262 results on '"Ropka-Molik, Katarzyna"'
Search Results
2. First transcriptomic insight into the working muscles of racing pigeons during a competition flight
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Stefaniuk-Szmukier, Monika, Szmatoła, Tomasz, Pustelnik, Agnieszka, and Ropka-Molik, Katarzyna
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- 2024
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3. Characteristic of Przewalski horses population from Askania-Nova reserve based on genetic markers
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Musiał, Adrianna D., Ropka-Molik, Katarzyna, Stefaniuk-Szmukier, Monika, Myćka, Grzegorz, Bieniek, Agnieszka, and Yasynetska, Nataliya
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- 2023
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4. Gene Expression Level and Immunohistochemical Localization of Cannabinoid and Cannabinoid-Related Receptors in The Small Intestine of Holstein Bulls (Bos Taurus Taurus).
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Osiak-Wicha, Cezary, Muszyński, Siemowit, Tomaszewska, Ewa, Kras, Katarzyna, Ropka-Molik, Katarzyna, Zhyla, Mykola, and Arciszewski, Marcin Bartłomiej
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CANNABINOID receptors ,SMALL intestine ,SUBMUCOUS plexus ,TRPV cation channels ,CATTLE ,GENE expression ,GASTROINTESTINAL motility disorders ,MILK yield - Abstract
The gastrointestinal tract plays a crucial role in nutrient absorption, secretion, and motility, ensuring proper digestion and overall homeostasis. Regulation of this complex system involves the coordination of various communication pathways, including neural and humoral mechanisms. One such mechanism is the endocannabinoid system (ECS), a signaling network comprising endogenous cannabinoids, receptors, and enzymes involved in the regulation of physiological processes in mammals and non-mammalian species. While extensive research has been conducted on the ECS in monogastric animals, limited information is available on its presence and distribution in cattle. This study aimed to investigate the distribution and localization patterns of cannabinoid receptors type 1 (CB1R) and type 2 (CB2R) and transient receptor potential vanilloid type 1 (TRPV1) in the bovine small intestine. The study included immunohistochemical analysis of intestinal tissue samples from Polish Holstein-Friesian breed bulls. Gene expression levels of CNR1, CNR2, and TRPV1 genes, encoding CB1R, CB2R, and TRPV1, respectively, were quantified using qPCR analysis. The results showed that all three receptors were expressed in the bovine small intestine, with TRPV1 exhibiting a significant upregulation in the jejunum compared to the duodenum and ileum. Immunoreactivity for CB1R and CB2R was predominantly observed in neurons of the enteric plexuses, while TRPV1 immunolabeling was detected in both enteric neurons and duodenal Brunner's glands. These findings may establish an anatomical foundation for further investigations, lending support to the potential therapeutic efficacy of cannabinoid receptor agonists in alleviating gastrointestinal motility disorders associated with bovine enteropathies and optimizing milk production in dairy cattle. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Mitochondrial DNA and Y chromosome reveal the genetic structure of the native Polish Konik horse population.
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Musiał, Adrianna Dominika, Radović, Lara, Stefaniuk-Szmukier, Monika, Bieniek, Agnieszka, Wallner, Barbara, and Ropka-Molik, Katarzyna
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CHROMOSOME analysis ,Y chromosome ,HYPERVARIABLE regions ,HAPLOGROUPS ,MITOCHONDRIAL DNA ,HORSE breeding ,HORSE breeds - Abstract
Polish Konik remains one of the most important horse breeds in Poland. The primitive, native horses with a stocky body and mouse-like coat color are protected by a conservation program, while their Polish population consists of about 3,480 individuals, representing 16 dam and six sire lines. To define the population's genetic structure, mitochondrial DNA and Y chromosome sequence variables were identified. The mtDNA whole hypervariable region analysis was carried out using the Sanger sequencing method on 233 Polish Koniks belonging to all dam lines, while the Y chromosome analysis was performed with the competitive allele-specific PCR genotyping method on 36 horses belonging to all sire lines. The analysis of the mtDNA hypervariable region detected 47 SNPs, which assigned all tested horses to 43 haplotypes. Most dam lines presented more than one haplotype; however, five dam lines were represented by only one haplotype. The haplotypes were classified into six (A, B, E, J, G, R) recognized mtDNA haplogroups, with most horses belonging to haplogroup A, common among Asian horse populations. Y chromosome analysis allocated Polish Koniks in the Crown group, condensing all modern horse breeds, and divided them into three haplotypes clustering with coldblood breeds (28 horses), warmblood breeds (two horses), and Duelmener Pony (six horses). The clustering of all Wicek sire line stallions with Duelmener horses may suggest a historical relationship between the breeds. Additionally, both mtDNA and Y chromosome sequence variability results indicate crossbreeding before the studbooks closure or irregularities in the pedigrees occurred before the DNA testing introduction. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Insight into the genetic diversity of Selle Français horse populations based on whole D-loop sequencing of mitochondrial DNA.
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ROPKA-MOLIK, Katarzyna, AYAD, Abdelhanine, MUSIAŁ, Adriana D., BESSEBOUA, Omar, AISSANOU, Sofiane, STEFANIUK-SZMUKIER, Monika, and PIÓRKOWSKA, Katarzyna
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- 2024
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7. Molecular insights into the lipid‐carbohydrates metabolism switch under the endurance effort in Arabian horses.
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Myćka, Grzegorz, Ropka‐Molik, Katarzyna, Cywińska, Anna, Szmatoła, Tomasz, and Stefaniuk‐Szmukier, Monika
- Abstract
Copyright of Equine Veterinary Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2024
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8. ACTN3 genotype distribution across horses representing different utility types and breeds
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Musiał, Adrianna D., Ropka-Molik, Katarzyna, Piórkowska, Katarzyna, Jaworska, Joanna, and Stefaniuk-Szmukier, Monika
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- 2019
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9. The expression profile of genes involved in osteoclastogenesis detected in whole blood of Arabian horses during 3 years of competing at race track
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Stefaniuk-Szmukier, Monika, Ropka-Molik, Katarzyna, Piórkowska, Katarzyna, and Bugno-Poniewierska, Monika
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- 2019
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10. Comprehensive analysis of runs of homozygosity and heterozygosity in Holstein cattle on the basis of medium and high density SNP panels and large population sample.
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Szmatoła, Tomasz, Gurgul, Artur, Jasielczuk, Igor, and Ropka-Molik, Katarzyna
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HOLSTEIN-Friesian cattle ,INBREEDING ,HOMOZYGOSITY ,SINGLE nucleotide polymorphisms ,HETEROZYGOSITY ,CATTLE genetics ,CATTLE breeding ,DENSITY - Abstract
This study reports runs of homozygosity (ROH) and heterozygosity (ROHet) distributed in a large population of Holstein cattle on the basis of two microarrays of medium (50k; 2163 animals; 54 609 SNPs) and high single nucleotide polymorphism (SNP) density (HD; 600 animals; 777 692 SNPs). To assess the inbreeding values of Holstein cattle, the ROH-based genomic inbreeding coefficient (F
ROH ) was calculated. The comparison of SNP panels suggested that FROH values above 4 Mb should be considered for panels of medium densities as a relatively reliable measure of inbreeding. Moreover, ROH hotspots and coldspots were identified and compared between the HD and 50k SNP panels and were carefully examined for association with production and functional traits. The obtained results pinpointed genomic regions presumably under selection pressure in Holstein cattle. The regions overlapped with a large number of genes, including GHR, GBF1, SUMF1, CCL28, NIM1K, U6, BTRC and FABP1, many of which are involved in important Holstein cattle characteristics. We also found that some ROH hotspots and coldspots identified with the HD panel were not detected with the 50k panel, mainly because of insufficient SNP density in certain genomic regions. This suggests that using medium-density panels might not be the best choice when precise identification of ROH patterns is the main goal. In summary, in this work, we confirmed that a high-density SNP panel compared to a medium-density SNP panel allows for more precise identification of ROH patterns, especially in the case of short ROH that could be associated with ancestral inbreeding. [ABSTRACT FROM AUTHOR]- Published
- 2024
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11. Variation in TBX3 Gene Region in Dun Coat Color Polish Konik Horses
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Stefaniuk-Szmukier, Monika, Ropka-Molik, Katarzyna, Piórkowska, Katarzyna, Szmatoła, Tomasz, Długosz, Bogusława, Pisarczyk, Weronika, and Bugno-Poniewierska, Monika
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- 2017
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12. Identification of candidate genes and regulatory factors related to growth rate through hypothalamus transcriptome analyses in broiler chickens
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Piórkowska, Katarzyna, Żukowski, Kacper, Połtowicz, Katarzyna, Nowak, Joanna, Ropka-Molik, Katarzyna, Derebecka, Natalia, Wesoły, Joanna, and Wojtysiak, Dorota
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- 2020
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13. Expression of Genes Encoding Selected Orexigenic and Anorexigenic Peptides and Their Receptors in the Organs of the Gastrointestinal Tract of Calves and Adult Domestic Cattle (Bos taurus taurus).
