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Introduction/Objective. The aim of the study was to examine the progression of diabetic nephropathy (DN) in a prospective three-year period as well as to establish the risk factors for DN progression. Methods. The study involved 45 patients with type 2 diabetes and DN (26 males, aged 18–62 years) followed up for three years. All the patients underwent physical examination and laboratory analysis at each visit. Laboratory analyses included complete blood count, serum glucose, urea, creatinine, protein, lipid concentration, glycosylated hemoglobin (HbA1c) and urine protein, albumin and creatinine concentration. Glomerular filtration rate (GFR) was calculated using Modification of Diet in Renal Disease formula. Kidney length and parenchymal thickness were measured by ultrasound. Results. Fasting serum glucose concentration (12.0 ± 2.79 vs. 9.50 ± 2.22, p < 0.001) and HbA1c (7.99 ± 1.43 vs. 7.49 ± 1.29, p < 0.031) were decreased over the three years. Albuminuria increased (43.75 ± 10.83 vs. 144.44 ± 52.70 mg/l, p < 0.001) and GFR (63 vs. 58.3 ml/min/1.73 m2) decreased significantly during the study, but serum lipid concentration remained unchanged. Mean kidney length and parenchymal thickness decreased during the three years. Linear regression analysis found systolic blood pressure, fasting glycemia, HbA1c as positive and kidney length and parenchymal thickness as negative predictors of proteinuria increase, but proteinuria as negative and serum iron and albumin concentrations as positive predictors of annual change in GFR. Conclusion High blood pressure and high HbA1c are selected as significant risk factors for increasing proteinuria, which is a significant predictor of GFR decreasing in patients with DN.

Introduction: During the course and development of diverse cardiological diseases different central nervous system complications may develop. These are most frequently related to the nature of the cardiovascular entity itself. Aim: To evaluate the association between atherogenesis factors and border zones strokes within the cerebral medial artery vascularization territories. Methods: In total 30 patients were enrolled in the study. Stroke diagnosis was based according to clinical and neurological examination and brain CT findings. The study analyzed risk factors correspondingly to World Health Organization criteria. Results: In majority of patients (18; 60%) arterial hypertension was diagnosed. Additionally, following condition and risk factors were identified: cardiological diseases (17; 23.3%), cigarette smoking (13, 43.3%), hypercholeterolaemia (9; 30%) and diabetes (7; 23.3%). In the group with cardiological diseases most frequent was myocardial infarction (9; 56.2%), AV block II and III (5, 29.4%), atrial fibrillation with slow ventricular response (3; 17.6%). Conclusion: Results of the study point to conclusion that myocardial infarction, AV block II and III, atrial fibrillation with slow ventricular response, iatrogenic hypertension and traditional risk factors for atherogenesis significantly influences presentation of the border zone strokes within the cerebral medial artery vascularization territories.

The article presents a study regarding the heat transfer on run-out table (ROT) cooling of jets. The researchers observe and test samples of steel with thermocouples inside. They also examine the impacts of jet configuration on ROT cooling of steel. They conclude that multiple top jet work determines the heat transfer on plates. In addition, they learn that heat transfer depends on nozzle.

