35 results on '"Richert-Pöggeler, Katja R."'
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2. Phage tail-like particles are versatile bacterial nanomachines – A mini-review
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Patz, Sascha, Becker, Yvonne, Richert-Pöggeler, Katja R., Berger, Beatrice, Ruppel, Silke, Huson, Daniel H., and Becker, Matthias
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- 2019
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3. Mycoviruses Increase the Attractiveness of Fusarium graminearum for Fungivores and Suppress Production of the Mycotoxin Deoxynivalenol.
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Schiwek, Simon, Slonka, Matthäus, Alhussein, Mohammad, Knierim, Dennis, Margaria, Paolo, Rose, Hanna, Richert-Pöggeler, Katja R., Rostás, Michael, and Karlovsky, Petr
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FUNGAL viruses ,FUSARIUM ,DEOXYNIVALENOL ,FILAMENTOUS fungi ,RNA viruses ,MYCOTOXINS - Abstract
RNA viruses of the genera Ambivirus, Mitovirus, Sclerotimonavirus, and Partitivirus were found in a single isolate of Fusarium graminearum. The genomes of the mitovirus, sclerotimonavirus, and partitivirus were assigned to previously described viruses, whereas the ambivirus genome putatively represents a new species, named Fusarium graminearum ambivirus 1 (FgAV1). To investigate the effect of mycoviruses on the fungal phenotype, the spontaneous loss of mycoviruses during meiosis and the transmission of mycoviruses into a new strain via anastomosis were used to obtain isogenic F. graminearum strains both with and without mycoviruses. Notable effects observed in mycovirus-harboring strains were (i) the suppression of the synthesis of trichothecene mycotoxins and their precursor trichodiene, (ii) the suppression of the synthesis of the defense compound aurofusarin, (iii) the stimulation of the emission of 2-methyl-1-butanol and 3-methyl-1-butanol, and (iv) the increased attractiveness of fungal mycelia for fungivorous collembolans. The increased attractiveness of mycovirus-infected filamentous fungi to animal predators opens new perspectives on the ecological implications of the infection of fungi with viruses. [ABSTRACT FROM AUTHOR]
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- 2024
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4. The nature and organization of satellite DNAs in Petunia hybrida, related, and ancestral genomes.
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Alisawi, Osamah, Richert-Pöggeler, Katja R., Heslop-Harrison, J. S. (Pat), and Schwarzacher, Trude
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SATELLITE DNA ,KARYOTYPES ,FLUORESCENCE in situ hybridization ,PETUNIAS ,CHROMOSOMES ,GENOMES ,TANDEM repeats ,CYTOGENETICS ,TELOMERES - Abstract
Introduction: The garden petunia, Petunia hybrida (Solanaceae) is a fertile, diploid, annual hybrid species (2n=14) originating from P. axillaris and P. inflata 200 years ago. To understand the recent evolution of the P. hybrida genome, we examined tandemly repeated or satellite sequences using bioinformatic and molecular cytogenetic analysis. Methods: Raw reads from available genomic assemblies and survey sequences of P. axillaris N (PaxiN), P. inflata S6, (PinfS6), P. hybrida (PhybR27) and the here sequenced P. parodii S7 (PparS7) were used for graph and k-mer based cluster analysis of TAREAN and RepeatExplorer. Analysis of repeat specific monomer lengths and sequence heterogeneity of the major tandem repeat families with more than 0.01% genome proportion were complemented by fluorescent in situ hybridization (FISH) using consensus sequences as probes to chromosomes of all four species. Results: Seven repeat families, PSAT1, PSAT3, PSAT4, PSAT5 PSAT6, PSAT7 and PSAT8, shared high consensus sequence similarity and organisation between the four genomes. Additionally, many degenerate copies were present. FISH in P. hybrida and in the three wild petunias confirmed the bioinformatics data and gave corresponding signals on all or some chromosomes. PSAT1 is located at the ends of all chromosomes except the 45S rDNA bearing short arms of chromosomes II and III, and we classify it as a telomere associated sequence (TAS). It is the most abundant satellite repeat with over 300,000 copies, 0.2% of the genomes. PSAT3 and the variant PSAT7 are located adjacent to the centromere or mid-arm of one to three chromosome pairs. PSAT5 has a strong signal at the end of the short arm of chromosome III in P. axillaris and P.inflata, while in P. hybrida additional interstitial sites were present. PSAT6 is located at the centromeres of chromosomes II and III. PSAT4 and PSAT8 were found with only short arrays. Discussion: These results demonstrate that (i) repeat families occupy distinct niches within chromosomes, (ii) they differ in the copy number, cluster organization and homogenization events, and that (iii) the recent genome hybridization in breeding P. hybrida preserved the chromosomal position of repeats but affected the copy number of repetitive DNA. [ABSTRACT FROM AUTHOR]
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- 2023
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5. Bacteria isolated from the cuticle of plant-parasitic nematodes attached to and antagonized the root-knot nematode Meloidogyne hapla
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Topalović, Olivera, Elhady, Ahmed, Hallmann, Johannes, Richert-Pöggeler, Katja R., and Heuer, Holger
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- 2019
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6. A divergent strain of melon chlorotic spot virus isolated from black medic (Medicago lupulina) in Austria
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Gaafar, Yahya Z. A., Richert-Pöggeler, Katja R., Sieg-Müller, Angelika, Lüddecke, Petra, Herz, Kerstin, Hartrick, Jonas, Seide, Yvonne, Vetten, Heinrich-Josef, and Ziebell, Heiko
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- 2019
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7. Caraway yellows virus, a novel nepovirus from Carum carvi
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Gaafar, Yahya Z. A., Richert-Pöggeler, Katja R., Sieg-Müller, Angelika, Lüddecke, Petra, Herz, Kerstin, Hartrick, Jonas, Maaß, Christina, Ulrich, Roswitha, and Ziebell, Heiko
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- 2019
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8. Characterisation of a novel nucleorhabdovirus infecting alfalfa (Medicago sativa)
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Gaafar, Yahya Z. A., Richert-Pöggeler, Katja R., Maaß, Christina, Vetten, Heinrich-Josef, and Ziebell, Heiko
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- 2019
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9. Host range and molecular and ultrastructural analyses of Asparagus virus 1 pathotypes isolated from garden asparagus Asparagus officinalis L.