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Kras, Katarzyna, Ropka-Molik, Katarzyna, Muszyński, Siemowit, and Arciszewski, Marcin B.
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CATTLE , *GENE expression , *PEPTIDES , *HOLSTEIN-Friesian cattle , *CATTLE breeds , *CALVES , *LEPTIN receptors , *GASTROINTESTINAL system - Abstract
The regulation of food intake occurs at multiple levels, and two of the components of this process are orexigenic and anorexigenic peptides, which stimulate or inhibit appetite, respectively. The study of the function of these compounds in domestic cattle is essential for production efficiency, animal welfare, and health, as well as for economic benefits, environmental protection, and the contribution to a better understanding of physiological aspects that can be applied to other species. In this study, the real-time PCR method was utilized to determine the expression levels of GHRL, GHSR, SMIM20, GPR173, LEP, LEPR, and NUCB2 (which encode ghrelin, its receptor, phoenixin-14, its receptor, leptin, its receptor, and nesfatin-1, respectively) in the gastrointestinal tract (GIT) of Polish Holstein–Friesian breed cattle. In all analyzed GIT segments, mRNA for all the genes was present in both age groups, confirming their significance in these tissues. Gene expression levels varied distinctly across different GIT segments and between young and mature subjects. The differences between calves and adults were particularly pronounced in areas such as the forestomachs, ileum, and jejunum, indicating potential changes in peptides regulating food intake based on the developmental phase. In mature individuals, the forestomachs predominantly displayed an increase in GHRL expression, while the intestines had elevated levels of GHSR, GPR173, LEP, and NUCB2. In contrast, the forestomachs in calves showed upregulated expressions of LEP, LEPR, and NUCB2, highlighting the potential importance of peptides from these genes in bovine forestomach development. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Immunoexpression of Spexin in Selected Segments of the Bovine (Bos taurus taurus) Gastrointestinal Tract.
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Dajnowska, Aleksandra, Osiak-Wicha, Cezary, Piech, Małgorzata, Muszyński, Siemowit, Tomaszewska, Ewa, Ropka-Molik, Katarzyna, Krzysiak, Michał K., and Arciszewski, Marcin B.
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SUBMUCOUS plexus ,GASTROINTESTINAL system ,CATTLE ,ENTERIC nervous system ,SMALL intestine ,HOLSTEIN-Friesian cattle - Abstract
Simple Summary: Our research delved into the exploration of spexin, a neuropeptide, within the stomach, small intestine, and colon of cows. While the roles of spexin in smaller animals are established, our focus was to bridge the knowledge gap regarding its functions in larger mammals. Through the analysis of selected segments of the unique gastrointestinal tract of the cattle, we sought to unravel the locations of spexin in the gastrointestinal tract of this polygastric animal. Remarkably, our findings revealed heightened gene expression of a specific spexin receptor, galanin receptor type 2, in the small intestine. This finding provides valuable insights into the functioning of spexin in larger livestock animals, particularly in terms of digestive regulation. The implications extend beyond veterinary considerations, potentially providing a window into understanding digestive processes in humans. This study enhances our overall understanding of neuropeptide functions and establishes a foundation for progress in animal health research. In the expansive domain of neuropeptide investigation, spexin (SPX) has emerged as a captivating subject, exerting a significant impact on diverse physiological processes. Initially identified in mice, SPX's distribution transcends various organs, suggesting its potential regulatory roles. Despite extensive research in smaller species, a notable gap exists in our comprehension of SPX in larger mammals, particularly ruminants. Our study meticulously explores the immunolocalization of SPX within the gastrointestinal organs of bovines, with a specific focus on the abomasum, jejunum, and colon. Tissue samples from Holstein–Friesian cattle underwent careful processing, and gene mRNA expression levels, particularly GALR2 and SPX, were assessed. Intriguingly, our findings revealed that GALR2 expression was highest in the jejunum, signifying a potentially critical role in this digestive segment. Immunohistochemistry further unveiled distinct patterns of SPX immunoreactivity in each examined region—abomasum, jejunum, and colon—highlighting nuanced, region-specific responses. Notably, the abomasum and jejunum predominantly exhibited positive immunoreactivity in the submucosal plexus, while the colon, in contrast, demonstrated a higher degree of immunoreactivity in myenteric plexus neurons. Our investigation, grounded in the hypothesis of ubiquitous SPX distribution in ruminants, delves deeper into the intricate role of SPX within the enteric nervous system. This study meticulously explores the spatial distribution of SPX within the myenteric and submucosal plexuses, integral components of the enteric nervous system. These findings significantly enhance our understanding of SPX's potential roles in gastrointestinal regulation in bovines, providing a unique perspective on larger mammals and enriching our comprehension of this intriguing neuropeptide's significance in various physiological processes. [ABSTRACT FROM AUTHOR]
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- 2023
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15. The use of the SLC16A1 gene as a potential marker to predict race performance in Arabian horses
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Ropka-Molik, Katarzyna, Stefaniuk-Szmukier, Monika, Szmatoła, Tomasz, Piórkowska, Katarzyna, and Bugno-Poniewierska, Monika
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- 2019
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16. Equine Metabolic Syndrome: A Complex Disease Influenced by Multifactorial Genetic Factors.
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Stefaniuk-Szmukier, Monika, Piórkowska, Katarzyna, and Ropka-Molik, Katarzyna
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METABOLIC syndrome ,SYMPTOMS ,VETERINARY medicine ,LIPID metabolism ,REGENERATIVE medicine ,HORSE breeding - Abstract
Equine metabolic syndrome (EMS) has become an important issue in modern veterinary medicine and is linked to the common, extremely painful, most-of-the-time performance-terminating hoof laminitis. The growing knowledge in the field of genetic background, inducing environmental factors, diagnosis, treatment and maintenance of affected equines led us to summarise the available information to be used not only for scientific purposes but for fieldwork. In horses, the clinical presentation of EMS includes: obesity or local fat deposition, bilateral lameness or hoof rings attributed to ongoing or previous (pasted) laminitis with the key feature of the occurrence of insulin dysregulation, disturbing the homeostasis within insulin, glucose and lipid metabolism. The management of EMS is based on dietary and fitness discipline; however, intensive research is ongoing in the field of regenerative medicine to develop modern and promising therapies. [ABSTRACT FROM AUTHOR]
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- 2023
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17. Repetitive Cerulein-Induced Chronic Pancreatitis in Growing Pigs—A Pilot Study.
