37 results on '"Park, Ji-Yeong"'
Search Results
2. HSPA9 reduction exacerbates symptoms and cell death in DSS-Induced inflammatory colitis
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Jang, Soyoung, Jang, Soyeon, Ko, Jiwon, Bae, Ji-Eun, Hyung, Hyejin, Park, Ji Yeong, Lim, Su-Geun, Park, Sijun, Park, Song, Yi, Junkoo, Kim, Seonggon, Kim, Myoung Ok, Cho, Dong-Hyung, and Ryoo, Zae Young
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- 2024
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3. The relationship between caregivers’ behaviors and resistant behaviors of persons living with dementia during personal care in long-term care facilities: A lag sequential analysis
- Author
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Lee, Kyung Hee, Lee, Ji Yeon, Park, Ji Yeong, and McConnell, Eleanor S.
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- 2024
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4. Evaluation of subchronic oral dose toxicity and allergen of freeze-dried powder of Locustamigratoria (Orthoptera: Acrididae) as a novel food source
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Kim, Sun Young, Kwak, Kyu-Won, Park, Ji Yeong, Park, Eun-Sung, Nam, Chun-Ja, An, Kyu Sup, Kim, Hyun-Jin, Yoon, Hyung Joo, Kim, Yong-Soon, Park, Kwanho, Kim, Eunsun, Ryu, Hyeon Yeol, and Kim, Sun-Don
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- 2023
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5. Enhanced Drug Delivery with Oil‐in‐Water Nanoemulsions: Stability and Sustained Release of Doxorubicin.
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Hwang, Juyoung, Park, Ji Yeong, Kang, Jio, Oh, Nuri, Li, Chen, Yoo, Chung‐Yul, Um, Wooram, and Kwak, Minseok
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DRUG delivery systems , *DRUG carriers , *CARDIOTOXICITY , *TREATMENT effectiveness , *CANCER treatment , *DOXORUBICIN - Abstract
In this study, oil‐in‐water nanoemulsions are prepared, an isotropic mixture of oil, surfactant, and cosurfactants. The nanoemulsions exhibit stable structures and are capable of efficiently encapsulating hydrophobic drugs such as doxorubicin (Dox). Compared to polymeric micelles, nanoemulsions demonstrate enhanced stability and loading capacity for Dox. Furthermore, nanoemulsions release Dox steadily over 14 days, with 51.6% released within the initial 24 h and up to 80% over the subsequent period. These properties suggest that nanoemulsions can mitigate the side effects related to the burst release of Dox, thereby improving therapeutic efficacy and safety. Additionally, nanoemulsion‐treated cardiomyocytes show increased viability compared to those treated with free Dox, indicating the potential of nanoemulsions to alleviate Dox‐induced cardiotoxicity. Overall, nanoemulsions hold promise as versatile and efficient drug carriers for improving cancer treatment outcomes. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
- View/download PDF
6. Synthesis of improved long-chain isomaltooligosaccharide, using a novel glucosyltransferase derived from Thermoanaerobacter thermocopriae, with maltodextrin
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Park, Bo-Ram, Park, Ji Yeong, Lee, So Hee, Hong, Seong-Jin, Jeong, Ji Hye, Choi, Ji-Ho, Park, Shin-Yong, Park, Chan Soon, Lee, Ha-Nul, and Kim, Young-Min
- Published
- 2021
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7. Ethanol Extract of Ampelopsis brevipedunculata Rhizomes Suppresses IgE-Mediated Mast Cell Activation and Anaphylaxis.
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Park, Ji-Yeong, Kim, Min-Jong, Choi, Young-Ae, Lee, Seung Woong, Lee, Soyoung, Jang, Yong Hyun, and Kim, Sang-Hyun
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MAST cells , *ANAPHYLAXIS , *ORAL drug administration , *ATOPIC dermatitis , *ALLERGIES , *ETHANOL , *TUMOR necrosis factors , *INTRACELLULAR calcium - Abstract
More than 20% of the world's population suffers from allergic diseases, including allergic asthma, rhinitis, and atopic dermatitis that severely reduce the patient's quality of life. The treatment of allergy has been developed, but there are still unmet needs. Ampelopsis brevipedunculata (Maxim.) Trautv. is a traditional medicinal herb with beneficial bioactivities, such as antioxidant, anti-hypertension, anti-viral, anti-mutagenic, and skin and liver (anti-hepatotoxic) protective actions. However, its anti-allergic effect has not been addressed. This study designed to investigate the pharmacological effect of an ethanol extract of A. brevipedunculata rhizomes (ABE) on mast cell and anaphylaxis models. For in vivo studies, we used ovalbumin-induced active systemic anaphylaxis (ASA) and immunoglobulin (Ig) E-mediated passive cutaneous anaphylaxis (PCA) models. In ASA model, oral administration of ABE (1, 10, and 100 mg/kg) attenuated the anaphylactic responses, such as hypothermia, serum histamine, and IgE productions. In PCA model, ABE also suppressed the plasma extravasation and swelling. The underlying mechanisms of action were identified in various mast cell types. In vitro, ABE (10, 30, and 60 µg/mL) inhibited the release of essential allergic mediators, such as histamine and β-hexosaminidase, in a concentration-dependent manner. ABE prevented the rapid increase in intracellular calcium levels induced by the DNP-HSA challenge. In addition, ABE downregulated the tumor necrosis factor-α and interleukin-4 by suppressing the activation of nuclear factor-κB. Collectively, this study is the first to identify the anti-allergic effect of ABE, suggesting that ABE is a promising candidate for treating allergic diseases. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Quality changes in perilla seed powder related to storage duration and temperature
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Son, Yejin, Lee, Kyo-Yeon, Gu, Suyeon, Park, Ji Yeong, Choi, Sung-Gil, and Kim, Hyun-Jin
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- 2020
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9. Anti-Inflammatory Effects of Clematis terniflora Leaf on Lipopolysaccharide-Induced Acute Lung Injury.