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Lantos, Edit, Krämer, Reiner, Richert-Pöggeler, Katja R., Maiss, Edgar, König, Janine, and Nothnagel, Thomas
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ASPARAGUS ,MIXED infections ,PLANT indicators ,CELLULAR inclusions ,ASPARAGACEAE - Abstract
Asparagus samples were examined from growing areas of Germany and selected European as well as North, Central and South American countries. Overall, 474 samples were analyzed for Asparagus virus 1 (AV1) using DAS-ELISA. In our survey, 19 AV1 isolates were further characterized. Experimental transmission to 11 species belonging to Aizoaceae, Amarantaceae, Asparagaceae, and Solanaceae succeeded. The ultrastructure of AV1 infection in asparagus has been revealed and has been compared with the one in indicator plants. The cylindrical inclusion (CI) protein, a core factor in viral replication, localized within the cytoplasm and in systemic infections adjacent to the plasmodesmata. The majority of isolates referred to pathotype I (PI). These triggered a hypersensitive resistance in inoculated leaves of Chenopodium spp. and were incapable of infecting Nicotiana spp. Only pathotype II (PII) and pathotype III (PIII) infected Nicotiana benthamiana systemically but differed in their virulence when transmitted to Chenopodium spp. The newly identified PIII generated amorphous inclusion bodies and degraded chloroplasts during systemic infection but not in local lesions of infected Chenopodium spp. PIII probably evolved via recombination in asparagus carrying a mixed infection by PI and PII. Phylogeny of the coat protein region recognized two clusters, which did not overlap with the CI-associated grouping of pathotypes. These results provide evidence for ongoing modular evolution of AV1. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Petuvirus : Caulimoviridae
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Richert-Pöggeler, Katja R., Tidona, Christian, editor, and Darai, Gholamreza, editor
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- 2011
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11. Plant viruses are growing in the European virus archive network
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Accotto, Gian Paolo, Candresse, Thierry, Chabannes, Matthieu, Ciuffo, Marina, Desbiez, Cécile, Gentit, Pascal, Glasa, Miroslav, Iskra Caruana, Marie-Line, Jelkmann, Wilhelm, Marais, Armelle, Menzel, Wulf, Mulabisana, Julia, Niehl, Annette, Prat, Christian, Predajna, Lukas, Richert-Pöggeler, Katja R., Romette, Jean-Louis, Rubino, Luisa, Urbino, Cica, Verdin, Eric, Ziebell, Heiko, and Zikeli, Kerstin
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- 2022
12. PVCV-like viruses : Caulimoviridae
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Richert-Pöggeler, Katja R., Tidona, Christian A., editor, Darai, Gholamreza, editor, and Büchen-Osmond, Cornelia, editor
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- 2001
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13. To Be Seen or Not to Be Seen: Latent Infection by Tobamoviruses.
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Ilyas, Rabia, Rohde, Mareike J., Richert-Pöggeler, Katja R., and Ziebell, Heiko
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LATENT infection ,TOBACCO mosaic virus ,ETIOLOGY of diseases ,PLANT viruses ,MIXED infections - Abstract
Tobamoviruses are among the most well-studied plant viruses and yet there is still a lot to uncover about them. On one side of the spectrum, there are damage-causing members of this genus: such as the tobacco mosaic virus (TMV), tomato brown rugose fruit virus (ToBRFV) and cucumber green mottle mosaic virus (CGMMV), on the other side, there are members which cause latent infection in host plants. New technologies, such as high-throughput sequencing (HTS), have enabled us to discover viruses from asymptomatic plants, viruses in mixed infections where the disease etiology cannot be attributed to a single entity and more and more researchers a looking at non-crop plants to identify alternative virus reservoirs, leading to new virus discoveries. However, the diversity of these interactions in the virosphere and the involvement of multiple viruses in a single host is still relatively unclear. For such host–virus interactions in wild plants, symptoms are not always linked with the virus titer. In this review, we refer to latent infection as asymptomatic infection where plants do not suffer despite systemic infection. Molecular mechanisms related to latent behavior of tobamoviruses are unknown. We will review different studies which support different theories behind latency. [ABSTRACT FROM AUTHOR]
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- 2022
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14. Distinct expression of endogenous Petunia vein clearing virus and the DNA transposon dTph1 in two Petunia hybrida lines is correlated with differences in histone modification and siRNA production
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Noreen, Faiza, Akbergenov, Rashid, Hohn, Thomas, and Richert-Pöggeler, Katja R.
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- 2007
15. Editorial: DNA virus and host plant interactions from antagonism to endogenization.
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Richert-Pöggeler, Katja R., Iskra-Caruana, Marie-Line, and Yuji Kishima
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DNA viruses ,PLANT viruses ,HOST plants ,PLANT DNA ,VIRAL ecology - Published
- 2022
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16. Participation of Multifunctional RNA in Replication, Recombination and Regulation of Endogenous Plant Pararetroviruses (EPRVs).