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Tomaszewska, Ewa, Świątkiewicz, Małgorzata, Muszyński, Siemowit, Donaldson, Janine, Ropka-Molik, Katarzyna, Arciszewski, Marcin B., Murawski, Maciej, Schwarz, Tomasz, Dobrowolski, Piotr, Szymańczyk, Sylwia, Dresler, Sławomir, and Bonior, Joanna
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INTESTINAL mucosa ,CHRONIC pancreatitis ,TUMOR necrosis factors ,INFLAMMATORY bowel diseases ,GAMMA-glutamyltransferase ,WESTERN immunoblotting ,PANCREATIC enzymes ,MONOCARBOXYLATE transporters - Abstract
Chronic pancreatitis (CP) is an irreversible and progressive inflammatory disease. Knowledge on the development and progression of CP is limited. The goal of the study was to define the serum profile of pro-inflammatory cytokines and the cell antioxidant defense system (superoxidase dismutase—SOD, and reduced glutathione—GSH) over time in a cerulein-induced CP model and explore the impact of these changes on selected cytokines in the intestinal mucosa and pancreatic tissue, as well as on selected serum biochemical parameters. The mRNA expression of CLDN1 and CDH1 genes, and levels of Claudin-1 and E-cadherin, proteins of gut barrier, in the intestinal mucosa were determined via western blot analysis. The study showed moderate pathomorphological changes in the pigs' pancreas 43 days after the last cerulein injection. Blood serum levels of interleukin (IL)-1-beta, IL-6, tumor necrosis factor alpha (TNF-alpha), C-reactive protein (CRP), lactate dehydrogenase (LDH), gamma-glutamyl transpeptidase (GGTP), SOD and GSH were increased following cerulein injections. IL-1-beta, IL-6, TNF-alpha and GSH were also increased in jejunal mucosa and pancreatic tissue. In duodenum, decreased mRNA expression of CDH1 and level of E-cadherin and increased D-lactate, an indicator of leaky gut, indicating an inflammatory state, were observed. Based on the current results, we can conclude that repetitive cerulein injections in growing pigs not only led to CP over time, but also induced inflammation in the intestine. As a result of the inflammation, the intestinal barrier was impaired. [ABSTRACT FROM AUTHOR]
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- 2023
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18. A genome-wide detection of selection signatures in conserved and commercial pig breeds maintained in Poland
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Gurgul, Artur, Jasielczuk, Igor, Ropka-Molik, Katarzyna, Semik-Gurgul, Ewelina, Pawlina-Tyszko, Klaudia, Szmatoła, Tomasz, Szyndler-Nędza, Magdalena, Bugno-Poniewierska, Monika, Blicharski, Tadeusz, Szulc, Karolina, Skrzypczak, Ewa, and Krupiński, Jędrzej
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- 2018
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19. RNA sequencing as a powerful tool in searching for genes influencing health and performance traits of horses
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Stefaniuk, Monika and Ropka-Molik, Katarzyna
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- 2016
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20. Profiling of genetic markers useful for breeding decision in Selle Francais horse
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Ayad, Abdelhanine, Besseboua, Omar, Aissanou, Sofiane, Stefaniuk-Szmukier, Monika, Piórkowska, Katarzyna, Musiał, Adriana D., Długosz, Boguslawa, Kozłowska, Agnieszka, and Ropka-Molik, Katarzyna
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- 2022
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21. Effect of EGF, AREG and LIF genes polymorphisms on reproductive traits in pigs
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Mucha, Aurelia, Ropka-Molik, Katarzyna, Piórkowska, Katarzyna, Tyra, Mirosław, and Oczkowicz, Maria
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- 2013
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22. Association of ghrelin gene polymorphisms with slaughter traits in pig.
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Tyra, Mirosław, Ropka-Molik, Katarzyna, Piórkowska, Katarzyna, Szyndler-Nędza, Magdalena, Małopolska, Martyna, Babicz, Marek, Mucha, Aurelia, Żak, Grzegorz, and Eckert, Robert
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GENETIC polymorphisms , *GHRELIN receptors , *GHRELIN , *LANDRACE swine , *SWINE , *HAMMING weight , *SLAUGHTERING , *BODY weight - Abstract
It has been hypothesized that mutations in the ghrelin gene in pigs may play a similar role as in humans and may be associated with obesity. The aim of study was to analyse the polymorphisms of ghrelin gene and to evaluate its effect on pigs' carcass traits. The effect of c.-93A>G, 4428T>C and g.4486C>T polymorphisms at the ghrelin gene on slaughter performance was analysed in 346 gilts represented by three breeds (Polish Landrace, Duroc, Pietrain). Animals were fattened from 30 to 100 (±2.5) kg body weight. After slaughter, the carcasses were chilled for 24 hours (4ºC), weighed and the right half-carcasses were dissected and evaluated. A number of data were obtained including: meat weight in primary cuts, weight of ham, backfat thickness and carcass yield. From breeding and production point of view, the favourable results were obtained for pigs with the GG genotype at the c.-93A>G locus, characterized by better carcass results than those with the AA genotype, e.g. higher ham weight and lower average backfat thickness. In pigs with the TT genotype at the g.4428T>C locus, we found lower mean backfat thickness than in pigs with the CC genotype. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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23. The Effect of BSCL2 Gene on Fat Deposition Traits in Pigs.
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Piórkowska, Katarzyna, Sroka, Julia, Żukowski, Kacper, Zygmunt, Karolina, Ropka-Molik, Katarzyna, and Tyra, Mirosław
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SWINE breeding ,SWINE farms ,SWINE ,ADIPOSE tissues ,GENETIC variation ,GENETIC markers ,FAT - Abstract
Simple Summary: The study evaluated the mutation effects of BSCL2 variants on slaughter and fattening characteristics and meat quality traits. These mutations were selected based on variant calling analysis and χ test results within subcutaneous fat RNA-seq data. Potential genetic markers revealed significant genotype/allele distribution variations between high- and low-fat pigs. We suggested that the proposed variant calling derived from RNA-seq data might help develop genetic markers for complex pig traits. The results pinpoint that the selection of the BSCL2 G allele (rs341493267) could increase backfat thickness in pigs. In addition, observed polymorphisms also affected meat percentage, loin mass, and eye area. BSCL2 encodes seipin, a transmembrane endoplasmic reticulum protein associated with lipodystrophy and severe metabolic complications, including diabetes and hepatic steatosis. In pigs, BSCL2 expression increases during adipocyte differentiation. In the present study, we identified significant gene variants associated with fat deposition (FD)-related processes based on subcutaneous fat tissue RNA-seq data. In the association study, to prove our hypothesis, three Polish pig breeds were included: Złotnicka White (ZW, n = 72), Polish Landrace (PL, n = 201), and Polish Large White (PLW, n = 169). Based on variant calling analysis and χ
2 tests, BSCL2 mutations showing significantly different genotype/allele distribution between high- and low-fat pigs were selected for a comprehensive association study. Four interesting BSCL2 variants (rs346079334, rs341493267, rs330154033, and rs81333153) belonging to downstream and missense mutations were investigated. Our study showed a significant decrease in minor allele frequency for two BSCL2 variants (rs346079334 and rs341493267) in PL pigs in 2020–2021. In ZW, BSCL2 mutations significantly affected loin and ham fats, meat redness, and growth performance traits, such as feed conversion and daily feed intake. Similar observations were noted for PLW and PL, where BSCL2 mutations influenced fat depositions and meat traits, such as loin eye area, loin mass and fat, carcass yield, and growth performance traits. Based on the observation in pigs, our study supports the theory that BSCL2 expressed in subcutaneous fat is involved in the FD process. [ABSTRACT FROM AUTHOR]- Published
- 2023
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24. Assessment and Distribution of Runs of Homozygosity in Horse Breeds Representing Different Utility Types.