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Park, Ji-Yeong, Kim, Min-Jong, Choi, Young-Ae, Kim, Yeon-Yong, Lee, Soyoung, Chung, Jae-Min, Kim, Sang-Yong, Jeong, Gil-Saeng, and Kim, Sang-Hyun
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LUNG injuries , *LIPOPOLYSACCHARIDES , *CYCLOOXYGENASE 2 , *INTERLEUKINS , *STAINS & staining (Microscopy) , *ANTI-inflammatory agents , *WESTERN immunoblotting , *NONSTEROIDAL anti-inflammatory agents , *ONE-way analysis of variance , *NF-kappa B , *MACROPHAGES , *LEAVES , *ENZYME-linked immunosorbent assay , *TUMOR necrosis factors , *DESCRIPTIVE statistics , *RESEARCH funding , *PLANT extracts , *MOLECULAR structure , *NITRIC oxide , *MITOGEN-activated protein kinases , *DATA analysis software , *ACUTE diseases , *PHARMACODYNAMICS - Abstract
For centuries, natural products are regarded as vital medicines for human survival. Clematis terniflora var. mandshurica (Rupr.) Ohwi is an ingredient of the herbal medicine, Wei Ling Xian, which has been used in Chinese medicine to alleviate pain, fever, and inflammation. In particular, C. terniflora leaves have been used to cure various inflammatory diseases, including tonsillitis, cholelithiasis, and conjunctivitis. Based on these properties, this study aimed to scientifically investigate the anti-inflammatory effect of an ethanol extract of leaves of C. terniflora (EELCT) using activated macrophages that play central roles in inflammatory response. In this study, EELCT inhibited the essential inflammatory mediators, such as nitric oxide, cyclooxygenase-2, tumor necrosis factor-α, interleukin- (IL-) 6, IL-1β, and inducible nitric oxide synthase, by suppressing the nuclear factor-κB and mitogen-activated protein kinase activation in macrophages. Acute lung injury (ALI) is a fatal respiratory disease accompanied by serious inflammation. With high mortality rate, the disease has no effective treatments. Therefore, new therapeutic agents must be developed for ALI. We expected that EELCT can be a promising therapeutic agent for ALI by reducing inflammatory responses and evaluated its action in a lipopolysaccharide- (LPS-) induced ALI model. EELCT alleviated histological changes, immune cell infiltration, inflammatory mediator production, and protein-rich pulmonary edema during ALI. Collectively, our results may explain the traditional usage of C. terniflora in inflammatory diseases and suggest the promising potential of EELCT as therapeutic candidate for ALI. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Characterization of the longitudinal bow during flexible roll forming of steel sheets
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Woo, Young Yun, Han, Sang Wook, Hwang, Tae Woo, Park, Ji Yeong, and Moon, Young Hoon
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- 2018
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11. Probiotic properties of Pediococcus strains isolated from jeotgals, salted and fermented Korean sea-food
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Lee, Kang Wook, Park, Ji Yeong, Sa, Hyun Deok, Jeong, Ji Hee, Jin, Dong Eun, Heo, Ho Jin, and Kim, Jeong Hwan
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- 2014
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12. An Evaluation of Greenness of major Korean ports: A Fuzzy Set Approach
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Park, Ji-yeong and Yeo, Gi-tae
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- 2012
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13. Probiotic properties of Weissella strains isolated from human faeces
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Lee, Kang Wook, Park, Ji Yeong, Jeong, Hee Rok, Heo, Ho Jin, Han, Nam Soo, and Kim, Jeong Hwan
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- 2012
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14. Characterization of a glutamate decarboxylase (GAD) gene from Lactobacillus zymae
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Park, Ji Yeong, Jeong, Seon-Ju, and Kim, Jeong Hwan
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- 2014
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15. Identification and characterization of Metarhizium majus isolated from the edible insect Protaetia brevitarsis in Korea.
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Kwak, Kyu‐Won, Aktaruzzaman, Md., Kim, Eunsun, Kim, Sun Young, Hong, Seung‐Beom, Park, Ji Yeong, Park, Kwanho, Koo, Bonwoo, and Kim, Yong‐Soon
- Subjects
EDIBLE insects ,METARHIZIUM ,ANTIFUNGAL agents ,AGENCY (Law) ,PREVENTIVE medicine - Abstract
Edible insects are considered novel protein sources, and the edible insect breeding industry is growing rapidly. However, fungal pathogens are a major threat to this industry, and appropriate identification of causative agents is important for the development of antifungal agents. In 2019, strains of the fungal pathogen Metarhizium were isolated from infected second and third instar larvae of the economically important edible insect Protaetia brevitarsis, which were collected from separate farms in Gimhae‐si, Gyeongsangnam‐do, Korea. Based on morphological characteristics and multilocus phylogenetic analysis of the internal transcribed spacer region, and 5′‐region of gene encoding the 1‐α elongation factor, the fungal strains were identified as Metarhizium majus. Here, we report the detailed phylogenetic characteristics and morphological description of this species, which is prevalent in Korea. Identification of the principal disease‐causing agent can help develop disease prevention and antifungal strategies for application in edible insect‐rearing farms. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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16. Overexpression of Lin28a Aggravates Psoriasis-Like Phenotype by Regulating the Proliferation and Differentiation of Keratinocytes.