The growth in demand for high quality metal alloys has placed considerable emphasis on the type of cooling methods used in manufacturing processes, in particular, the production of highly tailored steel through controlled cooling on the runout table. The present study focuses on the heat transfer (cooling of hot rolled steel strips) on a runout table. The purpose of the study was to develop an efficient experimental method and collect temperature data under conditions similar to those that occur during industrial runout table conditions in a steelmill. Surface and internal temperatures were measured during transient cooling of a flat, upward facing fixed steel plate cooled by a highly subcooled single, circular, free surface jet of water. Measurements were made at stagnation and several streamwise distances from the stagnation point. A numerical, finite difference model was applied to calculate the surface heat flux using measured temperatures. The effect of water flowrate and subcooling on the overall heat transfer with emphasis on the maximum heat flux is discussed. [ABSTRACT FROM AUTHOR]

This paper describes the issues related to the investment securities market, and the function of the role of banks in the process. Here, the emphasis is on the experiences of developed countries, especially the U.S.A. In recent years, banks and other deponent institutions have had a tendency to accept higher risks and more complex investments in their portfolio. There have appeared new instruments whose purpose is more than just provision of liquidity and income, especially, in cases of poorloan demand. An instrument for swap of the bank portfolio and other institutions- lenders mostly practice replacing the portfolios of their investment securities,bearing in mind tax,as well as higher revenues

Introduction Alcoholic liver disease represents a growing global pandemic, particularly among younger men, and is one of the leading causes of premature death worldwide. Observing complications during the decompensation stage and monitoring disease progression dynamics using scoring systems are particularly important. Materials and methods This retrospective-prospective, descriptive, and analytical study included 123 male patients with a confirmed diagnosis of alcoholic liver cirrhosis, hospitalized at the Internal Medicine Clinic, University Clinical Centre of the Republic of Srpska in Banja Luka, Department of Gastroenterology and Hepatology. The study period spanned from January 2023 to January 2025, with the note that patient selection and monitoring began much earlier, in June 2021. After hospitalization, patients were followed monthly through a program of outpatient control examinations, with disease outcomes recorded. The study included all male patients over 18 years of age with a confirmed diagnosis of alcoholic liver cirrhosis and signed informed consent. Female patients and those with cirrhosis or other etiologies were excluded. For statistical data analysis, the Statistical Package for the Social Sciences (SPSS) version 29 (IBM Corp., Armonk, NY, USA) was used. The statistical analyses performed included median, standard deviation, analysis of variance, Student's t-test, chi-square test, and survival analysis. Results The mean age of the patients was 59.09±9.316 years. Most of them had anemia: 113 patients (91.86%) with decreased erythrocytes and 109 patients (88.62%) with decreased hemoglobin. Thrombocytopenia was observed in 104 patients (84.55%), while an increased mean corpuscular volume (MCV) was recorded in 68 patients (55.28%). Among biochemical parameters, the most common findings were increased bilirubin in 98 patients (79.67%), aspartate aminotransferase (AST) in 111 patients (90.24%), gamma-glutamyl transferase (GGT) in 109 patients (88.61%), and D-dimer in 110 patients (89.44%), while albumin levels were decreased in 107 patients (87.00%). Hyponatremia (decreased sodium) was observed in 63 patients (51.21%), and hypercalcemia (increased calcium) in 116 patients (94.30%). Jaundice was the most common external sign, present in 98 patients (79.67%), while ascites were noted in 86 patients (69.91%). Death during the first decompensation occurred in 31 patients (25.20%), of whom 17 (54.83%) died in the hospital. The leading cause of mortality is bleeding from esophageal varices.  Conclusion Although a healthy liver performs over 200 distinct functions in the human body, a cirrhotic liver leads, one might say, to an even greater number of dysfunctions. This pathology is extremely complex, characterized by numerous complications and high treatment costs, which, despite all applied measures, do not ensure a favorable long-term prognosis without liver transplantation., Competing Interests: Human subjects: Consent for treatment and open access publication was obtained or waived by all participants in this study. University Clinical Centre of the Republika Srpska issued approval 01-19-462-2/24. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2025, Bokan et al.)

