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Induced resistance (IR) based on elicitors application aims to strengthen plant defenses, rather than directly targeting pathogens. These compounds are less toxic than conventional pesticides and are useful in reducing their use. Strategies based on COS–OGA, Swinglea glutinosa, and low copper doses were tested in this three-year (2021–2023) field investigation against grapevine downy (DM) and powdery (PM) mildew. Their effectiveness was compared to copper, sulfur, untreated, and water-sprayed plants in a naturally infected vineyard. Combined treatments provided higher levels of protection. COS–OGA at 2 L ha−1 combined with low copper doses and an adjuvant protected canopy and production from both DM and PM at the same level as copper and farm application (based on copper and sulfur). In favorable seasons, 2023 for DM and 2022 for PM, this strategy reduced the DM McKinney Index compared to untreated plants by 74% and 39% on leaves and bunches, respectively. Reductions observed for PM were 85% on leaves and 46% on bunches. S. glutinosa showed reductions only in one assessment against PM on bunches. This work validates IR-based DM and PM management strategies under field conditions and paves the way toward concrete reduction in copper and sulfur use in vineyards. [ABSTRACT FROM AUTHOR]

Soil water availability during the vine growth cycle can affect yield and grape quality. The objective was to evaluate the effect of soil water holding capacity (AWC) and available soil water (ASW) throughout the growing cycle on the nutritional status, vigor, production, and composition of grapes and wine. The study was conducted in the municipality of Uruñuela in the DOCa Rioja (Spain). The soils of four rainfed vineyard plots were characterized to determine AWC and its impacts on vine, grape, and wine composition. The N, P, and K foliar content, vigor, grape yield, berry weight, and composition of must and wine were analyzed in those vineyard plots during the period 2010–2014. The ASW was simulated in each plot and each year analyzed, considering the soil properties and the weather conditions, after model calibration in one plot in which soil water content was registered. The results showed that AWC influenced ASW along the growing cycle, so vines suffered from water stress in some periods of the vegetative cycle. Plots with higher AWC had higher ASW from fruit set to ripening and lower water stress during this period, which explains the higher N, P, and K foliar content, vigor and grape yield, and lower polyphenol and anthocyanin content in grapes and wines. The period where water availability had the most influence on the quality of the grapes was from veraison to ripening, during which ASW increased berry weight and acidity and decreased anthocyanins and polyphenolic compounds. [ABSTRACT FROM AUTHOR]

Greenhouse gas (GHG) emissions are influenced by physical, chemical, biological, and anthropogenic factors. The objective of the study is to carry out a comprehensive analysis of the emissions of three important agricultural GHGs (CO2, N2O, and CH4) in both rows and alleys of a vineyard (1) and to understand their interactions with the agricultural operations carried out in the experimental plot, namely tillage, inter-row management, application of mineral and organic fertilizers, and irrigation and pruning, as well as the agroclimatic conditions of the plot (2). The study was conducted in a vineyard of Vitis vinifera L. cv. Tempranillo blanco in the DOCa. Rioja grape-growing region, during 2017, 2018, and 2019. Cumulative CO2 emissions were highest in 2018, reaching 934.7 ± 66.5 kg ha−1 day−1 in the alleys and 926.8 ± 76.5 kg ha−1 day−1 in the rows, in agreement with the wetter year and organic matter decomposition at the end of 2017. N2O emissions during the three-year study were mainly affected by mineral fertilizer application, with increases of 41.1 g ha−1 day−1 in the alleys and 49.3 g ha−1 day−1 in the rows during 2018, and 33.1 g ha−1 day−1 in the alleys and 39.6 g ha−1 day−1 in the rows in 2019. Regarding CH4, anaerobic soil conditions in 2018 (the year with the highest rainfall) led to the highest flux of CH4 emissions to the atmosphere, with 215.5 ± 51.0 g ha−1 day−1 in the corridors and 238.4 ± 54.9 g ha−1 day−1 in the rows. This study emphasizes the complex interplay of physical, chemical, biological, and human-related factors affecting GHG emissions in viticultural soils. Understanding these dynamics is essential for developing sustainable vineyard practices that minimize emissions and contribute to climate change mitigation. [ABSTRACT FROM AUTHOR]