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Jang, Soyeon, Jang, Soyoung, Kim, Si-Yong, Ko, Jiwon, Kim, Eungyung, Park, Ji Yeong, Hyung, Hyejin, Lee, Jin Hong, Lim, Su-Geun, Park, Sijun, Yi, Junkoo, Lee, Heon-Jin, Kim, Myoung Ok, Lee, Hyun-Shik, and Ryoo, Zae Young
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KERATINOCYTE differentiation ,BULLOUS pemphigoid ,PHENOTYPES ,SKIN diseases ,KERATINOCYTES ,CYTOKINES - Abstract
Purpose: Psoriasis is a common and well-studied autoimmune skin disease, which is characterized by plaques. The formation of psoriasis plaques occurs through the hyperproliferation and abnormal differentiation of keratinocytes, infiltration of numerous immune cells into the dermis, increased subepidermal angiogenesis, and various autoimmune-associated cytokines and chemokines. According to previous research, Lin28 regulates the let-7 family, and let-7b is associated with psoriasis. However, the link between Lin28 and psoriasis is unclear. In this study, an association was identified between Lin28a and psoriasis progression, which promoted the pathological characteristic of psoriasis in epidermal keratinocytes. Patients and Methods: This study aims to investigate the role of Lin28a and its underlying mechanism in psoriasis through in vivo and in vitro models, which include the Lin28a-overexpressing transgenic (TG) mice and Lin28a-overexpressing human keratinocyte (HaCaT) cell lines, respectively. Results: In vivo and in vitro results revealed that overexpression of Lin28a downregulated microRNA let-7 expression levels and caused hyperproliferation and abnormal differentiation in keratinocytes. In imiquimod (IMQ)-induced psoriasis-like inflammation, Lin28a overexpressing transgenic (TG) mice exhibited more severe symptoms of psoriasis. Conclusion: Mechanistically, Lin28a exacerbated psoriasis-like inflammation through the activation of the extracellular-signal-regulated kinase (ERK) and signal transducer and activator of transcription 3 signaling (STAT 3) by targeting proinflammatory cytokine interleukin-6 (IL-6). [ABSTRACT FROM AUTHOR]
- Published
- 2021
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17. Effects of different drying methods on physicochemical properties, volatile profile, and sensory characteristics of kimchi powder.
- Author
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Park, Ji Yeong, Yang, Ji-Hee, Lee, Mi-Ai, Jeong, SuYeon, and Yoo, SeungRan
- Abstract
The effects of five different drying conditions on kimchi powder quality were determined by comparatively analyzing their physicochemical characteristics, volatile profile, and sensory evaluations. The moisture content of the kimchi powder obtained by each method was < 10%, and the yield after drying differed among methods ranging from 9.50 to 10.38% (p < 0.05). Electronic nose and tongue analyses demonstrated significant differences (p < 0.05) between samples based on the drying temperature. The particle size distribution did not differ considerably between drying methods, except for the ground kimchi (p < 0.05). The sensory evaluation test revealed that the flavor and taste were rated the highest for the kimchi powder prepared using HADHT. Therefore, hot-air drying at a high temperature was the most effective method for kimchi powder production owing to have a good flavor and taste and the shorter drying time. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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18. Effect of pasteurization on delayed kimchi ripening and regression analysis for shelf life estimation of kimchi.
- Author
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Lee, Hyun‐Gyu, Jeong, Suyeon, Park, Ji Yeong, and Yoo, SeungRan
- Subjects
KIMCHI ,FOOD pasteurization ,SHELF-life dating of food ,REGRESSION analysis ,LACTIC acid bacteria - Abstract
Pasteurization‐mediated delayed kimchi ripening and regression analysis for shelf life estimation were investigated. Various initial kimchi microbial communities were simplified to lactic acid bacteria Leuconostoc sp. and Lactobacillus sp. over time, with concomitant pH decrease from 6.39 to 4.34 and acidity increase from 0.06% to 0.35%. Other quality characteristics (organic acid, carbon dioxide, and microbial population) also changed, exhibiting high intercorrelation. Pasteurization decreased the initial bacterial counts from 5.20 to 1.92 log CFU/g, thereby delaying the change in quality characteristics (pH, acidity, organic acid, microbial population, carbon dioxide, and microbial community); however, the texture did not differ significantly (p < 0.05). In addition, the regression equation for the relationship between acidity and carbon dioxide levels suggested that shelf life could be estimated in conjunction with the ideal gas equation. In conclusion, pasteurization and regression analysis for kimchi shelf life estimation may enable the maintenance of quality and effective management during the distribution process. We characterized changes in quality characteristics and microbial community during kimchi fermentation and the effects of pasteurization on lactic acid bacteria growth. Experimental data were utilized to establish regression equations to determine acidity as a measure of carbon dioxide accumulation, which were used for shelf life estimation, thus facilitating effective management of packaging volume expansion and breakage as well as quality maintenance during distribution. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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19. Construction of a shuttle vector based on the small cryptic plasmid pJY33 from Weissella cibaria 33.
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Park, Ji Yeong, Jeong, Seon-Ju, Sa, Hyun Deok, Lee, Jae Yong, Liu, Xiaoming, Cho, Min Jeong, Lee, Kang Wook, and Kim, Jeong Hwan
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PLASMIDS , *OPEN reading frames (Genetics) , *GENETIC code , *ESCHERICHIA coli , *LEUCONOSTOC mesenteroides , *GALACTOSIDASES - Abstract
A cryptic plasmid, pJY33, from Weissella cibaria 33 was characterized. pJY33 was 2365 bp in size with a GC content of 41.27% and contained two putative open reading frames (ORFs). orf1 encoded a putative hypothetical protein of 134 amino acids. orf2 was 849 bp in size, and its putative translation product exhibited 87% identity with a replication initiation factor from a plasmid from W. cibaria KLC140. A Weissella - Escherichia coli shuttle vector, pJY33E (6.5 kb, Em r ), was constructed by ligation of pJY33 with pBluescript II SK(−) and an erythromycin resistance gene (Em r ). pJY33E replicated in Lactococcus lactis , Leuconostoc citreum , Lactobacillus brevis, Lactobacillus plantarum, and Weissella confusa . A single-stranded DNA intermediate was detected from Lb. brevis 2.14 harbouring pJY33E, providing evidence for rolling-circle replication of pJY33. Most Lb. brevis 2.14 cells (85.9%) retained pJY33E after one week of daily culturing in MRS broth without Em. An aga gene encoding α-galactosidase (α-Gal) from Leuconostoc mesenteroides was successfully expressed in Lb. brevis 2.14 using pJY33E, and the highest level of α-Gal activity (36.13 U/mg protein) was observed when cells were grown on melibiose. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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20. Genomics-based discovery of macrolide glycosyltransferase from Bacillus sp.