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Szmatoła, Tomasz, Gurgul, Artur, Jasielczuk, Igor, Oclon, Ewa, Ropka-Molik, Katarzyna, Stefaniuk-Szmukier, Monika, Polak, Grazyna, Tomczyk-Wrona, Iwona, and Bugno-Poniewierska, Monika
- Subjects
HORSE breeds ,HORSE breeding ,HOMOZYGOSITY ,ZINC-finger proteins ,ANIMAL species ,MUSCLE growth - Abstract
Simple Summary: In recent years, the concept of runs of homozygosity (ROH) has become increasingly important in the genetic studies of various animal species, due to its advantages in estimating inbreeding and identifying regions under selection pressure. In this study, ROH distribution in six horse breeds belonging to three horse types (primitive, light, and draft) was examined. The presented results showed diverse differences in the length, number, and frequency of ROH between the analyzed breeds. Moreover, genomic inbreeding coefficients (F
ROH ) showed a higher level of inbreeding in light and primitive horses in comparison to draft horses. Regarding ROH islands, which are regions of the genome characterized by a high frequency of ROH and thus may represent signals of selection events, we observed several genes with confirmed effects on major horse breed characteristics. In addition, ROH regions of zero frequency were analyzed and these regions also spanned across genes involved in important breed characteristics. The presented results, especially regarding ROH islands and no-ROH regions, can be used as a basis for further research concerning the identification of genes and markers that determine important horse breed characteristics. The present study reports runs of homozygosity (ROH) distribution in the genomes of six horse breeds (571 horses in total) representing three horse types (primitive, light, and draft horses) based on the 65k Equine BeadChip assay. Of major interest was the length, quantity, and frequency of ROH characteristics, as well as differences between horse breeds and types. Noticeable differences in the number, length and distribution of ROH between breeds were observed, as well as in genomic inbreeding coefficients. We also identified regions of the genome characterized by high ROH coverage, known as ROH islands, which may be signals of recent selection events. Eight to fourteen ROH islands were identified per breed, which spanned multiple genes. Many were involved in important horse breed characteristics, including WFIKNN2, CACNA1G, STXBP4, NOG, FAM184B, QDPR, LCORL, and the zinc finger protein family. Regions of the genome with zero ROH occurrences were also of major interest in specific populations. Depending on the breed, we detected between 2 to 57 no-ROH regions and identified 27 genes in these regions that were common for five breeds. These genes were involved in, e.g., muscle contractility (CACNA1A) and muscle development (miR-23, miR-24, miR-27). To sum up, the obtained results can be furthered analyzed in the topic of identification of markers unique for specific horse breed characteristics. [ABSTRACT FROM AUTHOR]- Published
- 2022
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25. Analysis of the associations between polymorphisms in GNAS complex locus and growth, carcass and meat quality traits in pigs
- Author
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Oczkowicz, Maria, Ropka-Molik, Katarzyna, and Tyra, Mirosław
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- 2013
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26. Distribution of JAK2 genotypes across Suffolk and Pomeranian sheep
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Padzik, Nicola, Szewczuk, Małgorzata, and Ropka-Molik, Katarzyna
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- 2021
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27. New long-non coding RNAs related to fat deposition based on pig model.
- Author
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Piórkowska, Katarzyna, Żukowski, Kacper, Ropka-Molik, Katarzyna, and Tyra, Mirosław
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GENETIC regulation ,LINCRNA ,FAT ,ADIPOSE tissues ,RNA ,GLYCANS ,SWINE - Abstract
Obesity has been a problem in the last decades since the development of certain technologies has led to a faster pace of life, resulting in nutritional changes. Domestic pigs are an excellent animal model in recognition of adiposity-related processes, corresponding to the size of individual organs, the distribution of body fat in the organism, and similar metabolism. The present study applied next-generation sequencing to identify adipose tissue (AT) transcriptomic signals related to increased fat content by identifying differentially expressed genes (DEGs), including long non-coding RNAs in Złotnicka White pigs (n=16). Moreover, besides commonly used functional analysis, we applied the Freiburg RNA tool to predict DE lncRNA targets based on calculation hybridisation energy. And in addition, DE lncRNAs were recognized based on information available in databases. The obtained results show that close to 230 gene expressions were found to be dependent on fat content, including 8 lncRNAs. The most interesting was that among identified DE lncRNAs was transcript corresponding to human MALAT1, which was previously considered in the obesity-related context. Moreover, it was determined that in ENSSSCG00000048394, ENSSSCG00000047210, ENSSSCG00000047442 and ENSSSCG00000041577 lncRNAs are contained repeat insertion domains of LncRNAs (RIDLs) considered as important gene expression regulatory elements, and ENSSSCG00000041577 seems to be the host for mir1247(NR_031649.1). The analysis of energy hybridisation between DE lncRNAs and DEGs using the Freiburg IntaRNAv2 tool, including isoforms expressed in AT, showed that ENSSSCG00000047210 lncRNA interacted with the highest number of DEGs and ENSSSCG00000047210 expression was only correlated with positive fat-related DEGs. The functional analysis showed that down-regulated DEGs involved in ECM proteoglycan pathways could be under the control of both positive and negative fat-related lncRNAs. The present study, using pigs as an animal model, expands current knowledge of possible gene expression regulation by lncRNAs in fat tissue and indicates for MALAT1 role in the fat deposition determination, whose function is still often questioned or doubtful. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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28. PLN Gene Analysis in Horses: Multiway Approach for the Investigation and Validation of Molecular Variation.
- Author
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MYĆKA, Grzegorz, MUSIAŁ, Adrianna D., STEFANIUK-SZMUKIER, Monika, DŁUGOSZ, Bogusława, PIÓRKOWSKA, Katarzyna, BIENIEK, Agnieszka, SZMATOŁA, Tomasz, and ROPKA-MOLIK, Katarzyna
- Published
- 2022
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29. Mitochondrial Whole D-Loop Variability in Polish Draft Horses of Sztumski Subtype.
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Myćka, Grzegorz, Klecel, Weronika, Stefaniuk-Szmukier, Monika, Jaworska, Joanna, Musiał, Adrianna Dominika, and Ropka-Molik, Katarzyna
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MITOCHONDRIA ,HORSES ,HAPLOGROUPS ,STALLIONS ,GENETIC variation - Abstract
Simple Summary: The Polish draft horse (PDH) breed is a result of crossing local mares with imported cold-blooded stallions. In this study, we investigate the genetic composition of the PDH by analyzing whole mitochondrial d-loop variabilities and comparing these variabilities to previously demonstrated whole d-loop sequences of other cold-blooded breeds: Ardennais, Belgian, Breton, Clydesdale, Noriker, Norwegian Fjord, Percheron, and Suffolk. Our results show high nucleotide diversity among the PDH population and the existence of two main haplogroups with strong kinship to the Belgian breed. The Polish draft horse (PDH) breed is a result of crossing local mares with imported cold-blooded stallions, such as Belgians, Ardennes, Fjords, and others. A part of the broodmare stock investigated in this study was also imported from various countries, such as Denmark. In this study, we investigate the genetic composition of the PDH by analyzing the whole mitochondrial d-loop variability and comparing it to previously demonstrated whole d-loop sequences of other cold-blooded breeds: Ardennais, Belgian, Breton, Clydesdale, Noriker, Norwegian Fjord, Percheron, and Suffolk. Our results show high nucleotide diversity within the PDH population (π = 0.011), and the existence of two main haplogroups: one of relatively concise origin, with strong kinship to the Belgian breed, and the second showing close relation to the majority of other analyzed cold-blooded breeds. Some of the PDH maternal strains clustered separately, which can be a result of the influence of other unidentified breeds that served as a foundation stock for the present population. This present study explains the genetic relationship of the PDH to other cold-blooded breeds and indicates the high genetic diversity of the breed. [ABSTRACT FROM AUTHOR]
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- 2022
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30. Variations in Fibrinogen-like 1 (FGL1) Gene Locus as a Genetic Marker Related to Fat Deposition Based on Pig Model and Liver RNA-Seq Data.
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Piórkowska, Katarzyna, Żukowski, Kacper, Ropka-Molik, Katarzyna, and Tyra, Mirosław
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GENETIC markers ,RNA sequencing ,MEAT flavor & odor ,SWINE ,SWINE breeding ,FILAGGRIN ,FAT - Abstract
The goal of this study was to evaluate the effects of mutations in the FGL1 gene associated with pig productive traits to enrich the genetic marker pool for further selection and to support the studies on FGL1 in the context of the fat deposition (FD) process. The variant calling and χ
2 analyses of liver RNA-seq data were used to indicate genetic markers. FGL1 mutations were genotyped in the Złotnicka White (n = 72), Polish Large White (n = 208), Duroc (n = 72), Polish Landrace (PL) (n = 292), and Puławska (n = 178) pig breeds. An association study was performed using a general linear model (GLM) implemented in SAS® software. More than 50 crucial mutations were identified in the FGL1 gene. The association study showed a significant effect of the FGL1 on intramuscular fat (IMF), loin eye area, backfat thickness at the lumbar, ham mass (p = 0.0374), meat percentage (p = 0.0205), and loin fat (p = 0.0003). Alternate homozygotes and heterozygotes were found in the PL and Duroc, confirming the selective potential for these populations. Our study supports the theory that liver FGL1 is involved in the FD process. Moreover, since fat is the major determinant of flavor development in meat, the FGL1 rs340465447_A allele can be used as a target in pig selection focused on elevated fat levels. [ABSTRACT FROM AUTHOR]- Published
- 2022
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31. Variability of mRNA abundance of leukemia inhibitory factor gene (LIF) in porcine ovary, oviduct and uterus tissues
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Ropka-Molik, Katarzyna, Oczkowicz, Maria, Mucha, Aurelia, Piórkowska, Katarzyna, and Piestrzyńska-Kajtoch, Agata
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- 2012
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32. A) in Different Horse Breeds from Europe and the United States
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Reiter, Simone, Wallner, Barbara, Brem, Gottfried, Haring, Elisabeth, Hoelzle, Ludwig, Stefaniuk-Szmukier, Monika, Długosz, Bogusława, Piórkowska, Katarzyna, Ropka-Molik, Katarzyna, Malvick, Julia, Penedo, Maria Cecilia T., and Bellone, Rebecca R.