BACKGROUND: Grape aromas are formed by a great number of volatile compounds. Methyl jasmonate (MeJ) and urea (Ur) foliar applications have been studied to improve grape quality, but their combined application has never been studied. RESULTS: In both seasons, MeJ application enhanced terpenoids and C6 compounds synthesis, though decreased alcohols content. Moreover, MeJ + Ur treatment reduced benzenoids and alcohols and did not affect C13‐norisoprenoids content. However, there was no clear effect of these treatments on the rest of the volatile compounds. Multifactorial analysis showed a season effect on all volatile compounds, except terpenoids. Discriminant analysis showed a good separation among samples under treatment criterion. The great effect of MeJ treatment on terpenoids was probably due to this elicitor influencing their biosynthesis. CONCLUSION: Season has a strong influence on grapes aromatic composition since it affects all volatile compound families except terpenoids. MeJ foliar application enhanced terpenoids, C13‐norisoprenoids and C6 compounds synthesis, whereas decreased alcohols content; however, MeJ + Ur foliar treatment did not affect C13‐norisoprenoids and C6 compounds, and decreased benzenoids and alcohols grape compounds. Therefore, no synergistic effect was observed between Ur and MeJ on grape volatile compounds biosynthesis. Foliar application of MeJ seems to be sufficient to improve the aromatic quality of grapes. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Viticulture is highly dependent on phytochemicals to maintain good vineyard health. However, to reduce their accumulation in the environment, green regulations are driving the development of eco-friendly strategies. In this respect, seaweeds have proven to be one of the marine resources with the highest potential as plant protective agents, representing an environmentally-friendly alternative approach for sustainable wine production. The current work follows an interdisciplinary framework to evaluate the capacity of Ulva ohnoi and Rugulopteryx okamurae seaweeds to induce defense mechanisms in grapevine plants. To our knowledge, this is the first study to evaluate Rugulopteryx okamurae as a biostimulator . This macroalgae is relevant since it is an invasive species on the Atlantic and Mediterranean coast causing incalculable economic and environmental burdens. Four extracts (UL1, UL2, RU1 and RU2 developed from Ulva and Rugulopteryx, respectively) were foliar applied to Tempranillo plants cultivated under greenhouse conditions. UL1 and RU2 stood out for their capacity to induce defense genes, such as a PR10, PAL, STS48 and GST1, mainly 24 hours after the first application. The increased expression level of these genes agreed with i) an increase in trans-piceid and trans-resveratrol content, mainly in the RU2 treated leaves, and, ii) an increase in jasmonic acid and decrease in salicylic acid. Moreover, an induction of the activity of the antioxidant enzymes was observed at the end of the experiment, with an increase in superoxide dismutase and catalase in the RU2-treated leaves in particular. Interestingly, while foliar fungal diversity was not influenced by the treatments, alga extract amendment modified fungal composition, RU2 application enriching the content of various groups known for their biocontrol activity. Overall, the results evidenced the capacity of Rugulopteryx okamurae for grapevine biostimulation, inducing the activation of several secondary metabolite pathways and promoting the abundance of beneficial microbiota involved in grapevine protection. While further studies are needed to unravel the bioactive compound(s) involved, including conducting field experiments etc., the current findings are the first steps towards the inclusion of Rugulopteryx okamurae in a circular scheme that would reduce its accumulation on the coast and benefit the viticulture sector at the same time. [ABSTRACT FROM AUTHOR]