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Kim, Won-Gon, Park, Ji Yeong, and Kim, Hyun Ju
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- 2014
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21. Ginsenosides Protect Apical Transporters of Cultured Proximal Tubule Cells from Dysfunctions Induced by H2O2.
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Han, Ho Jae, Yoon, Byung Cheol, Park, Soo Hyun, Park, Ji Yeong, Oh, Young Joon, Lee, Yun Jung, and Park, Kwon Moo
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- 2002
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22. Ginsenosides Inhibit EGF-Induced Proliferation of Renal Proximal Tubule Cells via Decrease of c-fos and c-jun Gene Expression in vitro.
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Han, Ho Jae, Yoon, Byeong Cheol, Lee, Sang Hern, Park, Soo Hyun, Park, Ji Yeong, Oh, Young Joon, and Lee, Yun Jung
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- 2002
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23. Genomics-based discovery of macrolide glycosyltransferase from Bacillus sp.
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Won-Gon Kim, Park, Ji Yeong, and Hyun Ju Kim
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- 2014
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24. Subcellular localization of WD40 repeat 1 protein in PC12 rat pheochromocytoma cells
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Shin, Dong Hoon, Lee, Eunju, Chung, Yoon Hee, Mun, Ga Hee, Park, Ji yeong, Lomax, Margaret I., and Oh, Seung Ha
- Subjects
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PROTEINS , *PHEOCHROMOCYTOMA , *PEPTIDES , *IMMUNOCYTOCHEMISTRY , *ELECTRON microscopes - Abstract
The dynamics of actin filament protein is crucial for various physiological processes of the cells. Among the proteins correlating with actin dynamics, a novel 67-kDa WD40 repeat protein 1 (WDR1) was the vertebrate homologue of actin-interacting protein 1 (Aip1). Even though previous studies have provided the clues on the function of WDR1 in specific organs under pathological conditions, the exact subcellular localization of WDR1 is not known. Therefore, in the present study, we undertook to determine the distribution of WDR1 within PC12 pheochromocytoma cells (PC12 cells) using light and electron microscopic techniques.Double immunocytochemistry clearly showed that WDR1 immunoreactivities (IRs) were co-localized with anti-actin antibody, suggesting the involvement of WDR1 in actin dynamics. WDR1 immunoreactivities (IRs) in PC12 cells showed different distribution patterns as nerve growth factor (NGF) concentrations varied. During active proliferation, the distribution of WDR1 IRs seemed to be similar to those found in cortical actin patches, whereas WDR1 IR was observed in cytoplasmic actin cables after PC12 cells were induced to differentiate by treating with NGF. Though further studies are necessary to determine the function of WDR1, the current data represents a first step towards the in vitro study of WDR1 protein. [Copyright &y& Elsevier]
- Published
- 2004
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25. Effects of different drying methods on physicochemical properties, volatile profile, and sensory characteristics of kimchi powder.
- Author
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Park JY, Yang JH, Lee MA, Jeong S, and Yoo S
- Abstract
The effects of five different drying conditions on kimchi powder quality were determined by comparatively analyzing their physicochemical characteristics, volatile profile, and sensory evaluations. The moisture content of the kimchi powder obtained by each method was < 10%, and the yield after drying differed among methods ranging from 9.50 to 10.38% ( p < 0.05). Electronic nose and tongue analyses demonstrated significant differences ( p < 0.05) between samples based on the drying temperature. The particle size distribution did not differ considerably between drying methods, except for the ground kimchi ( p < 0.05). The sensory evaluation test revealed that the flavor and taste were rated the highest for the kimchi powder prepared using HADHT. Therefore, hot-air drying at a high temperature was the most effective method for kimchi powder production owing to have a good flavor and taste and the shorter drying time.
- Published
- 2018
- Full Text
- View/download PDF
26. Next Generation Proteomic Pipeline for Chromosome-Based Proteomic Research Using NeXtProt and GENCODE Databases.
- Author
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Hwang H, Park GW, Park JY, Lee HK, Lee JY, Jeong JE, Park SR, Yates JR 3rd, Kwon KH, Park YM, Lee HJ, Paik YK, Kim JY, and Yoo JS
- Subjects
- Alternative Splicing, Hippocampus chemistry, Humans, Male, Protein Processing, Post-Translational, Spermatozoa chemistry, Testis chemistry, Brain Chemistry, Chromosomes, Human genetics, Databases, Protein, Proteomics methods
- Abstract
Human Proteome Project aims to map all human proteins including missing proteins as well as proteoforms with post translational modifications, alternative splicing variants (ASVs), and single amino acid variants (SAAVs). neXtProt and Ensemble databases are usually used to provide curated information on human coding genes. However, to find these proteoforms, we (Chr #11 team) first introduce a streamlined pipeline using customized and concatenated neXtProt and GENCODE originated from Ensemble, with controlled false discovery rate (FDR). Because of large sized databases used in this pipeline, we found more stringent FDR filtering (0.1% at the peptide level and 1% at the protein level) to claim novel findings, such as GENCODE ASVs and missing proteins, from human hippocampus data set (MSV000081385) and ProteomeXchange (PXD007166). Using our next generation proteomic pipeline (nextPP) with neXtProt and GENCODE databases, two missing proteins such as activity-regulated cytoskeleton-associated protein (ARC, Chr 8) and glutamate receptor ionotropic, kainite 5 (GRIK5, Chr 19) were additionally identified with two or more unique peptides from human brain tissues. Additionally, by applying the pipeline to human brain related data sets such as cortex (PXD000067 and PXD000561), spinal cord, and fetal brain (PXD000561), seven GENCODE ASVs such as ACTN4-012 (Chr.19), DPYSL2-005 (Chr.8), MPRIP-003 (Chr.17), NCAM1-013 (Chr.11), EPB41L1-017 (Chr.20), AGAP1-004 (Chr.2), and CPNE5-005 (Chr.6) were identified from two or more data sets. The identified peptides of GENCODE ASVs were mapped onto novel exon insertions, alternative translations at 5'-untranslated region, or novel protein coding sequence. Applying the pipeline to male reproductive organ related data sets, 52 GENCODE ASVs were identified from two testis (PXD000561 and PXD002179) and a spermatozoa (PXD003947) data sets. Four out of 52 GENCODE ASVs such as RAB11FIP5-008 (Chr. 2), RP13-347D8.7-001 (Chr. X), PRDX4-002 (Chr. X), and RP11-666A8.13-001 (Chr. 17) were identified in all of the three samples.