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warmblood fragile foal syndrome ,PLOD 1 ,Arabians ,museum sample - Abstract
Warmblood fragile foal syndrome (WFFS) is an autosomal recessive disorder caused by a single nucleotide variant in the procollagen-lysine-2-oxoglutarate-5-dioxygenase 1 gene (PLOD1:c.2032G>, A, p.Gly678Arg). Homozygosity for the PLOD1 variant causes an Ehler-Danlos-like syndrome, which has to date only been reported in warmblood breeds but the WFFS allele has been also detected in the Thoroughbred. To investigate the breed distribution of the WFFS allele, 4081 horses belonging to 38 different breeds were screened. In total, 4.9% of the horses representing 21 breeds carried the WFFS allele. The affected breeds were mainly warmbloods, with carrier frequency as high as 17% in the Hanoverian and Danish Warmblood. The WFFS allele was not detected in most non-warmblood breeds. Exceptions include WFFS carriers in the Thoroughbred (17/716), Haflinger (2/48), American Sport Pony (1/12), and Knabstrupper (3/46). The origin of the WFFS allele remains unknown. The Arabian breed and specifically the stallion Bairactar Or. Ar. (1813), whose offspring were reported to have a similar phenotype in the 19th century, were hypothesized as the origin. DNA from a museum sample of Bairactar Or. Ar. showed that he did not carry the mutated allele. This result, together with the genotypes of 302 Arabians, all homozygous for the reference allele, does not support an Arabian origin of the WFFS allele. Our extensive survey shows the WFFS allele to be of moderate frequency and concern in warmbloods and also in breeds where it may not be expected.
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- 2020
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33. Variation in the SLC16A1 and the ACOX1 Genes Is Associated with Gallop Racing Performance in Arabian Horses
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Fontanel, Marie, Todd, Evelyn, Drabbe, Alize, Ropka-Molik, Katarzyna, Stefaniuk-Szmukier, Monika, Myćka, Grzegorz, and Velie, Brandon D.
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- 2020
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34. Comprehensive analysis of the whole transcriptomes from two different pig breeds using RNA-Seq method
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Ropka-Molik, Katarzyna, Żukowski, Kacper, Eckert, Robert, Gurgul, Artur, Piórkowska, Katarzyna, and Oczkowicz, Maria
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- 2014
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35. Gene expression adjustment of inflammatory mechanisms in dairy cow mammary gland parenchyma during host defense against staphylococci.
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Korwin-Kossakowska, Agnieszka, Ropka-Molik, Katarzyna, Ząbek, Tomasz, Szmatoła, Tomasz, Lewczuk, Dorota, Kościuczuk, Ewa, Marczak, Sylwester, and Bagnicka, Emilia
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MAMMARY glands , *DAIRY cattle , *MASTITIS , *GENE expression , *STAPHYLOCOCCUS , *HOLSTEIN-Friesian cattle , *BOVINE mastitis , *HEALTH of cattle - Abstract
The aim of the study was to identify differences in the expression of splice variants of the PRMT2, LTF and C4A genes in the mammary glands of healthy dairy cows and those infected with staphylococci. An expression study was conducted on 38 Polish Holstein-Friesian dairy cows who were removed from the herd owing to subclinical and chronic mastitic or reproductive issues. Two days before slaughter, milk samples were taken for microbiological analysis and examined for the presence of bacteria. The mammary gland parenchyma samples with a predominance of secretory tissue were taken; these were divided into three groups according to the health status of the mammary gland: H (without pathogenic bacteria in milk), CoNS (with coagulase-negative staphylococci in milk), and CoPS (with coagulase-positive staphylococci in milk). Two of the investigated genes, LTF and C4A, demonstrated variants unequivocally expressed in infected tissue. Two LTF gene variants were found to be associated with cow health status, and with the type of bacteria causing mastitis (CoPS or CoNS). In addition, the expression of C4A isoforms differed with regard to mastitis etiology groups. The comprehensive evaluation of PRMT2 transcript suggested that the gene may also be involved in course of mastitis: two of four PRMT2 transcripts showed increased expression in the mammary gland of the CoPS group compared to controls. The obtained results are important for the knowledge on the etiology of bovine mastitis. The effects of the identified mastitis-relevant splice variants need to be further explored on the protein level to verify the suitability of splice variants and recognize their contribution towards the disease phenotypes and course. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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36. Effect of the polymorphism of genes related to lactogenesis in pigs on colostrum and milk composition and on rearing performance of piglets.
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Szyndler-Nędza, Magdalena, Mucha, Aurelia, Ropka-Molik, Katarzyna, and Piórkowska, Katarzyna
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COMPOSITION of milk ,COLOSTRUM ,GENETIC polymorphisms ,SWINE breeding ,PIGLETS ,ANIMAL weaning ,SWINE ,LACTATION ,BODY weight - Abstract
The aim of the study was a search for new polymorphisms in the genes related to lactogenesis, including lactose synthesis in colostrum and milk, and determine their relationship with the content of colostrum and milk components and body weight of the piglets. Colostrum and milk were sampled during the second lactation on d 1, 7, 14 and 21 from 112 sows of Polish Large White (PLW) and Polish Landrace (PL). The piglets' rearing performance was determined based on the number and body weight at 1, 7, 14 and 21 d of age. In the study six polymorphisms located in different genes were analysed. Among the six polymorphisms under analysis, those identified in the B4GALT1 genes had the most significant effect on colostrum and milk composition and the piglets' body weight changes. Sows of the B4GALT1
GG genotype, when compared to those of B4GALT1AA , produced colostrum and milk with a significantly lower lactose content and reared piglets with a substantially higher body weight (by 0.30 kg) at 21 d of age. [ABSTRACT FROM AUTHOR]- Published
- 2022
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37. Tracking the Molecular Scenarios for Tumorigenic Remodeling of Extracellular Matrix Based on Gene Expression Profiling in Equine Skin Neoplasia Models.