In recent years, foliar application of elicitors to the vineyard has been increasingly used, in particular, elicitation with methyl jasmonate (MeJ). However, due to the high cost of this compound, it is necessary to find a form of application in which the amount to be used is considerably reduced. Therefore, the aim of this work was study for the first time the influence of foliar application of nanoparticles doped with MeJ (ACP-MeJ) and foliar application of methyl jasmonate (MeJ), using a dose of 1 mM versus 10 mM, respectively, on volatile composition of Tempranillo grapes during two consecutive vintages. Grape volatile composition was determined by SPME-GC-MS. The obtained results reveal that MeJ application increased the concentration of terpenoids, and total C6 compounds in 2019 and 2020, and C13 norisoprenoids in 2019. In addition, ACP-MeJ enhanced the amount of terpenoids, and benzenoids in 2020. These are encouraging results considering that the ACP-MeJ dose was 10 times lower than that of MeJ. Therefore, the foliar application of MeJ supported on nanoparticles could be a tool in order to improve grape volatile composition, favoring a more viable and sustainable viticulture. [ABSTRACT FROM AUTHOR]

Abstract: BACKGROUND: Carignan noir is one of the minor and ancient varieties from the Chilean wine scenario that has had a resurgence as a result of to its rediscovered oenological potential when cultivated under the conditions of the interior dryland area. Under these growing conditions Carignan noir wines stand out as fruit driven and fresh compared to those originating from other growing areas. On the other hand, it is well known that wine aroma composition depends mainly on variety, viticultural management and winemaking. Thus, the present study aimed to investigate the volatile composition of wines from Carignan noir grapes originating from ungrafted and grafted onto País grapevines, in ten sites of the Maule Valley (Chile) during two consecutive vintages. RESULTS: Higher alcohols were the most abundant volatile compounds, whereas ethyl esters and ß‐damascenone were the most odoriferous compounds according to their odorant activity value. The dominant factor in Carignan noir wine volatile composition was season, whereas rootstock did not have a significant effect in differentiating the wines. In terms of climate, cold nights during the month prior to harvest correlated with higher linalool, α‐terpineol and trans‐3‐hexen‐1‐ol content. The biologically effective degree‐days index was inversely correlated with isoamyl acetate and 2‐phenylethyl acetate content. Huglin's heliothermal and the average mean temperature of the warmest month indices were inversely correlated with ethyl vanillate, ethyl hexanoate and ethyl octanoate content. CONCLUSION: Site climate conditions were related to the accumulation of certain wine volatile compounds. This information may be useful for devising new Carignan noir vineyard management strategies when aiming to improve wine quality or adjust site selection approaches to maximize grape/wine volatile composition according to wine style. © 2018 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

Cover crop use in vineyards can affect both vine vigor and must and wine quality because of the competition for soil nutrients and water. Our objective was to study the short-term effects of a cover crop on the nitrate (NO3−)–nitrogen (N) availability throughout the grapevine vegetative cycle, the grapevine and cover crop N uptake, and the yield and must quality. By short-term effects we mean the first crop cycle after planting the cover crop. The experiment was set in 2009 on a cv. Tempranillo vineyard planted in aOxyaquic Xerorthentsoil. The soil had not been fertilized with N since 2000, and two types of soil management were studied: (1) conventional tillage (CT) and (2) barley (Hordeum vulgareL.) cover crop (B). Soil samples were taken in March (bud break), June (bloom), July (setting), and August (veraison) of 2009, and the extractable NO3−-N was determined. At bloom and veraison, N contents in both blade and petiole were determined. At bloom the grapevine N uptake was estimated using the aerial parts (leaves, shoots, and bunches), and for the cover crop N uptake was determined. Total yield, bunch, and shoot weight as well as must anthocyanin and polyphenol contents were determined. Soil NO3−-N availability decreased in the cover crop from June until August, with the reduction being similar to the cover crop N uptake. Also N contents in both petiole and blade decreased in the cover crop at veraison. Regarding must quality, the cover crop increased the anthocyanin content. The reduction of soil NO3−-N availability throughout the vegetative cycle of grapevine caused a reduction in both N nutritional status and grapevine vigor, and therefore cover crops could affect must quality in the first harvest after the cover crop was sown. [ABSTRACT FROM AUTHOR]