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Richert-Pöggeler, Katja R., Vijverberg, Kitty, Alisawi, Osamah, Chofong, Gilbert N., Heslop-Harrison, J. S., and Schwarzacher, Trude
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RNA ,DNA synthesis ,NON-coding RNA ,NUCLEAR DNA ,HOMOLOGOUS chromosomes ,DNA repair - Abstract
Pararetroviruses, taxon Caulimoviridae , are typical of retroelements with reverse transcriptase and share a common origin with retroviruses and LTR retrotransposons, presumably dating back 1.6 billion years and illustrating the transition from an RNA to a DNA world. After transcription of the viral genome in the host nucleus, viral DNA synthesis occurs in the cytoplasm on the generated terminally redundant RNA including inter- and intra-molecule recombination steps rather than relying on nuclear DNA replication. RNA recombination events between an ancestral genomic retroelement with exogenous RNA viruses were seminal in pararetrovirus evolution resulting in horizontal transmission and episomal replication. Instead of active integration, pararetroviruses use the host DNA repair machinery to prevail in genomes of angiosperms, gymnosperms and ferns. Pararetrovirus integration – leading to Endogenous ParaRetroViruses, EPRVs – by illegitimate recombination can happen if their sequences instead of homologous host genomic sequences on the sister chromatid (during mitosis) or homologous chromosome (during meiosis) are used as template. Multiple layers of RNA interference exist regulating episomal and chromosomal forms of the pararetrovirus. Pararetroviruses have evolved suppressors against this plant defense in the arms race during co-evolution which can result in deregulation of plant genes. Small RNAs serve as signaling molecules for Transcriptional and Post-Transcriptional Gene Silencing (TGS, PTGS) pathways. Different populations of small RNAs comprising 21–24 nt and 18–30 nt in length have been reported for Citrus, Fritillaria, Musa, Petunia, Solanum and Beta. Recombination and RNA interference are driving forces for evolution and regulation of EPRVs. [ABSTRACT FROM AUTHOR]
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- 2021
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17. Molecular characterization of a new badnavirus associated with streak symptoms on enset (Ensete ventricosum, Musaceae).
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Abraham, Adane, Winter, Stephan, Richert‐Pöggeler, Katja R., and Menzel, Wulf
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ENSETE ,BANANA diseases & pests ,POLYMERASE chain reaction ,ELECTRON microscopy - Abstract
Abstract: Electron microscopy of leaf samples displaying streak symptoms from enset (Ensete ventricosum), a banana‐like plant widely cultivated in Ethiopia, showed the presence of bacilliform shaped virions as known for badnaviruses. DNA extracts subjected to rolling circle amplification (RCA), polymerase chain reaction (PCR) and cloning and sequence analysis revealed that the virus has a circular double‐stranded DNA genome of 7,163 nucleotides encoding predicted proteins of 21.5 kDa, 14.5 kDa and 202.5 kDa, a genome organization known for badnaviruses. The virus is phylogenetically most closely related to Sugarcane bacilliform Guadeloupe D virus with a nucleotide sequence identity of 77.2% at the conserved RT/RNase‐H region and 73.6% for the whole genome. Following the current species demarcation criteria, the virus should be considered as an isolate of a new species in the genus Badnavirus for which the name Enset leaf streak virus (ELSV) is suggested. Leaf samples from enset and banana were screened using virus‐specific primers, and ELSV was detected in six of 40 enset but not found in any of 61 banana samples. On the other hand, Banana streak OL virus (BSOLV) was detected from the majority (60%) of symptomatic banana samples but not from enset samples. This paper reports the first full‐genome sequence of a putative new badnavirus species infecting plants in the genus Ensete. In addition, this is the first report of the occurrence of BSOLV in Ethiopia. [ABSTRACT FROM AUTHOR]
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- 2018
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18. Using electron microscopy to uncover latent tobamovirus.
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Ilyas, Rabia, Gaafar, Yahya Z. A., Ziebell, Heiko, and Richert-Pöggeler, Katja R.
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ELECTRON microscopy ,LATENT infection ,TOBACCO mosaic virus ,PLANT viruses ,NICOTIANA benthamiana ,SAP (Plant) ,QUINOA ,VIRUS identification - Abstract
A combination of electron microscopy and genome sequencing is highly efficient in screening for latent virus infections independent of the virus morphology or genome. One example is hoya tobamovirus-2 (HoTV-2 genbank accession number: MT750216.1) that has been reported from symptomatic mixed infected samples in 2011 and also as single, asymptomatic, infection in several Hoya species in Germany. The virus induces no symptoms in experimental host plants including Chenopodium quinoa, N. tabacum 'Xanthi nc', N. benthamiana and N. tabacum 'Samsun nn'. 14 days post inoculation, plant sap homogenates were examined using negative staining. Rod shaped virions of 300nm length were detected in newly developing leaves, confirming systemic infection. The virions' morphology and size indicated presence of a tobamovirus. The virus titer was lower when compared to tobacco mosaic virus (TMV) infected plants. For virus species identification, RNA sequencing data were scanned for virus specific sequences. The assembled virus genome could be assigned to the genus Tobamovirus. Phylogenetic analysis showed it is closely related to Youcai mosaic virus (YoMV). Therefore, polyclonal antisera for YoMV (DSMZ number AS-0527) was used in immuno-electron microscopy. The heterologous antiserum reacted with HoTV-2, while other tobamovirus antisera used in routine tobamovirus detection did not. The binding of antibodies along the virion was irregular when compared to TMV and its homologous antisera. Mixed infected Hoya plants and HoTV-2 infected N. benthamiana leaves were embedded to study the ultrastructure of infected plants displaying latency linking in situ HoTV-2 structures. [ABSTRACT FROM AUTHOR]
