7 results on '"O'Shea, Sheila"'
Search Results
2. Insulin enhances glucose-stimulated insulin secretion in healthy humans
- Author
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Bouche, Clara, Lopez, Ximena, Fleischman, Amy, Cypess, Aaron M., O'Shea, Sheila, Stefanovski, Darko, Bergman, Richard N., Rogatsky, Eduard, Stein, Daniel T., Kahn, C. Ronald, Kulkarni, Rohit N., and Goldfine, Allison B.
- Published
- 2010
3. Investigation of meta-linguistic and meta-cognitive intervention to improve comprehension of coordinating conjunctions
- Author
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O'Shea, Sheila and Murphy, Carol-Anne
- Subjects
primary language comprehension difficulties ,children ,oral language comprehension - Abstract
non-peer-reviewed Background: There is a limited evidence base available in the area of oral language comprehension for children with primary language comprehension difficulties, with existing research which demonstrates treatment effectiveness largely focusing on pre-school or older school age children. Objectives: This pilot study aims to investigate the efficacy of individually-tailored intervention and strategies to improve the oral comprehension skills of a child with primary language impairment aged 7 years 11 months. Methodology: This intervention centred on a meta-linguistic approach with strategies tailored to match deficits of the individual participating in the study. A single case study design was chosen due to the heterogeneity of the population under investigation, allowing specific grammatical targets to be identified and treated using shapes and colours (Shape Coding, Ebbels 2007) to make the grammatical rules of English explicit. Rehearsal and visualisation strategies were employed to help the participant with working memory challenges. Comprehension monitoring strategies were explicitly taught to facilitate the participant to recognise when and how to ask for help. Results: Non-parametric analysis comparing the child???s performance on pre and post assessment of treated and untreated measures demonstrated a statistically significant improvement (p=.008* 2-tailed) in comprehension of treated coordinating conjunction ???neither/nor??? with no significant change in untreated structures. Spontaneous use of comprehension monitoring, and rehearsal and visualisation strategies were evident toward the end of therapy. Comprehension: These findings provide preliminary evidence to support the use of a two-pronged approach in treating oral comprehension of younger school-aged children with primary language impairment.
- Published
- 2013
4. Biomarkers in Fasting Serum to Estimate Glucose Tolerance, Insulin Sensitivity, and Insulin Secretion.
- Author
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Goldfine, Allison B., Gerwien, Robert W., Kolberg, Janice A., O'Shea, Sheila, Hamren, Sarah, Hein, Glenn P., Xu, Xiaomei M., and Patti, Mary Elizabeth
- Published
- 2011
- Full Text
- View/download PDF
5. Hyperinsulinemia Increases the Metabolic Clearance Rate of C-peptide.
- Author
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Cypess, Aaron M., Lopez, Ximena, O'shea, Sheila, Rogatsky, Eduard, Stein, Daniel T., and Goldfine, Allison B.
- Subjects
C-peptide ,METABOLIC clearance rate ,HYPERINSULINISM ,PROINSULIN ,GLUCOSE ,CHROMATOGRAPHIC analysis ,PANCREATIC beta cells - Abstract
Endogenous insulin secretion is frequently estimated by measuring C-peptide concentrations based on the assumptions of a 1:1 molar ratio of insulin:C-peptide secretion and constant C-peptide clearance rates. Recent evidence suggests that C-peptide clearance is altered in the presence of hyperinsulinemia. Using 13C-labeled C-peptide, we measured C-peptide clearance rates in 5 healthy volunteers with no family history of diabetes, 2M/3F, age 29 ± 2y, BMI 24.4 ± 1.4 kg/m², FBS 90± 2 mg/dL, and A1c 5.0 ±0.1% in the setting of 4 hours of saline infusion (low insulin) or during a euglycemic-hyper-insulinemia clamp at 2.0 mU Novolog (NovoNordisk) insulin/kg*min. 13C-C-peptide was infused on both study days as a primed (33 pmol/kg over 2 min) continuous infusion at 1.5 pmol/kg*min. Total insulin was measured by RIA, and both 13C-C-peptide and endogenous C-peptide were measured via 2-dimensional liquid chromatography-mass spectrometry. Glucose levels were similar on both study days, and subjects were insulin-sensitive, with a glucose utilization (M) of 10.8 ±1.2 mg/kg*min. Insulin levels were at steady-state during hours 2-4 of the hyperinsulinemic clamp (184 ±16 µU/mL). Compared with the saline infusions, during hyperinsulinemia the 13C-C-peptide levels achieved were 11% lower (1.25 ± 0.13 vs. 1.11 ± 0.12 ng/mL, p<0.05), and the metabolic clearance rate (MCR) of C-peptide was increased by 9% (from 229 ± 40 to 248 ± 34 mL/min, p<0.05). These findings demonstrate that C-peptide clearance is increased over the range of hyperinsulinemia studied in clamps and suggest that altered C-peptide clearance could confound estimates of β-cell function during hyperinsulinemia. [ABSTRACT FROM AUTHOR]
- Published
- 2007
6. Insulin Sensitivity Remains Stable in Serial Studies of Healthy Patients.
- Author
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Cypess, Aaron M., Bouche, Clara, Fleischman, Amy, O'Shea, Sheila, and Goldfine, Allison B.