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- 2017
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27. Isolation and Characterization of Some Promoter Sequences from Leuconostoc mesenteroides SY2 Isolated from Kimchi.
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Park JY, Jeong SJ, Kim JA, and Kim JH
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- DNA, Bacterial analysis, DNA, Bacterial genetics, Escherichia coli genetics, Escherichia coli metabolism, Genetic Vectors genetics, Humans, Leuconostoc mesenteroides isolation & purification, Republic of Korea, alpha-Amylases genetics, alpha-Amylases metabolism, Cloning, Molecular methods, Fermented Foods microbiology, Leuconostoc mesenteroides genetics, Promoter Regions, Genetic genetics
- Abstract
Some promoters were isolated and characterized from the genome of Leuconostoc mesenteroides SY2, an isolate from kimchi, a Korean traditional fermented vegetable. Chromosomal DNA of L. mesenteroides SY2 was digested with Sau3AI and ligated with BamHI-cut pBV5030, a promoter screening vector containing a promoterless cat-86 . Among E. coli transformants (TFs) resistant against Cm (chloramphenicol), 17 were able to grow in the presence of 1,000 μg/ml Cm and their inserts were sequenced. Transcription start sites were examined for three putative promoters (P04C, P25C, and P33C) by primer extension. Four putative promoters were inserted upstream of a promoterless α-amylase reporter gene in pJY15α. α-Amylase activities of E. coli TFs containing pJY15α (control, no promoter), pJY03α (pJY15α with P03C), pJY04α (with P04C), pJY25α (with P25C), and pJY33α (with P33C) were 66.9, 78.7, 122.1, 70.8, and 99.3 U, respectively. Cells harboring pJY04α showed 1.8 times higher activity than the control. Some promoters characterized in this study might be useful for construction of foodgrade expression vectors for Leuconostoc sp. and related lactic acid bacteria.
- Published
- 2017
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28. Integrated Proteomic Pipeline Using Multiple Search Engines for a Proteogenomic Study with a Controlled Protein False Discovery Rate.
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Park GW, Hwang H, Kim KH, Lee JY, Lee HK, Park JY, Ji ES, Park SR, Yates JR 3rd, Kwon KH, Park YM, Lee HJ, Paik YK, Kim JY, and Yoo JS
- Subjects
- Alternative Splicing, Computational Biology methods, Databases, Protein, False Positive Reactions, Humans, Mass Spectrometry methods, Hippocampus chemistry, Proteogenomics methods, Proteomics methods, Search Engine
- Abstract
In the Chromosome-Centric Human Proteome Project (C-HPP), false-positive identification by peptide spectrum matches (PSMs) after database searches is a major issue for proteogenomic studies using liquid-chromatography and mass-spectrometry-based large proteomic profiling. Here we developed a simple strategy for protein identification, with a controlled false discovery rate (FDR) at the protein level, using an integrated proteomic pipeline (IPP) that consists of four engrailed steps as follows. First, using three different search engines, SEQUEST, MASCOT, and MS-GF+, individual proteomic searches were performed against the neXtProt database. Second, the search results from the PSMs were combined using statistical evaluation tools including DTASelect and Percolator. Third, the peptide search scores were converted into E-scores normalized using an in-house program. Last, ProteinInferencer was used to filter the proteins containing two or more peptides with a controlled FDR of 1.0% at the protein level. Finally, we compared the performance of the IPP to a conventional proteomic pipeline (CPP) for protein identification using a controlled FDR of <1% at the protein level. Using the IPP, a total of 5756 proteins (vs 4453 using the CPP) including 477 alternative splicing variants (vs 182 using the CPP) were identified from human hippocampal tissue. In addition, a total of 10 missing proteins (vs 7 using the CPP) were identified with two or more unique peptides, and their tryptic peptides were validated using MS/MS spectral pattern from a repository database or their corresponding synthetic peptides. This study shows that the IPP effectively improved the identification of proteins, including alternative splicing variants and missing proteins, in human hippocampal tissues for the C-HPP. All RAW files used in this study were deposited in ProteomeXchange (PXD000395).
- Published
- 2016
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29. Improvement of Fibrinolytic Activity of Bacillus subtilis 168 by Integration of a Fibrinolytic Gene into the Chromosome.
- Author
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Jeong SJ, Park JY, Lee JY, Lee KW, Cho KM, Kim GM, Shin JH, Kim JS, and Kim JH
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- Bacillus subtilis growth & development, Bacillus subtilis metabolism, Culture Media chemistry, Recombinant Proteins genetics, Recombinant Proteins metabolism, Bacillus subtilis enzymology, Bacillus subtilis genetics, Chromosomes, Bacterial, Fibrinolysin genetics, Fibrinolysin metabolism, Fibrinolysis, Genes, Bacterial
- Abstract
Fibrinolytic enzyme genes (aprE2, aprE176, and aprE179) were introduced into the Bacillus subtilis 168 chromosome without any antibiotic resistance gene. An integration vector, pDG1662, was used to deliver the genes into the amyE site of B. subtilis 168. Integrants, SJ3-5nc, SJ176nc, and SJ179nc, were obtained after two successive homologous recombinations. The integration of each fibrinolytic gene into the middle of the amyE site was confirmed by phenotypes (Amy(-), Spec(S)) and colony PCR results for these strains. The fibrinolytic activities of the integrants were higher than that of B. subtilis 168 by at least 3.2-fold when grown in LB broth. Cheonggukjang was prepared by inoculating each of B. subtilis 168, SJ3-5nc, SJ176nc, and SJ179nc, and the fibrinolytic activity of cheonggukjang was 4.6 ± 0.7, 10.8 ± 0.9, 7.0 ± 0.6, and 8.0 ± 0.2 (U/g of cheonggukjang), respectively at 72 h. These results showed that construction of B. subtilis strains with enhanced fibrinolytic activities is possible by integration of a strong fibrinolytic gene via a marker-free manner.