Vegetated biofiltration system (VBS) is an effective green technology for urban stormwater and greywater treatment. However, VBS is yet to be optimised for effective treatment of wastewater, particularly if it contains trace organic chemicals (TrOCs). The effect of plant species has not been addressed under TrOC wastewater loading. This study tested and evaluated the effectiveness of VBS over a one-year period in removing six TrOCs commonly found in wastewater, namely Caffeine (CAF), Paracetamol (PCM), Sulfamethoxazole (SMX), N diethyl‑meta-toluamide (DEET), Bisphenol A (BPA) and Ibuprofen (IBU). Eleven VBS configurations were tested in a year-long laboratory column study to explore the role of seven different plant species (with differing characteristic), varied soil media depths, and soil characteristics, on the fate of TrOCs in the systems. The effect of different operational conditions (e.g., dosing volume and regime) on removal efficiency was investigated. The results indicated VBS was able to maintain a high removal rate (>95 %) of CAF, BPA, and IBU throughout the experiment, followed by PCM (>79 %), SMX (50 %-80 %), and DEET (<12 %). Plant species significantly impacted the removal of SMX and DEET (p<0.05), with C. indica as the best performer. Reducing hydraulic loading rate and decreasing daily dosing volume and frequency contributed positively to the PCM, SMX, and DEET removal rates. Noticeable accumulations of SMX, DEET, and BPA (8.2, 43.1, and 54.5 ng·g-1, respectively) were detected in the filter media, particularly within the saturated zone. Higher chemical concentrations (i.e., CAF and DEET) were found in plant root tissue than in plant shoot tissue. This study offers valuable insights into VBS's design and operational aspects for removing TrOCs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Biofilters are among the most popular nature-based systems for treating stormwater and delivering multiple environmental benefits. However, as a passive system, their performance tends to be inconsistent in removing emerging organic contaminants produced by anthropogenic activities that can be persistent, mobile, and toxic. Thus, in this study, real time control (RTC) of stormwater biofilters is introduced to enhance the removal of a diverse range of organic chemicals. Laboratory columns were employed to investigate the performance of five RTC strategies, i.e., dynamic soil moisture control (RTC-Moisture), infiltration rate control (RTC-IR), pre-drain (RTC-PreDrain), fully unsaturated (RTC-UnSat), and fully saturated (RTC-FulSat). These RTC strategies were tested under varying rainfall sizes, as well as dry and wet conditions. Additionally, the study examined the accumulation of organic chemicals in the media. The results revealed that RTC-Moisture, RTC-IR, and RTC-PreDrain were the top three performing strategies, which achieved a significantly higher removal rate than Non-RTC biofilters for the majority of tested organic chemicals (p-value < 0.05). The best RTC strategy, RTC-Moisture, not only had the highest overall performance (average removal rate of 76.1 %) but was also least affected by various rainfall events. Despite a better chemical removal found in RTC-Moisture and RTC-PreDrain, there was no significant overall increase in the accumulation of organic chemicals within the media (p-value > 0.05) when compared to Non-RTC biofilters. This may indicate that the biodegradation process could be promoted in the well-performing RTC biofilters. This study confirms the possibility of using RTC strategies to enhance organic chemical removal in stormwater biofilters., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Anaerobic treatment of domestic wastewater has the advantages of lower biomass yield, lower energy demand and higher energy recover over the conventional aerobic treatment process. However, the anaerobic process has the inherent issues of excessive phosphate and sulfide in effluent and superfluous H 2 S and CO 2 in biogas. An electrochemical method allowing for in-situ generation of Fe 2+ in the anode and hydroxide ion (OH - ) and H 2 in the cathode was proposed to overcome the challenges simultaneously. The effect of electrochemically generated iron (e‑iron) on the performance of anaerobic wastewater treatment process was explored with four different dosages in this work. The results showed that compared to control, the experimental system displayed an increase of 13.4-28.4 % in COD removal efficiency, 12.0-21.3 % in CH 4 production rate, 79.8-98.5 % in dissolved sulfide reduction, 26.0-96.0 % in phosphate removal efficiency, depending on the e‑iron dosage between 40 and 200 mg Fe/L. Dosing of the e‑iron significantly upgraded the quality of produced biogas, showing a much lower CO 2 and H 2 S contents in biogas in experimental reactor than that in control reactor. The results thus demonstrated that e‑iron can significantly improve the performance of anaerobic wastewater treatment process, bringing multiple benefits with the increase of its dosage regarding effluent and biogas quality., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)

The vegetated biofiltration systems (VBS), also known as bioretentions or rain gardens, are well-established technology for treatment of urban stormwater and recently greywater, offering multiple benefits to urban environments. However, the impact of high ammonium strength wastewater (60 mg/L) on the nitrification process in these systems is not well understood. Hence, a laboratory-based column study was conducted to uncover dominant nitrification mechanisms, based on the learnings from similar onsite wastewater treatment systems. The experimental columns tested the effect of contact time (filter media depth, 150 mm, 300 mm and 700 mm), media oxygenation (active and passive) and alkalinity/pH (marble chips 5 % weight), as well as optimal operational conditions (inflow loading, concentrations, and dissolved oxygen (DO)). All nitrogen species (NH 4 + , NO 3 - , NO 2 - ), chemical oxygen demand (COD) and physical parameters (DO, pH, electrical conductivity) were monitored across seven events over thirteen weeks. The results show that dosing with 30 and 60 mg/L of NH 4 + resulted in 700 mm sand column