- Subjects
INSULIN resistance ,BODY weight ,PHYSICAL fitness ,DIABETES ,BODY mass index ,BLOOD sugar - Abstract
The standard for measuring whole-body insulin sensitivity is via euglycemic-hyperinsulinemic clamp. In a given individual, it can vary significantly from hour to hour in response to interventions such as exercise, diet, drugs, and stressors. Over longer periods of time, fluctuations in body weight, physical activity, and progressive age, might alter insulin sensitivity. The variability of insulin sensitivity over time in persons at low risk for progression to diabetes is incompletely understood. We measured insulin sensitivities in 4 healthy volunteers with no family history of diabetes, 2M/2F, age 25±6y, BMI 22 ± 2 kg/m², FBS 90 ± 4 mg/dL, and A1c 5.0 ±0.2%. Each person was assessed 2-3 times over the course of 3.5 years via a 4-hour euglycemic-hyperinsulinemic clamp (2.0 mU/kg*min). Insulin levels were at steady-state during the fourth hour of the clamps as measured by RIA (173.6 ± 9.5 µU/mL). At this insulin level, hepatic glucose production is fully suppressed, so glucose utilization (M) was estimated by the glucose infusion rate, which initially was 11.6 ± 0.6 mg/kg*min. M did not change significantly over time, being 11.8 ± 1.2 mg/kg*min at the final evaluation. Over the study period, several metabolic indices varied substantially from the initial measurements in any given subject, with the following mean coefficients of variation (CV): BMI 4.9 ± 1.2%, FBS 4.4 ± 1.4%, fasting plasma insulin 45.0 ± 10.8%, HOMA-IR 46.9 ± 9.8%, total cholesterol 8.2 ± 3.1%, triglycerides 45.5 ± 6.0%, HDL 16.0 ± 5.8%, and LDL 16.4 ± 2.1%. We demonstrated a mean CV for M of 8.1 ± 0.2%. Since steady-state insulin levels achieved during the clamps also varied, we related whole-body insulin sensitivity to exogenous insulin via M/I* 100, whose cohort average at baseline was 14.6 ± 2.2 and at the end of the study 17.5 ± 1.9 (p=ns). The mean CV of the M/I* 100 for each of the subjects, over all of the clamps, was 12.2 ± 6.1%. By comparison, the initial and final HOMA-IR were, respectively, 1.1 ± 0.3 and 1.8 ± 0.4 (p=ns), and the mean CV over all measures was 55.1±13.3%. These results suggest a high degree of reproducibility of clamp-measured insulin sensitivity and that in healthy individuals at low risk for diabetes, insulin sensitivity does not change substantially or disproportionately to variations in other metabolic parameters over the course of several years. The considerably larger CV for HOMA-IR supports that it is not a robust index of insulin sensitivity when assessing individuals or smaller cohorts. [ABSTRACT FROM AUTHOR]
- Published
- 2007
7. Exogenous insulin enhances glucose-stimulated insulin response in healthy humans independent of changes in free fatty acids.
- Author
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Lopez X, Cypess A, Manning R, O'Shea S, Kulkarni RN, and Goldfine AB
- Subjects
- Adult, Blood Glucose metabolism, Female, Humans, Insulin pharmacology, Insulin Secretion, Insulin-Secreting Cells drug effects, Male, Single-Blind Method, Fatty Acids, Nonesterified blood, Glucose pharmacology, Insulin metabolism, Insulin-Secreting Cells metabolism
- Abstract
Context: Islet β-cells express both insulin receptors and insulin signaling proteins. Recent studies suggest insulin signaling is physiologically important for glucose sensing., Objective: Preexposure to insulin enhances glucose-stimulated insulin secretion (GSIS) in healthy humans. We evaluated whether the effect of insulin to potentiate GSIS is modulated through regulation of free fatty acids (FFA)., Design and Setting: Subjects were studied on three occasions in this single-site study at an academic institution clinical research center., Patients: Subjects included nine healthy volunteers., Interventions: Glucose-induced insulin response was assessed on three occasions after 4 h saline (low insulin/sham) or isoglycemic-hyperinsulinemic (high insulin) clamps with or without intralipid and heparin infusion, using B28 Asp-insulin that could be distinguished from endogenous insulin immunologically. During the last 80 min of all three clamps, additional glucose was administered to stimulate insulin secretion (GSIS) with glucose concentrations maintained at similar concentrations during all studies., Main Outcome Measure: β-Cell response to glucose stimulation was assessed., Results: Preexposure to exogenous insulin increased the endogenous insulin-secretory response to glucose by 32% compared with sham clamp (P = 0.001). This was accompanied by a drop in FFA during hyperinsulinemic clamp compared with the sham clamp (0.06 ± 0.02 vs. 0.60 ± 0.09 mEq/liter, respectively), which was prevented during the hyperinsulinemic clamp with intralipid/heparin infusion (1.27 ± 0.17 mEq/liter). After preexposure to insulin with intralipid/heparin infusion to maintain FFA concentration, GSIS was 21% higher compared with sham clamp (P < 0.04) and similar to preexposure to insulin without intralipid/heparin (P = 0.2)., Conclusions: Insulin potentiates glucose-stimulated insulin response independent of FFA concentrations in healthy humans.
- Published
- 2011
- Full Text
- View/download PDF
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