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- 2015
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30. Characterization of Glutamate Decarboxylase (GAD) from Lactobacillus sakei A156 Isolated from Jeot-gal.
- Author
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Sa HD, Park JY, Jeong SJ, Lee KW, and Kim JH
- Subjects
- Amino Acid Sequence, Bacterial Proteins genetics, Bacterial Proteins isolation & purification, Escherichia coli genetics, Fermentation, Glutamate Decarboxylase genetics, Glutamate Decarboxylase isolation & purification, Lactobacillus genetics, Molecular Sequence Annotation, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Republic of Korea, Sequence Alignment, gamma-Aminobutyric Acid metabolism, Bacterial Proteins chemistry, Bacterial Proteins metabolism, Glutamate Decarboxylase chemistry, Glutamate Decarboxylase metabolism, Lactobacillus enzymology, Seafood microbiology
- Abstract
A gamma-aminobutyric acid (GABA)-producing microorganism was isolated from jeot-gal (anchovy), a Korean fermented seafood. The isolate, A156, produced GABA profusely when incubated in MRS broth with monosodium glutamate (3% (w/v)) at 37°C for 48 h. A156 was identified as Lactobacillus sakei by 16S rRNA gene sequencing. The GABA conversion yield was 86% as determined by GABase enzyme assay. The gadB gene encoding glutamate decarboxylase (GAD) was cloned by PCR. gadC encoding a glutamate/GABA antiporter was located immediately upstream of gadB. The operon structure of gadCB was confirmed by RT-PCR. gadB was overexpressed in Escherichia coli BL21(DE3) and recombinant GAD was purified. The purified GAD was 54.4 kDa in size by SDS-PAGE. Maximum GAD activity was observed at pH 5.0 and 55°C and the activity was dependent on pyridoxal 5'-phosphate. The Km and Vmax of GAD were 0.045 mM and 0.011 mM/min, respectively, when glutamate was used as the substrate.
- Published
- 2015
- Full Text
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31. Characterization of AprE176, a fibrinolytic enzyme from Bacillus subtilis HK176.
- Author
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Jeong SJ, Heo K, Park JY, Lee KW, Park JY, Joo SH, and Kim JH
- Subjects
- Amino Acid Sequence, Amino Acid Substitution, Animals, Bacillus subtilis genetics, Bacillus subtilis isolation & purification, Base Sequence, Binding Sites, Cloning, Molecular, Enzyme Stability, Escherichia coli genetics, Fermentation, Fibrin metabolism, Fibrinolysin metabolism, Milk, Models, Molecular, Molecular Sequence Data, Mutation, Polymerase Chain Reaction, Sequence Alignment, Serine Proteases chemistry, Serine Proteases genetics, Serine Proteases isolation & purification, Temperature, Bacillus subtilis enzymology, Fibrinogen metabolism, Fibrinolysis genetics, Serine Proteases metabolism, Soy Foods microbiology
- Abstract
Bacillus subtilis HK176 with high fibrinolytic activity was isolated from cheonggukjang, a Korean fermented soyfood. A gene, aprE176, encoding the major fibrinolytic enzyme was cloned from B. subtilis HK176 and overexpressed in E. coli BL21(DE3) using plasmid pET26b(+). The specific activity of purified AprE176 was 216.8 ± 5.4 plasmin unit/mg protein and the optimum pH and temperature were pH 8.0 and 40°C, respectively. Error-prone PCR was performed for aprE176, and the PCR products were introduced into E. coli BL21(DE3) after ligation with pET26b(+). Mutants showing enhanced fibrinolytic activities were screened first using skim-milk plates and then fibrin plates. Among the mutants, M179 showed the highest activity on a fibrin plate and it had one amino acid substitution (A176T). The specific activity of M179 was 2.2-fold higher than that of the wild-type enzyme, but the catalytic efficiency (kcat/Km) of M179 was not different from the wild-type enzyme owing to reduced substrate affinity. Interestingly, M179 showed increased thermostability. M179 retained 36% of activity after 5 h at 45°C, whereas AprE176 retained only 11%. Molecular modeling analysis suggested that the 176(th) residue of M179, threonine, was located near the cation-binding site compared with the wild type. This probably caused tight binding of M179 with Ca(2+), which increased the thermostability of M179.
- Published
- 2015
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32. Proteomic analysis of proteins increased or reduced by ethanol of Lactobacillus plantarum ST4 isolated from Makgeolli, traditional Korean rice wine.
- Author
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Lee SG, Lee KW, Park TH, Park JY, Han NS, and Kim JH
- Subjects
- Bacterial Proteins analysis, Bacterial Proteins genetics, Fermentation, Gene Expression Regulation, Bacterial, Lactobacillus plantarum chemistry, Lactobacillus plantarum genetics, Molecular Sequence Data, Oryza metabolism, Wine analysis, Bacterial Proteins metabolism, Ethanol metabolism, Lactobacillus plantarum isolation & purification, Lactobacillus plantarum metabolism, Oryza microbiology, Proteomics, Wine microbiology
- Abstract
LAB were isolated from makgeolli locally produced around Jinju, Gyeongnam, S. Korea during spring of 2011. Randomly selected 11 isolates from MRS agar plates were identified first by API CHL 50 kits and then 16S rRNA gene sequencing. All 11 isolates were identified as Lactobacillus plantarum. Among them, ST4 grew in MRS broth with ethanol up to 10%, showing the highest alcohol resistance. L. plantarum ST4 was moderately resistant against acid and bile salts. When cellular proteins of L. plantarum ST4 under ethanol stress were analyzed by two-dimensional gel electrophoresis (2DE), the intensities of 6 spots increased, whereas 22 spots decreased at least 2-fold. Those 28 spots were identified by peptide mass fingerprinting (PMF). FusA2 (elongation factor G) increased 18.8-fold (6% ethanol) compared with control. Other proteins were AtpD (ATP synthase subunit beta), DnaK, GroEL, Tuf (elongation factor Tu), and Npr2 (NADH peroxidase), respectively. Among the 22 proteins decreased in intensities, lactate dehydrogenases (LdhD and LdhL1) were included.