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Podstawski, Przemysław, Ropka-Molik, Katarzyna, Semik-Gurgul, Ewelina, Samiec, Marcin, Skrzyszowska, Maria, Podstawski, Zenon, Szmatoła, Tomasz, Witkowski, Maciej, and Pawlina-Tyszko, Klaudia
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SOMATIC cell nuclear transfer , *EXTRACELLULAR matrix , *GENE expression profiling , *CELL adhesion , *BENIGN tumors , *EQUINE influenza , *TISSUE scaffolds , *TUMORS - Abstract
An important component of tissues is the extracellular matrix (ECM), which not only forms a tissue scaffold, but also provides the environment for numerous biochemical reactions. Its composition is strictly regulated, and any irregularities can result in the development of many diseases, including cancer. Sarcoid is the most common skin cancer in equids. Its formation results from the presence of the genetic material of the bovine papillomavirus (BPV). In addition, it is assumed that sarcoid-dependent oncogenic transformation arises from a disturbed wound healing process, which may be due to the incorrect functioning of the ECM. Moreover, sarcoid is characterized by a failure to metastasize. Therefore, in this study we decided to investigate the differences in the expression profiles of genes related not only to ECM remodeling, but also to the cell adhesion pathway, in order to estimate the influence of disturbances within the ECM on the sarcoid formation process. Furthermore, we conducted comparative research not only between equine sarcoid tissue bioptates and healthy skin-derived explants, but also between dermal fibroblast cell lines transfected and non-transfected with a construct encoding the E4 protein of the BP virus, in order to determine its effect on ECM disorders. The obtained results strongly support the hypothesis that ECM-related genes are correlated with sarcoid formation. The deregulated expression of selected genes was shown in both equine sarcoid tissue bioptates and adult cutaneous fibroblast cell (ACFC) lines neoplastically transformed by nucleofection with gene constructs encoding BPV1-E1^E4 protein. The identified genes (CD99, ITGB1, JAM3 and CADM1) were up- or down-regulated, which pinpointed the phenotypic differences from the backgrounds noticed for adequate expression profiles in other cancerous or noncancerous tumors as reported in the available literature data. Unravelling the molecular pathways of ECM remodeling and cell adhesion in the in vivo and ex vivo models of epidermal/dermal sarcoid-related cancerogenesis might provide powerful tools for further investigations of genetic and epigenetic biomarkers for both silencing and re-initiating the processes of sarcoid-dependent neoplasia. Recognizing those biomarkers might insightfully explain the relatively high capacity of sarcoid-descended cancerous cell derivatives to epigenomically reprogram their nonmalignant neoplastic status in domestic horse cloned embryos produced by somatic cell nuclear transfer (SCNT). [ABSTRACT FROM AUTHOR]
- Published
- 2022
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38. Screening for candidate genes related with histological microstructure, meat quality and carcass characteristic in pig based on RNA-seq data
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Ropka-Molik, Katarzyna, Bereta, Anna, Żukowski, Kacper, Tyra, Mirosław, Piórkowska, Katarzyna, Żak, Grzegorz, and Oczkowicz, Maria
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0301 basic medicine ,Candidate gene ,Population ,lcsh:Animal biochemistry ,Locus (genetics) ,Single-nucleotide polymorphism ,Quantitative trait locus ,Biology ,Article ,03 medical and health sciences ,Polymorphism ,education ,lcsh:QP501-801 ,Fibers Types ,lcsh:SF1-1100 ,Genetics ,education.field_of_study ,food and beverages ,Phenotypic trait ,QTLs ,Animal Breeding and Genetics ,Candidate Genes ,030104 developmental biology ,Genetic marker ,Animal Science and Zoology ,Histological Traits ,Intramuscular fat ,lcsh:Animal culture ,Food Science - Abstract
Objective The aim of the present study was to identify genetic variants based on RNA-seq data, obtained via transcriptome sequencing of muscle tissue of pigs differing in muscle histological structure, and to verify the variants’ effect on histological microstructure and production traits in a larger pig population. Methods RNA-seq data was used to identify the panel of single nucleotide polymorphisms (SNPs) significantly related with percentage and diameter of each fiber type (I, IIA, IIB). Detected polymorphisms were mapped to quantitative trait loci (QTLs) regions. Next, the association study was performed on 944 animals representing five breeds (Landrace, Large White, Pietrain, Duroc, and native Puławska breed) in order to evaluate the relationship of selected SNPs and histological characteristics, meat quality and carcasses traits. Results Mapping of detected genetic variants to QTL regions showed that chromosome 14 was the most overrepresented with the identification of four QTLs related to percentage of fiber types I and IIA. The association study performed on a 293 longissimus muscle samples confirmed a significant positive effect of transforming acidic coiled-coil-containing protein 2 (TACC2) polymorphisms on fiber diameter, while SNP within forkhead box O1 (FOXO1) locus was associated with decrease of diameter of fiber types IIA and IIB. Moreover, subsequent general linear model analysis showed significant relationship of FOXO1, delta 4-desaturase, sphingolipid 1 (DEGS1), and troponin T2 (TNNT2) genes with loin ‘eye’ area, FOXO1 with loin weight, as well as FOXO1 and TACC2 with lean meat percentage. Furthermore, the intramuscular fat content was positively associated (p
- Published
- 2018
39. Assessment of BPV-1 Mediated Matrix Metalloproteinase Genes Deregulation in the In Vivo and In Vitro Models Designed to Explore Molecular Nature of Equine Sarcoids.
- Author
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Podstawski, Przemysław, Ropka-Molik, Katarzyna, Semik-Gurgul, Ewelina, Samiec, Marcin, Skrzyszowska, Maria, Podstawski, Zenon, Szmatoła, Tomasz, Witkowski, Maciej, and Pawlina-Tyszko, Klaudia
- Subjects
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GENETIC regulation , *MATRIX metalloproteinases , *EXTRACELLULAR matrix proteins , *SARCOIDOSIS , *CYTOSKELETAL proteins - Abstract
Matrix metalloproteinases (MMPs) represent a family of enzymes capable of biocatalytically breaking down the structural and functional proteins responsible for extracellular matrix (ECM) integrity. This capability is widely used in physiological processes; however, imbalanced MMP activity can trigger the onset and progression of various pathological changes, including the neoplasmic transformation of different cell types. We sought to uncover molecular mechanisms underlying alterations in transcriptional profiles of genes coding for MMPs, which were comprehensively identified in equine adult dermal tissue bioptates, sarcoid-derived explants, and ex vivo expanded adult cutaneous fibroblast cell (ACFC) lines subjected to inducible oncogenic transformation into sarcoid-like cells. The results strongly support the hypothesis that the transcriptional activity of MMP genes correlates with molecular modifications arising in equine dermal cells during their conversion into sarcoid cells. The alterations in MMP transcription signatures occurs in both sarcoid tissues and experimentally transformed equine ACFC lines expressing BPV1-E4^E1 transgene, which were characterized by gene up- and down-regulation patterns. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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40. Genetic screening for cerebellar abiotrophy, severe combined immunodeficiency and lavender foal syndrome in Arabian horses in Poland
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Bugno-Poniewierska, Monika, Stefaniuk-Szmukier, Monika, -Kajtoch, Agata Piestrzyńska, Fornal, Agnieszka, Piórkowska, Katarzyna, and Ropka-Molik, Katarzyna
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- 2019
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41. Nutritional modification of SCD, ACACA and LPL gene expressions in different ovine tissues
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Ropka-Molik, Katarzyna, Knapik, Jan, Pieszka, Marek, Szmatoła, Tomasz, and Piórkowska, Katarzyna
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lcsh:Agriculture ,lcsh:Zoology ,lcsh:S ,lcsh:Q ,lcsh:Animal culture ,lcsh:QL1-991 ,lcsh:Science ,lcsh:SF1-1100 - Abstract
Fatty acid composition is one of the main factors affecting health benefits of food. Stearoyl-CoA desaturase 1 (SCD), acetyl-CoA carboxylase alpha (ACACA) and lipoprotein lipase (LPL) have been considered as the rate-limiting enzymes in the biosynthesis of different fatty acids critical in lipid metabolism. The aim of our study was the analysis of differences in expression profiles of three ovine genes related to lipid metabolism (LPL, ACACA, SCD) depending on feeding system and tissue type. The gene expression measurement was performed using a real-time PCR method on 60 old-type Polish Merino Sheep, which were divided into three feeding groups (I – complete pellet mixture, n = 12; II – complete mixture with addition of fresh grass, n = 24; III – complete mixture with addition of fresh red clover, n = 24). From all lambs, tissue samples – subcutaneous fat, perirenal fat and liver – were collected immediately after slaughter and LPL, ACACA and SCD expression was estimated based on two endogenous controls (RPS2 – ribosomal protein S2; ATP5G2 – H(+)-transporting ATP synthase). Our research indicated that supplementation of diet with an addition of fresh grass or red clover significantly (P SCD, ACACA and LPL genes in fat tissue compared to standard complete pelleted mixture. On the other hand, the highest expression of ACACA was detected in liver tissue collected from sheep fed a diet with an addition of fresh red clover (P SCD gene was detected in animals fed with grass supplementation (P SCD transcript abundance was detected in perirenal fat, while LPL and ACACA expression was the highest in both perirenal and subcutaneous fat. The ability of nutrigenomic regulation of transcription of analyzed genes confirmed that these genes play a critical role in regulation of lipid metabolism processes in sheep and could be associated with fatty acid profiles in milk and meat.
