isolated in MDCK cells from 30 nasal swabs from horses with acute respiratory disease, which were tested positive by Directigen Flu A. Isolation was confirmed by haemagglutination assay and RT-PCR assay of the M, HA and NA gene., Results: HA sequences of the Greek isolates appeared to be more closely related to viruses isolated in early 1990s in Europe. These results suggested that viruses with fewer changes than those on the main evolutionary lineage may continue to circulate. On the other hand, analysis of deduced NA amino acid sequences were more closely related to viruses isolated in outbreaks in Europe and Asia during 2003-2007. Phylogenetic analysis characterized the Greek isolates as a member of the Eurasian lineage by the haemagglutinin (HA) protein alignment, but appeared to be a member of the Florida sublineage clade 2 by the neuraminidase (NA) protein sequence suggesting that reassortment might be a possible explanation., Conclusion: Our findings suggest that the Greek strains represent an example of "frozen evolution" and probably reassortment between genetically distinct co-circulated strains. Therefore expanding current equine influenza surveillance efforts is a necessity.
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- 2011
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19. Outbreak of canine norovirus infection in young dogs.
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Ntafis V, Xylouri E, Radogna A, Buonavoglia C, and Martella V
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- Animals, Dogs virology, Feces virology, Greece, Molecular Sequence Data, Norovirus genetics, Phylogeny, RNA, Viral analysis, Reverse Transcriptase Polymerase Chain Reaction, Caliciviridae Infections epidemiology, Caliciviridae Infections veterinary, Caliciviridae Infections virology, Disease Outbreaks veterinary, Dog Diseases epidemiology, Dog Diseases virology, Gastroenteritis epidemiology, Gastroenteritis veterinary, Gastroenteritis virology, Norovirus isolation & purification
- Abstract
An outbreak of norovirus (NoV) infection was identified in a kennel. Sequence analysis of a short fragment in the polymerase complex indicated the clonal origin of the strains, which were similar to the prototype canine NoV strain GIV.2/Bari/170/07-4/ITA (94.7% nucleotide identity). The findings demonstrate that canine NoV circulates in dogs in Greece and that it can spread easily across a group of animals.
- Published
- 2010
- Full Text
- View/download PDF
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