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- 2022
19. Evolution of Integrated Plant Viruses.
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Roossinck, Marilyn J., Hohn, Thomas, Richert-Pöggeler, Katja R., Staginnus, Christina, Harper, Glyn, Schwarzacher, Trude, Teo, Chee How, Teycheney, Pierre-Yves, Iskra-Caruana, Marie-Line, and Hull, Roger
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Plant pararetroviruses replicate their genome via a transcription-reverse transcription cycle like retroviruses, but unlike them their genomes do not obligatorily integrate into the host chromatin. Nevertheless, one can find complete or fragmented pararetrovirus genomes, as well as those from geminiviruses and even RNA viruses incorporated into the genomes of nearly all plants analysed. Integration events are thought to be rare and even rarer are those that find their way into the germ line. Normally, these integrated viral sequences are incomplete, rearranged and mutated and cannot easily escape as active viruses. However, in some cases apparently more recently acquired and therefore less initiated integrates can escape by direct transcription from tandem insertions or by recombination. This can lead to severe outbreaks in crop and ornamental plants. In anticipation of such events, methods have been developed for the detection and characterization of integrated virus sequences in plant genomes. [ABSTRACT FROM AUTHOR]
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- 2008
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20. The Julius Kühn-Institut becomes a new member of the European Virus Archive.
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Richert-Pöggeler, Katja R., Niehl, Annette, Jelkmann, Wilhelm, Pfeilstetter, Ernst, and Ziebell, Heiko
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PLANT viruses ,ARCHIVES ,ASH (Tree) ,VIRUS diseases ,ENGLISH oak ,COVID-19 - Abstract
The article offers information related to European Virus Archive (EVA) was created in 2008 in response to the need for a coordinated and readily accessible collection of human and animal viruses that could be made available to academia, public health organizations and industry. It report that EVA-GLOBAL collaborated with 38 academic institutions at the forefront of human, animal and plant research in Virology, associated with key Non- Governmental Organizations.
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- 2021
21. Endogenous pararetroviruses: two-faced travelers in the plant genome
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Staginnus, Christina and Richert-Pöggeler, Katja R.
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PLANT viruses , *PLANT chromosomes , *VIRUS diseases , *PLANT genomes , *CULTIVARS - Abstract
Endogenous plant pararetroviruses (EPRVs) were identified as integrated counterparts of most members of the plant virus family Caulimoviridae and represent repetitive elements that are ubiquitous in the plant kingdom. They are often located in pericentromeric regions of plant chromosomes in the vicinity of retrotransposon sequences. Depending on their structure and sequence integrity, some EPRVs are able to replicate and to initiate viral infection. However, conservation of integrated sequences in plant genomes might indicate benefits for the host during evolution. Understanding EPRV activation and control by the host could have important implications for plant breeding strategies to prevent viral disease caused by EPRVs in newly generated cultivars. [Copyright &y& Elsevier]
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- 2006
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22. Detection of petunia vein-clearing virus: model for the detection of DNA viruses in plants with homologous endogenous pararetrovirus sequences
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Harper, Glyn, Richert-Pöggeler, Katja R., Hohn, Thomas, and Hull, Roger
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PETUNIAS , *VIRUSES - Abstract
A number of cases of plant virus sequence integration into host plant genome have been reported. In at least two cases, endogenous pararetrovirus sequences are correlated strongly with subsequent episomal virus infection and there is circumstantial evidence that this also occurs for Petunia vein-clearing virus (PVCV). The detection of viruses is a critical component of plant health and therefore, it is important to have diagnostic procedures that differentiate between the detection of encapsidated viral DNA and homologous sequences in the host genome. PCR-based detection methods targeted at PVCV DNA have been tested and particular attention was paid to design controls that would indicate the existence of host DNA in the reaction. The use of ion-exchange chromatography for the partial purification of plant viruses from other cellular components, including chromosomal DNA, is described. The methods tested for PVCV detection are used to illustrate general principles for the specific detection of virus infections in host plants that carry homologous virus sequences in their genomes. [Copyright &y& Elsevier]
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- 2003
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23. PVCV-like viruses.
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Richert-Pöggeler, Katja R.
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PLANT viruses , *PETUNIAS , *ORNAMENTAL plants , *VIRUSES , *NUCLEOTIDE sequence - Abstract
The article presents information on petunia vein clearing virus-like viruses of the family Caulimoviridae. Their virion, genome and replication strategy are mentioned. The first description of petunia vein clearing virus in petunia appeared in 1973. Their nucleotide sequences, proteins and biology are also presented.
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- 2002
24. Exploration of the small RNA landscape in Petunia hybrida infected with latent viruses and a pospiviroid using high throughput sequencing.
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G. N., Chofong, Horres, Ralf, and Richert-Pöggeler, Katja R.
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NUCLEOTIDE sequencing ,NON-coding RNA ,PETUNIAS ,LANDSCAPES - Abstract
The article presents a study on exploration of the small RNA landscape in Petunia hybrida infected with latent viruses and a pospiviroid using high throughput sequencing. It reports the study will help to contribute to the knowledge of the collection of vsiRNAs for better reconstruction of the virome, shade light on the mechanism of vsiRNA action on host mRNA, contribute to the understanding of benefits for the host during symbiosis.
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- 2021
25. The state of plant virology research in West and Central Africa.
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Chofong, Gilbert N. and Richert-Pöggeler, Katja R.
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PLANT viruses - Abstract
The article offers information related to The Pan-African Virology Conference, which is scheduled from March 25-27, 2020, in Ghana's capital city, Accra and the mention the conference will bring together African virologists all over the world who are researching into various aspects of virology.