- Published
- 2017
42. Hypothalamus–pituitary axis transcriptomic modification dependent on growth rate in geese (Anser anser domesticus).
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Piórkowska, Katarzyna, Żukowski, Kacper, Połtowicz, Katarzyna, Witarski, Wojciech, Nowak, Joanna, Potocka, Paulina, and Ropka‐Molik, Katarzyna
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TRANSCRIPTOMES ,GEESE ,HYPOTHALAMUS ,GENETIC regulation ,FOS oncogenes - Abstract
Summary: The hypothalamus–pituitary axis is involved in digest processing, stress response, energy storage and many other processes. In birds, this control differs from in mammals, such as regulation of appetite and satiety centre. The transcriptomics analyses of both brain structures can explain and identify the molecular processes related to body growth and development and nutritional status. Many reports describe chicken transcriptome in literature, but gene expression studies in the other poultry species are extremely rare. Therefore, the present research undertook the attempt to explain hypothalamus–pituitary processes in domestic geese—Polish White Kołuda®, main Polish line. After 16 weeks of fattening, significant differences in geese weight were observed. Therefore, transcriptome of pituitary and hypothalamus profiles could be compared between low and high growth rate geese groups. Due to the lack of domestic geese genome assembly in the public databases, we used three mapping approaches: de novo analysis, mapping to two other pink‐footed and swan geese genomes. The functional examination showed that the most enriched biological process in the geese hypothalamus covered the immune response. Moreover, in the hypothalamus, proteins typical for the pituitary such as PRL and GH were differentially expressed (DE). Our study recommends one gene as a candidate for growth rate in geese—the FOS gene, which encodes Fos proto‐oncogene—DE in both analysed tissues. The FOS gene is involved in regulating feeding behaviour, immune regulation, stimulating cellular proliferation and controlling growth hormone synthesis. Moreover, the present investigation indicates DE genes involved in gene expression regulation. The study delivers new information about the changes in the pituitary–hypothalamic axis in geese dependent on growth rate differences. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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43. The Potential Role of MYOM1 and ATGL Genes in Pig Production Improvement.
- Author
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Ropka-Molik, Katarzyna, Piórkowska, Katarzyna, Piestrzyńska-Kajtoch, Agata, Fornal, Agnieszka, Zyskowska, Zofia, Pruś, Angelika, and Tyra, Mirosław
- Subjects
- *
SWINE , *GENETIC markers , *MEAT quality , *ANIMAL carcasses , *GENES , *PORK - Abstract
In the present study, two missense variants within ATGL (rs331307082) and MYOM1 (rs326001585) genes were tested for their potential usage as genetic markers related to pig production traits. The genotyping was performed on 519 pigs representing 990 synthetic sire line. The association analysis indicated that ATGL gene affected the panel of fattening parameters (test daily gain, age at slaughter), meatiness traits (meat percentage in the carcasses; the weight of loin, ham and primary cuts, and loin eye area), and meat quality characteristics (water exudation). In turn, MYOM1 polymorphism was related to loin weight, the weight of primary cuts and weight of loin backfat. Pigs with AA genotype were characterized by significantly higher loin and primary cut weights compared to opposite homozygotes GG (P<0.05). The observed differences were 2.29 kg and 1.2 kg, respectively. Moreover, despite higher meatiness, AA animals together with AG were characterized by lower weight of loin backfat (P<0.05) and average backfat thickness (P<0.1) compared to GG pigs. The MYOM1 polymorphism did not affect pork quality traits. The results allowed us to propose the new genetic markers which may be used in pig selection to obtain appropriate meatiness and fatness level in carcasses without decreasing meat quality. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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44. Expression Profile of Brain Aging and Metabolic Function are Altered by Resveratrol or _-Ketoglutarate Supplementation in Rats Fed a High-Fat Diet.
- Author
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Szczurek-Janicka, Paulina, Ropka-Molik, Katarzyna, Oczkowicz, Maria, Orczewska-Dudek, Sylwia, Pietras, Mariusz, and Pieszka, Marek
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HIGH-fat diet , *GENE expression profiling , *RESVERATROL , *HYPOTHALAMUS , *BLOOD sugar , *MIDDLE age , *AGING , *SIRTUINS - Abstract
The aim of this study was to examine the impact of different dietary interventions started at middle age on the metabolic phenotype and gene expression profiling in the hypothalamus. One-year old rats were fed either a control diet, high-fat diet (HFD), HFD supplemented with resveratrol (HFD+RESV), or HFD supplemented with α-ketoglutarate (HFD+AKG). A 6-week HFD feeding led to significant changes in concentrations of plasma glucose, insulin, lipids, and thyroid hormones. Moreover, 32% of the 84 analyzed genes correlated with aging were differentially expressed compared to the control group, with the largest functional class being related to inflammatory response. Dietary RESV ameliorated the changes in plasma glucose, total cholesterol, and triiodothyronine concentrations induced by HFD feeding and significantly downregulated 60% of the surveyed genes compared to the control group, resulting in a major molecular shift compared to HFD alone. In contrast, AKG supplementation did not affect the metabolic phenotype, but prevented the gene expression pattern caused by HFD consumption, mimicking the effects observed in the control group. HFD feeding induces metabolic dysfunction and age-related genetic alterations in the hypothalamus of middle-aged rats, while dietary RESV or AKG may partially retard these effects, even though these compounds act in a different and specific manner. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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45. Genomic landscape of copy number variation and copy neutral loss of heterozygosity events in equine sarcoids reveals increased instability of the sarcoid genome
- Author
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Pawlina-Tyszko, Klaudia, Gurgul, Artur, Szmatoła, Tomasz, Ropka-Molik, Katarzyna, Semik-Gurgul, Ewelina, Klukowska-Rötzler, Jolanta, Koch, Christoph, Mählmann, Kathrin, and Bugno-Poniewierska, Monika
- Subjects
630 Agriculture ,610 Medicine & health - Abstract
Although they are the most common neoplasms in equids, sarcoids are not fully characterized at the molecular level. Therefore, the objective of this study was to characterize the landscape of structural rearrangements, such as copy number variation (CNV) and copy neutral loss of heterozygosity (cnLOH), in the genomes of sarcoid tumor cells. This information will not only broaden our understanding of the characteristics of this genome but will also improve the general knowledge of this tumor and the mechanisms involved in its generation. To this end, Equine SNP64K Illumina microarrays were applied along with bioinformatics tools dedicated for signal intensity analysis. The analysis revealed increased instability of the genome of sarcoid cells compared with unaltered skin tissue samples, which was manifested by the prevalence of CNV and cnLOH events. Many of the identified CNVs overlapped with the other research results, but the simultaneously observed variability in the number and sizes of detected aberrations indicated a need for further studies and the development of more reliable bioinformatics algorithms. The functional analysis of genes co-localized with the identified aberrations revealed that these genes are engaged in vital cellular processes. In addition, a number of these genes directly contribute to neoplastic transformation. Furthermore, large numbers of cnLOH events identified in the sarcoids suggested that they may play no less significant roles than CNVs in the carcinogenesis of this tumor. Thus, our results indicate the importance of cnLOH and CNV in equine sarcoid oncogenesis and present a direction of future research.
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- 2017
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46. Identification of mRNA Degradome Variation Dependent on Divergent Muscle Mass in Different Pig Breeds.
- Author
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Piórkowska, Katarzyna L., Szmatoła, Tomasz, Pawlina-Tyszko, Klaudia, Gurgul, Artur, Żak, Grzegorz, and Ropka-Molik, Katarzyna M.
- Subjects
MESSENGER RNA ,DEPENDENTS ,MITOCHONDRIAL proteins ,SKELETAL muscle ,MUSCLE mass ,MICRORNA - Abstract
The search is still on for the molecular processes associated with the development and metabolism of skeletal muscles. Selection conducted in farm animals is focused on high muscle mass because it delivers higher economic profit. The present study aimed to shed light on mRNA degradome signals that could be characteristic for molecular processes associated with an abundance of muscle mass and to identify miRNA regulatory networks controlling these processes in pigs applying next-generation-sequencing (NGS). In the study, over 10,000 degraded transcripts were identified per sample, with the highest abundance for genes encoding mitochondrial proteins (COXs, NDs, CYTB, ATP6 and ATP8). Moreover, only 26% of the miRNA targets were found within this degraded transcript pool, which suggested for miRNAs other molecular mechanism at different level of gene expression than mRNA degradation. On the other hand, a small share of the identified degraded transcripts associated with miRNA regulation suggests a different mechanism of mRNA degradation for identified degraded transcropts. Subsequently, most of the miRNA gene degraded targets, such as ENO3, CKM, CRYAB and ADAM19 encode proteins involved in the muscle mass control. The present study showed an interesting dependence between miRNAs and their targets. Nevertheless, the complete view of the miRNA regulatory network could be a subject of further advanced research, which would employ a miRNA transfection procedure in skeletal muscle cell cultures. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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47. The SSC15 QTL-Rich Region Mutations Affecting Intramuscular Fat and Production Traits in Pigs.