depth to perform almost complete nitrification of NH 4 + to NO 3 - (< 90 %), while 300 mm designs achieved partial nitrification of NH 4 + to NO 2 - , likely due to limited contact time and inefficient nitrite oxidizing bacteria activity. Nitrification potential of all designs further supported that appropriate aerobic contact time is necessary for effective nitrification. Inflow concentration of NH 4 + and DO did not significantly impact nitrification performance, while reducing daily volume loading reduced NO 3 - and NO 2 - leaching. Active and passive aeration and alkalinity buffering did not positively affect ammonium removal. While there is a potential to apply both nitrification-denitrification and anammox processes to future VBS design, further understanding of aeration and alkalinity on microbially driven nitrification processes is needed., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Since the needs and expectations of communities towards their urban environments often vary, landscape management strategies can often be prone to fail in the absence of social considerations. It is therefore incumbent on policy-makers to investigate and attempt to reconcile diverse community perceptions toward the natural and built environment for more equitable governance. This is of particular interest when planning and managing nature-based solutions (NBS) for river protection. We considered this challenge in understanding human values, perceptions and behaviour in a multilayered ecosystem that includes waterways, NBS, green open spaces, and a built environment. This paper analyses perceptions and behaviour around a public urban park next to the Georges River in Sydney Australia, utilizing a proxy-based approach and a mixed-method comprising community surveys and behavioural mapping. The results showed that while users perceive the significance of the urban river environment differently, naturalistic (ecological), humanistic (recreational) and utilitarian (well-being) values are dominant. Urban river catchments are highly valued for recreational purposes, with a strong perception of potential flooding hazards. Through exploring the literature, we recognized that the dominancy of leisure-related values around urban river catchments can be generalized to similar cases worldwide. While NBS, as an urban stormwater management solution, address some user values (e.g., naturalistic) around urban river catchments, they may lack further delivery of humanistic and utilitarian values due to the poor integration with recreational and cultural spaces. It was also the case around the Georges River, where low prominence of cultural features was observed. We concluded that NBS development around Georges River and other urban river catchments should incorporate socio-cultural considerations and community values, in particular the ones related to leisure. The gaps between users' beliefs and behaviour do not greatly challenge governance, provided that the decision-makers utilise these gaps for optimising management actions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Although iron salts such as iron(III) chloride (FeCl 3 ) have widespread application in wastewater treatment, safety concerns limit their use, due to the corrosive nature of concentrated solutions. This study demonstrates that local, electrochemical generation of iron is a viable alternative to the use of iron salts. Three laboratory systems with anaerobic membrane processes were set up to treat real wastewater; two systems used the production of either in-situ or ex-situ electrochemical iron (as Fe 2+ and Fe 2+ (Fe 3+ ) 2 O 4 , respectively), while the other system served as a control. These systems were operated for over one year to assess the impact of electrochemically produced iron on system performance. The results showed that dosing of electrochemical iron significantly reduced sulfide concentration in effluent and hydrogen sulfide content in biogas, and mitigated organics-based membrane fouling, all of which are critical issues inherently related to sustainability of anaerobic wastewater treatment. The electrochemical iron strategy can generate multiple benefits for wastewater management including increased removal efficiencies for total and volatile suspended solids, chemical oxygen demand and phosphorus. The rate of methane production also increased with electrochemically produced iron. Economic analysis revealed the viability of electrochemical iron with total cost reduced by one quarter to a third compared with using FeCl 3 . These benefits indicate that electrochemical iron dosing can greatly enhance the overall operation and performance of anaerobic membrane processes, and this particularly facilitates wastewater management in a decentralized scenario., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Planning for future urban development and water infrastructure is uncertain due to changing human activities and climate. To quantify these changes, we need adaptable and fast models that can reliably explore scenarios without requiring extensive data and inputs. While such models have been recently considered for urban development, they are lacking for stormwater pollution assessment. This work proposes a novel Future Urban Stormwater Simulation (FUSS) model, utilizing a previously developed urban planning algorithm (UrbanBEATS) to dynamically assess pollution changes in urban catchments. By using minimal input data and adding stochastic point-source pollution to the build-up/wash-off approach, this study highlights calibration and sensitivity analysis of flow and pollution modules, across the range of common stormwater pollutants. The results highlight excellent fit to measured values in a continuous rainfall simulation for the flow model, with one significant calibration parameter. The pollution model was more variable, with TSS, TP and Pb showing high model efficiency, while TN was predicted well only across event-based assessment. The work further explores the framework for the model application in future pollution assessment, and points to the future work aiming to developing land-use dependent model parameter sets, to achieve flexibility for model application across varied urban catchments.