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- 2021
26. Insight into the evolution of the Solanaceae from the parental genomes of Petunia hybrida
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Schuurink, Robert C., Kuhlemeier, Cris, Weiss, Julia, Spelt, Kees, Yue, Zhen, Bucher, Marcel, Warner, Ryan M., Sims, Thomas L., Pezzotti, Mario, Lyons, Eric, Tang, Haibao, Qi, Qinzhou, Druege, Uwe, Zethof, Jan, Martinoia, Enrico, Borghi, Lorenzo, Nouri, Eva, Johns, Mitrick, D'Agostino, Nunzio, Vijverberg, Kitty, Egea-Cortines, Marcos, Grandont, Laurie, Heslop-Harrison, J. S., Amrad, Avichai Moshe, Muhlemann, Joëlle, Urbanus, Susan L., Villarino, Gonzalo H., Vandenbussche, Michiel, Bao, Manzhu, Passeri, Valentina, Bapaume, Laure, Davies, Kevin, Mattson, Neil S., Barry, Cornelius S., Quattrocchio, Francesca, Hintzsche, Jennifer, Schranz, M. Eric, Boersma, Maaike R., Koes, Ronald, Lv, Xiaodan, Moser, Michel, Dudareva, Natalia, Bruggmann, Rémy, Richert-Pöggeler, Katja R., Malla, Diwa, Morel, Patrice, Schatz, Michael C., Schwarzacher, Trude, Delledonne, Massimo, Bliek, Mattijs, Bombarely, Aureliano, Robbins, Tim P., Mueller, Lukas A., Fernandez-Pozo, Noe, Reinhardt, Didier, Rich, Melanie, and Franken, Philipp
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15. Life on land ,580 Plants (Botany) - Abstract
Petunia hybrida is a popular bedding plant that has a long history as a genetic model system. We report the whole-genome sequencing and assembly of inbred derivatives of its two wild parents, P. axillaris N and P. inflata S6. The assemblies include 91.3% and 90.2% coverage of their diploid genomes (1.4 Gb; 2n = 14) containing 32,928 and 36,697 protein-coding genes, respectively. The genomes reveal that the Petunia lineage has experienced at least two rounds of hexaploidization: the older gamma event, which is shared with most Eudicots, and a more recent Solanaceae event that is shared with tomato and other solanaceous species. Transcription factors involved in the shift from bee to moth pollination reside in particularly dynamic regions of the genome, which may have been key to the remarkable diversity of floral colour patterns and pollination systems. The high-quality genome sequences will enhance the value of Petunia as a model system for research on unique biological phenomena such as small RNAs, symbiosis, self-incompatibility and circadian rhythms.
27. Ortervirales: New Virus Order Unifying Five Families of Reverse-Transcribing Viruses.
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Krupovic, Mart, Blomberg, Jonas, Coffin, John M., Dasgupta, Indranil, Hung Fan, Geering, Andrew D., Gifford, Robert, Harrach, Balázs, Hull, Roger, Johnson, Welkin, Kreuze, Jan F., Lindemann, Dirk, Llorens, Carlos, Lockhart, Ben, Mayer, Jens, Muller, Emmanuelle, Olszewski, Neil E., Pappu, Hanu R., Pooggin, Mikhail M., and Richert-Pöggeler, Katja R.
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REVERSE transcriptase , *VIRUS-induced enzymes , *CAULIMOVIRIDAE , *METAVIRIDAE , *VIRAL proteins , *VIRAL genomes - Published
- 2018
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28. Identification of a new nucleopolyhedrovirus isolated from the olive leaf moth, Palpita vitrealis, from two locations in Egypt.
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El-Salamouny, Said, Wennmann, Jörg T., Kleespies, Regina G., Richert-Pöggeler, Katja R., Mansour, Amany, Awad, Mona, Agamy, Essam, Salama, Ramadan, and Jehle, Johannes A.
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OLIVE leaves , *NUCLEOPOLYHEDROVIRUSES , *MOTHS , *WHOLE genome sequencing , *BIOLOGICAL pest control agents , *OLIVE - Abstract
[Display omitted] • New baculovirus from olive moth, Palpita vitrealis, termed PaviNPV. • PaviNPV was isolated in 2005 and 2019 in Egypt in olive plantations. • PaviNPV represents a new species within group I of the Alphabaculovirus genus. • The genome is 117,533 bp and contains 131 ORFs. The olive leaf moth (jasmine moth), Palpita vitrealis (Lepidoptera: Crambidae), is an important insect pest of olives in several Mediterranean countries. A new alphabaculovirus was isolated from diseased larvae of P. vitrealis in Egypt, first in Giza in spring 2005 and again in Marsa Matrouh in 2019.The larvae exhibited typical symptoms of a baculovirus infection. Light and scanning electron microscopy studies revealed polyhedral occlusion bodies. Transmission electron microscopy of ultrathin sections of purified OBs revealed virions with multiple embedded nucleocapsids. The identity of the two virus isolates was confirmed by sequencing the partial polyhedrin and lef-8 genes, and sequence comparison suggested a relationship to group I alphabaculoviruses. Therefore, this virus was termed Palpita vitrealis nucleopolyhedrovirus (PaviNPV). Whole genome sequencing of the PaviNPV isolate from Giza (Gz05) revealed a genome of 117,533 bp, 131 open reading frames (ORFs) and four homologous repeat (hr) regions. Phylogenetic reconstruction and genetic distance analyses using 38 core genes indicated that PaviNPV should be considered to belong to a novel species within the genus Alphabaculovirus. In bioassays, PaviNPV was highly virulent against second-instar larvae of P. vitrealis. The study reports a novel baculovirus that might have potential as a biological control agent of the olive leaf moth. [ABSTRACT FROM AUTHOR]
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- 2022
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29. Insight into the evolution of the Solanaceae from the parental genomes of Petunia hybrida
- Author