- Author
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Piórkowska, Katarzyna, Tyra, Mirosław, Żukowski, Kacper, and Ropka-Molik, Katarzyna
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SWINE ,GENETIC markers ,WILD boar ,NUCLEOTIDE sequence ,NON-coding RNA ,LINCRNA - Abstract
One of the more interesting regions in the pig genome is on chromosome 15 (115,800,000-122,100,000, SSC15, Sus scrofa 11.1) that has high quantitative trait locus (QTL) density associated with fattening, slaughter and meat quality characteristics. The SSC15 region encodes over 80 genes and a few miRNA sequences where potential genetic markers can be found. The goal of the study was to evaluate the effects of SSC15 mutations associated with villin 1 (VIL1), tensin 1 (TNS1), obscurin-like 1 (OBSL1) genes and with one long non-coding RNA (lncRNA) on productive pig traits and to enrich the genetic marker pool in further selection purpose. The potential genetic markers were identified using the targeted enrichment DNA sequencing (TEDNA-seq) of chromosome 15 region. The selected mutations were genotyped by using HRM, PCR and PCRRFLP methods. The association study was performed using the general linear model (GLM) in the sas program that included over 600 pigs of 5 Polish populations. The rs332253419 VIL1 mutation shows a significant effect on intramuscular fat (IMF) content in Duroc population where AA pigs had a 16% higher level than heterozygotes. The IMF content is also affected by the OBSL1 mutation, and the differences between groups are even up to 30%, but it is strongly dependent on breed factor. The OBSL1 mutation also significantly influences the meat yellowness, backfat thickness and pH level. The performed study delivers valuable information that could be highly useful during the development of the high-throughput genotyping method for further selection purposes in pigs. The OBSL1 and VIL1 mutations seem to be the most promising DNA marker showing a high effect on IMF level. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
48. Inter- and intrabreed diversity of the major histocompatibility complex (MHC) in primitive and draft horse breeds.
- Author
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Jaworska, Joanna, Ropka-Molik, Katarzyna, Wocławek-Potocka, Izabela, and Siemieniuch, Marta
- Subjects
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HORSE breeds , *MAJOR histocompatibility complex , *HETEROZYGOSITY , *BREEDING , *GENETIC recombination , *SHOW horses , *DOMESTIC animals - Abstract
Background: Polymorphism of major histocompatibility complex (MHC) genes ensures effective immune responses against a wide array of pathogens. However, artificial selection, as performed in the case of domestic animals, may influence MHC diversity. Here, we investigate and compare the MHC diversity of three populations of horses, for which different breeding policies were applied, to evaluate the impact of artificial selection and the environment on MHC polymorphism. Methods: Samples of DNA were taken from 100 Polish draft horses, 38 stabled Konik Polski horses and 32 semiferal Konik Polski horses. MHC alleles and haplotype diversity within and between these populations of horses was estimated from 11 MHC microsatellite loci. Results: MHC diversity measured based on allelic richness, observed heterozygosity, expected heterozygosity and polymorphism content was similar across the MHC microsatellite loci in all three populations. The highest expected heterozygosity was detected in semiferal primitive horses (He = 0.74), while the lowest was calculated for draft horses (He = 0.65). In total, 203 haplotypes were determined (111 in Polish draft horses, 43 in semiferal Konik Polski horses and 49 in stabled Konik Polski horses), and four haplotypes were shared between the two populations of Koniks. None of these haplotypes were present in any of the previously investigated horse breeds. Intra-MHC recombination events were detected in all three populations. However, the population of semiferal Konik horses showed the highest recombination frequency among the three horse populations. In addition, three recombination events were detected. Conclusions: These results showed that despite the different breeding policies, the MHC allele and haplotype diversity was similarly high in all three horse populations. Nevertheless, the proportion of new haplotypes in the offspring was the highest in semiferal Konik Polski horses, which indicates the influence of the environment on MHC diversity in horses. Thus, we speculate that the genetic makeup of the domestic horse MHC might be more strongly influenced by the environment than by artificial selection. Moreover, intra-MHC conversion, insertion, and deletion and intra-MHC recombination may be proposed as mechanisms underlying the generation of new MHC haplotypes in horses. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
49. Performance Traits of Puławska Pigs Depending on Polymorphism in the RYR1 Gene (C.1843C>T).
- Author
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Szyndler-Nędza, Magdalena, Ropka-Molik, Katarzyna, Mucha, Aurelia, Blicharski, Tadeusz, and Babicz, Marek
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- *
PIGLETS , *ANIMAL carcasses , *SWINE , *ANIMAL herds , *ULTRASONIC equipment , *ANIMAL populations , *GENES - Abstract
The objective of the study was to determine the relationship between polymorphism in the RYR1 gene (rs118192172) and on-farm test results (fattening, slaughter and reproductive traits) of pigs maintained in conservation herds. the experiment covered 76 boars and 291 gilts kept in 33 herds. animals aged 150–210 days were weighed and P2 and P4 backfat thickness as well as P4 loin muscle depth (P4m) were measured with an ultrasonic device. standardised daily gain (g/day) of the animals and carcass meat percentage (%) were determined. the following reproductive traits of sows were also collected: number of teats, age at first farrowing, dates of next farrowing, number of piglets born alive and weaned at 21 days of age. Hair roots were sampled from the gilts, sows and boars to determine the RYR1 gene polymorphism. it was found that only 3% of the animals in the analysed population had TT genotypes, whereas 28.34% were heterozygous (CT). this polymorphism (CT) was carried by 28.34% of the animals. when analysing the effect of this polymorphism on fattening and slaughter traits in live animals, it was found that sows with TT genotype, compared to the others, were characterised by higher backfat thickness only (P≤0.05). In the boars with CC and CT genotypes, no significant differences were noted between the values of the analysed traits. for reproductive traits of the sows, it was observed that females with TT genotype weaned more piglets until 21 days of age (P≤0.05). [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
50. Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development.
- Author
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Piórkowska, Katarzyna, Żukowski, Kacper, Połtowicz, Katarzyna, Nowak, Joanna, Wojtysiak, Dorota, Derebecka, Natalia, Wesoły, Joanna, and Ropka-Molik, Katarzyna
- Subjects
HYPOTHALAMUS ,AUTONOMIC nervous system ,TRANSCRIPTOMES ,SOMATOTROPIN ,CHICKENS - Abstract
The hypothalamus plays an overarching role that is reflected in the physiological processes observed in the entire organism. The hypothalamus regulates selected metabolic processes and activities of the autonomic nervous system. The avian hypothalamus due to the structural complexity is not well described and has a slightly different function than the mammalian hypothalamus that is the subject of numerous studies. The present study evaluated activities of hypothalamic genes in fast-growing chickens during development (at the 1
st day and 3rd and 6th weeks after hatching). The hypothalamic transcriptomes for 3- and 6-week-old cockerels were analysed using an RNA sequencing method in next-generation sequencing (NGS) technology. The differentially expressed gene analysis was conducted using DESeq2 software. In younger 22-day-old cockerels, 389 genes showed higher expression (fold change > 1.5) than that in 45-day-old birds. These genes played a role in several biological processes because they encoded proteins involved in integrin signalling, regulation of hormone levels, camera-type eye development, and blood vessel development. Moreover, surprisingly in the hypothalamus of 3-week-old cockerels, transcripts were identified for proteins involved in both anorexigenic (POMC, NMU) and orexigenic (PMCH, ALDH1A1, LPL, and GHRH) pathways. The RNA-seq results were confirmed by qPCR methods. In summary, the intensive growth of 3-week-old chickens was reflected in hypothalamic activities because the genes associated with the somatotropin axis and regulation of satiety centre showed increased expression. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
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