In this study, three up-flow fixed-bed bioreactors, named as A, B, and C, packed with polycaprolactone (PCL) under different filling ratios (31%, 62%, and 93%, respectively), were investigated over a long period (96 days). During the stable period, the mean effluent NO 3 - - N concentrations in reactors A, B, and C were 1.35 ± 0.50, 1.07 ± 0.41, and 1.03 ± 0.27 mg/L, respectively, which showed the removal of NO 3 - - N was not closely related to filling ratio (p > 0.05, one-way ANOVA). However, it was found that biomass in reactor A was 2.13 and 5.55 times in B and C, respectively. Excessively thick biofilm refrained the enzymatic hydrolysis of PCL and biofilm's specific denitrification rate (SDNR). Backwash stimulated organic matter release and enabled biofilm to restore its denitrification activity. The maximum cycle of backwash was 6 days for the lowest filling ratio reactor. Additionally, the utilization rates for denitrification were 83.3%, 86.4%, and 60.5% in reactors A, B, and C, respectively, which was higher after backwash than before backwash. PRACTITIONER POINTS: Excessively thick biofilm refrained the enzymatic hydrolysis of PCL. Backwash stimulated organic matter release and enabled biofilm to restore its denitrification activity. The maximum cycle of backwash was 6 days for the lowest filling ratio reactor. A higher utilization rate of PCL for denitrification was observed after backwash., (© 2021 Water Environment Federation.)

Vegetated biofiltration systems (biofilters) are now a well-established technology for treatment of urban stormwater, typically showing high nutrient uptake. However, the impact of high temporal variability of rainfall events (further exacerbated by climate change) on nitrogen and phosphorus removal processes, within different biofiltration designs, is still unknown. Hence, a laboratory-based study was conducted to uncover mechanisms behind nutrient removal in biofilters across different drying and wetting regimes. Two sets of experimental columns were based on (1) the standard biofiltration design (unsaturated zone only), and (2) combination of unsaturated and saturated (submerged) zone (SZ) with additional carbon source. Columns were watered with synthetic stormwater according to three drying and wetting schemes, exploring 1, 2, 3, 4 and 7-week drying. Hydraulic performance, soil moisture and pollutant removal were monitored. The results show that hydraulic conductivity of SZ design experiences less change over time compared to standard design, due to slower media drying, crack formation and lower plant die-off. Varied drying lengths challenged both designs differently, with 2-week drying resulting in significant drop of performance across most pollutants in standard design (except ammonia), while SZ design was able to retain high performance for up to four weeks of drying, sustaining microbial and plant uptake. Increased oxygenation of SZ columns during short-term drying was beneficial for ammonia and phosphorus removal. While SZ design showed better performance and quicker recovery for nitrogen removal, in regions with inter-rain event shorter than two weeks, the standard design (no saturated zone, no carbon source) can achieve similar if not better results., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Low energy and cost solutions are needed to combat raising water needs in urbanised areas and produce high quality recycled water. In this study, we investigated key processes that drive a unique greywater treatment train consisting of a passive green wall biofiltration system followed by disinfection using a Boron-doped diamond (BDD) electrode with a solid polymer electrolyte (SPE). In both systems, the treatment was performed without any additional chemicals and pollutants of concern were monitored for process evaluation. The green wall system removed over 90% of turbidity, apparent colour, chemical oxygen demand, total organic carbon, and biological oxygen demand, and 1 log of E. coli and total coliforms, mostly through biological processes. The green wall effluent met several proposed greywater reuse guidelines, except for E. coli and total coliform treatment (below 10 MPN/100 mL). Further disinfection of treated greywater (contained 28 mg/L Cl¯ and electrical conductivity (EC) of 181.3 