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Lukas A. Mueller, T. P. Robbins, Gonzalo H. Villarino, Trude Schwarzacher, Julia Weiss, Eva Nouri, Natalia Dudareva, Jan Zethof, Kevin M. Davies, Michiel Vandenbussche, Xiaodan Lv, Marcel Bucher, Philipp Franken, Nunzio D’Agostino, Massimo Delledonne, Mélanie K. Rich, Neil S. Mattson, Eric Lyons, Patrice Morel, Mattijs Bliek, Jennifer D. Hintzsche, Ronald Koes, Mitrick A. Johns, Laurie Grandont, Susan L. Urbanus, M. Eric Schranz, Qinzhou Qi, Michael C. Schatz, Enrico Martinoia, Laure Bapaume, Robert C. Schuurink, Aureliano Bombarely, Didier Reinhardt, Rémy Bruggmann, Katja R. Richert-Pöggeler, Mario Pezzotti, Maaike R. Boersma, Michel Moser, J. S. Heslop-Harrison, Uwe Druege, Diwa Malla, Kitty Vijverberg, Lorenzo Borghi, Ryan M. Warner, Haibao Tang, Avichai Moshe Amrad, Kees Spelt, Thomas L. Sims, Valentina Passeri, Francesca Quattrocchio, Manzhu Bao, Joëlle K. Muhlemann, Marcos Egea-Cortines, Cornelius S. Barry, Zhen Yue, Noe Fernandez-Pozo, Cris Kuhlemeier, Plant Physiology (SILS, FNWI), Plant Development & (Epi)Genetics (SILS, FNWI), Department of Horticulture, University of Wisconsin-Madison, Institute of Plant Sciences, University of Bern, Huazhong Agricultural University, Department of Biology, Northern Arizona University [Flagstaff], University of Amsterdam [Amsterdam] (UvA), Universität Zürich [Zürich] = University of Zurich (UZH), Universität Bern- University of Bern [Bern], Partenaires INRAE, University of Cologne, Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria (CREA), Plant & Food Research, Leibniz Institute of Vegetable and Ornamental Crops, Leibniz Association, Purdue University, Universidad Politécnica de Cartagena, Dipartimento di Biotecnologie, University of Verona (UNIVR), Boyce Thompson Institute [Ithaca], Wageningen University and Research Centre (WUR), Department of Genetics [Leicester], University of Leicester, Department of Biological Sciences, The Open University [Milton Keynes] (OU), Northern University, Beijing Genomics Institute [Shenzhen] (BGI), School of Plant Sciences, iPlant Collaborative, University of Arizona, Cornell University [New York], Reproduction et développement des plantes (RDP), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut - Federal Research Centre for Cultivated Plants, University of Nottingham, UK (UON), Cold Spring Harbor Laboratory (CSHL), School of Plant Sciences, Radboud university [Nijmegen], Institute of Plant Science, NWO-ALW grant 022.001.018 820.02.015, Marie Curie Independent Fellowship, Swiss NSF grant 31003A_159493 31003A_135778, NCCR Plant Survival, NWO-TOP grant 854.11.006, Borsa di Studio Lanzi per Genetica Agraria, Accademia dei Lincei, CNRS ATIP-AVENIR award, Deutsche Forschungsgemeinschaft grant DR411/2-1, Bombarely, Aureliano, Moser, Michel, Amrad, Avichai, Bao, Manzhu, Bapaume, Laure, Barry, Cornelius S., Bliek, Mattij, Boersma, Maaike R., Borghi, Lorenzo, Bruggmann, Rémy, Bucher, Marcel, D'Agostino, Nunzio, Davies, Kevin, Druege, Uwe, Dudareva, Natalia, Egea-Cortines, Marco, Delledonne, Massimo, Fernandez-Pozo, Noe, Franken, Philipp, Grandont, Laurie, Heslop-Harrison, J. S., Hintzsche, Jennifer, Johns, Mitrick, Koes, Ronald, Lv, Xiaodan, Lyons, Eric, Malla, Diwa, Martinoia, Enrico, Mattson, Neil S., Morel, Patrice, Mueller, Lukas A., Muhlemann, Joëlle, Nouri, Eva, Passeri, Valentina, Pezzotti, Mario, Qi, Qinzhou, Reinhardt, Didier, Rich, Melanie, Richert-Pöggeler, Katja R., Robbins, Tim P., Schatz, Michael C., Schranz, M. Eric, Schuurink, Robert C., Schwarzacher, Trude, Spelt, Kee, Tang, Haibao, Urbanus, Susan L., Vandenbussche, Michiel, Vijverberg, Kitty, Villarino, Gonzalo H., Warner, Ryan M., Weiss, Julia, Yue, Zhen, Zethof, Jan, Quattrocchio, Francesca, Sims, Thomas L., Kuhlemeier, Cris, Huazhong Agricultural University [Wuhan] (HZAU), Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria = Council for Agricultural Research and Economics (CREA), Purdue University [West Lafayette], Università degli studi di Verona = University of Verona (UNIVR), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), and Radboud University [Nijmegen]
- Subjects
0106 biological sciences ,0301 basic medicine ,Animal Ecology and Physiology ,Plant Science ,580 Plants (Botany) ,Plant Genetics ,01 natural sciences ,Petunia ,Genome ,Petunia axillaris ,NUMBER ,Plant evolution ,SYNTHASE ,Genetics ,biology ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Biosystematiek ,Life Sciences & Biomedicine ,Solanaceae ,Genome, Plant ,Evolution, Genetics, Plant evolution, Plant sciences ,EXPRESSION ,DATABASE ,Evolution ,SEQUENCE ,Evolution, Molecular ,Polyploidy ,03 medical and health sciences ,Genetic model ,Life Science ,Gene ,SUPPRESSION ,Whole genome sequencing ,Science & Technology ,Plant Sciences ,15. Life on land ,biology.organism_classification ,GENE ,030104 developmental biology ,POLLINATOR ATTRACTION ,TRANSPOSABLE ELEMENTS ,Biosystematics ,Hybridization, Genetic ,EPS ,Plant sciences ,GENERATION ,010606 plant biology & botany - Abstract
Petunia hybrida is a popular bedding plant that has a long history as a genetic model system. We report the whole-genome sequencing and assembly of inbred derivatives of its two wild parents, P. axillaris N and P. inflata S6. The assemblies include 91.3% and 90.2% coverage of their diploid genomes (1.4 Gb; 2n = 14) containing 32,928 and 36,697 protein-coding genes, respectively. The genomes reveal that the Petunia lineage has experienced at least two rounds of hexaploidization: the older gamma event, which is shared with most Eudicots, and a more recent Solanaceae event that is shared with tomato and other solanaceous species. Transcription factors involved in the shift from bee to moth pollination reside in particularly dynamic regions of the genome, which may have been key to the remarkable diversity of floral colour patterns and pollination systems. The high-quality genome sequences will enhance the value of Petunia as a model system for research on unique biological phenomena such as small RNAs, symbiosis, self-incompatibility and circadian rhythms.