Nitrogen (N) is the main nutrient affecting the yield of the grapevine and its must and wine quality. However, little information exists about the optimum nitrate (NO3−)-N levels for vineyard soils in semiarid climatic conditions. We selected 14 vineyards of the cultivar Tempranillo (Vitis viniferaL.), with ages ranging from 10 to 25 years and located in the lower Najerilla River area (Rioja AOC). There were no climatic variations within the study area. Soil was sampled at depths of 0–15 and 15–45 cm at vineyard bloom (29 June 2010) when the grapevines peaked in their N demand, and the extractable NO3−-N in soil was determined. Nitrogen contents in both blade and petiole tissues were determined at veraison (23 August 2010). Anthocyanins and polyphenols content in the must were determined just before harvest. Finally, yield parameters such as grape weight per vine and shoot weight per vine were determined. The soil NO3−-N was significantly correlated with the N content in blade and petiole tissues. Also the soil NO3−-N contents had a significant correlation with yield parameters. Regarding must, the soil NO3−-N was negatively correlated with anthocyanins and polyphenols concentrations, so it seems that a soil with high NO3−-N availability decreases must quality. Therefore in these conditions, the determination of the available soil NO3−-N at vine flowering could be an indicator of vine N nutritional status, potential yield, and must quality. These preliminary results indicate that more studies are needed to establish an adequate soil NO3−-N content to obtain must of the highest quality. [ABSTRACT FROM AUTHOR]

Little attention has been given to the utilization of permanent cover crops in vineyard agroecosystems in semi-arid Mediterranean climatic conditions to increase soil organic carbon or its effects on N availability. We evaluated the effect of permanent cover crops with resident vegetation on labile organic matter fractions and inorganic N availability with respect to conventional tillage in a semi-arid vineyard. The field experiment was conducted on Typic Haploxerept soil, located in La Rioja area (Central River Ebro Valley). Soil samples were collected during June 2009, 5 years after covers crops were established. Soil organic carbon (SOC), water-soluble carbon (WSC), potentially mineralizable nitrogen (PMN), nitrate (N-NO3 −) and ammonium (N-NH4 +) were measured. At soil depths of 0–2.5 and 2.5–5 cm the cover crops increased SOC, WSC and PMN. So the results indicated a soil quality improvement. The cover crop treatment reduced soil N-NO3 − and increased the WSC to inorganic N ratio at 0–45 cm depth. PMN and WSC increments suggest a more active microbial biomass that could promote the immobilization and recycling of N-NO3 −, and therefore might partially explain the reduction in N-NO3 − pools under cover crops. [ABSTRACT FROM AUTHOR]

Abiotic stresses, such as temperature, heat waves, water limitation, solar radiation and the increase in atmospheric CO 2 concentration, significantly influence the accumulation of secondary metabolites in grapevine berries at different developmental stages, and in vegetative organs. Transcriptional reprogramming, miRNAs, epigenetic marks and hormonal crosstalk regulate the secondary metabolism of berries, mainly the accumulation of phenylpropanoids and of volatile organic compounds (VOCs). Currently, the biological mechanisms that control the plastic response of grapevine cultivars to environmental stress or that occur during berry ripening have been extensively studied in many worlds viticultural areas, in different cultivars and in vines grown under various agronomic managements. A novel frontier in the study of these mechanisms is the involvement of miRNAs whose target transcripts encode enzymes of the flavonoid biosynthetic pathway. Some miRNA-mediated regulatory cascades, post-transcriptionally control key MYB transcription factors, showing, for example, a role in influencing the anthocyanin accumulation in response to UV-B light during berry ripening. DNA methylation profiles partially affect the berry transcriptome plasticity of different grapevine cultivars, contributing to the modulation of berry qualitative traits. Numerous hormones (such as abscisic and jasmomic acids, strigolactones, gibberellins, auxins, cytokynins and ethylene) are involved in triggering the vine response to abiotic and biotic stress factors. Through specific signaling cascades, hormones mediate the accumulation of antioxidants that contribute to the quality of the berry and that intervene in the grapevine defense processes, highlighting that the grapevine response to stressors can be similar in different grapevine organs. The expression of genes responsible for hormone biosynthesis is largely modulated by stress conditions, thus resulting in the numeourous interactions between grapevine and the surrounding environment., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Ferrandino, Pagliarani and Pérez-Álvarez.)