- Published
- 2012
- Full Text
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33. Expression of alpha-galactosidase gene from Leuconostoc mesenteroides SY1 in Leuconostoc citreum.
- Author
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Park JY, Jeong SJ, Lee AR, Park JY, Jeong WJ, and Kim JH
- Subjects
- Blotting, Western, DNA, Bacterial chemistry, DNA, Bacterial genetics, Electroporation, Gene Expression, Leuconostoc genetics, Polymerase Chain Reaction, Transcription, Genetic genetics, Transcription, Genetic physiology, alpha-Galactosidase genetics, Leuconostoc enzymology, alpha-Galactosidase biosynthesis
- Abstract
A 2.5 kb aga gene encoding alpha-galactosidase (alpha- Gal) from Leuconostoc mesenteroides SY1 was cloned into pSJE, an E. coli-Leuconostoc shuttle vector. The recombinant plasmid, pSJEaga, was introduced into Leuconostoc citreum KCTC3526 (ATCC49370) by electroporation. Transcription level of aga was the highest in cells grown on raffinose (1%, w/v) followed by cells grown on galactose, melibiose, fructose, glucose, and sucrose. Western blot using antibodies against alpha-Gal showed similar results to slot-blot results and enzyme activity measurements. All the results indicated that the heterologous aga was successfully expressed in L. citreum and its transcription was under the carbon catabolite repression (CCR).
- Published
- 2007
34. Insulin-like growth factor binding protein (IGFBP)-mediated hair cell survival on the mouse utricle exposed to neomycin: the roles of IGFBP-4 and IGFBP-5.
- Author
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Park JY, Park YH, Shin DH, and Oh SH
- Subjects
- Animals, Apoptosis drug effects, Cell Survival drug effects, Cell Survival physiology, Cells, Cultured, Hair Cells, Auditory metabolism, Hair Cells, Auditory pathology, Immunohistochemistry, Insulin-Like Growth Factor Binding Protein 4 metabolism, Insulin-Like Growth Factor Binding Protein 5 metabolism, Insulin-Like Growth Factor I metabolism, Intracellular Space metabolism, Mice, Mice, Inbred ICR, Models, Animal, Anti-Bacterial Agents toxicity, Hair Cells, Auditory drug effects, Insulin-Like Growth Factor Binding Protein 4 pharmacology, Insulin-Like Growth Factor Binding Protein 5 pharmacology, Insulin-Like Growth Factor I pharmacology, Neomycin toxicity
- Abstract
Conclusion: This study suggests for the first time that 1) IGF-I, IGFBP-4, and -5 alone and IGF-I+IGFBP-5 mixture stimulated hair cell survival and prevented neomycin-induced hair cell loss in the sensory epithelial culture of mouse utricles, 2) When administered together, IGFBP-4 diminished the effect of IGF-I, 3) In P3-5 mice utricle, IGF-I, IGFBP-4, and IGFBP-5 are expressed in the cytoplasm of hair cells. And Insulin/IGF-I Receptor is expressed in the nucleus of hair cells., Objectives: Several growth factors have been demonstrated to protect auditory sensory cells in vitro and in vivo from aminoglycoside toxicity. IGF-I is one of the most well-known mitogenic and protective substance working in the inner ear. However, there are no reports available regarding the function of IGFBPs in the inner ear. In the present study, the effects of IGFBP-4 and -5 on hair cell survival were investigated in mouse utriclular organ cultures., Materials and Methods: The amount of cellular damage and cell viability in vestibular organs were assessed by counting hair cells stained with a rhodamine-phalloidin probe. The expressions of IGFBP-4, IGFBP-5, IGF-IR, and IGF-I were localized by immunohistochemistry., Results: When treated with IGF-I, IGFBP-4, or IGFBP-5 for 24 h, explant culture showed hair cell survival rates of 136+/-18%, 140+/-15%, and 133+/-6%, respectively, compared to controls. Neomycin (1 mM) induced hair cell loss resulted in 45+/-17% of hair cell survival. However, pre-treatment of IGF-I, IGFBP-4, or -5 before neomycin insult showed survival rates of 113+/-14%, 98+/-8%, and 73+/-24%, respectively. Similar to IGF-I, IGFBP-4 and IGFBP-5 were significantly protective. IGFBP-4 and -5 immunoreactivities were observed in the cytoplasm of normal explanted vestibular hair cells as well as in the P3 mouse utricular hair cells in vivo.
- Published
- 2007
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- View/download PDF
35. WDR1 expression in the normal and noise-damaged chick vestibule.
- Author
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Suh MW, Shin DH, Lee HS, Park JY, Kim CS, and Oh SH
- Subjects
- Acoustic Stimulation adverse effects, Animals, Chickens, Gene Expression, Immunohistochemistry, Protein Transport, RNA, Messenger metabolism, Regeneration, Reverse Transcriptase Polymerase Chain Reaction, Vestibule, Labyrinth physiology, Hearing Loss, Noise-Induced metabolism, Microfilament Proteins biosynthesis, Vestibule, Labyrinth metabolism
- Abstract
Unlike mammals, avian cochlear hair cells can regenerate after acoustic overstimulation. The WDR1 gene is one of the genes suspected to play an important role in this difference. In an earlier study, we found that the WDR1 gene is over-expressed in the chick cochlea after acoustic overstimulation. The aim of this study was to compare the expression of WDR1 before and after acoustic overstimulation in the chick vestibule. Seven-day-old chicks were divided into three groups: normal group, damage group, and regeneration group. The damage and regeneration group was exposed to 120 dB SPL white noise for 5-6 hours. The damage group was euthanized shortly after the impulse, but the regeneration group was allowed to recover for 2 days. The utricle, saccule, and the three ampullae of each semicircular canal were dissected and immunohistochemically stained with anti-WD40 repeat protein 1 antibody. For quantitative analysis, immunoreactive densities were measured and quantitative real-time RT PCR was performed. WD40 repeat protein 1 expression was elevated in all the semicircular canals and utricle, two days after an acoustic overstimulation (P=0.001). WDR1 mRNA expression was 1.34 times higher in the regeneration group compared to the normal group, but it was not statistically significant. Exceptionally, WD40 repeat protein 1 expression did not increase in the saccule of the regeneration group. Elevated WDR1 expression in the avian vestibule may have a role in the hair cell regenerating ability as in the avian cochlea. A similar mechanism of hair cell regeneration may exist in the avian cochlea and vestibule.