- Published
- 2016
30. Biological and Genetic Characterization of Physostegia Chlorotic Mottle Virus in Europe Based on Host Range, Location, and Time.
- Author
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Temple C, Blouin AG, De Jonghe K, Foucart Y, Botermans M, Westenberg M, Schoen R, Gentit P, Visage M, Verdin E, Wipf-Scheibel C, Ziebell H, Gaafar YZA, Zia A, Yan XH, Richert-Pöggeler KR, Ulrich R, Rivarez MPS, Kutnjak D, Vučurović A, and Massart S
- Subjects
- Phylogeny, Plant Diseases, Ecosystem, Serbia, Host Specificity, Solanum lycopersicum
- Abstract
Application of high throughput sequencing (HTS) technologies enabled the first identification of Physostegia chlorotic mottle virus (PhCMoV) in 2018 in Austria. Subsequently, PhCMoV was detected in Germany and Serbia on tomatoes showing severe fruit mottling and ripening anomalies. We report here how prepublication data-sharing resulted in an international collaboration across eight laboratories in five countries, enabling an in-depth characterization of PhCMoV. The independent studies converged toward its recent identification in eight additional European countries and confirmed its presence in samples collected 20 years ago (2002). The natural plant host range was expanded from two to nine species across seven families, and we confirmed the association of PhCMoV presence with severe fruit symptoms on economically important crops such as tomato, eggplant, and cucumber. Mechanical inoculations of selected isolates in the greenhouse established the causality of the symptoms on a new indexing host range. In addition, phylogenetic analysis showed a low genomic variation across the 29 near-complete genome sequences available. Furthermore, a strong selection pressure within a specific ecosystem was suggested by nearly identical sequences recovered from different host plants through time. Overall, this study describes the European distribution of PhCMoV on multiple plant hosts, including economically important crops on which the virus can cause severe fruit symptoms. This work demonstrates how to efficiently improve knowledge on an emergent pathogen by sharing HTS data and provides a solid knowledge foundation for further studies on plant rhabdoviruses.[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
- Published
- 2022
- Full Text
- View/download PDF
31. ICTV Virus Taxonomy Profile: Caulimoviridae .
- Author
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Teycheney PY, Geering ADW, Dasgupta I, Hull R, Kreuze JF, Lockhart B, Muller E, Olszewski N, Pappu H, Pooggin MM, Richert-Pöggeler KR, Schoelz JE, Seal S, Stavolone L, Umber M, and Report Consortium I
- Subjects
- Genome, Viral, Plants virology, Virus Replication, Caulimoviridae classification, Caulimoviridae physiology, Caulimoviridae ultrastructure
- Abstract
Caulimoviridae is a family of non-enveloped reverse-transcribing plant viruses with non-covalently closed circular dsDNA genomes of 7.1-9.8 kbp in the order Ortervirales . They infect a wide range of monocots and dicots. Some viruses cause economically important diseases of tropical and subtropical crops. Transmission occurs through insect vectors (aphids, mealybugs, leafhoppers, lace bugs) and grafting. Activation of infectious endogenous viral elements occurs in Musa balbisiana , Petunia hybrida and Nicotiana edwardsonii . However, most endogenous caulimovirids are not infectious. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Caulimoviridae, which is available at ictv.global/report/caulimoviridae.
- Published
- 2020
- Full Text
- View/download PDF
32. Complete genome sequence and construction of an infectious full-length cDNA clone of celery latent virus - an unusual member of a putative new genus within the Potyviridae.
- Author
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Rose H, Döring I, Vetten HJ, Menzel W, Richert-Pöggeler KR, and Maiss E
- Subjects
- 3' Untranslated Regions, 5' Untranslated Regions, Agrobacterium tumefaciens genetics, Genetic Vectors, Italy, Open Reading Frames, Plant Diseases virology, Polyproteins genetics, Potyviridae isolation & purification, RNA, Viral genetics, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Nicotiana, Viral Proteins genetics, Apium virology, DNA, Complementary, Potyviridae genetics, Potyviridae growth & development
- Abstract
Celery latent virus (CeLV) is an incompletely described plant virus known to be sap and seed transmissible and to possess flexuous filamentous particles measuring about 900 nm in length, suggesting it as a possible member of the family Potyviridae. Here, an Italian isolate of CeLV was transmitted by sap to a number of host plants and shown to have a single-stranded and monopartite RNA genome being 11 519 nucleotides (nts) in size and possessing some unusual features. The RNA contains a large open reading frame (ORF) that is flanked by a short 5' untranslated region (UTR) of 13 nt and a 3' UTR consisting of 586 nt that is not polyadenylated. CeLV RNA shares nt sequence identity of only about 40 % with other members of the Potyviridae (potyvirids). The CeLV polyprotein is notable in that it starts with a signal peptide, has a putative P3N-PIPO ORF and shares low aa sequence identity (about 18 %) with other potyvirids. Although potential cleavage sites were not identified for the N-terminal two-thirds of the polyprotein, the latter possesses a number of sequence motifs, the identity and position of which are characteristic of other potyvirids. Attempts at constructing an infectious full-length cDNA clone of CeLV were successful following Rhizobium radiobacter infiltration of Nicotiana benthamiana and Apium graveolens. CeLV appears to have the largest genome of all known potyvirids and some unique genome features that may warrant the creation of a new genus, for which we propose the name 'celavirus'.