µS/cm) by electrolysis at current density 25 mA/cm 2 inactivated over 3.5 logs of both E. coli and total coliforms, in 10 - 15 min of electrolysis, resulting in recycled water with less than 2 MPN/100 mL. A synergistic effect between electrochemically-generated free chlorines and reactive oxygen species contributed to the inactivation process. Although the treated water contained diluted chloride and had low EC, estimated energy consumption was just 0.63 - 0.83 kWh/m 3 . This is the first study to show the effectiveness of a low energy and a low cost greywater treatment train that combines green urban infrastructure with BDD electrochemical treatment process with SPE, offering a reliable and an environmentally-friendly method for greywater reuse., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020. Published by Elsevier Ltd.)

Green walls that effectively treat greywater have the potential to become a part of the solution for the issues of water scarcity and pollution control in our cities. To develop reliable and efficient designs of such systems, the following two research questions were addressed: what would be the optimal design of a green wall for greywater treatment, and how tall should the system be to assure adequate treatment. This paper reports on (i) a long-term pollutant removal comparison study of two typical green wall configurations: pot and block designs, and (ii) a short-term profile study exploring pollutant retention at different heights of a three-level green wall, across different plant species. Removal of suspended solids (TSS), nitrogen (TN), phosphorus (TP), chemical oxygen demand (COD) and Escherichia coli was tested, as well as various physical parameters. Pot and block designs were found to exhibit similar pollutant removal performance for standard and high inflow concentrations, while the block design was more resistant to drying. However, due to its multiple practical advantages, pot designs are favoured. The greatest removal was achieved within the top green wall level for all studied pollutants, while subsequent levels facilitated further removal of TSS, COD, and TN. Interestingly, colour, pH, and EC increased after each green wall level, which must be taken into account to determine the maximum height of these systems. The optimal size of the system was found to be dependent on plant species choice. The results were used to create practical recommendations for the effective design of greywater treatment green walls., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Green walls are becoming a popular infrastructure choice in densely built urban environments, due to their multiple benefits. However, high and vastly variable water requirements of these systems are preventing their further widespread. Only a small number of studies have investigated water needs of green walls, even though this can help to design more optimal systems with increased benefits. Additionally, the knowledge on interactions between plant uptake and climate conditions (temperature and humidity) is lacking. The aim of this study was to understand daily water requirements of five plant species (C. appressa, N. obliterata, L. muscari, M. parvifolium and O. japonicus) used in greywater treating green walls, across different seasons, temperature, and humidity conditions of temperate-oceanic climate (common in parts of Australia, US and Europe). The results showed that during summer, dominant water uptake processes were plant uptake and transpiration, resulting in three to four times higher water needs than during winter, when evaporation is a major effect. Top levels of the multi-level green wall exhibited significantly higher plant activity compared to bottom levels, showing four times greater water uptake. Temperature and humidity changes during winter caused the change in water uptake of plants, pointing to different growing and activity patterns of tested plants. During summer only N. obliterata showed temperature and humidity dependence. Annual plant water uptake and other practical recommendations are given based on the results. Even though this study focused on water requirements of greywater treating green walls, its findings can also inform traditional green wall designs., (Copyright © 2019 Elsevier Ltd. All rights reserved.)