- Published
- 2007
36. Effect of epidermal growth factor on phosphate uptake in renal proximal tubule cells: involvement of PKC, MAPK, and cPLA2.
- Author
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Han HJ, Park JY, Lee YJ, and Park SH
- Subjects
- Animals, Arachidonic Acid metabolism, Cells, Cultured, Dose-Response Relationship, Drug, Enzyme Inhibitors pharmacology, Group IV Phospholipases A2, Kidney Tubules, Proximal drug effects, Kidney Tubules, Proximal metabolism, Kinetics, Male, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinases metabolism, Phospholipases A metabolism, Phosphorylation drug effects, Protein Kinase C metabolism, Rabbits, Epidermal Growth Factor pharmacology, Kidney Tubules, Proximal cytology, Phosphates metabolism, Signal Transduction
- Abstract
Objective: The present study was conducted to examine the effect of epidermal growth factor (EGF) on Pi uptake and its related signal pathways in the primary cultured renal proximal tubule cells (PTCs)., Results: EGF (50 ng/ml) inhibited Pi uptake, a typical marker of Na(+)/phosphate cotransporter, in a time- and dose-dependent manner. EGF-induced inhibition of Pi uptake was blocked by AG1478 (an EGF receptor antagonist), genistein or herbimycin A (tyrosine kinase inhibitors) and also blocked by mepacrine (a phospholipase A(2) (PLA(2)) inhibitor) and AACOCF(3 )(a cPLA(2) inhibitor). EGF increased [(3)H]-arachidonic acid (AA) release, which was also blocked by AG1478, genistein or herbimycin. Furthermore, EGF-induced inhibition of Pi uptake was blocked by indomethacin (a cyclooxygenase inhibitor) and econazole (a cytochrome P-450 epoxygenase inhibitor), but not by NDGA (a lipoxygenase inhibitor). On the other hand, EGF-induced inhibition of Pi uptake was blocked by staurosporine, H-7, or bisindolylmaleimide I (PKC inhibitors), PD 98059 (a p44/42 MAPK inhibitor), but not by SB 203580 (a p38 MAPK inhibitor). EGF-induced increase of [(3)H]-AA release was blocked by PKC inhibitors and a p44/42 mitogen-activated protein kinase (MAPK) inhibitor, but not by a p38 MAPK inhibitor. In addition, a PKC inhibitor blocked EGF-induced phosphorylation of p44/42 MAPK., Conclusion: EGF inhibits Pi uptake via PKC-p44/42 MAPK-cPLA(2) pathway in the PTCs., (Copyright 2003 S. Karger AG, Basel)
- Published
- 2003
- Full Text
- View/download PDF
37. Ginsenosides protect apical transporters of cultured proximal tubule cells from dysfunctions induced by h(2)o(2).
- Author
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Han HJ, Yoon BC, Park SH, Park JY, Oh YJ, Lee YJ, and Park KM
- Subjects
- Animals, Arachidonic Acid metabolism, Calcium pharmacokinetics, Dose-Response Relationship, Drug, Hydrogen Peroxide administration & dosage, Kidney Tubules, Proximal cytology, Lipid Peroxides metabolism, Male, Methylglucosides pharmacokinetics, Oxidative Stress physiology, Phosphorus pharmacokinetics, Rabbits, Sapogenins pharmacology, Sodium pharmacokinetics, Triterpenes pharmacology, Ginsenosides pharmacology, Hydrogen Peroxide pharmacology, Kidney Tubules, Proximal metabolism, Membrane Transport Proteins drug effects, Membrane Transport Proteins metabolism
- Abstract
Oxidative stress has been implicated as a primary cause of renal failure in certain renal diseases. Indeed, renal proximal tubule is a very sensitive site to oxidative stress and retains functionally fully characterized transporters. It has been reported that ginsenosides have a beneficial effect on diverse diseases including oxidative stress. However, the protective effect of ginsenosides on oxidative stress has not been elucidated in renal proximal tubule cells. Thus, we examined the effect of ginsenosides on oxidative stress-induced alteration of apical transporters and its related mechanism in renal proximal tubule cells. In the present study, hydrogen peroxide (H(2)O(2)) (>10(-5) M) inhibited alpha-methyl-D-glucopyranoside uptake in a dose-dependent manner (p < 0.05). It also inhibited Pi and Na(+) uptake. At a concentration of 20 microg/ml, total ginsenosides significantly reduced H(2)O(2)-induced inhibition of apical transporters. In contrast, protopanaxadiol (PD) and protopanaxatriol (PT) saponins exhibited a less preventive effect than total ginsenosides (p < 0.05). Furthermore, we examined its action mechanism. H(2)O(2) increased lipid peroxide formation, arachidonic acid (AA) release, and Ca(2+) uptake. These effects on H(2)O(2) were significantly prevented by total ginsenosides and PD or PT sanponins. However, total ginsenosides appear to be more protective than PD and PT saponins (p < 0.05). In conclusion, ginsenosides prevented H(2)O(2)-induced inhibition of apical transporters via a decrease in oxidative stress, AA release, and Ca(2+) uptake in primary cultured renal proximal tubule cells., (Copyright 2002 S. Karger AG, Basel)
- Published
- 2002
- Full Text
- View/download PDF
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