- Published
- 2019
- Full Text
- View/download PDF
33. Electron Microscopy Methods for Virus Diagnosis and High Resolution Analysis of Viruses.
- Author
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Richert-Pöggeler KR, Franzke K, Hipp K, and Kleespies RG
- Abstract
The term "virosphere" describes both the space where viruses are found and the space they influence, and can extend to their impact on the environment, highlighting the complexity of the interactions involved. Studying the biology of viruses and the etiology of virus disease is crucial to the prevention of viral disease, efficient and reliable virus diagnosis, and virus control. Electron microscopy (EM) is an essential tool in the detection and analysis of virus replication. New EM methods and ongoing technical improvements offer a broad spectrum of applications, allowing in-depth investigation of viral impact on not only the host but also the environment. Indeed, using the most up-to-date electron cryomicroscopy methods, such investigations are now close to atomic resolution. In combination with bioinformatics, the transition from 2D imaging to 3D remodeling allows structural and functional analyses that extend and augment our knowledge of the astonishing diversity in virus structure and lifestyle. In combination with confocal laser scanning microscopy, EM enables live imaging of cells and tissues with high-resolution analysis. Here, we describe the pivotal role played by EM in the study of viruses, from structural analysis to the biological relevance of the viral metagenome (virome).
- Published
- 2019
- Full Text
- View/download PDF
34. Insight into the evolution of the Solanaceae from the parental genomes of Petunia hybrida.
- Author
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Bombarely A, Moser M, Amrad A, Bao M, Bapaume L, Barry CS, Bliek M, Boersma MR, Borghi L, Bruggmann R, Bucher M, D'Agostino N, Davies K, Druege U, Dudareva N, Egea-Cortines M, Delledonne M, Fernandez-Pozo N, Franken P, Grandont L, Heslop-Harrison JS, Hintzsche J, Johns M, Koes R, Lv X, Lyons E, Malla D, Martinoia E, Mattson NS, Morel P, Mueller LA, Muhlemann J, Nouri E, Passeri V, Pezzotti M, Qi Q, Reinhardt D, Rich M, Richert-Pöggeler KR, Robbins TP, Schatz MC, Schranz ME, Schuurink RC, Schwarzacher T, Spelt K, Tang H, Urbanus SL, Vandenbussche M, Vijverberg K, Villarino GH, Warner RM, Weiss J, Yue Z, Zethof J, Quattrocchio F, Sims TL, and Kuhlemeier C
- Subjects
- Polyploidy, Evolution, Molecular, Genome, Plant, Hybridization, Genetic, Petunia genetics
- Abstract
Petunia hybrida is a popular bedding plant that has a long history as a genetic model system. We report the whole-genome sequencing and assembly of inbred derivatives of its two wild parents, P. axillaris N and P. inflata S6. The assemblies include 91.3% and 90.2% coverage of their diploid genomes (1.4 Gb; 2n = 14) containing 32,928 and 36,697 protein-coding genes, respectively. The genomes reveal that the Petunia lineage has experienced at least two rounds of hexaploidization: the older gamma event, which is shared with most Eudicots, and a more recent Solanaceae event that is shared with tomato and other solanaceous species. Transcription factors involved in the shift from bee to moth pollination reside in particularly dynamic regions of the genome, which may have been key to the remarkable diversity of floral colour patterns and pollination systems. The high-quality genome sequences will enhance the value of Petunia as a model system for research on unique biological phenomena such as small RNAs, symbiosis, self-incompatibility and circadian rhythms.
- Published
- 2016
- Full Text
- View/download PDF
35. Induction of infectious petunia vein clearing (pararetro) virus from endogenous provirus in petunia.
- Author
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Richert-Pöggeler KR, Noreen F, Schwarzacher T, Harper G, and Hohn T
- Subjects
- Base Sequence, Biolistics, DNA Methylation, DNA Primers, DNA, Viral genetics, Genome, Viral, Open Reading Frames, Plant Diseases virology, Proviruses physiology, Virus Integration, Petunia virology, Plant Viruses genetics, Retroviridae genetics
- Abstract
Infection by an endogenous pararetrovirus using forms of both episomal and chromosomal origin has been demonstrated and characterized, together with evidence that petunia vein clearing virus (PVCV) is a constituent of the Petunia hybrida genome. Our findings allow comparative and direct analysis of horizontally and vertically transmitted virus forms and demonstrate their infectivity using biolistic transformation of a provirus-free petunia species. Some integrants within the genome of P.hybrida are arranged in tandem, allowing direct release of virus by transcription. In addition to known inducers of endogenous pararetroviruses, such as genome hybridization, tissue culture and abiotic stresses, we observed activation of PVCV after wounding. Our data also support the hypothesis that the host plant uses DNA methylation to control the endogenous pararetrovirus.
- Published
- 2003
- Full Text
- View/download PDF
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